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https://www.readbyqxmd.com/read/27923250/clinical-evaluation-of-the-first-automated-assay-for-the-detection-of-stimulating-tsh-receptor-autoantibodies
#1
S Allelein, M Ehlers, S Goretzki, D Hermsen, J Feldkamp, M Haase, T Dringenberg, C Schmid, H Hautzel, M Schott
Until recently, stimulating TSH receptor autoantibodies (sTRAbs) could only be measured by bioassays. A new assay system, which directly detects sTRAb in sera by applying bridge technology, has been established and is now available as automated chemiluminescence (bridge) immunoassay. We evaluated the automated bridge assay in clinical routine and compared it with a conventional automated TRAb assay (competition assay). Altogether, 226 Graves' disease (GD), 57 autoimmune thyroiditis, 74 non-autoimmune nodular goiter and 49 thyroid cancer patients, as well as 41 healthy controls were retrospectively evaluated...
December 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/27921398/effects-of-rhizobacteria-paenibacillus-polymyxa-apec136-and-bacillus-subtilis-apec170-on-biocontrol-of-postharvest-pathogens-of-apple-fruits
#2
Young Soo Kim, Kotnala Balaraju, Yongho Jeon
In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27920785/a-jasmonate-inducible-defense-trait-transferred-from-wild-into-cultivated-tomato-establishes-increased-whitefly-resistance-and-reduced-viral-disease-incidence
#3
Rocío Escobar-Bravo, Juan M Alba, Clara Pons, Antonio Granell, Merijn R Kant, Enrique Moriones, Rafael Fernández-Muñoz
Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated tomatoes lack many of the resistances of their wild relatives. In order to increase protection to its major pest, the whitefly Bemisia tabaci and its transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from the wild tomato Solanum pimpinellifolium into cultivated tomato, Solanum lycopersicum. The tomato backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike cultivated tomatoes, and exhibits increased, yet limited protection to whiteflies at early development stages...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27916889/hyaluronic-acid-gel-based-scaffolds-as-potential-carrier-for-growth-factors-an-in-vitro-bioassay-on-its-osteogenic-potential
#4
Masako Fujioka-Kobayashi, Benoit Schaller, Eizaburo Kobayashi, Maria Hernandez, Yufeng Zhang, Richard J Miron
Hyaluronic acid (HA) has been utilized for a variety of regenerative medical procedures due to its widespread presence in connective tissue and perceived biocompatibility. The aim of the present study was to investigate HA in combination with recombinant human bone morphogenetic protein 9 (rhBMP9), one of the most osteogenic growth factors of the BMP family. HA was first combined with rhBMP9 and assessed for the adsorption and release of rhBMP9 over 10 days by ELISA. Thereafter, ST2 pre-osteoblasts were investigated by comparing (1) control tissue culture plastic, (2) HA alone, and (3) HA with rhBMP9 (100 ng/mL)...
November 30, 2016: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/27916862/contrasting-roles-of-deoxynivalenol-and-nivalenol-in-host-mediated-interactions-between-fusarium-graminearum-and-sitobion-avenae
#5
Jassy Drakulic, Mohd Haziq Kahar, Olubukola Ajigboye, Toby Bruce, Rumiana V Ray
Fusarium graminearum is the predominant causal species of Fusarium head blight in Europe and North America. Different chemotypes of the species exist, each producing a plethora of mycotoxins. Isolates of differing chemotypes produce nivalenol (NIV) and deoxynivalenol (DON), which differ in toxicity to mammals and plants. However, the effect of each mycotoxin on volatile emissions of plant hosts is not known. Host volatiles are interpreted by insect herbivores such as Sitobion avenae, the English grain aphid, during host selection...
