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IVF Blastocyst Transfer

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https://www.readbyqxmd.com/read/29047006/extended-culture-of-poor-quality-supernumerary-embryos-improves-art-outcomes
#1
Amira Sallem, Pietro Santulli, Virginie Barraud-Lange, Nathalie Le Foll, Lucile Ferreux, Chloé Maignien, Mathilde Bourdon, Charles Chapron, Dominique de Ziegler, Jean-Philippe Wolf, Khaled Pocate-Cheriet
PURPOSE: The aims of this study were to investigate the possible benefits of extending the culture of poor-quality day-2 embryos (PQE) versus good-quality embryos (GQE) and to identify factors associated with pregnancy and live birth when transferring frozen-thawed blastocysts originating from GQE and PQE. METHODS: This is a retrospective cohort follow-up study performed between November 2012 and February 2015 at the IVF Laboratory Unit of Cochin University Hospital (Paris, France) including 3108 day-2 supernumerary embryos resulting from 1237 IVF/ICSI cycles...
October 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29040520/clinical-implications-of-mitochondrial-dna-quantification-on-pregnancy-outcomes-a-blinded-prospective-non-selection-study
#2
Elpida Fragouli, Caroline McCaffrey, Krithika Ravichandran, Katharina Spath, James A Grifo, Santiago Munné, Dagan Wells
STUDY QUESTION: Can quantification of mitochondrial DNA (mtDNA) in trophectoderm (TE) biopsy samples provide information concerning the viability of a blastocyst, potentially enhancing embryo selection and improving IVF treatment outcomes? SUMMARY ANSWER: This study demonstrated that euploid blastocysts of good morphology, but with high mtDNA levels had a greatly reduced implantation potential. WHAT IS KNOWN ALREADY: Better methods of embryo selection leading to IVF outcome improvement are necessary, as the transfer of chromosomally normal embryos of high morphological grade cannot guarantee the establishment of an ongoing pregnancy...
September 23, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040519/high-gonadotropin-dosage-does-not-affect-euploidy-and-pregnancy-rates-in-ivf-pgs-cycles-with-single-embryo-transfer
#3
Oleksii O Barash, Mary D Hinckley, Evan M Rosenbluth, Kristen A Ivani, Louis N Weckstein
STUDY QUESTION: Does high gonadotropin dosage affect euploidy and pregnancy rates in PGS cycles with single embryo transfer? SUMMARY ANSWER: High gonadotropin dosage does NOT affect euploidy and pregnancy rates in PGS cycles with single embryo transfer. WHAT IS KNOWN ALREADY: PGS has been proven to be the most effective and reliable method for embryo selection in IVF cycles. Euploidy and blastulation rates decrease significantly with advancing maternal age...
October 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29020783/-corifollitropin-alfa-in-women-stimulated-for-the-first-time-in-in%C3%A2-vitro-fertilization-programme
#4
N Vraná-Mardešićová, J Vobořil, L Melicharová, L Jelínková, Š Vilímová, T Mardešić
OBJECTIVES: To compare results after stimulation with corifollitropin alfa (Elonva) in unselected group of women entering for the first time in in vitro fertilization programme (IVF) with results from Phase III randomized trials with selected groups of women. DESIGN: Prospective study. SETTING: Sanatorium Pronatal, Praha. METHODS: 40 unselected women with adequat ovarian reserve entering for the first time in IVF programme were stimulated with corifollitropin alfa and GnRH antagonists...
2017: Ceská Gynekologie
https://www.readbyqxmd.com/read/28985909/neutral-effect-of-body-mass-index-on-implantation-rate-after-frozen-thawed-blastocyst-transfer
#5
Iris G Insogna, Malinda S Lee, Rebecca M Reimers, Thomas L Toth
OBJECTIVE: To examine the effects of body mass index (BMI) on implantation rate after uniform protocol frozen-thawed blastocyst transfer in women with a homogenous uterine environment. DESIGN: Retrospective cohort study. SETTING: Single IVF clinic at a large academic institution. PATIENT(S): Four hundred sixty-one infertile women treated at a large academic institution from January 2007 to January 2014. INTERVENTION(S): All women underwent standardized slow frozen-thawed blastocyst transfers with good-quality day 5-6 embryos, following an identical hormonal uterine preparation, with comparison groups divided according to BMI category: underweight (<18...
