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https://www.readbyqxmd.com/read/28635653/virtual-screening-against-phosphoglycerate-kinase-1-in-quest-of-novel-apoptosis-inhibitors
#1
Jie Xia, Bo Feng, Qianhang Shao, Yuhe Yuan, Xiang Simon Wang, Naihong Chen, Song Wu
Inhibition of apoptosis is a potential therapy to treat human diseases such as neurodegenerative disorders (e.g., Parkinson's disease), stroke, and sepsis. Due to the lack of druggable targets, it remains a major challenge to discover apoptosis inhibitors. The recent repositioning of a marketed drug (i.e., terazosin) as an anti-apoptotic agent uncovered a novel target (i.e., human phosphoglycerate kinase 1 (hPgk1)). In this study, we developed a virtual screening (VS) pipeline based on the X-ray structure of Pgk1/terazosin complex and applied it to a screening campaign for potential anti-apoptotic agents...
June 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28634478/transcriptional-and-antagonistic-responses-of-biocontrol-strain-lysobacter-enzymogenes-oh11-to-the-plant-pathogenic-oomycete-pythium-aphanidermatum
#2
Yangyang Zhao, Guoliang Qian, Yuan Chen, Liangcheng Du, Fengquan Liu
Lysobacter enzymogenes is a ubiquitous, beneficial, plant-associated bacterium emerging as a novel biological control agent. It has the potential to become a new source of antimicrobial secondary metabolites such as the Heat-Stable Antifungal Factor (HSAF), which is a broad-spectrum antimycotic with a novel mode of action. However, very little information about how L. enzymogenes detects and responds to fungi or oomycetes has been reported. An in vitro confrontation bioassay between the pathogenic oomycete Pythium aphanidermatum and the biocontrol bacterial strain L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28634053/using-short-term-bioassays-to-evaluate-the-endocrine-disrupting-capacity-of-the-pesticides-linuron-and-fenoxycarb
#3
Petra Spirhanzlova, Bert De Groef, Freda E Nicholson, Sylvia V H Grommen, Giulia Marras, Anthony Sébillot, Barbara A Demeneix, Sophie Pallud-Mothré, Gregory F Lemkine, Andrew J Tindall, David Du Pasquier
Several short-term whole-organism bioassays based on transgenic aquatic models are now under validation by the OECD (Organization for Economic Co-operation and Development) to become standardized test guidelines for the evaluation of the endocrine activity of substances. Evaluation of the endocrine disrupting capacity of pesticides will be a domain of applicability of these future reference tests. The herbicide linuron and the insecticide fenoxycarb are two chemicals commonly used in agricultural practices...
June 17, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28633389/multiple-soluble-tgf-%C3%AE-receptors-in-addition-to-soluble-endoglin-are-elevated-in-preeclamptic-serum-and-they-synergistically-inhibit-tgf-%C3%AE-signalling
#4
Yao Wang, Qi Chen, Min Zhao, Kelly Walton, Craig Harrison, Guiying Nie
Context: Preeclampsia (PE) can be classified into early-onset (<34wk of gestation) and late-onset (>34wk) subtypes. Soluble endoglin, an auxiliary receptor for TGF-β ligands, is increased in PE circulation and believed to inhibit TGF-β action by sequestering the ligands. However, soluble endoglin, with a low affinity to TGF-β ligands, is demonstrated to have little effect by itself on TGF-β action. Objectives: We examined whether multiple soluble TGF-β receptors are elevated in PE circulation and whether they synergistically block TGF-β signalling...
June 14, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28633108/bioactivity-and-quantitative-analysis-of-isohexenylnaphthazarins-in-root-periderm-of-two-echium-spp-e-%C3%A2-plantagineum-and-e-%C3%A2-gaditanum
#5
Alexandra G Durán, M Teresa Gutiérrez, Carlos Rial, Ascensión Torres, Rosa M Varela, Manuel M Valdivia, José M G Molinillo, Dominik Skoneczny, Leslie A Weston, Francisco A Macías
Isohexenylnaphthazarins are commonly found in the root periderm of several Boraginaceous plants and are known for their broad range of biological activities. The work described herein concerns the biological activity of compounds from the roots of Echium plantagineum L. and Echium gaditanum Boiss (Boraginaceae) collected from field sites in southern Spain and Australia. Bioactivity was assessed using etiolated wheat coleoptile bioassay and in vitro growth inhibitory activity in HeLa and IGROV-1 cells. The quantification of four isohexenylnaphthazarins (shikonin/alkannin, deoxyshikonin/deoxyalkannin, acetylshikonin/acetylalkannin and dimethylacrylshikonin/dimethylacrylalkannin) was performed by LC-MS/MS using juglone as internal standard...
