keyword
MENU ▼
Read by QxMD icon Read
search

three dimensional morphology

keyword
https://www.readbyqxmd.com/read/28817086/non-destructive-analysis-of-the-internal-anatomical-structures-of-mosquito-specimens-using-optical-coherence-tomography
#1
Naresh Kumar Ravichandran, Ruchire Eranga Wijesinghe, Seung-Yeol Lee, Kwang Shik Choi, Mansik Jeon, Hee-Young Jung, Jeehyun Kim
The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An...
August 17, 2017: Sensors
https://www.readbyqxmd.com/read/28816413/porcine-spermatogonial-stem-cells-self-renew-effectively-in-a-three-dimensional-culture-microenvironment
#2
Ji Eun Park, Min Hee Park, Min Seong Kim, Yeo Reum Park, Jung Im Yun, Hee Tae Cheong, Minseok Kim, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee
Generally, self-renewal of spermatogonial stem cells (SSCs) is maintained in vivo in a three-dimensional (3D) microenvironment consisting of the seminiferous tubule basement membrane, indicating the importance of the 3D microenvironment for in vitro culture of SSCs. Here, we report a 3D culture microenvironment that effectively maintains porcine SSC self-renewal during culture. Porcine SSCs were cultured in an agarose-based 3D hydrogel and in 2D culture plates either with or without feeder cells. Subsequently, the effects of 3D culture on the maintenance of undifferentiated SSCs were identified by analyzing cell colony formation and morphology, AP activity, and transcriptional and translational regulation of self-renewal-related genes and the effects on proliferation by analyzing cell viability and single cell-derived colony number...
August 17, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28815110/development-of-a-framework-for-large-scale-three-dimensional-pathology-and-biomarker-imaging-and-spatial-analytics
#3
Yanhui Liang, Fusheng Wang, Pengyue Zhang, Joel H Saltz, Daniel J Brat, Jun Kong
With the rapid advancement in large-throughput scanning technologies, digital pathology has emerged as platform with promise for diagnostic approaches, but also for high-throughput quantitative data extraction and analysis for translational research. Digital pathology and biomarker images are rich sources of information on tissue architecture, cell diversity and morphology, and molecular pathway activation. However, the understanding of disease in three-dimension (3D) has been hampered by their traditional two-dimension (2D) representations on histologic slides...
2017: AMIA Summits on Translational Science Proceedings
https://www.readbyqxmd.com/read/28814861/the-influence-of-direct-laser-metal-sintering-implants-on-the-early-stages-of-osseointegration-in-diabetic-mini-pigs
#4
Naiwen Tan, Xiangwei Liu, Yanhui Cai, Sijia Zhang, Bo Jian, Yuchao Zhou, Xiaoru Xu, Shuai Ren, Hongbo Wei, Yingliang Song
BACKGROUND: High failure rates of oral implants have been reported in diabetic patients due to the disruption of osseointegration. The aim of this study was to investigate whether direct laser metal sintering (DLMS) could improve osseointegration in diabetic animal models. METHODS: Surface characterizations were carried out on two types of implants. Cell morphology and the osteogenic-related gene expression of MG63 cells were observed under conditions of DLMS and microarc oxidation (MAO)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28813431/skeleton-of-an-unusual-cat-sized-marsupial-relative-metatheria-marsupialiformes-from-the-middle-eocene-lutetian-44-43-million-years-ago-of-turkey
#5
A Murat Maga, Robin M D Beck
We describe a near-complete, three-dimensionally preserved skeleton of a metatherian (relative of modern marsupials) from the middle Eocene (Lutetian: 44-43 million years ago) Lülük member of the Uzunçarşıdere Formation, central Turkey. With an estimated body mass of 3-4 kg, about the size of a domestic cat (Felis catus) or spotted quoll (Dasyurus maculatus), it is an order of magnitude larger than the largest fossil metatherians previously known from the Cenozoic of the northern hemisphere. This new taxon is characterised by large, broad third premolars that probably represent adaptations for hard object feeding (durophagy), and its craniodental morphology suggests the capacity to generate high bite forces...
