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Embryo Culture Media

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https://www.readbyqxmd.com/read/28630431/role-of-methionine-adenosyltransferase-2a-in-bovine-preimplantation-development-and-its-associated-genomic-regions
#1
Shuntaro Ikeda, Ryouka Kawahara-Miki, Hisataka Iwata, Miki Sugimoto, Shinichi Kume
Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28617821/supplementation-with-small-extracellular-vesicles-from-ovarian-follicular-fluid-during-in-vitro-production-modulates-bovine-embryo-development
#2
Juliano C da Silveira, Gabriella M Andrade, Maite Del Collado, Rafael V Sampaio, Juliano R Sangalli, Luciano A Silva, Fábio V L Pinaffi, Izabelle B Jardim, Marcelo C Cesar, Marcelo F G Nogueira, Aline S M Cesar, Luiz L Coutinho, Rinaldo W Pereira, Felipe Perecin, Flávio V Meirelles
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception. Alternatives to overcome these problems are based on assisted reproductive techniques. Extracellular vesicles are cell-secreted vesicles present in different body fluids and contain bioactive materials, such as messenger RNA, microRNAs (miRNAs), and proteins. Thus, our hypothesis is that extracellular vesicles from follicular fluid from 3-6 mm ovarian follicles can modulate bovine embryo development in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28610639/impact-of-the-ivf-laboratory-environment-on-human-preimplantation-embryo-phenotype
#3
D K Gardner, R L Kelley
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development...
June 14, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28601148/leptin-supplementation-in%C3%A2-vitro-improved-developmental-competence-of-buffalo-oocytes-and-embryos
#4
Bibhudatta S K Panda, Sriti Pandey, Anjali Somal, Mehtab S Parmar, Irfan A Bhat, Indu Baiju, Mukesh K Bharti, G Sai Kumar, Vikash Chandra, G Taru Sharma
The aim of the present study was to determine potential role of leptin on in vitro developmental competence of buffalo oocytes and embryos. Slaughterhouse derived culture grade buffalo cumulus oocyte complexes (COCs) were matured in vitro (IVM) with leptin (10 ng/ml) or without leptin (control). In each experiment, a pool of matured COCs was used for further in vitro embryo production and another pool of COCs was used for cumulus cells and mature oocytes isolation to study the relative mRNA expression of developmentally important genes...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28592664/altered-retinoid-signaling-compromises-decidualization-in-endometriotic-stromal-cells
#5
Mary Ellen Pavone, Saurabh Malpani, Matthew Dyson, Serdar Bulun
Decidualization alters multiple molecular pathways in endometrium to permit successful embryo implantation. We have reported that paracrine factors, including retinoids, secreted from progesterone treated endometrial stromal cells act on nearby epithelial cells to induce the estradiol metabolizing enzyme HSD17B2. This same induction is not seen in endometriotic stromal cells. We have also shown significant differences in retinoid uptake, metabolism and action in endometriotic tissue and stromal cells compared to normal endometrium...
June 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28583200/high%C3%A2-quality-clinical%C3%A2-grade-human-embryonic-stem-cell-lines-derived-from-fresh-discarded-embryos
#6
Jinpei Ye, Nicola Bates, Despina Soteriou, Lisa Grady, Clare Edmond, Alex Ross, Alan Kerby, Philip A Lewis, Tope Adeniyi, Ronnie Wright, Kay V Poulton, Marcus Lowe, Susan J Kimber, Daniel R Brison
BACKGROUND: Human embryonic stem cells (hESCs) hold tremendous promise for cell replacement therapies for a range of degenerative diseases. In order to provide cost-effective treatments affordable by public health systems, HLA-matched allogeneic tissue banks of the highest quality clinical-grade hESCs will be required. However only a small number of existing hESC lines are suitable for clinical use; they are limited by moral and ethical concerns and none of them apply Good Manufacturing Practice (GMP) standards to the earliest and critical stages of gamete and embryo procurement...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28565768/raman-spectrum-a-potential-biomarker-for-embryo-assessment-during-in-vitro-fertilization
#7
Jiayi Ding, Tian Xu, Xiaofang Tan, Hua Jin, Jun Shao, Haibo Li
The aim of the study was to investigate whether Raman spectrum is consistent with the morphological scoring of the embryo of day 3 during in vitro fertilization (IVF). The spent culture media of embryo of day 3 from 10 patients were collected and analyzed. The samples were analyzed using Raman spectroscopy and graded according to the standard embryo scoring system simultaneously. Data showed that the Raman spectra obtained from the droplet of media were useful, as they can act as the characteristic signature for protein and amino acids...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28558842/natriuretic-peptides-improve-the-developmental-competence-of-in-vitro-cultured-porcine-oocytes
#8
Yanhao Zhang, Huarong Wang, Wei Liu, Ye Yang, Xiao Wang, Zhiyuan Zhang, Qirui Guo, Chao Wang, Guoliang Xia
BACKGROUND: Natriuretic peptides (NPs), brain and C type NPs (BNP and CNP), were involved in the maintenance of porcine oocyte meiotic arrest. The present study investigated the effects of NPs on developmental competence of immature porcine oocytes with follicles of different sizes. METHODS: Follicular fluid NP levels were examined by radioimmunoassay. The developmental competence of porcine oocytes was evaluated by cleavage and blastocyst developmental rates after in vitro fertilization (IVF) or parthenogenetic activation (PA) of cumulus oocyte complexes (COCs), which were recovered from follicle with different sizes...
