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https://www.readbyqxmd.com/read/29785534/an-enzyme-free-homogenous-electrochemical-assay-for-sensitive-detection-of-the-plasmid-mediated-colistin-resistance-gene-mcr-1
#1
Bo Li, Zhixin Chai, Xiaohui Yan, Chunchen Liu, Bo Situ, Ye Zhang, Weilun Pan, Shihua Luo, Jianhua Liu, Lei Zheng
Antibiotic resistance associated with the mcr-1 gene of Gram-negative bacteria, which confers resistance to drugs of last resort and has the potential to spread via plasmids, is one of the most pressing issues facing global health today. Point-of-care testing for the mcr-1 gene is needed to aid in the identification of colistin resistance in the field and to control its horizontal transmission. Here, we report the successful development of an enzyme-free homogenous electrochemical strategy for sensitive detection of the antibiotic resistance gene mcr-1 using the hybridization chain reaction and mcr-1-specific toehold probe...
May 22, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29784391/exploiting-the-synergistic-effect-of-concurrent-data-signals-low-level-fusion-of-liquid-chromatographic-with-dual-detection-data
#2
Carla M Teglia, Silvana M Azcarate, Mirta R Alcaráz, Héctor C Goicoechea, María J Culzoni
A low-level data fusion strategy was developed and implemented for data processing of second-order liquid chromatographic data with dual detection, i.e. absorbance and fluorescence monitoring. The synergistic effect of coupling individual information provided by two different detectors was evaluated by analyzing the results gathered after the application of a series of data preprocessing steps and chemometric resolution. The chemometric modeling involved data analysis by MCR-ALS, PARAFAC and N-PLS. Their ability to handle the new data block was assessed through the estimation of the analytical figures of merits achieved in the prediction of a validation set containing fifteen fluorescent and non-fluorescent veterinary active ingredients that can be found in poultry litter...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783148/comparative-study-of-six-sequential-spectrophotometric-methods-for-quantification-and-separation-of-ribavirin-sofosbuvir-and-daclatasvir-an-application-on-laboratory-prepared-mixture-pharmaceutical-preparations-spiked-human-urine-spiked-human-plasma-and-dissolution
#3
Wafaa S Hassan, Manal S Elmasry, Heba M Elsayed, Dalia W Zidan
In accordance with International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines, six novel, simple and precise sequential spectrophotometric methods were developed and validated for the simultaneous analysis of Ribavirin (RIB), Sofosbuvir (SOF), and Daclatasvir (DAC) in their mixture without prior separation steps. These drugs are described as co-administered for treatment of Hepatitis C virus (HCV). HCV is the cause of hepatitis C and some cancers such as liver cancer (hepatocellular carcinoma) and lymphomas in humans...
May 18, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29782210/colonization-with-enterobacteriaceae-producing-esbls-ampcs-and-oxa-48-in-wild-avian-species-spain-2015-2016
#4
Jesús Oteo, Aida Mencía, Verónica Bautista, Natalia Pastor, Noelia Lara, Fernando González-González, Francisco Javier García-Peña, José Campos
Antibiotic resistance is a global threat of complex and changeable epidemiology. The role of wild birds in the dissemination of antibacterial resistance might be underestimated. We studied the cloacal colonization by cefotaxime-resistant Enterobacteriaceae in 668 wild birds in Spain. Eighty-eight wild birds (13.2%) of 28 species carried cefotaxime-resistant isolates; 58 of them (8.7%) carried extended-spectrum β-lactamases (ESBLs) and 15 (2.5%) plasmid-mediated AmpCs of the blaCIT family. The 58 ESBLs belonged to the CTX-M-1 group (63...
May 21, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29779959/considering-disability-and-health-reflections-on-the-healthy-people-2020-midcourse-review
#5
Lisa Bundara Sinclair, Michael H Fox, Bruce S Jonas, Hugh G Berry, Louis A Quatrano, Angela K McGowan, Georgina Peacock
The Healthy People 2020 (HP2020) Midcourse Review (MCR) presents an opportunity for professionals in the disability and health field to contemplate preliminary progress toward achieving specific health objectives. The MCR showed notable progress in access to primary care, appropriate services for complex conditions associated with disability, expansion of health promotion programs focusing on disability, improving mental health, and reducing the unemployment rate among job seekers with disabilities. This commentary presents potential considerations, at least in part, for such progress including increased access to health care, greater awareness of appropriate services for complex conditions, and opportunities for societal participation...
