keyword
MENU ▼
Read by QxMD icon Read
search

Human Oocyte

keyword
https://www.readbyqxmd.com/read/29774457/vitamin-d-as-a-follicular-marker-of-human-oocyte-quality-and-a-serum-marker-of-in-vitro-fertilization-outcome
#1
Przemysław Ciepiela, Antoni J Dulęba, Ewelina Kowaleczko, Kornel Chełstowski, Rafał Kurzawa
PURPOSE: This study investigated the relationship between the vitamin D [25(OH)D] level in individual follicles and oocyte developmental competence. METHODS: A prospective cohort study in a private infertility center. Infertile women (N = 198) scheduled for intracytoplasmic sperm injection (ICSI) and a single embryo transfer (SET) provided serum samples and 322 follicular fluid (FF) specimens, each from a single follicle on the day of oocyte retrieval. RESULTS: FFs corresponding to successfully fertilized oocytes (following ICSI) contained significantly lower 25(OH)D level compared with those that were not fertilized (28...
May 17, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29772211/synthesis-folding-structure-and-activity-of-a-predicted-peptide-from-the-sea-anemone-oulactis-sp-with-an-shkt-fold
#2
Bankala Krishnarjuna, Jessica Villegas-Moreno, Michela L Mitchell, Agota Csoti, Steve Peigneur, Carlos Amero, Michael W Pennington, Jan Tytgat, Gyorgy Panyi, Raymond S Norton
Sea anemone venom is rich in bioactive compounds, including peptides containing multiple disulfide bridges. In a transcriptomic study on Oulactis sp., we identified the putative 36-residue peptide, OspTx2b, which is an isoform of the KV channel blocker OspTx2a (Sunanda P et al. (2018) Identification, chemical synthesis, structure and function of a new KV 1 channel blocking peptide from Oulactis sp. Peptide Science, in press). As OspTx2b contains a ShK/BgK-like cysteine framework, with high amino acid sequence similarity to BgK, we were interested to investigate its structure and function...
May 14, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29772022/binding-of-sulphonylureas-to-plasma-proteins-a-katp-channel-perspective
#3
Peter Proks, Holger Kramer, Elizabeth Haythorne, Frances M Ashcroft
Sulphonylurea drugs stimulate insulin secretion from pancreatic β-cells primarily by inhibiting ATP sensitive potassium (KATP) channels in the β-cell membrane. The effective sulphonylurea concentration at its site of action is significantly attenuated by binding to serum albumin, which makes it difficult to compare in vitro and in vivo data. We therefore measured the ability of gliclazide and glibenclamide to inhibit KATP channels and stimulate insulin secretion in the presence of serum albumin. We used this data, together with estimates of free drug concentrations from binding studies, to predict the extent of sulphonylurea inhibition of KATP channels at therapeutic concentrations in vivo...
2018: PloS One
https://www.readbyqxmd.com/read/29755973/identification-of-loop-d-domain-amino-acids-in-the-human-aquaporin-1-channel-involved-in-activation-of-the-ionic-conductance-and-inhibition-by-aqb011
#4
Mohamad Kourghi, Michael L De Ieso, Saeed Nourmohammadi, Jinxin V Pei, Andrea J Yool
Aquaporins are integral proteins that facilitate the transmembrane transport of water and small solutes. In addition to enabling water flux, mammalian Aquaporin-1 (AQP1) channels activated by cyclic GMP can carry non-selective monovalent cation currents, selectively blocked by arylsulfonamide compounds AqB007 (IC50 170 μM) and AqB011 (IC50 14 μM). In silico models suggested that ligand docking might involve the cytoplasmic loop D (between AQP1 transmembrane domains 4 and 5), but the predicted site of interaction remained to be tested...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29753045/identification-and-functional-characterization-of-zebrafish-k-2p-17-1-task-4-talk-2-two-pore-domain-k-channels
#5
Ingo Staudacher, Claudius Illg, Jakob Gierten, Sebastian Seehausen, Patrick A Schweizer, Hugo A Katus, Dierk Thomas
Human K2P 17.1 (TASK-4, TALK-2) two-pore-domain potassium (K2P ) channels have recently been implicated in heart rhythm disorders including atrial fibrillation and conduction disease. The functional in vivo significance of K2P 17.1 currents in cardiac electrophysiology remains incompletely understood. Danio rerio (zebrafish) may be utilized to elucidate the role of cardiac K2P channels in vivo. The aim of this work was to identify and characterize the zebrafish ortholog of K2P 17.1 in comparison to its human counterpart...
