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https://www.readbyqxmd.com/read/29775424/optimized-yeast-based-in-vitro-bioassay-for-determination-of-estrogenic-and-androgenic-activity-of-hydroxylated-methoxylated-metabolites-of-bdes-cbs-and-related-lipophilic-organic-pollutants
#1
Marek Roszko, Marta Kamińska, Krystyna Szymczyk, Katarzyna Piasecka-Jóźwiak, Beata Chabłowska
Persistent organic pollutants (POPs) are known to show endocrine disrupting (ED) activity, including interactions with hormone receptors. The aim of this work was to develop a bioassay applicable for evaluation of ED potency of highly lipophilic metabolites of POPs. To that end, a yeast-based bio-assay protocol was used. Estrogenic / androgenic activity of some native brominated biphenyl ethers (BDEs) / chlorinated biphenyls (CBs), and their hydroxylated / methoxylated metabolites was assessed. Since data (including potency compared to reference native hormones) obtained using different protocols vary, the possibility that yeast transforms POPs into some more potent compounds was first checked; it seems that no such transformation is important from the test applicability standpoint...
May 18, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/29775322/prediction-of-human-cytochrome-p450-inhibition-using-a-multi-task-deep-autoencoder-neural-network
#2
Xiang Li, Youjun Xu, Luhua Lai, Jianfeng Pei
Adverse side effects of drug-drug interactions induced by human cytochrome P450 (CYP450) inhibition is an important consideration in drug discovery. It is highly desirable to develop computational models that can predict the inhibitive effect of a compound against a specific CYP450 isoform. In this study, we developed a multi-task model for concurrent inhibition prediction of five major CYP450 isoforms, namely 1A2, 2C9, 2C19, 2D6, and 3A4. The model was built by training multi-task autoencoder deep neural network (DNN) on a large dataset containing more than 13000 compounds, extracted from PubChem BioAssay Database...
May 18, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29774292/evaluation-of-phytosynthesised-silver-nanoparticles-from-leaf-extracts-of-leucas-aspera-and-hyptis-suaveolens-and-their-larvicidal-activity-against-malaria-dengue-and-filariasis-vectors
#3
Devan Elumalai, Maduraiveeran Hemavathi, Chandrasekar Vijayalakshmi Deepaa, Patheri Kunyil Kaleena
The present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of Leucas aspera and Hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized by Ultraviolet and visible absorption spectroscopy (UV), Fourier transform-infrared spectroscopy (FT-IR), X-ray spectroscopy (XRD), Field emission scanning electron microscope (FESEM) and High-resonance transmission electron microscopy (HRTEM) analysis...
November 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29773898/new-antioxidants-from-the-culture-broth-of-hericium-coralloides
#4
Ji-Yul Kim, E-Eum Woo, In-Kyoung Lee, Bong-Sik Yun
In our effort to find antioxidants from the higher fungi, we isolated three new compounds (1-3) with a known compound, spirobenzofuran (4), from the culture broth of Hericium coralloides. Bioassay-guided fractionation led to the isolation of these compounds, and we determined the chemical structures through spectroscopic methods. These compounds exhibited antioxidant activity in the range of IC50 values of 29-66 μM in the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging assay...
