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https://www.readbyqxmd.com/read/29346685/metabolic-flux-engineering-of-cembratrien-ol-production-in-both-the-glandular-trichome-and-leaf-mesophyll-in-nicotiana-tabacum
#1
Hongying Zhang, Songtao Zhang, Yongxia Yang, Hongfang Jia, Hong Cui
Cembratrien-ol synthase (CBTS) catalyses the first step in cembranoid biosynthesis, producing cembratrien-ols in plant trichomes. In our previous study, microarray transcriptomes between leaves with trichomes and leaves without trichomes showed that a NtCBTS2 gene was expressed exclusively and abundantly in trichomes. Here, two NtCBTS2 isogenes (NtCBTS2a and NtCBTS2b), derived from a diploid genome donor, N. sylvestris, were identified from N. tabacum. Both genes were expressed primarily in trichomes, with relatively decreased transcription in flowers and stems, faint expression in roots, and no expression was detected in leaves lacking trichomes...
January 15, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29343293/identification-of-preoperative-prediction-factors-of-tumor-subtypes-for-patients-with-solitary-ground-glass-opacity-pulmonary-nodules
#2
Meishuang Li, Yanan Wang, Yulong Chen, Zhenfa Zhang
BACKGROUND: Recent wide spread use of low-dose helical computed tomography for the screening of lung cancer have led to an increase in the detection rate of very faint and smaller lesions known as ground-glass opacity nodules. The purpose of this study was to investigate the clinical factors of lung cancer patients with solitary ground-glass opacity pulmonary nodules on computed tomography. METHODS: A total of 423 resected solitary ground-glass opacity nodules were retrospectively evaluated...
January 17, 2018: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/29334539/gelatin-based-hemostatic-agents-histopathologic-differences
#3
Natalie Wolkow, Frederick A Jakobiec, Michael K Yoon
PURPOSE: To delineate the histopathologic appearance of gelatin-based hemostatic agents, Surgiflo, Gelfoam, and Floseal, which are used by ophthalmic plastic surgeons, and which may incidentally be found as foreign materials in histopathologic tissue samples. METHODS: Histopathologic analysis was performed with hematoxylin-eosin, periodic acid-Schiff, Masson trichrome, and elastin staining on tissue samples in which gelatin-based agents were found. To better characterize these materials, similar analyses were performed on in vitro samples of commonly used gelatin-based hemostatic agents...
January 12, 2018: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29330272/spontaneous-splenic-rupture-as-a-rare-complication-of-g-csf-injection
#4
Soraya Benguerfi, Fanny Thepault, Hervé Lena, Charles Ricordel
Splenic rupture is an infrequent and underdiagnosed side effect of granylocyte colony-stimulating factor (G-CSF). We report the case of a 54-year-old woman with brain and bone metastasis in a lung adenocarcinoma who was admitted for faintness 28 days after a G-CSF injection. Abdominal CT scan confirmed the diagnosis of splenic rupture. A conservative treatment was chosen using a peritoneal cleansing during laparoscopic surgery. Clinicians should be aware of this rare toxicity as it could be severe, but easily reversible using appropriate surgical treatment...
January 12, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29321670/clinical-and-experimental-evidence-suggest-a-link-between-kif7-and-c5orf42-related-ciliopathies-through-sonic-hedgehog-signaling
#5
Reza Asadollahi, Justin E Strauss, Martin Zenker, Oliver Beuing, Simon Edvardson, Orly Elpeleg, Tim M Strom, Pascal Joset, Dunja Niedrist, Christine Otte, Beatrice Oneda, Paranchai Boonsawat, Silvia Azzarello-Burri, Deborah Bartholdi, Michael Papik, Markus Zweier, Cordula Haas, Arif B Ekici, Alessandra Baumer, Eugen Boltshauser, Katharina Steindl, Michael Nothnagel, Albert Schinzel, Esther T Stoeckli, Anita Rauch
Acrocallosal syndrome (ACLS) is an autosomal recessive neurodevelopmental disorder caused by KIF7 defects and belongs to the heterogeneous group of ciliopathies related to Joubert syndrome (JBTS). While ACLS is characterized by macrocephaly, prominent forehead, depressed nasal bridge, and hypertelorism, facial dysmorphism has not been emphasized in JBTS cohorts with molecular diagnosis. To evaluate the specificity and etiology of ACLS craniofacial features, we performed whole exome or targeted Sanger sequencing in patients with the aforementioned overlapping craniofacial appearance but variable additional ciliopathy features followed by functional studies...
