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bombyx mori baculovirus

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https://www.readbyqxmd.com/read/28821766/protein-composition-analysis-of-polyhedra-matrix-of-bombyx-mori-nucleopolyhedrovirus-bmnpv-showed-powerful-capacity-of-polyhedra-to-encapsulate-foreign-proteins
#1
Zhong-Jian Guo, Meng-Han Yu, Xian-Yun Dong, Wei-Li Wang, Ting Tian, Xian-Yin Yu, Xu-Dong Tang
Polyhedra can encapsulate other proteins and have potential applications as protein stabilizers. The extremely stable polyhedra matrix may provide a platform for future engineered micro-crystal devices. However, the protein composition of the polyhedra matrix remains largely unknown. In this study, the occlusion-derived virus (ODV)-removed BmNPV polyhedra matrix fraction was subjected to SDS-PAGE and then an LC-ESI-MS/MS analysis using a Thermo Scientific Q Exactive mass spectrometer. In total, 28 host and 91 viral proteins were identified...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28797905/evidence-for-the-role-of-bmnpv-bm65-protein-in-the-repair-of-ultraviolet-induced-dna-damage
#2
Qi Tang, Peng Wu, Zhaoyang Hu, Yanhua Yang, Lipeng Qiu, Hanqing Liu, Shanying Zhu, Zhongjian Guo, Hengchuan Xia, Keping Chen, Guohui Li
It is unclear how, or to what extent, baculovirus DNA that has been damaged by ultraviolet (UV) light is repaired during infection and replication. In our previous study, expression of Bombyx mori nucleopolyhedrovirus (BmNPV) ORF Bm65, a homolog of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac79, correlated with decreased inactivation of virus by UV irradiation. In the current study, we accumulated more evidence pointing to a role for Bm65 in repair of UV-induced DNA damage. The localization of Bm65 was studied using enhanced green fluorescent protein (EGFP) fusion constructs expressed in BmN cells transfected with a Bm65 expression plasmid...
October 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28755911/itraq-proteomic-analysis-of-the-interactions-between-bombyx-mori-nuclear-polyhedrosis-virus-and-silkworm
#3
Wei-Tao Dong, Long-Fei Xiao, Jun-Jie Hu, Xin-Xu Zhao, Ji-Xing Liu, Yong Zhang
The silkworm hemolymph is an important defense system against bacteria and viruses. In this study, silkworms were infected with Bombyx mori nuclear polyhedrosis virus to investigate the subsequent immune response at the protein level. Proteomes were analyzed before and after infection using isobaric tags for relative and absolute quantitation and LC-MS. A total of 456 differentially expressed proteins were identified, of which 179 were upregulated and 193 were downregulated. Changes in expression were validated by western blot for several proteins...
July 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28648848/baculovirus-lef-11-nuclear-localization-signal-is-important-for-viral-dna-replication
#4
Tingting Chen, Zhanqi Dong, Nan Hu, Zhigang Hu, Feifan Dong, Yaming Jiang, Jun Li, Peng Chen, Cheng Lu, Minhui Pan
Baculovirus LEF-11 is a small nuclear protein that is involved in viral late gene transcription and DNA replication. However, the characteristics of its nuclear localization signal and its impact on viral DNA replication are unknown. In the present study, systemic bioinformatics analysis showed that the baculovirus LEF-11 contains monopartite and bipartite classical nuclear localization signal sequences (cNLSs), which were also detected in a few alphabaculovirus species. Localization of representative LEF-11 proteins of four baculovirus genera indicated that the nuclear localization characteristics of baculovirus LEF-11 coincided with the predicted results...
