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https://www.readbyqxmd.com/read/28484981/human-health-risk-assessment-due-to-dietary-intake-of-heavy-metals-through-rice-in-the-mining-areas-of-singhbhum-copper-belt-india
#1
Soma Giri, Abhay Kumar Singh
The study was intended to investigate heavy metal contamination levels in the rice grown in the vicinity of the mining areas of Singhbhum Copper Belt, India. The concentrations of the metals were below the Indian maximum allowable concentrations for food except for Pb, Ni, and Zn at some locations. Principal component analysis extracted three factors explaining 79.1% of the data variability. The extracted factors suggested that the sources of metals in the rice can be attributed to soil, irrigating water, and atmospheric dust deposition...
May 9, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28480907/metabolomic-analysis-of-the-effects-of-cadmium-and-copper-treatment-in-oryza-sativa-l-using-untargeted-liquid-chromatography-coupled-to-high-resolution-mass-spectrometry-and-all-ion-fragmentation
#2
Meritxell Navarro-Reig, Joaquim Jaumot, Benjamín Piña, Encarnación Moyano, Maria Teresa Galceran, Romà Tauler
While the knowledge of plant metabolomes has increased in the last few years, their response to the presence of toxicants is still poorly understood. Here, we analyse the metabolomic changes in Japanese rice (Oryza sativa var. Japonica) upon exposure to heavy metals (Cd(ii) and Cu(ii)) in concentrations from 10 to 1000 μM. After harvesting, rice metabolites were extracted from aerial parts of the plants and analysed by HPLC (HILIC TSK gel amide-80 column) coupled to a mass spectrometer quadrupole-Orbitrap (Q-Exactive)...
May 8, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28461082/mechanism-study-of-sulfur-fertilization-mediating-copper-translocation-and-biotransformation-in-rice-oryza-sativa-l-plants
#3
Lijuan Sun, Jianjun Yang, Huaxiang Fang, Chen Xu, Cheng Peng, Haomin Huang, Lingli Lu, Dechao Duan, Xiangzhi Zhang, Jiyan Shi
Metabolism of sulfur (S) is suggested to be an important factor for the homeostasis and detoxification of Cu in plants. We investigated the effects of S fertilizers (S(0), Na2SO4) on Cu translocation and biotransformation in rice plants by using multiple synchrotron-based techniques. Fertilization of S increased the biomass and yield of rice plants, as well as the translocation factor of Cu from root to shoot and shoot to grain, resulting in enhanced Cu in grain. Sulfur K-edge X-ray near edge structure (XANES) analysis showed that fertilization of S increased the concentration of glutathione in different rice tissues, especially in rice stem and leaf...
April 28, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28391850/increasing-the-efficiency-of-immobilization-and-chitin-determination-using-copper-oxide-nanoparticles
#4
Preety, Vinita Hooda
A novel polyurethane (PU) support with and without copper oxide nanoparticles (nCuO) was employed for immobilization of two chitinolytic enzymes, chitinase and N-acetyl β glucosaminidase (NAGase) to yield PU/nCuO/chitinase/NAGase and PU/chitinase/NAGase conjugates respectively. The surface morphologies, topologies and bonded interactions between different components of the immobilized enzyme conjugates were characterized using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Fourier Transform Infrared Spectroscopy (FTIR) respectively...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28303048/studies-on-physico-chemical-and-cooking-characteristics-of-rice-bean-varieties-grown-in-ne-region-of-india
#5
Rejaul Hoque Bepary, D D Wadikar, Seuji Borah Neog, P E Patki
Rice bean (Vigna umbellata) is grown in South and Southeast Asia, and the bean has gained importance due to its nutritional strength in terms of dietary fiber, quality protein and minerals. In current study, the nutritional and functional components, cooking and thermo-gravimetric properties of eleven rice bean varieties from NE India were investigated. Results revealed that the major nutrients among the varieties ranged as follows: 54.21-60.49% carbohydrates, 15.64-21.60% protein, 1.22-2.3% fat, 5.53-6.56% crude fibre, 3...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28289984/in-silico-characterization-and-transcriptomic-analysis-of-nif-family-genes-from-anabaena-sp-pcc7120
#6
Shilpi Singh, Alok Kumar Shrivastava
In silico approaches in conjunction with morphology, nitrogenase activity, and qRT-PCR explore the impact of selected abiotic stressor such as arsenic, salt, cadmium, copper, and butachlor on nitrogen fixing (nif family) genes of diazotrophic cyanobacterium Anabaena sp. PCC7120. A total of 19 nif genes are present within the Anabaena genome that is involved in the process of nitrogen fixation. Docking studies revealed the interaction between these nif gene-encoded proteins and the selected abiotic stressors which were further validated through decreased heterocyst frequency, fragmentation of filaments, and downregulation of nitrogenase activity under these stresses indicating towards their toxic impact on nitrogen fixation potential of filamentous cyanobacterium Anabaena sp...
