keyword
https://read.qxmd.com/read/38202845/gender-control-of-mouse-embryos-by-activation-of-tlr7-8-on-x-sperm-via-ligands-dsrna-40-and-dsrna-dr
#21
JOURNAL ARTICLE
Yunfei Hou, Jingfeng Peng, Linjun Hong, Zhenfang Wu, Enqin Zheng, Zicong Li
Gender control technologies are promising for enhancing the production efficiency of the farm animal industry, and preventing sex-linked hereditary diseases in humans. It has been shown that the X sperm of mammalian animals specifically expresses X-chromosome-derived toll-like receptor 7/8 (TLR7/8), and the activation of TLR7/8 on the X sperm by their agonist, R848, can separate X and Y sperm via the specific inhibition of X sperm motility. The use of R848-preselected sperm for fertilization resulted in sex-ratio-skewed embryos or offspring...
January 4, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38195558/the-morphokinetic-signature-of-human-blastocysts-with-mosaicism-and-the-clinical-outcomes-following-transfer-of-embryos-with-low-level-mosaicism
#22
JOURNAL ARTICLE
Yaoyu Zou, Yilun Sui, Jing Fu, Naidong Ge, Xiaoxi Sun, Yijuan Sun
BACKGROUND: Genetic mosaicism is commonly observed in human blastocysts. Embryos' morphokinetic feature observed from time-lapse monitoring (TLM) is helpful to predict the embryos' ploidy status in a non-invasive way. However, morphokinetic research on mosaic embryos is extremely limited. Moreover, transfer of mosaic embryos is a new attempt in reproductive medicine, while studies regarding the clinical and neonatal outcomes following transfer of embryos with different levels and types of mosaicism are needed...
January 9, 2024: Journal of Ovarian Research
https://read.qxmd.com/read/38191818/pre-fertilization-approach-using-%C3%AE-l-fucosidase-modulates-zona-pellucida-hardening-during-bovine-in-vitro-embryo-production
#23
JOURNAL ARTICLE
Thainá Sallum Bacco Manssur, Talita Raquel Cavichioli Sebastião, Fernanda Fagali Franchi, Priscila Helena Dos Santos, Eduardo Montanari Razza, Sarah Gomes Nunes, Anthony César de Souza Castilho, Patricia Kubo Fontes
The polyspermy occurrence is considerably lower under in vivo compared to in vitro embryo culture conditions, suggesting that the presence of some factors in the maternal environment is responsible for this. The α-L-fucosidase (FUCA) is a natural glycosidase present in the oviductal fluid, therefore, this study aimed at investigating the effect of adding FUCA to the hardening of the zona pellucida (ZP), polyspermy control, and embryonic yield and quality of bovine blastocysts produced in vitro. In the first experiment, the effect of FUCA (0...
January 8, 2024: Veterinary Research Communications
https://read.qxmd.com/read/38185698/association-of-early-cleavage-morula-compaction-and-blastocysts-ploidy-of-ivf-embryos-cultured-in-a-time-lapse-system-and-biopsied-for-genetic-test-for-aneuploidy
#24
JOURNAL ARTICLE
H De Martin, T C S Bonetti, C A Z Nissel, A P Gomes, M G Fujii, P A A Monteleone
IVF embryos have historically been evaluated by morphological characteristics. The time-lapse system (TLS) has become a promising tool, providing an uninterrupted evaluation of morphological and dynamic parameters of embryo development. Furthermore, TLS sheds light on unknown phenomena such as direct cleavage and incomplete morula compaction. We retrospectively analyzed the morphology (Gardner Score) and morphokinetics (KIDScore) of 835 blastocysts grown in a TLS incubator (Embryoscope+), which were biopsied for preimplantation genetic testing for aneuploidy (PGT-A)...
January 7, 2024: Scientific Reports
https://read.qxmd.com/read/38177973/what-is-the-maximal-timeframe-between-sperm-acquisition-to-sperm-cryopreservation-in-different-culture-conditions
#25
JOURNAL ARTICLE
Raoul Orvieto, Chen Shimon, Olga Dratviman-Storobinsky, Meirav Noach-Hirsh, Adva Aizer
OBJECTIVE: Most of the literature about postmortem sperm retrieval (PMSR) deals with the controversies surrounding ethical and legal aspects, while the optimal time interval between the death and viable sperm acquisition is indefinite. In an attempt to aid fertility specialists, while counseling whether to pursue and adopt PMSR, we aim to explore the maximal time frame from ejaculated sperm acquisition to sperm cryopreservation in different "culture" conditions, observations that might be extrapolated to PMSR requests...