October 3, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28985908/effect-of-the-male-factor-on-the-clinical-outcome-of-intracytoplasmic-sperm-injection-combined-with-preimplantation-aneuploidy-testing-observational-longitudinal-cohort-study-of-1-219-consecutive-cycles
#6
Rossella Mazzilli, Danilo Cimadomo, Alberto Vaiarelli, Antonio Capalbo, Lisa Dovere, Erminia Alviggi, Ludovica Dusi, Carlo Foresta, Francesco Lombardo, Andrea Lenzi, Herman Tournaye, Carlo Alviggi, Laura Rienzi, Filippo Maria Ubaldi
OBJECTIVE: To evaluate the impact of the male factor on the outcomes of intracytoplasmic sperm injection (ICSI) cycles combined with preimplantation genetic testing for aneuploidies (PGT-A). DESIGN: Observational longitudinal cohort study. SETTING: Private in vitro fertilization (IVF) center. PATIENT(S): A total of 1,219 oocyte retrievals divided into five study groups according to sperm parameters: normozoospermia (N), moderate male factor (MMF), severe oligoasthenoteratozoospermia (OAT-S), obstructive azoospermia (OA), and nonobstructive azoospermia (NOA)...
October 3, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28939988/frozen-embryo-transfer-can-be-performed-in-the-cycle-immediately-following-the-freeze-all-cycle
#7
Kemal Ozgur, Hasan Bulut, Murat Berkkanoglu, Peter Humaidan, Kevin Coetzee
PURPOSE: In this study, we investigated whether the time interval between oocyte retrieval and frozen embryo transfer (FET) affected the live birth (LB) rates of human segmented-IVF cycles. METHOD: A total of 1338 ICSI freeze-all cycles were performed between February 2015 and January 2016, with 1121 FET cycles being retrospectively analyzed. All vitrified-warmed blastocyst transfers were performed in artificial FET cycles, using gonadotropin-releasing hormone (GnRH) agonist downregulation and oral estrogen endometrial preparation...
September 22, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#8
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#9
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28894983/earlier-day-of-blastocyst-development-is-predictive-of-embryonic-euploidy-across-all-ages-essential-data-for-physician-decision-making-and-counseling-patients
#10
Amy Kaing, Lindsay L Kroener, Robyn Tassin, Man Li, Lian Liu, Richard Buyalos, Gary Hubert, Mousa Shamonki
PURPOSE: The purpose of this study is to evaluate whether day of blastocyst development is associated with embryo chromosomal status as determined by high-density oligonucleotide microarray comparative genomic hybridization (aCGH). METHODS: This is a retrospective cohort analysis, including women who underwent in vitro fertilization (IVF) with trophectoderm biopsy at a single private fertility center from January 2014 to December 2014. Repeat cycles were excluded...
September 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28890427/blastocyst-transfer-does-not-improve-cycle-outcome-as-compared-to-d3-transfer-in-antagonist-cycles-with-an-elevated-progesterone-level-on-the-day-of-hcg
#11
Cem Demirel, Serkan Aydoğdu, Arzu İlknur Özdemir, Gülşah Keskin, Ercan Baştu, Faruk Buyru
OBJECTIVE: To evaluate the association between progesterone elevation on the day of human chorionic gonadotropin (hCG) administration and clinical pregnancy rates of gonadotropin-releasing hormone (GnRH) antagonist in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles with the transfer of embryos at different developmental stages (day-3 versus day-5 ETs). MATERIAL AND METHODS: This is a retrospective analysis of fresh IVF/ICSI; 194 cycles out of 2676 conducted in a single center...
September 1, 2017: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/28887104/should-we-consider-day-2-and-day-3-embryo-morphology-before-day-5-transfer-when-blastocysts-reach-a-similar-good-quality
#12
Charlene Herbemont, Solmaz Sarandi, Jeremy Boujenah, Isabelle Cedrin-Durnerin, Nathalie Sermondade, Alexandre Vivot, Christophe Poncelet, Michael Grynberg, Christophe Sifer
Clinical outcomes of 291 day-5 blastocyst transfers carried out between January 2012 and March 2016 were retrospectively compared according to their quality at day 2 and 3. Inclusion criteria were female age younger than 37 years; first or second IVF and intracytoplasmic sperm injection cycle; quality of the transferred blastocyst: blastocoele B3 or higher; inner-cell-mass A/B; trophectoderm A/B; and known implantation outcome for each transferred blastocyst. Blastocysts were classified into good-quality and poor-quality embryo groups at day 2 and 3...
August 4, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28863936/analysis-of-the-morphological-dynamics-of-blastocysts-after-vitrification-warming-defining-new-predictive-variables-of-implantation
#13
Aila Coello, Marcos Meseguer, Arancha Galán, Lucia Alegre, José Remohí, Ana Cobo
OBJECTIVE: To describe the morphological dynamics of vitrified/warmed blastocysts and to identify quantitative morphological variables related to implantation. Subsequently, by using the most predictive parameters, to develop a hierarchical model by subdividing vitrified/warmed blastocysts into categories with different implantation potentials. DESIGN: Observational, retrospective, cohort study. SETTING: University-affiliated private IVF center...
