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Changwei Li, Jiang He, Jing Chen, Jinying Zhao, Dongfeng Gu, James E Hixson, Dabeeru C Rao, Cashell E Jaquish, Charles C Gu, Jichun Chen, Jianfeng Huang, Shufeng Chen, Tanika N Kelly
We performed genome-wide analyses to identify genomic loci that interact with sodium to influence blood pressure (BP) using single-marker-based (1 and 2 df joint tests) and gene-based tests among 1876 Chinese participants of the Genetic Epidemiology Network of Salt-Sensitivity (GenSalt) study. Among GenSalt participants, the average of 3 urine samples was used to estimate sodium excretion. Nine BP measurements were taken using a random zero sphygmomanometer. A total of 2.05 million single-nucleotide polymorphisms were imputed using Affymetrix 6...
August 2016: Hypertension
S Stringer, C C Minică, K J H Verweij, H Mbarek, M Bernard, J Derringer, K R van Eijk, J D Isen, A Loukola, D F Maciejewski, E Mihailov, P J van der Most, C Sánchez-Mora, L Roos, R Sherva, R Walters, J J Ware, A Abdellaoui, T B Bigdeli, S J T Branje, S A Brown, M Bruinenberg, M Casas, T Esko, I Garcia-Martinez, S D Gordon, J M Harris, C A Hartman, A K Henders, A C Heath, I B Hickie, M Hickman, C J Hopfer, J J Hottenga, A C Huizink, D E Irons, R S Kahn, T Korhonen, H R Kranzler, K Krauter, P A C van Lier, G H Lubke, P A F Madden, R Mägi, M K McGue, S E Medland, W H J Meeus, M B Miller, G W Montgomery, M G Nivard, I M Nolte, A J Oldehinkel, Z Pausova, B Qaiser, L Quaye, J A Ramos-Quiroga, V Richarte, R J Rose, J Shin, M C Stallings, A I Stiby, T L Wall, M J Wright, H M Koot, T Paus, J K Hewitt, M Ribasés, J Kaprio, M P Boks, H Snieder, T Spector, M R Munafò, A Metspalu, J Gelernter, D I Boomsma, W G Iacono, N G Martin, N A Gillespie, E M Derks, J M Vink
Cannabis is the most widely produced and consumed illicit psychoactive substance worldwide. Occasional cannabis use can progress to frequent use, abuse and dependence with all known adverse physical, psychological and social consequences. Individual differences in cannabis initiation are heritable (40-48%). The International Cannabis Consortium was established with the aim to identify genetic risk variants of cannabis use. We conducted a meta-analysis of genome-wide association data of 13 cohorts (N=32 330) and four replication samples (N=5627)...
2016: Translational Psychiatry
Giulia Barbieri, Alessandra M Albertini, Eugenio Ferrari, Abraham L Sonenshein, Boris R Belitsky
UNLABELLED: AprE and NprE are two major extracellular proteases in Bacillus subtilis whose expression is directly regulated by several pleiotropic transcriptional factors, including AbrB, DegU, ScoC, and SinR. In cells growing in a rich, complex medium, the aprE and nprE genes are strongly expressed only during the post-exponential growth phase; mutations in genes encoding the known regulators affect the level of post-exponential-phase gene expression but do not permit high-level expression during the exponential growth phase...
March 2016: Journal of Bacteriology
Pinar Pazarli Bostan, Canan Karaman Demir, Osman Elbek, Şule Akçay
BACKGROUND: A strategy to reduce the number of smoking-related deaths is to encourage the involvement of health-care professionals in tobacco-use prevention activities and cessation counseling. Previous studies have shown that physicians' smoking status affects their efforts to provide smoking cessation counseling. This study investigates the association between pulmonologists' tobacco use and their efforts in promoting smoking cessation during their routine clinical practices in Turkey...
2015: BMC Pulmonary Medicine
Boris R Belitsky, Shaun R Brinsmade, Abraham L Sonenshein
The global transcriptional regulator, CodY, binds strongly to the regulatory region of the braB gene, which encodes a Bacillus subtilis branched-chain amino acid (BCAA) permease. However, under conditions that maximize CodY activity, braB expression was similar in wild-type and codY null mutant cells. Nonetheless, expression from the braB promoter was significantly elevated in cells containing partially active mutant versions of CodY or in wild-type cells under growth conditions leading to intermediate levels of CodY activity...
October 2015: PLoS Genetics
Burcu Şahin, Sibel Öztürk, Pınar Çalık, Tunçer H Özdamar
Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC (-)) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and (NH4)2HPO4 at two pre-determined specific growth rates, µ 0 = 0.10 and 0.17 h(-1). Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, (NH4)2HPO4, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ 0 = 0...
