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IVF Blastocyst Transfer

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https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#1
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#2
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28500451/pregnancy-rates-for-single-embryo-transfer-set-of-day-5-and-day-6-blastocysts-after-cryopreservation-by-vitrification-and-slow-freeze
#3
Leah Kaye, Erica Anspach Will, Alison Bartolucci, John Nulsen, Claudio Benadiva, Lawrence Engmann
PURPOSE: The purpose of this study was to compare clinical and ongoing pregnancy rates in cycles with single embryo transfer (SET) of blastocysts cryopreserved on day 5 or day 6. Our aim was to determine whether day 6 blastocysts perform adequately to recommend SET. METHODS: Retrospective cohort study including 468 transfer cycles for 392 women younger than age 38 undergoing SET at a university-affiliated IVF clinic in the USA. A total of 261 day 5 blastocysts and 207 day 6 blastocysts for frozen-thawed SET between 2010 and 2016 were analyzed...
May 12, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28472983/comparative-neonatal-outcomes-in-singleton-births-from-blastocyst-transfers-or-cleavage-stage-embryo-transfers-a-systematic-review-and-meta-analysis
#4
REVIEW
Xingling Wang, Mingze Du, Yichun Guan, Bijun Wang, Junwei Zhang, Zihua Liu
BACKGROUND: Comparative neonatal outcomes with respect to singleton births from blastocyst transfers or cleavage-state embryo transfers are controversial with respect to which method is superior. Many studies have yielded contradictory results. We performed a systematic review and meta-analysis for the purpose of comparing neonatal outcomes in single births following IVF/ICSI. METHODS: We searched the Medline, Embase and Cochrane Central Register of Clinical Trials (CCTR) databases until October 2016...
May 4, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28455751/national-trends-and-outcomes-of-autologous-in-vitro-fertilization-cycles-among-women-ages-40-years-and-older
#5
Heather Hipp, Sara Crawford, Jennifer F Kawwass, Sheree L Boulet, David A Grainger, Dmitry M Kissin, Denise Jamieson
PURPOSE: The purpose of the study was to describe trends in and investigate variables associated with clinical pregnancy and live birth in autologous in vitro fertilization (IVF) cycles among women ≥40 years. METHODS: We used autologous IVF cycle data from the National ART Surveillance System (NASS) for women ≥40 years at cycle start. We assessed trends in fresh and frozen cycles (n = 371,536) from 1996 to 2013. We reported perinatal outcomes and determined variables associated with clinical pregnancy and live birth in fresh cycles between 2007 and 2013...
April 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28443134/characterization-of-x-chromosome-gene-expression-in-bovine-blastocysts-derived-by-in-vitro-fertilization-and-somatic-cell-nuclear-transfer
#6
Byungkuk Min, Jung Sun Park, Kyuheum Jeon, Yong-Kook Kang
To better understand X-chromosome reactivation (XCR) during early development, we analyzed transcriptomic data obtained from bovine male and female blastocysts derived by in-vitro fertilization (IVF) or somatic-cell nuclear transfer (SCNT). We found that X-linked genes were upregulated by almost two-fold in female compared with male IVF blastocysts. The upregulation of X-linked genes in female IVFs indicated a transcriptional dimorphism between the sexes, because the mean autosomal gene expression levels were relatively constant, regardless of sex...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28442046/morphometric-developmental-pattern-of-bovine-handmade-cloned-concepti-in-late-pregnancy
#7
R P C Gerger, F C Zago, E S Ribeiro, S Gaudencio Neto, L T Martins, L H Aguiar, V H V Rodrigues, F H Furlan, I Ortigari, R D Sainz, C L Ferrell, M A Miglino, C E Ambrósio, J L Rodrigues, R Rossetto, F Forell, L R Bertolini, M Bertolini
Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy diagnosis was performed on Day 30 following blastocyst transfer on Day 7. Conceptus morphometry was assessed by ultrasonography on Day 51, and on Day 225 pregnant cows were killed for morphological examination of concepti...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28433373/preimplantation-genetic-diagnosis-for-aneuploidy-testing-in-women-older-than-44-years-a-multicenter-experience
#8
Filippo Maria Ubaldi, Danilo Cimadomo, Antonio Capalbo, Alberto Vaiarelli, Laura Buffo, Elisabetta Trabucco, Susanna Ferrero, Elena Albani, Laura Rienzi, Paolo E Levi Setti
OBJECTIVE: To report laboratory and clinical outcomes in preimplantation genetic diagnosis for aneuploidies (PGD-A) cycles for women 44 to 47 years old. DESIGN: Multicenter, longitudinal, observational study. SETTING: In vitro fertilization (IVF) centers. PATIENT(S): One hundred and thirty-seven women aged 44.7 ± 0.7 years (range: 44.0-46.7) undergoing 150 PGD-A cycles during April 2013 to January 2016. INTERVENTION(S): Quantitative polymerase chain reaction-based PGD-A on trophectoderm biopsies and cryopreserved euploid single-embryo transfer (SET)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433367/abnormal-implantation-after-fresh-and-frozen-in%C3%A2-vitro-fertilization-cycles
#9
Erica T Wang, Anupama S Q Kathiresan, Catherine Bresee, Naomi Greene, Carolyn Alexander, Margareta D Pisarska
OBJECTIVE: To determine whether fresh embryo transfers are at a higher risk of abnormal implantation compared with frozen embryo transfers while accounting for the embryo stage at transfer. DESIGN: Retrospective cohort study. SETTING: Not applicable. PATIENT(S): We used data from the Society for Assisted Reproductive Technologies to identify all fresh and frozen autologous IVF cycles from 2004-2013 resulting in a positive pregnancy test...
