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Simona Cavalu, Jόszef Prokisch, Vasile Laslo, Simona Vicas
The main goal of this study was to synthesise and characterise different formulations based on alginate and alginate/chitosan microspheres containing nanoselenium (nano-Se) for controlled delivery applications. Nanosize elemental selenium was produced by using probiotic yogurt bacteria (Lactobacillus casei) in a fermentation procedure. The structural and morphological characterisation of the microspheres was performed by Fourier transform infrared (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis...
June 2017: IET Nanobiotechnology
José M Gallego-Escuredo, Maria Karuna Lamarca, Joan Villarroya, Joan C Domingo, Ma Gracia Mateo, Ma Del Mar Gutierrez, Francesc Vidal, Francesc Villarroya, Pere Domingo, Marta Giralt
BACKGROUND: Fibroblast growth factor-21 (FGF21) has emerged as an important regulator of glucose, lipid, and body weight homeostasis. However, recent experimental studies have reported that increased FGF21 levels may lead to bone loss. OBJECTIVE: To assess the relationship of serum FGF21 levels and altered bone homeostasis in HIV-1-infected patients. DESIGN: Cross-sectional study of 137 HIV-1-infected patients and 35 healthy controls conducted at the Hospital de la Santa Creu i Sant Pau, Barcelona...
June 2017: Metabolism: Clinical and Experimental
J Wang, W Xia, J Deng, X Xu, Y Shao, H Ding, Y Chen, J Liu, D Chen, X Ye, S Santoso
BACKGROUND: Cross-match-compatible platelets can improve corrected count increments (CCIs) in alloimmunised patients with transfusion refractoriness. However, only a few studies mentioned that the specificities of platelet-reactive alloantibodies can predict high reactivity in cross-match assays among these patients. METHODS: A total of 204 medical records of patients who were refractory to random single-donor apheresis platelets between January 2014 and December 2014 were enrolled...
May 18, 2017: Transfusion Medicine
Agustin Iskandar, Erlin Nursiloningrum, Maimun Zulhaidah Arthamin, Ery Olivianto, Mas Slamet Chandrakusuma
INTRODUCTION: Diagnosis of Tuberculosis (TB) in children is difficult because the clinical presentation is not specific, the chest X-ray interpretation has low accuracy and sputum sample is difficult to obtain. Antigen detection test such as rapid urine LAM is a non-invasive alternative for diagnosing TB . Lipoarabinomannan (LAM) is the main component of M.tuberculosis cell wall. AIM: To determine the diagnostic value of urinary LAM antigen for diagnosis of childhood TB...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
Delia Picone, Federica Donnarumma, Giarita Ferraro, Giovanni Gotte, Andrea Fagagnini, Giovanna Butera, Massimo Donadelli, Antonello Merlino
Cisplatin (CDDP) can form interprotein cross-links, leading to the formation of platinated oligomers. A dimer, a trimer and higher oligomers of bovine pancreatic ribonuclease (RNase A) obtained upon reaction with CDDP in 1:10 protein to metal ratio at 37°C have been previously characterized. Here, we verify the ability of carboplatin and oxaliplatin to induce RNase A oligomerization under the same experimental conditions. The amount of formed RNase A oligomers was compared with that obtained in the reaction of the protein with CDDP...
May 9, 2017: Journal of Inorganic Biochemistry
Karen Stroobants, Janet Reiko Kumita, Nicola Harris, Dimitri Chirgadze, Christopher M Dobson, Paula J Booth, Michele Vendruscolo
The propensity to misfold and self-assemble into stable aggregates is increasingly recognised as a common feature of protein molecules. Our understanding of this phenomenon and of its links with human disease has increased substantially over the last two decades. Studies thus far, however, have been almost exclusively focussed on cytosolic proteins, resulting in a lack of detailed information about the misfolding and aggregation of membrane proteins. As a consequence, although such proteins make up approximately 30% of the human proteome and have high propensities to aggregate, relatively little is known about the biophysical nature of their assemblies...
May 11, 2017: Biochemistry
Ivan Zaluzhnyy, Ruslan P Kurta, Alexander André, Oleg Gorobtsov, Max Rose, Petr Skopintsev, Ilya Besedin, Alexey V Zozulya, Michael Sprung, Frank Schreiber, Ivan A Vartanyants, Marcus Scheele
We show that the combination of X-ray scattering with a nanofocused beam and X-ray cross correlation analysis is an efficient means for the full structural characterization of mesocrystalline nanoparticle assemblies with a single experiment. We analyze several hundred diffraction patterns of individual sample locations, i.e. individual grains, to obtain a meaningful statistical distribution of the superlattice and atomic lattice ordering. Simultaneous small- and wide-angle X-ray scattering of the same sample location allows us to determine the structure and orientation of the superlattice as well as the angular correlation of the first two Bragg peaks of the atomic lattices, their orientation with respect to the superlattice, and the average orientational misfit due to local structural disorder...
