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https://www.readbyqxmd.com/read/29150514/sinorhizobium-meliloti-glutathione-reductase-is-required-for-both-redox-homeostasis-and-symbiosis
#1
Guirong Tang, Ningning Li, Yumin Liu, Liangliang Yu, Junhui Yan, Li Luo
Glutathione (L-γ-glutamyl-L-cysteinylglycine; GSH), one of the key antioxidants in Sinorhizobium meliloti, is required for the development of alfalfa (Medicago sativa) nitrogen-fixing nodules. Glutathione exists either in a reduced form (GSH) or an oxidized form (GSSG), and its content is regulated by two pathways in S. meliloti The first pathway is the de novo synthesis of glutathione from its constituent amino acids, namely, Glu, Cys, and Gly, catalyzed by γ-glutamyl cysteine synthetase (GshA) and glutathione synthetase (GshB)...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150512/a-genetic-system-for-the-thermophilic-acetogenic-bacterium-thermoanaerobacter-kivui
#2
Mirko Basen, Irina Geiger, Laura Henke, Volker Müller
Thermoanaerobacter kivui is one of the very few thermophilic acetogenic microorganisms. It grows optimally at 66°C on sugars but also lithotrophically with H2 + CO2 or with CO, producing acetate as major product. While a genome-derived model of acetogenesis has been developed, only few physiological or biochemical experiments towards the function of important enzymes in carbon and energy metabolism have been carried out. To address this issue, we developed a method for targeted, markerless gene deletions and for integration of genes into the genome of T...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150374/occurrence-of-enterobacter-hormaechei-carrying-blandm-1-and-blakpc-2-in-china
#3
Biwei Yang, Yu Feng, Alan McNally, Zhiyong Zong
Three carbapenem-resistant clinical isolates of the Enterobacter cloacae complex (ECC) were recovered from different patients in a hospital. All 3 isolates carried 2 carbapenemase genes blaKPC-2 and blaNDM-1. A study was performed to characterize their relatedness and to investigate possible links among the patients. Whole genome sequencing revealed that the isolates were Enterobacter hormaechei and belonged to ST177 of the ECC. There were 19-142 single nucleotide polymorphisms (SNPs) between the isolates, suggesting that the isolates were likely from a central reservoir, which might have existed for some time...
October 19, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29150370/emergence-of-sequence-type-252-enterobacter-cloacae-producing-ges-5-carbapenemase-in-a-czech-hospital
#4
Katerina Chudejova, Veronika Rotova, Anna Skalova, Matej Medvecky, Vaclava Adamkova, Costas C Papagiannitsis, Jaroslav Hrabak
ST252 Enterobacter cloacae, producing GES-5 carbapenemase, was isolated in a Czech hospital. blaGES-5 was part of a novel class 1 integron, In1406, which also included a new allele of the aadA15 gene cassette. In1406 was located on a ColE2-like plasmid, pEcl-35771cz (6953bp).
October 19, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29150092/generation-of-integration-free-induced-pluripotent-stem-cell-lines-derived-from-two-patients-with-x-linked-alport-syndrome-xlas
#5
Bernd Kuebler, Begoña Aran, Laia Miquel-Serra, Yolanda Muñoz, Elisabet Ars, Gemma Bullich, Monica Furlano, Roser Torra, Merce Marti, Anna Veiga, Angel Raya
Skin biopsies were obtained from two male patients with X-linked Alport syndrome (XLAS) with hemizygous COL4A5 mutations in exon 41 or exon 46. Dermal fibroblasts were extracted and reprogrammed by nucleofection with episomal plasmids carrying OCT3/4, SOX2, KLF4 LIN28, L-MYC and p53 shRNA. The generated induced Pluripotent Stem Cell (iPSC) lines AS-FiPS2-Ep6F-28 and AS-FiPS3-Ep6F-9 were free of genomically integrated reprogramming genes, had the specific mutations, a stable karyotype, expressed pluripotency markers and generated embryoid bodies which were differentiated towards the three germ layers in vitro...
September 9, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29150004/dynamics-of-indel-profiles-induced-by-various-crispr-cas9-delivery-methods
#6
Michael Kosicki, Sandeep S Rajan, Flaminia C Lorenzetti, Hans H Wandall, Yoshiki Narimatsu, Emmanouil Metzakopian, Eric P Bennett
The introduction of CRISPR/Cas9 gene editing in mammalian cells is a scientific breakthrough, which has greatly affected basic research and gene therapy. The simplicity and general access to CRISPR/Cas9 reagents has in an unprecedented manner "democratized" gene targeting in biomedical research, enabling genetic engineering of any gene in any cell, tissue, organ, and organism. The ability for fast, precise, and efficient profiling of the double-stranded break induced insertions and deletions (indels), mediated by any of the available programmable nucleases, is paramount to any given gene targeting approach...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29149242/long-term-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebsiella-pneumoniae-in-the-general-population-in-the-netherlands
#7
Engeline van Duijkeren, Cornelia C H Wielders, Cindy M Dierikx, Angela H A M van Hoek, Paul Hengeveld, Christiaan Veenman, Alice Florijn, Aniek Lotterman, Lidwien A M Smit, Jaap T van Dissel, Catharina B M Maassen, Sabine C de Greeff
Background: This longitudinal study aimed to investigate (risk factors for) persistence of carriage and molecular characteristics of extended-spectrum and pAmpC β-lactamase-producing (ESBL/pAmpC) Escherichia coli and Klebsiella pneumoniae (ESBL-E/K) in adults in the Dutch community. Methods: Following a cross-sectional study (ESBL-E/K prevalence 4.5%), a subset of ESBL-E/K positive (n=76) and negative (n=249) individuals volunteered to provide five monthly faecal samples and questionnaires...
