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IVF Blastocyst Transfer

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https://www.readbyqxmd.com/read/29458903/day-4-good-morula-embryo-transfer-provided-compatible-live-birth-rate-with-day-5-blastocyst-embryo-in-fresh-ivf-et-cycles
#1
Ryh-Sheng Li, Yuh-Ming Hwu, Robert Kuo-Kuang Lee, Sheng-Hsiang Li, Ming-Huei Lin
OBJECTIVE: Embryo transfers during cleavage stage (day 2 or day 3) and blastocyst stages (day 5 or day 6) are common in current daily practice in fresh IVF/ET cycles. Data regarding transferring day 4 embryos, morula/compact stage, is still restricted and the grading system is also inconsistent, as between IVF clinics. This study provided a new detailed classification system for morula/compact stage embryos and compared successes rates between day 4 and day 5 ET. MATERIALS AND METHODS: This was a retrospective study...
February 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29453221/h3k9-demethylase-kdm4e-is-an-epigenetic-regulator-for-bovine-embryonic-development-and-a-defective-factor-for-nuclear-reprogramming
#2
Xin Liu, Yizhi Wang, Yuanpeng Gao, Jianmin Su, Jingcheng Zhang, Xupeng Xing, Chuan Zhou, Kezhen Yao, Quanli An, Yong Zhang
Aberrant epigenetic reprogramming often results in developmental defects in somatic cell nuclear transfer (SCNT) embryos during embryonic genome activation (EGA). Bovine eight-cell SCNT embryos exhibit global hypermethylation of histone H3 lysine 9 tri- and di-methylation (H3K9me3/2), but the intrinsic reason for this remains elusive. Here, we provide evidence that two H3K9 demethylase genes, lysine-specific demethylase 4D ( KDM4D ) and 4E ( KDM4E ), are related to active H3K9me3/2 demethylation in in vitro fertilized (IVF) embryos and are deficiently expressed in cloned embryos at the time of EGA...
February 16, 2018: Development
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#3
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
https://www.readbyqxmd.com/read/29436143/infertility-treatment-strategy-involving-combined-freeze-all-embryos-and-single-vitrified-warmed-embryo-transfer-during-hormonal-replacement-cycle-for-in-vitro-fertilization-of-women-with-hypogonadotropic-hypogonadism
#4
Keiji Kuroda, Kenji Ezoe, Keiichi Kato, Akiko Yabuuchi, Tomoya Segawa, Tamotsu Kobayashi, Asako Ochiai, Noriko Katoh, Satoru Takeda
AIM: Hypogonadotropic hypogonadism (HH) is a condition caused by the deficient secretion of pituitary gonadotropins, leading to diminished ovarian function. Several studies of in vitro fertilization (IVF) in women with HH revealed acceptable clinical pregnancy outcomes but high multiple pregnancy rates after multiple fresh embryo transfer (ET). The purpose of this study was to analyze the outcomes of combined freeze-all embryos and single vitrified-warmed ET in women with HH. METHODS: Of 91 infertile women with HH (basal luteinizing hormone and follicle-stimulating hormone levels <2...
February 13, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29428306/cumulus-cell-transcriptome-profiling-is-not-predictive-of-live-birth-after-in%C3%A2-vitro-fertilization-a-paired-analysis-of-euploid-sibling-blastocysts
#5
Katherine A Green, Jason M Franasiak, Marie D Werner, Xin Tao, Jessica N Landis, Richard T Scott, Nathan R Treff
OBJECTIVE: To compare the transcriptome of cumulus cells associated with a euploid embryo that resulted in live birth with that of a sibling euploid embryo without sustained implantation. DESIGN: Paired analysis. SETTING: Academic institution. PATIENT(S): Couples undergoing IVF/intracytoplasmic sperm injection with preimplantation genetic screening with female age ≤42 years and normal ovarian reserve. INTERVENTION(S): Transcriptome profiling of cumulus cells from sibling oocytes for correlation with live birth after euploid blastocyst transfer...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29423789/do-trained-reproductive-endocrinologists-perform-better-than-their-trainees-comparing-clinical-pregnancy-rates-and-live-birth-rates-after-transfer-of-single-fresh-blastocysts
#6
Sadikah Behbehani, Joseph Hasson, Stefano Polesello, W Y Son, Togas Tulandi, William Buckett
PURPOSE: To compare clinical pregnancy rates and live birth rates of single blastocyst transfers performed by attending physicians or fellows in reproductive endocrinology and infertility program. METHODS: Retrospective study in an academic reproductive center. We evaluated 932 fresh single blastocyst transfer cycles performed by fellows in training (389 embryo transfers) and by attending physicians (543 embryo transfers). RESULTS: There were no differences in the baseline characteristics and IVF cycle parameters between patients who had transfers performed by fellows or attending physicians...