March 14, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28146098/comprehensive-analysis-of-rice-laccase-gene-oslac-family-and-ectopic-expression-of-oslac10-enhances-tolerance-to-copper-stress-in-arabidopsis
#7
Qingquan Liu, Le Luo, Xiaoxiao Wang, Zhenguo Shen, Luqing Zheng
Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h...
January 30, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28129387/mutation-in-mg-protoporphyrin-ix-monomethyl-ester-cyclase-decreases-photosynthesis-capacity-in-rice
#8
Xuexia Wang, Rongfeng Huang, Ruidang Quan
In photosynthesis, the pigments chlorophyll a/b absorb light energy to convert to chemical energy in chloroplasts. Though most enzymes of chlorophyll biosynthesis from glutamyl-tRNA to chlorophyll a/b have been identified, the exact composition and regulation of the multimeric enzyme Mg-protoporphyrin IX monomethyl ester cyclase (MPEC) is largely unknown. In this study, we isolated a rice pale-green leaf mutant m167 with yellow-green leaf phenotype across the whole lifespan. Chlorophyll content decreases 43-51% and the granal stacks of chloroplasts becomes thinner in m167...
2017: PloS One
https://www.readbyqxmd.com/read/28036003/dep-on-go-for-simultaneous-sensing-of-multiple-heavy-metals-pollutants-in-environmental-samples
#9
Madhu Biyani, Radhika Biyani, Tomoko Tsuchihashi, Yuzuru Takamura, Hiromi Ushijima, Eiichi Tamiya, Manish Biyani
We describe a simple and affordable "Disposable electrode printed (DEP)-On-Go" sensing platform for the rapid on-site monitoring of trace heavy metal pollutants in environmental samples for early warning by developing a mobile electrochemical device composed of palm-sized potentiostat and disposable unmodified screen-printed electrode chips. We present the analytical performance of our device for the sensitive detection of major heavy metal ions, namely, mercury, cadmium, lead, arsenic, zinc, and copper with detection limits of 1...