May 17, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29772647/the-antimicrobial-activity-of-annona-emarginata-schltdl-h-rainer-and-most-active-isolated-compounds-against-clinically-important-bacteria
#5
Juan G Dolab, Beatriz Lima, Ewelina Spaczynska, Jiri Kos, Natividad H Cano, Gabriela Feresin, Alejandro Tapia, Francisco Garibotto, Elisa Petenatti, Monica Olivella, Robert Musiol, Josef Jampilek, Ricardo D Enriz
Annona emarginata (Schltdl.) H. Rainer, commonly known as "arachichú", "araticú", "aratigú", and "yerba mora", is a plant that grows in Argentina. Infusions and decoctions are used in folk medicine as a gargle against throat pain and for calming toothache; another way to use the plant for these purposes is chewing its leaves. Extracts from bark, flowers, leaves, and fruits from A. emarginata were subjected to antibacterial assays against a panel of Gram (+) and Gram (-) pathogenic bacteria according to Clinical and Laboratory Standards Institute protocols...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29772543/functional-diagnostics-for-thyrotropin-hormone-receptor-autoantibodies-bioassays-prevail-over-binding-assays
#6
Simon David Lytton, Anke Schluter, Paul J Banga
Autoantibodies to the thyrotropin hormone receptor (TSH-R) are directly responsible for the hyperthyroidism in Graves' disease and mediate orbital manifestations in Graves' orbitopathy (otherwise known as thyroid eye disease). These autoantibodies are heterogeneous in their function and collectively referred to as TRAbs. Measurement of TRAbs is clinically important for diagnosis of a variety of conditions and different commercial assays with high sensitivity and specificity are available for diagnostic purposes...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29772241/two-new-cytotoxic-steroids-from-the-chinese-soft-coral-sinularia-sp
#7
Song-Wei Li, Wen-Ting Chen, Li-Gong Yao, Yue-Wei Guo
Two new steroids, ximaosteroid E (1) and ximaosteroid F (2), along with two known related compounds (3 and 4), were isolated from the Chinese soft coral Sinularia sp. Notably, 1 possesses an uncommon dihydrofuran group. Their structures were established from extensive spectroscopic analyses and comparisons of their spectral data with those reported in the literature. The absolute configuration of 2 was determined by applying the modified Mosher's method. In bioassay, compounds 1, 2, and 4 showed significant cytotoxicity against the HL-60 tumor cell line with IC50 values of 1...
May 14, 2018: Steroids
https://www.readbyqxmd.com/read/29772116/bioassay-guided-isolation-of-antifungal-compounds-from-disporopsis-aspera-engl-against-pseudoperonospora-cubensis-and-phytophthora-infestans
#8
Feng Zhu, Chengshan Yuan, Fangli Gang, Chaofu Yang, Wenjun Wu, Jiwen Zhang
Oomycetes are one type of the most highly destructive of the diseases that cause damage to some important crop plants, such as potato late blight, cucumber downy mildew and grape downy mildew. As main approach of the ongoing search for new botanical fungicide from plant, the secondary metabolites of D. aspera were investigated. Through efficient bioassay-guided isolation, two new (1, 2), twelve known compounds (3-14) were isolated, and their structures were determined via extensive NMR, HR-ESI-MS, IR. They were isolated from this genus for the first time except for compounds 11, 12...
May 17, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29771755/thyroid-stimulating-hormone-receptor-antibodies-in-thyroid-eye-disease-methodology-and-clinical-applications
#9
Tanja Diana, George J Kahaly
BACKGROUND: Thyroid stimulating hormone receptor antibodies (TSHR-Ab) cause autoimmune hyperthyroidism and are prevalent in patients with related thyroid eye disease (TED). PURPOSE: To provide a historical perspective on TSHR-Ab and to present evidence-based recommendations for clinical contemporary use. METHODS: The authors review the recent literature pertaining to TSHR-Ab in patients with TED and describe the various immunoassays currently used for detecting TSHR-Ab and their clinical applications...
May 2, 2018: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29771641/inhabiting-plant-roots-nematodes-and-truffles-polyphilus-a-new-helotialean-genus-with-two-globally-distributed-species
#10
Samad Ashrafi, Dániel G Knapp, Damien Blaudez, Michel Chalot, Jose G Maciá-Vicente, Imre Zagyva, Abdelfattah A Dababat, Wolfgang Maier, Gábor M Kovács
Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail...
March 26, 2018: Mycologia
https://www.readbyqxmd.com/read/29770906/volatile-and-contact-chemical-cues-associated-with-host-and-mate-recognition-behavior-of-sphenophorus-venatus-and-sphenophorus-parvulus-coleoptera-dryophthoridae
#11
Alexandra G Duffy, Gabriel P Hughes, Matthew D Ginzel, Douglas S Richmond
Beetles in the genus Sphenophorus Schönherr, or billbugs, potentially utilize both volatile and non-volatile behavior-modifying chemical signals. These insects are widely distributed across North America, often occurring in multi-species assemblages in grasses. However, details about their host- and mate-finding behavior are poorly understood. This study tested the hypothesis that volatile organic compounds from host-plants and conspecifics direct the dispersal behavior of hunting billbug S. venatus Say. Further, we characterized the cuticular hydrocarbon profiles of two widespread pest species, S...