May 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28431828/clinical-use-of-monopronucleated-zygotes-following-blastocyst-culture-and-preimplantation-genetic-screening-including-verification-of-biparental-chromosome-inheritance
#10
Cara K Bradley, Maria Traversa, Natalie Hobson, Alison J Gee, Steven J McArthur
In assisted reproduction, embryos derived from monopronucleated (1PN) zygotes are considered abnormal and unsuitable for clinical use. Outcomes of 1PN-derived embryos designated for preimplantation genetic screening (PGS) were analysed. These embryos, especially from intracytoplasmic sperm injection (ICSI), were found to have a low developmental potential; 1PN and 2PN day 5 blastocyst development for IVF was 14.8% versus 36.4% (P < 0.0001), and for ICSI, 6.6% versus 34.0% (P < 0.0001), respectively. With the use of comparative genomic hybridization or next-generation sequencing, PGS was successfully carried out for 74 IVF and 32 ICSI 1PN-derived blastocysts, revealing adjusted abnormality rates of 39...
March 27, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28428943/pathogenic-variant-in-nlrp7-19q13-42-associated-with-recurrent-gestational-trophoblastic-disease-data-from-early-embryo-development-observed-during-in-vitro-fertilization
#11
E Scott Sills, Alexandra J Obregon-Tito, Harry Gao, Thomas K McWilliams, Anthony T Gordon, Catharine A Adams, Rima Slim
OBJECTIVE: To describe in vitro development of human embryos derived from an individual with a homozygous pathogenic variant in NLRP7 (19q13.42) and recurrent hydatidiform mole (HM), an autosomal recessive condition thought to occur secondary to an oocyte defect. METHODS: A patient with five consecutive HM pregnancies was genomically evaluated via next generation sequencing followed by controlled ovarian hyperstimulation, in vitro fertilization (IVF) with intracytoplasmic sperm injection, embryo culture, and preimplantation genetic screening...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28421495/chapter-11-human-embryo-vitrification
#12
Juergen Liebermann
Cryopreservation is one of the keystones in clinical infertility treatment. In particular vitrification has become a well-established and widely used routine procedure that has allowed important expansion of therapeutic strategies when IVF is used to treat infertility. Vitrification of human blastocysts allows us to maximize the potential for conception from any single in vitro fertilization cycle and prevents wastage of embryos. The technology may even be used to eliminate fresh embryo transfers for reasons of convenience, uterine receptivity, fertility preservation, preimplantation genetic diagnosis, or emergency management...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28406938/dual-effect-of-fetal-bovine-serum-on-early-development-depends-on-stage-specific-reactive-oxygen-species-demands-in-pigs
#13
Seong-Eun Mun, Bo-Woong Sim, Seung-Bin Yoon, Pil-Soo Jeong, Hae-Jun Yang, Seon-A Choi, Young-Ho Park, Young-Hyun Kim, Philyong Kang, Kang-Jin Jeong, Youngjeon Lee, Yeung Bae Jin, Bong-Seok Song, Ji-Su Kim, Jae-Won Huh, Sang-Rae Lee, Young-Kuk Choo, Sun-Uk Kim, Kyu-Tae Chang
Despite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embryos highly depends on the combination of FBS supplementation timing and embryonic reactive oxygen species (ROS) requirements. Interestingly, FBS significantly reduced intracellular ROS levels in parthenogenetically activated (PA) embryos regardless of the developmental stage...
2017: PloS One
https://www.readbyqxmd.com/read/28402555/deregulation-of-the-endometrial-stromal-cell-secretome-precedes-embryo-implantation-failure
#14
Ruban Rex Peter Durairaj, Asma Aberkane, Lukasz Polanski, Yojiro Maruyama, Miriam Baumgarten, Emma S Lucas, Siobhan Quenby, Jerry K Y Chan, Nick Raine-Fenning, Jan J Brosens, Hilde Van de Velde, Yie Hou Lee
STUDY QUESTION: Is implantation failure following ART associated with a perturbed decidual response in endometrial stromal cells (EnSCs)? SUMMARY ANSWER: Dynamic changes in the secretome of decidualizing EnSCs underpin the transition of a hostile to a supportive endometrial microenvironment for embryo implantation; perturbation in this transitional pathway prior to ART is associated with implantation failure. WHAT IS KNOWN ALREADY: Implantation is the rate-limiting step in ART, although the contribution of an aberrant endometrial microenvironment in IVF failure remains ill defined...