January 10, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29289191/surface-loop-gap-resonators-for-electron-spin-resonance-at-w-band
#6
Ygal Twig, Anton Sorkin, David Cristea, Akiva Feintuch, Aharon Blank
Electron spin resonance (ESR) is a spectroscopic method used to detect paramagnetic materials, reveal their structure, and also image their position in a sample. ESR makes use of a large static magnetic field to split the energy levels of the electron magnetic moment of the paramagnetic species. A strong microwave magnetic field is applied to excite the spins, and subsequently the ESR system detects their faint microwave signal response. The sensitivity of an ESR system is greatly influenced by the magnitude of the static field and the properties of the microwave resonator used to detect the spin signal...
December 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29277068/biological-effects-of-polyphenol-rich-extract-and-fractions-from-an-oenological-oak-derived-tannin-on-in%C3%A2-vitro-swine-sperm-capacitation-and-fertilizing-ability
#7
Marcella Spinaci, Vera Muccilli, Diego Bucci, Nunzio Cardullo, Beatrice Gadani, Corrado Tringali, Carlo Tamanini, Giovanna Galeati
Although excessive ROS levels induce sperm damage, sperm capacitation is an oxidative event that requires low amounts of ROS. As the antioxidant activity of the ethanol extract (TRE) of a commercial oenological tannin (Quercus robur toasted oak wood, Tan'Activ R®) and its four fractions (FA, FB, FC, FD) has been recently reported, the present study was set up to investigate the biological effects of TRE and its fractions in an in vitro model of sperm capacitation and fertilization. Boar sperm capacitation or gamete coincubation were performed in presence of TRE or its fractions (0, 1, 10, 100 μg/ml)...
December 14, 2017: Theriogenology
https://www.readbyqxmd.com/read/29276030/the-impact-of-prehospital-assessment-and-ems-transport-of-acute-aortic-syndrome-patients
#8
Akira Yamashita, Tetsuo Maeda, Yoshihito Kita, Satoru Sakagami, Yasuhiro Myojo, Yukihiro Wato, Yutaka Yoshita, Hideo Inaba
BACKGROUND: The quality of acute aortic syndrome (AAS) assessment by emergency medical service (EMS) and the incidence and prehospital factors associated with 1-month survival remain unclear. METHODS: We retrospectively analyzed the data collected for 94,468 patients with non-traumatic medical emergency excluding out-of-hospital cardiac arrest during the period of 2011-2014. RESULTS: Of these transported by EMS, 22,075 had any of the AAS-related symptoms, and 330 had an EMS-assessed risk for AAS; of these, 195 received an in-hospital AAS diagnosis...
December 5, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29260437/time-perception-is-not-for-the-faint-hearted-physiological-arousal-does-not-influence-duration-categorisation
#9
Valérie Dormal, Alexandre Heeren, Mauro Pesenti, Pierre Maurage
Distortions of duration perception provoked by emotion-induced arousal changes are explained by modifications of an internal clock pace. Yet, uncertainty still abounds regarding whether changes of arousal induced by physical exercise yield such temporal distortions. Here, we report two experiments aiming to test separately the impact of, on the one hand, a physical induction of arousal and, on the other hand, a task delay on duration categorisation. In Experiment 1, participants performed a duration categorisation task before and after heart-rate manipulation (increase, decrease, or no change)...
December 20, 2017: Cognitive Processing
https://www.readbyqxmd.com/read/29260069/atypical-intravitreal-growth-of-retinoblastoma-with-a-multi-branching-configuration
#10
Sachiko Nishina, Satoshi Katagiri, Atsuko Nakazawa, Chikako Kiyotani, Tadashi Yokoi, Noriyuki Azuma
Purpose: To report the clinical and histopathological findings of atypical intravitreal growth of a retinoblastoma with a multi-branching configuration. Observations: A 7-month-old boy was referred to our hospital due to leukocoria in the right eye. Ophthalmic examinations identified multi-branching vessels surrounded by diaphanous tissue behind the lens in the right eye. Imaging modalities showed microphthalmos, band-shaped calcification, and cystic lesions in that eye...
