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https://www.readbyqxmd.com/read/28742969/fabrication-of-a-new-lineage-of-artificial-luciferases-from-natural-luciferase-pools
#1
Sung Bae Kim, Ryo Nishihara, Daniel Citterio, Koji Suzuki
The fabrication of artificial luciferases (ALucs) with unique optical properties can have a fundamental impact on bioassays and molecular imaging. In this study, we developed a new lineage of ALucs with unique substrate preferences by extracting consensus amino acids from the alignment of 25 copepod luciferase sequences available in published databases. The primary sequence was first created with a sequence logo generator resulting in a total of 11 sibling sequences. Phylogenetic analysis shows that the newly fabricated ALucs form an independent branch, genetically isolated from the natural luciferases and from a prior series of ALucs produced by our laboratory using a smaller basis set...
July 25, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28742863/antibacterial-activity-of-tannins-isolated-from-sapium-baccatum-extract-and-use-for-control-of-tomato-bacterial-wilt
#2
Thuy Thu Vu, Hun Kim, Vu Khac Tran, Hoang Dinh Vu, Tien Xuan Hoang, Jae Woo Han, Yong Ho Choi, Kyoung Soo Jang, Gyung Ja Choi, Jin-Cheol Kim
In the search for new antibacterial agents from natural sources, we revealed that a crude methanol extract of Sapium baccatum was highly active against Ralstonia solanacearum, a causal agent of a serious disease called bacterial wilt of tomato. The bioassay-guided fractionation of this extract resulted in the isolation of seven known active compounds, including gallic acid, methyl gallate, corilagin, tercatain, chebulagic acid, chebulinic acid, and quercetin 3-O-α-L-arabinopyranoside. Their chemical structures were determined by electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28742096/insecticide-resistance-is-mediated-by-multiple-mechanisms-in-recently-introduced-aedes-aegypti-from-madeira-island-portugal
#3
Gonçalo Seixas, Linda Grigoraki, David Weetman, José Luís Vicente, Ana Clara Silva, João Pinto, John Vontas, Carla Alexandra Sousa
BACKGROUND: Aedes aegypti is a major mosquito vector of arboviruses, including dengue, chikungunya and Zika. In 2005, Ae. aegypti was identified for the first time in Madeira Island. Despite an initial insecticide-based vector control program, the species expanded throughout the Southern coast of the island, suggesting the presence of insecticide resistance. Here, we characterized the insecticide resistance status and the underlying mechanisms of two populations of Ae. aegypti from Madeira Island, Funchal and Paúl do Mar...
July 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28741319/key-genes-of-the-sex-pheromone-biosynthesis-pathway-in-female-moths-are-required-for-pheromone-quality-and-possibly-mediate-olfactory-plasticity-in-conspecific-male-moths-in-spodoptera-litura
#4
X Lin, B Wang, Y Du
Ninety sex pheromone biosynthesis genes in Spodoptera litura were identified in transcriptome data and were investigated and classified into the following five groups: fatty acid synthase, beta oxidase, fatty acyl-coenzyme A (CoA) reductase, desaturase and acetyl-CoA acetyltransferase. Fourteen female-specific genes were identified through semi-quantitative PCR, and 15 additional genes had expression levels that were 3- to 10-fold higher in females than in males. The majority of the genes had higher expression levels in the sex pheromone glands...
July 24, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28741211/influence-of-ph-edta-fe-ii-ratio-and-microbial-culture-on-fe-ii-mediated-autotrophic-denitrification
#5
Kyriaki Kiskira, Stefano Papirio, Eric Didier van Hullebusch, Giovanni Esposito
Fe(II)-mediated autotrophic denitrification with four different microbial cultures under different pH and EDTA/Fe(II) conditions was investigated in batch bioassays. Initially, the highest nitrate removal (72%) was achieved with an activated sludge inoculum. The use of pure cultures of Pseudogulbenkiania strain 2002 and Thiobacillus denitrificans resulted in a 55 and 52% nitrate removal, respectively. No denitrification was observed for a mixed culture dominated by Thiobacillus thioparus and T. denitrificans...
July 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28740550/boosting-the-peroxidase-like-activity-of-nanostructured-nickel-by-inducing-its-3-oxidation-state-in-lanio3-perovskite-and-its-application-for-biomedical-assays
#6
Xiaoyu Wang, Wen Cao, Li Qin, Tingsheng Lin, Wei Chen, Shichao Lin, Jia Yao, Xiaozhi Zhao, Min Zhou, Cheng Hang, Hui Wei
Catalytic nanomaterials with intrinsic enzyme-like activities, called nanozymes, have recently attracted significant research interest due to their unique advantages relative to natural enzymes and conventional artificial enzymes. Among the nanozymes developed, particular interests have been devoted to nanozymes with peroxidase mimicking activities because of their promising applications in biosensing, bioimaging, biomedicine, etc. Till now, lots of functional nanomaterials have been used to mimic peroxidase...
