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Susan Kralisch, Annett Hoffmann, Nora Klöting, Anette Bachmann, Jürgen Kratzsch, Jens-Uwe Stolzenburg, Anja Dietel, Joachim Beige, Matthias Anders, Ingolf Bast, Matthias Blüher, Ming-Zhi Zhang, Raymond C Harris, Michael Stumvoll, Mathias Fasshauer, Thomas Ebert
Background.: Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. Methods.: Circulating FSTL3 was quantified by enzyme-linked immunosorbent assay in 581 patients with CKD covering the whole spectrum of estimated glomerular filtration rate (eGFR) categories from G1 to G5...
March 3, 2017: Nephrology, Dialysis, Transplantation
Mehrdad Namdari, Babak Negahdari, Mostafa Cheraghi, Hammed T Aiyelabegan, Ali Eatmadi
Cardiac failure occurs when heart is unable to pump sufficiently to maintain blood flow to meet the body's needs. The aim of this work is to detect highly expressed genes: follistatin-related protein 1 (FSTL1) in heart failure within 30 minutes, using gold nanoparticles. Gold nanoparticles were prepared by citrate reduction of HAuCl4 3H2O; probe sequence was designed based on the FSTL1 gene region. Preparation of gold nanoprobes (AuNPs) proceeded by treating all the containers with DEPC-treated water, followed by reduction and conjugation...
March 7, 2017: Journal of Microencapsulation
Henrique L Couto, Marcelo A Buzelin, Nivaldo H Toppa, Enrrico Bloise, Alberto J Wainstein, Fernando M Reis
Follistatin-like 3 (FSTL3) binds and inactivates activin, a growth factor involved with cell growth and differentiation. We have previously shown FSTL3 overexpression in invasive breast cancers, but its clinical relevance remained unexplored. Here we evaluate FSTL3 as a prognostic tool and its relation with clinical and pathological features of breast cancer. A cohort of 154 women diagnosed with invasive breast cancer between 2008 and 2012 was followed up for 5 years. Tumor samples were processed by immunohistochemistry to detect FSTL3 expression in tumor epithelium...
February 2, 2017: Oncotarget
Signe Altmäe, Maria Teresa Segura, Francisco J Esteban, Sabine Bartel, Pilar Brandi, Martin Irmler, Johannes Beckers, Hans Demmelmair, Carmen López-Sabater, Berthold Koletzko, Susanne Krauss-Etschmann, Cristina Campoy
Maternal obesity has a major impact on pregnancy outcomes. There is growing evidence that maternal obesity has a negative influence on placental development and function, thereby adversely influencing offspring programming and health outcomes. However, the molecular mechanisms underlying these processes are poorly understood. We analysed ten term placenta's whole transcriptomes in obese (n = 5) and normal weight women (n = 5), using the Affymetrix microarray platform. Analyses of expression data were carried out using non-parametric methods...
2017: PloS One
Ayse Yasemin Karageyim Karsidag, Yunus Emre Purut, Esra Esim Buyukbayrak, Asuman Orcun, Mehmet Menke
PURPOSE: The purpose of this study is to determine whether the first trimester maternal serum levels of follistatin like 3 (FSTL3) are altered in patients who develop gestational diabetes mellitus (GDM). METHODS: This is a prospective nested case-control study that included 170 singleton pregnant women recruited in their first trimester. All women were followed up until the delivery and 144 of them completed the study. The maternal serum levels of FSTL3 were measured at 11-14 weeks of gestation...
September 29, 2016: Journal of Maternal-fetal & Neonatal Medicine
O Brew, M H F Sullivan, A Woodman
Pre-eclampsia (PE) is a serious multi-factorial disorder of human pregnancy. It is associated with changes in the expression of placental genes. Recent transcription profiling of placental genes with microarray analyses have offered better opportunities to define the molecular pathology of this disorder. However, the extent to which placental gene expression changes in PE is not fully understood. We conducted a systematic review of published PE and normal pregnancy (NP) control placental RNA microarrays to describe the similarities and differences between NP and PE placental gene expression, and examined how these differences could contribute to the molecular pathology of the disease...
