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IVF Blastocyst Transfer

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https://www.readbyqxmd.com/read/27918161/-current-options-of-preimplantion-genetic-screening-and-preimplantation-genetic-diagnostics
#1
V Šimečková
OBJECTIVE: The aim of this work is to summarize the current knowledge about preimplantation genetic screening and diagnostics. DESIGN: A review article. SETTING: Department of Gynecology and Obstetrics, District Hospital Šternberk, IVF Clinic, Olomouc. RESULTS: Preimplantation genetic testing is a complex of genetic and molecular cytogenetic examinations, which can help to detect abnormalities in embryos before transfer into the uterus of the mother...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27894197/temporal-validation-of-a-simplified-blastocyst-grading-system
#2
Alison Richardson, Sophie Brearley, Saran Ahitan, Sarah Chamberlain, Tracey Davey, Lyndsey Zujovic, James Hopkisson, Bruce Campbell, Nick Raine-Fenning
We have recently established the clinical effectiveness and credibility of a simplified blastocyst grading system by demonstrating its prognostic potential and the inter- and intra-observer variability associated with it. To be considered clinically useful, however, the grading system also needs to be accurate (i.e. well calibrated with good discriminative ability). This study prospectively evaluates the performance of the grading system on subsequent patients from the same IVF unit in an attempt to temporally validate the model...
November 28, 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27874292/granulocyte-colony-stimulating-factor-treatment-of-resistant-thin-endometrium-in-women-with-frozen-thawed-blastocyst-transfer
#3
Michał Kunicki, Krzysztof Łukaszuk, Joanna Liss, Patrycja Skowrońska, Joanna Szczyptańska
: The aim of the study was to assess the granulocyte-colony stimulating factor (G-CSF) effect on unresponsive thin (<7 mm) endometrium in women undergoing frozen-thawed embryo transfer at the blastocyst stage. A total of 62 women with thin unresponsive endometrium were included in the study, of which, 29 received a G-CSF infusion and 33 who opted out of the study served as controls. Patients in both groups had similar endometrial thickness at the time of the initial evaluation: 6.50 mm (5...
November 22, 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27852688/the-role-of-intracytoplasmic-sperm-injection-in-non-male-factor-infertility-in-advanced-maternal-age
#4
Samer Tannus, Weon-Young Son, Ashley Gilman, Grace Younes, Tal Shavit, Michael-Haim Dahan
STUDY QUESTION: Does ICSI improve reproductive outcomes compared with conventional IVF when used for non-male factor infertility in women aged 40 years and over? SUMMARY ANSWER: There is no advantage of ICSI over conventional IVF in women aged 40 years and over when used for non-male factor infertility. WHAT IS KNOWN ALREADY: The use of ICSI has increased dramatically in recent years and is being applied for indications other than male factor infertility...
November 16, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27852632/predicting-the-chances-of-a-live-birth-after-one-or-more-complete-cycles-of-in-vitro-fertilisation-population-based-study-of-linked-cycle-data-from-113%C3%A2-873-women
#5
David J McLernon, Ewout W Steyerberg, Egbert R Te Velde, Amanda J Lee, Siladitya Bhattacharya
OBJECTIVE:  To develop a prediction model to estimate the chances of a live birth over multiple complete cycles of in vitro fertilisation (IVF) based on a couple's specific characteristics and treatment information. DESIGN:  Population based cohort study. SETTING:  All licensed IVF clinics in the UK. National data from the Human Fertilisation and Embryology Authority register. PARTICIPANTS:  All 253 417 women who started IVF (including intracytoplasmic sperm injection) treatment in the UK from 1999 to 2008 using their own eggs and partner's sperm...