October 2015: Bioprocess and Biosystems Engineering
Abderahmane Derouiche, Lei Shi, Vladimir Bidnenko, Magali Ventroux, Nathalie Pigonneau, Mirita Franz-Wachtel, Aida Kalantari, Sylvie Nessler, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis Mrp family protein SalA has been shown to indirectly promote the production of the exoprotease AprE by inhibiting the expression of scoC, which codes for a repressor of aprE. The exact mechanism by which SalA influences scoC expression has not been clarified previously. We demonstrate that SalA possesses a DNA-binding domain (residues 1-60), which binds to the promoter region of scoC. The binding of SalA to its target DNA depends on the presence of ATP and is stimulated by phosphorylation of SalA at tyrosine 327...
September 2015: Molecular Microbiology
Jinyuan Yan, Wei Zou, Juan Fang, Xiaowei Huang, Feng Gao, Zeying He, Keqin Zhang, Ninghui Zhao
Protein kinase A (PrkA), also known as AMP-activated protein kinase, functions as a serine/threonine protein kinase (STPK), has been shown to be involved in a variety of important biologic processes, including pathogenesis of many important diseases in mammals. However, the biological functions of PrkA are less known in prokaryote cells. Here, we explored the function of PrkA as well as its underlying molecular mechanisms using the model bacterium Bacillus subtilis168. When PrkA is inhibited by 9-β-D-arabinofuranosyladenine (ara-A) in the wild type strain or deleted in the ΔprkA mutant strain, we observed sporulation defects in B...
2015: Frontiers in Microbiology
Boris R Belitsky, Giulia Barbieri, Alessandra M Albertini, Eugenio Ferrari, Mark A Strauch, Abraham L Sonenshein
CodY and ScoC are Bacillus subtilis transcriptional regulators that control the expression of dozens of genes and operons. Using scoC-lacZ fusions and DNA-binding experiments, we show here that scoC is directly repressed by CodY. This effect creates multiple forms of cascade regulation. For instance, expression of the dtpT gene, which is directly and negatively controlled by ScoC and encodes a putative oligopeptide permease, was activated indirectly by CodY due to CodY-mediated repression of scoC. The opp operon, which encodes an oligopeptide permease that is essential for sporulation and genetic competence development, proved to be a direct target of repression by both ScoC and CodY but was not significantly affected in codY or scoC single mutants...
August 2015: Molecular Microbiology
Milena B Ilić, Dalibor V Jovanović, Miloš Z Milosavljević, Vesna Stanković, Gordana Djordjević, Zoran Protrka, Jasmina Nedović, Slobodanka Lj Mitrović
INTRODUCTION: Extrapulmonary small cell carcinoma is a rare, prognostically bad tumor category. Primary, it can be localized in every organ, even in the ovary, where, due to its clinical specificities, it represents a challenge in diagnosis, as well as in therapy. Small cell ovarian carcinoma (SCOC) is biologically very aggressive malignant tumor of unknown histogenesis. We presented a rare case of SCOC with hypercalcemia of aggressive course and fatal outcome in a postmenopausal woman at International Federation of Gynecology and Obstetrics (FIGO) Ia stage...
March 2015: Vojnosanitetski Pregled. Military-medical and Pharmaceutical Review
Majed Sammak, Egill Thorbergsson, Tomas Grönstedt, Magnus Genrup
The aim of this study was to compare single- and twin-shaft oxy-fuel gas turbines in a semiclosed oxy-fuel combustion combined cycle (SCOC-CC). This paper discussed the turbomachinery preliminary mean-line design of oxy-fuel compressor and turbine. The conceptual turbine design was performed using the axial through-flow code luax-t, developed at Lund University. A tool for conceptual design of axial compressors developed at Chalmers University was used for the design of the compressor. The modeled SCOC-CC gave a net electrical efficiency of 46% and a net power of 106 MW...
August 2013: Journal of Engineering for Gas Turbines and Power
Ulrike Braeckman, Carl Van Colen, Katja Guilini, Dirk Van Gansbeke, Karline Soetaert, Magda Vincx, Jan Vanaverbeke
Research so far has provided little evidence that benthic biogeochemical cycling is affected by ocean acidification under realistic climate change scenarios. We measured nutrient exchange and sediment community oxygen consumption (SCOC) rates to estimate nitrification in natural coastal permeable and fine sandy sediments under pre-phytoplankton bloom and bloom conditions. Ocean acidification, as mimicked in the laboratory by a realistic pH decrease of 0.3, significantly reduced SCOC on average by 60% and benthic nitrification rates on average by 94% in both sediment types in February (pre-bloom period), but not in April (bloom period)...
2014: PloS One
Pınar Pazarli Bostan, Osman Elbek, Oğuz Kilinç, Müşerref Şule Akçay, Sibel Kiran
INTRODUCTION: Although helping patients to stop smoking is a good clinical practice that has to be carried out by physicians everywhere and in every area, it is known that carrying out this help systematically in accordance to a programme in smoking cessation outpatient clinics significantly improves the chance of success. MATERIALS AND METHODS: The study is a cross-sectional survey performed among chest disease specialists who are members of Turkish Thoracic Society (TTS), between June 2010 and February 2011...
