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https://www.readbyqxmd.com/read/27865622/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme
#1
REVIEW
Riccardo Manganelli, Maria Laura Gennaro
During envelope stress, critical inner-membrane functions are preserved by the phage-shock-protein (Psp) system, a stress response that emerged from work with Escherichia coli and other Gram-negative bacteria. Reciprocal regulatory interactions and multiple effector functions are well documented in these organisms. Searches for the Psp system across phyla reveals conservation of only one protein, PspA. However, examination of Firmicutes and Actinobacteria reveals that PspA orthologs associate with non-orthologous regulatory and effector proteins retaining functions similar to those in Gram-negative counterparts...
November 16, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27698088/interactions-between-the-cytoplasmic-domains-of-pspb-and-pspc-silence-the-yersinia-enterocolitica-phage-shock-protein-response
#2
Josué Flores-Kim, Andrew J Darwin
: The Phage shock protein (Psp) system is a widely conserved cell envelope stress response that is essential for the virulence of some bacteria, including Yersinia enterocolitica Recruitment of PspA by the inner membrane PspB•PspC complex characterizes the activated state of this response. The PspB•PspC complex has been proposed to be a stress-responsive switch, changing from an OFF to an ON state in response to an inducing stimulus. In the OFF state, PspA cannot access its binding site in the C-terminal cytoplasmic domain of PspC (PspC(CT)) because this site is bound to PspB...
October 3, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27242973/surface-proteins-and-pneumolysin-of-encapsulated-and-nonencapsulated-streptococcus-pneumoniae-mediate-virulence-in-a-chinchilla-model-of-otitis-media
#3
Lance E Keller, Jessica L Bradshaw, Haley Pipkins, Larry S McDaniel
Streptococcus pneumoniae infections result in a range of human diseases and are responsible for almost one million deaths annually. Pneumococcal disease is mediated in part through surface structures and an anti-phagocytic capsule. Recent studies have shown that nonencapsulated S. pneumoniae (NESp) make up a significant portion of the pneumococcal population and are able to cause disease. NESp lack some common surface proteins expressed by encapsulated pneumococci, but express surface proteins unique to NESp...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27208228/vipp1-has-a-disordered-c-terminal-tail-necessary-for-protecting-photosynthetic-membranes-against-stress
#4
Lingang Zhang, Hideki Kondo, Hironari Kamikubo, Mikio Kataoka, Wataru Sakamoto
Integrity of biomembranes is vital to living organisms. In bacteria, PspA is considered to act as repairing damaged membrane by forming large supercomplexes in Arabidopsis (Arabidopsis thaliana). Vulnerable to oxidative stress, photosynthetic organisms also contain a PspA ortholog called VIPP1, which has an additional C-terminal tail (Vc). In this study, Vc was shown to coincide with an intrinsically disordered region, and the role of VIPP1 in membrane protection against stress was investigated. We visualized VIPP1 by fusing it to GFP (VIPP1-GFP that fully complemented lethal vipp1 mutations), and investigated its behavior in vivo with live imaging...
July 2016: Plant Physiology
https://www.readbyqxmd.com/read/27029587/pneumococcal-surface-protein-a-plays-a-major-role-in-streptococcus-pneumoniae-induced-immunosuppression
#5
Saumyaa, Lindsey Pujanauski, Jesus Colino, Michael Flora, Raul M Torres, Elaine Tuomanen, Clifford M Snapper
Intact, inactivated Streptococcus pneumoniae [including the unencapsulated S. pneumoniae, serotype 2 strain (R36A)] markedly inhibits the humoral immune response to coimmunized heterologous proteins, a property not observed with several other intact Gram-positive or Gram-negative bacteria. In this study, we determined the nature of this immunosuppressive property. Because phosphorylcholine (PC), a major haptenic component of teichoic acid in the S. pneumoniae cell wall, and lipoteichoic acid in the S. pneumoniae membrane were previously reported to be immunosuppressive when derived from filarial parasites, we determined whether R36A lacking PC (R36A(pc-)) was inhibitory...
