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IVF Blastocyst Transfer

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https://www.readbyqxmd.com/read/28070808/combined-positive-effect-of-oocyte-extracts-and-brilliant-cresyl-blue-stained-recipient-cytoplasts-on-epigenetic-reprogramming-and-gene-expression-in-buffalo-nuclear-transfer-embryos
#1
E M Sadeesh, Shah Fozia, Kataria Meena
This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P < 0.05) and HDAC-1, DNMT-1, and DNMT-3A decreased (P < 0.05) in extract treated cells (ETCs)...
January 9, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#2
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28050963/live-births-after-polar-body-biopsy-and-frozen-thawed-cleavage-stage-embryo-transfer-case-report
#3
Fernando Guimarães, Matheus Roque, Marcello Valle, Alessandra Kostolias, Rodrigo A de Azevedo, Ciro D Martinhago, Marcos Sampaio, Selmo Geber
Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050953/mir-142-3p-as-a-biomarker-of-blastocyst-implantation-failure-a-pilot-study
#4
Edson Borges, Amanda Souza Setti, Daniela P A F Braga, Murilo V Geraldo, Rita de Cássia S Figueira, Assumpto Iaconelli
OBJECTIVE: This study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation. METHODS: Culture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28031323/inter-observer-and-intra-observer-agreement-between-embryologists-during-selection-of-a-single-day-5-embryo-for-transfer-a-multicenter-study
#5
Ashleigh Storr, Christos A Venetis, Simon Cooke, Suha Kilani, William Ledger
STUDY QUESTION: What is the inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer? SUMMARY ANSWER: The inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer was generally good, although not optimal, even among experienced embryologists. WHAT IS KNOWN ALREADY: Previous research on the morphological assessment of early stage (two pronuclei to Day 3) embryos has shown varying levels of inter-observer and intra-observer agreement...
December 28, 2016: Human Reproduction
https://www.readbyqxmd.com/read/28017685/physical-exercise-before-pregnancy-helps-the-development-of-mouse-embryos-produced-in-vitro
#6
Wen Hai Xu, Hao Wu, Wei Lan Xia, Hui Lan, Yongsheng Wang, Yong Zhang, Song Hua
Effects of pre-gestational physical activity on the later development of embryos generated in vitro were evaluated. Kunming mice were divided into two groups, namely exercised and unexercised, with the former undergoing physical training on a motor-driven leveled treadmill over a period of 4weeks (5days/week and 60min/day). After that, following superovulation, collection of oocytes from both groups was performed for in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Notably and specifically, natural mating between the unexercised mice was also done for in vivo fertilization (IVIF)...
December 23, 2016: Mitochondrion
https://www.readbyqxmd.com/read/28003868/outcome-of-one-hundred-consecutive-icsi-attempts-using-patient-operated-home-sonography-for-monitoring-follicular-growth
#7
J Gerris, F Vandekerckhove, P De Sutter
OBJECTIVE: cohort study describing clinical and laboratory outcomes in ICSI patients using self-operated endovaginal tele-monitoring (SOET). SETTING: University department of reproductive medicine. PATIENTS: 78 patients undergoing 100 consecutive ICSI attempts. INTERVENTIONS: patients' recorded vaginal sonograms and sent recordings using the cloud based Sonaura device to the care provider who procured responses, avoiding hospital visits...
September 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27986817/the-slow-growing-embryo-and-premature-progesterone-elevation-compounding-factors-for-embryo-endometrial-asynchrony
#8
Mae Wu Healy, Meghan Yamasaki, George Patounakis, Kevin S Richter, Kate Devine, Alan H DeCherney, Micah J Hill
STUDY QUESTION: Is there an association of progesterone (P4) on the day of trigger with live birth in autologous ART transfer cycles on day 5 versus day 6? SUMMARY ANSWER: P4 had a greater negative effect on live birth in day 6 fresh transfers compared to day 5 fresh transfers. WHAT IS KNOWN ALREADY: Premature P4 elevation is associated with lower live birth rates in fresh autologous ART cycles, likely due to worsened endometrial-embryo asynchrony...