2017: PloS One
https://www.readbyqxmd.com/read/28812179/a-hybrid-co-culture-model-with-endothelial-cells-designed-for-the-hepatic-tissue-engineering
#6
Xiaoning Yang, Xin Wang, Xiaobo Huang, Ruiqiang Hang, Xiangyu Zhang, Bin Tang
The cultured hepatic cells in vitro are prone to losing their characteristic morphologies and biological functions. To avoid this problem, a hybrid co-culture system was proposed to elucidate the effect of cellular communication on the phenotype of hepatic cells. A monolayer of endothelial cells (ECs) was co-cultured on the surface of a three-dimensional (3D) scaffold embedded with HepG2 cells. In this hybrid co-culture system, the growth of encapsulated hepatic cells is barely influenced by the co-cultured ECs...
August 10, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28810054/dynamic-self-assembly-of-gold-polymer-nanocomposites-ph-encoded-switching-between-1d-nanowires-and-3d-nanosponges
#7
Da-Hui Qu, Qi Zhang, Tian-Yi Xu, Cai-Xin Zhao, Wei-Hang Jin, Qian Wang
The design of tunable dynamic self-assembly of nanoparticles with switchable assembled dimensions and morphologies is a challenging goal whose realization is vital for the evolution of smart nanomaterials. Herein we show the chitosan polymer as an effective supramolecular "glue" for aldehyde-modified Au nanoparticles to reversibly modulate the states of self-assembled nanocomposites. By simultaneous integration of dynamic covalent Schiff base interactions and noncovalent hydrogen bonds, the chitosan/Au nanocomposites could reversibly transform their assembled morphologies from one-dimensional nanowires to three-dimensional nanosponges in response to the variation of pH value...
August 15, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28808270/a-low-permeability-microfluidic-blood-brain-barrier-platform-with-direct-contact-between-perfusable-vascular-network-and-astrocytes
#8
Seokyoung Bang, Seung-Ryeol Lee, Jihoon Ko, Kyungmin Son, Dongha Tahk, Jungho Ahn, Changkyun Im, Noo Li Jeon
A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies media to the neural cells (neural channel, NC). Compared to co-cultures supplied with only one type of medium (or 1:1 mixture), best barrier properties and viability were obtained with culturing HUVECs with endothelial growth medium (EGM) and neural cells with neurobasal medium supplemented with fetal bovine serum (NBMFBS) independently...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28808216/modeling-and-validating-three-dimensional-human-normal-cervix-and-cervical-cancer-tissues-in-vitro
#9
Anna Karolina Zuk, Xuesong Wen, Stephen Dilworth, Dong Li, Lucy Ghali
The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-Din vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues. De-epidermised dermis (DED) was used as a scaffold for both models. Morphological analyses were conducted by using hematoxylin and eosin staining and characteristics of the models were studied by analyzing the expression of different structural cytokeratins and differential protein marker Mad1 using immunohistochemical technique...
January 19, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28807411/timp3-is-regulated-by-pericytes-upon-shear-stress-detection-leading-to-a-modified-endothelial-cell-response
#10
C Schrimpf, T Koppen, J S Duffield, U Böer, S David, W Ziegler, A Haverich, O E Teebken, M Wilhelmi
OBJECTIVES: Atherosclerosis is a hallmark of cardiovascular disease. Shear stress on endothelial cells has been linked to atherogenesis and to fibrous cap thinning and rupture. Pericytes reside in the sub-endothelial space of vessels and have vasoprotective effects. They are subjected to shear stress when endothelial cell integrity is disrupted. The aim was to investigate the susceptibility and response of pericytes to shear stress. METHODS: Endothelial cells and pericytes were seeded in two dimensional monocultures and co-cultures, and in a novel three dimensional co-culture system and were subjected to no, low and high shear stress (0, 10, 30 dyne/cm(2)) for 48 h...
