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https://www.readbyqxmd.com/read/28938480/dysregulation-of-the-anti-m%C3%A3-llerian-hormone-system-by-steroids-in-women-with-polycystic-ovary-syndrome
#1
Alice Pierre, Joëlle Taieb, Frank Giton, Michaël Grynberg, Salma Touleimat, Hady El Hachem, Renato Fanchin, Danielle Monniaux, Joëlle Cohen-Tannoudji, Nathalie di Clemente, Chrystèle Racine
Context: Anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR2) are overexpressed in granulosa cells (GCs) from women with polycystic ovary syndrome (PCOS), the most common cause of female infertility. Objective: The aim of the study was to compare the regulation of the AMH/AMHR2 system by 5α-dihydrotestosterone (5α-DHT) and estradiol (E2) in GCs from control and PCOS women. Design, Setting, Patients: Experiments were performed on follicular fluids (FF) and GCs from women undergoing in vitro fertilization...
August 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938177/the-osmotin-of-calotropis-procera-latex-is-not-expressed-in-laticifer-free-cultivated-callus-and-under-salt-stress
#2
Isabel C C Souza, Márcio V Ramos, José H Costa, Cleverson D T Freitas, Raquel S B Oliveira, Frederico B Moreno, Renato A Moreira, Cristina P S Carvalho
The latex of Calotropis procera has previously been reported to contain osmotin. This protein (CpOsm) inhibited phytopathogens and this was mechanistically characterized. Here, the time-course profile of CpOsm transcripts was examined in the salt-stressed cultivated callus of C. procera in order to better understand its role in the physiology of the plant. Stressed callus (80 mM NaCl) showed an unbalanced content of organic compounds (proline and total soluble sugar) and inorganic ions (Na(+), Cl(-), and K(+))...
September 14, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28937879/advax4-delta-inulin-combination-adjuvant-together-with-ecmx-a-fusion-construct-of-three-protective-mtb-antigens-induces-a-potent-th1-immune-response-and-protects-mice-against-mycobacterium-tuberculosis-infection
#3
Bruno de Paula Oliveira Santos, Monalisa Martins Trentini, Renato Beilner Machado, Mara Rúbia Nunes Celes, André Kipnis, Nikolai Petrovsky, Ana Paula Junqueira-Kipnis
Tuberculosis (TB) remains a main public health concern and 10.4 million new cases occurred in 2015 around the world. BCG is the only approved vaccine against TB, but has variable efficacy and new vaccines are needed. We developed two new mTB vaccine candidates based on the recombinant fusion proteins, rCMX and rECMXformulated with Advax4, a new combination adjuvant combining delta inulin, CpG oligonucleotide and murabutide. When BALB/c mice were immunized three times intramuscularly with these vaccine formulationsInjection of Advax4 alone increased the percentage of lymphatic endothelial cells and activated macrophages (F480/CD11b(+)) in the draining lymph nodes consistent with a chemotactic adjuvant effect...
September 22, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28935429/glycemic-control-in-patients-undergoing-coronary-artery-bypass-graft-surgery-clinical-features-predictors-and-outcomes
#4
Judson B Williams, Eric D Peterson, Álvaro S Albrecht, Shuang Li, Sameer A Hirji, T Ferguson, Peter K Smith, Renato D Lopes
PURPOSE: Critically ill patients with hyperglycemia have worse prognosis. The degree to which glycemic control is achieved following CABG surgery and the association with clinical outcomes is not well understood. MATERIALS AND METHODS: We studied patients undergoing higher risk CABG surgery at 55 US hospitals. Good glycemic control was defined as 70-180mg/dL in the first 24h postoperatively. Generalized estimating equations logistic regression models were used to assess the relationship between glycemic control and clinical outcomes after adjusting for baseline characteristics...
September 9, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28934279/spontaneous-non-canonical-assembly-of-ccmk-hexameric-components-from-%C3%AE-carboxysome-shells-of-cyanobacteria
#5
Luis F Garcia-Alles, Eric Lesniewska, Katharina Root, Nathalie Aubry, Nicolas Pocholle, Carlos I Mendoza, Eric Bourillot, Konstantin Barylyuk, Denis Pompon, Renato Zenobi, David Reguera, Gilles Truan
CcmK proteins are major constituents of icosahedral shells of β-carboxysomes, a bacterial microcompartment that plays a key role for CO2 fixation in nature. Supported by the characterization of bidimensional (2D) layers of packed CcmK hexamers in crystal and electron microscopy structures, CcmK are assumed to be the major components of icosahedral flat facets. Here, we reassessed the validity of this model by studying CcmK isoforms from Synechocystis sp. PCC6803. Native mass spectrometry studies confirmed that CcmK are hexamers in solution...
