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https://www.readbyqxmd.com/read/28039100/utility-of-systematic-isolation-of-immune-cell-subsets-from-hiv-infected-individuals-for-mirna-profiling
#1
Manel E Bargalló, Alberto C Guardo, Maria J Maleno, Laia Miralles, Lander Egaña-Gorroño, Tuixent Escribà, Felipe García, Jose M Gatell, Mireia Arnedo, Montserrat Plana
INTRODUCTION: Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune cell subsets by immunomagnetic separation and the RNA integrity after isolated cells from samples of HIV-infected patients. METHODS: PBMC from Healthy Controls (HC, n=15), Elite Controllers (EC, n=15), Viremic Controllers (VC, n=15), Viremic Progressors (VP, n=15) and HIV-infected patients on therapy (ART, n=15) were isolated by Ficoll-Paque density gradient centrifugation...
December 27, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28005686/transcriptome-analyses-identify-key-cellular-factors-associated-with-hiv-1-associated-neuropathogenesis-in-infected-men
#2
Narasimhan J Venkatachari, Siddhartha Jain, Leah Walker, Shalmali Bivalkar-Mehla, Ansuman Chattopadhyay, Ziv Bar-Joseph, Charles Rinaldo, Ann Ragin, Eric Seaberg, Andrew Levine, James Becker, Eileen Martin, Ned Sacktor, Velpandi Ayyavoo
OBJECTIVE: HIV-1 viral proteins and host inflammatory factors have a direct role in neuronal toxicity in in vitro, however, the contribution of these factors in vivo in HIV-1 associated neurocognitive disorder (HAND) is not fully understood. We applied novel Systems Biology approaches to identify specific cellular and viral factors and their related pathways that are associated with different stages of HAND. DESIGN: A cross-sectional study of individuals enrolled in the Multicenter AIDS Cohort Study (MACS) including HIV-1 seronegative (N = 36) and HIV-1 seropositive individuals without neurocognitive symptoms (N = 16), or with mild neurocognitive disorder (MND) (N = 8) or HIV-associated dementia (HAD) (N = 16)...
December 21, 2016: AIDS
https://www.readbyqxmd.com/read/28003477/argonaute-proteins-regulate-hiv-1-multiply-spliced-rna-and-viral-production-in-a-dicer-independent-manner
#3
Agathe Eckenfelder, Emmanuel Ségéral, Natalia Pinzón, Damien Ulveling, Céline Amadori, Marine Charpentier, Sabine Nidelet, Jean-Paul Concordet, Jean-François Zagury, Jean-Christophe Paillart, Clarisse Berlioz-Torrent, Hervé Seitz, Stéphane Emiliani, Sarah Gallois-Montbrun
Argonaute (Ago) proteins associate with microRNAs (miRNAs) to form the core of the RNA-induced silencing complex (RISC) that mediates post-transcriptional gene silencing of target mRNAs. As key players in anti-viral defense, Ago proteins are thought to have the ability to interact with human immunodeficiency virus type 1 (HIV-1) RNA. However, the role of this interaction in regulating HIV-1 replication has been debated. Here, we used high throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP) to explore the interaction between Ago2 and HIV-1 RNA in infected cells...
December 20, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27993935/mirna-profiling-of-human-naive-cd4-t-cells-links-mir-34c-5p-to-cell-activation-and-hiv-replication
#4
Andreia J Amaral, Jorge Andrade, Russell B Foxall, Paula Matoso, Ana M Matos, Rui S Soares, Cheila Rocha, Christian G Ramos, Rita Tendeiro, Ana Serra-Caetano, José A Guerra-Assunção, Mariana Santa-Marta, João Gonçalves, Margarida Gama-Carvalho, Ana E Sousa
Cell activation is a vital step for T-cell memory/effector differentiation as well as for productive HIV infection. To identify novel regulators of this process, we used next-generation sequencing to profile changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stimulation and/or HIV infection. Our results demonstrate, for the first time, the transcriptional up-regulation of miR-34c-5p in response to TCR stimulation in naive CD4 T cells. The induction of this miR was further consistently found to be reduced by both HIV-1 and HIV-2 infections...
December 19, 2016: EMBO Journal
https://www.readbyqxmd.com/read/27919232/microrna-27a-rs895819-is-associated-with-obesity-in-hiv-infected-preeclamptic-black-south-african-women-on-haart
#5
Niren Ray Maharaj, Prithiksha Ramkaran, Siddharthiya Pillay, Anil Amichund Chuturgoon
BACKGROUND: Preeclampsia (PE) and HIV/AIDS present a major health challenge globally. South Africa has the highest disease burden of both HIV/AIDS and PE in the world. Despite extensive research, the pathophysiology of these conditions is not completely understood, however a genetic predisposition in women may affect susceptibility. MiRNA-27a regulates adipogenesis and glucose metabolism. A single nucleotide polymorphism (SNP) in miRNA-27a (rs895819T > C) has shown to have disparate effects in various populations...
