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https://www.readbyqxmd.com/read/28738348/colistin-resistance-in-kpc-2-and-shv-5-producing-klebsiella-pneumoniae-clinical-isolates-in-bulgaria
#1
Encho Savov, Iva Todorova, Lida Politi, Angelina Trifonova, Maja Borisova, Elena Kioseva, Athanassios Tsakris
BACKGROUND/AIMS: Colistin resistance is increasingly recognized among carbapenemase-producing Klebsiella pneumoniae isolates in several European regions. The current study documents the appearance of colistin resistance among KPC-2 and SHV-5-produning K. pneumoniae strains in Bulgaria. METHODS: Four colistin-resistant K. pneumoniae isolates were recovered from 2 patients hospitalized in the anesthesiology and resuscitation clinic of a tertiary care university hospital in Sofia, Bulgaria...
July 22, 2017: Chemotherapy
https://www.readbyqxmd.com/read/28738194/characterization-and-horizontal-transfer-of-qach-associated-class-1-integrons-in-escherichia-coli-isolated-from-retail-meats
#2
Xiaobing Jiang, Yameng Xu, Yi Li, Kun Zhang, Lei Liu, Hailei Wang, Jinhe Tian, Hao Ying, Lei Shi, Tao Yu
The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Escherichia coli isolated from retail meats. Among the 179 E. coli isolates tested, the minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to 64μg/mL. PCR assays indicated that QAC-resistance genes sugE(c), ydgE/ydgF, mdfA, emrE and qacEΔ1 were commonly present (40.2%-88.3%) in these isolates, but qacE, qacF, qacH and sugE(p) were less prevalent (2...
July 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28737824/inhibitory-effects-of-1-25-oh-2d3-on-the-proliferation-of-hepatocellular-carcinoma-cells-through-the-downregulation-of-hdac2
#3
Jian Huang, Guozhen Yang, Yunzhu Huang, Shu Zhang
The inhibitory effects of 1,25(OH)2D3 on the proliferation of a variety of cancer cell lines have been extensively reported. However, the underlying mechanisms remain largely unknown. In the present study, the effects of 1,25(OH)2D3 on the in vitro proliferation of human hepatocellular carcinoma HepG2 cells and the mechanism involved were investigated. Flow cytometry and MTT assay revealed that 1,25(OH)2D3 inhibited cell proliferation in vitro. Western blotting and real-time PCR indicated that 1,25(OH)2D3 upregulated the expression of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) and attenuated that of histone deacetylase 2 (HDAC2)...
July 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28737463/prevalence-of-a-new-variant-oxa-204-and-oxa-48-carbapenemases-plasmids-encoded-in-klebsiella-pneumoniae-clinical-isolates-in-tunisia
#4
Rym Ouertani, Mariem Ben Jomàa-Jemili, Haythem Gharsa, Anne Limelette, Thomas Guillard, Lucien Brasme, Christophe de Champs, Chedly Chouchani
Carbapenemase-producing Klebsiella pneumoniae strains have emerged as a major problem for healthcare systems. The aim of this study was to determine the role and diversity of plasmids harboring carbapenemases encoding genes from a collection of K. pneumoniae isolates recovered between July 2011 and January 2012, with decreased susceptibility to carbapenems. Imipenem (IPM), ertapenem (ETP), meropenem (MEM), and doripenem (DOR) minimum inhibitory concentrations (MICs) were determined by E-test. Carbapenemase production was detected with the modified Hodge test...