June 23, 2017: Virus Research
https://www.readbyqxmd.com/read/28630398/hcf-1-encoded-by-baculovirus-acmnpv-is-required-for-productive-nucleopolyhedrovirus-infection-of-non-permissive-tn368-cells
#5
Ami Tachibana, Rina Hamajima, Moe Tomizaki, Takuya Kondo, Yoshie Nanba, Michihiro Kobayashi, Hayato Yamada, Motoko Ikeda
Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in both Spodoptera frugiperda Sf21 and Trichoplusia ni Tn368 cells, whereas AcMNPV defective in hcf-1 (host cell-factor 1) gene productively infects only Sf21 cells, indicating that HCF-1 is indispensable for the AcMNPV productive infection of Tn368 cells. Here, we demonstrated that HCF-1 protein transiently expressed in Tn368 cells promotes the DNA synthesis of Hyphantria cunea MNPV (HycuMNPV), Orygia pseudotsugata MNPV and Bombyx mori NPV, which are normally unable to replicate in Tn368 cells...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28629377/quantitative-phosphoproteome-on-the-silkworm-bombyx-mori-cells-infected-with-baculovirus
#6
Jauharotus Shobahah, Shengjie Xue, Dongbing Hu, Cui Zhao, Ming Wei, Yanping Quan, Wei Yu
BACKGROUND: Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive. METHOD: Employing tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by high-resolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined...
June 19, 2017: Virology Journal
https://www.readbyqxmd.com/read/28393927/baculovirus-lef-11-hijack-host-atpase-atad3a-to-promote-virus-multiplication-in-bombyx-mori-cells
#7
Zhan-Qi Dong, Nan Hu, Fei-Fan Dong, Ting-Ting Chen, Ya-Ming Jiang, Peng Chen, Cheng Lu, Min-Hui Pan
Research on molecular mechanisms that viruses use to regulate the host apparatus is important in virus infection control and antiviral therapy exploration. Our previous research showed that the Bombyx mori nucleopolyhedrovirus (BmNPV) LEF-11 localized to dense regions of the cell nucleus and is required for viral DNA replication. Herein, we examined the mechanism of LEF-11 on BmNPV multiplication and demonstrated that baculovirus LEF-11 interacts with Bombyx mori ATAD3A and HSPD1 (HSP60) protein. Furthermore, we showed that LEF-11 has the ability to induce and up-regulate the expression of ATAD3A and HSPD1, phenomena that were both reversed upon knockdown of lef-11...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28077638/a-conserved-glycine-residue-is-required-for-proper-functioning-of-a-baculovirus-vp39-protein
#8
Susumu Katsuma, Ryuhei Kokusho
The baculovirus VP39 protein is a major nucleocapsid protein essential for viral propagation. However, the critical domains or residues of the VP39 protein have not yet been identified. Here, we performed mutagenesis experiments with Bombyx mori nucleopolyhedrovirus (BmNPV) using 5-bromo-2'-deoxyuridine and isolated a BmNPV mutant that produced fewer occlusion bodies than the wild-type virus. This mutant also produced fewer infectious budded viruses (BVs) than the wild-type virus in both cultured cells and B...
March 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27813251/functional-characterization-of-bacillus-thuringiensis-cry-toxin-receptors-explains-resistance-in-insects
#9
Shiho Tanaka, Haruka Endo, Satomi Adegawa, Shingo Kikuta, Ryoichi Sato
Bacillus thuringiensis produces Cry toxins, which are used as insecticides in sprays and in transgenic crops. However, little is known about the function of Cry toxin receptors and the mechanisms that determine their binding specificity and activity. In this study, the cRNAs of Bombyx mori ABC transporter C2 (BmABCC2), the toxin-binding region of cadherin-like receptor (BtR175-TBR), or aminopeptidase N1 (BmAPN1) were injected into Xenopus oocytes, and the Cry1Aa-dependent cation-selective pore formation activities of these receptors were analyzed using a two-electrode voltage clamp...