2016: PloS One
Maryam Pashaiasl, Mansour Ebrahimi, Esmaeil Ebrahimie
Diminished ovarian reserve (DOR) is one of the reasons for infertility that not only affects both older and young women. Ovarian reserve assessment can be used as a new prognostic tool for infertility treatment decision making. Here, up- and down-regulated gene expression profiles of granulosa cells were analysed to generate a putative interaction map of the involved genes. In addition, gene ontology (GO) analysis was used to get insight intol the biological processes and molecular functions of involved proteins in DOR...
September 2016: Molecular Biology Reports
Gang Liu, Si Chen, Shucai Deng, Xinlong Ma, Yonghong Hao, Guoyun Bu
OBJECTIVE: Follistatin-like 3 (FSTL3), a circulating glycoprotein, is correlated with obesity and inflammation, which are potential mechanisms of osteoarthritis (OA). This study aims to determine the correlation of FSTL3 concentrations in serum and synovial fluid (SF) with the radiographic severity of OA. METHODS: This study consisted of 200 patients with knee OA and 148 healthy controls. The radiological grading of OA in the knee was performed in accordance with Kellgren-Lawrence (KL) grading system...
2015: International Journal of Clinical and Experimental Medicine
Melissa L Brown, Danielle Andrzejewski, Amy Burnside, Alan L Schneyer
Diabetes results from inadequate β-cell number and/or function to control serum glucose concentrations so that replacement of lost β-cells could become a viable therapy for diabetes. In addition to embryonic stem cell sources for new β-cells, evidence for transdifferentiation/reprogramming of non-β-cells to functional β-cells is accumulating. In addition, de-differentiation of β-cells observed in diabetes and their subsequent conversion to α-cells raises the possibility that adult islet cell fate is malleable and controlled by local hormonal and/or environmental cues...
March 2016: Endocrinology
Xiaohong Wang, Liyin Shi, Zhe Han, Baoshan Liu
Mesangial cells (MCs) proliferation and extracellular matrix (ECM) accumulation are early features of diabetic nephropathy. Follistatin-like 3 (FSTL3), a member of follistatin family, has been shown to regulate insulin and glucagon sensitivities in diet-induced obesity and insulin resistance. However, the role of FSTL3 in diabetic nephropathy is still unclear. Therefore, in this study, we investigated the effects of FSTL3 on cell proliferation and ECM accumulation expression in rat MCs cultured under high glucose, and elucidated the underlying mechanism...
2015: International Journal of Clinical and Experimental Medicine
Michael Larsen, Sudha Agarwal
No abstract text is available yet for this article.
October 2015: Plastic and Reconstructive Surgery
Tea Kaartokallio, Alejandra Cervera, Anjuska Kyllönen, Krista Laivuori
Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls...
September 21, 2015: Scientific Reports
Danielle Andrzejewski, Melissa L Brown, Nathan Ungerleider, Amy Burnside, Alan L Schneyer
TGFβ superfamily ligands, receptors, and second messengers, including activins A and B, have been identified in pancreatic islets and proposed to have important roles regulating development, proliferation, and function. We previously demonstrated that Fstl3 (an antagonist of activin activity) null mice have larger islets with β-cell hyperplasia and improved glucose tolerance and insulin sensitivity in the absence of altered β-cell proliferation. This suggested the hypothesis that increased activin signaling influences β-cell expansion by destabilizing the α-cell phenotype and promoting transdifferentiation to β-cells...
July 2015: Endocrinology
J Nam, P Perera, R Gordon, Y H Jeong, A D Blazek, D G Kim, B C Tee, Z Sun, T D Eubank, Y Zhao, B Lablebecioglu, S Liu, A Litsky, N L Weisleder, B S Lee, T Butterfield, A L Schneyer, S Agarwal
Exercise is vital for maintaining bone strength and architecture. Follistatin-like 3 (FSTL3), a member of follistatin family, is a mechanosensitive protein upregulated in response to exercise and is involved in regulating musculoskeletal health. Here, we investigated the potential role of FSTL3 in exercise-driven bone remodeling. Exercise-dependent regulation of bone structure and functions was compared in mice with global Fstl3 gene deletion (Fstl3-/-) and their age-matched Fstl3+/+ littermates. Mice were exercised by low-intensity treadmill walking...
