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Sophia Kelaini, Marta Vilà-González, Rachel Caines, David Campbell, Magdalini Eleftheriadou, Marianna Tsifaki, Corey Magee, Amy Cochrane, Karla O'Neill, Chunbo Yang, Alan W Stitt, Lingfang Zeng, David J Grieve, Andriana Margariti
The fight against vascular disease requires functional endothelial cells (ECs) which could be provided by differentiation of induced Pluripotent Stem Cells (iPS Cells) in great numbers for use in the clinic. However, the great promise of the generated ECs (iPS-ECs) in therapy is often restricted due to the challenge in iPS-ECs preserving their phenotype and function. We identified that Follistatin-Like 3 (FSTL3) is highly expressed in iPS-ECs, and, as such, we sought to clarify its possible role in retaining and improving iPS-ECs function and phenotype, which are crucial in increasing the cells' potential as a therapeutic tool...
March 23, 2018: Stem Cells
Richard G James, Stephen R Reeves, Kaitlyn A Barrow, Maria P White, Veronika A Glukhova, Candace Haghighi, Dana Seyoum, Jason S Debley
RATIONALE: Bronchial epithelial cells (BECs) from healthy children inhibit human lung fibroblast (HLF) expression of collagen and fibroblast-myofibroblast transition (FMT) while asthmatic BECs do so less effectively, suggesting diminished epithelial-derived regulatory factors contribute to airway remodeling. Preliminary data demonstrated secretion of the activin A-inhibitor FSTL3 by healthy BECs was greater than that by asthmatic BECs. OBJECTIVES: Determine the relative secretion of FSTL3 and activin A by asthmatic and healthy BECs and if FSTL3 inhibits FMT...
February 2, 2018: American Journal of Respiratory Cell and Molecular Biology
Jaana Nevalainen, Teemu Korpimaki, Heikki Kouru, Mikko Sairanen, Markku Ryynanen
OBJECTIVE: To develop a predictive risk model for early-onset pre-eclampsia (EO-PE) using maternal characteristics, combined screening markers, previously reported biomarkers for PE and mean arterial pressure (MAP). METHODS: This retrospective study was conducted at Oulu University hospital between 2006 and 2010. Maternal serum from first trimester combined screening was further analyzed for alpha fetoprotein (AFP), placental growth factor (PlGF), soluble tumor necrosis factor receptor-1 (sTNFR1), retinol binding protein-4 (RBP4), a disintegrin and metalloprotease-12 (ADAM12), soluble P-selectin (sP-selectin), follistatin like-3 (FSTL3), adiponectin, angiopoietin-2 (Ang-2) and sex hormone binding globulin (SHBG)...
October 2017: Metabolism: Clinical and Experimental
Beilei Yuan, Wei Wu, Minjian Chen, Hao Gu, Qiuqin Tang, Dan Guo, Ting Chen, Yiqiu Chen, Chuncheng Lu, Ling Song, Yankai Xia, Daozhen Chen, Virender K Rehan, Jiahao Sha, Xinru Wang
There is increasing concern that early-life exposure to endocrine disruptors affects male offspring reproduction. However, whether di-n-butyl phthalate (DBP), a widely used endocrine disruptor, has transgenerational effects and, if so, the exact underlying molecular mechanisms involved remain unknown. In our study, 5 of time-mated pregnant SD rats were exposed to 0 and 500 mg/kg DBP with corn oil as the vehicle via oral gavage from embryonic days (E8-E14). Epigenetic and metabolomic of testis were analyzed after post-natal 60 days...
August 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
Anjalee T Amarasekera, Bahador Assadi-Khansari, Saifei Liu, Marilyn Black, Greer Dymmott, Natasha M Rogers, Aaron L Sverdlov, John D Horowitz, Doan T M Ngo
INTRODUCTION: Vitamin D insufficiency, defined as 25-hydroxyvitamin D (25(OH)D) levels < 75nmol/L is associated with cardio-metabolic dysfunction. Vitamin D insufficiency is associated with inflammation and fibrosis, but it remains uncertain whether these anomalies are readily reversible. Therefore, we aimed to determine the effects of vitamin D supplementation on markers of: 1) nitric oxide (NO) signaling, 2) inflammation, and 3) fibrosis, in healthy volunteers with mild hypovitaminosis...
