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IVF Blastocyst Transfer

V Muller, M Makhmadalieva, I Kogan, I Fedorova, E Lesik, E Komarova, L Dzhemlikhanova, D Niauri, A Gzgzyan, E Ailamazyan
BACKGROUND: Ectopic pregnancy (EP) has been reported to occur in 1.4-5.4% of all clinical pregnancies resulting from in vitro fertilization (IVF) and embryo transfer (ET). Data on factors associated with abnormal embryo implantation following assisted conception are limited. MATERIALS AND METHODS: A systematic review and meta-analysis was performed to determine whether there is an association between the day (cleavage-stage, D3, versus blastocyst, D5) or the type (fresh versus frozen/thawed) of ET and EP rate...
October 2016: Gynecological Endocrinology
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
October 3, 2016: Reproductive Biomedicine Online
Amanda Souza Setti, Rita Cássia Sávio Figueira, Daniela Paes de Almeida Ferreira Braga, Matheus de Castro Azevedo, Assumpto Iaconelli, Edson Borges
OBJECTIVE: To study whether embryos derived from oocytes presenting a smooth endoplasmic reticulum cluster (SERC) are less likely to develop into blastocysts and implant. DESIGN: Transversal study. SETTING: Private university-affiliated in vitro fertilization (IVF) center. PATIENT(S): Total of 7,609 oocytes obtained from 743 intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Oocytes split between the SERC-positive cycles (with at least one SERC-positive oocyte) and the SERC-negative cycles (only oocytes free of SERC)...
October 12, 2016: Fertility and Sterility
W P Martins, C O Nastri, L Rienzi, S Z van der Poel, C Gracia, C Racowsky
OBJECTIVES: Blastocyst transfer could be advantageous because it allows a more physiologic timing of exposure of the embryo to the uterine environment and permit embryo self-selection after activation of the embryonic genome on day 3. Conversely, the in vitro environment is probably inferior to that provided in vivo and in vitro culture beyond embryonic genomic activation could potentially harm embryos. Our objective was to identify, appraise and summarize the available evidence comparing the effectiveness of blastocyst and cleavage stage embryo transfer...
October 12, 2016: Ultrasound in Obstetrics & Gynecology
Jin Hee Eum, Jae Kyun Park, So Young Kim, Soo Kyung Paek, Hyun Ha Seok, Eun Mi Chang, Dong Ryul Lee, Woo Sik Lee
OBJECTIVE: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients...
September 2016: Clinical and Experimental Reproductive Medicine
Stefan C Kane, Elissa Willats, Sammya Bezerra Maia E Holanda Moura, Jonathan Hyett, Fabrício da Silva Costa
Chromosomal aneuploidy is responsible for a significant proportion of pregnancy failures, whether conceived naturally or through in vitro fertilization (IVF). In an effort to improve the success rate of IVF, screening embryos for aneuploidy - or pre-implantation genetic screening (PGS) - has been proposed as a means of ensuring only euploid embryos are selected for transfer. Early PGS approaches were based on fluorescence in situ hybridization testing, and have been shown not to improve live birth rates. Recent developments in genetic testing technologies - such as next-generation sequencing and quantitative polymerase chain reaction, coupled with embryo biopsy at the blastocyst stage - have shown promise in improving IVF outcomes, but they remain to be validated in adequately powered, prospective randomized trials...
September 29, 2016: Fetal Diagnosis and Therapy
Juan-Enrique Schwarze, Javier A Crosby, Fernando Zegers-Hochschild
OBJECTIVE: To determine whether the addition of either urinary or recombinant LH in patients undergoing routine clinical care improved the outcome in terms of the number of oocytes recovered for insemination or the delivery rate per initiated cycle. DESIGN: Cohort analysis. PATIENT(S): Couples undergoing IVF/ICSI in 158 institutions in 15 countries in Latin America. SETTING: In vitro fertilization clinics. INTERVENTION(S): We compared the outcome of three different protocols of COH, including rFSH only, rFSH plus rLH, and rFSH plus hMG...
September 24, 2016: Fertility and Sterility
Georgina M Chambers, Handan Wand, Alan Macaldowie, Michael G Chapman, Cynthia M Farquhar, Mark Bowman, David Molloy, William Ledger
STUDY QUESTION: Have ART live birth rates improved in Australia over the last 12 years? SUMMARY ANSWER: There were striking improvements in per-cycle live birth rates observed for frozen/thaw embryo transfers, blastocyst transfer and single embryo transfer (SET), while live birth rates following ICSI were lower than IVF for non-male factor infertility in most years. WHAT IS ALREADY KNOWN: ART and associated techniques have become the predominant treatment of infertility over the past 30 years in most developed countries...
