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mitochondrial copy number hepatitis

Liwen Hu, Xinyue Yao, Yi Shen
Accumulating epidemiological evidence indicates that the quantitative changes in human mitochondrial DNA (mtDNA) copy number could affect the genetic susceptibility of malignancies in a tumor-specific manner, but the results are still elusive. To provide a more precise estimation on the association between mtDNA copy number and risk of diverse malignancies, a meta-analysis was conducted by calculating the pooled odds ratios (OR) and the 95% confidence intervals (95% CI). A total of 36 case-control studies involving 11,847 cases and 15,438 controls were finally included in the meta-analysis...
October 24, 2016: Scientific Reports
Runsheng Li, Yimin Jia, Shifeng Pan, Xian Li, Haogang Song, Ruqian Zhao
Obesity is associated with hepatic mitochondrial dysfunction. The relationship between glucocorticoids (GCs) and obesity has also been demonstrated in several researches. Recent research showed that GCs can affect the mitochondrial function. However, the role of glucocorticoid receptor (GR) in obesity-induced mitochondrial dysfunction remains unclear. C57BL/6 male mice fed with a high-fat diet (HFD) for 7 weeks were used as a model. The mice were shown to be overweight, together with lower serum and hepatic corticosterone levels...
February 2016: DNA and Cell Biology
Doaa I Hashad, Amany S Elyamany, Perihan E Salem
AIM: To assess the use of mitochondrial DNA (mtDNA) content as a noninvasive molecular biomarker in hepatitis C virus-related hepatocellular carcinoma (HCV-HCC). MATERIALS AND METHODS: A total of 135 participants were enrolled in the study. Equal numbers of subjects were enrolled in each of three clinically defined groups: those with HCV-related cirrhosis (HCV-cirrhosis), those with HCV-HCC, and a control group of age- and sex-matched healthy volunteers with no evidence of liver disease...
November 2015: Genetic Testing and Molecular Biomarkers
Hung-Wen Liu, Yin-Ching Chan, Ming-Fu Wang, Chu-Chun Wei, Sue-Joan Chang
Aging is accompanied by pathophysiological changes including insulin resistance and fatty liver. Dietary supplementation with (-)-epigallocatechin-3-gallate (EGCG) improves insulin sensitivity and attenuates fatty liver disease. We hypothesized that EGCG could effectively modulate aging-associated changes in glucose and lipid metabolism in senescence-accelerated mice (SAM) prone 8 (SAMP8). Higher levels of glucose, insulin, and free fatty acid, inhibited Akt activity, and decreased glucose transporter 4 (GLUT4) expression were observed in SAMP8 mice compared to the normal aging group, SAM resistant 1 mice...
September 30, 2015: Journal of Agricultural and Food Chemistry
A-Mei Zhang, Ke Ma, Yuzhu Song, Yue Feng, Haiping Duan, Ping Zhao, Binghui Wang, Gang Xu, Zheng Li, Xueshan Xia
Hepatitis C was the most popular chronic infectious liver disease worldwide. It was identified that Hepatitis C virus (HCV) infection could lead to mitochondrial dysfunction, though the mechanism was not fully understood. To investigate whether mtDNA copy number could be affected by HCV infection and be associated with clinical features of HCV patients, mtDNA copy numbers were analyzed in 242 patients with HCV infection and 226 matched control samples. The results suggested that mtDNA copy numbers significantly decreased in HCV patients (68...
July 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Peiyong Jiang, Carol W M Chan, K C Allen Chan, Suk Hang Cheng, John Wong, Vincent Wai-Sun Wong, Grace L H Wong, Stephen L Chan, Tony S K Mok, Henry L Y Chan, Paul B S Lai, Rossa W K Chiu, Y M Dennis Lo
The analysis of tumor-derived circulating cell-free DNA opens up new possibilities for performing liquid biopsies for the assessment of solid tumors. Although its clinical potential has been increasingly recognized, many aspects of the biological characteristics of tumor-derived cell-free DNA remain unclear. With respect to the size profile of such plasma DNA molecules, a number of studies reported the finding of increased integrity of tumor-derived plasma DNA, whereas others found evidence to suggest that plasma DNA molecules released by tumors might be shorter...
March 17, 2015: Proceedings of the National Academy of Sciences of the United States of America
J Wang, H J Zhang, K G Samuel, C Long, S G Wu, H Y Yue, L L Sun, G H Qi
The potential benefits of supplementing pyrroloquinoline quinone disodium (PQQ·Na2) in the diet of broiler chicks were explored. We first examined the effect of different levels of dietary PQQ·Na2 on growth performance, carcass characteristics, and plasma biochemical parameters (trial 1). A total of 490 1-day-old male Arbor Acres (AA) broiler chicks were randomly divided into 5 dietary groups supplemented with 0, 0.05, 0.1, 0.2, or 0.4 mg PQQ·Na2/kg feed. As the 0.2 mg/kg PQQ·Na2 supplement gave the best performance, we then investigated whether this level of PQQ·Na2 influenced the redox status of plasma samples and mitochondrial-related metabolism (trial 2)...