2017: PloS One
Jennifer Bolton, Emilie Montastier, Jérôme Carayol, Sophie Bonnel, Lucile Mir, Marie-Adeline Marques, Arne Astrup, Wim Saris, Jason Iacovoni, Nathalie Villa-Vialaneix, Armand Valsesia, Dominique Langin, Nathalie Viguerie
Context: Although calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals. Objective: To identify biomarkers of successful weight control during a dietary intervention (DI). Design, Setting, and Participants: Adipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay...
August 1, 2017: Journal of Clinical Endocrinology and Metabolism
Susan Kralisch, Annett Hoffmann, Nora Klöting, Anette Bachmann, Jürgen Kratzsch, Jens-Uwe Stolzenburg, Anja Dietel, Joachim Beige, Matthias Anders, Ingolf Bast, Matthias Blüher, Ming-Zhi Zhang, Raymond C Harris, Michael Stumvoll, Mathias Fasshauer, Thomas Ebert
Background: Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. Methods: Circulating FSTL3 was quantified by enzyme-linked immunosorbent assay in 581 patients with CKD covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5...
October 1, 2017: Nephrology, Dialysis, Transplantation
Mehrdad Namdari, Babak Negahdari, Mostafa Cheraghi, Hammed T Aiyelabegan, Ali Eatmadi
Cardiac failure occurs when heart is unable to pump sufficiently to maintain blood flow to meet the body's needs. The aim of this work is to detect highly expressed genes: follistatin-related protein 1 (FSTL1) in heart failure within 30 minutes, using gold nanoparticles. Gold nanoparticles were prepared by citrate reduction of HAuCl4 3H2 O; probe sequence was designed based on the FSTL1 gene region. Preparation of gold nanoprobes (AuNPs) proceeded by treating all the containers with DEPC-treated water, followed by reduction and conjugation...
March 2017: Journal of Microencapsulation
Henrique L Couto, Marcelo A Buzelin, Nivaldo H Toppa, Enrrico Bloise, Alberto J Wainstein, Fernando M Reis
Follistatin-like 3 (FSTL3) binds and inactivates activin, a growth factor involved with cell growth and differentiation. We have previously shown FSTL3 overexpression in invasive breast cancers, but its clinical relevance remained unexplored. Here we evaluate FSTL3 as a prognostic tool and its relation with clinical and pathological features of breast cancer. A cohort of 154 women diagnosed with invasive breast cancer between 2008 and 2012 was followed up for 5 years. Tumor samples were processed by immunohistochemistry to detect FSTL3 expression in tumor epithelium...
June 27, 2017: Oncotarget
Signe Altmäe, Maria Teresa Segura, Francisco J Esteban, Sabine Bartel, Pilar Brandi, Martin Irmler, Johannes Beckers, Hans Demmelmair, Carmen López-Sabater, Berthold Koletzko, Susanne Krauss-Etschmann, Cristina Campoy
Maternal obesity has a major impact on pregnancy outcomes. There is growing evidence that maternal obesity has a negative influence on placental development and function, thereby adversely influencing offspring programming and health outcomes. However, the molecular mechanisms underlying these processes are poorly understood. We analysed ten term placenta's whole transcriptomes in obese (n = 5) and normal weight women (n = 5), using the Affymetrix microarray platform. Analyses of expression data were carried out using non-parametric methods...
2017: PloS One
Ayse Yasemin Karageyim Karsidag, Yunus Emre Purut, Esra Esim Buyukbayrak, Asuman Orcun, Mehmet Menke
PURPOSE: The purpose of this study is to determine whether the first trimester maternal serum levels of follistatin like 3 (FSTL3) are altered in patients who develop gestational diabetes mellitus (GDM). METHODS: This is a prospective nested case-control study that included 170 singleton pregnant women recruited in their first trimester. All women were followed up until the delivery and 144 of them completed the study. The maternal serum levels of FSTL3 were measured at 11-14 weeks of gestation...
September 29, 2016: Journal of Maternal-fetal & Neonatal Medicine
O Brew, M H F Sullivan, A Woodman
Pre-eclampsia (PE) is a serious multi-factorial disorder of human pregnancy. It is associated with changes in the expression of placental genes. Recent transcription profiling of placental genes with microarray analyses have offered better opportunities to define the molecular pathology of this disorder. However, the extent to which placental gene expression changes in PE is not fully understood. We conducted a systematic review of published PE and normal pregnancy (NP) control placental RNA microarrays to describe the similarities and differences between NP and PE placental gene expression, and examined how these differences could contribute to the molecular pathology of the disease...
