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https://www.readbyqxmd.com/read/29345745/comparison-of-flow-cytometric-methods-for-the-enumeration-of-residual-leucocytes-in-leucoreduced-blood-products-a-multicenter-study
#1
Yang Zeng, Michelle Dabay, Virginia George, Shalini Seetharaman, Monika de Arruda Indig, Sharon Graminske, Nicole Kimpel, Anna Schmidt, Amanda Boerner, Sarai Paradiso, Tatyana Delman, Yunyao Li, Viktoriya Litvak, Farzad Oreizy, Angela Chen, Maryam Saleminik, Fred Mosqueda, Anna Lin, Kevin Judge
The BD FACSVia™ System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount™ kit with the BD FACSCalibur™ flow cytometer. Leucoreduced platelet (PLT, n = 252) and red blood cell (RBC, n = 278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter-site reproducibility on the BD FACSVia System at three sites over 20 days...
January 18, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29336630/ameliorating-role-of-lycopene-tomato-puree-and-spirulina-tomato-puree-on-the-hematology-of-fluoride-exposed-swiss-albino-mice
#2
Shweta Sharma, Puneet Parashar, Subhasini Sharma, Kanta Prasad Sharma
Plant species rich in antioxidants (vitamins, flavonoids, lignans, and carotenoids) have been explored for complementary therapy of chronic diseases (cancers, coronary heart disease) and mitigation of pollutant toxicity. This article investigates their ameliorative role on selective hematological and serum biochemical parameters in fluoride-exposed (190 mg/kg body weight) Swiss albino mice pretreated with the antioxidant-rich diet supplements tomato puree (with and without peels), spirulina (cyanobacteria), and lycopene (present in tomato) for 45 days prior to entry into experimental protocol...
January 16, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29331737/hematology-reference-intervals-for-neonatal-holstein-calves
#3
Nikolaos Panousis, Nektarios Siachos, Georgios Kitkas, Emmanouil Kalaitzakis, Maria Kritsepi-Konstantinou, Georgios E Valergakis
Data regarding hematologic reference intervals (RI) for neonatal calves have not been published yet. The aims of this study were: a) to establish hematology RIs for neonatal Holstein calves, b) to compare them with the RIs for lactating cows, and c) to investigate the relationship of age and gender with the hematologic profile of calves. Two-hundred and fifty-four clinically healthy Holstein calves (1-9days old, from 30 farms) and 82 healthy Holstein cows (between 30 and 150days in milk, from 10 farms) were blood sampled once for a complete blood count evaluation, using the ADVIA 120 hematology analyzer...
January 9, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29325918/bioactive-fraction-of-rhodiola-algida-against-chronic-hypoxia-induced-pulmonary-arterial-hypertension-and-its-anti-proliferation-mechanism-in-rats
#4
Xingmei Nan, Shanshan Su, Ke Ma, Xiaodong Ma, Ximeng Wang, Dongzhu Zhaxi, Rili Ge, Zhanqiang Li, Dianxiang Lu
BACKGROUND: Rhodiola algida has long been used to prevent acute and chronic altitude sickness. In our previous study, we screened for a bioactive fraction from R. algida. However, the effects and mechanisms of this bioactive fraction on chronic hypoxia-induced pulmonary arterial hypertension remain to be elucidated. OBJECTIVE: The aim of this study was to determine the effect of bioactive fraction from R. algida (ACRT) on chronic hypoxia-induced pulmonary arterial hypertension (HPAH) and to understand the possible mechanism of its pharmacodynamic actions...