February 9, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29402277/live-birth-after-laser-assisted-viability-assessment-lava-to-detect-pentoxifylline-resistant-ejaculated-immotile-spermatozoa-during-icsi-in-a-couple-with-male-kartagener-s-syndrome
#7
Sinan Ozkavukcu, Ciler Celik-Ozenci, Esma Konuk, Cem Atabekoglu
Primary ciliary dyskinesia (PCD) is a rare, autosomal recessive disease with abnormalities in the structure of cilia, causing impairment of muco-ciliary clearance with respiratory tract infections, heterotaxia and abnormal sperm motility with male infertility. Here, with a comprehensive literature review, we report a couple with an infertility history of 9 years and three unsuccessful IVF treatments, where male partner has Kartagener's Syndrome, a subtype of PCD, displaying recurrent respiratory infections, dextrocardia and total asthenozoospermia...
February 5, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29394365/live-birth-rate-following-frozen-thawed-blastocyst-transfer-is-higher-with-blastocysts-expanded-on-day-5-than-on-day-6
#8
Lucile Ferreux, Mathilde Bourdon, Amira Sallem, Pietro Santulli, Virginie Barraud-Lange, Nathalie Le Foll, Chloé Maignien, Charles Chapron, Dominique de Ziegler, Jean-Philippe Wolf, Khaled Pocate-Cheriet
STUDY QUESTION: The aim of this study was to evaluate the live birth rate (LBR) after frozen-thawed Day 5 (D5) and Day 6 (D6) blastocyst transfers. SUMMARY ANSWER: LBR following frozen-thawed blastocyst transfer is significantly lower with D6 than with D5 blastocyst regardless of embryo quality. WHAT IS KNOWN ALREADY: During fresh embryo transfer cycles, pregnancy rates (PR) are significantly higher when transferring blastocysts expanded on D5 compared with slow developing blastocysts (D6)...
February 2, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29372155/pre-implantation-genetic-diagnosis-and-pre-implantation-genetic-screening-two-years-experience-at-a-single-center
#9
Se Yeon Won, Hannah Kim, Woo Sik Lee, Ji Won Kim, Sung Han Shim
Objective: Indications for preimplantation genetic diagnosis (PGD)/preimplantation genetic screening (PGS) cycles and clinical outcomes were evaluated at CHA Gangnam Medical Center. Methods: This is retrospective cohort study. All patients (n=336) who went through in vitro fertilization (IVF)-PGD/PGS cycles (n=486) between January 2014 and December 2015 were included in Fertility Center of CHA Gangnam Medical Center. Patients underwent IVF-PGD/PGS with 24-chromosome screening...
January 2018: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/29369190/endometrial-thickness-as-a-predictor-of-the-reproductive-outcomes-in-fresh-and-frozen-embryo-transfer-cycles-a-retrospective-cohort-study-of-1512-ivf-cycles-with-morphologically-good-quality-blastocyst
#10
Tao Zhang, Zhou Li, Xinling Ren, Bo Huang, Guijin Zhu, Wei Yang, Lei Jin
To evaluate the relationship between endometrial thickness during fresh in vitro fertilization (IVF) cycles and the clinical outcomes of subsequent frozen embryo transfer (FET) cycles.FET cycles using at least one morphological good-quality blastocyst conducted between 2012 and 2013 at a university-based reproductive center were reviewed retrospectively. Endometrial ultrasonographic characteristics were recorded both on the oocyte retrieval day and on the day of progesterone supplementation in FET cycles. Clinical pregnancy rate, spontaneous abortion rate, and live birth rate were analyzed...