May 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27002134/rv2744c-is-a-pspa-ortholog-that-regulates-lipid-droplet-homeostasis-and-nonreplicating-persistence-in-mycobacterium-tuberculosis
#6
Richard M Armstrong, Katherine L Adams, Joseph E Zilisch, Daniel J Bretl, Hiromi Sato, David M Anderson, Thomas C Zahrt
UNLABELLED: Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), remains a significant cause of morbidity and mortality worldwide, despite the availability of a live attenuated vaccine and anti-TB antibiotics. The vast majority of individuals infected with M. tuberculosis develop an asymptomatic latent infection in which the bacterium survives within host-generated granulomatous lesions in a physiologically altered metabolic state of nonreplicating persistence. The granuloma represents an adverse environment, as M...
June 1, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/26370067/development-of-a-synthetic-receptor-protein-for-sensing-inflammatory-mediators-interferon-%C3%AE-and-tumor-necrosis-factor-%C3%AE
#7
T Christopher Aurand, John C March
Intestinal inflammation has been implicated in a number of diseases, including diabetes, Crohn's disease, and irritable bowel syndrome. Important components of inflammation are interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α), which are elevated both on the luminal and submucosal sides of the intestinal epithelial barrier in several diseases. Here, we developed a novel Escherichia coli based detection system for IFN-γ and TNF-α comprised of a chimeric protein and a simple signal transduction construct, which could be deployed on the luminal side of the intestine...
March 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/26330516/membrane-stored-curvature-elastic-stress-modulates-recruitment-of-maintenance-proteins-pspa-and-vipp1
#8
Christopher McDonald, Goran Jovanovic, Oscar Ces, Martin Buck
UNLABELLED: Phage shock protein A (PspA), which is responsible for maintaining inner membrane integrity under stress in enterobacteria, and vesicle-inducting protein in plastids 1 (Vipp1), which functions for membrane maintenance and thylakoid biogenesis in cyanobacteria and plants, are similar peripheral membrane-binding proteins. Their homologous N-terminal amphipathic helices are required for membrane binding; however, the membrane features recognized and required for expressing their functionalities have remained largely uncharacterized...
2015: MBio
https://www.readbyqxmd.com/read/26319873/development-of-a-novel-plasmid-free-thymidine-producer-by-reprogramming-nucleotide-metabolic-pathways
#9
Jin-Sook Kim, Min-Kyung Jeong, Bong-Seong Koo, Hyeon-Cheol Lee
A novel thymidine-producing strain of Escherichia coli was prepared by genome recombineering. Eleven genes were deleted by replacement with an expression cassette, and 7 genes were integrated into the genome. The resulting strain, E. coli HLT013, showed a high thymidine yield with a low deoxyuridine content. DNA microarrays were then used to compare the gene expression profiles of HLT013 and its isogenic parent strain. Based on microarray analysis, the pyr biosynthesis genes and 10 additional genes were selected and then expressed in HLT013 to find reasonable candidates for enhancing thymidine yield...
November 2015: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/26119301/pseudomonas-fluorescens-identification-of-fur-regulated-proteins-and-evaluation-of-their-contribution-to-pathogenesis
#10
RANDOMIZED CONTROLLED TRIAL
Li Liu, Heng Chi, Li Sun
Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories...
June 29, 2015: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/26046345/antimicrobial-activity-of-novel-synthetic-peptides-derived-from-indolicidin-and-ranalexin-against-streptococcus-pneumoniae
#11
Hassan Mahmood Jindal, Cheng Foh Le, Mohd Yasim Mohd Yusof, Rukumani Devi Velayuthan, Vannajan Sanghiran Lee, Sharifuddin Md Zain, Diyana Mohd Isa, Shamala Devi Sekaran
Antimicrobial peptides (AMPs) represent promising alternatives to conventional antibiotics in order to defeat multidrug-resistant bacteria such as Streptococcus pneumoniae. In this study, thirteen antimicrobial peptides were designed based on two natural peptides indolicidin and ranalexin. Our results revealed that four hybrid peptides RN7-IN10, RN7-IN9, RN7-IN8, and RN7-IN6 possess potent antibacterial activity against 30 pneumococcal clinical isolates (MIC 7.81-15.62µg/ml). These four hybrid peptides also showed broad spectrum antibacterial activity (7...