September 2015: Bone
Claus Brandt, Rasmus Hvass Hansen, Jakob Bondo Hansen, Caroline Holkmann Olsen, Pia Galle, Thomas Mandrup-Poulsen, Julie Gehl, Bente Klarlund Pedersen, Pernille Hojman
OBJECTIVE: Follistatin-like 3 (fstl3), a natural inhibitor of members of the TGF-β family, increases during resistance training in human plasma. Fstl3 primarily binds myostatin and activin A, and thereby inhibits their functions. We hypothesize that blocking myostatin and activin A signalling through systemic fstl3 over-expression protects against diet-induced obesity and insulin resistance. METHODS: Fstl3 was over-expressed by DNA electrotransfer in tibialis anterior, quadriceps and gastrocnemius muscles in female C57BL/C mice, and the mice were subsequently randomized to chow or high-fat feeding...
February 2015: Metabolism: Clinical and Experimental
Claus Brandt, Maria Pedersen, Anders Rinnov, Anne S Andreasen, Kirsten Møller, Pernille Hojman, Bente K Pedersen, Peter Plomgaard
BACKGROUND: Rodent models suggest that follistatin-like 3 (fstl3) is associated with diabetes and obesity. In humans, plasma fstl3 is reduced with gestational diabetes. In vitro, TNF-α induces fstl3 secretion, which suggests a link to inflammation. OBJECTIVE: To elucidate the association between plasma fstl3 and obesity, insulin resistance, and low-grade inflammation in humans. STUDY DESIGN: Plasma fstl3 levels were determined in a cross-sectional study including three groups: patients with type 2 diabetes, impaired glucose tolerance, and healthy controls...
2014: Mediators of Inflammation
Silvia Näf, Xavier Escote, Mónica Ballesteros, Rosa Elena Yañez, Inmaculada Simón-Muela, Pilar Gil, Gerard Albaiges, Joan Vendrell, Ana Megia
CONTEXT: The Activin A-Follistatin system has emerged as an important regulator of lipid and glucose metabolism with possible repercussions on fetal growth. OBJECTIVE: To analyze circulating activin A, follistatin and follistatin-like-3 (FSTL3) levels and their relationship with glucose metabolism in pregnant women and their influence on fetal growth and neonatal adiposity. DESIGN AND METHODS: A prospective cohort was studied comprising 207 pregnant women, 129 with normal glucose tolerance (NGT) and 78 with gestational diabetes mellitus (GDM) and their offspring...
2014: PloS One
Tomoyuki Masuda, Chie Sakuma, Atsuko Nagaoka, Toshiyuki Yamagishi, Shuichi Ueda, Takahiro Nagase, Hiroyuki Yaginuma
Follistatin-like 5 (Fstl5), a member of the follistatin family of genes, encodes a secretory glycoprotein. Previous studies revealed that other members of this family including Fstl1 and Fstl3 play an essential role in development, homeostasis, and congenital disorders. However, the in vivo function of Fstl5 is poorly understood. To gain insight into the function of Fstl5 in the mouse central nervous system, we examined the Fstl5 expression pattern in the adult mouse brain. The results of in situ hybridization analysis showed a highly restricted pattern of Fstl5, namely, with localization in the olfactory system, hippocampal CA3 area and granular cell layer of the cerebellum...
February 2014: Congenital Anomalies
Anna M Zawadzka, Birgit Schilling, Michael P Cusack, Alexandria K Sahu, Penelope Drake, Susan J Fisher, Christopher C Benz, Bradford W Gibson
Breast cancer is a heterogeneous disease whose molecular diversity is not well reflected in clinical and pathological markers used for prognosis and treatment selection. As tumor cells secrete proteins into the extracellular environment, some of these proteins reach circulation and could become suitable biomarkers for improving diagnosis or monitoring response to treatment. As many signaling pathways and interaction networks are altered in cancerous tissues by protein phosphorylation, changes in the secretory phosphoproteome of cancer tissues could reflect both disease progression and subtype...
April 2014: Molecular & Cellular Proteomics: MCP
Martha Behnke, Mark Reimers, Robert Fisher
Hepatocellular carcinoma (HCC) remains a difficult disease to study even after a decade of genomic analysis. Patient and disease heterogeneity, differences in statistical methods and multiple testing issues have resulted in a fragmented understanding of the molecular basis of tumor biology. Some researchers have suggested that HCC appears to share pathways with embryonic development. Therefore we generated targeted hypotheses regarding changes in developmental genes specific to the liver in HCV-cirrhosis and HCV-HCC...
September 1, 2012: Cancers
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