keyword
MENU ▼
Read by QxMD icon Read
search

Cathepsin G

keyword
https://www.readbyqxmd.com/read/27913947/preclinical-evaluation-of-cathepsin-based-fluorescent-imaging-system-for-cytoreductive-surgery
#1
Carlos H F Chan, Lukas F Liesenfeld, Isabel Ferreiro-Neira, James C Cusack
BACKGROUND: Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS-HIPEC) is a treatment option for peritoneal surface malignancies. The ability to detect microscopic foci of peritoneal metastasis intraoperatively may ensure the completeness of cytoreduction. In this study, we evaluated the suitability of a hand-held cathepsin-based fluorescent imaging system for intraoperative detection of appendiceal and colorectal peritoneal metastasis. METHODS: Peritoneal tumors and normal peritoneal tissues were collected from patients with appendiceal and colorectal peritoneal metastasis...
December 2, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27913632/intracellular-activation-of-complement-3-is-responsible-for-intestinal-tissue-damage-during-mesenteric-ischemia
#2
Abhigyan Satyam, Lakshmi Kannan, Naoya Matsumoto, Mayya Geha, Peter H Lapchak, Robin Bosse, Guo-Ping Shi, Jurandir J Dalle Lucca, Maria G Tsokos, George C Tsokos
Intestinal ischemia followed by reperfusion leads to local and remote organ injury attributed to inflammatory response during the reperfusion phase. The extent to which ischemia contributes to ischemia/reperfusion injury has not been thoroughly studied. After careful evaluation of intestinal tissue following 30 min of ischemia, we noticed significant local mucosal injury in wild-type mice. This injury was drastically reduced in C3-deficient mice, suggesting C3 involvement. Depletion of circulating complement with cobra venom factor eliminated, as expected, injury recorded at the end of the reperfusion phase but failed to eliminate injury that occurred during the ischemic phase...
December 2, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27903071/reduced-levels-and-bioactivity-of-endogenous-protease-cathepsin-d-in-genital-tract-secretions-of-postmenopausal-women
#3
Mariel Jais, Naji Younes, Stacey Chapman, Susan Cu-Uvin, Mimi Ghosh
PROBLEM: HIV infections are increasing in older adult populations. Although it is known that the HIV/AIDS epidemics affect women disproportionately, little is known regarding immune responses in reproductive tract of postmenopausal women, specifically regarding endogenous proteases that modulate inflammation, tissue remodeling and wound healing. METHOD OF STUDY: Levels and bioactivity of endogenous proteases Cathepsin B, D, G, and Neutrophil Elastase were measured in cervical vaginal lavage collected from 20 HIV-negative pre- and postmenopausal women...
November 30, 2016: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/27882195/design-and-synthesis-of-tesirine-a-clinical-antibody-drug-conjugate-pyrrolobenzodiazepine-dimer-payload
#4
Arnaud C Tiberghien, Jean-Noel Levy, Luke A Masterson, Neki V Patel, Lauren R Adams, Simon Corbett, David G Williams, John A Hartley, Philip W Howard
Pyrrolobenzodiazepine dimers are an emerging class of warhead in the field of antibody-drug conjugates (ADCs). Tesirine (SG3249) was designed to combine potent antitumor activity with desirable physicochemical properties such as favorable hydrophobicity and improved conjugation characteristics. One of the reactive imines was capped with a cathepsin B-cleavable valine-alanine linker. A robust synthetic route was developed to allow the production of tesirine on clinical scale, employing a flexible, convergent strategy...
November 10, 2016: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27862990/effects-of-ferric-citrate-supplementation-on-advanced-glycation-end-products-in-a-rat-model-of-streptozotocin-nicotinamide-induced-diabetes
#5
Kuo-Ching Chao, Seu-Hwa Chen, Chun-Chao Chang, Yu-Chieh Lee, Chi-Mei Wang, Jung-Su Chang
SCOPE: Diabetes is associated with increased risks of anemia and activation of advanced glycation end products (AGEs) and the receptor for AGEs (RAGE). However, the effects of pharmacological doses of iron supplementation on AGE metabolism are less clear. The aim was to investigate the effect of ferric citrate supplementation on AGE metabolism. METHODS AND RESULTS: Diabetes was induced in overnight starved rats by intraperitoneal injections of 40 mg/kg streptozotocin and 120 mg/kg nicotinamide...
