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Embryo Culture Media

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https://www.readbyqxmd.com/read/29325603/culture-conditions-affect-ca2-release-in-artificially-activated-mouse-and-human-oocytes
#1
Yuechao Lu, Davina Bonte, Minerva Ferrer-Buitrago, Mina Popovic, Jitesh Neupane, Margot Van der Jeught, Luc Leybaert, Petra De Sutter, Björn Heindryckx
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM...
January 12, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29301092/metabolomic-profiling-of-bovine-oviductal-fluid-across-the-oestrous-cycle-using-proton-nuclear-magnetic-resonance-spectroscopy
#2
Julie Lamy, Julie Gatien, Florine Dubuisson, Lydie Nadal-Desbarats, Pascal Salvetti, Pascal Mermillod, Marie Saint-Dizier
In the present study we tested whether regulation of the metabolome in bovine oviductal fluid depended on the stage of the oestrous cycle, the side relative to ovulation and local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected in the preovulatory, postovulatory, mid- and late luteal phases, from cyclic cows at a local abattoir (18-27 cows per stage and side). The metabolomes were assessed by proton nuclear magnetic resonance spectroscopy (H-NMR). In all, 39 metabolites were identified, among which the amino acid glycine and the energy substrates lactate and myoinositol were the most abundant at all stages...
January 5, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29288822/copper-containing-mesoporous-bioactive-glass-promotes-angiogenesis-in-an-in-vivo-zebrafish-model
#3
Lilian B Romero-Sánchez, Manuel Marí-Beffa, Paloma Carrillo, Miguel Ángel Medina, Aránzazu Díaz-Cuenca
The osteogenic and angiogenic responses of organisms to the ionic products of degradation of bioactive glasses (BGs) are being intensively investigated. The promotion of angiogenesis by copper (Cu) has been known for more than three decades. This element can be incorporated to delivery carriers, such as BGs, and the materials used in biological assays. In this work, Cu-containing mesoporous bioactive glass (MBG) in the SiO2-CaO-P2O5 compositional system was prepared incorporating 5% mol Cu (MBG-5Cu) by replacement of the corresponding amount of Ca...
December 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29273681/sorcin-is-involved-during-embryo-implantation-via-activating-vegf-pi3k-akt-pathway-in-mice
#4
Kanchan Gupta, Vijay Kumar Sirohi, Suparna Kumari, Vinay Shukla, Murli Manohar, Pooja Popli, Anila Dwivedi
Earlier studies have demonstrated the cyclic variation and also the altered expression of sorcin in endometrium during early- to mid-secretory phase transition in women with unexplained infertility. The current study was undertaken to establish the functional role of sorcin in endometrial receptivity in mice. Results indicated that sorcin was highly expressed during the window of implantation in mice and functional blockage of sorcin caused significant reduction in number of implanted blastocyst. The receptivity markers (i...
December 22, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29240275/metabolic-stress-induces-modifications-in-the-epigenetic-program-of-preimplantation-bovine-embryos
#5
R Tremblay, I Dufort, M A Sirard
The mammalian embryo is sensitive to and adapts to its metabolic environment. The mother's metabolic health and nutrient availability, for example, can modulate the oviductal fluid composition and thus embryo development. In this project, we induced energetic stress in bovine embryos during early culture to observe the epigenetic responses associated with metabolic stress, using a treatment paradigm known to decrease blastocyst rates. Embryos were generated using oocytes from slaughtered cows, and then exposed to an elevated glucose concentration (5 mM versus 0...
December 14, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29234954/low-versus-high-volume-of-culture-medium-during-embryo-transfer-a-randomized-clinical-trial
#6
George Α Sigalos, Yannis Michalopoulos, Athanasios G Kastoras, Olga Triantafyllidou, Nikos F Vlahos
PURPOSE: The aim of this prospective randomized control trial was to evaluate if the use of two different volumes (20-25 vs 40-45 μl) of media used for embryo transfer affects the clinical outcomes in fresh in vitro fertilization (IVF) cycles. METHODS: In total, 236 patients were randomized in two groups, i.e., "low volume" group (n = 118) transferring the embryos with 20-25 μl of medium and "high volume" group (n = 118) transferring the embryos with 40-45 μl of medium...
December 12, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29234458/robust-genetic-transformation-of-sorghum-sorghum-bicolor-l-using-differentiating-embryogenic-callus-induced-from-immature-embryos
#7
Srinivas Belide, Thomas Vanhercke, James Robertson Petrie, Surinder Pal Singh
Background: Sorghum (Sorghum bicolor L.) is one of the world's most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has been widely considered as a recalcitrant major crop for transformation due to accumulation of phenolic compounds, lack of model genotypes, low regeneration frequency and loss of regeneration potential through sub-cultures. Among different explants used for genetic transformation of sorghum, immature embryos are ideal over other explants...