November 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29149094/genomic-analysis-of-third-generation-cephalosporin-resistant-escherichia-coli-from-dairy-cow-manure
#8
Muhammad Attiq Rehman, Xianhua Yin, Dion Lepp, Chad Laing, Kim Ziebell, Guylaine Talbot, Edward Topp, Moussa Sory Diarra
The production of extended-spectrum β-lactamases (ESBLs) conferring resistance to new derivatives of β-lactams is a major public health threat if present in pathogenic Gram-negative bacteria. The objective of this study was to characterize ceftiofur (TIO)- or cefotaxime (FOX)-resistant Escherichia coli isolated from dairy cow manure. Twenty-four manure samples were collected from four farms and incubated under anaerobic conditions for 20 weeks at 4 °C or at 25 °C. A total of 37 TIO- or FOX-resistant E. coli were isolated from two of the four farms to determine their susceptibility to 14 antibiotics...
November 17, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29149055/exploration-for-the-salinity-tolerance-related-genes-from-xero-halophyte-atriplex-canescens-exploiting-yeast-functional-screening-system
#9
Gang Yu, Jingtao Li, Xinhua Sun, Yanzhi Liu, Xueliang Wang, Hao Zhang, Hongyu Pan
Plant productivity is limited by salinity stress, both in natural and agricultural systems. Identification of salt stress-related genes from halophyte can provide insights into mechanisms of salt stress tolerance in plants. Atriplex canescens is a xero-halophyte that exhibits optimum growth in the presence of 400 mM NaCl. A cDNA library derived from highly salt-treated A. canescens plants was constructed based on a yeast expression system. A total of 53 transgenic yeast clones expressing enhanced salt tolerance were selected from 10⁵ transformants...
November 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29148915/the-frequency-of-mutations-in-quinolone-resistance-determining-regions-and-plasmid-mediated-quinolone-resistance-in-shigella-isolates-recovered-from-pediatric-patients-in-tehran-iran-an-overlooked-problem
#10
Sajad Yaghoubi, Reza Ranjbar, Mohammad Mehdi Soltan Dallal, Mohammad Hasan Shirazi, Mohammad Kazem Sharifi-Yazdi
Fluoroquinolone (FQ) resistance in clinical isolates of Shigella species has been increasing reported in recent years. This study was carried out to find the mutations within the quinolone resistance-determining regions (QRDRs) and the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants among the clinical isolates of Shigella sp. in Tehran, Iran. A total of 50 Shigella isolates were collected from five teaching therapeutic centers in Tehran, Iran and analyzed for antibiotic susceptibility over a period of 20 months from July 2015 to January 2017...
November 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29148895/characterization-of-cefotaxime-and-ciprofloxacin-resistant-commensal-escherichia-coli-originating-from-belgian-farm-animals-indicates-high-antibiotic-resistance-transfer-rates
#11
Ellen Lambrecht, Eva Van Meervenne, Nico Boon, Tom Van de Wiele, Pierre Wattiau, Lieve Herman, Marc Heyndrickx, Els Van Coillie
Food-producing animals represent one of the sources of antibiotic resistant commensal bacteria. There is an increasing awareness that these bacteria might have the potential to transfer their resistance genes to other (pathogenic) bacteria. In this study, 50 commensal Escherichia coli strains originating from food-producing animals and resistant to the "highest priority, critically important antibiotics" cefotaxime and/or ciprofloxacin, were selected for further characterization. For each strain (i) an antibiogram, (ii) the phylogenetic group, (iii) plasmid replicon type, (iv) presence and identification of integrons, and (v) antibiotic resistance transfer ratios were determined...
