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Embryo Culture Media

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https://www.readbyqxmd.com/read/28093041/blastocyst-development-in-a-single-medium-compared-to-sequential-media
#1
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, George K Petsas, Basil C Tarlatzis, Trifon G Lainas
The aim of the present study was to compare blastocyst formation rates after embryo culture in a single medium (Global) as compared to sequential media (ISM1/BlastAssist). In this prospective trial with sibling oocytes, 542 metaphase II (MII) oocytes from 31 women were randomly and equally divided to be fertilized and cultured to the blastocyst stage in either sequential media (ISM1/BlastAssist; n = 271 MII oocytes) or a single medium (Global; n = 271 MII oocytes). In both groups, embryos were cultured in an interrupted fashion with media changes on day 3...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28090465/does-intrauterine-injection-of-low-molecular-weight-heparin-improve-the-clinical-pregnancy-rate-in-intracytoplasmic-sperm-injection
#2
Ahmed Mohamed Kamel, Yahia El-Faissal, Mona Aboulghar, Ragaa Mansour, Gamal I Serour, Mohamed Aboulghar
OBJECTIVE: Heparin can modulate proteins, and influence processes involved in implantation and trophoblastic development. This study aimed to assess the improvement of clinical pregnancy and implantation rates after local intrauterine injection of low-molecular-weight heparin (LMWH) in patients undergoing intracytoplasmic sperm injection (ICSI). METHODS: A randomised case/control design was followed in women scheduled for ICSI. The study arm was injected with intrauterine LMWH during mock embryo transfer immediately following the ovum pickup procedure, while the control arm was given an intrauterine injection with a similar volume of tissue culture media...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28073983/a-novel-embryo-culture-media-supplement-that-improves-pregnancy-rates-in-mice
#3
A R Highet, T Bianco-Miotto, K G Pringle, A Peura, S Bent, J Zhang, M B Nottle, J G Thompson, C T Roberts
The preimplantation embryo in vivo is exposed to numerous growth factors in the female reproductive tract, which are not recapitulated in embryo culture media in vitro The IGF2 and plasminogen activator systems facilitate blastocyst development. We hypothesized that the addition of IGF2 in combination with urokinase plasminogen activator (uPA) and plasminogen could improve rates of blastocyst hatching and implantation in mice. B6BcF1 and CBAB6F2 mouse embryos were divided into one of four supplemented culture media treatment groups: (1) control (media only); (2) 12...
March 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28057937/a-simple-and-rapid-flow-cytometry-based-assay-to-identify-a-competent-embryo-prior-to-embryo-transfer
#4
Eva Pallinger, Zoltan Bognar, Jozsef Bodis, Timea Csabai, Nelli Farkas, Krisztina Godony, Akos Varnagy, Edit Buzas, Julia Szekeres-Bartho
Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination of the nucleic acid (PI+) containing extracellular vesicle (EV) count in day 5 embryo culture media. 88 women undergoing IVF were included in the study. More than 1 embryos were transferred to most patients. In 58 women, the transfer resulted in clinical pregnancy, whereas in 30 women in implantation failure...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28050958/non-invasive-prediction-of-blastocyst-implantation-ongoing-pregnancy-and-live-birth-by-mass-spectrometry-lipid-fingerprinting
#5
Edson Borges, Daniela P A F Braga, Amanda Souza Setti, Daniela A Montanni, Elaine Cristina Cabral, Marcos N Eberlin, Edson G Lo Turco, Assumpto Iaconelli
OBJECTIVE: To identify lipid markers of blastocyst implantation and ongoing pregnancy by day three culture medium mass spectrometry (MS) fingerprinting. METHODS: For this study, 33 culture media samples were harvested on day three, from 22 patients undergoing day five embryo transfers. All embryos achieved the blastocyst stage and were split into groups based on their implantation (Negative Implantation, n= 14 and Positive Implantation, n= 19). The positive implantation cycles resulted in successful ongoing pregnancies...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050953/mir-142-3p-as-a-biomarker-of-blastocyst-implantation-failure-a-pilot-study
#6
Edson Borges, Amanda Souza Setti, Daniela P A F Braga, Murilo V Geraldo, Rita de Cássia S Figueira, Assumpto Iaconelli
OBJECTIVE: This study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation. METHODS: Culture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28042416/which-stage-of-mouse-embryos-is-more-appropriate-for-vitrification
#7
Nasibeh Ghandy, Abbas Ali Karimpur Malekshah
BACKGROUND: Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, morula and blastocyst stage embryos and subsequent blast formation and hatching after thawing...