2015: PloS One
https://www.readbyqxmd.com/read/25899163/the-psp-system-of-mycobacterium-tuberculosis-integrates-envelope-stress-sensing-and-envelope-preserving-functions
#12
Pratik Datta, Janani Ravi, Valentina Guerrini, Rinki Chauhan, Matthew B Neiditch, Scarlet S Shell, Sarah M Fortune, Baris Hancioglu, Oleg A Igoshin, Maria Laura Gennaro
The bacterial envelope integrates essential stress-sensing and adaptive functions; thus, envelope-preserving functions are important for survival. In Gram-negative bacteria, envelope integrity during stress is maintained by the multi-gene Psp response. Mycobacterium tuberculosis was thought to lack the Psp system since it encodes only pspA and no other psp ortholog. Intriguingly, pspA maps downstream from clgR, which encodes a transcription factor regulated by the MprAB-σ(E) envelope-stress-signaling system...
August 2015: Molecular Microbiology
https://www.readbyqxmd.com/read/25118250/anionic-lipids-and-the-cytoskeletal-proteins-mreb-and-rodz-define-the-spatio-temporal-distribution-and-function-of-membrane-stress-controller-pspa-in-escherichia-coli
#13
Goran Jovanovic, Parul Mehta, Liming Ying, Martin Buck
All cell types must maintain the integrity of their membranes. The conserved bacterial membrane-associated protein PspA is a major effector acting upon extracytoplasmic stress and is implicated in protection of the inner membrane of pathogens, formation of biofilms and multi-drug-resistant persister cells. PspA and its homologues in Gram-positive bacteria and archaea protect the cell envelope whilst also supporting thylakoid biogenesis in cyanobacteria and higher plants. In enterobacteria, PspA is a dual function protein negatively regulating the Psp system in the absence of stress and acting as an effector of membrane integrity upon stress...
November 2014: Microbiology
https://www.readbyqxmd.com/read/25015773/pneumococcal-pspa-and-pspc-proteins-potential-vaccine-candidates-for-experimental-otitis-media
#14
Patricia A Schachern, Vladimir Tsuprun, Patricia Ferrieri, David E Briles, Sarah Goetz, Sebahattin Cureoglu, Michael M Paparella, Steven Juhn
OBJECTIVE: Otitis media is the most commonly diagnosed disease in ambulatory care and Streptococcuspneumoniae continues to be the most common bacterial agent. Bacterial resistance to antibiotics underscores the need for better vaccines. Current pneumococcal conjugate vaccines are modestly protective against otitis media; however, limited serotype coverage and serotype replacement have led to the investigation of pneumococcal proteins as potential vaccine candidates. Two proteins, pneumococcal surface proteins A (PspA) and C (PspC) are important virulence factors, expressed by virtually all strains...
September 2014: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/24807052/mapping-of-epitopes-recognized-by-antibodies-induced-by-immunization-of-mice-with-pspa-and-pspc
#15
Cintia F M Vadesilho, Daniela M Ferreira, Stephen B Gordon, David E Briles, Adriana T Moreno, Maria Leonor S Oliveira, Paulo L Ho, Eliane N Miyaji
Pneumococcal surface protein A (PspA) and pneumococcal surface protein C (PspC) are important candidates for an alternative vaccine against pneumococcal infections. Since these antigens show variability, the use of variants that do not afford broad protection may lead to the selection of vaccine escape bacteria. Epitopes capable of inducing antibodies with broad cross-reactivities should thus be the preferred antigens. In this work, experiments using peptide arrays show that most linear epitopes recognized by antibodies induced in mice against different PspAs were located at the initial 44 amino acids of the mature protein and that antibodies against these linear epitopes did not confer protection against a lethal challenge...
July 2014: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/24361331/the-n-terminal-amphipathic-helices-determine-regulatory-and-effector-functions-of-phage-shock-protein-a-pspa-in-escherichia-coli
#16
Goran Jovanovic, Parul Mehta, Christopher McDonald, Anthony C Davidson, Povilas Uzdavinys, Liming Ying, Martin Buck
The phage shock protein (Psp) systems found in bacteria, archaea and higher plants respond to extracytoplasmic stresses that damage the cytoplasmic membrane and enable cells to repair their membranes. The conserved membrane-associated effector protein PspA has four α-helical domains (HD1-HD4) and helps to repair the membrane as a high-order oligomer. In enterobacteria, under non-stress conditions, PspA as a low-order assembly directly inhibits its cognate transcription activator PspF. Here we show that N-terminal amphipathic helices ahA and ahB in PspA HD1 are functional determinants involved in negative gene control and stress signal perception and its transduction via interactions with the PspBC membrane stress sensors and the inner membrane (IM)...