September 2017: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29248133/hodgkin-lymphoma-a-complex-metabolic-ecosystem-with-glycolytic-reprogramming-of-the-tumor-microenvironment
#11
Lekha Mikkilineni, Diana Whitaker-Menezes, Marina Domingo-Vidal, John Sprandio, Paola Avena, Paolo Cotzia, Alina Dulau-Florea, Jerald Gong, Guldeep Uppal, Tingting Zhan, Benjamin Leiby, Zhao Lin, Barbara Pro, Federica Sotgia, Michael P Lisanti, Ubaldo Martinez-Outschoorn
BACKGROUND: Twenty percent of patients with classical Hodgkin Lymphoma (cHL) have aggressive disease defined as relapsed or refractory disease to initial therapy. At present we cannot identify these patients pre-treatment. The microenvironment is very important in cHL because non-cancer cells constitute the majority of the cells in these tumors. Non-cancer intra-tumoral cells, such as tumor-associated macrophages (TAMs) have been shown to promote tumor growth in cHL via crosstalk with the cancer cells...
June 2017: Seminars in Oncology
https://www.readbyqxmd.com/read/29247380/utilizing-mass-spectrometry-imaging-to-map-the-thyroid-hormones-triiodothyronine-and-thyroxine-in-xenopus-tropicalis-tadpoles
#12
Naoko Goto-Inoue, Tomohiko Sato, Mizuki Morisasa, Akihiko Kashiwagi, Keiko Kashiwagi, Yuki Sugiura, Eiji Sugiyama, Makoto Suematsu, Tsukasa Mori
Thyroid hormones are not only responsible for thermogenesis and energy metabolism in animals, but also have an important role in cell differentiation and development. Amphibian metamorphosis provides an excellent model for studying the remodeling of the body. This metamorphic organ remodeling is induced by thyroid hormones, and a larval body is thus converted into an adult one. The matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) imaging technology is expected to be a suitable tool for investigating small bioreactive molecules...
December 15, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29246931/periosteal-desmoid-tumour-a-rare-finding-in-the-oral-cavity
#13
Singh Shruti, Radhika M Bavle, Paremala Konda, Reshma Venugopal
A 55-year-old female patient reported with an intraoral well-localised asymptomatic swelling on the right side of the mandible in relation to the right mandibular first molar along with a history of trauma 6 months back. Panoramic radiograph revealed normal trabecular bone pattern in relation to the lesion. The lesion was excised along with the associated buccal cortical plate and tooth. The microscopic examination revealed a well-circumscribed lesion consisting of spindle cells arranged in storiform pattern associated with the buccal cortical plate...
December 14, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29246835/brief-report-pd-l1-expression-of-tumor-cells-macrophages-and-immune-cells-in-non-small-cell-lung-cancer-patients-with-malignant-pleural-effusion
#14
Yen-Han Tseng, Hsiang-Ling Ho, Chiung-Ru Lai, Yung-Hung Luo, Yen-Chiang Tseng, Jacqueline Whang-Peng, Yi-Hsuan Lin, Teh-Ying Chou, Yuh-Min Chen
INTRODUCTION: Weather we could use IHC stain of PD-L1 on cells of pleural effusion to predict immunotherapy treatment response have not been reported. METHODS: We retrospectively enrolled patients who had undergone malignant pleural effusion drainage and had effusion cell block specimens from 2014-2016. IHC staining for PD-L1 was performed with tumor cells, immune cells, and macrophage of all cell block specimens. The immunoactivity was defined as 0 for absence of staining; 1+ for faint; 2+ for moderate; 3+ for intense membranous staining...
December 12, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/29245682/a-new-i-schistura-i-from-an-upper-mekong-affluent-in-yunnan-teleostei-nemacheilidae
#15
Marco Endruweit
Schistura stala, new species, is described from the Mekong drainage in Dali, Yunnan Province, China. The new species is readily distinguished from congeners in the upper Mekong and adjacent drainages by having the caudal-fin bar reduced, stretching over the median ⅓ of the fin base. The species can be further diagnosed by a deep body; a short caudal fin, head and pelvic fin; 38 vertebrae modally; 9-12 faint irregular flank bars, that are often anastomotic or dissolved; and few scales scattered on the anterior part of the body...