July 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28736549/cloning-expression-isotope-labeling-and-purification-of-transmembrane-protein-merf-from-mercury-resistant-enterobacter-sp-az-15-for-nmr-studies
#5
Aatif Amin, Zakia Latif
Mercury resistant (Hg(R)) Enterobacter sp. AZ-15 was isolated from heavy metal polluted industrial wastewater samples near to districts Kasur and Sheikhupura, Pakistan. 16S rDNA ribotyping and phylogentic analysis showed 98% homology with already reported Enterobacter species. The merF gene encoding transmembrane protein-MerF was amplified from genomic DNA and ligated into pET31b+ vector using restriction endonucleases, SphI and XhoI. The genetic codons of merF gene encoding cysteine residues were mutated into codons, translating into serine residues by site-directed mutagenesis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28736155/integrin-linked-kinase-regulates-the-transcription-of-aqp2-by-nfatc3
#6
Marco Hatem-Vaquero, Mercedes Griera, Wieslawa Giermakowska, Alicia Luengo, Laura Calleros, Laura V Gonzalez Bosc, Diego Rodríguez-Puyol, Manuel Rodríguez-Puyol, Sergio De Frutos
Two processes are associated with progressive loss of renal function: 1) decreased aquaporin-2 (AQP2) expression and urinary concentrating capacity (Nephrogenic Diabetes Insipidus, NDI); and 2) changes in extracellular matrix (ECM) composition, e.g. increased collagen I (Col I) deposition, characteristic of tubule-interstitial fibrosis. AQP2 expression is regulated by both the ECM-to-intracellular scaffold protein integrin-linked kinase (ILK) by NFATc/AP1 and other transcription factors. In the present work, we used in vivo and in vitro approaches to examine ILK participation in NFATc3/AP-1-mediated increases in AQP2 gene expression...
July 20, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28735486/detection-and-quantification-of-acute-myeloid-leukemia-associated-fusion-transcripts
#7
Jonathan Schumacher, Philippe Szankasi, Todd W Kelley
Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR)-based detection of abnormal fusion transcripts is an important strategy for the diagnosis and monitoring of patients with acute myeloid leukemia (AML) with t(8;21)(q22;q22); RUNX1-RUNX1T1, inv(16)(p13.1;q22); CBFB-MYH11 or t(15;17)(q22;q12); PML-RARA. In RT-qPCR assays, patient-derived cDNA is subjected to amplification using PCR primers directed against the fusion transcript of interest as well as a reference gene for normalization...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735317/genetic-characterization-of-a-novel-composite-transposon-carrying-arma-and-aac-6-ib-genes-in-an-escherichia-coli-isolate-from-egypt
#8
Mona T Kashef, Omneya M Helmy
Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, aminoglycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the frequency of 16S-RMTase among third generation cephalosporin-resistant clinical isolates in Egypt...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735083/incidence-of-methicillin-resistant-staphylococcus-aureus-mrsa-in-microbial-community-of-cancer-patients-and-evaluation-of-their-resistant-pattern
#9
Mervat Morsy Abbas Ahmed El-Gendy, Ahmed Mohamed Ahmed El-Bondkly, Abeer Ali Keera, Amal Mohamed Ali
Cancer patients are prone to MRSA infection due to a number of factors include prolongedhospitalization, intravascular catheterization, compromised host immunity, malignancy, chemotherapy, radiotherapy surgery, prior antibiotic therapy and prolonged operative time. Among 468 Staphylococcus isolates, 283 were methicillin resistant Staphylococcus aureus isolates (MRSA), which indicated the occurrence and prevalence of MRSA isolates among cancer patients. The highest number of MRSA strains were isolated from male (124 isolates), female (90 isolates) followed by children (69 isolates) which represent 43...
July 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28735058/prevalence-of-pmqr-genes-in-e-coli-and-klebsiella-spp-isolated-from-north-west-of-morocco
#10
Houda Benaicha, Said Barrijal, Fatima Ezzakkioui, Fatima Elmalki
OBJECTIVES: Plasmid mediated quinolone resistance (PMQR) genes are increasingly detected worldwide. However, in Morocco a few studies have been carried out. The aim of this study was to investigate the prevalence of these determinants among Escherichia coli and Klebsiella spp isolated from North-West of Morocco. METHODS: The prevalence of PMQR genes was investigated among 398 E. coli and 118 Klebsiella spp clinical isolates collected between 2012 and 2015 from North-West of Morocco...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28735002/immobilization-of-%C3%AE-galactosidase-on-surface-modified-cobalt-multiwalled-carbon-nanotube-nanocomposite-improves-enzyme-stability-and-resistance-to-inhibitor
#11
Maryam Khan, Qayyum Husain, Rani Bushra
The present study aimed to work out a high yield procedure for immobilization of Aspergillus oryzae β-galactosidase on polyaniline cobalt multiwalled carbon nanotubes nanocomposite (PANI/Co/MWCNTNC) by physical adsorption and covalent attachment via glutaraldehyde. The binding was confirmed by scanning and transmission electron microscopy along with Fourier transform-infrared spectroscopy. The immobilization yields obtained for adsorbed and cross-linked enzymes were 93% and 97%, respectively. The covalently immobilized enzyme was remarkably more stable at extremes of pH and temperature compared to native and adsorbed enzymes...
