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Intracytoplasmic Sperm Injection

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https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#1
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929253/positive-rheotaxis-extended-drop-a-one-step-procedure-to-select-and-recover-sperm-with-mature-chromatin-for-intracytoplasmic-sperm-injection
#2
Hamilton De Martin, Marcello S Cocuzza, Bruno C Tiseo, Guilherme J A Wood, Eduardo P Miranda, Pedro A A Monteleone, José Maria Soares, Paulo C Serafini, Miguel Srougi, Edmund C Baracat
PURPOSE: The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method. METHODS: This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS)...
September 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28928993/what-drives-embryo-development-chromosomal-normality-or-mitochondria
#3
A Bayram, I Elkhatib, A Arnanz, A Linan, F Ruiz, B Lawrenz, H M Fatemi
OBJECTIVE: To report the arrest of euploid embryos with high mtDNA content. DESIGN: A report of 2 cases. SETTING: Private fertility clinic. PATIENTS: 2 patients, 45 and 40 years old undergoing IVF treatment. INTERVENTIONS: Mature oocytes were collected and vitrified from two ovarian stimulations. Postthaw, survived mature oocytes underwent fertilization by intracytoplasmic sperm injection (ICSI)...
2017: Case Reports in Genetics
https://www.readbyqxmd.com/read/28913145/subclinical-hypothyroidism-is-it-important-in-intracytoplasmic-sperm-injection-cycles
#4
Eray Çalışkan, Rahime Nida Ergin, Deniz Can Öztekin, Bülent Kars, Seda Çakır, Kenan Sofuoğlu
OBJECTIVE: To compare intracytoplasmic sperm injection (ICSI) outcomes of women with subclinical hypothyroidism with those of euthyroid women. MATERIALS AND METHODS: A retrospective case-control study was conducted. Out of 2529 ICSI cycles evaluated, 41 women with hypothyroidism, 28 women with hyperthyroidism, and 128 women with subclinical hyperthyroidism were excluded, and 2336 cycles were analyzed. Women were identified as having subclinical hypothyroidism (case group, n=105) in the presence of a thyroid-stimulating hormone level >4...
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28913144/effect-of-pigtail-catheter-application-on-obstetric-outcomes-in-in-vitro-fertilization-intracytoplasmic-sperm-injection-pregnancies-following-hyperstimulation-syndrome
#5
Pınar Çağlar Aytaç, Hakan Kalaycı, Selçuk Yetkinel, Didem Alkaş, Seda Yüksel Şimşek, Bülent Haydardedeoğlu, Esra Bulgan Kılıçdağ
OBJECTIVE: To evaluate the effects of percutaneous pigtail catheter drainage on the outcomes of intracytoplasmic sperm injection (ICSI) pregnancies following moderate or severe ovarian hyperstimulation syndrome (OHSS). MATERIALS AND METHODS: This retrospective study included 189 patients hospitalized for OHSS following ICSI treatment in a tertiary in vitro fertilization unit between 2006 and 2014. Pigtail catheters were applied in 63 patients; the other 126 patients did not need that treatment...
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28913142/day-3-embryo-transfer-versus-day-5-blastocyst-transfers-a-prospective-randomized-controlled-trial
#6
Şafak Hatırnaz, Mine Kanat Pektaş
OBJECTIVE: This study aimed to show whether transferring day 5 embryos resulted in higher implantation and pregnancy rates than transferring day 3 embryos in Turkish women undergoing an intracytoplasmic sperm injection (ICSI) cycle. MATERIALS AND METHODS: A total of 190 women who had ICSI after retrieval of more than four oocytes on the day of fertilization check were randomly assigned to undergo embryo transfer either on day 3 or day 5. RESULTS: Day 3 and day 5 transfers were statistically similar with respect to the age of woman (p=0...
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28913141/outcome-of-intracytoplasmic-sperm-injection-after-preinstillation-of-a-gonadotropin-releasing-hormone-agonist-in-the-uterine-cavity-just-before-embryo-transfer
#7
Pınar Çağlar Aytaç, Bülent Haydardedeoğlu, Halis Özdemir, Esra Bulgan Kılıçdağ
OBJECTIVE: To evaluate the effects of a gonadotropin releasing hormone agonist (GnRHa) injection prior to embryo transfer on implantation and pregnancy rate. MATERIALS AND METHODS: We performed a retrospective analysis of patients undergoing in vitro fertilization (IVF) therapy with and without GnRHa preinstallation into the uterine cavity just before embryo transfer between January 2012 and March 2013 in a single IVF center of a university hospital. Patients were evaluated based upon implantation, pregnancy, live birth, and miscarriage rates...