June 17, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28631341/evaluation-of-flubendiamide-induced-mitochondrial-dysfunction-and-metabolic-changes-in-helicoverpa-armigera-hubner
#6
Bharat Nareshkumar, Shaik Mohammad Akbar, Hari Chand Sharma, Senigala K Jayalakshmi, Kuruba Sreeramulu
Phthalic acid diamide insecticides are the most effective insecticides used against most of the lepidopteran pests including Helicoverpa armigera, a polyphagous pest posing threat to several crops worldwide. The present studies were undertaken to understand different target sites and their interaction with insect ryanodine receptors (RyR). Bioassays indicated that flubendiamide inhibited the larval growth in dose-dependent manner with LD50 value of 0.72 μM, and at 0.8 μM larval growth decreased by about 88%...
June 20, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28630299/bacteria-mediated-hypoxia-functions-as-a-signal-for-mosquito-development
#7
Kerri L Coon, Luca Valzania, David A McKinney, Kevin J Vogel, Mark R Brown, Michael R Strand
Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that several mosquito species, including Aedes aegypti, do not develop beyond the first instar when fed a nutritionally complete diet in the absence of a gut microbiota. In contrast, several species of bacteria, including Escherichia coli, rescue development of axenic larvae into adults. The molecular mechanisms underlying bacteria-dependent growth are unknown. Here, we designed a genetic screen around E...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28629538/bioassay-guided-isolation-of-saikosaponins-with-agonistic-activity-on-5-hydroxytryptamine-2c-receptor-from-bupleurum-chinense-and-their-potential-use-for-the-treatment-of-obesity
#8
Chang-Li Sun, Chang-An Geng, Xiao-Yan Huang, Yun-Bao Ma, Xiao-Hong Zheng, Tong-Hua Yang, Xing-Long Chen, Xiu-Juan Yin, Xue-Mei Zhang, Ji-Jun Chen
5-Hydroxytryptamine 2C (5-HT2C) receptor is one of the major targets of anti-obesity agents, due to its role in regulation of appetite. In the present study, the 70% EtOH extract of the roots of Bupleurum chinense was revealed to have agonistic activity on 5-HT2C receptor, and the subsequent bioassay-guided isolation led to identification of several saikosaponins as the active constituents with 5-HT2C receptor agonistic activity in vitro and anti-obesity activity in vivo. The new compound, 22-oxosaikosaponin d (1), was determined by extensive spectroscopic analyses (HR-ESI-MS, IR, and 1D and 2D NMR)...
June 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28629112/european-demonstration-program-on-the-effect-based-and-chemical-identification-and-monitoring-of-organic-pollutants-in-european-surface-waters
#9
Zuzana Tousova, Peter Oswald, Jaroslav Slobodnik, Ludek Blaha, Melis Muz, Meng Hu, Werner Brack, Martin Krauss, Carolina Di Paolo, Zsolt Tarcai, Thomas-Benjamin Seiler, Henner Hollert, Sanja Koprivica, Marijan Ahel, Jennifer E Schollée, Juliane Hollender, Marc J-F Suter, Anita O Hidasi, Kristin Schirmer, Manoj Sonavane, Selim Ait-Aissa, Nicolas Creusot, Francois Brion, Jean Froment, Ana Catarina Almeida, Kevin Thomas, Knut Erik Tollefsen, Sara Tufi, Xiyu Ouyang, Pim Leonards, Marja Lamoree, Victoria Osorio Torrens, Annemieke Kolkman, Merijn Schriks, Petra Spirhanzlova, Andrew Tindall, Tobias Schulze
Growing concern about the adverse environmental and human health effects of a wide range of micropollutants requires the development of novel tools and approaches to enable holistic monitoring of their occurrence, fate and effects in the aquatic environment. A European-wide demonstration program (EDP) for effect-based monitoring of micropollutants in surface waters was carried out within the Marie Curie Initial Training Network EDA-EMERGE. The main objectives of the EDP were to apply a simplified protocol for effect-directed analysis, to link biological effects to target compounds and to estimate their risk to aquatic biota...