April 3, 2014: Journal of Molecular Biology
https://www.readbyqxmd.com/read/24051749/viability-and-virulence-of-pneumolysin-pneumococcal-surface-protein-a-and-pneumolysin-pneumococcal-surface-protein-a-mutants-in-the-ear
#17
COMPARATIVE STUDY
Patricia A Schachern, Vladimir Tsuprun, Sarah Goetz, Sebahattin Cureoglu, Steven K Juhn, David E Briles, Michael M Paparella, Patricia Ferrieri
IMPORTANCE: Understanding how pneumococcal proteins affect the pathology of the middle ear and inner ear is important for the development of new approaches to prevent otitis media and its complications. OBJECTIVES: To determine the viability and virulence of Streptococcus pneumoniae mutants deficient in pneumolysin (Ply-) and pneumococcal surface protein A (PspA-) in the chinchilla middle ear. DESIGN: Bullae of chinchillas were inoculated bilaterally with wild-type (Wt), Ply-, PspA-, and Ply-/PspA- strains...
September 2013: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/23926322/noncovalent-association-of-protein-and-capsular-polysaccharide-on-bacteria-sized-latex-beads-as-a-model-for-polysaccharide-specific-humoral-immunity-to-intact-gram-positive-extracellular-bacteria
#18
Jesus Colino, Leah Duke, Clifford M Snapper
Intact Streptococcus pneumoniae expressing type 14 capsular polysaccharide (PPS14) and type III S. agalactiae containing a PPS14 core capsule identical to PPS14 exhibit noncovalent associations of PPS14 and bacterial protein, in contrast to soluble covalent conjugates of these respective Ags. Both bacteria and conjugates induce murine PPS14-specific IgG responses dependent on CD4⁺ T cells. Further, secondary immunization with conjugate and S. agalactiae, although not S. pneumoniae, results in a boosted response...
September 15, 2013: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/23533636/characterization-of-protective-immune-responses-induced-by-pneumococcal-surface-protein-a-in-fusion-with-pneumolysin-derivatives
#19
Cibelly Goulart, Thais Raquel da Silva, Dunia Rodriguez, Walter Rodrigo Politano, Luciana C C Leite, Michelle Darrieux
Pneumococcal surface protein A (PspA) and Pneumolysin derivatives (Pds) are important vaccine candidates, which can confer protection in different models of pneumococcal infection. Furthermore, the combination of these two proteins was able to increase protection against pneumococcal sepsis in mice. The present study investigated the potential of hybrid proteins generated by genetic fusion of PspA fragments to Pds to increase cross-protection against fatal pneumococcal infection. Pneumolisoids were fused to the N-terminus of clade 1 or clade 2 pspA gene fragments...
2013: PloS One
https://www.readbyqxmd.com/read/23460513/nanogel-based-pspa-intranasal-vaccine-prevents-invasive-disease-and-nasal-colonization-by-streptococcus-pneumoniae
#20
Il Gyu Kong, Ayuko Sato, Yoshikazu Yuki, Tomonori Nochi, Haruko Takahashi, Shinichi Sawada, Mio Mejima, Shiho Kurokawa, Kazunari Okada, Shintaro Sato, David E Briles, Jun Kunisawa, Yusuke Inoue, Masafumi Yamamoto, Kazunari Akiyoshi, Hiroshi Kiyono
To establish a safer and more effective vaccine against pneumococcal respiratory infections, current knowledge regarding the antigens common among pneumococcal strains and improvements to the system for delivering these antigens across the mucosal barrier must be integrated. We developed a pneumococcal vaccine that combines the advantages of pneumococcal surface protein A (PspA) with a nontoxic intranasal vaccine delivery system based on a nanometer-sized hydrogel (nanogel) consisting of a cationic cholesteryl group-bearing pullulan (cCHP)...
May 2013: Infection and Immunity
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