November 30, 2016: Toxins
https://www.readbyqxmd.com/read/27916120/development-and-statistical-assessment-of-a-paper-based-immunoassay-for-detection-of-tumor-markers
#6
Thiago Mazzu-Nascimento, Giorgio Gianini Morbioli, Luis Aparecido Milan, Fabiana Cristina Donofrio, Carlos Alberto Mestriner, Emanuel Carrilho
Paper-based assays are an attractive low-cost option for clinical chemistry testing, due to characteristics such as short time of analysis, low consumption of samples and reagents, and high portability of assays. However, little attention has been given to the evaluation of the performance of these simple tests, which should include the use of a statistical approach to define the choice of best cut-off value for the test. The choice of the cut-off value impacts on the sensitivity and specificity of the bioassay...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27916114/competitive-inhibition-assay-for-the-detection-of-progesterone-in-dairy-milk-using-a-fiber-optic-spr-biosensor
#7
D Daems, J Lu, F Delport, N Mariën, L Orbie, B Aernouts, I Adriaens, T Huybrechts, W Saeys, D Spasic, J Lammertyn
Analytical methods that are often used for the quantification of progesterone in bovine milk include immunoassays and chromatographic techniques. Depending on the selected method, the main disadvantages are the cost, time-to-result, labor intensity and usability as an automated at-line device. This paper reports for the first time on a robust and practical method to quantify small molecules, such as progesterone, in complex biological samples using an automated fiber optic surface plasmon resonance (FO-SPR) biosensor...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27914919/expressing-a-moth-abcc2-gene-in-transgenic-drosophila-causes-susceptibility-to-bt-cry1ac-without-requiring-a-cadherin-like-protein-receptor
#8
Tristan Stevens, Sisi Song, John B Bruning, Amanda Choo, Simon W Baxter
Bt toxins ingested by insect pests can bind to midgut receptors and cause death, although several steps in this process remain unclear. Multiple Bt toxin receptors have been identified in Lepidoptera, including a cadherin-like protein (CaLP), which is central to several models explaining Bt toxins' mode of action. Mutations in the Plutella xylostella ATP-dependent binding cassette transporter C2 (Px-abcc2), rather than CaLP, are genetically linked with Bt Cry1Ac resistance. Here we expressed Px-abcc2 in Drosophila and performed larval bioassays to determine whether this protein acts as an effective Bt receptor...
November 30, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27914797/design-synthesis-and-fungicidal-activity-of-novel-2-substituted-aminocycloalkylsulfonamides
#9
Caixiu Liu, Xiaojing Yan, Minlong Wang, Peiwen Qin, Zhiqiu Qi, Mingshan Ji, Xingyu Liu, P Vijaya Babu, Xinghai Li, Zi-Ning Cui
A series of novel 2-substituted aminocycloalkylsulfonamides were designed and synthesized by highly selective N-alkylation reaction, whose structures were characterized by (1)H NMR, (13)C NMR and HRMS. Among them, the configuration of compounds III12 and III20 were confirmed by X-ray single crystal diffraction. Bioassays demonstrated that the title compounds had considerable effects on different strains of Botrytis cinerea and Pyricularia grisea. Comparing with positive control procymidone (EC50=10.31mg/L), compounds III28, III29, III30 and III31 showed excellent fungicidal activity against a strain of B...
November 23, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27914535/indoxacarb-biotransformation-in-the-german-cockroach
#10
Ameya D Gondhalekar, Ernesto S Nakayasu, Isabel Silva, Bruce Cooper, Michael E Scharf
Insecticides that are used for pest control undergo physical and biological (enzymatic) degradation. Indoxacarb is an oxadiazine class sodium channel blocker insecticide used for German cockroach (Blattella germanica L.) control. At present, no information is available on enzymatic biotransformation or metabolism of indoxacarb in this important urban pest. We studied the biotransformation pathways of indoxacarb in one susceptible and three field strains with varying susceptibility levels using liquid chromatography and high-resolution mass spectrometry...