May 9, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29751638/zika-virus-trafficking-and-interactions-in-the-human-male-reproductive-tract
#6
REVIEW
Lucia Regina Cangussu da Silva
Sexual transmission of Zika virus (ZIKV) is a matter of great concern. Infectious viral particles can be shed in semen for as long as six months after infection and can be transferred to male and female sexual partners during unprotected sexual intercourse. The virus can be found inside spermatozoa and could be directly transferred to the oocyte during fertilization. Sexual transmission of ZIKV can contribute to the rise in number of infected individuals in endemic areas as well as in countries where the mosquito vector does not thrive...
May 11, 2018: Pathogens
https://www.readbyqxmd.com/read/29750278/bmp6-down-regulates-gdnf-expression-through-smad1-5-and-erk1-2-signaling-pathways-in-human-granulosa-lutein-cells
#7
Xin-Yue Zhang, Hsun-Ming Chang, Elizabeth L Taylor, Peter C K Leung, Rui-Zhi Liu
Bone morphogenetic protein 6 (BMP6) is a critical regulator of follicular development that is expressed in mammalian oocytes and granulosa cells. Glial cell line-derived neurotrophic factor (GDNF) is an intraovarian neurotrophic factor that plays an essential role in regulating mammalian oocyte maturation. The aim of this study was to investigate the effect of BMP6 on the regulation of GDNF expression and the potential underlying mechanisms. We used an established immortalized human granulosa cell line (SVOG cells) and primary human granulosa-lutein cells as in vitro cell models...
May 9, 2018: Endocrinology
https://www.readbyqxmd.com/read/29747744/yokukansan-contains-compounds-that-antagonize-the-5-ht-3-receptor
#8
Yukiko Nakamura, Yusuke Ishida, Makoto Kondo, Shoichi Shimada
BACKGROUND: We recently focused on alkaloids in Uncaria hook (a constituent of the Kampo medicine, yokukansan) and identified the pharmacological action of geissoschizine methyl ether on several G protein-coupled receptors. However, the functions of other identified alkaloids in Uncaria hook, including hirsutine, hirsuteine, rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine, are not clear. PURPOSE: To evaluate the effect of seven alkaloids in Uncaria hook (hirsutine, hirsuteine, rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine and geissoschizine methyl ether) on the hydroxytryptamine type-3 (5-HT3 ) receptor ion channel...
April 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29746897/the-role-of-ca-2-in-oocyte-activation-during-in-vitro-fertilization-insights-into-potential-therapies-for-rescuing-failed-fertilization
#9
REVIEW
Karl Swann
At fertilization the mature mammalian oocyte is activated to begin development by a sperm-induced series of increases in the cytosolic free Ca2+ concentration. These so called Ca2+ oscillations, or repetitive Ca2+ , are also seen after intracytoplasmic sperm injection (ICSI) and are primarily triggered by a sperm protein called phospholipase Czeta (PLCζ). Whilst ICSI is generally an effective way to fertilizing human oocytes, there are cases where oocyte activation fails to occur after sperm injection. Many such cases appear to be associated with a PLCζ deficiency...
May 7, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29741247/prenatal-and-preimplantation-diagnosis-of-hemoglobinopathies
#10
REVIEW
C Vrettou, G Kakourou, T Mamas, J Traeger-Synodinos
The hemoglobinopathies, as a group, are one of the most common serious monogenic diseases in the world. An accepted and widely adopted approach to reduce the number of new cases involves carrier-screening programs, with the option of prenatal diagnosis (PND) or preimplantation diagnosis (preimplantation genetic testing for monogenic disease, PGT-M) for carrier couples. The aim of PND is to provide an accurate result as early in pregnancy as possible, which necessitates prior identification of the parental disease-causing mutations, as well as safe and timely biopsy of fetal material...