April 19, 2018: Disability and Health Journal
https://www.readbyqxmd.com/read/29778190/emergence-of-the-colistin-resistance-gene-mcr-1-and-its-variant-in-several-uncommon-species-of-enterobacteriaceae-from-commercial-poultry-farm-surrounding-environments
#6
Xiaoming Wang, Yao Wang, Yang Wang, Suxia Zhang, Zhangqi Shen, Shaolin Wang
The colistin resistance gene mcr-1 has been detected in multiple members of Enterobacteriaceae family. Here, we report the emergence of mcr-1 in Providencia alcalifaciens and a mcr-1 variant, named mcr-1.3, in Raoultella planticola. Both of the mcr-1-carrying plasmids in these two isolates belong to IncI2 type of plasmids, but they are different in sizes and genetic characteristics. We also detected the mcr-1 gene in one Enterobacter cloacae isolate, however, the mcr-1-carrying plasmid is distinct from the previous reports...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778043/investigation-of-interactions-of-comtan-with-human-serum-albumin-by-mathematically-modeled-voltammetric-data-a-study-from-bio-interaction-to-biosensing
#7
Ali R Jalalvand, Sirous Ghobadi, Hector C Goicoechea, Hui-Wen Gu, Esmael Sanchooli
In this work, voltammetric data recorded at a glassy carbon electrode (GCE) were separately used to investigate the interactions of entacapone (Comtan, CAT) with human serum albumin (HSA). Then, an augmented data matrix was constructed by the combination of voltammetric and spectroscopic data and simultaneously analysed by multivariate curve resolution-alternating least squares (MCR-ALS) to obtain more information about CAT-HSA interactions. The absence of rotational ambiguities in results obtained by MCR-ALS was verified with the help of MCR-BANDS and we confirmed that the results were unambiguous and reliable...
May 16, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29774212/alkaline-peptone-water-based-enrichment-method-for-mcr-3-from-acute-diarrheic-outpatient-gut-samples
#8
Qiaoling Sun, Yanyan Hu, Hongwei Zhou, Lingbin Shu, Hanyu Wang, Zixian Huang, Rong Zhang
A third plasmid-mediated colistin resistance gene, mcr-3 , is increasingly being reported in Enterobacteriaceae and Aeromonas spp. from animals and humans. To investigate the molecular epidemiology of mcr in the gut flora of Chinese outpatients, 152 stool specimens were randomly collected from outpatients in our hospital from May to June, 2017. Stool specimens enriched in alkaline peptone water or Luria-Bertani (LB) broth were screened for mcr-1, mcr-2 , and mcr-3 using polymerase chain reaction (PCR)-based assays...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29768099/the-mcr-1-gene-has-no-effect-on-colistin-resistance-when-it-coexists-with-inactivated-mgrb-gene-in-klebsiella-pneumoniae
#9
Huichuan Zhang, Dongdong Zhao, Qiucheng Shi, Jingjing Quan, Xi Li, Yunsong Yu
The inactivated mgrB gene and the mcr-1 gene are important mechanisms of colistin resistance in Klebsiella pneumoniae and they are threats to the clinical use of colistin. In this study, mcr-1 gene was cloned into K. pneumoniae strains (XH209 and KP10) and their derived strains (XH209 M and KP10 M), which showed high-level resistance to colistin. The acquisition of the mcr-1 gene led to colistin resistance in XH209 and KP10, but the addition of mcr-1 gene did not cause change of colistin minimum inhibitory concentrations in the XH209 M and KP10 M...