November 2016: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/27913018/potential-impact-of-flowback-water-from-hydraulic-fracturing-on-agricultural-soil-quality-metal-metalloid-bioaccessibility-microtox-bioassay-and-enzyme-activities
#11
Season S Chen, Yuqing Sun, Daniel C W Tsang, Nigel J D Graham, Yong Sik Ok, Yujie Feng, Xiang-Dong Li
Hydraulic fracturing has advanced the development of shale gas extraction, while inadvertent spills of flowback water may pose a risk to the surrounding environment due to its high salt content, metals/metalloids (As, Se, Fe and Sr), and organic additives. This study investigated the potential impact of flowback water on four representative soils from shale gas regions in Northeast China using synthetic flowback solutions. The compositions of the solutions were representative of flowback water arising at different stages after fracturing well establishment...
November 29, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27912907/bioactivity-guided-isolation-of-anti-inflammatory-triterpenoids-from-the-sclerotia-of-poria-cocos-using-lps-stimulated-raw264-7-cells
#12
Seoung Rak Lee, Seulah Lee, Eunjung Moon, Hye-Jin Park, Hyun Bong Park, Ki Hyun Kim
Poria cocos Wolf (Polyporaceae) has been used as a medicinal fungus to treat various diseases since ancient times. This study aimed to investigate the anti-inflammatory chemical constituents of the sclerotia of P. cocos. Based on bioassay-guided fractionation using lipopolysaccharide (LPS)-stimulated Raw264.7 cells, chemical investigation of the EtOH extract of the sclerotia of P. cocos resulted in the isolation and identification of eight compounds including six triterpenoids, namely poricoic acid A (1), 3-O-acetyl-16α-hydroxydehydrotrametenolic acid (2), polyporenic acid C (3), 3β-hydroxylanosta-7,9(11),24-trien-21-oic acid (4), trametenolic acid (5), and dehydroeburicoic acid (6), as well as (-)-pinoresinol (7) and protocatechualdehyde (8)...
November 23, 2016: Bioorganic Chemistry
https://www.readbyqxmd.com/read/27910784/hepatoprotective-effect-of-tricholoma-giganteum-agaricomycetes-in-a-nonalcoholic-fatty-liver-disease-rat-model
#13
Ramar Govindaraj, Michael Gabriel Paulraj, Erenius Toppo, Perumal Pandikumar, Savarimuthu Ignacimuthu, Naif Abdhullah Al-Dhabi
Different concentrations of standardized ethanolic extract from the basidiocarps of Tricholoma giganteum Massee (TgEtOH) were screened for hepatoprotective effects in an animal model of rats with nonalcoholic fatty liver disease (NAFLD) fed a high-fat and high-fructose diet. After 4 weeks of treatment with TgEtOH, the relative liver weights, serum lipid concentrations, and biochemical profiles were found to be normal in treated animals compared with those given a standard drug. The macroscopic and histopathological studies clearly indicated that 200 mg/kg of ethanolic extract was effective in ameliorating the abnormalities of NAFLD...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910106/identification-of-semiochemicals-attractive-to-simulium-vittatum-is-7
#14
G G Verocai, T W McGAHA, J P Iburg, C R Katholi, E W Cupp, R Noblet, T R Unnasch
Many blackfly species (Diptera: Simuliidae) are economically important insect pests, both as nuisance biters and as vectors of pathogens of medical and veterinary relevance. Among the important blackfly pest species in North America is Simulium vittatum Zetterstedt sensu lato. The objective of this study was to identify compounds excreted by mammalian hosts that are attractive to host-seeking S. vittatum females. The attractiveness of putative compounds to colonized S. vittatum was tested through electrophysiological (electroantennography; n = 58 compounds) and behavioural (Y-tube assays; n = 7 compounds in three concentrations) bioassays...
December 2, 2016: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/27909922/production-of-biologically-active-recombinant-goose-fsh-in-a-single-chain-form-with-a-ctp-linker-sequence
#15
Hui Li, Huanxi Zhu, Qinming Qin, Mingming Lei, Zhendan Shi
FSH is a glycoprotein hormone secreted by the pituitary gland that is essential for gonadal development and reproductive function. In avian reproduction study, especially in avian reproduction hormone study, it is hindered by the lack of biologically active FSH. In order to overcome this shortcoming, we prepared recombinant goose FSH as a single chain molecule and tested its biological activities in the present study. Coding sequences for mature peptides of goose FSH α and β subunits were amplified from goose pituitary cDNA...
