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Intracytoplasmic Sperm Injection

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https://www.readbyqxmd.com/read/28332864/effect-of-density-gradient-centrifugation-on-reactive-oxygen-species-in-human-semen
#1
Teppei Takeshima, Yasushi Yumura, Shinnosuke Kuroda, Takashi Kawahara, Hiroji Uemura, Akira Iwasaki
Density gradient centrifugation can separate motile sperm from immotile sperm and other cells for assisted reproduction, but may also remove antioxidants from seminal plasma, resulting in oxidative stress. Therefore, we investigated reactive oxygen species (ROS) concentrations and distribution in semen before and after density gradient centrifugation. We assessed semen volume, sperm concentration, sperm motility, and ROS levels before and after density gradient centrifugation (300 x g for 20 minutes) in 143 semen samples from 118 patients...
March 23, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28331619/processing-and-selection-of-surgically-retrieved-sperm-for-icsi-a-review
#2
Greta Verheyen, Biljana Popovic-Todorovic, Herman Tournaye
Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PESA) are being performed, the choice is mainly based on the cause of azoospermia (obstructive versus non-obstructive) and the surgeon's skills...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28325095/concomitant-use-of-fsh-and-low-dose-recombinant-hcg-during-the-late-follicular-phase-versus-conventional-controlled-ovarian-stimulation-for-intracytoplasmic-sperm-injection-cycles
#3
Carla Andrade Rebello Iaconelli, Amanda Souza Setti, Daniela Paes Almeida Ferreira Braga, Luiz Guilherme Louzada Maldonado, Assumpto Iaconelli, Edson Borges, Tsutomu Aoki
The objective of this study was to investigate the effects of low-dose hCG supplementation on ICSI outcomes and controlled ovarian stimulation (COS) cost. Three hundred and thirty patients undergoing ICSI were split into groups according to the COS protocol: (i) control group (n = 178), including patients undergoing conventional COS treatment; and (ii) low-dose hCG group (n = 152), including patients undergoing COS with low-dose hCG supplementation. Lower mean total doses of FSH administered and higher mean oestradiol level and mature oocyte rates were observed in the low-dose hCG group...
March 22, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28322098/effect-of-sperm-dna-fragmentation-on-clinical-outcomes-for-chinese-couples-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection
#4
Lin-Tao Xue, Rui-Xue Wang, Bing He, Wei-Ying Mo, Li Huang, Shi-Kai Wang, Xian-Bao Mao, Jun-Ping Cheng, Yue-Yue Huang, RuiZhi Liu
Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28319264/a-retrospective-study-of-the-effect-of-increasing-age-on-success-rates-of-assisted-reproductive-technology
#5
Yvonne M O'Brien, Michael Ryan, Fiona Martyn, Mary B Wingfield
OBJECTIVE: To demonstrate the effect of increasing age on the outcome of assisted reproductive technology, particularly among women aged 40 years or older. METHODS: A retrospective analysis was conducted using prospectively collected data for all in vitro fertilization and intracytoplasmic sperm injection cycles among women aged 30-35 years or 40-44 years conducted at Merrion Fertility Clinic, Dublin, Ireland, between January 1, 2010, and December 31, 2014. The relationship between age and treatment outcome was assessed...
March 20, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28299521/dynamic-of-contribution-of-ubpy-sorted-cargo-to-acrosome-biogenesis-effects-of-its-derailment-in-a-mouse-model-of-globozoospermia-the-infertile-vps54-l967q-mutant
#6
Mariarosa Gioria, Maria Enrica Pasini, Giovanna Berruti
The sperm acrosome is a specialized vacuole, a member of the family of cell-specific lysosome-related organelles. Its exocytosis, the acrosome reaction, is a crucial event during fertilization. The released acrosomal contents promote sperm penetration through the investments of the oocyte, whereas the membranous components of the acrosome are involved in sperm-oocyte interaction/fusion and oocyte activation. The way that these functionally distinct acrosomal costituents reach the vacuole during its biogenesis remains poorly understood...
March 15, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28297560/an-investigation-of-the-potential-effect-of-sperm-nuclear-vacuoles-in-human-spermatozoa-on-dna-fragmentation-using-a-neutral-and-alkaline-comet-assay
#7
E Pastuszek, J Kiewisz, P Skowronska, J Liss, M Lukaszuk, A Bruszczynska, G Jakiel, K Lukaszuk
Presence of vacuoles and degree of sperm DNA damage are considered to be the basic factors used for the assessment of sperm fertilization capacity. We aimed to investigate the link between these two parameters. According to our knowledge, this is the first study where the Comet assay was used to assess the degree of DNA fragmentation of sperm categorized by Motile Sperm Organelle Morphology Examination (MSOME) Grades. Semen samples from 10 patients were assessed. Spermatozoa were graded into four MSOME groups according to the Vanderzwalmen's criteria...
