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Biofilm ear

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https://www.readbyqxmd.com/read/28798773/planktonic-growth-of-pseudomonas-aeruginosa-around-a-dual-species-biofilm-supports-the-growth-of-fusobacterium-nucleatum-within-that-biofilm
#1
James C Wang, Joehassin Cordero, Yan Sun, Mayank Aranke, Randall Wolcott, Jane A Colmer-Hamood, Abdul N Hamood
PURPOSE: The goal of this study was to understand the potential interaction between Pseudomonas aeruginosa and Fusobacterium nucleatum within the middle ear. METHODS: We examined the microbiota of ear fluid and tympanostomy tubes (TTs) obtained from patients with posttympanostomy tube otorrhea. We also examined biofilms formed by P. aeruginosa and F. nucleatum, singly or together, under aerobic or anaerobic conditions. RESULTS: While the facultative anaerobe P...
2017: International Journal of Otolaryngology
https://www.readbyqxmd.com/read/28796849/neutrophil-extracellular-traps-and-fibrin-in-otitis-media-analysis-of-human-and-chinchilla-temporal-bones
#2
Patricia A Schachern, Geeyoun Kwon, David E Briles, Patricia Ferrieri, Steven Juhn, Sebahattin Cureoglu, Michael M Paparella, Vladimir Tsuprun
Background: Bacterial resistance in acute otitis can result in bacterial persistence and biofilm formation, triggering chronic and recurrent infections. Objective: To investigate the middle ear inflammatory response to bacterial infection in human and chinchilla temporal bones. Design, Setting, and Participants: Six chinchillas underwent intrabullar inoculations with 0.5 mL of 106 colony-forming units (CFUs) of Streptococcus pneumoniae, serotype 2...
August 10, 2017: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28736916/hyaluronate-effect-on-bacterial-biofilm-in-ent-district-infections-a-review
#3
REVIEW
Alberto Vito Marcuzzo, Margherita Tofanelli, Francesca Boscolo Nata, Annalisa Gatto, Giancarlo Tirelli
Bacterial resistance is a growing phenomenon which led the scientific community to search for new therapeutic targets, such as biofilm. A bacterial biofilm is a surface-associated agglomerate of microorganisms embedded in a self-produced extracellular polymeric matrix made of polysaccharides, nucleic acids, and proteins. Scientific literature offers several reports on a biofilm's role in infections regarding various body districts. The presence of a bacterial biofilm is responsible for poor efficacy of antibiotic therapies along with bacterial infections in ear, nose, and throat (ENT) districts such as the oral cavity, ear, nasal cavities, and nasal sinuses...
September 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28653118/quantitative-pneumatic-otoscopy-using-a-light-based-ranging-technique
#4
Ryan L Shelton, Ryan M Nolan, Guillermo L Monroy, Paritosh Pande, Michael A Novak, Ryan G Porter, Stephen A Boppart
Otitis media is the leading cause of hearing loss in children. It is commonly associated with fluid in the ear, which can result in up to 45 dB of hearing loss for extended periods of time during a child's most important developmental years. Accurate assessment of middle ear effusions is an important part of understanding otitis media. Current technologies used to diagnose otitis media with effusion are pneumatic otoscopy, tympanometry, and acoustic reflectometry. While all of these techniques can reasonably diagnose the presence of an effusion, they provide limited information about the infection present behind the tympanic membrane...
August 2017: Journal of the Association for Research in Otolaryngology: JARO
https://www.readbyqxmd.com/read/28647025/olumacostat-glasaretil-a-promising-topical-sebum-suppressing-agent-that-affects-all-major-pathogenic-factors-of-acne-vulgaris
#5
Bodo C Melnik
Hunt et al. show that olumacostat glasaretil, an inhibitor of acetyl coenzyme A carboxylase, reduces saturated and monounsaturated fatty acyl chains in sebaceous lipids. Topical olumacostat glasaretil application decreases hamster ear sebaceous gland size and shows efficacy in treating patients with acne vulgaris. Olumacostat glasaretil-mediated sebum suppression may reduce Propionibacterium acnes growth and biofilm formation, comedogenesis, and inflammation.