November 16, 2016: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/27827818/oocyte-embryo-and-blastocyst-cryopreservation-in-art-systematic-review-and-meta-analysis-comparing-slow-freezing-versus-vitrification-to-produce-evidence-for-the-development-of-global-guidance
#6
REVIEW
Laura Rienzi, Clarisa Gracia, Roberta Maggiulli, Andrew R LaBarbera, Daniel J Kaser, Filippo M Ubaldi, Sheryl Vanderpoel, Catherine Racowsky
BACKGROUND: Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular dehydration while minimizing intracellular ice formation. Vitrification allows the solidification of the cell(s) and of the extracellular milieu into a glass-like state without the formation of ice...
November 8, 2016: Human Reproduction Update
https://www.readbyqxmd.com/read/27759446/ectopic-pregnancy-following-in-vitro-fertilization-meta-analysis-and-single-center-experience-during-6-years
#7
V Muller, M Makhmadalieva, I Kogan, I Fedorova, E Lesik, E Komarova, L Dzhemlikhanova, D Niauri, A Gzgzyan, E Ailamazyan
BACKGROUND: Ectopic pregnancy (EP) has been reported to occur in 1.4-5.4% of all clinical pregnancies resulting from in vitro fertilization (IVF) and embryo transfer (ET). Data on factors associated with abnormal embryo implantation following assisted conception are limited. MATERIALS AND METHODS: A systematic review and meta-analysis was performed to determine whether there is an association between the day (cleavage-stage, D3, versus blastocyst, D5) or the type (fresh versus frozen/thawed) of ET and EP rate...
October 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27746110/a-direct-healthcare-cost-analysis-of-the-cryopreserved-versus-fresh-transfer-policy-at-the-blastocyst-stage
#8
Enrico Papaleo, Luca Pagliardini, Valeria Stella Vanni, Diana Delprato, Patrizia Rubino, Massimo Candiani, Paola Viganò
A cost analysis covering direct healthcare costs relating to IVF freeze-all policy was conducted. Normal- and high- responder patients treated with a freeze-all policy (n = 63) compared with fresh transfer IVF (n = 189) matched by age, body mass index, duration and cause of infertility, predictive factors for IVF (number of oocytes used for fertilization) and study period, according to a 1:3 ratio were included. Total costs per patient (€6952 versus €6863) and mean costs per live birth were similar between the freeze-all strategy (€13,101, 95% CI 10,686 to 17,041) and fresh transfer IVF (€15,279, 95% CI 13,212 to 18,030)...
October 3, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27743693/oocytes-with-smooth-endoplasmic-reticulum-clusters-originate-blastocysts-with-impaired-implantation-potential
#9
Amanda Souza Setti, Rita Cássia Sávio Figueira, Daniela Paes de Almeida Ferreira Braga, Matheus de Castro Azevedo, Assumpto Iaconelli, Edson Borges
OBJECTIVE: To study whether embryos derived from oocytes presenting a smooth endoplasmic reticulum cluster (SERC) are less likely to develop into blastocysts and implant. DESIGN: Transversal study. SETTING: Private university-affiliated in vitro fertilization (IVF) center. PATIENT(S): Total of 7,609 oocytes obtained from 743 intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Oocytes split between the SERC-positive cycles (with at least one SERC-positive oocyte) and the SERC-negative cycles (only oocytes free of SERC)...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27731533/blastocyst-versus-cleavage-stage-embryo-transfer-a-systematic-review-and-meta-analysis-of-the-reproductive-outcomes
#10
W P Martins, C O Nastri, L Rienzi, S Z van der Poel, C Gracia, C Racowsky
OBJECTIVES: Blastocyst transfer could be advantageous because it allows a more physiologic timing of exposure of the embryo to the uterine environment and permit embryo self-selection after activation of the embryonic genome on day 3. Conversely, the in vitro environment is probably inferior to that provided in vivo and in vitro culture beyond embryonic genomic activation could potentially harm embryos. Our objective was to identify, appraise and summarize the available evidence comparing the effectiveness of blastocyst and cleavage stage embryo transfer...
