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https://www.readbyqxmd.com/read/29136144/antioxidants-improve-ivf-outcome-and-subsequent-embryo-development-in-the-mouse
#1
T Truong, D K Gardner
STUDY QUESTION: What is the effect of a combination of three antioxidants (Acetyl-L-Carnitine, N-Acetyl-L-Cysteine and α-Lipoic Acid), present in IVF medium during mouse oocyte and sperm collection, on fertilization and subsequent IVF embryo development? SUMMARY ANSWER: A combination of antioxidants resulted in faster developmental times from the 2-cell stage through to expanded blastocyst stage, accompanied by a significant increase in blastocyst cell number and a reduction of intracellular hydrogen peroxide (H2O2) levels...
November 10, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29093204/in-vitro-fertilization-in-mice
#2
Robert Taft
In vitro fertilization (IVF) involves fertilization of mature oocytes with capacitated sperm in a tissue culture dish. This technique can generate large numbers of cleavage-stage embryos without using a significant number of single-caged stud males for mating. In addition, sperm penetration is synchronous during incubation with mature oocytes, leading to synchronous development, unlike fertilization in vivo, when natural ovulation is usually spread over time. These embryos can be used for a variety of applications including rapid strain expansion and generation of age-matched experimental cohorts as well as cryopreservation at the two-cell stage (speed cryo)...
November 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29062792/elevated-levels-of-serum-vascular-endothelial-growth-factor-a-are-not-related-to-nk-cell-parameters-in-recurrent-ivf-failure
#3
Rhea Bansal, Brian Ford, Shree Bhaskaran, Meenyau Thum, Amolak Bansal
BACKGROUND: Vascular Endothelial Growth Factor and NK cells have an interrelated role in angiogenesis that is critical for placentation and success of in vitro fertilization. An attempt was made to assess a possible relationship between the two in this study. METHODS: A case control study was performed comparing the serum levels of VEGF-A and its receptor VEGF-R1 with levels of NK cells, activated NK cells and NK cytotoxicity in 62 women with Repeated Implantation Failure (RIF)...
July 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28958997/effects-of-difructose-dianhydride-dfa-iv-on-in-vitro-fertilization-in-pigs
#4
Young-Joo Yi, S Kamala-Kannan, Jeong-Muk Lim, Byung-Taek Oh, Sang-Myeong Lee
Difructose dianhydride IV (DFA-IV) is produced from levan, which is a natural polysaccharide that belongs to the fructan family, through the activity of levan fructotransferase (LF) derived from microorganisms. Recently, DFA-IV has been expected to have diverse applications in the food and medical industry. Here, we examined the potential application of DFA-IV forin vitro fertilization (IVF) in pigs. In the assessment of acrosomal integrity during incubation, intact acrosomal or viable spermatozoa were highly sustained in 0...
September 26, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28938894/in-vitro-progesterone-production-by-luteinized-human-mural-granulosa-cells-is-modulated-by-activation-of-ampk-and-cause-of-infertility
#5
E C Bowdridge, M W Vernon, J A Flores, M J Clemmer
BACKGROUND: Mural granulosa cells from IVF patients were provided by the West Virginia University Center for Reproductive Medicine in Morgantown, WV. The effect of adenosine monophosphate activated protein kinase (AMPK) activation, primary cause of infertility, age, BMI, and pregnancy outcome on production of progesterone were examined separately. METHODS: Isolated mural sheets from IVF patients (n = 26) were centrifuged, supernatant discarded, and the pellet re-suspended in 500 μl of DMEM/F12...