August 11, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28802849/silk-fibroin-chitosan-scaffold-with-tunable-properties-and-low-inflammatory-response-assists-the-differentiation-of-bone-marrow-mesenchymal-stem-cells
#11
Da-Wei Li, Xiaohua Lei, Feng-Li He, Jin He, Ya-Li Liu, Ya-Jing Ye, Xudong Deng, Enkui Duan, Da-Chuan Yin
The physical and chemical properties of the scaffold are known to play important roles in three-dimensional (3D) cell culture, which always determine the cellular fate or the results of implantation. To control these properties becomes necessary for meeting the requirements of a variety of tissue engineering applications. In this study, a series of silk fibroin/chitosan (SF/CS) scaffolds with tunable properties were prepared using freeze-drying method, and the rat bone marrow-derived mesenchymal stem cells (BM-MSCs) were seeded in these scaffolds to evaluate their availability of use in tissue engineering...
August 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28801212/intraoperative-validation-of-quantitative-t2-mapping-in-patients-with-articular-cartilage-lesions-of-the-knee
#12
Stefan Soellner, Axel Goldmann, Daniel Muelheims, Goetz H Welsch, Milena Pachowsky
OBJECTIVE: The aim of this study was to compare T2 relaxation times of knee cartilage with intraoperative results for the assessment of early osteoarthritis (OA) and to define T2 values for the differentiation between healthy and degenerated cartilage. DESIGN: Twenty-one patients with cartilage lesions or moderate OA were examined using 3T MRI. In this prospective study, a total of 882 regions of interest (ROIs) were examined by a sagittal, multi-echo, spin-echo T2 sequence and a morphological high-resolution three-dimensional, fat-saturated proton-density space sequence...
August 8, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28800287/measuring-and-establishing-the-accuracy-and-reproducibility-of-3d-printed-medical-models
#13
Elizabeth George, Peter Liacouras, Frank J Rybicki, Dimitrios Mitsouras
Despite the rapid growth of three-dimensional (3D) printing applications in medicine, the accuracy and reproducibility of 3D printed medical models have not been thoroughly investigated. Although current technologies enable 3D models to be created with accuracy within the limits of clinical imaging spatial resolutions, this is not always achieved in practice. Inaccuracies are due to errors that occur during the imaging, segmentation, postprocessing, and 3D printing steps. Radiologists' understanding of the factors that influence 3D printed model accuracy and the metrics used to measure this accuracy is key in directing appropriate practices and establishing reference standards and validation procedures...
August 11, 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/28796108/research-and-clinical-application-of-three-dimensional-location-of-amygdaloid-body
#14
Haobo Duan, Pei Shang, Peng Bai, Zilu Li, Youqiong Li, Kailiang Cheng, Meiying Xin
Accurately representing the spatial location of the amygdaloid body can lay an anatomical basis for the neurosurgery operation for amputation of the amygdaloid body through lateral fissure approach. As we know, there are a number of nerve nucleuses and essential structures locating around amygdaloid body in our brain, especially optic tract. However, only few research had been done to protect these tissues or nerve nucleuses. Thus, we reconstructed the three-dimensional images of the amygdaloid body of the human brain and established a coordinate system...
August 8, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28794917/microfluidic-device-for-primary-tumor-spheroid-isolation
#15
Jiaojiao Zhou, Jimmy Su, Xiaotong Fu, Lei Zheng, Zhizhong Yin
BACKGROUND: Traditional two-dimensional (2-D) monolayer cell culture is vastly different from in vivo physiological conditions, which can lead to inaccurate or insufficient data in areas where response and efficacy within humans are being investigated, such as drug discovery, pathology studies, etc. Misleading results arise from two main disadvantages of monolayer cell culture. First, after several passages, cell lines lose many features from their original in vivo state. Second, the morphology of cells cultured in a monolayer is much different from the cell morphology in three-dimensional (3-D) in vivo conditions, thus resulting in altered cellular function...