January 8, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29321527/a-hospital-based-retrospective-study-of-factors-influencing-therapeutic-leukapheresis-in-patients-presenting-with-hyperleukocytic-leukaemia
#5
Yanxia Jin, Shishang Guo, Qin Cui, Sichao Chen, Xiaoping Liu, Yongchang Wei, Yunbao Pan, Liang Tang, Tingting Huang, Hui Shen, Guanghui Xu, Xuelan Zuo, Shangqin Liu, Hui Xiao, Fei Chen, Fayun Gong, Fuling Zhou
Therapeutic leukapheresis is a rapid and effective method to reduce early mortality of patients with hyperleukocytic leukaemia (HLL). However, few studies on factors influencing the efficiency have been reported. In this study, 67 cases who underwent leukapheresis were retrospectively analysed and factors related to the collection efficiency of leukapheresis (CEWBC) were also evaluated. Paired t test showed that there was a significant decrease in statistics of white blood cell (WBC) counts after apheresis...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29313367/lecithin-coated-gold-nanoflowers-gnfs-for-ct-scan-imaging-applications-and-biochemical-parameters-in-vitro-and-in-vivo-studies
#6
Farooq Aziz, Khizra Bano, Ahmad Hassan Siddique, Sadia Zafar Bajwa, Aalia Nazir, Anam Munawar, Ayesha Shaheen, Madiha Saeed, Muhammad Afzal, M Zubair Iqbal, Aiguo Wu, Waheed S Khan
We report a novel strategy for the fabrication of lecithin-coated gold nanoflowers (GNFs) via single-step design for CT imaging application. Field-emission electron microscope confirmed flowers like morphology of the as-synthesized nanostructures. Furthermore, these show absorption peak in near-infrared (NIR) region at λmax 690 nm Different concentrations of GNFs are tested as a contrast agent in CT scans at tube voltage 135 kV and tube current 350 mA. These results are compared with same amount of iodine at same CT scan parameters...
January 9, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29303769/reference-intervals-for-biochemical-haemostatic-and-haematological-parameters-in-healthy-chinese-women-during-early-and-late-pregnancy
#7
Yi Jin, Jie Lu, Hong Jin, Chunrong Fei, Xinyou Xie, Jun Zhang
BACKGROUND: Variations in maternal blood parameters, which are mostly induced by the physiological changes that occur during pregnancy, have been reported in different gestational periods. The use of the established reference intervals for healthy adult females leads to the misclassification of healthy pregnant women as abnormal. Our aim was to establish appropriate reference intervals for biochemical, haematological and haemostatic parameters in the first and third trimesters of pregnancy...
January 5, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29296956/quantification-and-three-dimensional-microanatomical-organization-of-the-bone-marrow
#8
REVIEW
Cesar Nombela-Arrieta, Markus G Manz
Bone marrow (BM) constitutes one of the largest organs in mice and humans, continuously generating, in a highly regulated manner, red blood cells, platelets, and white blood cells that together form the majority of cells of the body. In this review, we provide a quantitative overview of BM cellular composition, we summarize emerging knowledge on its structural organization and cellular niches, and we argue for the need of multidimensional approaches such as recently developed imaging techniques to uncover the complex spatial logic that underlies BM function in health and disease...
February 14, 2017: Blood Advances
https://www.readbyqxmd.com/read/29295649/is-bone-marrow-examination-always-necessary-to-establish-the-diagnosis-of-myelodysplastic-syndromes-a-proposed-non-invasive-diagnostic-model
#9
Howard S Oster, Gal Carmi, Alex Kolomansky, Erel Joffe, Irit Kaye, Ilya Kirgner, Uri Greenbaum, Doron Comaneshter, Moshe Mittelman
A non-invasive myelodysplastic syndromes (MDS) diagnostic model would allow for care while avoiding invasive bone marrow examinations (BME). BME-established MDS patients were compared to non-MDS (BME-excluded) patients. Variables (gender, age, hemoglobin (Hb), mean red blood cell corpuscular volume (MCV), platelet (PLT), and white blood cell (WBC)) were combined with multivariate logistic regression; a probability score (Y) was calculated. MDS (n = 48) and non-MDS (n = 63) patients were used to establish the model...
January 2, 2018: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29261747/sex-specific-reference-intervals-of-hematologic-and-biochemical-analytes-in-sprague-dawley-rats-using-the-nonparametric-rank-percentile-method
#10
Qili He, Guoming Su, Keliang Liu, Fangcheng Zhang, Yong Jiang, Jun Gao, Lida Liu, Zhongren Jiang, Minwu Jin, Huiping Xie
BACKGROUND: Hematologic and biochemical analytes of Sprague-Dawley rats are commonly used to determine effects that were induced by treatment and to evaluate organ dysfunction in toxicological safety assessments, but reference intervals have not been well established for these analytes. Reference intervals as presently defined for these analytes in Sprague-Dawley rats have not used internationally recommended statistical method nor stratified by sex. Thus, we aimed to establish sex-specific reference intervals for hematologic and biochemical parameters in Sprague-Dawley rats according to Clinical and Laboratory Standards Institute C28-A3 and American Society for Veterinary Clinical Pathology guideline...