January 5, 2024: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/38159580/effect-of-prewarmed-crystalloid-fluids-and-an-in-line-fluid-warming-device-on-fluid-outflow-temperatures-at-ambient-temperatures-of-21-22%C3%A2-c-and-3-5%C3%A2-c
#26
JOURNAL ARTICLE
Lindsay C Monroe, Jarrod R Troy, Clark Kogan, Jamie J Kopper
Equine veterinarians frequently treat patients in non-climate-controlled (i.e., hospitalized) settings. In colder environments, intravenous (IV) fluid administration can contribute to patient hypothermia. The objective of this study was to evaluate three IV fluid warming mechanisms to determine their effect on fluid outflow temperatures at ambient temperatures of 21-22°C and 3.5°C. In this study, fluid outflow temperatures were measured using three warming mechanisms: (1) pre-warmed fluids at 42°C, (2) an in-line warming device placed 163 cm away from the Luer-lock fluid line adaptor (location 1) on the fluid line, and (3) an in-line warming device placed 88 cm away from the Luer-lock fluid line adaptor (location 2) on the fluid line...
December 28, 2023: Journal of Equine Veterinary Science
https://read.qxmd.com/read/38107234/variations-in-metabolic-parameters-of-in-vitro-matured-porcine-oocytes-after-vitrification-warming
#27
JOURNAL ARTICLE
Sergio Morado, Ailén Aparicio, Daniela Pinchetti, Claudia Cecilia Arraztoa, Gabriel Alvarez, Cynthia Gutnisky, Deborah Neild, Gabriel Dalvit, Pablo Cetica
BACKGROUND: As the porcine oocyte is the most sensitive to low-temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species. AIM: Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. In addition, metaphase II plate recovery time analysis, in vitro fertilization, and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity...
November 2023: Open Veterinary Journal
https://read.qxmd.com/read/38103883/impact-of-oxygen-tension-during-in-vitro-maturation-a-sibling-oocyte-prospective-double-blinded-study
#28
JOURNAL ARTICLE
Yasmine Boumerdassi, Julie Labrosse, Fatma Hammami, Mehdi Dahoun, Jean Bouyer, Louis O'Neill, Solmaz Sarandi, Maeliss Peigné, Isabelle Cedrin, Michael Grynberg, Christophe Sifer
OBJECTIVE: To determine whether oxygen tension (20% versus 5%) has an impact on oocyte maturation rates and morphology during in vitro maturation. DESIGN: A prospective, observational, monocentric, sibling-oocyte study. SUBJECTS: A total of 143 patients who underwent in vitro maturation for fertility preservation purposes from November 2016 to April 2021 were analyzed. Patients were included when ≥ 2 cumulus-oocyte complexes were retrieved...
December 14, 2023: Fertility and Sterility
https://read.qxmd.com/read/38061461/progesterone-primed-cycles-result-in-slower-embryos-without-compromising-implantation-potential-and-with-the-advantages-of-oral-administration-and-potential-cost-reduction
#29
JOURNAL ARTICLE
Daniela Braga, Amanda Setti, Edward Carrilho, Patrícia Guilherme, Assumpto Iaconelli, Edson Borges
OBJECTIVE: To study the impact of the use of progesterone on embryo morphokinetics and on the outcomes of intracytoplasmic sperm injection (ICSI) cycles. DESIGN: Cohort study SETTING: Private university-affiliated in vitro fertilization center. PATIENTS: The study included 236 freeze-all ICSI cycles and the resultant 2,768 injected oocytes cultured in a time-lapse imaging (TLI) incubation system. Patients were matched by age and divided into groups depending on the protocol used to prevent the LH surge: progestin-primed (n=144 cycles and 1,360 embryos) and GnRH-antagonist group (n=144 cycles and 1,408 embryos)...
December 5, 2023: F&S science
https://read.qxmd.com/read/38051756/the-drosophila-drop-dead-gene-is-required-for-eggshell-integrity
#30
JOURNAL ARTICLE
Tayler D Sheahan, Amanpreet Grewal, Laura E Korthauer, Edward M Blumenthal
The eggshell of the fruit fly Drosophila melanogaster is a useful model for understanding the synthesis of a complex extracellular matrix. The eggshell is synthesized during mid-to-late oogenesis by the somatic follicle cells that surround the developing oocyte. We previously reported that female flies mutant for the gene drop-dead (drd) are sterile, but the underlying cause of the sterility remained unknown. In this study, we examined the role of drd in eggshell synthesis. We show that eggs laid by drd mutant females are fertilized but arrest early in embryogenesis, and that the innermost layer of the eggshell, the vitelline membrane, is abnormally permeable to dye in these eggs...