September 22, 2016: Human Reproduction
Georgios Christopoulos, Antonios Vlismas, Anna Carby, Stuart Lavery, Geoffrey Trew
A retrospective, cohort study of high-risk patients undergoing IVF treatment was performed to assess if there is a difference in clinical pregnancy rate, live birth rate and the incidence of ovarian hyperstimulation syndrome, when a GnRH agonist (GnRHa) trigger with intensive luteal support is compared to human chorionic gonadotropin (hCG) with standard luteal support. The control group consisted of 382 high-risk patients having a GnRH antagonist protocol with 194 receiving an hCG trigger. All patients had ≥18 follicles ≥11mm or serum oestradiol >18,000pmol/l on the day of trigger...
September 2016: Human Fertility: Journal of the British Fertility Society
Laura Lindsay, Samson N Dowland, Christopher R Murphy
Controlled ovarian hyperstimulation is an essential component of IVF techniques to ensure proliferation and development of multiple ovarian follicules but the effects of these hormones on the endometrium are largely unknown. During normal pregnancy in rats, there are significant changes in the basal plasma membrane of uterine epithelial cells (UECs) at the time of receptivity, including loss of focal adhesions. This enables the UECs to be removed from the implantation chamber surrounding the blastocyst, thus allowing invasion into the underlying stroma...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
D Castelló, Y Motato, N Basile, J Remohí, M Espejo-Catena, M Meseguer
Can the time-lapse system (TLS) identify the best embryo for transfer? Although there are several studies that support this hypothesis, more research is required to improve the quality of the current evidence and also to assess live birth rate, miscarriage, stillbirth or clinical pregnancy in order to choose between a TLS or conventional incubation. In addition, although some authors report on effectiveness and safety in the use of TLS monitoring of embryo development in vitro, other authors that have not found relevant differences between the two systems for the culture and subsequence embryo selection...
October 2016: Molecular Human Reproduction
George Α Sigalos, Olga Triantafyllidou, Nikos F Vlahos
PURPOSE: The final success of an IVF attempt depends on several steps and decisions taken during the ovarian stimulation, the oocyte retrieval, the embryo culture and the embryo transfer. The final selection of the embryos most likely to implant is the final step in this process and the responsibility of the lab. Apart from strict morphologic criteria that historically have been used in embryo selection, additional information on genetic, metabolomic and morphokinetic characteristics of the embryo is recently combined to morphology to select the embryo most likely to produce a pregnancy...
September 15, 2016: Archives of Gynecology and Obstetrics
Dorit C Kieslinger, Stefanie De Gheselle, Cornelis B Lambalk, Petra De Sutter, E Hanna Kostelijk, Jos W R Twisk, Joukje van Rijswijk, Etienne Van den Abbeel, Carlijn G Vergouw
STUDY QUESTION: Does prospective embryo selection using the results from the Eava Test (Early Embryo Viability Assessment) in combination with standard morphology increase the pregnancy rate of IVF and ICSI patients compared to embryo selection based on morphology only? SUMMARY ANSWER: Embryo selection using the Eeva Test plus standard morphology on Day 3 results in comparable pregnancy rates as conventional morphological embryo selection. WHAT IS KNOWN ALREADY: Time-lapse monitoring of embryo development may represent a superior way to culture and select embryos in vitro...
September 12, 2016: Human Reproduction
Jorge Rodriguez-Purata, Julian Gingold, Joseph Lee, Michael Whitehouse, Richard Slifkin, Christine Briton-Jones, Alan Copperman, Benjamin Sandler
STUDY QUESTION: Do the reproductive outcomes from the transfer of fully hatched (FH) blastocysts differ from those of not fully hatched (NFH) blastocysts? SUMMARY ANSWER: Biochemical pregnancy rate (BPR), implantation rate (IR), live birth rate (LBR) and early pregnancy loss (EPL) rate are similar in FH and NFH single euploid blastocyst embryo transfers. WHAT IS KNOWN ALREADY: The use of extended culture and PGS often leads to transfer of an embryo that is well developed and frequently FH from the zona pellucida...