2017: PloS One
https://www.readbyqxmd.com/read/29214822/meeting-sleep-guidelines-is-associated-with-better-health-related-quality-of-life-and-reduced-premature-all-cause-mortality-risk
#11
Paul D Loprinzi, Chelsea Joyner
PURPOSE: To examine whether meeting sleep guidelines (7-9 hours/night) is associated with better health-related quality of life (HRQOL) and reduced all-cause mortality risk. DESIGN: Prospective cohort study. SETTING: 2005 to 2010 National Health and Nutrition Examination Survey. PARTICIPANTS: A total of 13 423 adults. MEASURES: Sleep duration and HRQOL were assessed from self-report; covariates assessed via survey, examination, and laboratory data; and mortality assessed through 2011 via matching from the National Death Index...
January 2018: American Journal of Health Promotion: AJHP
https://www.readbyqxmd.com/read/29209503/a-comparative-cross-sectional-study-of-some-hematological-parameters-of-hypertensive-and-normotensive-individuals-at-the-university-of-gondar-hospital-northwest-ethiopia
#12
Bamlaku Enawgaw, Nigist Adane, Betelihem Terefe, Fikir Asrie, Mulugeta Melku
Background: Hypertension is a major health problem worldwide. It can lead to cardiovascular disease and also leads to functional disturbances including hematological parameters. The abnormalities of haematological parameters may enhance an end-organ damage. Therefore, the aim of this study was to assess some hematological parameters of hypertensive individuals in comparison with normotensive individuals at University of Gondar hospital, northwest Ethiopia. Methods: A cross sectional comparative study was conducted from October to November 2015 on a total of 126 hypertensive and 126 normotensive individuals at University of Gondar Hospital...
2017: BMC Hematology
https://www.readbyqxmd.com/read/29207767/clinical-utility-of-blood-cell-histogram-interpretation
#13
REVIEW
E T Arun Thomas, S Bhagya, Abdul Majeed
An automated haematology analyser provides blood cell histograms by plotting the sizes of different blood cells on X-axis and their relative number on Y-axis. Histogram interpretation needs careful analysis of Red Blood Cell (RBC), White Blood Cell (WBC) and platelet distribution curves. Histogram analysis is often a neglected part of the automated haemogram which if interpreted well, has significant potential to provide diagnostically relevant information even before higher level investigations are ordered...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29175517/design-and-simulation-of-a-microfluidic-device-for-acoustic-cell-separation
#14
Amir Shamloo, Miad Boodaghi
Experimental acoustic cell separation methods have been widely used to perform separation for different types of blood cells. However, numerical simulation of acoustic cell separation has not gained enough attention and needs further investigation since by using numerical methods, it is possible to optimize different parameters involved in the design of an acoustic device and calculate particle trajectories in a simple and low cost manner before spending time and effort for fabricating these devices. In this study, we present a comprehensive finite element-based simulation of acoustic separation of platelets, red blood cells and white blood cells, using standing surface acoustic waves (SSAWs)...
November 21, 2017: Ultrasonics
https://www.readbyqxmd.com/read/29171038/effects-of-exposure-to-electromagnetic-field-from-915%C3%A2-mhz-radiofrequency-identification-system-on-circulating-blood-cells-in-the-healthy-adult-rat
#15
Hye Sun Kim, Jae Sung Park, Yeung-Bae Jin, Hyung Do Choi, Jong Hwa Kwon, Jeong-Ki Pack, Nam Kim, Young Hwan Ahn
We investigated whether exposure to the 915 MHz radiofrequency identification (RFID) signal affected circulating blood cells in rats. Sprague-Dawley rats were exposed to RFID at a whole-body specific absorption rate of 2 W/kg for 8 h per day, 5 days per week, for 2 weeks. Complete blood counts were performed after RFID exposure, and the CD4(+) /CD8(+) ratio was determined by flow cytometry. The number of red blood cells (RBCs) and the values of hemoglobin, hematocrit, and RBC indices were increased in the RFID-exposed group compared with those in the cage-control and sham-exposed groups (P < 0...