2023: PloS One
https://read.qxmd.com/read/38041463/carbon-monoxide-exposure-does-not-improve-the-in-vitro-fertilization-rate-of-oocytes-obtained-from-heterozygous-hmox1-knockout-mice
#31
JOURNAL ARTICLE
Federica Romanelli, Maria Laura Zenclussen, Ana Claudia Zenclussen, Nicole Meyer
In our experimental study we explored the impact of maternal reduced heme oxygenase-1 (HO-1) gene ( Hmox1 ) expression on the in vitro fertilization (IVF) rate through the use of heterozygous Hmox1 knockout mice models (HET/Hmox1+/ -). Also, we hypothesized a beneficial role of gametes exposure during fertilization to carbon monoxide (CO), one of HO-1 by-products, that might be relevant for the improvement of IVF rates. IVF technique was performed by using oocytes obtained from wild-type (WT) or Hmox1+ / - dams fertilized with WT, Hmox1+ / - or Hmox1- / - mice-derived sperm...
November 7, 2023: International Journal of Fertility & Sterility
https://read.qxmd.com/read/38019384/in-vitro-post-ovulatory-oocyte-ageing-in-grass-carp-ctenopharyngodon-idella-affects-h4k12-acetylation-pattern-and-histone-acetyltransferase-activity
#32
JOURNAL ARTICLE
Azin Mohagheghi Samarin, Azadeh Mohagheghi Samarin, Swapnil Gorakh Waghmare, Marianne Danielsen, Hanne Søndergård Møller, Tomáš Policar, Otomar Linhart, Trine Kastrup Dalsgaard
Delayed fertilization leads to the ageing of post-ovulatory oocytes and reduces the developmental competence of arising embryos. Little information is available about the molecular processes during fish oocyte ageing. The current study investigated the functional consequences of oocyte ageing in grass carp Ctenopharyngodon idella embryos. In addition, the dynamics of selected post-transcriptionally modified histones (acetylation of H3K9, H3K14, H4K5, H4K8, H4K12, and H4K16) were analyzed during oocyte ageing...
November 29, 2023: Fish Physiology and Biochemistry
https://read.qxmd.com/read/38013657/motility-oxidative-status-and-morphology-of-frozen-thawed-bovine-semen-are-not-impacted-by-fatty-acid-exposure-in%C3%A2-vitro
#33
JOURNAL ARTICLE
Camila Cupper Vieira, Daniele Missio, Daniela Dos Santos Brum, Rafaela Dalmolin Menezes, Francielli Weber Santos Cibin, Fernando Silveira Mesquita, Paulo Bayard Dias Gonçalves, Rogério Ferreira
While sperm migrate within the reproductive tract of cows experiencing negative energy balance (NEB), they come into contact with elevated concentrations of non-esterified fatty acids (NEFA). For this reason, this study aimed to investigate the effects of three different NEFA - palmitic acid (PA), stearic acid (SA), and oleic acid (OA) - on bovine sperm motility, kinetic parameters, oxidative status, and morphology. Frozen thawed semen samples from Bos taurus bulls were incubated with varying concentrations of each fatty acid, and the sperm's characteristics were analysed at different time points...
November 28, 2023: Reproduction in Domestic Animals
https://read.qxmd.com/read/37996572/diazotrophic-azotobacter-salinestris-yrnf3-a-probable-calcite-solubilizing-bio-agent-for-improving-the-calcareous-soil-properties
#34
JOURNAL ARTICLE
Younes M Rashad, Mohamed Hafez, Mohamed Rashad
Calcareous soils are characterized by a high calcium carbonate content (calcite), which plays a crucial role in the soil structure, plant growth, and nutrient availability. The high content of CaCO3 leads to the increment of the soil alkalinity, which results in a lowering of the nutrient availability causing a challenge for the agriculture in these soils. In this study, the calcite-solubilizing potential of the diazotrophic Azotobacter salinestris YRNF3 was investigated in vitro as a probable bio-agent for enhancing the calcareous soils properties such as soil pH and nutrient availability...
November 23, 2023: Scientific Reports
https://read.qxmd.com/read/37965035/simple-cryopreservation-protocol-for-luffa-pollen-enhancing-breeding-efficiency
#35
JOURNAL ARTICLE
Arvind Nagar, Ravi Gowthami, Amish Kumar Sureja, Anilabha Das Munshi, Manjusha Verma, Awani Kumar Singh, Niharika Mallick, Jogendra Singh, Subhash Chander, Muthusamy Shankar, Pooja Pathania, Subramani Rajkumar
This study aimed to develop a long-term pollen storage protocol for Luffa species ( L. acutangula , L. cylindrica , L. echinata , and L. graveolens ) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica , and L. echinata , and BK medium with 3% sucrose ideal for L. graveolens . Overestimation in staining tests compared to in vitro pollen germination was observed...