February 2015: Poultry Science
Ting Fu, Sunmi Seok, Sunge Choi, Zhang Huang, Kelly Suino-Powell, H Eric Xu, Byron Kemper, Jongsook Kim Kemper
Brown fat generates heat through uncoupled respiration, protecting against hypothermia and obesity. Adult humans have brown fat, but the amounts and activities are substantially decreased in obesity, by unknown mechanisms. Here we show that elevated microRNA 34a (miR-34a) in obesity inhibits fat browning in part by suppressing the browning activators fibroblast growth factor 21 (FGF21) and SIRT1. Lentivirus-mediated downregulation of miR-34a in mice with diet-induced obesity reduced adiposity, improved serum profiles, increased the mitochondrial DNA copy number, and increased oxidative function in adipose tissue in both BALB/c and C57BL/6 mice...
November 15, 2014: Molecular and Cellular Biology
Chun Tang, Hongming Liu, Yongliang Tang, Yong Guo, Xianchun Liang, Liping Guo, Ruxian Pi, Juntao Yang
Genetic susceptibility to alcoholic cirrhosis (AC) exists. We previously demonstrated hepatic mitochondrial DNA (mtDNA) damage in patients with AC compared with chronic alcoholics without cirrhosis. Mitochondrial transcription factor A (mtTFA) is central to mtDNA expression regulation and repair; however, it is unclear whether there are specific mtTFA single nucleotide polymorphisms (SNPs) in patients with AC and whether they affect mtDNA repair. In the present study, we screened mtTFA SNPs in patients with AC and analyzed their impact on the copy number of mtDNA in AC...
January 2014: Experimental and Therapeutic Medicine
Anne Davit-Spraul, Marine Beinat, Dominique Debray, Agnes Rötig, Abdelhamid Slama, Emmanuel Jacquemin
Multiple respiratory chain deficiencies represent a common cause of mitochondrial diseases and often result in hepatic failure. There is no gold-standard test for diagnosing mitochondrial disease, and the current diagnosis relies on establishing a consistent pattern of evidence from clinical data, neuroimaging, tissue biopsy, and biochemical investigations. In some patients, the mitochondrial respiratory chain defect (MRCD) diagnosis is confirmed by genetic investigations. In most cases, genetic investigations are not informative and a number of cases remain unexplained...
2014: JIMD Reports
Yimin Jia, Runsheng Li, Rihua Cong, Xiaojing Yang, Qinwei Sun, Nahid Parvizi, Ruqian Zhao
Mitochondrial oxidative phosphorylation (OXPHOS) plays an important role in energy homeostasis by controlling electron transfer and ATP generation. Maternal malnutrition during pregnancy affects mitochondrial (mt) DNA-encoded OXPHOS activity in offspring, yet it is unknown whether epigenetic mechanism is involved in the transcriptional regulation of mtDNA-encoded OXPHOS genes. In this study, 14 primiparous purebred Meishan sows were fed either standard- (SP, 12% crude protein) or low-protein (LP; 6% crude protein) diets throughout gestation, and the hepatic expression and transcriptional regulation of mtDNA-encoded OXPHOS genes were analyzed in newborn piglets...
2013: PloS One
Runsheng Li, Huafeng Zou, Yimin Jia, Ruqian Zhao
BACKGROUND: Mitochondria, which are essential for the functionality of eukaryotic cells, are particularly important in metabolically active tissues such as liver. Different breeds of pigs demonstrate distinct metabolic profiles in the liver, yet little is known whether the expression and transcriptional regulation of mitochondrial genes differ between breeds. RESULTS: Here we used male newborn Large White (LW) and Erhualian (EHL) piglets to delineate the difference in hepatic mitochondrial gene regulation between breeds...
2013: BMC Veterinary Research
Norihiro Kubota, Shoichi Kado, Mitsuyoshi Kano, Norie Masuoka, Yuriko Nagata, Toshihide Kobayashi, Kouji Miyazaki, Fumiyasu Ishikawa
The aim of the present study was to establish a progressive steatohepatitis mouse model because few reported animal models of non-alcoholic steatohepatitis (NASH) show the progression from fatty liver to steatohepatitis. C57BL/6N mice were fed a high-fat diet (HFD) to develop obesity and were either administered carbon tetrachloride (CCl4 ) eight times (0.05 mL/kg, s.c., once, followed by 0.1 mL/kg, s.c., seven times) or not. Serum parameters and hepatic histopathology were examined. In a separate experiment, CCl4 was administered subcutaneously from 0 to eight times to HFD-fed obese mice to investigate progressive changes...