November 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29148707/versatile-supermolecular-inclusion-complex-based-on-host-guest-interaction-for-targeted-gene-delivery
#12
Yunxia Sun, Jingyi Zhu, Wen-Xiu Qiu, Qi Lei, Si Chen, Xian-Zheng Zhang
A facile and targeted gene delivery system was prepared by conjugating β-cyclodextrin modified polyethylenimine (PEI-CD) and adamantyl peptide (AdGRGDS) based on host-guest interaction. With the rational design between PEI-CD and AdGRGDS, PEI-CD/AdGRGDS gene delivery system showed excellent DNA binding capability, and exhibited good ability to compact DNA into uniform spherical nanoparticles. In vitro luciferase assay showed that gene expression transfected by PEI-CD/AdGRGDS was stronger than that by PEI-CD in HeLa cells, whereas, gene expression transfected by PEI-CD/AdGRGDS and PEI-CD was similar to each other in COS7 cells...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148380/high-rate-of-mcr-1-producing-escherichia-coli-and-klebsiella-pneumoniae-among-pigs-portugal
#13
Nicolas Kieffer, Marta Aires-de-Sousa, Patrice Nordmann, Laurent Poirel
The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1-producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29147550/self-assembly-of-nucleopeptides-to-interact-with-dnas
#14
Xuewen Du, Jie Zhou, Xinming Li, Bing Xu
As a novel class of biomaterials, nucleopeptides, via the conjugation of nucleobases and peptides, usually self-assemble to form nanofibres driven mainly by hydrogen bonds. Containing nucleobase(s), nucleopeptides have a unique property-interacting with nucleic acids. Here we report the design and characterization of nucleopeptides that self-assemble in water and are able to interact with single-stranded DNAs (ssDNAs). Containing nucleobases on their side chains, these nucleopeptides bind with the ssDNAs, and the ssDNAs reciprocally affect the self-assembly of nucleopeptides...
December 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/29147487/a-study-on-the-immune-response-induced-by-a-dna-vaccine-encoding-mtb32c-hbha-antigen-of-mycobacterium-tuberculosis
#15
Roghayeh Teimourpour, Hadi Peeridogaheh, Amir Teimourpour, Mohsen Arzanlou, Zahra Meshkat
Objectives: Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. Materials and Methods: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147486/a-novel-medium-throughput-biological-assay-system-for-htlv-1-infectivity-and-drug-discovery
#16
Mojtaba Fattahi Abdizadeh, Manoochehr Makvandi, Alireza Samarbafzadeh, Kayhan Azadmanesh
Objectives: Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids. The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29146860/complete-genome-sequence-of-a-novel-nonnodulating-rhizobium-species-isolated-from-agave-americana-l-rhizosphere
#17
Víctor Manuel Ruíz-Valdiviezo, Marco Antonio Rogel-Hernandez, Gabriela Guerrero, Clara Ivette Rincón-Molina, Luis Galdino García-Perez, Federico Antonio Gutiérrez-Miceli, Juan José Villalobos-Maldonado, Aline López-López, Esperanza Martinez-Romero, Reiner Rincón-Rosales
We report here the complete genome sequence of Rhizobium sp. strain ACO-34A, isolated from Agave americana L. rhizosphere. No common nod genes were found, but there were nif genes for nitrogen fixing. A low average nucleotide identity to reported species supports its designation as a novel Rhizobium species that has a complete ribosomal operon in a plasmid.
November 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29146844/complete-genome-sequence-of-acetobacter-tropicalis-oregon-r-modencode-strain-bdgp1-an-acetic-acid-bacterium-found-in-the-drosophila-melanogaster-gut
#18
Kenneth H Wan, Charles Yu, Soo Park, Ann S Hammonds, Benjamin W Booth, Susan E Celniker
Acetobacter tropicalis Oregon-R-modENCODE strain BDGP1 was isolated from Drosophila melanogaster for functional host-microbe interaction studies. The complete genome comprises a single chromosomal circle of 3,988,649 bp with a G+C content of 56% and a conjugative plasmid of 151,013 bp.
November 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29146835/complete-genome-sequence-of-bacillus-cereus-c1l-a-plant-growth-promoting-rhizobacterium-from-the-rhizosphere-of-formosa-lily-in-taiwan
#19
Chien-Jui Huang, Po-Xing Zheng, Jheng-Yang Ou, Yao-Cheng Lin, Chao-Ying Chen
Bacillus cereus C1L, a plant growth-promoting rhizobacterium, provides protection against fungal pathogens in monocot plants. To gain new insights into the biocontrol mechanisms used by this rhizobacterium, we determined the complete genome sequence of B. cereus C1L. One chromosome and three plasmids were identified with a total size of ~6.0 Mb.
November 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29146643/are-il18rap-gene-polymorphisms-associated-with-body-mass-regulation-a-cross-sectional-study
#20
Vanesa Martínez-Barquero, Griselda de Marco, Sergio Martínez-Hervas, Victoria Adam-Felici, Cristina Pérez-Soriano, Verónica Gonzalez-Albert, Gemma Rojo, Juan Francisco Ascaso, José Tomás Real, Ana Barbara Garcia-Garcia, Juan Carlos Martín-Escudero, Raquel Cortes, Felipe Javier Chaves
OBJECTIVES: To investigate the association between IL18RAP and body mass index (BMI) and obesity and to verify the effect of a polymorphism in the microRNA136 (MIR136) IL18RAP binding region. DESIGN: We analysed samples from two Spanish cross-sectional studies, VALCAR (Spanish Mediterranean coast) and Hortega (Spanish centre). These studies aimed at analysing cardiovascular risk and development of cardiovascular disease in the general population. Both populations correspond to regions with different characteristics...
November 15, 2017: BMJ Open
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