November 10, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27836038/six-year-longitudinal-study-of-fasciola-hepatica-bulk-milk-antibody-elisa-in-the-dairy-dense-region-of-the-republic-ireland
#6
M P Munita, R Rea, Y Bloemhoff, N Byrne, A M Martinez-Ibeas, R G Sayers
Completion of the F. hepatica lifecycle is dependent on suitable climatic conditions for development of immature stages of the parasite, and its snail intermediate host. Few investigations have been conducted regarding temporal variations in F. hepatica status in Irish dairy herds. The current study aimed to conduct a longitudinal study examining annual and seasonal trends in bulk milk seropositivity over six years, while also investigating associations with soil temperature, rainfall and flukicide treatment...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27806341/exogenous-cathepsin-g-upregulates-cell-surface-mhc-class-i-molecules-on-immune-and-glioblastoma-cells
#7
Madleen Giese, Nadine Turiello, Nicole Molenda, David Palesch, Annika Meid, Roman Schroeder, Paola Basilico, Charaf Benarafa, Marc-Eric Halatsch, Michal Zimecki, Mike-Andrew Westhoff, Christian Rainer Wirtz, Timo Burster
Major histocompatibility complex (MHC) class I molecules present antigenic peptides to cytotoxic T cells. During an adaptive immune response, MHC molecules are regulated by several mechanisms including lipopolysaccharide (LPS) and interferon gamma (IFN-g). However, it is unclear whether the serine protease cathepsin G (CatG), which is generally secreted by neutrophils at the site of inflammation, might regulate MHC I molecules. We identified CatG, and to a higher extend CatG and lactoferrin (LF), as an exogenous regulator of cell surface MHC I expression of immune cells and glioblastoma stem cells...
October 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27777419/lysosomal-dysfunction-in-the-brain-of-a-mouse-model-with-intraneuronal-accumulation-of-carboxyl-terminal-fragments-of-the-amyloid-precursor-protein
#8
G Kaur, M Pawlik, S E Gandy, M E Ehrlich, J F Smiley, E Levy
Recent data suggest that intraneuronal accumulation of metabolites of the amyloid-β-precursor protein (APP) is neurotoxic. We observed that transgenic mice overexpressing in neurons a human APP gene harboring the APP(E693Q) (Dutch) mutation have intraneuronal lysosomal accumulation of APP carboxylterminal fragments (APP-CTFs) and oligomeric amyloid β (oAβ) but no histological evidence of amyloid deposition. Morphometric quantification using the lysosomal marker protein 2 (LAMP-2) immunolabeling showed higher neuronal lysosomal counts in brain of 12-months-old APP(E693Q) as compared with age-matched non-transgenic littermates, and western blots showed increased lysosomal proteins including LAMP-2, cathepsin D and LC3...
October 25, 2016: Molecular Psychiatry
https://www.readbyqxmd.com/read/27762044/cathepsin%C3%A2-b-specific-metabolic-precursor-for-in%C3%A2-vivo-tumor-specific-fluorescence-imaging
#9
Man Kyu Shim, Hong Yeol Yoon, Ju Hee Ryu, Heebeom Koo, Sangmin Lee, Jae Hyung Park, Jong-Ho Kim, Seulki Lee, Martin G Pomper, Ick Chan Kwon, Kwangmeyung Kim
Recently, metabolic glycoengineering with bioorthogonal click reactions has focused on improving the tumor targeting efficiency of nanoparticles as delivery vehicles for anticancer drugs or imaging agents. It is the key technique for developing tumor-specific metabolic precursors that can generate unnatural glycans on the tumor-cell surface. A cathepsin B-specific cleavable substrate (KGRR) conjugated with triacetylated N-azidoacetyl-d-mannosamine (RR-S-Ac3 ManNAz) was developed to enable tumor cells to generate unnatural glycans that contain azide groups...
November 14, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27755915/platelet-specific-chemokines-contribute-to-the-pathogenesis-of-acute-lung-injury
#10
Khalil Bdeir, Kandace Gollomp, Marta Stasiak, Junjie Mei, Izabela Papiewska-Pajak, Guohua Zhao, G Scott Worthen, Douglas B Cines, Mortimer Poncz, M Anna Kowalska
RATIONALE: Platelets and neutrophils contribute to the development of acute lung injury (ALI). However, the mechanism by which platelets make this contribution is incompletely understood. We asked whether the two most abundant platelet chemokines, CXCL7, which induces neutrophil chemotaxis and activation, and CXCL4, which does neither, mediate ALI through complementary pathogenic pathways. OBJECTIVES: To examine the role of platelet-derived chemokines in the pathogenesis of ALI using Cxcl7-/- and Cxcl4-/- knockout mice and mice that express human CXCL7 or CXCL4...