2014: Tüberküloz Ve Toraks
K Münstedt, R Estel, T Dreyer, A Kurata, A Benz
Objective: Small cell ovarian carcinomas (SCOC) are differentiated into two types: hypercalcaemic (SCOCHT) and pulmonary (SCOCPT). Unfortunately, little is known about pulmonary-type small cell ovarian carcinoma. Study Design: We carried out a systematic analysis of all available reports in the literature on individual cases of SCOCHT and SCOCPT. Results: We found that patients with SCOCPT were significantly older than those with SCOCHT. Vimentin and chromogranin detection by immunohistochemistry allow good differentiation between the two types...
July 2013: Geburtshilfe und Frauenheilkunde
Carol Vargas-Bautista, Kathryn Rahlwes, Paul Straight
Bacillus subtilis is adaptable to many environments in part due to its ability to produce a broad range of bioactive compounds. One such compound, bacillaene, is a linear polyketide/nonribosomal peptide. The pks genes encode the enzymatic megacomplex that synthesizes bacillaene. The majority of pks genes appear to be organized as a giant operon (>74 kb from pksC-pksR). In previous work (P. D. Straight, M. A. Fischbach, C. T. Walsh, D. Z. Rudner, and R. Kolter, Proc. Natl. Acad. Sci. U. S. A. 104:305-310, 2007, doi:10...
February 2014: Journal of Bacteriology
Caroline Behrens, Beyenech Binotti, Carla Schmidt, Carol V Robinson, John Jia En Chua, Karin Kühnel
The short coiled coil protein (SCOC) forms a complex with fasciculation and elongation protein zeta 1 (FEZ1). This complex is involved in autophagy regulation. We determined the crystal structure of the coiled coil domain of human SCOC at 2.7 Å resolution. SCOC forms a parallel left handed coiled coil dimer. We observed two distinct dimers in the crystal structure, which shows that SCOC is conformationally flexible. This plasticity is due to the high incidence of polar and charged residues at the core a/d-heptad positions...
2013: PloS One
Monica Gupta, Madhulika Dixit, K Krishnamurthy Rao
Bacillus subtilis under nutritional deprivation exhibits several physiological responses such as synthesis of degradative enzymes, motility, competence, sporulation, etc. At the onset of post-exponential phase the global response regulator, Spo0A, directly or indirectly activates the expression of genes involved in the above processes. These genes are repressed during the exponential phase by a group of proteins called transition state regulators, e.g. AbrB, ScoC and SinR. One such post-exponentially expressed gene is epr, which encodes a minor extracellular serine protease and is involved in the swarming motility of B...
June 2013: Journal of Biosciences
Justin Joachim, Martina Wirth, Nicole C McKnight, Sharon A Tooze
Autophagy is a conserved and highly regulated catabolic pathway, transferring cytoplasmic components in autophagosomes to lysosomes for degradation and providing amino acids during starvation. In multicellular organisms autophagy plays an important role for tissue homeostasis, and deregulation of autophagy has been implicated in a broad range of diseases, including cancer and neurodegenerative disorders. In mammals, many aspects of autophagy still need to be fully elucidated: what is the exact hierarchy and relationship between ATG proteins and other factors that lead to the formation and expansion of phagophores? Where does the membrane source for autophagosome formation originate? Which signaling events trigger amino acid starvation-induced autophagy? How are the activities of ULK1/2 and the class III PtdIns3K regulated and linked to each other? To develop therapeutic strategies to manipulate autophagy in human disease, a comprehensive understanding of the molecular protein machinery mediating and regulating autophagy is required...
September 2012: Autophagy
Aruljothi Mariappan, Oliwia Makarewicz, Xiao-Hua Chen, Rainer Borriss
The plant-growth-promoting-rhizobacteria Bacillus amyloliquefaciens FZB42 possess an enormous potential to synthesize a wide range of antimicrobial, antiviral and nematocidal compounds. One of them, the dipeptide antibiotic bacilysin, is synthesized by FZB42 during exponential growth. Here, we have demonstrated that bacilysin is positively regulated by the two-component response regulator DegU at the transcriptional level. In addition, ScoC (Hpr), a transition state regulator, negatively controlled expression of the bacA gene, which is the first gene within the bacilysin operon...
2012: Journal of Molecular Microbiology and Biotechnology
Nicole C McKnight, Harold B J Jefferies, Endalkachew A Alemu, Rebecca E Saunders, Michael Howell, Terje Johansen, Sharon A Tooze
Autophagy is a catabolic process by which cytoplasmic components are sequestered and transported by autophagosomes to lysosomes for degradation, enabling recycling of these components and providing cells with amino acids during starvation. It is a highly regulated process and its deregulation contributes to multiple diseases. Despite its importance in cell homeostasis, autophagy is not fully understood. To find new proteins that modulate starvation-induced autophagy, we performed a genome-wide siRNA screen in a stable human cell line expressing GFP-LC3, the marker-protein for autophagosomes...
April 18, 2012: EMBO Journal
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