January 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29353513/effects-of-different-open-cryo-carriers-on-embryo-survival-and-clinical-outcome-in-frozen-embryo-transfer-cycle-patients
#11
Dapeng Chu, Lei Fu, Wenhui Zhou, Yuan Li
The purpose of this study was to compare the efficacy of different open cryo-carriers: the CryoloopTM, CryotopTM, and CryoleafTM, in embryo survival and clinical outcome in patients with frozen embryo transfer (FET) cycle. We analyzed the embryo survival rate and clinical outcome in 325 patients of 348 FET cycles vitrified with the CryoloopTM (160 cycles), CryotopTM (105 cycles), or CryoleafTM (83 cycles). No significant differences were observed in embryo survival rate (98.8% vs. 100% vs. 97.7%, p > 0...
January 20, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29345310/a-potential-role-of-knockout-serum-replacement-as-a-porcine-follicular-fluid-substitute-for-in-vitro-maturation-lipid-metabolism-approach
#12
Jun-Xue Jin, Sanghoon Lee, Erif Maha Nugraha Setyawan, Anukul Taweechaipaisankul, Geon A Kim, Ho Jae Han, Curie Ahn, Byeong Chun Lee
The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. Knockout serum replacement (KSR) is a synthetic protein source, without any undefined growth factors or differentiation-promoting factors. Therefore, it is feasible to use KSR as a defined component for avoiding effects of unknown molecules in an IVM system...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29338137/morphological-embryo-selection-an-elective-single-embryo-transfer-proposal
#13
Francisco Parera Déniz, Carlos Encinas, Jorge La Fuente
OBJECTIVE: To describe a patient selection method for elective single embryo transfer (eSET), emphasizing inclusion criteria and results. METHODS: This retrospective study included all cases seen in a private clinic between June 2011 and December 2016, in La Paz, Bolivia (3600 meters above sea level). Elective single embryo transfer was the method of choice in 34 IVF/ICSI cycles, all in the blastocyst stage. Gardner's blastocyst classification criteria were used...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29322346/study-on-the-incidence-and-influences-on-heterotopic-pregnancy-from-embryo-transfer-of-fresh-cycles-and-frozen-thawed-cycles
#14
Shan Xiao, Meilan Mo, Xiaodong Hu, Hongzhan Zhang, Shiru Xu, Zhuran Wang, Yong Zeng
PURPOSE: The purpose of this study was to determine the heterotopic pregnancy rate using fresh versus frozen-thawed embryo transfers and factors associated with heterotopic pregnancy (HP). Management and clinical outcomes after heterotopic pregnancy were also evaluated. METHODS: In this retrospective cohort study, we included 12,484 women who had clinical pregnancies after in vitro fertilization treatment at our fertility center between 2012 and 2017. Patients received fresh day 3 embryos (F-D3 group), fresh day 5 blastocysts (F-D5 group), frozen-thawed day 3 embryos (T-D3 group), or frozen-thawed day 5 or 6 blastocysts (T-D5/6 groups) transfers...
January 11, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29297113/frozen-blastocyst-transfer-outcomes-in-immediate-versus-delayed-subsequent-cycles-following-gnrh-agonist-or-hcg-triggers
#15
Leah Kaye, Audrey Marsidi, Puja Rai, Jeffrey Thorne, John Nulsen, Lawrence Engmann, Claudio Benadiva
PURPOSE: The aim of this study is to analyze clinical pregnancy rates (CPR) and ongoing pregnancy rates (OPR) for frozen embryo transfers (FET) performed with blastocysts in the cycle immediately after GnRH agonist (GnRHa) versus human chorionic gonadotropin (hCG) triggers, with outcomes of delayed FET for comparison. METHODS: Retrospective cohort study at a university-affiliated in vitro fertilization (IVF) clinic, including patients undergoing IVF between 2013-16 with a blastocyst FET performed within two menstrual cycles of a previous stimulation cycle and vaginal oocyte retrieval (VOR)...