March 2017: Andrology
https://www.readbyqxmd.com/read/28292621/serum-reproductive-hormone-levels-and-ultrasound-findings-in-female-offspring-after-intracytoplasmic-sperm-injection-first-results
#8
Florence Belva, Mathieu Roelants, Veerle Vloeberghs, Johan Schiettecatte, Jan Evenepoel, Maryse Bonduelle, Michel de Vos
OBJECTIVE: To compare reproductive hormone levels and antral follicle count in intracytoplasmic sperm injection (ICSI)-conceived offspring and peers born after spontaneous conception. DESIGN: Single-center study of the reproductive health at the age of 18-22 years in the worldwide oldest cohort of female ICSI offspring. SETTING: University hospital. PATIENT(S): A longitudinally followed cohort of singleton women (n = 71) conceived by means of ICSI because of male infertility and a cross-sectionally recruited group of spontaneously conceived women of the same age (n = 81)...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292610/long-awaited-long-term-follow-up-of-reproductive-parameters-in-female-offspring-conceived-with-the-use-of-intracytoplasmic-sperm-injection
#9
EDITORIAL
Jason M Franasiak
No abstract text is available yet for this article.
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28285567/increased-scf-in-follicular-fluid-and-granulosa-cells-positively-correlates-with-oocyte-maturation-fertilization-and-embryo-quality-in-humans
#10
Jun Tan, Yang Zou, Xing-Wu Wu, Li-Feng Tian, Qiong Su, Jin-Xia He, Zhi-Hui Huang, Yan Zhao, Li-Ping Wu, Qiong-Fang Wu
Stem cell factor (SCF), which is derived from granulosa cells (GCs), plays a key role in the process of follicular development and oocyte maturation. The present study aimed to explore whether the levels of SCF in follicular fluid (FF) and GCs can be used as a potential marker for predicting oocyte developmental potential. Follicular fluid and GC samples from 150 female patients undergoing intracytoplasmic sperm injection were collected in this study. The SCF concentrations in FFs and SCF messenger RNA (mRNA) in GCs were evaluated by using enzyme-linked immunosorbent assay and real-time polymerase chain reaction, respectively...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28277744/a-modified-vitrification-method-reduces-spindle-and-chromosome-abnormalities
#11
Minghui Liu, Wenhui Zhou, Dapeng Chu, Lei Fu, Wei Sha, Shan Liu, Yuan Li
Development of an effective system for oocyte-cryopreservation is of clinical relevance in reproductive medicine. However, oocyte-preservation is not as effective as embryo preservation. In this study, we used a 37°C pre-equilibrium temperature as part of a modified vitrification method for human oocyte cryopreservation. The effect of the new method on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated in in vitro-matured human oocytes. A total of 101 in vitro-matured oocytes were randomly assigned for vitrification at pre-equilibrium temperature of 37°C (37°C Group, n=50) or at room temperature (RT Group, 22-24°C, n=51)...
March 9, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28276189/follicular-flushing-time-to-look-elsewhere-to-improve-ivf-outcomes
#12
Jason M Franasiak
Poor responders represent one of the greatest challenges in assisted reproduction. The very diagnosis threatens the proposed benefit of in vitro fertilization (IVF): attaining a numeric advantage. Thus, it may seem somewhat logical that steps to maximize this numeric advantage may prove beneficial in this population. In this issue of BJOG, Haydardedeoglu et al. assess the benefit of follicular flushing in poor responders undergoing IVF and intracytoplasmic sperm injection (IVF/ICSI) as compared to direct aspiration in a randomized control trial (Haydardedeoglu et al...
March 9, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28261628/partial-and-complete-microdeletions-of-y-chromosome-in-infertile-males-from-south-of-iran
#13
Raheleh Masoudi, Liusa Mazaheri-Asadi, Shahryar Khorasani
Y chromosome microdeletions are the second genetic cause of male infertility. The incidence of Y chromosome microdeletions can vary considerably depending on several factors, including patient selection criteria, population composition, and diagnostic protocols. They are associated with spermatogenic failure and lead to azoospermia or oligozoospermia. The advance in assisted reproductive technology and intracytoplasmic sperm injection, and the possibility of genetic defect transmission to the next generation make it necessary to improve our knowledge about the various factors leading to spermatogenic impairment...
