Corinne A Lutomski, Tarick J El-Baba, Joshua D Hinkle, Idlir Liko, Jack L Bennett, Neha V Kalmankar, Andrew Dolan, Carla Kirschbaum, Kim Greis, Leonhard H Urner, Parth Kapoor, Hsin-Yung Yen, Kevin Pagel, Christopher Mullen, John E P Syka, Carol V Robinson
Membrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires stabilization in detergent micelles that are removed prior to analysis via collisional activation. There is however a practical limit to the amount of energy which can be applied, which often precludes subsequent characterization by top-down MS. To overcome this barrier, we have applied a modified Orbitrap Eclipse Tribrid mass spectrometer coupled to an infrared laser within a high-pressure linear ion trap...
September 4, 2023: Angewandte Chemie