October 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28859279/the-superovulated-environment-independent-of-embryo-vitrification-results-in-low-birthweight-in-a-mouse-model
#14
Rachel Weinerman, Teri Ord, Marisa S Bartolomei, Christos Coutifaris, Monica Mainigi
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than babies born following frozen embryo transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model that isolates the independent effects of embryo freezing and the superovulated environment, which cannot be performed in humans. We transferred blastocysts that had been vitrified and warmed, mixed with with fresh blastocysts, into individual pseudopregnant recipients produced by either natural mating or mating following injection with equine chorionic gonadotropin and human chorionic gonadotropin and hCG (superovulation)...
July 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28839144/the-sex-ratio-of-singleton-and-twin-delivery-offspring-in-assisted-reproductive-technology-in-china
#15
Mengxi Chen, Jiangbo Du, Jing Zhao, Hong Lv, Yifeng Wang, XiaoJiao Chen, Junqiang Zhang, Lingmin Hu, Guangfu Jin, Hongbing Shen, Zhibin Hu, Fang Xiong, Li Chen, Xiufeng Ling
In order to evaluate the impact of assisted reproductive technology (ART) procedure and individual factors on the sex ratio of singletons and twins at birth after in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI) treatment in China. We conducted a retrospective cohort study including patients undergoing their first cycle of IVF or ICSI with autologous oocytes from 2001 to 2015. A total of 7410 babies were born from 5832 women with 7410 live birth. The secondary sex ratio (SSR) in singletons was significantly higher than twins (53...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28837031/can-ovarian-double-stimulation-in-the-same-menstrual-cycle-improve-ivf-outcomes
#16
Maria Cecília de Almeida Cardoso, Alessandra Evangelista, Cássio Sartório, George Vaz, Caio Luis Vieira Werneck, Fernando Marques Guimarães, Paulo Gallo de Sá, Maria Cecília Erthal
OBJECTIVE: To evaluate the double-stimulation protocol efficacy over conventional ovarian stimulation in recovering a more adequate number of oocytes and increase the number of embryos to be transferred or to be genetically analyzed. METHODS: A retrospective and comparative study with 13 patients who underwent unsuccessful in vitro fertilization (IVF) cycles with a conventional antagonist ovarian stimulation protocol and repeat the attempt with a double stimulation protocol...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28826601/significant-correlation-between-anti-m%C3%A3-llerian-hormone-and-embryo-euploidy-in-a-subpopulation-of-infertile-patients
#17
Itai Gat, Basheer AlKudmani, Karen Wong, Khaled Zohni, Noga Fuchs Weizman, Clifford Librach, Prati Sharma
Anti-Müllerian hormone (AMH) is a standard marker of ovarian reserve. Correlation between AMH and egg euploidy is controversial. We evaluated the association between AMH and blastocyst euploidy rate examined by pre-implantation genetic screening (PGS). This retrospective study was conducted at the CReATe Fertility Centre. We included single IVF cycles of 216 infertile couples, which resulted in 911 blastocysts subjected to array comparative genomic hybridization and evaluated IVF outcome after embryo transfer...
August 1, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#18
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822715/reproductive-outcomes-of-segmented-in-vitro-fertilization-in-patients-diagnosed-with-endometriomas
#19
Kemal Ozgur, Hasan Bulut, Murat Berkkanoglu, Kevin Coetzee
STUDY OBJECTIVE: To assess the impact of ovarian endometriomas on endometrial receptivity in frozen embryo transfer (FET) of segmented in vitro fertilization (IVF) cycles. DESIGN: Retrospective, matched-control study (Canadian Task Force classification II-2). SETTING: A single, private assisted-reproduction technology center. PATIENTS: Thirty patients diagnosed with unilateral or bilateral endometriomas were compared with 60 patients without endometriomas in a population of 1894 patients who underwent segmented IVF treatment between September 2014 and September 2016...
August 16, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28810691/improvement-of-implantation-potential-in-mouse-blastocysts-derived-from-ivf-by-combined-treatment-with-prolactin-epidermal-growth-factor-and-4-hydroxyestradiol
#20
Miki Takeuchi, Misato Seki, Etsuko Furukawa, Akihito Takahashi, Kyosuke Saito, Mitsuru Kobayashi, Kenji Ezoe, Emiko Fukui, Midori Yoshizawa, Hiromichi Matsumoto
STUDY QUESTION: Can supplementation of medium with prolactin (PRL), epidermal growth factor (EGF) and 4-hydroxyestradiol (4-OH-E2) prior to embryo transfer improve implantation potential in mouse blastocysts derived from IVF? SUMMARY ANSWER: Combined treatment with PRL, EGF and 4-OH-E2 improves mouse blastocyst implantation rates, while alone, each factor is ineffective. WHAT IS KNOWN ALREADY: Blastocyst dormancy during delayed implantation caused by ovariectomy is maintained by continued progesterone treatment in mice, and estrogen injection rapidly activates blastocysts to implantation-induced status in vivo...
August 1, 2017: Molecular Human Reproduction
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