January 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28032673/dental-pulp-stem-cells-model-early-life-and-imprinted-dna-methylation-patterns
#8
Keith Dunaway, Sarita Goorha, Lauren Matelski, Nora Urraca, Pamela J Lein, Ian Korf, Lawrence T Reiter, Janine M LaSalle
Early embryonic stages of pluripotency are modeled for epigenomic studies primarily with human embryonic stem cells (ESC) or induced pluripotent stem cells (iPSCs). For analysis of DNA methylation, however, ESCs and iPSCs do not accurately reflect the DNA methylation levels found in preimplantation embryos. Whole genome bisulfite sequencing (WGBS) approaches have revealed the presence of large partially methylated domains (PMDs) covering 30-40% of the genome in oocytes, preimplantation embryos, and placenta...
December 29, 2016: Stem Cells
https://www.readbyqxmd.com/read/27976360/day-three-versus-day-two-embryo-transfer-following-in-vitro-fertilization-or-intracytoplasmic-sperm-injection
#9
REVIEW
Julie Brown, Salim Daya, Phill Matson
BACKGROUND: Embryo transfer (ET) was traditionally performed two days after oocyte retrieval; however, developments in culture media have allowed embryos to be maintained in culture for longer periods. Delaying transfer from Day two to Day three would allow for further development of the embryo and might have a positive effect on pregnancy outcomes. OBJECTIVES: To determine if there are any differences in live birth and pregnancy rates when embryo transfer is performed on day three after oocyte retrieval, compared with day two, in infertile couples undergoing treatment with in vitro fertilisation (IVF), including intracytoplasmic sperm injection (ICSI)...
December 14, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27943152/oocyte-maturation-embryo-development-and-gene-expression-following-two-different-methods-of-bovine-cumulus-oocyte-complexes-vitrification
#10
Mehdi Azari, Mojtaba Kafi, Bita Ebrahimi, Roya Fatehi, Mahboobeh Jamalzadeh
OBJECTIVE: To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification. METHODS: Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: non-vitrified COCs (control), vitrification 1 group (V1); vitrification was performed by 15% ethylene glycol (EG) and 15% DMSO in holding media (TCM-199 with 20% FCS); and vitrification 2 group (V2); vitrification was performed by 40% EG in holding media...
December 10, 2016: Veterinary Research Communications
https://www.readbyqxmd.com/read/27916207/integrating-insulin-into-single-step-culture-medium-regulates-human-embryo-development-in%C3%A2-vitro
#11
Mohamed Fawzy, Mohamed Sabry, Mohamed Nour, Mohamed Y Abdelrahman, Eman Roshdy, Yasmin Magdi, Hazem Abdelghafar
OBJECTIVE: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development. DESIGN: Comparative study. SETTING: Two private centers. PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients. INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium...
December 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27901197/in-vitro-germination-of-zygotic-embryos-of-hybrid-brs-manicor%C3%A3-e-guineensis-x-e-oleifera
#12
Keila A P Bonetti, Marguerite Quoirin, Regina C Quisen, Suelen C S Lima
The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form. The purpose of this study was to optimize the in vitro germination of zygotic embryos of this hybrid comparing seed lots. The viability of zygotic embryos was evaluated by the tetrazolium test (0.075%) for 4 h. The embryos were cultured on MS and Y3 culture media, with and without the addition of NaH2PO4, as well as on MS, MS1/2 and N6 medium...
2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27890905/bone-morphogenetic-protein-1-is-expressed-in-porcine-ovarian-follicles-and-promotes-oocyte-maturation-and-early-embryonic-development
#13
Xiaocan Lei, Kuiqing Cui, Xiaoyan Cai, Yanping Ren, Qingyou Liu, Deshun Shi
In the present study, we tried to determine whether bone morphogenetic protein 1 (BMP1) plays a role in ovarian follicular development and early embryo development. We systematically investigated the expression and influence of BMP1 during porcine follicle and early embryonic development. Immunohistochemistry demonstrated that the BMP1 protein is expressed in granular cells and oocytes during follicular development, from primary to pre-ovulatory follicles, including atretic follicles and the corpus luteum. The mRNA expression of BMP1 significantly increased as the porcine follicles grew...