July 2013: Clinical and Experimental Pharmacology & Physiology
Dong Chan Kim, Dae Won Jun, Eun Chul Jang, Sang Heum Kim, Eun Kyung Kim, Sang Pyo Lee, Kang Nyeong Lee, Hang Lak Lee, Oh Young Lee, Byung Chul Yoon, Ho Soon Choi
BACKGROUND/AIMS: It is suggested that the hepatic lipid composition is more important than lipid quantity in the pathogenesis of non-alcoholic steatohepatitis. We examined whether lipoic acid (LA) could alter intrahepatic lipid composition and free cholesterol distribution. METHODS: HepG2 cells were cultured with palmitic acid (PA) with and without LA. Apoptosis, changes of the mitochondrial structure, intracellular lipid partitioning, and reactive oxygen species (ROS) activity were measured...
March 2013: Gut and Liver
Hsu-Heng Yen, Kai-Lun Shih, Ta-Tsung Lin, Wei-Wen Su, Maw-Soan Soon, Chin-San Liu
AIM: To determine whether alteration of the mitochondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA(∆CT)) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)-infected patients. METHODS: This study enrolled two groups of patients aged 40-60 years: a control group and an HCV-infected group in Department of Gastroenterology and Hepatology in Changhua Christian Hospital. Patients with co-infection with hepatitis B virus or human immunodeficiency virus, autoimmune disease, malignant neoplasia, pregnancy, thyroid disease, or alcohol consumption > 40 g/d were excluded...
September 28, 2012: World Journal of Gastroenterology: WJG
Chun Tang, Heng Lin, Chun-Lin Feng, Qiao Wu, Yu-Jun Zhang, Ping Bie
This study investigated mitochondrial DNA (mtDNA) damage in rats with obstructive jaundice (OJ) and to explore its effect on mitochondrial and hepatic function. Forty-eight male Wistar rats were randomly divided into two groups: sham-operated (Sham) and bile duct ligation (BDL). Blood and tissue samples were collected from the two groups on days 1, 4, 7 and 14 following surgery. Hepatic and mitochondrial function were measured. Long and accurate PCR, restriction enzyme digestion and gene sequencing were used to analyze the locations of mtDNA deletions...
December 2012: Molecular Medicine Reports
Chun Tang, Xianchun Liang, Hongming Liu, Liping Guo, Ruxian Pi, Juntao Yang
The purpose of this study was to investigate hepatic mitochondrial DNA (mtDNA) damage and changes in its encoded products in patients with alcoholic cirrhosis (AC) in order to understand disease pathogenesis. We enrolled 23 patients with AC, 26 alcoholics without cirrhosis, and 25 normal subjects in this study. Hepatic mtDNA deletions were positioned using a combination of long and accurate polymerase chain reaction (LA PCR) and gene sequencing. The mtDNA copy number was measured using real-time quantitative PCR...
2012: International Journal of Clinical and Experimental Medicine
Jong-Jen Kuo, Hen-Hong Chang, Tung-Hu Tsai, Tzung-Yan Lee
Insulin resistance occurs in almost all patients with non-alcoholic fatty liver disease (NAFLD), and mitochondrial dysfunction likely plays a pivotal role in the progression of fatty liver into non-alcoholic steatohepatitis (NASH). Curcumin is a compound derived from the spice turmeric, a spice that is a potent antioxidant, anti-carcinogenic, and anti-hepatotoxic agent. The aim of this study was to analyze the ability of curcumin to protect against the mitochondrial impairment induced by high free fatty acids (HFFAs) and to determine the underlying mechanism for this cytoprotection...
September 2012: International Journal of Molecular Medicine
Varsha G Desai, Taewon Lee, Carrie L Moland, William S Branham, Roberta A Mittelstaedt, Sherry M Lewis, Julian E A Leakey, James C Fuscoe
The effects of 12-week exposure to zidovudine (AZT) at 400, 500, and 600 mg/kg/d were examined on expression of 542 mitochondria-related genes and mitochondrial DNA (mtDNA) copy number in the liver of male and female B6C3F(1) mice to understand mitochondrial role in sex-related differences in development of lactic acidosis. Plasma lactate levels and hematologic parameters were also examined. Results indicated increased red blood cell (RBC) count in vehicle-treated controls, whereas a dose-related decline in the RBC count was noted in AZT-treated mice compared to the basal levels before treatments began...
2012: AIDS Research and Treatment
M Pilar Valdecantos, Patricia Pérez-Matute, Pedro González-Muniesa, Pedro L Prieto-Hontoria, María J Moreno-Aliaga, J Alfredo Martínez
Nonalcoholic steatosis is an important hepatic complication of obesity linked to mitochondrial dysfunction and insulin resistance. Furthermore, lipoic acid has been reported to have beneficial effects on mitochondrial function. In this study, we analyzed the potential protective effect of lipoic acid supplementation against the development of nonalcoholic steatosis associated with a long-term high-fat diet feeding and the potential mechanism of this effect. Wistar rats were fed on a standard diet (n=10), a high-fat diet (n=10) and a high-fat diet supplemented with lipoic acid (n=10)...
December 2012: Journal of Nutritional Biochemistry
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