May 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29739821/c-type-natriuretic-peptide-a-link-between-hyperandrogenism-and-anovulation-in-a-mouse-model-of-polycystic-ovary-syndrome
#11
Adelina M Reis, Kinulpe Honorato-Sampaio
The polycystic ovary (PCO) syndrome (PCOS) is the most common cause of anovulatory infertility in women and is associated with several clinical disorders. Despite the great amount of research in the area, mechanisms involved in the genesis of this syndrome remain poorly understood. In a recent issue of Clinical Science (vol. 132, issue 7, 759-776), Wang and colleagues, highlight the important role of overactivated C-type natriuretic peptide (CNP) and natriuretic peptide receptor 2 (CNP/NPR2) system in preventing oocyte maturation and ovulation in PCOS mice model induced by androgen...
May 16, 2018: Clinical Science (1979-)
https://www.readbyqxmd.com/read/29734441/vitrfication-and-slow-freezing-for-cryopreservation-of-germinal-vesicle-stage-human-oocytes-a-bayesian-meta-analysis
#12
H S Youm, J R Choi, D Oh, Y H Rho
BACKGROUND: T he most commonly used methods for the cryopreservation of oocytes and embryos are vitrification and slow freezing. OBJECTIVE: The aim of this study was to to investigate whether there are differences in survival, in vitro maturation (IVM), and fertilization rates between cryopreserved immature oocytes, especially germinal vesicle (GV)-stage human oocytes, following vitrification and slow freezing. MATERIALS AND METHODS: A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases...
November 2017: Cryo Letters
https://www.readbyqxmd.com/read/29734424/learning-aspects-in-implementing-vitrification-program-using-mouse-blastocysts
#13
E J Hoekman, K E Tucker, C Am Jansen, C G Hilders
BACKGROUND: Oocyte vitrification is important for fertility preservation. There are debates, however, surrounding the components of the procedure itself. OBJECTIVE: before starting a vitrification program, we decided to (1) determine if blastocysts derived from previously frozen mouse embryos would be suitable to practice vitrification and (2) to analyze the factors that contributed to improving our "learning curve". MATERIALS AND METHODS: 58 expanded blastocysts cultured from commercially available frozen 1-cell mouse embryos (B6D2F1 x B6C3F1) were used...
March 2018: Cryo Letters
https://www.readbyqxmd.com/read/29731504/application-of-auxin-inducible-degron-technology-to-mouse-oocyte-activation-with-plc%C3%AE
#14
Kento Miura, Shogo Matoba, Narumi Ogonuki, Takafumi Namiki, Junya Ito, Naomi Kashiwazaki, Atsuo Ogura
In mammals, spermatozoa activate oocytes by triggering a series of intracellular Ca2+ oscillations with phospholipase C zeta (PLCζ), a sperm-borne oocyte-activating factor. Because the introduction of PLCζ alone can induce oocyte activation, it might be a promising reagent for assisted reproductive technologies. To test this possibility, we injected human PLCζ (hPLCζ) mRNA into mouse oocytes at different concentrations. We observed the oocyte activation and subsequent embryonic development. Efficient oocyte activation and embryonic development to the blastocyst stage was achieved only with a limited range of mRNA concentrations (0...
May 5, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29727537/-testicular-histology-does-not-affect-the-clinical-outcomes-of-icsi-in-men-with-non-obstructive-azoospermia
#15
Yan-Rong Kuai, Zhan-Ju He, Sheng Wang, Kai Zhang, Cheng Zeng, Liang Chen, Qing Xue, Jing Shang, Hui-Xia Yang, Yang Xu
Objective: To investigate whether testicular histology influences the clinical outcomes of intracytoplasmic sperm injection (ICSI) in men with non-obstructive azoospermia (NOA). METHODS: We retrospectively analyzed the clinical data about 73 cases of NOA undergoing ICSI, including 105 ICSI cycles and 79 embryo transfer cycles. The infertility of the patients was attributed to male factors only or both male and female tube factors and the females' age was ≤38 years...