December 27, 2016: Sensors
https://www.readbyqxmd.com/read/27987672/antibacterial-activity-of-agricultural-waste-derived-wollastonite-doped-with-copper-for-bone-tissue-engineering
#10
S Azeena, N Subhapradha, N Selvamurugan, S Narayan, N Srinivasan, R Murugesan, T W Chung, A Moorthi
Bioactive ceramic materials with metal ions generation brought great attention in the class of biomaterials development and widely employed as a filler material for bone tissue regeneration. The present study aimed to fabricate calcium silicate based ceramic material doped with copper metal particles by sol-gel method. Rice straw of agricultural waste was utilized as a source material to synthesize wollastonite, then wollastonite was doped with copper to fabricate copper doped wollastonite (Cu-Ws) particles...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27855895/dispersive-liquid-liquid-microextraction-based-on-solidification-of-floating-organic-drop-for-preconcentration-and-determination-of-trace-amounts-of-copper-by-flame-atomic-absorption-spectrometry
#11
Cennet Karadaş, Derya Kara
A novel, simple, rapid, sensitive, inexpensive and environmentally friendly dispersive liquid-liquid microextraction method based on the solidification of a floating organic drop (DLLME-SFO) was developed for the determination of copper by flame atomic absorption spectrometry (FAAS). N-o-Vanillidine-2-amino-p-cresol was used as a chelating ligand and 1-undecanol was selected as an extraction solvent. The main parameters affecting the performance of DLLME-SFO, such as sample pH, volume of extraction solvent, extraction time, concentration of the chelating ligand, salt effect, centrifugation time and sample volume were investigated and optimized...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27853967/the-bioconcentration-ability-of-heavy-metal-research-for-50-kinds-of-rice-under-the-same-test-conditions
#12
Wen-Juan Xie, Lei Che, Guang-Yu Zhou, Li-Na Yang, Min-Yu Hu
The aims of this experiment are to explore the accumulation of metal contamination of different varieties of rice planted in paddy fields and to provide a basis for the further research. The rice specimens were grown in and collected from a total area of 8.24 acres of rice planting fields where local farmers cultivated 50 different kinds of rice. The crops were grown using the methods of seedling, transplanting, fertilizing, and irrigation, under the guidance of professional and technical personnel. The 50 kinds of paddy rice contain 20 kinds of conventional rice, 15 kinds of two-line hybrid rice, 15 kinds of three-line hybrid rice, and the whole experiment lasted 100 days...
December 2016: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27833841/plant-growth-promotion-and-biofortification-of-chickpea-and-pigeonpea-through-inoculation-of-biocontrol-potential-bacteria-isolated-from-organic-soils
#13
Subramaniam Gopalakrishnan, Srinivas Vadlamudi, Srinivasan Samineni, C V Sameer Kumar
Seven strains of bacteria [Pseudomonas plecoglossicida SRI-156, Brevibacterium antiquum SRI-158, Bacillus altitudinis SRI-178, Enterobacter ludwigii SRI-211, E. ludwigii SRI-229, Acinetobacter tandoii SRI-305 and Pseudomonas monteilii SRI-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (PGP) in rice] were evaluated for their PGP and biofortification traits in chickpea and pigeonpea under field conditions. When treated on seed, the seven selected bacteria significantly enhanced the shoot height and root length of both chickpea and pigeonpea over the un-inoculated control...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27822471/isolation-and-physicochemical-characterization-of-laccase-from-ganoderma-lucidum-cdbt1-isolated-from-its-native-habitat-in-nepal
#14
Prabin Shrestha, Bishnu Joshi, Jarina Joshi, Rajani Malla, Lakshmaiah Sreerama
At present, few organisms are known to and capable of naturally producing laccases and white rot fungi are one such group. In the present study, three fungal species, namely, Ganoderma lucidum-CDBT1, Ganoderma japonicum, and Lentinula edodes, isolated from their native habitat in Nepal were screened for laccase production, and G. lucidum-CDBT1 was found to express highest levels of enzyme (day 10 culture media showed 0.92 IU/mg total protein or 92 IU/mL laccase activity with ABTS as substrate). Lignin extracted from rice straw was used in Olga medium for laccase production and isolation from G...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27786355/effect-of-sulfur-containing-spices-on-the-bioaccessibility-of-trace-minerals-from-selected-cereals-and-pulses
#15
Meena Kumari, Kalpana Platel
BACKGROUND: Garlic and onion, which are rich in organo-sulfur compounds, are reported to enhance the bioaccessibility of Ca, Mg, Fe and Zn; however, there is a lack of similar information on the bioaccessibility of copper, manganese and chromium. Therefore, the present study aimed to determine the effect of exogenous garlic and onion on the bioaccessibility of these trace minerals from selected food grains. The effect of two levels of garlic (0.25 and 0.5 g/10 g grain(-1) ) and onion (1...
