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Tchapyjnikov

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https://www.readbyqxmd.com/read/26806548/response-to-immunotherapy-in-a-patient-with-landau-kleffner-syndrome-and-grin2a-mutation
#1
Nina Fainberg, Amy Harper, Dmitry Tchapyjnikov, Mohamad A Mikati
Landau-Kleffner syndrome (LKS) has been demonstrated in the past to respond to immunotherapy. Recently, some cases of LKS have been shown to be secondary to glutamate receptor (GRIN2A) mutations. Whether such cases respond to immunotherapy is not known. Here, we present the case of a 3-year-old boy with LKS found to have a GRIN2A heterozygous missense mutation, whose clinical symptoms and EEG responded to a course of combination oral steroids and monthly infusions of intravenous immunoglobulin. He then relapsed after discontinuation of this therapy, and responded again after a second course of intravenous immunoglobulin...
March 1, 2016: Epileptic Disorders: International Epilepsy Journal with Videotape
https://www.readbyqxmd.com/read/19996160/proteomic-profiling-of-nuclei-from-native-renal-inner-medullary-collecting-duct-cells-using-lc-ms-ms
#2
Dmitry Tchapyjnikov, Yuedan Li, Trairak Pisitkun, Jason D Hoffert, Ming-Jiun Yu, Mark A Knepper
Vasopressin is a peptide hormone that regulates renal water excretion in part through its actions on the collecting duct. The regulation occurs in part via control of transcription of genes coding for the water channels aquaporin-2 (Aqp2) and aquaporin-3 (Aqp3). To identify transcription factors expressed in collecting duct cells, we have carried out LC-MS/MS-based proteomic profiling of nuclei isolated from native rat inner medullary collecting ducts (IMCDs). To maximize the number of proteins identified, we matched spectra to rat amino acid sequences using three different search algorithms (SEQUEST, InsPecT, and OMSSA)...
February 4, 2010: Physiological Genomics
https://www.readbyqxmd.com/read/19056867/large-scale-proteomics-and-phosphoproteomics-of-urinary-exosomes
#3
Patricia A Gonzales, Trairak Pisitkun, Jason D Hoffert, Dmitry Tchapyjnikov, Robert A Star, Robert Kleta, Nam Sun Wang, Mark A Knepper
Normal human urine contains large numbers of exosomes, which are 40- to 100-nm vesicles that originate as the internal vesicles in multivesicular bodies from every renal epithelial cell type facing the urinary space. Here, we used LC-MS/MS to profile the proteome of human urinary exosomes. Overall, the analysis identified 1132 proteins unambiguously, including 177 that are represented on the Online Mendelian Inheritance in Man database of disease-related genes, suggesting that exosome analysis is a potential approach to discover urinary biomarkers...
February 2009: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/18606813/vasopressin-stimulated-increase-in-phosphorylation-at-ser269-potentiates-plasma-membrane-retention-of-aquaporin-2
#4
Jason D Hoffert, Robert A Fenton, Hanne B Moeller, Brigitte Simons, Dmitry Tchapyjnikov, Bradley W McDill, Ming-Jiun Yu, Trairak Pisitkun, Feng Chen, Mark A Knepper
Vasopressin controls water excretion through regulation of aquaporin-2 (AQP2) trafficking in renal collecting duct cells. Using mass spectrometry, we previously demonstrated four phosphorylated serines (Ser256, Ser261, Ser264, and Ser269) in the carboxyl-terminal tail of rat AQP2. Here, we used phospho-specific antibodies and protein mass spectrometry to investigate the roles of vasopressin and cyclic AMP in the regulation of phosphorylation at Ser269 and addressed the role of this site in AQP2 trafficking...
September 5, 2008: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/18596208/large-scale-quantitative-lc-ms-ms-analysis-of-detergent-resistant-membrane-proteins-from-rat-renal-collecting-duct
#5
Ming-Jiun Yu, Trairak Pisitkun, Guanghui Wang, Juan F Aranda, Patricia A Gonzales, Dmitry Tchapyjnikov, Rong-Fong Shen, Miguel A Alonso, Mark A Knepper
In the renal collecting duct, vasopressin controls transport of water and solutes via regulation of membrane transporters such as aquaporin-2 (AQP2) and the epithelial urea transporter UT-A. To discover proteins potentially involved in vasopressin action in rat kidney collecting ducts, we enriched membrane "raft" proteins by harvesting detergent-resistant membranes (DRMs) of the inner medullary collecting duct (IMCD) cells. Proteins were identified and quantified with LC-MS/MS. A total of 814 proteins were identified in the DRM fractions...
September 2008: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/17686955/forskolin-stimulates-phosphorylation-and-membrane-accumulation-of-ut-a3
#6
Mitsi A Blount, Janet D Klein, Christopher F Martin, Dmitry Tchapyjnikov, Jeff M Sands
UT-A1 is regulated by vasopressin and is localized to the apical membrane and intracellular compartment of inner medullary collecting duct (IMCD) cells. UT-A3 is also expressed in the IMCD and is regulated by forskolin in heterologous systems. The goal of the present study is to investigate mechanisms by which vasopressin regulates UT-A3 in rat IMCD. In fresh suspensions of rat IMCD, forskolin increases the phosphorylation of UT-A3, similar to UT-A1. Biotinylation studies indicate that UT-A3 is located in the plasma membrane...
October 2007: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/16988959/comparison-of-germanium-oxide-fibers-with-silica-and-sapphire-fiber-tips-for-transmission-of-erbium-yag-laser-radiation
#7
COMPARATIVE STUDY
Travis J Polletto, Anthony K Ngo, Alexei Tchapyjnikov, Ken Levin, Danh Tran, Nathaniel M Fried
BACKGROUND AND OBJECTIVES: Endoscopic applications of the Erbium:YAG laser have been limited due to the lack of a suitable optical fiber delivery system. The purpose of this study was to compare the transmission of Er:YAG laser radiation through germanium oxide trunk fibers with silica and sapphire fiber tips for potential use in contact tissue ablation during endoscopy. STUDY DESIGN/MATERIALS AND METHODS: Er:YAG laser radiation with a wavelength of 2.94 microm, pulse length of 300 microseconds, pulse energies from 5 to 1,360 mJ, coupled into pulse repetition rates of 3-10 Hz, was through 1-m-long germanium oxide fibers with either 1-cm-long, 550-microm-diameter silica or sapphire tips...
September 2006: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/16449382/high-throughput-identification-of-imcd-proteins-using-lc-ms-ms
#8
COMPARATIVE STUDY
Trairak Pisitkun, Jared Bieniek, Dmitry Tchapyjnikov, Guanghui Wang, Wells W Wu, Rong-Fong Shen, Mark A Knepper
The inner medullary collecting duct (IMCD) is an important site of vasopressin-regulated water and urea transport. Here we have used protein mass spectrometry to investigate the proteome of the IMCD cell and how it is altered in response to long-term vasopressin administration in rats. IMCDs were isolated from inner medullas of rats, and IMCD proteins were identified by liquid chromatography/tandem mass spectrometry (LC-MS/MS). We present a WWW-based "IMCD Proteome Database" containing all IMCD proteins identified in this study (n = 704) and prior MS-based identification studies (n = 301)...
April 13, 2006: Physiological Genomics
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