May 17, 2018: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/29769137/laboratory-studies-on-the-oviposition-stimuli-of-culicoides-stellifer-diptera-ceratopogonidae-a-suspected-vector-of-orbiviruses-in-the-united-states
#12
Dinesh Erram, Nathan Burkett-Cadena
BACKGROUND: Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) exert a significant impact on animal agriculture worldwide because they transmit bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) to ruminants. Without effective vaccines, BTV/EHDV vector management strategies are needed, particularly in commercial white-tailed deer (WTD) facilities. However, detailed information on the ecology of midge immatures in/around cervid operations is currently lacking...
May 16, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29769066/phenolic-profile-and-biological-properties-of-the-leaves-of-ficus-vasta-forssk-moraceae-growing-in-egypt
#13
Maria Fernanda Taviano, Khaled Rashed, Angela Filocamo, Francesco Cacciola, Paola Dugo, Luigi Mondello, Carlo Bisignano, Rosaria Acquaviva, Manuela D'Arrigo, Natalizia Miceli
BACKGROUND: Ficus vasta Forssk. (Moraceae) is traditionally used for the treatment of various ailments; nonetheless, this species has been poorly studied to date. This work aimed to characterize the phenolic profile and to evaluate the antioxidant and antimicrobial properties of a hydroalcoholic extract obtained from F. vasta leaves collected in Egypt. METHODS: The phenolic profile of the extract was characterized by HPLC-PDA/ESI-MS. The antioxidant properties were examined by different in vitro systems: DPPH test, reducing power and metal chelating activity assays...
May 16, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29768234/improved-experimental-time-of-ultra-large-bioassays-using-a-parallelised-microfluidic-biochip-architecture-scheduling
#14
Maryam Taajobian, Ali Jahanian
Digital microfluidic is an emerging technology to reduce the cost and time of experiments and improve the flexibility, automate-ability and correctness of biochemical assays. In many of applications such as drug discovery and DNA profiling, a large number of bio-operations (e.g. the chemical operations used in biology applications) must be done. In these applications, parallelising the operations will be critical in accuracy and cost of the process and digital microfluidic biochips can be considered as a reasonable platform...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29767512/azide-and-dye-conjugated-coelenterazine-analogues-for-a-multiplex-molecular-imaging-platform
#15
Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Satoshi Kishigami, Naoko Iwasawa, Shin-Ichi Sasaki, Takahiro Nakajima, Moritoshi Sato, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
Native coelenterazine (nCTZ) is a common substrate to most marine luciferases and photoproteins. In this study, nine novel dye- and azide-conjugated CTZ analogues were synthesized by conjugating a series of fluorescent dyes or an azide group to the C-2 or C-6 position of the nCTZ backbone to obtain bulkiness-driven substrate specificity and potential chemiluminescence/bioluminescence resonance energy transfer (C/BRET). The investigation on the optical properties revealed that azide-conjugated CTZs emit greatly biased bioluminescence to ALucs and ca...
May 16, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29766799/anticancer-activity-of-an-oplopanax-elatus-stem-extract-and-biologically-active-isolated-compounds
#16
Jinfeng Yang, Yea-Ji Lee, Yong Soo Kwon, Myong Jo Kim
BACKGROUND AND METHODS: Oplopanax elatus (Nakai) Nakai is used in folk medicine in China. In this study, the antiproliferative activity of an O. elatus fraction extracted by ethyl acetate (EF) was tested on human breast cancer MCF-7 cells, human colon cancer HCT-116 cells, and human stomach cancer AGS cells. The potential mechanism of antiproliferation was also investigated using an apoptosis assay. RESULTS: The results showed that the EF remarkably suppressed proliferation of human breast, stomach, and colon cancer cells...
May 14, 2018: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29766638/lethal-and-sublethal-effects-of-chlorantraniliprole-on-spodoptera-cosmioides-lepidoptera-noctuidae
#17
Alejandra L Lutz, Isabel Bertolaccini, Roberto R Scotta, María C Curis, María A Favaro, Laura N Fernandez, Daniel E Sánchez
BACKGROUND: Spodoptera cosmioides (Walker, 1858) population has increased in Bt soybean crops in Argentina. As there are no registered products for its control, the recommended insecticides for S. frugiperda are used. The aim of this study was therefore to determine the lethal concentration (LC) and sublethal effects of chlorantraniliprole on the biological and reproductive functions of S. cosmioides, an emerging soybean pest in Argentina. RESULTS: An ingestion toxicity bioassay showed that chlorantraniliprole was active against larvae of the second instar, and after 48 h exposure LC50 was 0...