2017: Plant Methods
https://www.readbyqxmd.com/read/29226557/lysophosphatidic-acid-accelerates-development-of-porcine-embryos-by-activating-formation-of-the-blastocoel
#8
Min-Young Shin, Seung-Eun Lee, Yeo-Jin Son, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Culture media modifications, including the addition of various factors, are important for the in vitro production of oocytes and embryos. In this study, we investigated the effects of lysophosphatidic acid (LPA) on porcine embryo development. Porcine parthenogenetic embryos were cultured with 0, 0.1, 1, and 10 μM LPA for 7 days, or cultured in basic medium until Day 4 and then treated with LPA from Days 4 to 7. No difference in the in vitro development of embryos cultured with LPA for 7 days was observed. Conversely, rates of blastocyst and over-expanded blastocyst formation were higher in the 0...
December 11, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29219221/improved-developmental-competence-in-embryos-treated-with-lycopene-during-in-vitro-culture
#9
M M R Chowdhury, Ayman Mesalam, Imran Khan, Myeong-Don Joo, Kyeong-Lim Lee, Lianguang Xu, Fahmida Afrin, Il-Keun Kong
In vitro embryo development remains suboptimal compared to in vivo development due to the challenge from various stressors associated with in vitro culturing of oocytes. When 0.2 μM lycopene was added to oocyte in vitro maturation and embryo culture media, to assess its antioxidant effects on embryo development, we observed a significant (p<0.05) increase in cleavage and blastocyst development rates compared to the corresponding controls (84.3 ± 0.6% vs. 73.1 ± 1.9% and 41.0 ± 1.4% vs. 33.4 ± 0.7%, respectively)...
December 8, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29197293/synergistic-effect-of-cysteamine-leukemia-inhibitory-factor-and-y27632-on-goat-oocyte-maturation-and-embryo-development-in%C3%A2-vitro
#10
Liyou An, Jiao Liu, Yinyan Du, Zhihui Liu, Fenli Zhang, Yanhong Liu, Xiumei Zhu, Pingping Ling, Shiwei Chang, Yeshu Hu, Yi Li, Bowen Xu, Lan Yang, Fei Xue, Giorgio Antonio Presicce, Fuliang Du
Goat oocyte in vitro maturation is associated with a variable efficiency of embryo development after in vitro fertilization (IVF). Here, we developed a novel maturation procedure to evaluate the cellular effect of cysteamine (Cys), leukemia inhibitory factor (LIF) and Y27632 on oocyte in vitro maturation in native Chinese Yangtze river white goats. Oocytes were collected by slicing ovary tissues and matured for 24 h in vitro prior to IVF. Presumptive fertilized oocytes were cultured in embryo media for 8 days...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29170163/micrornas-tiny-molecules-with-significant-role-in-mammalian-follicular-and-oocyte-development
#11
Dawit Tesfaye, Samuel Gebremedhn, Dessie Salilew-Wondim, Tsige Hailay, Michael Hoelker, Christine Grosse-Brinkhaus, Karl Schellander
The genetic regulation of female fertility (follicular development, oocyte maturation, and early preimplantation embryo development) involves the spatio-temporal regulation of those genes that play key roles in various stages of the female reproductive axis. MicroRNAs (miRNAs), a class of small non-coding RNA, are known to regulate the expression of a large proportion of such genes. In recent decades, multiple studies have aimed to determine the roles of these non-coding RNAs in mammalian follicular development, oocyte growth, and embryo development...
November 23, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29164713/importance-of-oil-overlay-for-production-of-porcine-embryos-in-vitro
#12
REVIEW
C A Martinez, E A Martinez, M A Gil
Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques...
November 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29152322/choosing-a-culture-medium-for-scnt-and-iscnt-reconstructed-embryos-from-domestic-to-wildlife-species
#13
REVIEW
A Cordova, W A King, G F Mastromonaco
Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT)...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/29142449/single-blastocyst-transfer-the-key-to-reduce-multiple-pregnancy-rates-without-compromising-the-live-birth-rate
#14
Uma M Sundhararaj, Monali V Madne, Reeta Biliangady, Sumana Gurunath, Ambika G Swamy, Indu S T Gopal
Background: Historically, to achieve higher pregnancy rates, multiple embryos were transferred after an in-vitro fertilisation (IVF). However, this practice is being reassessed, because it leads to multiple pregnancies that is known to cause adverse maternal and fetal outcomes. Aim: To compare the pregnancy outcomes in fresh IVF or intracytoplasmic sperm injection (ICSI) cycles among women undergoing elective single blastocyst transfer (eSBT) vs. those undergoing double blastocyst transfer (DBT)...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29136144/antioxidants-improve-ivf-outcome-and-subsequent-embryo-development-in-the-mouse
#15
T Truong, D K Gardner
STUDY QUESTION: What is the effect of a combination of three antioxidants (Acetyl-L-Carnitine, N-Acetyl-L-Cysteine and α-Lipoic Acid), present in IVF medium during mouse oocyte and sperm collection, on fertilization and subsequent IVF embryo development? SUMMARY ANSWER: A combination of antioxidants resulted in faster developmental times from the 2-cell stage through to expanded blastocyst stage, accompanied by a significant increase in blastocyst cell number and a reduction of intracellular hydrogen peroxide (H2O2) levels...