December 1, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27909855/conifer-monoterpene-chemistry-during-an-outbreak-enhances-consumption-and-immune-response-of-an-eruptive-folivore
#16
Amy M Trowbridge, M Deane Bowers, Russell K Monson
Changes in the chemical composition of plant defense compounds during herbivory can impact herbivore resource allocation patterns and thereby herbivore survival, growth, and immune response against endoparasitoid infection. Few studies have investigated folivore responses to changes in plant chemistry that occur under outbreak conditions in mature conifer systems. Using data from an earlier observational field study, we carried out laboratory bioassays to test how variation in monoterpenes in piñon pine trees (Pinus edulis, Pinaceae) during an outbreak affects growth, consumption, and immune response of a specialist herbivore, the Southwestern tiger moth (Lophocampa ingens, Arctiidae)...
December 1, 2016: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/27908745/effect-of-temperature-and-search-area-on-the-functional-response-of-anisops-sardea-hemiptera-notonectidae-against-anopheles-stephensi-in-laboratory-bioassay
#17
Rajendra Prasad Mondal, Goutam Chandra, Subhasis Bandyopadhyay, Anupam Ghosh
Present study was carried out to establish the influence of abiotic factors on foraging activities of a predatory hemipteran insect Anisops sardea against Anopheles stephensi larvae. The functional response of A. sardea was evaluated in variable density of prey items with variation in search area (100, 250, 500 and 1000ml water volume) and temperatures (20, 25 and 30°C). The results of laboratory bioassay revealed that prey consumption rate of predator species was positively related with increasing temperature and inversely related with increasing search area...
November 28, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27907122/biodegradation-of-methyl-tertiary-butyl-ether-mtbe-by-a-microbial-consortium-in-a-continuous-up-flow-packed-bed-biofilm-reactor-kinetic-study-metabolite-identification-and-toxicity-bioassays
#18
Guadalupe Alfonso-Gordillo, César Mateo Flores-Ortiz, Liliana Morales-Barrera, Eliseo Cristiani-Urbina
This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11...
2016: PloS One
https://www.readbyqxmd.com/read/27905627/-pediculicide-effect-of-a-eucaliptus-globulus-l-formulation
#19
Marcia Avello, Pola Fernández, Marcos Fernández, Berta Schulz, Marta De Diego, Sigrid Mennickent, Pedro Novoa, Edgar Pastene
INTRODUCTION: Pediculosis capitis is a public health problem with a high prevalence. The emergence of parasite resistance to conventional pediculicide is of great concern worldwide. OBJECTIVE: To develop alternatives pediculicide, effective and safe, based on the essential oil of Eucaliptus globulus. METHOD: Through bioassays active concentrations ranges of the essential oil were established, and proceeded to develop a standardized, stable, pharmaceutical form, evaluating its effects on our population...
August 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/27904927/effects-of-tin-on-enzyme-activity-in-holothuria-grisea-echinodermata-holothuroidea
#20
Tatiana Miura Pereira, Lívia Pôncio Mattar, Edgar Rocha Pereira, Julia Merçon, Ary Gomes da Silva, Zilma Maria Almeida Cruz
This study evaluated the effect of tin exposure on enzyme activity in the sea cucumber (Holothuria grisea Selenka, 1867). After exposure to 0 (control), 0.04, 0.08, or 0.12 mg L(-1) tin, we tested the activities of total cholinesterase in longitudinal muscles, acid phosphatase in gonads and the respiratory tree, as well as alkaline phosphatase in the intestines during a 96-h bioassay. Regression analyses showed that all enzyme activities declined with increasing tin concentrations, except for acid phosphatase in the respiratory tree, which were similarly, inhibited at all tin concentrations...
November 30, 2016: Bulletin of Environmental Contamination and Toxicology
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