December 5, 2016: BMC Medical Genetics
https://www.readbyqxmd.com/read/27886048/interactions-between-the-hiv-1-unspliced-mrna-and-host-mrna-decay-machineries
#6
REVIEW
Daniela Toro-Ascuy, Bárbara Rojas-Araya, Fernando Valiente-Echeverría, Ricardo Soto-Rifo
The human immunodeficiency virus type-1 (HIV-1) unspliced transcript is used both as mRNA for the synthesis of structural proteins and as the packaged genome. Given the presence of retained introns and instability AU-rich sequences, this viral transcript is normally retained and degraded in the nucleus of host cells unless the viral protein REV is present. As such, the stability of the HIV-1 unspliced mRNA must be particularly controlled in the nucleus and the cytoplasm in order to ensure proper levels of this viral mRNA for translation and viral particle formation...
November 23, 2016: Viruses
https://www.readbyqxmd.com/read/27882942/emerging-roles-of-extracellular-vesicles-in-neurodegenerative-disorders-focus-on-hiv-associated-neurological-complications
#7
REVIEW
Guoku Hu, Lu Yang, Yu Cai, Fang Niu, Frank Mezzacappa, Shannon Callen, Howard S Fox, Shilpa Buch
Exosomes are membrane-enriched extracellular vesicles with a proposed diameter in the range of 30-100 nm. They are released during both normal homeostasis as well as under pathological conditions by most cell types. In recent years, there has been robust interest in the study of these vesicles as conduits for the delivery of information between cells in both analogous as well as disparate tissues. Their ability to transport specialized cargo including signaling mediators, proteins, messenger RNA and miRNAs characterizes these vesicles as primary facilitators of cell-to-cell communication and regulation...
November 24, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27852859/kshv-micrornas-target-gadd45b-to-protect-infected-cells-from-cell-cycle-arrest-and-apoptosis
#8
Xiaoyan Liu, Christine Happel, Joseph M Ziegelbauer
: Kaposi's sarcoma is one of the most common malignancies in HIV-infected individuals. The responsible agent, Kaposi's sarcoma-associated herpesvirus (KSHV, HHV8) expresses multiple microRNAs (miRNAs), but the targets and functions of these miRNAs are not completely understood. After infection in primary endothelial cells with KSHV, growth arrest DNA damage-inducible gene 45 beta (GADD45B) is one of the most repressed genes using genomic expression profiling. GADD45B was also repressed in mRNA expression profiling experiments when KSHV miRNAs were introduced to uninfected cells...
November 16, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27761954/novel-insights-into-role-of-mir-320a-vdac1-axis-in-astrocyte-mediated-neuronal-damage-in-neuroaids
#9
Mahar Fatima, Bharat Prajapati, Kanza Saleem, Rina Kumari, Chitra Mohindar Singh Singal, Pankaj Seth
Astroglia are indispensable component of the tripartite synapse ensheathing innumerous soma and synapses. Its proximity to neurons aids the regulation of neuronal functions, health and survival through dynamic neuroglia crosstalk. Susceptibility of astrocyte to HIV-1 infection and subsequent latency culminates in compromised neuronal health. The viral protein HIV-1 transactivator of transcription (Tat) is neurotoxic. HIV-1 Tat is detected in brain of AIDS patients even in cases where viral load is non-detectable due to successful HAART therapy...
February 2017: Glia
https://www.readbyqxmd.com/read/27749601/microrna-210-microrna-331-and-microrna-7-are-differentially-regulated-in-treated-hiv-1-infected-individuals-and-are-associated-with-markers-of-systemic-inflammation
#10
Vibe Ballegaard, Ulrik Ralfkiaer, Karin K Pedersen, Malene Hove, Simon Koplev, Peter Brændstrup, Lars P Ryder, Hans O Madsen, Jan Gerstoft, Kirsten Grønbæk, Susanne D Nielsen
OBJECTIVE: Inflammation may contribute to increased risk of cardiovascular disease (CVD) in HIV-1 infection. MicroRNAs (miRNAs) are involved in the regulation of inflammation. In treated HIV-1-infected individuals, we aimed to identify differentially expressed miRNAs with known roles in inflammation and CVD risk, and to investigate associations between these and systemic inflammation. METHODS: In a screening cohort including 14 HIV-1-infected individuals and nine uninfected controls microarray profiling was performed using peripheral blood mononuclear cells (PBMC)...