October 12, 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27689039/clinical-outcomes-of-single-versus-double-blastocyst-transfer-in-fresh-and-vitrified-warmed-cycles
#11
Jin Hee Eum, Jae Kyun Park, So Young Kim, Soo Kyung Paek, Hyun Ha Seok, Eun Mi Chang, Dong Ryul Lee, Woo Sik Lee
OBJECTIVE: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27682145/pre-implantation-genetic-screening-techniques-implications-for-clinical-prenatal-diagnosis
#12
Stefan C Kane, Elissa Willats, Sammya Bezerra Maia E Holanda Moura, Jonathan Hyett, Fabrício da Silva Costa
Chromosomal aneuploidy is responsible for a significant proportion of pregnancy failures, whether conceived naturally or through in vitro fertilization (IVF). In an effort to improve the success rate of IVF, screening embryos for aneuploidy - or pre-implantation genetic screening (PGS) - has been proposed as a means of ensuring only euploid embryos are selected for transfer. Early PGS approaches were based on fluorescence in situ hybridization testing, and have been shown not to improve live birth rates. Recent developments in genetic testing technologies - such as next-generation sequencing and quantitative polymerase chain reaction, coupled with embryo biopsy at the blastocyst stage - have shown promise in improving IVF outcomes, but they remain to be validated in adequately powered, prospective randomized trials...
September 29, 2016: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/27678033/addition-of-neither-recombinant-nor-urinary-luteinizing-hormone-was-associated-with-an-improvement-in-the-outcome-of-autologous-in%C3%A2-vitro-fertilization-intracytoplasmatic-sperm-injection-cycles-under-regular-clinical-settings-a-multicenter-observational-analysis
#13
Juan-Enrique Schwarze, Javier A Crosby, Fernando Zegers-Hochschild
OBJECTIVE: To determine whether the addition of either urinary or recombinant LH in patients undergoing routine clinical care improved the outcome in terms of the number of oocytes recovered for insemination or the delivery rate per initiated cycle. DESIGN: Cohort analysis. PATIENT(S): Couples undergoing IVF/ICSI in 158 institutions in 15 countries in Latin America. SETTING: In vitro fertilization clinics. INTERVENTION(S): We compared the outcome of three different protocols of COH, including rFSH only, rFSH plus rLH, and rFSH plus hMG...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27664207/population-trends-and-live-birth-rates-associated-with-common-art-treatment-strategies
#14
Georgina M Chambers, Handan Wand, Alan Macaldowie, Michael G Chapman, Cynthia M Farquhar, Mark Bowman, David Molloy, William Ledger
STUDY QUESTION: Have ART live birth rates improved in Australia over the last 12 years? SUMMARY ANSWER: There were striking improvements in per-cycle live birth rates observed for frozen/thaw embryo transfers, blastocyst transfer and single embryo transfer (SET), while live birth rates following ICSI were lower than IVF for non-male factor infertility in most years. WHAT IS ALREADY KNOWN: ART and associated techniques have become the predominant treatment of infertility over the past 30 years in most developed countries...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27662416/gnrh-agonist-trigger-with-intensive-luteal-phase-support-vs-human-chorionic-gonadotropin-trigger-in-high-responders-an-observational-study-reporting-pregnancy-outcomes-and-incidence-of-ovarian-hyperstimulation-syndrome
#15
Georgios Christopoulos, Antonios Vlismas, Anna Carby, Stuart Lavery, Geoffrey Trew
A retrospective, cohort study of high-risk patients undergoing IVF treatment was performed to assess if there is a difference in clinical pregnancy rate, live birth rate and the incidence of ovarian hyperstimulation syndrome, when a GnRH agonist (GnRHa) trigger with intensive luteal support is compared to human chorionic gonadotropin (hCG) with standard luteal support. The control group consisted of 382 high-risk patients having a GnRH antagonist protocol with 194 receiving an hCG trigger. All patients had ≥18 follicles ≥11mm or serum oestradiol >18,000pmol/l on the day of trigger...