October 18, 2016: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/27722734/polymeric-prodrug-combination-to-exploit-the-therapeutic-potential-of-antimicrobial-peptides-against-cancer-cells
#11
G J Kelly, A Foltyn-Arfa Kia, F Hassan, S O'Grady, M P Morgan, B S Creaven, S McClean, J H Harmey, M Devocelle
Antimicrobial Peptides (AMPs) have unique anticancer properties, but their clinical application is currently limited by an inadequate margin of safety. A prodrug strategy associated with a combination therapy approach could address this limitation by increasing their therapeutic index and their efficacy. Accordingly, the first targeted anticancer polymeric prodrug candidates of AMPs, intended for combination therapy with another polymeric prodrug of an approved antineoplastic agent (doxorubicin), were synthesized as either a PEG-based dual-release prodrug or two individual pegylated prodrugs...
October 4, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27716614/tnf-induced-cleavage-of-hsp90-by-cathepsin-d-potentiates-apoptotic-cell-death
#12
Jürgen Fritsch, Ricarda Fickers, Jan Klawitter, Vinzenz Särchen, Philipp Zingler, Dieter Adam, Ottmar Janssen, Eberhard Krause, Stefan Schütze
During apoptosis induction by TNF, the extrinsic and intrinsic apoptosis pathways converge at the lysosomal-mitochondrial interface. Earlier studies showed that the lysosomal aspartic protease Cathepsin D (CtsD) cleaves Bid to tBid, resulting in the amplification of the initial apoptotic cascade via mitochondrial outer membrane permeabilization (MOMP).The goal of this study was to identify further targets for CtsD that might be involved in activation upon death receptor ligation. Using a proteomics screen, we identified the heat shock protein 90 (HSP90) to be cleaved by CtsD after stimulation of U937 or other cell lines with TNF, FasL and TRAIL...
October 3, 2016: Oncotarget
https://www.readbyqxmd.com/read/27711349/caging-the-uncageable-using-metal-complex-release-for-photochemical-control-over-irreversible-inhibition
#13
Matthew Huisman, Jessica K White, Veronica G Lewalski, Izabela Podgorski, Claudia Turro, Jeremy J Kodanko
Photochemical control over irreversible inhibition was shown using Ru(ii)-caged inhibitors of cathepsin L. Levels of control were dependent on where the Ru(ii) complex was attached to the organic inhibitor, reaching >10 : 1 with optimal placement. A new strategy for photoreleasing Ru(ii) fragments from inhibitor-enzyme conjugates is also reported.
October 18, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27676391/the-role-of-neutrophil-proteins-on-the-amyloid-beta-rage-axis
#14
Amanda J Stock, Anne Kasus-Jacobi, Jonathan D Wren, Virginie H Sjoelund, Glenn D Prestwich, H Anne Pereira
We previously showed an elevated expression of the neutrophil protein, cationic antimicrobial protein of 37kDa (CAP37), in brains of patients with Alzheimer's disease (AD), suggesting that CAP37 could be involved in AD pathogenesis. The first step in determining how CAP37 might contribute to AD pathogenesis was to identify the receptor through which it induces cell responses. To identify a putative receptor, we performed GAMMA analysis to determine genes that positively correlated with CAP37 in terms of expression...
2016: PloS One
https://www.readbyqxmd.com/read/27671822/boswellic-acids-and-their-role-in-chronic-inflammatory-diseases
#15
H P T Ammon
: Boswellic acids, which are pentacyclic triterpenes belong to the active pharmacological compounds of the oleogum resin of different Boswellia species. In the resin, more than 12 different boswellic acids have been identified but only KBA and AKBA received significant pharmacological interest. Biological Activity: In an extract of the resin of Boswellia species multiple factors are responsible for the final outcome of a therapeutic effect, be it synergistic or antagonistic. Moreover, the anti-inflammatory actions of BAs are caused by different mechanisms of action...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27666013/cell-surface-cathepsin-g-activity-differs-between-human-natural-killer-cell-subsets
#16
Adriane Penczek, Marcin Sienczyk, Christian Rainer Wirtz, Timo Burster
Natural killer (NK) cells are critical in diverse defense mechanisms, including elimination of viral infected cells and destruction of tumor cells. NK cells are characterized by the ability to initiate apoptosis in target cells when their cell surface major histocompatibility complex class I (MHC I) repertoire is missing. On the other hand, NK cells are not activated when MHC I or non-classical MHC molecules are found on the respective cells. It was demonstrated that cathepsin G (CatG) binds to the cell surface of NK cells; however, the distribution of this protease on the cell surface of NK cell subsets has not been identified...