September 12, 2016: Human Reproduction
Anick De Vos, Lisbet Van Landuyt, Samuel Santos-Ribeiro, Michel Camus, Hilde Van de Velde, Herman Tournaye, Greta Verheyen
STUDY QUESTION: Do cumulative live birth rates differ between single cleavage-stage Day 3 transfer and single blastocyst-stage Day 5 transfer? SUMMARY ANSWER: Cumulative live birth rates after Day 3 and 5 transfers were similar in young patients when the vitrified embryo transfers were also taken into account. WHAT IS KNOWN ALREADY: Previous evidence has shown that the probability of live birth following IVF with a fresh embryo transfer is significantly higher after blastocyst-stage Day 5 transfer...
September 12, 2016: Human Reproduction
R H Goldman, D J Kaser, S A Missmer, S S Srouji, L V Farland, C Racowsky
PURPOSE: Many practices are moving away from cleavage-stage transfer in favor of blastocyst transfer. The purpose of this study is to evaluate how the overall live birth rate for fresh IVF cycles may increase by optimizing the day of transfer for each patient. METHODS: This is a retrospective cohort study of 1225 first fresh autologous IVF cycles performed between May 2012 and November 2013. Stepwise logistic regression was used to determine characteristics associated with live birth following cleavage-stage versus blastocyst transfer...
September 10, 2016: Journal of Assisted Reproduction and Genetics
Hiroki Kurosawa, Hiroki Utsunomiya, Naomi Shiga, Aiko Takahashi, Motomasa Ihara, Masumi Ishibashi, Mitsuo Nishimoto, Zen Watanabe, Hiroyuki Abe, Jin Kumagai, Yukihiro Terada, Hideki Igarashi, Toshifumi Takahashi, Atsushi Fukui, Ryota Suganuma, Masahito Tachibana, Nobuo Yaegashi
STUDY QUESTION: Does a new system-the chip-sensing embryo respiration monitoring system (CERMs)-enable evaluation of embryo viability for potential application in a clinical IVF setting? SUMMARY ANSWER: The system enabled the oxygen consumption rate of spheroids, bovine embryos and frozen-thawed human embryos to be measured, and this rate corresponded to the developmental potential of embryos. WHAT IS ALREADY KNOWN: To date, no reliable and clinically suitable objective evaluation methods for embryos are available, which circumvent the differences in inter-observer subjective view...
October 2016: Human Reproduction
Bjørn Molt Petersen, Mikkel Boel, Markus Montag, David K Gardner
STUDY QUESTION: Can a generally applicable morphokinetic algorithm suitable for Day 3 transfers of time-lapse monitored embryos originating from different culture conditions and fertilization methods be developed for the purpose of supporting the embryologist's decision on which embryo to transfer back to the patient in assisted reproduction? SUMMARY ANSWER: The algorithm presented here can be used independently of culture conditions and fertilization method and provides predictive power not surpassed by other published algorithms for ranking embryos according to their blastocyst formation potential...
October 2016: Human Reproduction
Norbert Gleicher, Andrea Vidali, Jeffrey Braverman, Vitaly A Kushnir, David H Barad, Cynthia Hudson, Yang-Guan Wu, Qi Wang, Lin Zhang, David F Albertini
BACKGROUND: To preclude transfer of aneuploid embryos, current preimplantation genetic screening (PGS) usually involves one trophectoderm biopsy at blastocyst stage, assumed to represent embryo ploidy. Whether one such biopsy can correctly assess embryo ploidy has recently, however, been questioned. METHODS: This descriptive study investigated accuracy of PGS in two ways. Part I: Two infertile couples donated 11 embryos, previously diagnosed as aneuploid and, therefore, destined to be discarded...
September 5, 2016: Reproductive Biology and Endocrinology: RB&E
Hideki Igarashi, Toshifumi Takahashi, Hiroyuki Abe, Hiroshi Nakano, Osamu Nakajima, Satoru Nagase
STUDY QUESTION: Does in vivo aging of mouse oocytes affect mitochondrial function? SUMMARY ANSWER: Mitochondrial function was impaired in post-ovulatory in vivo-aged mouse oocytes and microinjection of somatic cell mitochondria did not rescue poor fertilization and embryonic development rates. WHAT IS KNOWN ALREADY: The mechanisms underlying the decline in oocyte quality associated with oocyte aging remain unknown, although studies have suggested that the decline is regulated by mitochondrial dysfunction...
October 2016: Human Reproduction
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