October 3, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/27701053/microrna-related-polymorphisms-and-non-hodgkin-lymphoma-susceptibility-in-the-multicenter-aids-cohort-study
#11
Erin C Peckham-Gregory, Dharma R Thapa, Jeremy Martinson, Priya Duggal, Sudhir Penugonda, Jay H Bream, Po-Yin Chang, Sugandha Dandekar, Shen-Chih Chang, Roger Detels, Otoniel Martínez-Maza, Zuo-Feng Zhang, Shehnaz K Hussain
BACKGROUND: MicroRNAs, small non-coding RNAs involved in gene regulation, are implicated in lymphomagenesis. We evaluated whether genetic variations in microRNA coding regions, binding sites, or biogenesis genes (collectively referred to as miRNA-SNPs) were associated with risk of AIDS-associated non-Hodgkin lymphoma (AIDS-NHL), and serum levels of four lymphoma-related microRNAs. METHODS: Twenty-five miRNA-SNPs were genotyped in 180 AIDS-NHL cases and 529 HIV-infected matched controls from the Multicenter AIDS Cohort Study (MACS), and real-time polymerase chain reaction was used to quantify serum microRNA levels...
December 2016: Cancer Epidemiology
https://www.readbyqxmd.com/read/27634380/hiv-1-tat-shortened-neurite-outgrowth-through-regulation-of-microrna-132-and-its-target-gene-expression
#12
Pejman Rahimian, Johnny J He
BACKGROUND: Synaptodendritic damage is a pathological hallmark of HIV-associated neurocognitive disorders, and HIV-1 Tat protein is known to cause such injury in the central nervous system. In this study, we aimed to determine the molecular mechanisms of Tat-induced neurite shortening, specifically the roles of miR-132, an important regulator of neurite morphogenesis in this process. METHODS: The relationship between Tat expression and miR-132 expression was first determined using reverse transcription quantitative PCR (qRT-PCR) in Tat-transfected astrocytes and neurons, astrocytes from Tat-transgenic mice, and HIV-infected astrocytes...
September 15, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27580334/use-of-flaviviral-genetic-fragments-as-a-potential-prevention-strategy-for-hiv-1-silencing
#13
Muhammad Sheraz, Mazhar Kanak, Mahmudul Hasan, Roshan Bhattarai, Kuhanandha Mahalingam, Leanna A Sealey, Rashshana R Blackwood, Zhabiz Golkar, Ewen McLean, Omar Bagasra
INTRODUCTION: Coinfection with certain members of the Flaviviridae, such as Dengue Virus (DV), West Nile Virus (WNV) Yellow Fever Virus (YFV) and most importantly, GBV-C have been documented to reduce HIV-1 viral load in vivo. Numerous studies strongly support the notion that persistent coinfection with non-pathogenic virus prolongs survival in HIV-1 infected individuals. Coinfected individuals show higher CD4+ cell counts, lower HIV-1 RNA viral loads and live three times longer than clinically matched HIV-1 monoinfected patients...
August 31, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27574520/circulating-micrornas-potential-and-emerging-biomarkers-for-diagnosis-of-human-infectious-diseases
#14
REVIEW
Parmila Verma, Rajan K Pandey, Priyanka Prajapati, Vijay K Prajapati
MicroRNAs (miRNAs) are evolutionary conserved, small non-coding RNA with size ranging from 19 to 24 nucleotides. They endogenously regulate the gene expression at the post transcriptional level either through translation repression or mRNA degradation. MiRNAs have shown the potential to be used as a biomarker for the diagnosis, prognosis, and therapy of infectious diseases. Many miRNAs have shown significantly altered expression during infection. The altered expression of miRNA level in an infected human can be identified by the use of advanced diagnostic tools...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27572460/roles-of-micrornas-in-hiv-1-replication-and-latency
#15
Binlian Sun, Rongge Yang, Massimo Mallardo
MicroRNAs (miRNAs) are non-coding RNA molecules, with sequence length of 19-24 nucleotides, which can induce mRNA degradation and regulate protein translation repression. Recently plenty of reports showed that miRNAs increase or decrease in the serum (circulating miRNAs) and in PBMC of Human immunodeficiency virus type 1 (HIV-1) infected individuals to affect the replication of HIV-1 through regulating HIV-1 proteins or HIV-1 replication related host factors. Many of miRNAs can suppress HIV-1 replication, but do not affect the integrated viral DNA...