May 16, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29760138/co-location-of-the-polymyxin-resistance-gene-mcr-1-and-variant-of-mcr-3-on-a-plasmid-in-escherichia-coli-from-chicken-farm
#10
Rong Xiang, Bi-Hui Liu, An-Yun Zhang, Chang-Wei Lei, Xiao-Lan Ye, Yan-Xian Yang, Yan-Peng Chen, Hong-Ning Wang
A colistin-resistant Escherichia coli from commercial poultry farm in China carried two colistin-resistance genes mcr-1 and variant of mcr-3 in an IncP plasmid. The variant of mcr-3 gene, named mcr-3.11 , encoded two amino acid substitutions compared with the mcr-3 A novel genetic structure, IS Kpn40-mcr-3-dgkA - IS Kpn40 , might be the key element mediating translocation of mcr-3 through the formation of circular form. The mcr-1 and mcr-3 genes co-located on a plasmid might pose huge threat to public health...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29758020/defining-icr-mo-an-intrinsic-colistin-resistance-determinant-from-moraxella-osloensis
#11
Wenhui Wei, Swaminath Srinivas, Jingxia Lin, Zichen Tang, Shihua Wang, Saif Ullah, Vishnu Goutham Kota, Youjun Feng
Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family...
May 14, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29756143/potential-use-of-mcr-als-for-the-identification-of-coeliac-related-biochemical-changes-in-hyperspectral-raman-maps-from-pediatric-intestinal-biopsies
#12
Stefano Fornasaro, Annalisa Vicario, Luigina De Leo, Alois Bonifacio, Tarcisio Not, Valter Sergo
Raman hyperspectral imaging is an emerging practice in biological and biomedical research for label free analysis of tissues and cells. Using this method, both spatial distribution and spectral information of analyzed samples can be obtained. The current study reports the first Raman microspectroscopic characterisation of colon tissues from patients with Coeliac Disease (CD). The aim was to assess if Raman imaging coupled with hyperspectral multivariate image analysis is capable of detecting the alterations in the biochemical composition of intestinal tissues associated with CD...
May 14, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29755416/complete-genomic-analysis-of-a-salmonella-enterica-serovar-typhimurium-isolate-cultured-from-ready-to-eat-pork-in-china-carrying-one-large-plasmid-containing-mcr-1
#13
Wei Wang, Zulqarnain Baloch, Mingyuan Zou, Yinping Dong, Zixin Peng, Yujie Hu, Jin Xu, Nafeesa Yasmeen, Fengqin Li, Séamus Fanning
One mcr-1 -carrying ST34-type Salmonella Typhimurium WW012 was cultured from 3,200 ready-to-eat (RTE) pork samples in 2014 in China. Broth dilution method was applied to obtain the antimicrobial susceptibility of Salmonella Typhimurium WW012. Broth matting assays were carried out to detect transferability of this phenotype and whole-genome sequencing was performed to analyze its genomic characteristic. Thirty out of 3,200 RTE samples were positive for Salmonella and the three most frequent serotypes were identified as S ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29749233/quantifying-the-prediction-error-in-analytical-multivariate-curve-resolution-studies-of-multicomponent-systems
#14
Rocio B Pellegrino Vidal, Alejandro Cesar Olivieri, Romà Tauler
In multivariate curve resolution (MCR) analysis a range of feasible solutions is often encountered, due to the rotational ambiguities associated to the bilinear decomposition of data matrices. For quantitative purposes, the analysis is usually applied to a carefully designed set of calibration and test samples having uncalibrated interferents. Under the usual minimal constraints (non-negativity, unimodality, species correspondence, etc.), concentration and spectral profiles of the analyte in the test samples are not univocally recovered, unlike those in the test samples, especially when profile overlapping with the interferents is significant and selective regions do not exist for the analyte...
May 11, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29743535/identification-of-a-unique-radical-sam-methyltransferase-required-for-the-sp-3-c-methylation-of-an-arginine-residue-of-methyl-coenzyme-m-reductase
#15
Darja Deobald, Lorenz Adrian, Christian Schöne, Michael Rother, Gunhild Layer
The biological formation of methane (methanogenesis) is a globally important process, which is exploited in biogas technology, but also contributes to global warming through the release of a potent greenhouse gas into the atmosphere. The last and methane-releasing step of methanogenesis is catalysed by the enzyme methyl-coenzyme M reductase (MCR), which carries several exceptional posttranslational amino acid modifications. Among these, a 5-C-(S)-methylarginine is located close to the active site of the enzyme...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29741287/longitudinal-study-on-the-occurrence-in-pigs-of-colistin-resistant-e-coli-carrying-mcr-1-following-the-cessation-of-use-of-colistin
#16
Luke P Randall, Robert A Horton, Fabrizio Lemma, Francesca Martelli, Nick A D Duggett, Richard P Smith, Miranda J Kirchner, Richard J Ellis, Jon P Rogers, Susanna M Williamson, Robin R L Simons, Camilla M Brena, Sarah J Evans, Muna F Anjum, Christopher J Teale
AIMS: In 2015, colistin-resistant Escherichia coli and Salmonella with the mcr-1 gene were isolated from a pig farm in Great Britain. Pigs were subsequently monitored over a ~ 20 month period for the occurrence of mcr-1 mediated colistin resistance and the risk of mcr-1 E. coli entering the food chain was assessed. METHODS AND RESULTS: Pig faeces and slurry were cultured for colistin resistant E. coli and Salmonella, tested for the mcr-1 gene by PCR, and selected isolates were further analysed...