July 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28557053/a-novel-streptococcal-cell-cell-communication-peptide-promotes-pneumococcal-virulence-and-biofilm-formation
#6
Rolando A Cuevas, Rory Eutsey, Anagha Kadam, Jacob A West-Roberts, Carol A Woolford, Aaron P Mitchell, Kevin M Mason, N Luisa Hiller
Streptococcus pneumoniae (pneumococcus) is a major human pathogen. It is a common colonizer of the human respiratory track, where it utilizes cell-cell communication systems to coordinate population-level behaviors. We reasoned that secreted peptides that are highly expressed during infection are pivotal for virulence. Thus, we used in silico pattern searches to define a pneumococcal secretome and analyzed the transcriptome of the clinically important PMEN1 lineage to identify which peptide-encoding genes are highly expressed in vivo...
August 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28466356/recurrent-otorrhea-in-chronic-suppurative-otitis-media-is-biofilm-the-missing-link
#7
Ramon Gordon Jensen, Helle Krogh Johansen, Thomas Bjarnsholt, Steffen Robert Eickhardt-Sørensen, Preben Homøe
Dispersal of bacteria from a biofilm in the middle ear, serving as a bacterial reservoir, could explain the recurrent and chronic nature of chronic suppurative otitis media (CSOM). The objective of this study is to investigate if the same strains of bacteria could be detected in repeated episodes of otorrhea in CSOM. In a prospective case series at a primary healthcare clinic in Nuuk, Greenland, patients with more than 14 days of otorrhea were included consecutively. Samples for culturing and biofilm analysis were taken at enrollment and at any subsequent episode with otorrhea...
May 2, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28459043/in-vitro-multi-species-biofilms-of-methicillin-resistant-staphylococcus-aureus-and-pseudomonas-aeruginosa-and-their-host-interaction-during-in-vivo-colonization-of-an-otitis-media-rat-model
#8
Mukesh K Yadav, Sung-Won Chae, Yoon Young Go, Gi Jung Im, Jae-Jun Song
Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each-other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities in vitro and their interaction with the host during in vivo colonization using an OM rat-model...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28381402/transcutaneous-immunization-with-a-band-aid-prevents-experimental-otitis-media-in-a-polymicrobial-model
#9
Laura A Novotny, John D Clements, Steven D Goodman, Lauren O Bakaletz
Otitis media (OM) is a common pediatric disease, and nontypeable Haemophilus influenzae (NTHI) is the predominant pathogen in chronic OM, recurrent OM, and OM associated with treatment failure. OM is also a polymicrobial disease, wherein an upper respiratory tract viral infection predisposes to ascension of NTHI from the nasopharynx, the site of colonization, to the normally sterile middle ear, resulting in disease. Using a clinically relevant viral-bacterial coinfection model of NTHI-induced OM, we performed transcutaneous immunization (TCI) via a band-aid delivery system to administer each of three promising NTHI vaccine candidates derived from bacterial adhesive proteins and biofilm mediators: recombinant soluble PilA (rsPilA), chimV4, and integration host factor...
June 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28335605/opportunistic-sampling-of-roadkill-as-an-entry-point-to-accessing-natural-products-assembled-by-bacteria-associated-with-non-anthropoidal-mammalian-microbiomes
#10
Jeremy L Motley, Blake W Stamps, Carter A Mitchell, Alec T Thompson, Jayson Cross, Jianlan You, Douglas R Powell, Bradley S Stevenson, Robert H Cichewicz
Few secondary metabolites have been reported from mammalian microbiome bacteria despite the large numbers of diverse taxa that inhabit warm-blooded higher vertebrates. As a means to investigate natural products from these microorganisms, an opportunistic sampling protocol was developed, which focused on exploring bacteria isolated from roadkill mammals. This initiative was made possible through the establishment of a newly created discovery pipeline, which couples laser ablation electrospray ionization mass spectrometry (LAESIMS) with bioassay testing, to target biologically active metabolites from microbiome-associated bacteria...
March 24, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28096183/pneumococcal-neuraminidase-a-nana-promotes-biofilm-formation-and-synergizes-with-influenza-a-virus-in-nasal-colonization-and-middle-ear-infection
#11
John T Wren, Lance K Blevins, Bing Pang, Ankita Basu Roy, Melissa B Oliver, Jennifer L Reimche, Jessie E Wozniak, Martha A Alexander-Miller, W Edward Swords
Even in the vaccine era, Streptococcus pneumoniae (the pneumococcus) remains a leading cause of otitis media, a significant public health burden, in large part because of the high prevalence of nasal colonization with the pneumococcus in children. The primary pneumococcal neuraminidase, NanA, which is a sialidase that catalyzes the cleavage of terminal sialic acids from host glycoconjugates, is involved in both of these processes. Coinfection with influenza A virus, which also expresses a neuraminidase, exacerbates nasal colonization and disease by S...