November 1, 2017: Zootaxa
https://www.readbyqxmd.com/read/29245551/descriptions-of-three-new-species-of-the-genus-i-symmorphus-i-wesmael-1836-hymenoptera-vespidae-eumeninae-from-south-korea-with-an-updated-revised-key-to-far-eastern-species
#16
Jeong-Kyu Kim
Three new species of the genus Symmorphus Wesmael, 1836 are described with materials from South Korea and Far eastern Russia: Symmorphus kurzenkoi Kim sp. nov., S. truncatoclypeus Kim sp. nov., S. yamanei Kim sp. nov. All are members of the subgenus Symmorphus Wesmael 1836. S. kurzenkoi Kim sp. nov. is likely to be closely related to Symmorphus fuscipes in having well developed and completely closed propodeal submedian carina, but is clearly different from the latter in the shape of metasomal tergum 1 that is not markedly narrowed toward base...
November 17, 2017: Zootaxa
https://www.readbyqxmd.com/read/29245429/-i-macrobiotus-i-i-nebrodensis-i-and-i-adropion-i-i-vexatum-i-two-new-species-of-eutardigrada-tardigrada-parachela-from-sicily
#17
Giovanni Pilato, Giorgio Sabella, Vera D'Urso, Oscar Lisi
Two new species of Eutardigrada are described from Sicily: Macrobiotus nebrodensis sp. nov. and Adropion vexatum sp. nov. The former species, belongs to the Macrobiotus hufelandi group, and is characterised by having a small, faint, microplacoid, and variable egg processes (most processes are shaped in the form of an inverted goblet with jagged terminal disc, but others are long, or very long, with a very small terminal disc). Adropion vexatum sp. nov. is characterised by having smooth cuticle, steep, vertical front to the head, pharyngeal tube longer than the pharyngeal bulb, which has small apophyses and three rod-shaped macroplacoids (microplacoid and septulum absent); long placoid row, about half the length of the pharyngeal bulb; main branches of claws with accessory points; lunules and other cuticular thickenings absent on the legs (small dots excluded)...
December 5, 2017: Zootaxa
https://www.readbyqxmd.com/read/29245346/two-independent-incidences-of-skin-metastases-in-the-umbilicus-and-abdominal-wall-in-ovarian-serous-adenocarcinoma-a-case-report-and-review-of-the-literature
#18
Hongyan Cheng, Chunmei Gao, Runtong Zhang, Zhaojie Yang, Guiyu Zhang
RATIONALE: Advanced ovarian cancer is usually associated with intra-abdominal metastases and while it commonly spreads directly to the omentum, intestine, liver, or other organs, it can also metastasize through the lymphatic channels and the hematogenous pathway. With an increasing number of invasive operations being performed with chemoradiotherapy, the incidence of extra-abdominal metastases has risen. Nevertheless, ovarian cancer with skin metastases is quite rare. PATIENT CONCERNS: We report a case of ovarian cancer with two independent incidences of skin metastases in the umbilicus and abdominal wall...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29243813/antiepileptic-drug-monotherapy-for-epilepsy-a-network-meta-analysis-of-individual-participant-data
#19
REVIEW
Sarah J Nevitt, Maria Sudell, Jennifer Weston, Catrin Tudur Smith, Anthony G Marson
BACKGROUND: Epilepsy is a common neurological condition with a worldwide prevalence of around 1%. Approximately 60% to 70% of people with epilepsy will achieve a longer-term remission from seizures, and most achieve that remission shortly after starting antiepileptic drug treatment. Most people with epilepsy are treated with a single antiepileptic drug (monotherapy) and current guidelines from the National Institute for Health and Care Excellence (NICE) in the United Kingdom for adults and children recommend carbamazepine or lamotrigine as first-line treatment for partial onset seizures and sodium valproate for generalised onset seizures; however a range of other antiepileptic drug (AED) treatments are available, and evidence is needed regarding their comparative effectiveness in order to inform treatment choices...
December 15, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29235537/optical-sparse-aperture-imaging-with-faint-objects-using-improved-spatial-modulation-diversity
#20
Zongliang Xie, Haotong Ma, Bo Qi, Ge Ren, Xiaojun He, Li Dong, Yufeng Tan, Shan Qiao
The next generation of optical sparse aperture systems will provide high angular resolution for astronomical observations. Spatial modulation diversity (SMD) is a newly developed post-processing technique for such telescopes, faced with challenges of imaging faint objects, which are very attractive for astronomers but always make raw diversity images suffer serious photon noise. In this paper, we propose an improved SMD with denoising reprocessing embedded to address the problem. The blocking-matching and 3D filtering algorithm, a state-of-the-art denoising technique, is first employed to process the diversity images with low photon intensities generated by spatial modulation, specifically switching off each sub-aperture sequentially...
December 13, 2017: Scientific Reports
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