2017: Experimental Hematology & Oncology
https://www.readbyqxmd.com/read/28794276/a-test-for-paedomorphism-in-domestic-pig-cranial-morphology
#16
Allowen Evin, Joseph Owen, Greger Larson, Mélanie Debiais-Thibaud, Thomas Cucchi, Una Strand Vidarsdottir, Keith Dobney
Domestic animals are often described as paedomorphic, meaning that they retain juvenile characteristics into adulthood. Through a three-dimensional landmark-based geometric morphometric analysis of cranial morphology at three growth stages, we demonstrate that wild boar (n = 138) and domestic pigs (n = 106) (Sus scrofa) follow distinct ontogenetic trajectories. With the exception of the size ratio between facial and neurocranial regions, paedomorphism does not appear to be the primary pattern describing the observed differences between wild and domestic pig cranial morphologies...
August 2017: Biology Letters
https://www.readbyqxmd.com/read/28794042/palmitoylation-contributes-to-membrane-curvature-in-influenza-a-virus-assembly-and-hemagglutinin-mediated-membrane-fusion
#17
Petr Chlanda, Elena Mekhedov, Hang Waters, Alexander Sodt, Cindi Schwartz, Vinod Nair, Paul S Blank, Joshua Zimmerberg
The highly conserved cytoplasmic tail of influenza virus glycoprotein hemagglutinin (HA) contains three cysteines, post-translationally modified by covalently bound fatty acids. While viral HA acylation is crucial in virus replication, its physico-chemical role is unknown. We used virus-like particles (VLP) to study the effect of acylation on morphology, protein incorporation, lipid composition, and membrane fusion. De-acylation interrupted HA-M1 interactions since de-acylated mutant HA fail to incorporate an M1 layer within spheroidal VLP, and filamentous particles incorporated increased numbers of neuraminidase (NA)...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28791464/a-computed-tomographic-anatomical-study-of-the-upper-sacrum-application-for-a-user-guide-of-pelvic-fixation-with-iliosacral-screws-in-adult-spinal-deformity
#18
Arnaud Dubory, Houssam Bouloussa, Guillaume Riouallon, Stéphane Wolff
PURPOSE: Widely used in traumatic pelvic ring fractures, the iliosacral (IS) screw technique for spino-pelvic fixation remains anecdotal in adult spinal deformity. The objective of this study was to assess anatomical variability of the adult upper sacrum and to provide a user guide of spino-pelvic fixation with IS screws in adult spinal deformity. METHODS: Anatomical variability of the upper sacrum according to age, gender, height and weight was sought on 30 consecutive pelvic CT-scans...
August 8, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28790902/integration-of-brain-and-skull-in-prenatal-mouse-models-of-apert-and-crouzon-syndromes
#19
Susan M Motch Perrine, Tim Stecko, Thomas Neuberger, Ethylin W Jabs, Timothy M Ryan, Joan T Richtsmeier
The brain and skull represent a complex arrangement of integrated anatomical structures composed of various cell and tissue types that maintain structural and functional association throughout development. Morphological integration, a concept developed in vertebrate morphology and evolutionary biology, describes the coordinated variation of functionally and developmentally related traits of organisms. Syndromic craniosynostosis is characterized by distinctive changes in skull morphology and perceptible, though less well studied, changes in brain structure and morphology...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28790477/pediatric-lumbar-pedicle-screw-placement-using-navigation-templates-a-cadaveric-study
#20
Xing Wang, Jun Shi, Shojie Zhang, Zhifeng Zhang, Xiaohe Li, Zhijun Li
BACKGROUND: Pedicle screw technique is commonly used treatment of adult spinal trauma, tumor, degeneration. The application of pedicle screws is very challenging in children because children have a fast growing spine and spinal pedicle morphology of children and adult has large difference. 3 D reconstruction individual navigation templates improve the success rate of pediatric pedicle screw system. This study is aimed to provide a precise method for lumbar spine pedicle screw placement in children using computer-aided design and rapid prototyping technology...
July 2017: Indian Journal of Orthopaedics
keyword
keyword
98048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"