October 27, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27746792/genome-wide-identification-and-evolution-analysis-of-trehalose-6-phosphate-synthase-gene-family-in-nelumbo-nucifera
#16
Qijiang Jin, Xin Hu, Xin Li, Bei Wang, Yanjie Wang, Hongwei Jiang, Neil Mattson, Yingchun Xu
Trehalose-6-phosphate synthase (TPS) plays a key role in plant carbohydrate metabolism and the perception of carbohydrate availability. In the present work, the publicly available Nelumbo nucifera (lotus) genome sequence database was analyzed which led to identification of nine lotus TPS genes (NnTPS). It was found that at least two introns are included in the coding sequences of NnTPS genes. When the motif compositions were analyzed we found that NnTPS generally shared the similar motifs, implying that they have similar functions...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27733860/association-of-increased-grain-iron-and-zinc-concentrations-with-agro-morphological-traits-of-biofortified-rice
#17
Laura T Moreno-Moyano, Julien P Bonneau, José T Sánchez-Palacios, Joseph Tohme, Alexander A T Johnson
Biofortification of rice (Oryza sativa L.) with micronutrients is widely recognized as a sustainable strategy to alleviate human iron (Fe) and zinc (Zn) deficiencies in developing countries where rice is the staple food. Constitutive overexpression of the rice nicotianamine synthase (OsNAS) genes has been successfully implemented to increase Fe and Zn concentrations in unpolished and polished rice grain. Intensive research is now needed to couple this high-micronutrient trait with high grain yields. We investigated associations of increased grain Fe and Zn concentrations with agro-morphological traits of backcross twice second filial (BC2F2) transgenic progeny carrying OsNAS1 or OsNAS2 overexpression constructs under indica/japonica and japonica/japonica genetic backgrounds...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27713011/the-influence-of-metal-ions-on-the-dielectric-enhancement-and-radical-generation-of-rice-starch-during-microwave-processing
#18
Da-Ming Fan, Lu-Fen Lin, Li-Yun Wang, Lue-Lue Huang, Bo Hu, Xiao-Hong Gu, Jian-Xin Zhao, Hao Zhang
In this study, the effect of the enrichment of rice starch with metal ions on its capability to generate free radicals during microwave processing was investigated. The underlying mechanism was explored from two aspects: dielectric and thermal properties. Rice starch was modified with ions of iron, copper, manganese and calcium. The modified starches were analyzed in terms of dielectric properties, activation energy (Ea) and thermodynamic characteristics of gelatinization. The quantity and character of the free radicals were analyzed using electron paramagnetic resonance (EPR)...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27636863/migration-of-copper-from-nanocopper-ldpe-composite-films
#19
Fang Liu, Chang-Ying Hu, Quan Zhao, Yu-Jie Shi, Huai-Ning Zhong
Three nanocopper/low-density polyethylene (LDPE) composite films were tested in food simulants (3% acetic acid and 10% ethanol) and real food matrices (rice vinegar, bottled water and Chinese liquor) to explore the behaviours of copper migration using ICP-OES and GFAAS. The effects of exposure time, temperature, nanocopper concentration and contact media on the release of copper from nanocopper/LDPE composite films were studied. It was shown that the migration of copper into 10% ethanol was much less than that into 3% acetic acid at the same conditions...
November 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27633505/the-expression-of-heterologous-fe-iii-phytosiderophore-transporter-hvys1-in-rice-increases-fe-uptake-translocation-and-seed-loading-and-excludes-heavy-metals-by-selective-fe-transport
#20
Raviraj Banakar, Ána Alvarez Fernández, Javier Abadía, Teresa Capell, Paul Christou
Many metal transporters in plants are promiscuous, accommodating multiple divalent cations including some which are toxic to humans. Previous attempts to increase the iron (Fe) and zinc (Zn) content of rice endosperm by overexpressing different metal transporters have therefore led unintentionally to the accumulation of copper (Cu), manganese (Mn) and cadmium (Cd). Unlike other metal transporters, barley Yellow Stripe 1 (HvYS1) is specific for Fe. We investigated the mechanistic basis of this preference by constitutively expressing HvYS1 in rice under the control of the maize ubiquitin1 promoter and comparing the mobilization and loading of different metals...
April 2017: Plant Biotechnology Journal
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