September 2016: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27651522/uterine-focal-adhesions-retained-at-implantation-after-rat-ovarian-hyperstimulation
#16
Laura Lindsay, Samson N Dowland, Christopher R Murphy
Controlled ovarian hyperstimulation is an essential component of IVF techniques to ensure proliferation and development of multiple ovarian follicules but the effects of these hormones on the endometrium are largely unknown. During normal pregnancy in rats, there are significant changes in the basal plasma membrane of uterine epithelial cells (UECs) at the time of receptivity, including loss of focal adhesions. This enables the UECs to be removed from the implantation chamber surrounding the blastocyst, thus allowing invasion into the underlying stroma...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27645282/how-much-have-we-learned-from-time-lapse-in-clinical-ivf
#17
D Castelló, Y Motato, N Basile, J Remohí, M Espejo-Catena, M Meseguer
Can the time-lapse system (TLS) identify the best embryo for transfer? Although there are several studies that support this hypothesis, more research is required to improve the quality of the current evidence and also to assess live birth rate, miscarriage, stillbirth or clinical pregnancy in order to choose between a TLS or conventional incubation. In addition, although some authors report on effectiveness and safety in the use of TLS monitoring of embryo development in vitro, other authors that have not found relevant differences between the two systems for the culture and subsequence embryo selection...
October 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27628754/novel-embryo-selection-techniques-to-increase-embryo-implantation-in-ivf-attempts
#18
George Α Sigalos, Olga Triantafyllidou, Nikos F Vlahos
PURPOSE: The final success of an IVF attempt depends on several steps and decisions taken during the ovarian stimulation, the oocyte retrieval, the embryo culture and the embryo transfer. The final selection of the embryos most likely to implant is the final step in this process and the responsibility of the lab. Apart from strict morphologic criteria that historically have been used in embryo selection, additional information on genetic, metabolomic and morphokinetic characteristics of the embryo is recently combined to morphology to select the embryo most likely to produce a pregnancy...
September 15, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/27619774/embryo-selection-using-time-lapse-analysis-early-embryo-viability-assessment-in-conjunction-with-standard-morphology-a-prospective-two-center-pilot-study
#19
Dorit C Kieslinger, Stefanie De Gheselle, Cornelis B Lambalk, Petra De Sutter, E Hanna Kostelijk, Jos W R Twisk, Joukje van Rijswijk, Etienne Van den Abbeel, Carlijn G Vergouw
STUDY QUESTION: Does prospective embryo selection using the results from the Eava Test (Early Embryo Viability Assessment) in combination with standard morphology increase the pregnancy rate of IVF and ICSI patients compared to embryo selection based on morphology only? SUMMARY ANSWER: Embryo selection using the Eeva Test plus standard morphology on Day 3 results in comparable pregnancy rates as conventional morphological embryo selection. WHAT IS KNOWN ALREADY: Time-lapse monitoring of embryo development may represent a superior way to culture and select embryos in vitro...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27619770/hatching-status-before-embryo-transfer-is-not-correlated-with-implantation-rate-in-chromosomally-screened-blastocysts
#20
Jorge Rodriguez-Purata, Julian Gingold, Joseph Lee, Michael Whitehouse, Richard Slifkin, Christine Briton-Jones, Alan Copperman, Benjamin Sandler
STUDY QUESTION: Do the reproductive outcomes from the transfer of fully hatched (FH) blastocysts differ from those of not fully hatched (NFH) blastocysts? SUMMARY ANSWER: Biochemical pregnancy rate (BPR), implantation rate (IR), live birth rate (LBR) and early pregnancy loss (EPL) rate are similar in FH and NFH single euploid blastocyst embryo transfers. WHAT IS KNOWN ALREADY: The use of extended culture and PGS often leads to transfer of an embryo that is well developed and frequently FH from the zona pellucida...
November 2016: Human Reproduction
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