November 25, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27865449/characterizing-nuclear-and-mitochondrial-dna-in-spent-embryo%C3%A2-culture-media-genetic-contamination-identified
#14
Elizabeth R Hammond, Brent C McGillivray, Sophie M Wicker, John C Peek, Andrew N Shelling, Peter Stone, Larry W Chamley, Lynsey M Cree
OBJECTIVE: To characterize nuclear and mitochondrial DNA (mtDNA) in spent culture media from normally developing blastocysts to determine whether it could be used for noninvasive genetic assessment. DESIGN: Prospective embryo cohort study. SETTING: Academic center and private in vitro fertilization (IVF) clinic. PATIENT(S): Seventy patients undergoing intracytoplasmic sperm injection (ICSI) and 227 blastocysts. INTERVENTION(S): Culture media assessment, artificial blastocoele fluid collapse and DNA analysis using digital polymerase chain reaction (dPCR), long-range PCR, quantitative PCR (qPCR), and DNA fingerprinting...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27862454/effect-of-testicular-tissue-lysate-on-developmental-competence-of-porcine-oocytes-matured-and-fertilized-in-vitro
#15
A K Singh, S Naskar, B Saikia, Y Vashi, S Gupta, S Banik, M K Tamuli, V Pande, D K Sarma, S K Dhara
The objective of the present study was to investigate the effect of testicular tissue lysate (TTL) on developmental competence of germinal vesicle (GV) stage porcine oocytes. Two types of TTL were prepared through repeated freeze-thaw in liquid nitrogen, one from whole testicular tissue (wTTL) and other from either of four different sections of testes, namely just beneath the tunica albuginea (TA), from the transitional area between the seminiferous cord/tubules and the mediastinum testis (TR) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin)...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27829441/characterization-of-exosomal-release-in-bovine-endometrial-intercaruncular-stromal-cells
#16
Yong Qin Koh, Hassendrini N Peiris, Kanchan Vaswani, Sarah Reed, Gregory E Rice, Carlos Salomon, Murray D Mitchell
BACKGROUND: Cell-to-cell communication between the blastocyst and endometrium is critical for implantation. In recent years, evidence has emerged from studies in humans and several other animal species that exosomes are secreted from the endometrium and trophoblast cells and may play an important role in cell-to-cell communication maternal-fetal interface during early pregnancy. Exosomes are stable extracellular lipid bilayer vesicles that encapsulate proteins, miRNAs, and mRNAs, with the ability to deliver their cargo to near and distant sites, altering cellular function(s)...
November 9, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27821560/in-vitro-fertilization-research-is-translational-research
#17
Alan H DeCherney, Rebecca L Barnett
In vitro fertilization (IVF) is the perfect example of translational research. Changes in IVF and the IVF laboratory have been transmitted to clinical care, showing dramatic improvements in health outcomes, including notable increases in the cumulative pregnancy rate. Current research in the laboratory focusing on culture media, embryo selection criteria, and implementation of genetic testing and manipulation promises to translate to further improvements in our ability to assist human reproduction. The field of IVF and ART remains a large source for clinical and scientific discovery and development, and will require the proper interested and invested personnel, occupational structuring, and funding for continued success...
December 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27807889/viability-and-growth-of-feline-preantral-follicles-in-vitro-cultured-with-insulin-growth-factor-and-epidermal-growth-factor-supplemented-medium
#18
A E Alves, L C Padilha-Nakaghi, E A Pires-Butler, M Apparicio, Nam Silva, T F Motheo, Wrr Vicente, G C Luvoni
In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior results of follicular development, antrum formation and proliferation of granulosa cells. However, there are only few reports regarding the use of these factors on feline preantral follicle in vitro culture. Thus, the aim of this study was to investigate the effect of a combination of IGF-1 and EGF on in vitro viability and growth of preantral follicles and enclosed oocytes collected from domestic cats...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27801844/screening-auxin-response-in-vitro-culture-aptitude-and-susceptibility-to-agrobacterium-mediated-transformation-of-italian-commercial-durum-wheat-varieties
#19
Wilma Sabetta, Cristina Crosatti, Alexandra Soltész, Valentina Di Rienzo, Cinzia Montemurro
The development of a robust Agrobacterium-mediated transformation protocol for a recalcitrant species like durum wheat requires the identification and optimization of factors affecting T-DNA delivery and plant regeneration. The purpose of this research was to compare the behavior of diverse durum wheat genotypes during in vitro culture and Agrobacterium tumefaciens-mediated transformation, using immature embryos as explants. Apart from plant genotype, two of the main influencing factors for a successful genetic transformation have been examined here, i...
October 28, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27796003/long-cut-straw-provides-stable-the-rates-of-survival-pregnancy-and-live-birth-for-vitrification-of-human-blasotcysts
#20
Jung-Woo Lee, Jeong-Ho Cha, Sun-Hee Shin, Yun-Jeong Kim, Seul-Ki Lee, Hye-Jin Cha, Ji-Hae Kim, Ji-Hyun Ahn, Hye-Young Kim, Kyung-Ah Pak, Ji-Sung Yoon, Seo-Young Park, Choon-Keun Park
Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ≥38 years old, poor responder, surgical retrieval sperm, and severe male factor)...
September 2016: Balsaeng'gwa Saengsig
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