May 30, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28545340/optimized-methods-for-rapidly-dissecting-spinal-cords-and-harvesting-spinal-motor-neurons-with-high-survival-and-purity-from-rats-at-different-embryonic-stages
#9
Shudong Chen, Ruimin Tian, Hui Li, Meihui Chen, Hu Zhang, Dingkun Lin
STUDY DESIGN: Experimental study, protocol optimization. OBJECTIVES: To investigate and compare the isolation of spinal motor neurons from embryonic rats at different embryonic stages, and develop optimized methods for rapidly dissecting spinal cords and harvesting spinal motor neurons with high survival and purity. SETTING: Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China. METHODS: Embryonic rats at different embryonic stages (12-18 days) were used to isolate spinal motor neurons...
May 25, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/28542562/identification-and-characteristics-of-extracellular-vesicles-from-bovine-blastocysts-produced-in-vitro
#10
Edwin A Mellisho, Alejandra E Velásquez, María J Nuñez, Joel G Cabezas, Juan A Cueto, Claudio Fader, Fidel O Castro, Lleretny Rodríguez-Álvarez
Extracellular vesicles (EVs) have been identified within different body fluids and cell culture media. However, there is very little information on the secretion of these vesicles during early embryonic development. The aims of this work were first to demonstrate the secretion of extracellular vesicles by pre-implantation bovine embryos and second to identify and characterize the population of EVs secreted by bovine blastocysts during the period from day seven to nine of embryo culture and its correlation with further embryo development up to day 11...
2017: PloS One
https://www.readbyqxmd.com/read/28537496/feeder-free-derivation-of-na%C3%A3-ve-human-pluripotent-stem-cells
#11
Emily Ward, Kirk Twaroski, Jakub Tolar
Human pluripotent stem cells (HPSCs) cultured in conditions that maintain pluripotency via FGF and TGFβ signaling have been described as being in a primed state. These cells have been shown to exhibit characteristics more closely related to mouse epiblast-derived stem cells than to so called naïve mouse PSCs said to possess a more ground state pluripotency that mimics the early mouse embryo inner cell mass. Initial attempts to create culture conditions favorable for generation of naïve HPSCs from primed HPSCs has required the use of mouse embryonic fibroblasts as a feeder layer to support this transition...
May 24, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28532595/impact-of-elemental-iron-on-human-spermatozoa-and-mouse-embryonic-development-in-a-defined-synthetic-culture-medium
#12
Safaa M Naes, Oshini Basri, Fauziah Ismail, Ghofraan A Ata'Allah, Siti Khadijah Idris, Noor Azmi Mat Adenan, Jaffar Ali
There is a paucity of studies on effect of iron in embryo culture procedures. This study aims to ascertain the optimal, tolerance and toxic levels of iron in a protein-free embryo culture medium (PFM) to determine the effect of iron on embryonic development. The application of PFM in assisted reproductive technologies (ART) is intended to eliminate disease transmission and improve ART treatment outcome. The optimal, tolerance and toxic levels of iron on human spermatozoa and mouse embryos were determined by challenging them with different levels of iron (ferric iron; Fe(+3))...
May 19, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28526407/sirtuin-inhibition-leads-to-autophagy-and-apoptosis-in-porcine-preimplantation-blastocysts
#13
Min Gyeong Kim, Duk Hyoun Kim, Hye Ran Lee, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. Previous studies have shown that sirtuin inhibition leads to embryonic developmental arrest and oxidative stress in porcine and murine. However, sirtuin-mediated mechanisms have not been examined in porcine preimplantation blastocysts. We therefore investigated the relationship between sirtuins and autophagy. Embryos were cultured with 100 μM sirtinol (SIRT1/2 inhibitor) in NCSU-23 media after in vitro fertilization...
