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https://www.readbyqxmd.com/read/28449392/osmotic-stress-decreases-complexity-underlying-the-electrophysiological-dynamic-in-soybean
#1
G F R Saraiva, A S Ferreira, G M Souza
Research rationale: Studies on plant electrophysiology are mostly focused on specific traits of single cells. Inspired by the complexity of the signaling network in plants and by analogies with neurons in human brains, we have sought for evidences of high complexity in the electrical dynamics of plant signaling and a likely relation with environmental cues. Central methods: An EEG-like standard protocol was adopted for high-resolution measurements of the electrical signal in Glycine max seedlings. The signals were continuously recorded in the same plants before and after the osmotic stimuli with a -2 MPa mannitol solution...
April 27, 2017: Plant Biology
https://www.readbyqxmd.com/read/28447893/ultra-small-solid-archaeolipid-nanoparticles-for-active-targeting-to-macrophages-of-the-inflamed-mucosa
#2
Leticia Herminia Higa, Horacio Emanuel Jerez, Marcelo Alexandre de Farias, Rodrigo Villares Portugal, Eder Lilia Romero, Maria Jose Morilla
AIM: Develop nanoparticulate agents for oral targeted delivery of dexamethasone (Dex) to macrophages of inflamed mucosa. MATERIALS & METHODS: Solid archaeolipid nanoparticles (SAN-Dex) (compritol/Halorubrum tebenquichense polar archaeolipids/soybean phosphatidylcholine/Tween-80 4; 0.9; 0.3; 3% w/w) loaded with Dex were prepared. Their mucopenetration, stability under digestion and in vitro anti-inflammatory activity, were determined. RESULTS: Ultra-small SAN-Dex strongly reduced the levels of TNF-α, IL-6 and IL-12 on J774A1 cells stimulated with lipopolysaccharides as compared with free Dex or loaded in ordinary solid lipid nanoparticles-Dex...
April 27, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28447366/effects-of-dietary-lysine-levels-on-the-concentrations-of-selected-nutrient-metabolites-in-blood-plasma-of-late-stage-finishing-pigs
#3
N Regmi, T Wang, M A Crenshaw, B J Rude, S F Liao
Lysine is the first-limiting amino acid (AA) in typical swine diets and plays very important roles in promoting growth performance of pigs. This research was conducted to study the effects of dietary lysine on blood plasma concentrations of protein, carbohydrate, and lipid metabolites of pigs. Eighteen crossbred finishing pigs (nine barrows and nine gilts; initial BW 92.3 ± 6.9 kg) were individually penned in an environment controlled barn. Pigs were assigned to three dietary treatments according to a randomized complete block design with gender as block and pig as experimental unit (6 pigs/treatment)...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28445652/identification-of-quantitative-trait-loci-underlying-seed-protein-content-of-soybean-including-main-epistatic-and-qtl-%C3%A3-environment-effects-in-different-regions-of-northeast-china
#4
Weili Teng, Wen Li, Q I Zhang, Depeng Wu, Xue Zhao, Haiyan Li, Yingpeng Han, Wenbin Li
The objective here was to identify QTL, their epistatic effects and QTLs x environments interaction underlying soybean protein content (PC). The mapping population, consisted of 129 recombinant inbred lines (RILs), was created by crossing 'Dongnong46' and 'L-100'. Phenotypic data of the parents and RILs were collected for four years in three locations of Heilongjiang Province of China. A total of 213 simple sequence repeat markers were used to construct a genetic linkage map. Eight QTLs, located on seven chromosomes (Chr), were identified to be associated with PC among the ten tested environments...
April 26, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28444972/production-and-application-of-a-thermostable-lipase-from-serratia-marcescens-in-detergent-formulation-and-biodiesel-production
#5
Edgar Edurman García-Silvera, Fernando Martínez-Morales, Brandt Bertrand, Daniel Morales-Guzman, Nashbly Sarela Rosas-Galván, Renato León-Rodríguez, María Del Refugio Trejo-Hernández
In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2(2) factorial design the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05 % soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50°C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55°C)...