December 2016: FEBS Journal
https://www.readbyqxmd.com/read/27701142/caspase-1-from-the-silkworm-bombyx-mori-is-involved-in-bombyx-mori-nucleopolyhedrovirus-infection
#10
Qiang Wang, Xiaoli Ju, Liang Chen, Keping Chen
Caspase-1 is one of the effector caspases in mammals that plays a central role in apoptosis. However, the lepidopteran caspase-1, especially the Bombyx mori caspase-1 (Bm-caspase-1), has not been investigated in detail. In this study, Bm-caspase-1 was identified from an expressed sequence tag database in B. mori by BLAST search. The open reading frame of Bm-caspase-1 contained 879 nucleotides and encoded 293 amino acids with a predicted molecular mass of 33 kDa. Bm-caspase-1 contained two consensus amino acid motifs of caspase cleavage sites, DEGDA and TETDG...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27669694/production-of-human-type-ii-collagen-using-an-efficient-baculovirus-silkworm-multigene-expression-system
#11
Qi Qi, Lunguang Yao, Zhisheng Liang, Donghua Yan, Zhuo Li, Yadong Huang, Jingchen Sun
Human type II collagen is a macromolecular protein found throughout the human body. The baculovirus expression vector system is one of the most ideal systems for the routine production and display of recombinant eukaryotic proteins in insect, larvae, and mammalian cells. We use this system to express a full-length gene, human type II collagen cDNA (4257 bp), in cultured Spodoptera frugiperda 9 cells (Sf9), Bombyx mori cells, and silkworm larvae. In this study, the expression of human type II collagen gene in both insect cells and silkworm larvae was purified by nickel column chromatography, leading to 300-kDa bands in SDS-PAGE and western blotting indicative of collagen α-chains organized in a triple-helical structure...
December 2016: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/27640438/functional-characterization-of-bombyx-mori-nucleopolyhedrovirus-mutant-lacking-late-expression-factor-9
#12
Y Zhang, Y Shi, H Yu, J Li, Y Quan, T Shu, Z Nie, Y Zhang, W Yu
Baculoviridae is a family of invertebrate viruses with large double-stranded DNA genomes. Proteins encoded by some late expression factor (lef ) genes are involved in the regulation of viral gene expression. Lef-9 is one of four transcription-specific Lefs, which are components of the virus-encoded RNA polymerase, and can initiate and transcribe late and very late genes. As a multifunctional protein encoded by the Bombyx mori nucleopolyhedrovirus (BmNPV), Lef-9 may be involved in the regulation of viral propagation...
2016: Acta Virologica
https://www.readbyqxmd.com/read/27614700/bombyx-mori-nucleopolyhedrovirus-bm5-protein-regulates-progeny-virus-production-and-viral-gene-expression
#13
Ryuhei Kokusho, Yoshikazu Koh, Masaru Fujimoto, Toru Shimada, Susumu Katsuma
Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mori cultured cells and larvae, although viral genome replication was not affected. Bm5 disruption also caused aberrant expression of various viral genes at the very late stage of infection. Immunocytochemical analysis revealed that BM5 localized to the nuclear membrane...
November 2016: Virology
https://www.readbyqxmd.com/read/27489496/yorkie-facilitates-organ-growth-and-metamorphosis-in-bombyx
#14
Shumin Liu, Panli Zhang, Hong-Sheng Song, Hai-Sheng Qi, Zhao-Jun Wei, Guozheng Zhang, Shuai Zhan, Zhihong Liu, Sheng Li
The Hippo pathway, which was identified from genetic screens in the fruit fly, Drosophila melanogaster, has a major size-control function in animals. All key components of the Hippo pathway, including the transcriptional coactivator Yorkie that is the most critical substrate and downstream effector of the Hippo kinase cassette, are found in the silkworm, Bombyx mori. As revealed by microarray and quantitative real-time PCR, expression of Hippo pathway genes is particularly enriched in several mitotic tissues, including the ovary, testis, and wing disc...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27447797/novel-infection-system-of-recombinant-bmbdv-dna-into-bmn-cells-of-silkworm-bombyx-mori
#15
Rui Guo, Guangli Cao, Yuexiong Zhu, Dhiraj Kumar, Renyu Xue, Yahong Lu, Xiaolong Hu, Chengliang Gong
Bombyx mori bidensovirus (BmBDV) was previously termed as Bombyx mori densovirus type 2 and later it was reclassified in the new genus bidensovirus of the new family Bidnaviridae. The genome of BmBDV Zhenjiang isolate (BmBDV-Z) consists of two non-homologous single-stranded linear DNA molecules VD1 and VD2 which are encapsidated into separate virion. To investigate the infectivity of BmBDV DNA, recombinant plasmids pGEM-VD1 inserted with VD1 genome were transfected into the BmN cells of silkworm. Structural proteins of BmBDV were detected with Western blot and immunofluorescence assay, which indicates pGEM-VD1 replicated in the transfected BmN cells and viral proteins were also expressed...