December 16, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27918161/-current-options-of-preimplantion-genetic-screening-and-preimplantation-genetic-diagnostics
#9
V Šimečková
OBJECTIVE: The aim of this work is to summarize the current knowledge about preimplantation genetic screening and diagnostics. DESIGN: A review article. SETTING: Department of Gynecology and Obstetrics, District Hospital Šternberk, IVF Clinic, Olomouc. RESULTS: Preimplantation genetic testing is a complex of genetic and molecular cytogenetic examinations, which can help to detect abnormalities in embryos before transfer into the uterus of the mother...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27894197/temporal-validation-of-a-simplified-blastocyst-grading-system
#10
Alison Richardson, Sophie Brearley, Saran Ahitan, Sarah Chamberlain, Tracey Davey, Lyndsey Zujovic, James Hopkisson, Bruce Campbell, Nick Raine-Fenning
We have recently established the clinical effectiveness and credibility of a simplified blastocyst grading system by demonstrating its prognostic potential and the inter- and intra-observer variability associated with it. To be considered clinically useful, however, the grading system also needs to be accurate (i.e. well calibrated with good discriminative ability). This study prospectively evaluates the performance of the grading system on subsequent patients from the same IVF unit in an attempt to temporally validate the model...
November 28, 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27874292/granulocyte-colony-stimulating-factor-treatment-of-resistant-thin-endometrium-in-women-with-frozen-thawed-blastocyst-transfer
#11
Michał Kunicki, Krzysztof Łukaszuk, Joanna Liss, Patrycja Skowrońska, Joanna Szczyptańska
: The aim of the study was to assess the granulocyte-colony stimulating factor (G-CSF) effect on unresponsive thin (<7 mm) endometrium in women undergoing frozen-thawed embryo transfer at the blastocyst stage. A total of 62 women with thin unresponsive endometrium were included in the study, of which, 29 received a G-CSF infusion and 33 who opted out of the study served as controls. Patients in both groups had similar endometrial thickness at the time of the initial evaluation: 6.50 mm (5...
November 22, 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27852688/the-role-of-intracytoplasmic-sperm-injection-in-non-male-factor-infertility-in-advanced-maternal-age
#12
Samer Tannus, Weon-Young Son, Ashley Gilman, Grace Younes, Tal Shavit, Michael-Haim Dahan
STUDY QUESTION: Does ICSI improve reproductive outcomes compared with conventional IVF when used for non-male factor infertility in women aged 40 years and over? SUMMARY ANSWER: There is no advantage of ICSI over conventional IVF in women aged 40 years and over when used for non-male factor infertility. WHAT IS KNOWN ALREADY: The use of ICSI has increased dramatically in recent years and is being applied for indications other than male factor infertility...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27852632/predicting-the-chances-of-a-live-birth-after-one-or-more-complete-cycles-of-in-vitro-fertilisation-population-based-study-of-linked-cycle-data-from-113%C3%A2-873-women
#13
David J McLernon, Ewout W Steyerberg, Egbert R Te Velde, Amanda J Lee, Siladitya Bhattacharya
OBJECTIVE:  To develop a prediction model to estimate the chances of a live birth over multiple complete cycles of in vitro fertilisation (IVF) based on a couple's specific characteristics and treatment information. DESIGN:  Population based cohort study. SETTING:  All licensed IVF clinics in the UK. National data from the Human Fertilisation and Embryology Authority register. PARTICIPANTS:  All 253 417 women who started IVF (including intracytoplasmic sperm injection) treatment in the UK from 1999 to 2008 using their own eggs and partner's sperm...
November 16, 2016: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/27827818/oocyte-embryo-and-blastocyst-cryopreservation-in-art-systematic-review-and-meta-analysis-comparing-slow-freezing-versus-vitrification-to-produce-evidence-for-the-development-of-global-guidance
#14
REVIEW
Laura Rienzi, Clarisa Gracia, Roberta Maggiulli, Andrew R LaBarbera, Daniel J Kaser, Filippo M Ubaldi, Sheryl Vanderpoel, Catherine Racowsky
BACKGROUND: Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular dehydration while minimizing intracellular ice formation. Vitrification allows the solidification of the cell(s) and of the extracellular milieu into a glass-like state without the formation of ice...