April 2017: Infection and Immunity
https://www.readbyqxmd.com/read/27956464/resistance-of-non-typeable-haemophilus-influenzae-biofilms-is-independent-of-biofilm-size
#12
Jennifer L Reimche, Daniel J Kirse, Amy S Whigham, W Edward Swords
The inflammatory middle ear disease known as otitis media can become chronic or recurrent in some cases due to failure of the antibiotic treatment to clear the bacterial etiological agent. Biofilms are known culprits of antibiotic-resistant infections; however, the mechanisms of resistance for non-typeable Haemophilus influenzae biofilms have not been completely elucidated. In this study, we utilized in vitro static biofilm assays to characterize clinical strain biofilms and addressed the hypothesis that biofilms with greater biomass and/or thickness would be more resistant to antimicrobial-mediated eradication than thinner and/or lower biomass biofilms...
February 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/27919900/n-acetyl-l-cysteine-and-cysteamine-as-new-strategies-against-mixed-biofilms-of-nonencapsulated-streptococcus-pneumoniae-and-nontypeable-haemophilus-influenzae
#13
Mirian Domenech, Ernesto García
Acute otitis media, a polymicrobial disease of the middle ear cavity of children, is a significant public health problem worldwide. It is most frequently caused by encapsulated Streptococcus pneumoniae and nontypeable Haemophilus influenzae, although the widespread use of pneumococcal conjugate vaccines is apparently producing an increase in the carriage of nonencapsulated S. pneumoniae Frequently, pneumococci and H. influenzae live together in the human nasopharynx, forming a self-produced biofilm. Biofilms present a global medical challenge since the inherent antibiotic resistance of their producers demands the use of large doses of antibiotics over prolonged periods...
February 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27792773/rna-seq-transcriptomic-responses-of-full-thickness-dermal-excision-wounds-to-pseudomonas-aeruginosa-acute-and-biofilm-infection
#14
S L Rajasekhar Karna, Peter D'Arpa, Tsute Chen, Li-Wu Qian, Andrea B Fourcaudot, Kazuyoshi Yamane, Ping Chen, Johnathan J Abercrombie, Tao You, Kai P Leung
Pseudomonas aeruginosa infections of wounds in clinical settings are major complications whose outcomes are influenced by host responses that are not completely understood. Herein we evaluated transcriptomic changes of wounds as they counter P. aeruginosa infection-first active infection, and then chronic biofilm infection. We used the dermal full-thickness, rabbit ear excisional wound model. We studied the wound response: towards acute infection at 2, 6, and 24 hrs after inoculating 106 bacteria into day-3 wounds; and, towards more chronic biofilm infection of wounds similarly infected for 24 hrs but then treated with topical antibiotic to coerce biofilm growth and evaluated at day 5 and 9 post-infection...
2016: PloS One
https://www.readbyqxmd.com/read/27755287/biofilm-formation-on-bandage-contact-lenses-worn-by-patients-with-the-boston-type-1-keratoprosthesis-a-pilot-comparison-study-of-prophylactic-topical-vancomycin-15-mg-ml-and-linezolid-0-2
#15
Asim V Farooq, Joshua H Hou, Sarmad Jassim, Zeeshan Haq, Elmer Y Tu, Jose de la Cruz, Maria S Cortina
OBJECTIVES: To determine the rate of biofilm formation on bandage contact lenses worn by patients with the Boston type 1 keratoprosthesis (K-Pro) while on prophylactic topical vancomycin versus linezolid. METHODS: Patients wearing a bandage contact lens (BCL) with a K-Pro were eligible for enrollment. After irrigation of the ocular surface with 5% povidone-iodine solution, each patient was placed on either topical vancomycin 15 mg/mL or linezolid 0.2% BID for one month...