October 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29720659/chromatin-analysis-in-human-early-development-reveals-epigenetic-transition-during-zga
#16
Jingyi Wu, Jiawei Xu, Bofeng Liu, Guidong Yao, Peizhe Wang, Zili Lin, Bo Huang, Xuepeng Wang, Tong Li, Senlin Shi, Nan Zhang, Fuyu Duan, Jia Ming, Xiangyang Zhang, Wenbin Niu, Wenyan Song, Haixia Jin, Yihong Guo, Shanjun Dai, Linli Hu, Lanlan Fang, Qiujun Wang, Yuanyuan Li, Wei Li, Jie Na, Wei Xie, Yingpu Sun
Upon fertilization, drastic chromatin reorganization occurs during preimplantation development 1 . However, the global chromatin landscape and its molecular dynamics in this period remain largely unexplored in humans. Here we investigate chromatin states in human preimplantation development using an improved assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) 2 . We find widespread accessible chromatin regions in early human embryos that overlap extensively with putative cis-regulatory sequences and transposable elements...
May 2, 2018: Nature
https://www.readbyqxmd.com/read/29718716/complex-chromosomal-aberrations-in-a-fetus-originating-from-oocytes-with-smooth-endoplasmic-reticulum-ser-aggregates
#17
Ioannis A Sfontouris, George T Lainas, Tryfon G Lainas, Efthimios Faros, Maria Banti, Katerina Kardara, Katerina Anagnostopoulou, Harris Kontos, George K Petsas, Efstratios M Kolibianakis
The presence of smooth endoplasmic reticulum aggregates (SERa) in the ooplasm is considered as the most severe oocyte dysmorphism due to its serious and potentially lethal outcomes in offspring. In the present case report, a couple underwent their first intracytoplasmic sperm injection (ICSI) cycle using a gonadotrophin releasing hormone (GnRH) antagonist protocol, followed by fetal ultrasound scanning and amniocentesis. SERa were observed in all oocytes retrieved. A singleton pregnancy was established. The second trimester fetal ultrasound scan revealed a female fetus with overlapping fingers in both hands, and amniocentesis was performed for the detection of chromosomal abnormalities...
May 2, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29715701/application-of-stem-cells-to-infertility-treatment-with-emphasis-on-mesenchymal-stem-cells-and-ovarian-stem-cells
#18
Nahid Lorzadeh, Nastaran Kazemirad
Stem cells are a population of undifferentiated cells that originated from embryonic and fetal stages of development. Stem cells possess the potency to produce cells that ultimately form different tissues and organs. They are considered as potential alternative novel therapeutic agents for patients suffering from infertility due to their unlimited source and high differentiation potency. There are several studies that have shown that different types of stem cells (mesenchymal stem cells, embryonic, endometrial, and ovarian stem cells) can be differentiated into both spermatozoa and oocytes in vitro, proving their potential clinical use in management and cure of infertility issues...
May 1, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29709724/despite-the-donor-s-age-human-adipose-derived-stem-cells-enhance-the-maturation-and-development-rates-of-porcine-oocytes-in-a-co-culture-system
#19
REVIEW
Erif Maha Nugraha Setyawan, Hyun Ju Oh, Min Jung Kim, Geon A Kim, Seok Hee Lee, Yoo Bin Choi, Kihae Ra, Byeong Chun Lee
The paracrine interactions between cumulus-oocyte complexes (COCs) and follicular somatic cells during in vitro maturation (IVM) were investigated. To optimize IVM conditions, many studies have applied exogenous growth factors and cell feeding/co-culture systems using various cell types to replicate the natural follicular microenvironment during IVM. A potential candidate as cell feeders is adipose-derived stem cells (ASCs) which secrete high levels of growth factors that have roles in oocyte maturation. However, the cell donor's age should be considered because biological aging also occurs in stem cells...
December 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/29708548/using-mouse-oocytes-to-assess-human-gene-function-during-meiosis-i
#20
Diego Marin, Alexandra L Nguyen, Richard T Scott, Karen Schindler
Embryonic aneuploidy is the major genetic cause of infertility in humans. Most of these events originate during female meiosis, and albeit positively correlated with maternal age, age alone is not always predictive of the risk of generating an aneuploid embryo. Therefore, gene variants might account for incorrect chromosome segregation during oogenesis. Given that access to human oocytes is limited for research purposes, a series of assays were developed to study human gene function during meiosis I using mouse oocytes...
April 10, 2018: Journal of Visualized Experiments: JoVE
keyword
keyword
98110
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"