November 24, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/29159177/effectiveness-of-chinese-herbal-medicine-combined-with-antibiotics-for-extensively-drug-resistant-enterobacteria-and-nonfermentative-bacteria-infection-real-life-experience-in-a-retrospective-cohort
#16
Yangping Cai, Qing Zhang, Yuefeng Fu, Li Li, Ning Zhao, Aiping Lu, Qingquan Liu, Miao Jiang
Chinese herbal medicines (CHMs) have been successfully used in the treatment of infectious diseases, yet the effectiveness of CHMs for extensively drug-resistant enterobacteria (XDRE) infection remains unclear. Herein we developed a retrospective multicenter study including 766 patients with XDRE and nonfermentative bacteria (NFB) infection to investigate the effectiveness of CHMs combined with antibiotics in the treatment of XDRE infections in clinical daily practice in a cohort of patients and compared the regular antibiotics monotherapy...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29142420/exploring-the-cytotoxic-potential-of-triterpenoids-enriched-fraction-of-bacopa-monnieri-by-implementing-in-vitro-in-vivo-and-in-silico-approaches
#17
Md Nasar Mallick, Washim Khan, Rabea Parveen, Sayeed Ahmad, Sadaf, Mohammad Zeeshan Najm, Istaq Ahmad, Syed Akhtar Husain
Background: Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objectives: This work investigated anticancer activity of bioactive fraction of BM. Materials and Methods: The hydroalcoholic extract of BM was fractionated with different solvent, namely, hexane, dichloromethane (DCM), acetone, methanol, and water...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29141658/thioredoxin-mitigates-radiation-induced-hematopoietic-stem-cell-injury-in-mice
#18
Pasupathi Sundaramoorthy, Qinhong Wang, Zhihong Zheng, Yiqun Jiao, Benny J Chen, Phuong L Doan, Nelson J Chao, Yubin Kang
BACKGROUND: Radiation exposure poses a significant threat to public health. Hematopoietic injury is one of the major manifestations of acute radiation sickness. Protection and/or mitigation of hematopoietic stem cells (HSCs) from radiation injury is an important goal in the development of medical countermeasure agents (MCM). We recently identified thioredoxin (TXN) as a novel molecule that has marked protective and proliferative effects on HSCs. In the current study, we investigated the effectiveness of TXN in rescuing mice from a lethal dose of total body radiation (TBI) and in enhancing hematopoietic reconstitution following a lethal dose of irradiation...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29107162/artificial-stroke-clots-how-wide-is-the-gap-to-the-real-world
#19
Maria Berndt, Sascha Prothmann, Christian Maegerlein, Paul Oberdieck, Claus Zimmer, Barbara Hegge, Jaroslav Pelisek, Lucas Schirmer, Holger Poppert, Tobias Boeckh-Behrens
OBJECTIVE: Especially since the establishment of mechanical thrombectomy as part of the standard stroke therapy, artificial thrombi have become important in the training of interventionalists as well as for the development and testing of devices. So far, these in vitro clots lack direct comparisons with ex vivo thrombi. We therefore compared the histological appearance of dynamically produced clots with that of stroke thrombi acquired during mechanical recanalization. METHODS: Thrombi of 145 consecutive stroke patients with large-vessel occlusions were histologically compared with 10 artificial clots, dynamically created from human blood and pig's blood using a Chandler loop system...
October 26, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29101675/red-blood-cell-distribution-width-a-potential-laboratory-parameter-for-monitoring-inflammation-in-rheumatoid-arthritis
#20
Yujue He, Can Liu, Zhiyong Zeng, Weilin Ye, Jinpiao Lin, Qishui Ou
Correlation analysis of red blood cell distribution width (RDW) and C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), tumor necrosis factor α (TNF-α), interleukin (IL)-6, and IL-10 in rheumatoid arthritis (RA) to investigate whether RDW can serve as a potential parameter for indicating inflammation in RA patients. A total of 670 RA patients from October 2014 to April 2016 were enrolled in our study. The white blood cell (WBC), red blood cell (RBC), platelet (PLT), hemoglobin (HGB), RDW, CRP, and ESR in peripheral blood of patients with RA were retrospectively analyzed...
November 3, 2017: Clinical Rheumatology
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