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28913136/clinical-analyses-of-successful-and-previously-failed-intracytoplasmic-sperm-injection-cycle-parameters-in-patients-with-poor-ovarian-reserve
#8
Tayfun Kutlu, Enis Özkaya, Pınar Kumru, Habibe Ayvacı, Belgin Devranoğlu, İlhan Sanverdi, Yavuz Şahin, Beyhan Sağlam, Ateş Karateke
OBJECTIVE: To determine some major characteristic differences between two consecutive successful and unsuccessful intracytoplasmic sperm injection (ICSI) cycles in poor responders. MATERIALS AND METHODS: Sixty women with poor ovarian response as determined using the Bologna criteria underwent ICSI cycles following an unsuccessful trial. Some parameters of both cycles including age, body mass index (BMI), serum follicle-stimulating hormone (FSH) and estradiol levels, antral follicle count, gonadotropin dosage, duration of stimulation, antagonist starting day, duration of antagonist administration, endometrial thickness at trigger day, number of total and fertilized oocytes, embryo transfer day, number of embryo cells, and fertilization rate were compared in the same patients to identify predictors of cycles with clinical pregnancy...
March 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28906358/efficacy-evaluation-of-low-dose-aspirin-in-ivf-icsi-patients-evidence-from-13-rcts-a-systematic-review-and-meta-analysis
#9
Liping Wang, Xiaman Huang, Xueli Li, Fang Lv, Xiao He, Yu Pan, Li Wang, Xiaomei Zhang
BACKGROUND: We conducted a systematic review and meta-analysis of existing literature to evaluate the different outcomes of low-dose aspirin on patients undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI), including clinical pregnancy rate, implantation rate, live birth rate, miscarriage rate, fertilization rate, number of oocytes retrieved, and so forth. METHODS: Electronic databases including PubMed, MEDLINE, and Embase were searched between 1997 and March 2016 to identity eligible studies...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28905765/re-behavioral-cognitive-and-motor-performance-and-physical-development-of-five-year-old-children-who-were-born-after-intracytoplasmic-sperm-injection-with-the-use-of-testicular-sperm
#10
https://www.readbyqxmd.com/read/28904505/embryo-cryopreservation-following-in-vitro-maturation-for-fertility-preservation-in-a-woman-with-mullerian-adenosarcoma-a-case-report
#11
Mehdi Mohsenzadeh, Mohammad A Khalili, Nasim Tabibnejad, Nahid Yari, Azam Agha-Rahimi, Mojgan Karimi-Zarchi
In-vitro maturation (IVM) of the immature oocytes recovered from the surgically removed ovarian tissue has been considered as a process for fertility preservation in patients with cancer. Fertility preservation for a woman with Mullerian adenocarcinoma. A 37-year-old woman with Mullerian adenocarcinoma was a candidate for ovarian resection. The immature oocytes were retrieved after ovarian resection from a 37-year-old woman with Mullerian adenocarcinoma. The oocytes underwent IVM and were fertilized using intracytoplasmic sperm injection (ICSI)...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28904494/vitamin-d-deficiency-does-not-influence-reproductive-outcomes-of-ivf-icsi-a-study-of-oocyte-donors-and-recipients
#12
Manish Banker, Dipesh Sorathiya, Sandeep Shah
BACKGROUND: Vitamin D and its active metabolite, 1,25-dihydroxy vitamin D (1,25-(OH)2D3), play a significant role in reproduction. AIM: To assess the effect of serum 25-hydroxy vitamin D level on oocyte quality and endometrial receptivity by studying oocyte donors and their recipients. MATERIALS AND METHODS: This prospective study consisted of two groups: Group A (recipient group) and Group B (donor group). All the participants of Groups A1 and B1 as well as Groups A2 and B2 were subcategorized into vitamin D-deficient (<20 ng/mL) and vitamin D replete-insufficient (20 to ≥30 ng/mL), respectively...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28900814/sub-fertile-sperm-cells-exemplify-telomere-dysfunction
#13
Tal Biron-Shental, Amir Wiser, Anat Hershko-Klement, Ofer Markovitch, Aliza Amiel, Arie Berkovitch
PURPOSE: The purpose of this study was to evaluate telomere homeostasis in sub-fertile compared to fertile human sperm. METHODS: This observational, comparative study included 16 sub-fertile men who required intracytoplasmic sperm injection and 10 fertile men. At least 100 sperm cells from each participant were assessed. Main outcome measures were telomere length and telomere aggregates. Telomerase RNA component (TERC) copy number and telomere capture were assessed using fluorescence in situ hybridization technique and human telomerase reverse transcriptase (hTERT) using immunohistochemistry...
September 13, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28890427/blastocyst-transfer-does-not-improve-cycle-outcome-as-compared-to-d3-transfer-in-antagonist-cycles-with-an-elevated-progesterone-level-on-the-day-of-hcg
#14
Cem Demirel, Serkan Aydoğdu, Arzu İlknur Özdemir, Gülşah Keskin, Ercan Baştu, Faruk Buyru
OBJECTIVE: To evaluate the association between progesterone elevation on the day of human chorionic gonadotropin (hCG) administration and clinical pregnancy rates of gonadotropin-releasing hormone (GnRH) antagonist in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles with the transfer of embryos at different developmental stages (day-3 versus day-5 ETs). MATERIAL AND METHODS: This is a retrospective analysis of fresh IVF/ICSI; 194 cycles out of 2676 conducted in a single center...