November 8, 2016: Human Reproduction Update
https://www.readbyqxmd.com/read/27759446/ectopic-pregnancy-following-in-vitro-fertilization-meta-analysis-and-single-center-experience-during-6-years
#15
V Muller, M Makhmadalieva, I Kogan, I Fedorova, E Lesik, E Komarova, L Dzhemlikhanova, D Niauri, A Gzgzyan, E Ailamazyan
BACKGROUND: Ectopic pregnancy (EP) has been reported to occur in 1.4-5.4% of all clinical pregnancies resulting from in vitro fertilization (IVF) and embryo transfer (ET). Data on factors associated with abnormal embryo implantation following assisted conception are limited. MATERIALS AND METHODS: A systematic review and meta-analysis was performed to determine whether there is an association between the day (cleavage-stage, D3, versus blastocyst, D5) or the type (fresh versus frozen/thawed) of ET and EP rate...
October 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27746110/a-direct-healthcare-cost-analysis-of-the-cryopreserved-versus-fresh-transfer-policy-at-the-blastocyst-stage
#16
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
January 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27743693/oocytes-with-smooth-endoplasmic-reticulum-clusters-originate-blastocysts-with-impaired-implantation-potential
#17
Amanda Souza Setti, Rita Cássia Sávio Figueira, Daniela Paes de Almeida Ferreira Braga, Matheus de Castro Azevedo, Assumpto Iaconelli, Edson Borges
OBJECTIVE: To study whether embryos derived from oocytes presenting a smooth endoplasmic reticulum cluster (SERC) are less likely to develop into blastocysts and implant. DESIGN: Transversal study. SETTING: Private university-affiliated in vitro fertilization (IVF) center. PATIENT(S): Total of 7,609 oocytes obtained from 743 intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Oocytes split between the SERC-positive cycles (with at least one SERC-positive oocyte) and the SERC-negative cycles (only oocytes free of SERC)...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27731533/blastocyst-versus-cleavage-stage-embryo-transfer-a-systematic-review-and-meta-analysis-of-the-reproductive-outcomes
#18
W P Martins, C O Nastri, L Rienzi, S Z van der Poel, C Gracia, C Racowsky
OBJECTIVES: Blastocyst transfer could be advantageous because it allows a more physiologic timing of exposure of the embryo to the uterine environment and permit embryo self-selection after activation of the embryonic genome on day 3. Conversely, the in vitro environment is probably inferior to that provided in vivo and in vitro culture beyond embryonic genomic activation could potentially harm embryos. Our objective was to identify, appraise and summarize the available evidence comparing the effectiveness of blastocyst and cleavage stage embryo transfer...
October 12, 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27689039/clinical-outcomes-of-single-versus-double-blastocyst-transfer-in-fresh-and-vitrified-warmed-cycles
#19
Jin Hee Eum, Jae Kyun Park, So Young Kim, Soo Kyung Paek, Hyun Ha Seok, Eun Mi Chang, Dong Ryul Lee, Woo Sik Lee
OBJECTIVE: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27682145/pre-implantation-genetic-screening-techniques-implications-for-clinical-prenatal-diagnosis
#20
Stefan C Kane, Elissa Willats, Sammya Bezerra Maia E Holanda Moura, Jonathan Hyett, Fabrício da Silva Costa
Chromosomal aneuploidy is responsible for a significant proportion of pregnancy failures, whether conceived naturally or through in vitro fertilization (IVF). In an effort to improve the success rate of IVF, screening embryos for aneuploidy - or pre-implantation genetic screening (PGS) - has been proposed as a means of ensuring only euploid embryos are selected for transfer. Early PGS approaches were based on fluorescence in situ hybridization testing, and have been shown not to improve live birth rates. Recent developments in genetic testing technologies - such as next-generation sequencing and quantitative polymerase chain reaction, coupled with embryo biopsy at the blastocyst stage - have shown promise in improving IVF outcomes, but they remain to be validated in adequately powered, prospective randomized trials...
2016: Fetal Diagnosis and Therapy
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