May 9, 2017: Nano Letters
H Guo, Q Zhu, X Yu, S B Merugu, H B Mangukiya, N Smith, Z Li, B Zhang, H Negi, R Rong, K Cheng, Z Wu, D Li
The importance of the tumor microenvironment in targeted anticancer therapies has been well recognized. Various protein factors participate in the cross-talk between tumor cells and non-malignant cells. Anterior gradient-2 (AGR2) is overexpressed in diverse human adenocarcinomas and it exists in both intracellular and extracellular spaces. Although intracellular AGR2 has been intensively investigated, the function of secreted AGR2, especially its exact mechanism of action is still poorly understood. Here we report that the secreted AGR2 promotes the angiogenesis and the invasion of vascular endothelial cells and fibroblasts by enhancing the activities of vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2)...
May 8, 2017: Oncogene
Ghasem Bahlakeh, Bahram Ramezanzadeh
The influences of steel surface treatment by a novel cerium-lanthanum (Ce-La) nanofilm on the adhesion mechanism of an epoxy adhesive were studied through experimental and modeling approaches. The surface morphology and microstructure of the film deposited were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The surface free energy and work of adhesion values were evaluated through contact angle analysis...
May 8, 2017: ACS Applied Materials & Interfaces
Fábio A Araújo, Ana Martins, Nuno Alegrete, Laura D Howe, Raquel Lucas
BACKGROUND CONTEXT: In each specific habitual standing posture, gravitational forces determine the mechanical setting provided to skeletal structures. Bone quality and resistance to physical stress is highly determined by habitual mechanical stimulation. However, the relationship between bone properties and sagittal posture has never been studied in children. PURPOSE: To investigate the association between bone physical properties and sagittal standing postural patterns in 7-year-old children...
May 4, 2017: Spine Journal: Official Journal of the North American Spine Society
Siva Sankar Sana, Vijaya Kumar Naidu Boya
Herein, the authors developed a new and potential semi-interpenetrating polymer network (semi-IPN) hydrogels of poly vinyl alcohol (PVA), acryl amide and diallyldimethyl ammonium chloride employing chemical cross-linker N, N'-methylene bisacrylamide (NNMBA) and ammonium persulphate as an initiator by radical polymerisation. To analyse the copolymer formation between two monomers and IPN cross-linking reaction, the resulting hydrogel was characterised by Fourier transform infrared spectroscopy and the surface morphology was analysed using scanning electron microscopy...
February 2017: IET Nanobiotechnology
Katrin Unger, Paul Salzmann, Cecilia Masciullo, Marco Cecchini, Georg Koller, Anna Maria Coclite
A novel multiresponsive hydrogel has been synthesized by initiated chemical vapor deposition (iCVD). Hydrogels are known for their dynamic swelling response to aqueous environments. A chemical functionalization of the hydrogel surface was performed to add other stimuli-responsive functionalities and obtain a smart material that responds to two stimuli: light irradiation and exposure to aqueous environment. Modifying the hydrogel surface with solution-based methods is often problematic because of the damages caused by the permeation of solvents in the hydrogel...
May 12, 2017: ACS Applied Materials & Interfaces
Stefanie Schreiber, Grazyna Debska-Vielhaber, Susanne Abdulla, Judith Machts, Frank Schreiber, Siegfried Kropf, Peter Körtvelyessy, Sonja Körner, Katja Kollewe, Susanne Petri, Reinhard Dengler, Wolfram S Kunz, Peter J Nestor, Stefan Vielhaber
INTRODUCTION: We aimed to investigate whether sonographic peripheral cross-sectional nerve area (CSA) and progranulin (PGRN), a neuritic growth factor, are related to each other and whether they interact to predict clinical and paraclinical measures in amyotrophic lateral sclerosis (ALS). METHODS: We included 55 ALS patients who had forearm median and ulnar nerve CSA, cerebrospinal fluid (CSF) PGRN and serum PGRN measures available. CSF PGRN was normalized against the CSF/serum albumin ratio (Qalb )...