December 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28258586/pre-implantation-genetic-screening-among-women-experiencing-recurrent-failure-of-in-vitro-fertilization
#14
Safak Hatirnaz, Alev Ozer, Ebru Hatirnaz, Melahat Atasever, Serdar Başaranoglu, Mine Kanat-Pektas, Murat Api
OBJECTIVE: To determine the efficiency of pre-implantation genetic screening (PGS) among women scheduled to undergo intracytoplasmic sperm injection who had experienced recurrent in vitro fertilization (IVF) failure. METHODS: The present retrospective cohort study reviewed the medical records of consecutive women who had experienced recurrent IVF failure and had presented at a private IVF facility in Trabzon Province, Turkey, to undergo intracytoplasmic sperm injection between May 1, 2012, and December 31, 2014...
March 3, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28257868/sperm-pretreatment-with-heparin-and-l-glutathione-sex-sorting-and-double-cryopreservation-to-improve-intracytoplasmic-sperm-injection-in-bovine
#15
Natalia Gabriela Canel, Romina Jimena Bevacqua, María Inés Hiriart, Natana Chaves Rabelo, Luiz Sergio de Almeida Camargo, Marina Romanato, Lucrecia Piñeiro de Calvo, Daniel Felipe Salamone
In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI...
April 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28254221/progesterone-estradiol-ratio-0-25-on-the-day-of-human-chorionic-gonadotropin-administration-is-associated-with-adverse-pregnancy-outcomes-in-prolonged-protocols-for-in%C3%A2-vitro-fertilization-intracytoplasmic-sperm-injection
#16
Yuan Li, Keli Luo, Yi Tang, Ge Lin, Guangxiu Lu, Fei Gong
OBJECTIVE: It has been suggested that a progesterone/estradiol ratio (P/E2) ≥ 1.0 on the day of human chorionic gonadotropin (hCG) administration indicates premature luteinization and might be associated with an adverse pregnancy; however, a lower limit of this ratio has not been determined. We aimed to identify a lower limit of P/E2 that correlates significantly with an increase in adverse pregnancies in patients undergoing a prolonged in vitro fertilization/intracytoplasmic sperm injection therapy...
February 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28251558/cryopreservation-of-very-low-numbers-of-spermatozoa-from-male-patients-undergoing-infertility-treatment-using-agarose-capsules
#17
Shota Hatakeyama, Susumu Tokuoka, Hiroyuki Abe, Yasuyuki Araki, Yasuhisa Araki
This study tried to cryopreserve low numbers of spermatozoa from men undergoing infertility treatments by inserting into agarose capsules. The capsules were transferred into a drop of cryoprotectant solution and injected 3-4 motile spermatozoa that were selected by the swim-up method by conventional intracytoplasmic sperm injection. These capsules were put on a Cryotop(®) and frozen in liquid nitrogen vapor, and then submerged into liquid nitrogen and subsequently thawed and recovered. The motile spermatozoa in the capsules were counted...
March 1, 2017: Human Cell
https://www.readbyqxmd.com/read/28247100/relationship-between-pre-icsi-meiotic-spindle-angle-ovarian-reserve-gonadotropin-stimulation-and-pregnancy-outcomes
#18
Alina M Mahfoudh, Jeong H Moon, Sara Henderson, Elena Garcia-Cerrudo, Weon-Young Son, Michael H Dahan
PURPOSE: The goals of this study are to analyze the clinical pregnancy rate as a function of the pre-intracytoplasmic sperm injection (ICSI) oocyte spindle angle and determine factors which can be associated with different spindle angles, if clinically relevant. METHODS: Fifty-eight patients, who underwent their first ICSI cycle from January to December 2013, were included. Eight hundred thirty oocytes were collected, and 648 were metaphase II (MII) on retrieval day...
February 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28228320/embryo-multinucleation-at-the-two-cell-stage-is-an-independent-predictor-of-intracytoplasmic-sperm-injection-outcomes
#19
Laurent Desch, Céline Bruno, Maxime Luu, Julie Barberet, Cécile Choux, Marjorie Lamotte, Emeline Schmutz, Paul Sagot, Patricia Fauque
OBJECTIVE: To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN: Retrospective study. SETTING: Hospital. PATIENT(S): Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28222446/the-timing-of-embryo-transfer-catheter-removal-should-it-be-delayed-or-done-immediately-a-prospective-randomized-trial
#20
Belgin Devranoğlu, Özkan Özdamar, Yiğit Çakıroğlu, Mehmet Küçükbaş, Meryem K Eken, Emek Doğer
BACKGROUND/AIMS: Data on the timing of catheter removal technique following embryo transfer (ET) are quite limited. We aimed to compare the reproductive outcomes of intracytoplasmic sperm injection (ICSI)/ET cycles in which the transfer catheter was removed immediately with those in which the catheter was removed after a delay period and hereby to evaluate the impact that the time interval before removal of the catheter following embryo deposit may have on the fertility outcomes. METHODS: A prospective randomized study was designed...
February 22, 2017: Gynecologic and Obstetric Investigation
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