2017: Theranostics
https://www.readbyqxmd.com/read/28738771/fumitremorgins-and-relatives-from-tremorgenic-compounds-to-valuable-anti-cancer-drugs
#7
Wolf-Rainer Abraham
BACKGROUND: Fumitremorgins are mycotoxins but can also inhibit cancer cells and reverse their drug resistance. OBJECTIVE: The bioactivity of prenylated cyclo-Trp-Pro dipeptides and their derivatives concerning their application in anti-cancer therapies will be discussed. METHOD: Reports on the discovery and assessment of this class of fungal compounds are compiled from literature using Google Scholar and PubMed. The bioactivities of the natural compounds are discussed with the aim of their improvement for cancer therapy...
July 24, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28738580/true-lab-in-a-syringe-technology-for-bioassays
#8
Lenka Hárendarčíková, Daniel Baron, Andrea Šebestová, Jan Rozsypal, Jan Petr
In our work, we introduced a novel concept of the lab-in-a-syringe tests. We solved the problem of detection in already published LIS tests by putting all the reaction and detection pads directly into the syringe barrel. We also used more layers to make the results visible for users. Two detection layouts: (i) with using rounded pads-based detection, and (ii) with using rectangular detection pads, were studied. As the proof of concept, we studied the determination of Ni(II) using dimethylglyoxime as the reagent and blocking of the interference of Fe(II)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28737700/a-novel-brominated-alkaloid-securidine-a-isolated-from-the-marine-bryozoan-securiflustra-securifrons
#9
Priyanka Michael, Kine Ø Hansen, Johan Isaksson, Jeanette H Andersen, Espen Hansen
A novel brominated alkaloid, Securidine A, was isolated from the cold water marine bryozoan Securiflustra securifrons. Securidine A was isolated using semi-preparative HPLC, and the structure was elucidated by spectroscopic methods. The isolated Securidine A was tested for cytotoxic, antibacterial, and anti-diabetic activities as well as for its potential for inhibition of biofilm formation. No significant biological activity was observed in the applied bioassays, thus expanded bioactivity profiling is required, in order to reveal any potential applications for Securidine A...
July 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737040/potential-of-small-molecule-fungal-metabolites-in-antiviral-chemotherapy
#10
Biswajit G Roy
Various viral diseases, such as acquired immunodeficiency syndrome, influenza, and hepatitis, have emerged as leading causes of human death worldwide. Scientific endeavor since invention of DNA-dependent RNA polymerase of pox virus in 1967 resulted in better understanding of virus replication and development of various novel therapeutic strategies. Despite considerable advancement in every facet of drug discovery process, development of commercially viable, safe, and effective drugs for these viruses still remains a big challenge...
July 23, 2017: Antiviral Chemistry & Chemotherapy
https://www.readbyqxmd.com/read/28735421/ammonia-released-by-streptomyces-aburaviensis-induces-droplet-formation-in-streptomyces-violaceoruber
#11
Kathrin Schmidt, Dieter Spiteller
Streptomyces violaceoruber grown in co-culture with Streptomyces aburaviensis produces an about 17-fold higher volume of droplets on its aerial mycelium than in single-culture. Physical separation of the Streptomyces strains by either a plastic barrier or by a dialysis membrane, which allowed communication only by the exchange of volatile compounds or diffusible compounds in the medium, respectively, still resulted in enhanced droplet formation. The application of molecular sieves to bioassays resulted in the attenuation of the droplet-inducing effect of S...
July 22, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28735186/development-of-a-robust-reporter-gene-assay-to-measure-the-bioactivity-of-anti-pd-1-anti-pd-l1-therapeutic-antibodies
#12
Lan Wang, Chuanfei Yu, Yalan Yang, Kai Gao, Junzhi Wang
Being regarded as the 'cancer panacea', the anti-PD-1/anti-PD-L1 monoclonal antibodies (mAbs) have become the R&D focus of biopharmaceutical industries. Several marketed such mAbs have been proved particularly effective in treating various cancers. However, the cell-based bioassay to measure the biological activities of the anti-PD-1/anti-PD-L1 mAbs as the lot release or stability test has been a great challenge to quality control laboratories due to the immunomodulating nature of the mAbs. Here, we describe the development and validation of a reporter gene assay consisting of two-cell systems to measure the bioactivity of the anti-PD-1/anti-PD-L1 mAbs...
May 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28734359/fast-multiplex-analysis-of-antibodies-in-complex-sample-matrix-using-the-microfluidic-evalution%C3%A2-platform
#13
Karen Leirs, Pelin Leblebici, Jeroen Lammertyn, Dragana Spasic
Biosensor development requires comprehensive research for establishing the optimal bioassay conditions that determine the final kinetics, sensitivity and specificity. Different systems have been developed to study bioreceptor-target interactions but they often have drawbacks, such as long hands-on time, low throughput, high sample consumption and high cost. In this work, the potential of the novel microfluidic Evalution™ platform has been evaluated for developing sandwich-based assays in a fast and high-throughput fashion...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734260/time-averaged-concentrations-are-effective-for-predicting-chronic-toxicity-of-varying-copper-pulse-exposures-for-two-freshwater-green-algae-species
#14
Brad M Angel, Stuart L Simpson, Ellissah Granger, Kathryn Goodwyn, Dianne F Jolley
Intermittent, fluctuating and pulsed contaminant discharges may result in organisms receiving highly variable contaminant exposures. This study investigated the effects of dissolved copper pulse concentration and exposure duration on the toxicity to two freshwater green algae species. The effects of single copper pulses of between 1 and 48 h duration and continuous exposures (72 h) on growth rate inhibition of Pseudokirchneriella subcapitata and Chlorella sp. were compared on a time-averaged concentration (TAC) basis...