September 22, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#6
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28865550/obstetric-and-perinatal-outcomes-of-pregnancies-conceived-with-embryos-cultured-in-a-time-lapse-monitoring-system
#7
RANDOMIZED CONTROLLED TRIAL
Maria Fernanda Insua, Ana Cristina Cobo, Zaloa Larreategui, Marcos Ferrando, Vicente Serra, Marcos Meseguer
OBJECTIVE: To compare obstetric and perinatal outcomes of singleton pregnancies resulting from embryos incubated in a time-lapse system (TLS) with those of embryos grown in standard IVF incubators (SI). DESIGN: Retrospective description of a cohort of patients who conceived during a randomized, controlled trial. SETTING: Private university-affiliated IVF center. PATIENT(S): Of 856 randomized patients, 378 gave birth to a live-born infant: 216 of the deliveries originated from embryos incubated in TLS, and 162 deliveries were from embryos cultured in SI...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28865549/effect-of-oocyte-vitrification-on-embryo-quality-time-lapse-analysis-and-morphokinetic-evaluation
#8
Ana Cobo, Aila Coello, Jose Remohí, Jose Serrano, Jose Maria de Los Santos, Marcos Meseguer
OBJECTIVE: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging. DESIGN: Observational cohort study. SETTING: University-affiliated private IVF center. PATIENT(S): Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years. INTERVENTIONS(S): None...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28712646/live-births-after-embryo-selection-using-morphokinetics-versus-conventional-morphology-a-retrospective-analysis
#9
Simon Fishel, Alison Campbell, Sue Montgomery, Rachel Smith, Lynne Nice, Samantha Duffy, Lucy Jenner, Kathyrn Berrisford, Louise Kellam, Rob Smith, Ivy D'Cruz, Ashley Beccles
The increasing corpus of clinical studies using time-lapse imaging for embryo selection demonstrates considerable variation in study protocols and only limited-sized study cohorts. Outcome measures are based on implantation or clinical pregnancy; some predict blastulation from early cleavage-stage data, and few have evaluated live birth. Erroneously, most studies treat the embryos as independent variables and do not include patient or treatment variables in the statistical analyses. In this study, cohort size was 14,793 patients and 23,762 cycles...
June 23, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28711302/potential-selection-of-genetically-balanced-spermatozoa-based-on-the-hypo-osmotic-swelling-test-in-chromosomal-rearrangement-carriers
#10
Alexandre Rouen, Léa Carlier, Solveig Heide, Matthieu Egloff, Pauline Marzin, Flavie Ader, Mathias Schwartz, Eli Rogers, Nicole Joyé, Richard Balet, Nathalie Lédée, Laura Prat-Ellenberg, Nino Guy Cassuto, Jean-Pierre Siffroi
Chromosomal translocations and other balanced rearrangements, although usually associated with a normal phenotype, can lead to the transmission of an abnormal unbalanced genome to the offspring. Balanced and unbalanced spermatozoa, being indistinguishable, cannot be selected or deselected for prior to IVF and pre-implantation genetic diagnosis. Spermatozoa from 16 chromosomal rearrangement carriers were studied. After incubation in a hypo-osmotic solution (hypo-osmotic swelling test, or HOST), spermatozoa were fixed on microscope slides...
June 27, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28632990/shortening-gametes-co-incubation-time-improves-live-birth-rate-for-couples-with-a-history-of-fragmented-embryos
#11
Anne Le Bras, Laetitia Hesters, Vanessa Gallot, Cathie Tallet, Gerard Tachdjian, Nelly Frydman
Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study was designed to evaluate the efficiency of SGCO in a poor prognosis population with a history of fragmented embryos defined by the presence of at least 50% of the embryos with more than 25% of cytoplasmic fragments...
October 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28613231/melatonin-scavenger-properties-against-oxidative-and-nitrosative-stress-impact-on-gamete-handling-and-in-vitro-embryo-production-in-humans-and-other-mammals
#12
REVIEW
Pía Loren, Raúl Sánchez, María-Elena Arias, Ricardo Felmer, Jennie Risopatrón, Carolina Cheuquemán
Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28570713/does-time-lapse-imaging-have-favorable-results-for-embryo-incubation-and-selection-compared-with-conventional-methods-in-clinical-in-vitro-fertilization-a-meta-analysis-and-systematic-review-of-randomized-controlled-trials
#13
Minghao Chen, Shiyou Wei, Junyan Hu, Jing Yuan, Fenghua Liu
OBJECTIVE: The present study aimed to undertake a review of available evidence assessing whether time-lapse imaging (TLI) has favorable outcomes for embryo incubation and selection compared with conventional methods in clinical in vitro fertilization (IVF). METHODS: Using PubMed, EMBASE, Cochrane library and ClinicalTrial.gov up to February 2017 to search for randomized controlled trials (RCTs) comparing TLI versus conventional methods. Both studies randomized women and oocytes were included...