October 13, 2016: Eye & Contact Lens
https://www.readbyqxmd.com/read/27754780/in-vitro-antimicrobial-efficacy-of-tobramycin-against-staphylococcus-aureus-biofilms-in-combination-with-or-without-dnase-i-and-or-dispersin-b-a-preliminary-investigation
#16
Charlene Babra Waryah, Kelsi Wells, Dulantha Ulluwishewa, Nigel Chen-Tan, Jully Gogoi-Tiwari, Joshua Ravensdale, Paul Costantino, Anke Gökçen, Andreas Vilcinskas, Jochen Wiesner, Trilochan Mukkur
Staphylococcus aureus in biofilms is highly resistant to the treatment with antibiotics, to which the planktonic cells are susceptible. This is likely to be due to the biofilm creating a protective barrier that prevents antibiotics from accessing the live pathogens buried in the biofilm. S. aureus biofilms consist of an extracellular matrix comprising, but not limited to, extracellular bacterial DNA (eDNA) and poly-β-1, 6-N-acetyl-d-glucosamine (PNAG). Our study revealed that despite inferiority of dispersin B (an enzyme that degrades PNAG) to DNase I that cleaves eDNA, in dispersing the biofilm of S...
April 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27630633/-get-in-early-biofilm-and-wax-moth-galleria-mellonella-models-reveal-new-insights-into-the-therapeutic-potential-of-clostridium-difficile-bacteriophages
#17
Janet Y Nale, Mahananda Chutia, Philippa Carr, Peter T Hickenbotham, Martha R J Clokie
Clostridium difficile infection (CDI) is a global health threat associated with high rates of morbidity and mortality. Conventional antibiotic CDI therapy can result in treatment failure and recurrent infection. C. difficile produces biofilms which contribute to its virulence and impair antimicrobial activity. Some bacteriophages (phages) can penetrate biofilms and thus could be developed to either replace or supplement antibiotics. Here, we determined the impact of a previously optimized 4-phage cocktail on C...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27486827/multitask-imidazolium-salt-additives-for-innovative-poly-l-lactide-biomaterials-morphology-control-candida-spp-biofilm-inhibition-human-mesenchymal-stem-cell-biocompatibility-and-skin-tolerance
#18
Clarissa M L Schrekker, Yuri C A Sokolovicz, Maria G Raucci, Balaji S Selukar, Joice S Klitzke, William Lopes, Claudio A M Leal, Igor O P de Souza, Griselda B Galland, João Henrique Z Dos Santos, Raquel S Mauler, Moshe Kol, Samuel Dagorne, Luigi Ambrosio, Mário L Teixeira, Jonder Morais, Richard Landers, Alexandre M Fuentefria, Henri S Schrekker
Candida species have great ability to colonize and form biofilms on medical devices, causing infections in human hosts. In this study, poly(l-lactide) films with different imidazolium salt (1-n-hexadecyl-3-methylimidazolium chloride (C16MImCl) and 1-n-hexadecyl-3-methylimidazolium methanesulfonate (C16MImMeS)) contents were prepared, using the solvent casting process. Poly(l-lactide)-imidazolium salt films were obtained with different surface morphologies (spherical and directional), and the presence of the imidazolium salt in the surface was confirmed...
August 24, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27234665/role-of-bacterial-biofilm-in-development-of-middle-ear-effusion
#19
COMPARATIVE STUDY
Sedeek Abd El-Salam Tawfik, Ahmed Aly Ibrahim, Iman Mamdoh Talaat, Soliman Samy Abd El-Raouf El-Alkamy, Ahmed Youssef
Biofilms have been implicated in the development of several chronic upper respiratory tract infections. Role of bacterial biofilms has been well studied in the pathogenesis of chronic rhinosinusitis. However, its impact on development of middle ear effusion is still a matter of debate. To study the extent of surface adenoid biofilm and evaluate its role in the pathogenesis of chronic otitis media with effusion in children. The study was carried out on 40 children in Alexandria Main University Hospital between 1 and 16 years of age without sex predilection, who were divided into two groups...
November 2016: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/27099692/impact-of-plant-extracts-and-antibiotics-on-biofilm-formation-of-clinical-isolates-from-otitis-media
#20
Saba Rehman, Shahbaz Mujtaba Ghauri, Anjum Nasim Sabri
BACKGROUND: Otitis media can lead to severe health consequences, and is the most common reason for antibiotic prescriptions and biofilm-mediated infections. However, the increased pattern of drug resistance in biofilm forming bacteria complicates the treatment of such infections. OBJECTIVES: This study was aimed to estimate the biofilm formation potential of the clinical isolates of otitis media, and to evaluate the efficacy of antibiotics and plant extracts as alternative therapeutic agents in biofilm eradication...
January 2016: Jundishapur Journal of Microbiology
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