July 19, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28734106/insecticide-resistance-in-trialeurodes-vaporariorum-populations-and-novel-diagnostics-for-kdr-mutations
#15
Despoina Evr Kapantaidaki, Eldem Sadikoglou, Dimitra Tsakireli, Vasileios Kampanis, Marianna Stavrakaki, Corinna Schorn, Aris Ilias, Maria Riga, George Tsiamis, Ralf Nauen, George Skavdis, John Vontas, Anastasia Tsagkarakou
BACKGROUND: Neonicotinoids, pyrethroids and ketoenols, are currently used for the control of Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). In this study, insecticide resistance status and mechanisms were investigated by classical bioassays and molecular techniques. RESULTS: Dose response bioassays were performed on 19 Greek populations, among the 35 different whitefly populations used for the whole analysis. Resistance factors scaled up to 207-, 4657- and 59- fold, for imidacloprid, bifenthrin and spiromesifen, respectively...
July 22, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28733676/after-infection-with-leishmania-infantum-golden-hamsters-mesocricetus-auratus-become-more-attractive-to-female-sand-flies-lutzomyia-longipalpis
#16
T M Nevatte, R D Ward, L Sedda, J G C Hamilton
In Brazil, human and canine visceral leishmaniasis is caused by infection with Leishmania infantum, a Protist parasite transmitted by blood-feeding female Lutzomyia longipalpis sand flies. The objective of this study was to determine if the odour of hamsters, infected with Le. infantum, was more attractive than the odour of the same hamsters, before they were infected. The attractiveness of odour collected from individual hamsters (n = 13), before they were infected, was compared in a longitudinal study, with the attractiveness of the odour of the same hamster in a Y-tube olfactometer bioassay, at a late stage of infection...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733293/androgen-responsive-non-coding-small-rnas-extend-the-potential-use-of-hcg-stimulation-as-a-bioassay-of-androgen-sufficiency
#17
Martina Rodie, Manikhandan Av Mudaliar, Pawel Herzyk, M McMillan, Massoud Boroujerdi, Sandra Chudleigh, Edward S Tobias, S Faisal Ahmed
BACKGROUND: It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC). AIMS & METHODS: To explore the effect of hCG-stimulation on the PBMC-transcriptome, 12 boys with a median age (range) of 0.7yrs (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation...
July 21, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28732295/determination-of-54-pesticides-in-waters-of-the-iberian-douro-river-estuary-and-risk-assessment-of-environmentally-relevant-mixtures-using-theoretical-approaches-and-artemia-salina-and-daphnia-magna-bioassays
#18
Catarina Cruzeiro, Sofia Amaral, Eduardo Rocha, Maria João Rocha
As a case study, the estuary of the international Douro River (Iberian Peninsula) was sampled over a year (2010) at six sampling sites to determine the presence of 56 pesticides of different categories (insecticides, herbicides, and fungicides). 96% of measured pesticides were detected in 79% of the quantified samples. Individual average pesticide concentrations ranged from 39 to 1 265ng/L, indicating a ubiquitous presence of the selected compounds; moreover, twelve pesticides were above the 2013/39/EU Directive limits...
July 18, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28732156/fixed-bed-modeling-of-nonsteroidal-anti-inflammatory-drug-removal-by-ion-exchange-in-synthetic-urine-mass-removal-or-toxicity-reduction
#19
Kelly Ann Landry, Treavor H Boyer
Ion-exchange removal of nonsteroidal anti-inflammatory drugs (NSAIDs) in synthetic urine can selectively remove pharmaceuticals with minimal co-removal of nutrients to enhance nutrient recovery efforts. However, the effect of endogenous metabolites in urine on ion-exchange removal, and the corresponding reduction in ecotoxicity potential of pharmaceuticals in treated urine entering the environment, is unknown. To assess treatment efficacy, this work paired predicted breakthrough curves determined by the homogenous surface diffusion model to an in vitro bioassay to evaluate COX-1 inhibition...
July 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28731697/total-synthesis-of-bruceolline-i
#20
Dina Scarpi, Cristina Faggi, Ernesto G Occhiato
The first total synthesis of the natural product bruceolline I, isolated in small quantities from the ethanol extract of Brucea mollis stems, was achieved in 29% yield over nine steps and with high enantiomeric purity (>98%). The key step of the process is the tandem gold-catalyzed rearrangement/Nazarov reaction of a propargylic acetate derivative. This synthesis provides a sufficient amount of synthesized bruceolline I for further bioassays.
July 21, 2017: Journal of Natural Products
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