April 26, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28444878/what-do-farmers-weed-control-decisions-imply-about-glyphosate-resistance-evidence-from-surveys-of-u-s-corn-fields
#6
Seth J Wechsler, Jonathan R McFadden, David J Smith
BACKGROUND: The first case of glyphosate-resistant weeds in the United States was documented in 1998, two years after the commercialization of genetically-engineered herbicide-resistant (HR) corn and soybeans. Currently, over 15 glyphosate-resistant weed species affect U.S. crop production areas. These weeds have the potential to reduce yields, increase costs, and lower farm profitability. The objective of our study is to develop a behavioral model of farmers' weed management decisions and use it to analyze weed resistance to glyphosate in U...
April 26, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28444301/soybean-nima-related-kinase1-promotes-plant-growth-and-improves-salt-and-cold-tolerance
#7
Wen-Jia Pan, Jian-Jun Tao, Tong Cheng, Ming Shen, Jin-Biao Ma, Wan-Ke Zhang, Qin Lin, Biao Ma, Shou-Yi Chen, Jin-Song Zhang
NEK (NIMA-related kinase) is known as a family of serine/threonine kinases which mainly participate in microtubule-related mitotic events in fungi, mammals and other eukaryotes. Our previous studies found that Arabidopsis NEK6 plays an important role in plant response to abiotic stress. We further investigated roles of NEK family in soybean and found that at least eight members can respond to abiotic stresses. Among them, only GmNEK1, a novel NEK member which is distantly-related to Arabidopsis NEK6, enhanced plant growth and promoted salt and cold tolerance in transgenic Arabidopsis plants...
April 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28444249/quantitative-analysis-of-the-3d-cell-shape-changes-driving-soybean-germination
#8
Nátali Maidl Souza, Alexander T Topham, George W Bassel
Seed germination is central to plant establishment and is the starting point for the majority of world agriculture. This transition from seed to seedling has been extensively studied at an organ level, while few studies have examined the cellular events which underlie it. Reports in the model species Arabidopsis have identified a radicle-derived wave of cell expansion underlying the germination process. Whether this spatiotemporal pattern of cell expansion is specific to this model plant or conserved in other species remains unknown...
April 22, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28444217/direct-and-indirect-effects-of-pesticides-on-the-insidious-flower-bug-hemiptera-anthocoridae-under-laboratory-conditions
#9
Nathan J Herrick, Raymond A Cloyd
Greenhouse producers are interested in integrating natural enemies along with pesticides to suppress western flower thrips, Frankliniella occidentalis (Pergande), populations. The insidious flower bug, Orius insidiosus (Say), is a commercially available natural enemy of western flower thrips. We conducted a series of laboratory experiments to determine the direct and indirect effects of 28 pesticides (insecticides, miticides, and fungicides), 4 pesticide mixtures, and 4 surfactants (36 total treatments plus a water control) on the adult O...
April 21, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28443124/fine-mapping-of-a-resistance-gene-rpshn-that-controls-phytophthora-sojae-using-recombinant-inbred-lines-and-secondary-populations
#10
Jingping Niu, Na Guo, Jutao Sun, Lihong Li, Yongce Cao, Shuguang Li, Jianli Huang, Jinming Zhao, Tuanjie Zhao, Han Xing