2016: PloS One
Maryam Pashaiasl, Mansour Ebrahimi, Esmaeil Ebrahimie
Diminished ovarian reserve (DOR) is one of the reasons for infertility that not only affects both older and young women. Ovarian reserve assessment can be used as a new prognostic tool for infertility treatment decision making. Here, up- and down-regulated gene expression profiles of granulosa cells were analysed to generate a putative interaction map of the involved genes. In addition, gene ontology (GO) analysis was used to get insight intol the biological processes and molecular functions of involved proteins in DOR...
September 2016: Molecular Biology Reports
Gang Liu, Si Chen, Shucai Deng, Xinlong Ma, Yonghong Hao, Guoyun Bu
OBJECTIVE: Follistatin-like 3 (FSTL3), a circulating glycoprotein, is correlated with obesity and inflammation, which are potential mechanisms of osteoarthritis (OA). This study aims to determine the correlation of FSTL3 concentrations in serum and synovial fluid (SF) with the radiographic severity of OA. METHODS: This study consisted of 200 patients with knee OA and 148 healthy controls. The radiological grading of OA in the knee was performed in accordance with Kellgren-Lawrence (KL) grading system...
2015: International Journal of Clinical and Experimental Medicine
Melissa L Brown, Danielle Andrzejewski, Amy Burnside, Alan L Schneyer
Diabetes results from inadequate β-cell number and/or function to control serum glucose concentrations so that replacement of lost β-cells could become a viable therapy for diabetes. In addition to embryonic stem cell sources for new β-cells, evidence for transdifferentiation/reprogramming of non-β-cells to functional β-cells is accumulating. In addition, de-differentiation of β-cells observed in diabetes and their subsequent conversion to α-cells raises the possibility that adult islet cell fate is malleable and controlled by local hormonal and/or environmental cues...
March 2016: Endocrinology
Xiaohong Wang, Liyin Shi, Zhe Han, Baoshan Liu
Mesangial cells (MCs) proliferation and extracellular matrix (ECM) accumulation are early features of diabetic nephropathy. Follistatin-like 3 (FSTL3), a member of follistatin family, has been shown to regulate insulin and glucagon sensitivities in diet-induced obesity and insulin resistance. However, the role of FSTL3 in diabetic nephropathy is still unclear. Therefore, in this study, we investigated the effects of FSTL3 on cell proliferation and ECM accumulation expression in rat MCs cultured under high glucose, and elucidated the underlying mechanism...
2015: International Journal of Clinical and Experimental Medicine
Michael Larsen, Sudha Agarwal
No abstract text is available yet for this article.
October 2015: Plastic and Reconstructive Surgery
Tea Kaartokallio, Alejandra Cervera, Anjuska Kyllönen, Krista Laivuori, Juha Kere, Hannele Laivuori
Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls...
September 21, 2015: Scientific Reports
Danielle Andrzejewski, Melissa L Brown, Nathan Ungerleider, Amy Burnside, Alan L Schneyer
TGFβ superfamily ligands, receptors, and second messengers, including activins A and B, have been identified in pancreatic islets and proposed to have important roles regulating development, proliferation, and function. We previously demonstrated that Fstl3 (an antagonist of activin activity) null mice have larger islets with β-cell hyperplasia and improved glucose tolerance and insulin sensitivity in the absence of altered β-cell proliferation. This suggested the hypothesis that increased activin signaling influences β-cell expansion by destabilizing the α-cell phenotype and promoting transdifferentiation to β-cells...
July 2015: Endocrinology
J Nam, P Perera, R Gordon, Y H Jeong, A D Blazek, D G Kim, B C Tee, Z Sun, T D Eubank, Y Zhao, B Lablebecioglu, S Liu, A Litsky, N L Weisleder, B S Lee, T Butterfield, A L Schneyer, S Agarwal
Exercise is vital for maintaining bone strength and architecture. Follistatin-like 3 (FSTL3), a member of follistatin family, is a mechanosensitive protein upregulated in response to exercise and is involved in regulating musculoskeletal health. Here, we investigated the potential role of FSTL3 in exercise-driven bone remodeling. Exercise-dependent regulation of bone structure and functions was compared in mice with global Fstl3 gene deletion (Fstl3-/-) and their age-matched Fstl3+/+ littermates. Mice were exercised by low-intensity treadmill walking...
September 2015: Bone
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