May 4, 2017: Muscle & Nerve
Silvana Cartaxo da Costa Urtiga, Camilla Aquino Azevedo de Lucena Gabi, Giovanna Rodrigues de Araújo Eleamen, Bartolomeu Santos Souza, Hilzeth de Luna Freire Pessôa, Henrique Rodrigues Marcelino, Elisângela Afonso de Moura Mendonça, Eryvaldo Sócrates Tabosa do Egito, Elquio Eleamen Oliveira
This work describes the preparation and evaluation of safe xylan-based microparticles prepared by cross-linking polymerization using sodium trimetaphosphate. The resulting microparticles were evaluated for morphology, particle size, polymer-cross-link agent interaction and in vitro toxicity. The microparticles showed narrow monodisperse size distributions with their mean sizes being between 3.5 µm and 12.5 µm in dried state. FT-IR analyses confirmed the interaction between sodium trimetaphosphate and xylan during the cross-linking process with formation of phosphate ester bonds...
May 4, 2017: Drug Development and Industrial Pharmacy
Sung Hyun Kim, Cheol Hwan Kim, Woo Jin Choi, Tae Gon Lee, Seong Keun Cho, Yong Suk Yang, Jae Heung Lee, Sang-Jin Lee
Carbon nanotube/polytetrafluoroethylene composite polymer targets are proposed for use in the fabrication of fluorocarbon thin films using the mid-frequency sputtering process. Fluorocarbon thin films deposited using carbon nanotube/polytetrafluoroethylene composite targets exhibit an amorphous phase with a smooth surface and show a high water contact angle, optical transmittance, and surface hardness. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy studies reveal that as the carbon nanotube concentration increased in the composite target, a carbon cross-linked structure was formed, which enhanced the film hardness and the modulus of the fluorocarbon thin film...
May 3, 2017: Scientific Reports
Jiangyu Yan, Thomas R Beattie, Adriana L Rojas, Kelly Schermerhorn, Tamzin Gristwood, Jonathan C Trinidad, Sonja V Albers, Pietro Roversi, Andrew F Gardner, Nicola G A Abrescia, Stephen D Bell
Since their initial characterization over 30 years ago, it has been believed that the archaeal B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit B-family replicative polymerases of eukaryotes. Here we reveal that the highly studied PolB1 from Sulfolobus solfataricus exists as a heterotrimeric complex in cell extracts. Two small subunits, PBP1 and PBP2, associate with distinct surfaces of the larger catalytic subunit and influence the enzymatic properties of the DNA polymerase...
May 2, 2017: Nature Communications
Sindy Lindner, Kai Gruhle, Rico Schmidt, Vasil M Garamus, Daniel Ramsbeck, Gerd Hause, Annette Meister, Andrea Sinz, Simon Drescher
In the present work, we describe the synthesis and the temperature-dependent behavior of photoreactive membrane lipids as well as their capability to study peptide/lipid interactions. The modified phospholipids contain an azide group either in the middle part or at the end of an alkyl chain and also differ in the linkage (ester vs ether) of the second alkyl chain. The temperature-dependent aggregation behavior of the azidolipids was studied using differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and small-angle X-ray scattering (SAXS)...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Alvin Ho-Ting Li, Ngan N Lam, Sonny Dhanani, Matthew Weir, Versha Prakash, Joseph Kim, Greg Knoll, Amit X Garg
BACKGROUND: Canada has low rates of deceased organ and tissue donation. Immigrants to Canada may differ in their registered support for deceased organ donation based on their country of origin. METHODS: We used linked administrative databases in Ontario (about 11 million residents aged ≥ 16 yr) to study the proportion of immigrants and long-term residents registered for deceased organ and tissue donation as of October 2013. We used modified Poisson regression to identify and quantify predictors of donor registration...
October 2016: CMAJ Open
Silvia Gonçalves Ricci Neri, André Bonadias Gadelha, Ana Luiza Matias Correia, Juscélia Cristina Pereira, Ana Cristina de David, Ricardo Moreno Lima
Increased plantar pressure has been found to be related with greater risk of falling. Although there is evidence suggesting that obesity is linked to foot disorders, the association between obesity and plantar pressure of older adults has been poorly investigated. The purpose of this study was to examine the association between obesity and plantar pressure distribution and to explore its relationship with body fat distribution. Two hundred and eleven elderly women took part in this cross-sectional study. Body mass index were taken for obesity classification...
April 19, 2017: Journal of Applied Biomechanics
Jun Sun, Lin Yang, Mengmeng Jiang, Yu Shi, Bin Xu, Hai-le Ma
Enzyme cross-linkers, such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and glutaraldehyde (GA), have been used to improve the stability of immobilized enzymes. We have developed a relatively stable and high-activity immobilized trypsin through EDC and GA cross-linking. Carboxymethyl chitosan (CM-CTS)-functionalized magnetic nanoparticles (Fe3O4@CM-CTS) were prepared, and characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis, X-ray diffraction, and transmission electron microscopy...
April 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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