July 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28734743/adjuvantation-of-inactivated-foot-and-mouth-disease-virus-vaccine-with-il-15-expressing-plasmid-improves-the-immune-response-in-guinea-pigs
#12
Vijay Nagaraj, Lijo John, Singaram Bharatiraja, Hosur Joyappa Dechamma, Golla Ramalinga Reddy
Foot and mouth disease is a highly contagious disease affecting cloven footed animals. Vaccination using inactivated virus is followed to control the disease. As the immune response conferred by the inactivated vaccine is short lived, there is a need for an alternate vaccine with increased duration of immunity. Inclusion of adjuvant which enhances B and T cell responses is one of the strategies to increase the duration of immune responses of the vaccine. Interleukin 15 is one such a cytokine which improves the cell mediated immune response and also involved in the maintenance of memory T and B cells...
July 19, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28734577/characterization-of-a-pvl-negative-community-acquired-methicillin-resistant-staphylococcus-aureus-strain-of-sequence-type-88-in-china
#13
Lu Sun, Dandan Wu, Yan Chen, Qian Wang, Haiping Wang, Yunsong Yu
Sequence type 88 community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) strain SR434, isolated from an outpatient with skin and soft tissue infection, was subjected to whole genome sequencing, antimicrobial susceptibility testing, mouse skin infection model and hemolysis analysis to identify its virulence and resistance determinants. MRSA strain SR434 is resistant to clindamycin, erythromycin and fosfomycin. Four plasmids with resistance genes were identified in this strain, including a 20,658bp blaZ-carrying plasmid, a 2473bp ermC-carrying plasmid, a 2622bp fosB7-carrying plasmid (86% identity with plasmid in a ST2590 MRSA strain) and a 4817bp lnuA-carrying plasmid (99% identity with pLNU4 from bovine coagulase-nagetive Staphylococci)...
July 13, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28733403/transformation-of-schizosaccharomyces-japonicus
#14
Keita Aoki, Hironori Niki
This protocol describes the use of electroporation to transform Schizosaccharomyces japonicus with plasmids or linear DNA. Plasmids are helpful for the complementation testing of mutations and for the expression of specific genes. Linear DNA fragments integrated into chromosomal DNA by homologous recombination are useful for gene deletion or to fuse a gene with a tag sequence (e.g., encoding a fluorescent protein). To introduce DNA into S. japonicus, electroporation methods are recommended because S. japonicus is sensitive to lithium acetate (LiOAc)...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28733249/dna-based-random-key-generation-and-management-for-otp-encryption
#15
Yunpeng Zhang, Xin Liu, Manhui Sun
One-time pad (OTP) is a principle of key generation applied to the stream ciphering method which offers total privacy. The OTP encryption scheme has proved to be unbreakable in theory, but difficult to realize in practical applications. Because OTP encryption specially requires the absolute randomness of the key, its development has suffered from dense constraints. DNA cryptography is a new and promising technology in the field of information security. DNA chromosomes storing capabilities can be used as one-time pad structures with pseudo-random number generation and indexing in order to encrypt the plaintext messages...