May 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28511995/live-full-term-mouse-pups-from-oocytes-grown-and-matured-in-vitro-with-serum-substitutes
#14
Hideyuki H Motohashi, Ryoma Taniguchi, Junpei Sakamoto, Tadashi Sankai, Hidemi Kada
For in vitro growth and maturation of mouse oocytes (IVG-IVM), serum is added to media up to and including the stage of oocyte maturation; this subsequently supports oocytes through fertilization and early embryo development. However, problems may occur with sera, such as batch differences and issues of biosafety. The purpose of the present study was to determine the capacity for fertilization and pre- and post-implantation development of oocytes that underwent IVG-IVM with a serum substitute. Oocyte-granulosa cell complexes from preantral follicles were cultured in medium with either fetal bovine serum (FBS), Serum Substitute Supplement™ (SSS), or Knockout™ Serum Replacement (KSR) for 10days, and were then allowed to mature for 17 h...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28496018/effects-of-heat-stress-on-bovine-preimplantation-embryos-produced-in-vitro
#15
Miki Sakatani
Summer heat stress decreases the pregnancy rate in cattle and has been thought to be associated with the early embryonic death caused by the elevation of maternal body temperature. In vitro cultures have been widely used for the evaluation of effects of heat stress on oocytes, fertilization, preimplantation, and embryonic development. Susceptibility to heat stress is present in developmental stages from oocytes to cleavage-stage (before embryonic gene activation, EGA) embryos, leading to a consequent decrease in developmental competence...
May 12, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28444601/somatic-embryogenesis-from-bud-and-leaf-explants-of-date-palm-phoenix-dactylifera-l-cv-najda
#16
Mouaad Amine Mazri, Ilham Belkoura, Reda Meziani, Boutaïna Mokhless, Souad Nour
An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. The highest rate of somatic embryogenesis (86.0%) was obtained on bud explants cultured on MS medium supplemented with 45...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28438071/time-to-re-evaluate-art-protocols-in-the-light-of-advances-in-knowledge-about-methylation-and-epigenetics-an-opinion-paper
#17
Yves Menezo, Brian Dale, Kay Elder
DNA methylation is a biochemical process that modifies gene expression without changing the underlying DNA sequence, and this represents the molecular basis for imprinting and epigenetics. Recent reports have revealed alterations in DNA methylation profiles in the placenta of babies born from assisted reproductive technologies (ART). This supports several previous observations that suggested an increase in the prevalence of imprinting diseases following ART treatment, and also fits our observations regarding the metabolism and requirements of early human embryos...
April 25, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28434226/in-situ-microprobe-single-cell-capillary-electrophoresis-mass-spectrometry-metabolic-reorganization-in-single-differentiating-cells-in-the-live-vertebrate-x-laevis-embryo
#18
Rosemary M Onjiko, Erika P Portero, Sally A Moody, Peter Nemes
Knowledge of single-cell metabolism would provide a powerful window to cell activity changes as cells differentiate to all the tissues of the vertebrate embryo. However, single-cell mass spectrometry technologies have not yet been made compatible with complex three-dimensional changes and rapidly decreasing cell sizes during early development of the embryo. Here, we bridge this technological gap by integrating capillary microsampling, microscale metabolite extraction, and capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable direct metabolic metabolic analysis of identified cells in the live frog embryo (Xenopus laevis)...
April 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28421107/sexual-dimorphism-of-mirnas-secreted-by-bovine-in-vitro-produced-embryos
#19
Nicole Gross, Jenna Kropp, Hasan Khatib
Sexual dimorphism of bovine blastocysts has previously been observed through differences in development, cell death, metabolism, telomere length, DNA methylation, and transcriptomics. However, dimorphism in the secretion of miRNAs to culture media has not yet been evaluated. The objectives of this study were to determine if sex-specific blastocyst miRNA secretion occurs and to further investigate the role these miRNAs may have in the interaction between a blastocyst and the maternal environment. In vitro embryo culture was performed and media from male and female blastocysts was collected into sex-specific pools...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28416168/non-invasive-preimplantation-genetic-screening-using-array-comparative-genomic-hybridization-on-spent-culture-media-a-proof-of-concept-pilot-study
#20
Michael Feichtinger, Enrico Vaccari, Luca Carli, Elisabeth Wallner, Ulrike Mädel, Katharina Figl, Simone Palini, Wilfried Feichtinger
The aim of this pilot study was to assess if array comparative genomic hybridization (aCGH), non-invasive preimplantation genetic screening (PGS) on blastocyst culture media is feasible. Therefore, aCGH analysis was carried out on 22 spent blastocyst culture media samples after polar body PGS because of advanced maternal age. All oocytes were fertilized by intracytoplasmic sperm injection and all embryos underwent assisted hatching. Concordance of polar body analysis and culture media genetic results was assessed...
March 28, 2017: Reproductive Biomedicine Online
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