September 1, 2017: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/28887104/should-we-consider-day-2-and-day-3-embryo-morphology-before-day-5-transfer-when-blastocysts-reach-a-similar-good-quality
#15
Charlene Herbemont, Solmaz Sarandi, Jeremy Boujenah, Isabelle Cedrin-Durnerin, Nathalie Sermondade, Alexandre Vivot, Christophe Poncelet, Michael Grynberg, Christophe Sifer
Clinical outcomes of 291 day-5 blastocyst transfers carried out between January 2012 and March 2016 were retrospectively compared according to their quality at day 2 and 3. Inclusion criteria were female age younger than 37 years; first or second IVF and intracytoplasmic sperm injection cycle; quality of the transferred blastocyst: blastocoele B3 or higher; inner-cell-mass A/B; trophectoderm A/B; and known implantation outcome for each transferred blastocyst. Blastocysts were classified into good-quality and poor-quality embryo groups at day 2 and 3...
August 4, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28868841/factors-that-influence-the-occurrence-of-multiple-pregnancies-after-intracytoplasmic-injection-cycles-with-two-or-three-fresh-embryo-transfers
#16
Mahbubeh Abdollahi, Reza Omani Samani, Mandana Hemat, Arezoo Arabipoor, Fatemeh Shabani, Farzad Eskandari, Masoud Salehi
Background: Multiple pregnancies are an important complication of assisted reproductive technology (ART). The present study aims to indentify the risk factors for multiple pregnancies independent of the number of transferred embryos. MATERIALS AND METHODS: This retrospective study reviewed the medical records of patients who underwent intracytoplasmic sperm injection (ICSI) cycles in Royan Institute between October 2011 and January 2012. We entered 12 factors that affected the number of gestational sacs into the poisson regression (PR) model...
October 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28868840/predicting-implantation-outcome-of-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-using-data-mining-techniques
#17
Pegah Hafiz, Mohtaram Nematollahi, Reza Boostani, Bahia Namavar Jahromi
Background: In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are two important subsets of the assisted reproductive techniques, used for the treatment of infertility. Predicting implantation outcome of IVF/ICSI or the chance of pregnancy is essential for infertile couples, since these treatments are complex and expensive with a low probability of conception. MATERIALS AND METHODS: In this cross-sectional study, the data of 486 patients were collected using census method...
October 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28866084/a-homozygous-cep135-mutation-is-associated-with-multiple-morphological-abnormalities-of-the-sperm-flagella-mmaf
#18
Yan-Wei Sha, Xiaohui Xu, Li-Bin Mei, Ping Li, Zhi-Ying Su, Xiao-Qin He, Lin Li
Multiple morphological abnormalities of the sperm flagella (MMAF) is a rare disease associated with primary infertility; however, ~50% of the genetic alterations associated with MMAF remain unclear. Here, we reported the case of a 30-year-old infertile male from a consanguineous family. Whole-exome sequencing identified a homozygous mutation in the CEP135 gene (c.A1364T:p.D455V), with CEP135 previously reported to play a role in centriole biogenesis and specifically central pair assembly. D455V-mutated proteins formed protein aggregates in the centrosome and the flagella, which might potentially affect the function of centriole assembly...
August 30, 2017: Gene
https://www.readbyqxmd.com/read/28865547/does-the-use-of-calcium-ionophore-during-artificial-oocyte-activation-demonstrate-an-effect-on-pregnancy-rate-a-meta-analysis
#19
Sughashini Murugesu, Srdjan Saso, Benjamin P Jones, Timothy Bracewell-Milnes, Thanos Athanasiou, Anastasia Mania, Paul Serhal, Jara Ben-Nagi
OBJECTIVE: To study the effect, if any, of calcium ionophore as a method of artificial oocyte activation (AOA) on pregnancy outcomes and fertilization rates. DESIGN: Meta-analysis of randomized controlled trials, prospective observational and retrospective trials, case reports, and a case-control trial. SETTING: University-affiliated teaching hospital. PATIENT(S): Infertile couples undergoing fertilization treatment...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28865546/reproductive-outcomes-of-testicular-versus-ejaculated-sperm-for-intracytoplasmic-sperm-injection-among-men-with-high-levels-of-dna-fragmentation-in-semen-systematic-review-and-meta-analysis
#20
Sandro C Esteves, Matheus Roque, Cara K Bradley, Nicolás Garrido
OBJECTIVE: To compare sperm DNA fragmentation (SDF) levels between testicular and ejaculated sperm and to evaluate outcomes of intracytoplasmic sperm injection (ICSI) with the use of testicular (Testi-ICSI) versus ejaculated (Ejac-ICSI) sperm in nonazoospermic men with high SDF. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Normo- and oligozoospermic men with high levels of SDF in semen subjected to Testi-ICSI or Ejac-ICSI...
September 2017: Fertility and Sterility
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