June 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28628716/imprinted-photonic-hydrogels-for-the-size-and-shell-selective-recognition-of-nanoparticles
#10
Sarra Gam-Derouich, Céline Bourdillon, Soraya Lakhdar Chaouche, Laurent Coolen, Agnès Maître, Claire Mangeney, Catherine Schwob
Sensors based on responsive photonic hydrogels have recently attracted considerable attention for visual medical diagnostics, pharmaceutical bioassays and environmental monitoring. However, the use of these promising materials for the detection of nanoparticles (NPs) has never been explored so far, although the sensing of nano-objects is a rapidly-evolving new area of research. To address this issue, we have combined the concepts of inverse opal hydrogels and nanoparticle-imprinted polymers. By this way, we could obtain a NP-imprinted photonic hydrogel, consisting in a three-dimensional, highly-ordered poly(methacrylic acid) macroporous array, where nanocavities complementary to the target NPs, here colloidal quantum dots, are distributed...
June 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28628635/cross-resistance-and-synergism-bioassays-suggest-multiple-mechanisms-of-pyrethroid-resistance-in-western-corn-rootworm-populations
#11
Adriano E Pereira, Dariane Souza, Sarah N Zukoff, Lance J Meinke, Blair D Siegfried
Recently, resistance to the pyrethroid bifenthrin was detected and confirmed in field populations of western corn rootworm, Diabrotica virgifera virgifera LeConte from southwestern areas of Nebraska and Kansas. As a first step to understand potential mechanisms of resistance, the objectives of this study were i) to assess adult mortality at diagnostic concentration-LC99 to the pyrethroids bifenthrin and tefluthrin as well as DDT, ii) estimate adult and larval susceptibility to the same compounds as well as the organophosphate methyl-parathion, and iii) perform synergism experiments with piperonyl butoxide (PBO) (P450 inhibitor) and S,S,S-tributyl-phosphorotrithioate (DEF) (esterase inhibitor) in field populations...
2017: PloS One
https://www.readbyqxmd.com/read/28627431/evaluation-of-the-quantamatrix-multiplexed-assay-platform-system-for-simultaneous-detection-of-mycobacteirum-tuberculosis-and-rifampicin-resistance-gene-using-culture-positive-mycobacteria
#12
Hye-Young Wang, Young Uh, Seoyong Kim, Tae-Sun Shim, Hyeyoung Lee
BACKGROUND: The differentiation of Mycobacterium tuberculosis complex (MTBC) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of anti-tuberculosis drugs. Prevailing data on rifampicin (RIF)-resistant TB is essential for early management of multidrug resistant-TB. The aim of this study was to evaluate the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform; QMAP) for the rapid differentiation of 23 Mycobacterium species including MTBC and RIF-resistant strains...
June 13, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28627124/cross-resistance-to-purified-bt-proteins-bt-corn-and-bt-cotton-in-a-cry2ab2-corn-resistant-strain-of-spodoptera-frugiperda
#13
Fei Yang, David L Kerns, Graham P Head, Paula Price, Fangneng Huang
BACKGROUND: Gene-pyramiding by combining two or more dissimilar Bt proteins into a crop has been used to delay insect resistance. The durability of gene-pyramiding can be reduced by cross-resistance. Fall armyworm, Spodoptera frugiperda, is a major target pest of the Cry2Ab2 protein used in pyramided Bt corn and cotton. Here, we provide the first experimental evaluation of cross-resistance in S. frugiperda selected with Cry2Ab2 corn to multiple Bt sources including purified Bt proteins, Bt corn and Bt cotton...
June 19, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28626571/integrative-approach-for-the-analysis-of-the-proteome-wide-response-to-bismuth-drugs-in-helicobacter-pylori
#14
Yuchuan Wang, Ligang Hu, Feng Xu, Quan Quan, Yau-Tsz Lai, Wei Xia, Ya Yang, Yuen-Yan Chang, Xinming Yang, Zhifang Chai, Junwen Wang, Ivan K Chu, Hongyan Li, Hongzhe Sun
Bismuth drugs, despite being clinically used for decades, surprisingly remain in use and effective for the treatment of Helicobacter pylori infection, even for resistant strains when co-administrated with antibiotics. However, the molecular mechanisms underlying the clinically sustained susceptibility of H. pylori to bismuth drugs remain elusive. Herein, we report that integration of in-house metalloproteomics and quantitative proteomics allows comprehensive uncovering of the bismuth-associated proteomes, including 63 bismuth-binding and 119 bismuth-regulated proteins from Helicobacter pylori, with over 60% being annotated with catalytic functions...