Phytophthora root rot (PRR), caused by Phytophthora sojae, has negative effects on soybean yield in China and can be controlled by identifying germplasm resources with resistance genes. In this study, the resistance locus RpsHN in the soybean line Meng8206 was mapped using two mapping populations. Initial mapping was realized using two recombinant inbred line (RIL) populations and included 103 F6:8 RILs derived from a cross of Meng8206 × Linhedafenqing, including 2600 bin markers, and 130 F6:8 RILs derived from a cross of Meng8206 × Zhengyang148, including 2267 bin markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28443113/barcode-system-for-genetic-identification-of-soybean-glycine-max-l-merrill-cultivars-using-indel-markers-specific-to-dense-variation-blocks
#11
Hwang-Bae Sohn, Su-Jeong Kim, Tae-Young Hwang, Hyang-Mi Park, Yu-Young Lee, Kesavan Markkandan, Dongwoo Lee, Sunghoon Lee, Su-Young Hong, Yun-Ho Song, Bon-Cheol Koo, Yul-Ho Kim
For genetic identification of soybean [Glycine max (L.) Merrill] cultivars, insertions/deletions (InDel) markers have been preferred currently because they are easy to use, co-dominant and relatively abundant. Despite their biological importance, the investigation of InDels with proven quality and reproducibility has been limited. In this study, we described soybean barcode system approach based on InDel makers, each of which is specific to a dense variation block (dVB) with non-random recombination due to many variations...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28443101/characterization-of-soybean-genetically-modified-for-drought-tolerance-in-field-conditions
#12
Renata Fuganti-Pagliarini, Leonardo C Ferreira, Fabiana A Rodrigues, Hugo B C Molinari, Silvana R R Marin, Mayla D C Molinari, Juliana Marcolino-Gomes, Liliane M Mertz-Henning, José R B Farias, Maria C N de Oliveira, Norman Neumaier, Norihito Kanamori, Yasunari Fujita, Junya Mizoi, Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki, Alexandre L Nepomuceno
Drought is one of the most stressful environmental factor causing yield and economic losses in many soybean-producing regions. In the last decades, transcription factors (TFs) are being used to develop genetically modified plants more tolerant to abiotic stresses. Dehydration responsive element binding (DREB) and ABA-responsive element-binding (AREB) TFs were introduced in soybean showing improved drought tolerance, under controlled conditions. However, these results may not be representative of the way in which plants behave over the entire season in the real field situation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28441441/a-novel-multiplexed-probe-based-quantitative-pcr-assay-for-the-soybean-root-and-stem-rot-pathogen-phytophthora-sojae-utilizes-its-transposable-element
#13
James S Haudenshield, Jeong Y Song, Glen L Hartman
Phytophthora root rot of soybean [Glycine max (L.) Merr.] is caused by the oomycete Phytophthora sojae (Kaufm. & Gerd.). P. sojae has a narrow host range, consisting primarily of soybean, and it is a serious pathogen worldwide. It exists in root and stem tissues as mycelium, wherein it can form oospores which subsequently germinate to release motile, infectious zoospores. Molecular assays detecting DNA of P. sojae are useful in disease diagnostics, and for determining the presence of the organism in host tissues, soils, and runoff or ponded water from potentially infested fields...
2017: PloS One
https://www.readbyqxmd.com/read/28441038/phytoextraction-of-nitrogen-and-phosphorus-by-crops-grown-in-a-heavily-manured-dark-brown-chernozem-under-contrasting-soil-moisture-conditions
#14
Ikechukwu Agomoh, Xiying Hao, Francis Zvomuya
Repeated uptake of excess nutrients by crops in soils with a long history of manure application may be a viable option for reducing the nutrient levels. In this greenhouse study, we examined the effectiveness of six 40-day cycles of barley, canola, corn, oat, pea, soybean, and triticale, all grown under two moisture regimes (100% and 50% soil field capacity, SFC), at extracting nitrogen (N) and phosphorus (P) from a Dark Brown Chernozem that had received 180 Mg ha(-1) (wet wt.) of beef cattle feedlot manure annually for 38 years...