July 18, 2017: Bio Systems
https://www.readbyqxmd.com/read/28732914/spermine-modified-starch-based-carrier-for-gene-delivery-structure-transfection-activity-relationships
#16
Xiaoyi Huang, Xiaoxi Li, Ling Chen, Lin Li
This study aims at developing starch based gene carriers with low cytotoxicity and high transfection efficiency. Starch molecules with molecular weight of about 50kDa were cationically modified by spermine to obtain spermine modified starch (SMS) based gene carriers. Plasmid pAcGFP1-C1 (pDNA) was chosen as the model gene material and formed self-assembly nanocomplexes with SMS. The cytotoxicity and transfection efficiency of SMS/pDNA complexes were tested in HepG2 cell lines. Results showed that SMS/pDNA complexes formed by SMS-DS3 with the highest primary amine content (1...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732789/effect-of-ceftiofur-and-enrofloxacin-on-e-coli-sub-population-in-pig-gastrointestinal-tract
#17
Dachuan Lin, Kaichao Chen, Miaomiao Xie, Lianwei Ye, Edward Wai-Chi Chan, Sheng Chen
BACKGROUND: Antibiotic resistant sub-population of the gut microbiome in both human and animals may be enriched during antimicrobial treatment. METHODS: This study investigated how the size and antibiotic susceptibility profiles of antibiotic resistant sub-population organisms in the animal gastrointestinal tract changed upon exposure to different levels of ceftiofur and enrofloxacin by plating fecal samples collected from piglets before and after antibiotic treatment onto MacConkey agar containing 0...
July 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28732788/occurrence-and-clonal-diversity-of-multidrug-resistant-klebsiella-pneumoniae-recovered-from-inanimate-surfaces-in-algerian-hospital-environment-first-report-of-arma-qnrb-and-aac-6-ib-cr-genes
#18
Karima Zenati, Farida Sahli, Vincent Garcia, Sofiane Bakour, Djellali Belhadi, Jean Marc Rolain, Abdelaziz Touati
PURPOSE: The aim of this study is to characterize the molecular support of antibiotic resistance in MDR K. pneumonaie recovered from inanimate surfaces between March 2012 to February 2014 in three teaching hospitals (Setif, Bejaia and Constantine) in Algeria. RESULTS: Forty four K. pneumoniae producing ESBL were detected and blaCTX-M-15 and blaCTX-M-3 were detected respectively in 41 and 3 isolates. These K. pneumoniae isolates producing ESBL were also resistant to gentamicin (87%), tobramicin (87%), ciprofloxacin (66%) and ofloxacin (62%)...
July 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28732043/investigation-of-horizontal-gene-transfer-of-pathogenicity-islands-in-escherichia-coli-using-next-generation-sequencing
#19
Maxim Messerer, Wolfgang Fischer, Sören Schubert
Horizontal gene transfer (HGT) contributes to the evolution of bacteria. All extraintestinal pathogenic Escherichia coli (ExPEC) harbour pathogenicity islands (PAIs), however relatively little is known about the acquisition of these PAIs. Due to these islands, ExPEC have properties to colonize and invade its hosts efficiently. Even though these PAIs are known to be acquired by HGT, only very few PAIs do carry mobilization and transfer genes required for the transmission by HGT. In this study, we apply for the first time next-generation sequencing (NGS) and in silico analyses in combination with in vitro experiments to decipher the mechanisms of PAI acquisition in ExPEC...
2017: PloS One
https://www.readbyqxmd.com/read/28731685/lipophilic-polycation-vehicles-display-high-plasmid-dna-delivery-to-multiple-cell-types
#20
Yaoying Wu, Adam E Smith, Theresa M Reineke
A class of cationic poly(alkylamidoamine)s (PAAAs) containing lipophilic methylene linkers were designed and examined as in vitro plasmid DNA (pDNA) delivery agents. The PAAAs were synthesized via step-growth polymerization between a diamine monomer and each of four different diacid chloride monomers with varying methylene linker lengths, including glutaryl chloride, adipoyl chloride, pimeloyl chloride, and suberoyl chloride, which served to systematically increase the lipophilicity of the polymers. The synthesized polymers successfully complexed with pDNA in reduced serum medium at N/P ratios of 5 and greater, resulting in polyplexes with hydrodynamic diameters of approximately 1 μm...
July 21, 2017: Bioconjugate Chemistry
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