2017: PloS One
https://www.readbyqxmd.com/read/28558842/natriuretic-peptides-improve-the-developmental-competence-of-in-vitro-cultured-porcine-oocytes
#14
Yanhao Zhang, Huarong Wang, Wei Liu, Ye Yang, Xiao Wang, Zhiyuan Zhang, Qirui Guo, Chao Wang, Guoliang Xia
BACKGROUND: Natriuretic peptides (NPs), brain and C type NPs (BNP and CNP), were involved in the maintenance of porcine oocyte meiotic arrest. The present study investigated the effects of NPs on developmental competence of immature porcine oocytes with follicles of different sizes. METHODS: Follicular fluid NP levels were examined by radioimmunoassay. The developmental competence of porcine oocytes was evaluated by cleavage and blastocyst developmental rates after in vitro fertilization (IVF) or parthenogenetic activation (PA) of cumulus oocyte complexes (COCs), which were recovered from follicle with different sizes...
May 30, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#15
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP induces sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
August 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28512855/insulin-like-growth-factor-i-enhances-the-developmental-competence-of-yak-embryos-by-modulating-aquaporin-3
#16
P Chen, Y Pan, Y Cui, Z Wen, P Liu, H He, Q Li, X Peng, T Zhao, S Yu
The objective of our present study was to determine the effects of insulin-like growth factor I (IGF-I) on the development of yak (Bos grunniens) embryos after cumulus-oocyte complex (COC) vitrification and warming followed by in vitro fertilization (IVF). In Experiment 1, the yak COCs underwent vitrification and then IVF. Embryos were incubated in synthetic oviductal fluid (SOF) supplemented with four concentrations (0, 50, 100 and 200 ng/ml) of IGF-I, while the yak COCs without vitrification or IGF-I supplementation acted as the control group; the BAX, BCL-2, AQP3mRNA and aquaporin 3 (AQP3) protein expression levels in the five groups of blastocysts were evaluated using quantitative real-time PCR and immunofluorescence analyses...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28482910/pregnancy-rates-of-day-4-and-day-5-embryos-after-culture-in-an-integrated-time-lapse-incubator
#17
Verena Holschbach, Julia Weigert, Jens Erik Dietrich, Sabine Roesner, Markus Montag, Thomas Strowitzki, Bettina Toth
BACKGROUND: The aim of this study was to compare pregnancy rates in patients undergoing IVF/ICSI with embryo transfer after 4 and 5 days of culture in a closed incubation system with integrated time-lapse imaging. METHODS: Out of n = 2207 in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) cycles performed between January 2011 and April 2016 at a tertiary referral university hospital, a total of n = 599 IVF/ICSI cycles with prolonged embryo culture in an integrated time-lapse system (EmbryoScope(©) (Vitrolife)) until day 4 or 5 were retrospectively analyzed with regard to embryo morphology and pregnancy rates...
May 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28442049/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#18
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P<0...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28319017/preliminary-investigation-of-the-prevalence-and-implantation-potential-of-abnormal-embryonic-phenotypes-assessed-using-time-lapse-imaging
#19
Amy Barrie, Roy Homburg, Garry McDowell, Jeremy Brown, Charles Kingsland, Stephen Troup
This retrospective, single site observational study aimed to delineate five abnormal embryonic developmental phenotypes, assessing their prevalence, development potential and suitability for inclusion in embryo selection models for IVF. In total, 15,819 embryos from 4559 treatment cycles cultured in EmbryoScope® incubators between January 2014 and January 2016 were included. Time-lapse images were assessed retrospectively for five abnormal embryo phenotypes: direct cleavage, reverse cleavage, absent cleavage, chaotic cleavage and cell lysis...
May 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28302936/cryotolerance-of-porcine-blastocysts-is-improved-by-treating-in-vitro-matured-oocytes-with-l-carnitine-prior-to-fertilization
#20
Jenna L Lowe, Louise K Bartolac, Roslyn Bathgate, Christopher G Grupen
Sufficient generation of adenosine triphosphate (ATP) by oocytes is critical for fertilization and embryo development. The objective of this study was to determine the effects of supplementing media with L-carnitine, a co-factor required for the metabolism of fatty acids, during the peri-fertilization period on embryo development and energy generation. Firstly, in vitro matured (IVM) porcine oocytes were co-incubated with sperm in IVF medium supplemented with 0‒24 mM L-carnitine. The blastocyst formation rate of the control group was greater than those of the L-carnitine groups (P < 0...
June 21, 2017: Journal of Reproduction and Development
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