October 2016: Current Microbiology
https://www.readbyqxmd.com/read/27414795/a-dna-binding-protein-is-required-for-viral-replication-and-transcription-in-bombyx-mori-nucleopolyhedrovirus
#16
Cui Zhao, Chen Zhang, Bin Chen, Yanghui Shi, Yanping Quan, Zuoming Nie, Yaozhou Zhang, Wei Yu
A DNA-binding protein (DBP) [GenBank accession number: M63416] of Bombyx mori nuclear polyhedrosis virus (BmNPV) has been reported to be a regulatory factor in BmNPV, but its detailed functions remain unknown. In order to study the regulatory mechanism of DBP on viral proliferation, genome replication, and gene transcription, a BmNPV dbp gene knockout virus dbp-ko-Bacmid was generated by the means of Red recombination system. In addition, dbp-repaired virus dbp-re-Bacmid was constructed by the means of the Bac to Bac system...
2016: PloS One
https://www.readbyqxmd.com/read/27245143/screening-and-optimization-of-an-efficient-bombyx-mori-nucleopolyhedrovirus-inducible-promoter
#17
Ming-Ya Cao, Xiu-Xiu Kuang, Hai-Qing Li, Xue-Jiao Lei, Wen-Fu Xiao, Zhan-Qi Dong, Jun Zhang, Nan Hu, Ting-Ting Chen, Cheng Lu, Min-Hui Pan
Pathogen-inducible promoters have been studied extensively and widely used in resistance breeding and gene therapy. However, few reports have been published that explore the efficacy of Bombyx mori nucleopolyhedrovirus (BmNPV)-inducible promoters in antiviral research in the Bombyx mori (Lepidoptera). Here, we screened BmNPV promoters (VP1054, P33, Bm21, Bm122, 39K, P143, and P6.9) and found that the 39K promoter had the highest BmNPV-induced transcriptional activity by dual-luciferase reporter assays system...
August 10, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27244571/a-role-for-the-anti-viral-host-defense-mechanism-in-the-phylogenetic-divergence-in-baculovirus-evolution
#18
Toshihiro Nagamine, Yasushi Sako
Although phylogenic analysis often suggests co-evolutionary relationships between viruses and host organisms, few examples have been reported at the microevolutionary level. Here, we show a possible example in which a species-specific anti-viral response may drive phylogenic divergence in insect virus evolution. Two baculoviruses, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV), have a high degree of DNA sequence similarity, but exhibit non-overlapping host specificity...
2016: PloS One
https://www.readbyqxmd.com/read/27142667/characterization-of-the-viral-fibroblast-growth-factor-homolog-of-helicoverpa-armigera-single-nucleopolyhedrovirus
#19
Feifei Yin, Ruikun Du, Wenhua Kuang, Guang Yang, Hualin Wang, Fei Deng, Zhihong Hu, Manli Wang
Fibroblast growth factor (FGF) is found throughout multicellular organisms; however, fgf homologs (vfgf) have only been identified among viruses in lepidopteran baculoviruses. The function of vFGFs from Group I alphabaculoviruses, including Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV), involves accelerated killing of infected larvae by both viruses. The vFGF of Group II alphabaculovirus is structurally different from that of Group I alphabaculovirus, with a larger C-terminal region and additional N-linked glycosylation sites...
June 2016: Virologica Sinica
https://www.readbyqxmd.com/read/27059494/expression-and-characterization-of-recombinant-serratia-liquefaciens-nucleases-produced-with-baculovirus-mediated-silkworm-expression-system
#20
Kazuhiro Iiyama, Jae Man Lee, Tuneyuki Tatsuke, Hiroaki Mon, Takahiro Kusakabe
Baculovirus-Bombyx mori protein expression system has mainly been used for translation of eukaryotic proteins. In contrast, information pertaining to bacterial protein expression using this system is not sufficient. Therefore, recombinant nucleases from Serratia liquefaciens (rSlNucAs) were expressed in a Baculovirus-B. mori protein expression system. rSlNucAs containing the native signal peptide (rSlNucA-NSP) or silkworm 30-K signal peptide (rSlNucA-30K) at the NH2-terminus were constructed to enable secretion into the extracellular fraction...
June 2016: Molecular Biotechnology
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