June 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28626483/bioassay-directed-isolation-of-falcarindiol-and-isoacetovanillon-from-pycnocycla-caespitosa-based-on-kcl-induced-contraction-in-rat-uterus-smooth-muscles
#15
Mostafa Ghanadian, Hassan Sadraei, Gholamreza Asghari, Zinat Abbasi
Hydroalcoholic extract and essential oil of aerial parts of Pycnocycla caespitosa have spasmolytic activity on rat ileum contractions. The objective of this research was to separate fractions of total hydroalcoholic extract of P. caespitosa guided by their spasmolytic activity on rat uterus. Aerial parts of P. caespitosa were extracted with ethanol. The concentrated extract was subjected to column chromatography and thin layer chromatography (TLC) for isolation fractions, then one of the bioactive fractions was subjected to further isolation to find its active components...
June 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28625913/use-of-rodent-data-for-cancer-risk-assessment-of-smokeless-tobacco-in-the-regulatory-context
#16
Robert Nilsson
To support risk management decisions, information from different fields has been integrated in this presentation to provide a realistic quantitative cancer risk assessment of smokeless tobacco. Smoking among Swedish men is currently below 10%, while about 20% use a special smokeless tobacco (snus) as a substitute for cigarettes. Epidemiological data and molecular biomarkers demonstrate that rodent bioassays with tobacco specific nitrosamines (TSNA) overestimate cancer risk from snus by more than one order of magnitude...
June 15, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28625782/complex-sexual-deception-in-an-orchid-is-achieved-by-co-opting-two-independent-biosynthetic-pathways-for-pollinator-attraction
#17
Haiyang Xu, Björn Bohman, Darren C J Wong, Claudia Rodriguez-Delgado, Adrian Scaffidi, Gavin R Flematti, Ryan D Phillips, Eran Pichersky, Rod Peakall
Sexually deceptive orchids lure their specific male pollinators using volatile semiochemicals that mimic female sex pheromones. To date, the semiochemicals known to be involved consist of blends of chemically and biosynthetically related compounds. In contrast, we report that (S)-β-citronellol and 2-hydroxy-6-methylacetophenone, two biosynthetically distinct compounds, are the active semiochemicals in Caladenia plicata, which is pollinated by male Zeleboria sp. thynnine wasps. They are also sex pheromone components of the female Zeleboria...
June 12, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28624777/a-novel-lack-of-fit-assessment-as-a-system-suitability-test-for-potency-assays
#18
Ruojia Li, Weiguo Cai, Marcel Zocher
Bioassay data analysis is used to determine the potency of protein therapeutics. To properly determine potency, the experimental data need to be fitted to a model that adequately describes the observed dose-response relationship. Typical models include 4-parameter logistic curve fits (Healy (5)), 5-parameter logistic curve fits (Prentice (6)) or parallel line analysis (Finney (7)). Lack-of-Fit (LOF) assessment can be used as a measure of potency assay system suitability to ensure appropriate closeness of the chosen model fit to the experimental data...
June 16, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28623798/monitoring-the-ecotoxicity-of-%C3%AE-al2o3-and-ni-%C3%AE-al2o3-nanomaterials-by-means-of-a-battery-of-bioassays
#19
Gabriela Svartz, Mariana Papa, Marina Gosatti, Marianela Jordán, Analia Soldati, Paula Samter, María M Guraya, Cristina Pérez Coll, Soledad Perez Catán
The increasing application of nanoparticles (NPs) to a variety of new technologies has become a matter of concern due to the potential toxicity of these materials. Many questions about the fate of NPs in the environment and the subsequent impact on ecosystems need to be answered. The aim of this work was to evaluate the ecotoxicity of two alumina-based nanoceramics, γ-Al2O3 (NC) and Ni/ γ-Al2O3 (NiNC) by means of three different standardized tests: Biochemical Oxygen Demand (BOD5), bioassay with luminescent bacteria (Vibrio fischeri; Microtox), and bioassay on amphibian larvae (Rhinella arenarum) (AMPHITOX)...
June 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28623487/multiple-receptor-ligand-based-pharmacophore-modeling-and-molecular-docking-to-screen-the-selective-inhibitors-of-matrix-metalloproteinase-9-from-natural-products
#20
Qi Gao, Yijun Wang, Jiaying Hou, Qizheng Yao, Ji Zhang
Matrix metalloproteinase-9 (MMP-9) is an attractive target for cancer therapy. In this study, the pharmacophore model of MMP-9 inhibitors is built based on the experimental binding structures of multiple receptor-ligand complexes. It is found that the pharmacophore model consists of six chemical features, including two hydrogen bond acceptors, one hydrogen bond donor, one ring aromatic regions, and two hydrophobic (HY) features. Among them, the two HY features are especially important because they can enter the S1' pocket of MMP-9 which determines the selectivity of MMP-9 inhibitors...
June 16, 2017: Journal of Computer-aided Molecular Design
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