May 9, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29731740/evidence-for-environmental-dissemination-of-antibiotic-resistance-mediated-by-wild-birds
#17
Jiao Wu, Ye Huang, Dawei Rao, Yongkui Zhang, Kun Yang
The aquatic bird, egret, could carry antibiotic resistance (AR) from a contaminated waterway (Jin River, Chengdu, China) into the surrounding environment (Wangjianglou Park). A systematic study was carried out on the unique environmental dissemination mode of AR mediated by birds. The minimum inhibitory concentrations of various antibiotics against the environmental Escherichia coli isolates were used to evaluate the bacterial AR at the environmental locations where these isolates were recovered, i.e., the Jin River water, the egret feces, the park soil, and the campus soil...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29731646/in-vitro-antibacterial-effect-of-fosfomycin-combination-therapy-against-colistin-resistant-klebsiella-pneumoniae
#18
Wei Yu, Qixia Luo, Qingyi Shi, Chen Huang, Xiao Yu, Tianshui Niu, Kai Zhou, Jiajie Zhang, Yonghong Xiao
Objectives: Colistin is still a "last-resort" antibiotic used to manage human infections due to multidrug-resistant (MDR) Klebsiella pneumoniae . However, colistin-resistant K. pneumoniae (CR-Kp) isolates emerged a decade ago and had a worldwide distribution. The purpose of this study was to evaluate the genetic data of CR-Kp and identify the antibacterial activity of fosfomycin (FM) alone and in combination with amikacin (AMK) or colistin (COL) against CR-Kp in vitro. Methods: Three clinical CR-Kp isolates from three patients were collected...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29730781/role-of-antiplatelet-therapy-in-the-durability-of-hemodialysis-access
#19
Satinderjit Locham, Robert J Beaulieu, Hanaa Dakour-Aridi, Besma Nejim, Mahmoud B Malas
BACKGROUND: Antiplatelet therapy (APT) is often used on anecdotal grounds to improve vascular access patency. The aim of this study was to assess the role of APT in hemodialysis (HD) patients undergoing arteriovenous fistula (AVF) or graft (AVG) placement. METHODS: All patients in a large HD vascular qualitative initiative database (2011-2017) were included and divided into no antiplatelet therapy (no-APT) vs. any APT [aspirin (ASA) or P2Y12 inhibitors (PI)]. Multivariate [logistic (MLR) and Cox (MCR) regression] analyses were used as appropriate...
May 5, 2018: Journal of Nephrology
https://www.readbyqxmd.com/read/29729080/discovery-of-benzimidazole-based-l-mexicana-cysteine-protease-cpb2-8%C3%AE-cte-inhibitors-as-potential-therapeutics-for-leishmaniasis
#20
Laura De Luca, Stefania Ferro, Maria Rosa Buemi, Anna-Maria Monforte, Rosaria Gitto, Tanja Schirmeister, Louis Maes, Antonio Rescifina, Nicola Micale
Chemotherapy is currently the only effective approach to treat all forms of leishmaniasis. However, its effectiveness is severely limited due to high toxicity, long treatment length, drug-resistance or inadequate mode of administration. As a consequence, there is a need to identify new molecular scaffolds and targets as potential therapeutics for the treatment of this disease. We report a small series of 1,2-substituted-1H-benzo[d]imidazole derivatives (9a-d) showing affinity in the submicromolar range (Ki = 0...
May 4, 2018: Chemical Biology & Drug Design
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