December 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29099693/comparative-analysis-of-sperm-selection-methods-before-the-intra-cytoplasmic-sperm-injection-procedure
#16
O Lohinova, V Pitko, N Sinilo, Ya Cherkashyna, N Hanzha
In recent years, one of the methods of assisted reproductive technologies has become increasingly widespread, namely the ICSI procedure (ICSI - Intra Cytoplasmic Sperm Injection). This method is based on artificial introduction of the spermatozoon into the cytoplasm of the oocyte. However, the complexity of this method is that the choice of a morphologically and biochemically mature spermatozoon becomes subjective. However, not so long ago, an innovative method for selecting mature, ready-to-fertilize spermatozoa was proposed...
October 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29080869/-metabolomic-profiling-in-culture-media-of-day-5-human-embryos
#17
I M Zorina, C M Eldarov, S A Yarigina, N P Makarova, D Yu Trofimov, V Yu Smolnikova, E A Kalinina, M Yu Bobrov
The aim of this study was to determine the changes of metabolomic profiles in embryonic culture media (ECM) for the evaluation of quality and implantation potential of human embryos. ECM (n=163) were collected on day 5 before transfer or cryopreservation. Some embryos were used in preimplantation genetic screening for detection of aneuploidy karyotypes. Samples were subdivided into groups according to embryo morphological classification (by Gardner), genetic analysis and implantation data. ECM were extracted with methanol, precipitates were separated by centrifugation and metabolite production of individual embryo was analysed by LC-MS (the positive ion mode)...
October 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/29061322/exposure-to-neonicotinoid-insecticides-induces-embryotoxicity-in-mice-and-rabbits
#18
Janka Babeľová, Zuzana Šefčíková, Štefan Čikoš, Alexandra Špirková, Veronika Kovaříková, Juraj Koppel, Alexander V Makarevich, Peter Chrenek, Dušan Fabian
The potential toxicity of neonicotinoids (thiacloprid, acetamiprid, thiamethoxam and clothianidin) as well as related commercial products Calypso 480SC (thiacloprid mixture), Mospilan 20SP (acetamiprid mixture) and Agita 10WG (thiamethoxam mixture) on developmental capacities and quality of preimplantation embryos was evaluated. During in vitro tests, isolated 2-cell stage mice embryos were cultured in media with various concentrations of active compounds or commercial products until blastocyst formation. As found using stereomicroscopic examination, all neonicotinoids at highest (100μM) concentration negatively affected embryonic development (P<0...
October 20, 2017: Toxicology
https://www.readbyqxmd.com/read/29051527/micro-ribonucleic-acids-and-extracellular-vesicles-repertoire-in-the-spent-culture-media-is-altered-in-women-undergoing-in-vitro-fertilization
#19
Masood Abu-Halima, Sebastian Häusler, Christina Backes, Tobias Fehlmann, Claudia Staib, Sigrun Nestel, Irina Nazarenko, Eckart Meese, Andreas Keller
MicroRNAs (miRNAs) are class of small RNA molecules with major impact on gene regulation. We analyzed the potential of miRNAs secreted from pre-implantation embryos into the embryonic culture media as biomarkers to predict successful pregnancy. Using microarray analysis, we profiled the miRNome of the 56 spent culture media (SCM) after embryos transfer and found a total of 621 miRNAs in the SCM. On average, we detected 163 miRNAs in SCM of samples with failed pregnancies, but only 149 SCM miRNAs of embryos leading to pregnancies...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29026717/addition-of-interleukin-6-to-mouse-embryo-culture-increases-blastocyst-cell-number-and-influences-the-inner-cell-mass-to-trophectoderm-ratio
#20
Rebecca L Kelley, David K Gardner
OBJECTIVE: In vitro culture of preimplantation embryos is improved by grouping embryos together in a drop of media. Individually cultured embryos are deprived of paracrine factors; with this in mind, we investigated whether the addition of a single embryo-secreted factor, interleukin-6 (IL-6), could improve the development of individually cultured embryos. METHODS: Mouse embryos were cultured individually in 2 µL of G1/G2 media in 5% oxygen and supplemented with a range of doses of recombinant mouse or human IL-6...
September 2017: Clinical and Experimental Reproductive Medicine
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