2016: MicroRNA
https://www.readbyqxmd.com/read/27538994/development-and-characterization-of-a-human-microglia-cell-model-of-hiv-1-infection
#16
Pratima Rawat, Stephen A Spector
Microglia cells are the major reservoir of HIV-1 (HIV) within the CNS. However, current models using transformed cell lines are not representative of primary microglia and fetal brain samples for isolation of primary human microglia (HMG) are increasingly difficult to obtain. Here, we describe a monocyte-derived microglia (MMG) cell model of HIV infection that recapitulates infection of primary HMG. CD14(+) cells isolated from healthy donors were cultured with M-CSF, beta-nerve growth factor, GM-CSF, and CCL2, and compared to HMG...
August 18, 2016: Journal of Neurovirology
https://www.readbyqxmd.com/read/27449085/novel-mir-122-delivery-system-based-on-ms2-virus-like-particle-surface-displaying-cell-penetrating-peptide-tat-for-hepatocellular-carcinoma
#17
Guojing Wang, Tingting Jia, Xixia Xu, Le Chang, Rui Zhang, Yu Fu, Yulong Li, Xin Yang, Kuo Zhang, Guigao Lin, Yanxi Han, Jinming Li
Current treatments for hepatocellular carcinoma (HCC) have shown inadequate. MicroRNA-122 (miR-122) mediated RNA interference brings new prospects. A safe, efficient miRNA delivery system is an indispensable assurance. Previously, we developed an MS2 bacteriophage virus-like particle (VLP)-based microRNA delivery system crosslinked with the HIV TAT peptide, which served as an effective inhibitor in the treatments of systemic lupus erythematosus and osteoporosis. However, defects, such as low crosslinking efficiency, high cost, and potential toxicity of the crosslinking agent, needed to be confronted...
September 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27440900/hiv-1-vpr-inhibits-kaposi-s-sarcoma-associated-herpesvirus-lytic-replication-by-inducing-microrna-mir-942-5p-and-activating-nf-%C3%AE%C2%BAb-signaling
#18
Qin Yan, Chenyou Shen, Jie Qin, Wan Li, Minmin Hu, Hongmei Lu, Di Qin, Jianzhong Zhu, Shou-Jiang Gao, Chun Lu
UNLABELLED: Kaposi's sarcoma-associated herpesvirus (KSHV) infection is required for the development of several AIDS-related malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). The high incidence of AIDS-KS has been ascribed to the interaction of KSHV and HIV-1. We have previously shown that HIV-1-secreted proteins Tat and Nef regulate the KSHV life cycle and synergize with KSHV oncogenes to promote angiogenesis and tumorigenesis. Here, we examined the regulation of KSHV latency by HIV-1 viral protein R (Vpr)...
October 1, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27397032/the-effect-of-different-microrna-backbones-on-artificial-mirna-expression-and-knockdown-activity-against-hiv-1-replication
#19
Ahmad Nejati, Masoud Soleimani, Ehsan Arefian, Sayed M Marashi, Hamideh Tabatabaie, Mohammad Farahmand, Zabihollah Shoja, Mahmood Mahmoodi, Rakhshandeh Nategh, Shohreh Shahmahmoodi
BACKGROUND: Artificial microRNAs (miRNAs) are designed to develop an RNAi-based gene therapy. Recently, it has been suggested that the flanking sequences and terminal loop structure play a critical role in RNAi biogenesis and target recognition, but no extensive study regarding the different miRNA backbone for artificial miRNAs optimization has been conducted. OBJECTIVE: We tested three artificial miRNAs with human hsa-miR30a (common miRNA), hsa-miR150 (T cell specific miRNA), and hsa-miR122 (liver specific miRNA) backbones in HEK-293T and Jurkat cell lines...
2016: MicroRNA
https://www.readbyqxmd.com/read/27320691/anti-hiv-microrna-expression-in-a-novel-indian-cohort
#20
Rakesh Dey, Kartik Soni, Shanmugam Saravanan, Pachamuthu Balakrishnan, Vikram Kumar, Jayaseelan Boobalan, Sunil Suhas Solomon, Vinod Scaria, Suniti Solomon, Samir K Brahmachari, Beena Pillai
HIV-1 replication inside host cells is known to be regulated by various host factors. Host miRNAs, by virtue of its normal functioning, also regulate HIV-1 RNA expression by either directly targeting virus mRNAs or indirectly by regulating host proteins that HIV-1 uses for own replication. Therefore, it is highly possible that with differential miRNA expression, rate of disease progression will vary in HIV-1 infected individuals. In this study we have compared expression of a panel of 13 reported anti-HIV miRNAs in human PBMCs from long term non progressors (LTNPs), regular progressors and rapid progressors...
2016: Scientific Reports
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