2023: Frontiers in Plant Science
https://read.qxmd.com/read/37964175/bicarbonate-and-bsa-increase-the-capacitation-pattern-and-acrosomal-exocytosis-in-boar-sperm-after-120-min-of-incubation
#36
JOURNAL ARTICLE
Sylwia Dudkiewicz, Patricia Peris-Frau, Helena Nieto-Cristóbal, Julián Santiago-Moreno, Eduardo de Mercado, Manuel Álvarez-Rodríguez
Sperm capacitation is a crucial step towards the acquisition of fertilizing capacity. Despite the attempts to mimic the in vivo situation, there is still a lack of standardization in vitro techniques. Bicarbonate and serum albumin (BSA) are routinely used, although controversial results are reported regarding the optimal concentration of each compound. In addition, whether caffeine is needed on in vitro capacitation media in boar sperm remains to be elucidated. Here, 18 boar commercial artificial insemination doses were used to test different concentrations of bicarbonate (19, 37 or 56 mM) in experiment 1, BSA (1...
November 14, 2023: Reproduction in Domestic Animals
https://read.qxmd.com/read/37956580/effects-of-nobiletin-supplementation-on-the-freezing-diluent-on-porcine-sperm-cryo-survival-and-subsequent-in-vitro-embryo-development
#37
JOURNAL ARTICLE
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Nobiletin (NOB) is a bioflavonoid compound isolated from citrus fruit peels. The present study aimed to elucidate whether NOB facilitates the porcine sperm cryosurvival and embryo development after in vitro fertilization (IVF). To this end, spermatozoa were diluted and cryopreserved in a freezing extender supplemented with 0 (control), 50, 100, 150, and 200 μM Nobiletin. The kinematic patterns of frozen-thawed (FT) sperm were assessed after 30 and 90 min incubation using a Sperm Class Analyzer (SCA)...
November 10, 2023: Theriogenology
https://read.qxmd.com/read/37951584/octreotide-improves-human-lymphatic-fluid-transport-a-translational-trial
#38
JOURNAL ARTICLE
Thomas Holm-Weber, Frederik Skov, Sheyanth Mohanakumar, Lene Thorup, Troels Riis, Mikkel Bring Christensen, David Peick Sonne, Per Bo Jensen, Donna Briggs Bødtkjer, Vibeke Elisabeth Hjortdal
OBJECTIVE: Chylothorax is a complex condition and many different pharmacological agents have been tried as treatment. Octreotide is used off-label to treat chylothorax, but the efficacy of octreotide remains unclear. A decrease in lymph production is suggested as the mechanism. In this cross-over study, we explore the direct effect of octreotide on human lymphatic drainage. METHODS: Pre-clinical: The effect of octreotide on force generation was assessed during acute and prolonged drug incubation on human lymphatic vessels mounted in a myograph...
November 10, 2023: European Journal of Cardio-thoracic Surgery
https://read.qxmd.com/read/37930517/bone-morphogenetic-protein-6-induces-downregulation-of-pentraxin-3-expression-in-human-granulosa-lutein-cells-in-women-with-polycystic-ovary-syndrome
#39
JOURNAL ARTICLE
Xin Xin, Hsun-Ming Chang, Peter C K Leung, Li Dong, Jiaxi Li, Fang Lian, Haicui Wu
PURPOSE: To evaluate whether PTX3 is differentially expressed in the granulosa lutein cells derived from women with PCOS and whether BMP6 can regulate the expression of PTX3 in hGL cells. METHODS: The expression levels of BMP6 and PTX3 in granulosa lutein cells were evaluated by RT-qPCR. The correlation between the expression levels of BMP6 /PTX3 and oocyte quality indexes were analyzed using clinical samples. The cells were incubated with BMP6 at different concentrations and times to check the expression of PTX3 in KGN cells...
November 6, 2023: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/37880512/artificial-intelligence-in-time-lapse-system-advances-applications-and-future-perspectives-in-reproductive-medicine
#40
REVIEW
Thi-My-Trang Luong, Nguyen Quoc Khanh Le
With the rising demand for in vitro fertilization (IVF) cycles, there is a growing need for innovative techniques to optimize procedure outcomes. One such technique is time-lapse system (TLS) for embryo incubation, which minimizes environmental changes in the embryo culture process. TLS also significantly advances predicting embryo quality, a crucial determinant of IVF cycle success. However, the current subjective nature of embryo assessments is due to inter- and intra-observer subjectivity, resulting in highly variable results...
October 26, 2023: Journal of Assisted Reproduction and Genetics
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