April 10, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28393271/analysis-of-implantation-and-ongoing-pregnancy-rates-following-the-transfer-of-mosaic-diploid-aneuploid-blastocysts
#15
Elpida Fragouli, Samer Alfarawati, Katharina Spath, Dhruti Babariya, Nicoletta Tarozzi, Andrea Borini, Dagan Wells
Preimplantation genetic testing for aneuploidy (PGT-A) is widely used in IVF and aims to improve outcomes by avoiding aneuploid embryo transfers. Chromosomal mosaicism is extremely common in early development and could affect the efficacy of PGT-A by causing incorrect embryo classification. Recent innovations have allowed accurate mosaicism detection in trophectoderm samples taken from blastocysts. However, there is little data concerning the impact of mosaicism on viability, and the optimal clinical pathway for such embryos is unclear...
April 9, 2017: Human Genetics
https://www.readbyqxmd.com/read/28390692/detection-of-mosaicism-at-blastocyst-stage-with-the-use-of-high-resolution-next-generation-sequencing
#16
REVIEW
Santiago Munné, Dagan Wells
A significant proportion of human preimplantation embryos produced during the course of in vitro fertilization (IVF) treatments contain two or more cytogenetically distinct cell lines. This phenomenon, known as chromosomal mosaicism, can involve the presence of cells with different types of aneuploidy in the absence of any normal cells or a mixture of euploid and abnormal cells. Although a high prevalence of mosaicism at the cleavage and blastocyst stages has been appreciated for two decades, the precise frequency of the phenomenon and its consequences for embryo viability have been difficult to quantify...
April 6, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28387858/mitochondrial-dna-quantification-as-a-tool-for-embryo-viability-assessment-retrospective-analysis-of-data-from-single-euploid-blastocyst-transfers
#17
K Ravichandran, C McCaffrey, J Grifo, A Morales, M Perloe, S Munne, D Wells, E Fragouli
STUDY QUESTION: Does the amount of mitochondrial DNA (mtDNA) in blastocyst biopsy specimens have the potential to serve as a biomarker of euploid embryo implantation ability, independent of morphology? SUMMARY ANSWER: The results of this study strongly suggest that elevated mtDNA levels, above a previously defined threshold, are strongly associated with blastocyst implantation failure and represent an independent biomarker of embryo viability. WHAT IS KNOWN ALREADY: Improved methods of embryo selection are highly desirable in order to increase the efficiency of IVF treatment...
June 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28386815/endometrial-injury-the-quality-of-embryos-and-blastocyst-transfer-are-the-most-important-prognostic-factors-for-in-vitro-fertilization-success-after-previous-repeated-unsuccessful-attempts
#18
Milan Reljič, Jure Knez, Vilma Kovač, Borut Kovačič
PURPOSE: The purpose of this study was to find out the most important prognostic factors for achieving a pregnancy after in vitro fertilization (IVF) in women with history of repeated unsuccessful IVF attempts. METHODS: We analyzed factors affecting pregnancy rate in a retrospective study including 429 IVF/ICSI cycles performed in women younger than 40 years with at least three previous consecutive failed IVF/ICSI attempts. RESULTS: Clinical pregnancy was observed in 140/429 (32...
April 6, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28385334/live-birth-derived-from-oocyte-spindle-transfer-to-prevent-mitochondrial-disease
#19
John Zhang, Hui Liu, Shiyu Luo, Zhuo Lu, Alejandro Chávez-Badiola, Zitao Liu, Mingxue Yang, Zaher Merhi, Sherman J Silber, Santiago Munné, Michalis Konstandinidis, Dagan Wells, Taosheng Huang
Mutations in mitochondrial DNA (mtDNA) are maternally inherited and can cause fatal or debilitating mitochondrial disorders. The severity of clinical symptoms is often associated with the level of mtDNA mutation load or degree of heteroplasmy. Current clinical options to prevent transmission of mtDNA mutations to offspring are limited. Experimental spindle transfer in metaphase II oocytes, also called mitochondrial replacement therapy, is a novel technology for preventing mtDNA transmission from oocytes to pre-implantation embryos...
April 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28369516/comprehensive-chromosome-screening-and-gene-expression-analysis-from-the-same-biopsy-in-human-preimplantation-embryos
#20
Diego Marin, Yujue Wang, Xin Tao, Richard T Scott, Nathan R Treff
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same biopsy of preimplantation human embryos? SUMMARY ANSWER: For the first time, CCS and reliable gene expression analysis have been performed on the same human preimplantation embryo biopsy. WHAT IS KNOWN ALREADY: A single trophectoderm (TE) biopsy is routinely used for many IVF programs offering CCS for selection of only chromosomally normal embryos for transfer...
May 1, 2017: Molecular Human Reproduction
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