January 3, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29279182/elective-frozen-thawed-embryo-transfer-fet-in-women-at-risk-for-ovarian-hyperstimulation-syndrome
#16
J Zech, A Brandao, M Zech, K Lugger, S Neururer, H Ulmer, E Ruttmann
Elective cryopreservation of cultured embryos has become a treatment option for women at risk for ovarian hyperstimulation syndrome (OHSS). The aim of our study was to investigate the outcome of elective cryopreservation and consecutive frozen-thawed embryo transfer (FET) in a large IVF clinic in Austria. A total of 6104 controlled ovarian hyperstimulation cycles (COH) were performed on 2998 patients including 200 patients (6.7%) who were undergoing elective cryopreservation and FET due to high risk of OHSS...
December 24, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29259463/pregnancy-outcomes-after-assisted-reproductive-procedures-with-embryos-that-had-been-derived-from-affected-and-unaffected-ovaries-among-women-with-small-unilateral-endometriomas
#17
Akiko Takashima, Naoki Takeshita, Toshihiko Kinoshita
Aim: To clarify the effects of small endometriomas on in vitro fertilization (IVF) outcomes. In the present study, the potential impact of small ovarian endometriomas on the quantitative and qualitative outcomes of IVF was evaluated in the same individual. Methods: A retrospective analysis was performed, in which 118 infertile women with unilateral endometriomas that were <40 mm in size and who underwent IVF or intracytoplasmic sperm injection were evaluated...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29246561/successful-quintuplet-pregnancy-of-monochorionic-male-quadruplets-and-single-female-after-double-embryo-transfer-case-report-and-review-of-the-literature
#18
Ryan Schlueter, Christina Arnett, Christopher Huang, Janet Burlingame
OBJECTIVE: To report a rare case of quintuplets with monochorionic male quadruplets and a single female after two-blastocyst transfer. DESIGN: Case report. SETTING: University teaching hospital. PATIENT(S): A 37-year-old gravida 1, para 1, presenting after two-embryo IVF with monochorionic male quadruplets and a single female. INTERVENTION(S): Ultrasound examinations and cesarean delivery. MAIN OUTCOME MEASURE(S): Successful delivery of five live-born infants at 28 weeks and 6 days of gestation for preterm labor...
December 12, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/29243796/can-high-levels-of-d-chiro-inositol-in-follicular-fluid-exert-detrimental-effects-on-blastocyst-quality
#19
K Ravanos, G Monastra, T Pavlidou, M Goudakou, N Prapas
OBJECTIVE: It was previously shown that higher concentrations of myo-inositol in human follicular fluid improve oocyte and embryo quality, whereas D-chiro-inositol seems to worsen oocyte quality and ovarian response in polycystic ovary syndrome patients. Our study was the first one aiming to test whether different myo-inositol and D-chiro-inositol concentration in follicular fluids correlate with blastocyst quality in healthy young women. PATIENTS AND METHODS: Eight egg donors and eleven couples undergoing in vitro fertilization, were involved in a prospective observational study...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29205617/epigenetic-states-of-donor-cells-significantly-affect-the-development-of-somatic-cell-nuclear-transfer-scnt-embryos-in-pigs
#20
Yanhui Zhai, Wei Li, Zhiren Zhang, Yunqing Cao, Zhengzhu Wang, Sheng Zhang, Ziyi Li
The type and pattern of epigenetic modification in donor cells can significantly affect the developmental competency of somatic cell nuclear transfer (SCNT) embryos. Here, we investigated the developmental capacity, gene expression, and epigenetic modifications of SCNT embryos derived from porcine bone marrow-derived mesenchymal stem cells (BMSCs) and fetal fibroblasts (FFs) donor cells compared to embryos obtained from in vitro fertilization (IVF). Compared to FFs, the donor BMSCs had more active epigenetic markers (Histone H3 modifications: H3K9Ac, H3K4me3, and H3K4me2) and fewer repressive epigenetic markers (H3K9me3, H3K9me2, and DNA methyltransferase 1)...
December 4, 2017: Molecular Reproduction and Development
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