September 24, 2016: Immunology Letters
https://www.readbyqxmd.com/read/27660294/cathepsin-g-controls-arterial-but-not-venular-myeloid-cell-recruitment
#17
Almudena Ortega Gomez, Melanie Salvermoser, Jan Rossaint, Robert Pick, Janine Brauner, Patricia Lemnitzer, Jessica Tilgner, Renske de Jong, Remco T A Megens, Janina Jamasbi, Yvonne Döring, Christine T Pham, Christoph Scheiermann, Wolfgang Siess, Maik Drechsler, Christian Weber, Jochen Grommes, Alexander Zarbock, Barbara Walzog, Oliver Soehnlein
BACKGROUND: -Therapeutic targeting of arterial leukocyte recruitment in the context of atherosclerosis has been disappointing in clinical studies. Reasons for such failures include the lack of knowledge of arterial-specific recruitment patterns. Here we establish the importance of the Cathepsin G (CatG) in the context of arterial myeloid cell recruitment. METHODS: -Intravital microscopy of the carotid artery, the jugular vein and cremasteric arterioles and venules in Apoe(-/-) and CatG-deficient mice Apoe(-/-)Ctsg(-/-)) mice was employed to study site-specific myeloid cell behavior after high fat diet (HFD) feeding or TNF stimulation...
September 22, 2016: Circulation
https://www.readbyqxmd.com/read/27651029/amniotic-fluid-cathepsin-g-in-pregnancies-complicated-by-the-preterm-prelabor-rupture-of-membranes
#18
Ivana Musilova, Ctirad Andrys, Marcela Drahosova, Ondrej Soucek, Lenka Pliskova, Martin Stepan, Tomas Bestvina, Jan Maly, Bo Jacobsson, Marian Kacerovsky
OBJECTIVE: The aim of this study was to evaluate the amniotic fluid cathepsin-G concentrations in women with preterm prelabor rupture of membranes (PPROM) based on the presence of the microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: A total of 154 women with singleton pregnancies complicated by PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid cathepsin-G concentrations were assessed by ELISA...
October 9, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27650250/effects-of-high-epa-and-high-dha-fish-oils-on-changes-in-signaling-associated-with-protein-metabolism-induced-by-hindlimb-suspension-in-rats
#19
Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Luís Gustavo De Sousa, Gilson M Murata, Amanda Rabello Crisma, Carlos Flores Rodrigues Junior, Phablo Abreu, Rosângela Pavan Torres, Jorge Mancini-Filho, Sandro M Hirabara, Philip Newsholme, Rui Curi
The effects of either eicosapentaenoic (EPA)- or docosahexaenoic (DHA)-rich fish oils on hindlimb suspension (HS)-induced muscle disuse atrophy were compared. Daily oral supplementations (0.3 mL/100 g b.w.) with mineral oil (MO) or high EPA or high DHA fish oils were performed in adult rats. After 2 weeks, the animals were subjected to HS for further 2 weeks. The treatments were maintained alongside HS At the end of 4 weeks, we evaluated: body weight gain, muscle mass and fat depots, composition of fatty acids, cross-sectional areas (CSA) of the soleus muscle and soleus muscle fibers, activities of cathepsin L and 26S proteasome, and content of carbonylated proteins in the soleus muscle...
September 2016: Physiological Reports
https://www.readbyqxmd.com/read/27648120/cathepsin-b-regulates-collagen-expression-by-fibroblasts-via-prolonging-tlr2-nf-%C3%AE%C2%BAb-activation
#20
Xue Li, Zhou Wu, Junjun Ni, Yicong Liu, Jie Meng, Weixian Yu, Hiroshi Nakanishi, Yanmin Zhou
Fibroblasts are essential for tissue repair due to producing collagens, and lysosomal proteinase cathepsin B (CatB) is involved in promoting chronic inflammation. We herein report that CatB regulates the expression of collagens III and IV by fibroblasts in response to a TLR2 agonist, lipopolysaccharide from Porphyromonas gingivalis (P.g. LPS). In cultured human BJ fibroblasts, mRNA expression of CatB was significantly increased, while that of collagens III and IV was significantly decreased at 24 h after challenge with P...
2016: Oxidative Medicine and Cellular Longevity
keyword
keyword
97862
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"