April 25, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28438092/characterization-by-lectin-histochemistry-of-two-subpopulations-of-parietal-cells-in-the-rat-gastric-glands
#15
Laura Gómez-Santos, Edurne Alonso, Lucio Díaz-Flores, Juan F Madrid, Francisco J Sáez
Parietal cells undergo a differentiation process while they move from the isthmus toward the pits and the base region of the gastric gland. The aim of this work was to analyze the rat gastric glands by lectin histochemistry to show the glycans expressed by upper (young) and lower (old) parietal cells. We used lectins recognizing the most frequent sugar moieties in mammals. Each lectin was assayed alone and in combination with several deglycosylation pretreatments: (1) β-elimination, which removes O-linked oligosaccharides; (2) incubation with Peptide-N-glycosidase F, to remove N-linked glycans; (3) acid hydrolysis, which removes terminal sialic acid moieties; (4) methylation-saponification, to remove sulfate groups from sugar residues; and (5) glucose oxidase, a technique carried out with the lectin concanavalin A to convert glucose into gluconic acid...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28436718/design-of-a-novel-curcumin-soybean-phosphatidylcholine-complex-based-targeted-drug-delivery-systems
#16
Jiajiang Xie, Yanxiu Li, Liang Song, Zhou Pan, Shefang Ye, Zhenqing Hou
Recently, the global trend in the field of nanomedicine has been toward the design of combination of nature active constituents and phospholipid (PC) to form a therapeutic drug-phospholipid complex. As a particular amphiphilic molecular complex, it can be a unique bridge of traditional dosage-form and novel drug delivery system. In thisarticle, on the basis of drug-phospholipid complex technique and self-assembly technique, we chose a pharmacologically safe and low toxic drug curcumin (CUR) to increase drug-loading ability, achieve controlled/sustained drug release and improve anticancer activity...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28435105/organ-specific-proteomics-of-soybean-seedlings-under-flooding-and-drought-stresses
#17
Xin Wang, Ehsaneh Khodadadi, Baratali Fakheri, Setsuko Komatsu
Organ-specific analyses enrich the understanding of plant growth and development under abiotic stresses. To elucidate the cellular responses in soybean seedlings exposed to flooding and drought stresses, organ-specific analysis was performed using a gel-free/label-free proteomic technique. Physiological analysis indicated that enzyme activities of alcohol dehydrogenase and delta-1-pyrroline-5-carboxylate synthase were markedly increased in leaf and root of plants treated with 6days of flooding and drought stresses, respectively...
April 18, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28435032/clinical-efficacy-of-1-year-intensive-systematic-dietary-manipulation-as-complementary-and-alternative-medicine-therapies-on-female-interstitial-cystitis-bladder-pain-syndrome-patients
#18
Hitoshi Oh-Oka
OBJECTIVE: To evaluate the clinical efficacy of intensive systematic dietary manipulation (ISDM) for female patients with interstitial cystitis (IC)/bladder pain syndrome (BPS) in stable condition who were followed in our hospital. MATERIALS AND METHODS: In cooperation with the nutrition control team, we created a basic IC/BPS diet menu for 1 month. Data regarding daily food intake and food-related symptoms were collected by detailed interview of each patient, we set meal menu to control IC/BPS symptoms and advised the patients to reduce the intake of specific food items to the maximum possible extent...
April 20, 2017: Urology
https://www.readbyqxmd.com/read/28434760/omega-3-fatty-acid-supplementation-is-associated-with-oxidative-stress-and-dyslipidemia-but-does-not-contribute-to-better-lipid-and-oxidative-status-on-hemodialysis-patients
#19
Andresa Marques de Mattos, José Abrão Cardeal da Costa, Alceu Afonso Jordão Júnior, Paula Garcia Chiarello
OBJECTIVE: The aim of the study was to explore the effects of n-3 polyunsaturated fatty acids (PUFA) supplementation in physiological doses on oxidative stress (OS) and dyslipidemia in patients on hemodialysis (HD). DESIGN AND METHODS: Randomized, double-blind, controlled, experimental trial. A total of 88 HD patients ≥18 years old and on HD for at least 6 months. A total of 43 patients received 1.28 g/day of n-3 PUFA, and 45 other patients received soybean oil for 12 weeks...
April 20, 2017: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/28434727/short-term-effects-of-soybean-oil-supplementation-on-performance-digestion-and-metabolism-in-dairy-cows-fed-sugarcane-based-diets
#20
João Paulo P Rodrigues, Ricardo M de Paula, Luciana N Rennó, Marta M S Fontes, Andreia F Machado, Sebastião de C Valadares Filho, Pekka Huhtanen, Marcos I Marcondes
We aimed to quantify the productive and metabolic responses, and digestive changes in dairy cows fed various concentrations of soybean oil (SBO) in high-concentrate, sugarcane-based diets. Eight rumen-cannulated multiparous Holstein cows in mid lactation (574 ± 19.1 kg of body weight and 122 ± 6.9 d in milk), averaging 22.5 ± 1.22 kg/d of milk were assigned to replicated 4 × 4 Latin squares. The experimental period lasted 21 d as follows: 14 d for adaptation, followed by a sampling period from d 15 to 21...
April 20, 2017: Journal of Dairy Science
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