May 16, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29766399/consensus-guidelines-for-the-use-and-interpretation-of-angiogenesis-assays
#18
REVIEW
Patrycja Nowak-Sliwinska, Kari Alitalo, Elizabeth Allen, Andrey Anisimov, Alfred C Aplin, Robert Auerbach, Hellmut G Augustin, David O Bates, Judy R van Beijnum, R Hugh F Bender, Gabriele Bergers, Andreas Bikfalvi, Joyce Bischoff, Barbara C Böck, Peter C Brooks, Federico Bussolino, Bertan Cakir, Peter Carmeliet, Daniel Castranova, Anca M Cimpean, Ondine Cleaver, George Coukos, George E Davis, Michele De Palma, Anna Dimberg, Ruud P M Dings, Valentin Djonov, Andrew C Dudley, Neil P Dufton, Sarah-Maria Fendt, Napoleone Ferrara, Marcus Fruttiger, Dai Fukumura, Bart Ghesquière, Yan Gong, Robert J Griffin, Adrian L Harris, Christopher C W Hughes, Nan W Hultgren, M Luisa Iruela-Arispe, Melita Irving, Rakesh K Jain, Raghu Kalluri, Joanna Kalucka, Robert S Kerbel, Jan Kitajewski, Ingeborg Klaassen, Hynda K Kleinmann, Pieter Koolwijk, Elisabeth Kuczynski, Brenda R Kwak, Koen Marien, Juan M Melero-Martin, Lance L Munn, Roberto F Nicosia, Agnes Noel, Jussi Nurro, Anna-Karin Olsson, Tatiana V Petrova, Kristian Pietras, Roberto Pili, Jeffrey W Pollard, Mark J Post, Paul H A Quax, Gabriel A Rabinovich, Marius Raica, Anna M Randi, Domenico Ribatti, Curzio Ruegg, Reinier O Schlingemann, Stefan Schulte-Merker, Lois E H Smith, Jonathan W Song, Steven A Stacker, Jimmy Stalin, Amber N Stratman, Maureen Van de Velde, Victor W M van Hinsbergh, Peter B Vermeulen, Johannes Waltenberger, Brant M Weinstein, Hong Xin, Bahar Yetkin-Arik, Seppo Yla-Herttuala, Mervin C Yoder, Arjan W Griffioen
The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation...
May 15, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29765513/20-s-protopanaxadiol-regio-selectively-targets-androgen-receptor-anticancer-effects-in-castration-resistant-prostate-tumors
#19
Mohamed Ben-Eltriki, Subrata Deb, Mohamed Hassona, Gray Meckling, Ladan Fazli, Mei Yieng Chin, Nada Lallous, Takeshi Yamazaki, William Jia, Paul S Rennie, Artem Cherkasov, Emma S Tomlinson Guns
We have explored the effects of 20(S)-protopanaxadiol (aPPD), a naturally derived ginsenoside, against androgen receptor (AR) positive castration resistant prostate cancer (CRPC) xenograft tumors and have examined its interactions with AR. In silico docking studies for aPPD binding to AR, alongside transactivation bioassays and in vivo efficacy studies were carried out in the castration-resistant C4-2 xenograft model. Immunohistochemical (IHC) and Western blot analyses followed by evaluation of AR, apoptotic, cell cycle and proliferative markers in excised tumors was performed...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29765490/antiaging-of-cucurbitane-glycosides-from-fruits-of-momordica-charantia-l
#20
Xueli Cao, Yujuan Sun, Yanfei Lin, Yanjun Pan, Umer Farooq, Lan Xiang, Jianhua Qi
Methanol extracts of Momordica charantia L. fruits are extensively studied for their antiaging activities. A new cucurbitane-type triterpenoid (1) and nine other known compounds (2-10) were isolated, and their structures were determined according to their spectroscopic characteristics and chemical derivatization. Biological evaluation was performed on a K6001 yeast bioassay system. The results indicated that all the compounds extended the replicative lifespan of K6001 yeast significantly. Compound 9 was used to investigate the mechanism involved in the increasing of the lifespan...
2018: Oxidative Medicine and Cellular Longevity
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