2017: PloS One
https://www.readbyqxmd.com/read/28933764/thermographic-evaluation-of-the-hands-of-pig-slaughterhouse-workers-exposed-to-cold-temperatures
#6
Adriana Seára Tirloni, Diogo Cunha Dos Reis, Eliane Ramos, Antônio Renato Pereira Moro
Brazil was rated the fourth leading producer and exporter of pork meat in the world. The aim of this study was to evaluate the temperature of the hands of pig slaughterhouse workers and its relation to the thermal sensation of the hands and the use of a cutting tool. The study included 106 workers in a pig slaughterhouse. An infrared camera FlirThermaCAM E320 (Flir Systems, Wilsonville, OR, USA) was used to collect the images of the dorsal and palmar surfaces of both hands. A numerical scale was used to obtain the thermal sensation...
July 26, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28933515/b-cell-lymphoblastic-lymphoma-presenting-as-solitary-temporal-mass-with-amplification-of-aml1-runx1-case-report
#7
Rocco Sabatino, Gabriella Aquino, Antonio Pinto, Miguel Angel Piris, Laura Marra, Maria Napolitano, Annarosaria De Chiara, Renato Franco
No abstract text is available yet for this article.
September 2017: Hematological Oncology
https://www.readbyqxmd.com/read/28932707/neisseria-gonorrhoeae-challenge-increases-matrix-metalloproteinase-8-expression-in-fallopian-tube-explants
#8
Natalia E Juica, Paula I Rodas, Paula Solar, Paula Borda, Renato Vargas, Cristobal Muñoz, Rodolfo Paredes, Myron Christodoulides, Luis A Velasquez
Background:Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28932334/corneal-biomechanics-in-ectatic-diseases-refractive-surgery-implications
#9
Renato Ambrósio, Fernando Faria Correia, Bernardo Lopes, Marcella Q Salomão, Allan Luz, Daniel G Dawson, Ahmed Elsheikh, Riccardo Vinciguerra, Paolo Vinciguerra, Cynthia J Roberts
BACKGROUND: Ectasia development occurs due to a chronic corneal biomechanical decompensation or weakness, resulting in stromal thinning and corneal protrusion. This leads to corneal steepening, increase in astigmatism, and irregularity. In corneal refractive surgery, the detection of mild forms of ectasia pre-operatively is essential to avoid post-operative progressive ectasia, which also depends on the impact of the procedure on the cornea. METHOD: The advent of 3D tomography is proven as a significant advancement to further characterize corneal shape beyond front surface topography, which is still relevant...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28930522/advances-in-tick-vaccinology-in-brazil-from-gene-expression-to-immunoprotection
#10
Renato Andreotti, Poliana Fernanda Giachetto, Rodrigo Casquero Cunha
Rhipicephalus (Boophilus) microplus has substantial economic impact on the cattle breeding industry and, chemical control and tick resistance development are the major concern. There is a worldwide search for new options, and control using vaccines has been the main focus nowadays. Studies performed in Brazil found that Bm86-based immunization of bovines reduced the infestation of R. (B.) microplus of vaccinated bovines by 45% to 60%. Native Boophilusmicroplus tripsin inhibitors (BmTIs) with trypsin-, kallikrein-, and elastase-inhibiting activities have been used as immunogens in bovines reaching 72...
January 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28930274/the-efficiency-of-noni-morinda-citrifolia-l-essential-oil-on-the-control-of-leaf-spot-caused-by-exserohilum-turcicum-in-maize-culture
#11
Janaina Costa E Silva, Dalmarcia de Sousa Carlos Mourão, Fabia Silva de Oliveira Lima, Renato de Almeida Sarmento, Mateus Sunti Dalcin, Raimundo Wagner de Souza Aguiar, Gil Rodrigues Dos Santos
The objective of this work was to evaluate the efficiency of noni essential oil on the control of Exserohilum turcicum, a causative agent of Exserohilum spot in maize culture. In the sanitary test 400 seeds were incubated using the blotter test method. For the transmissibility test, the fragments of damaged leaves of seedlings were removed and put into a potato, dextrose and agar (PDA) culture environment. To verify the pathogenicity, Koch´s postulates were performed. In the phytotoxicity test different concentrations of noni oil were applied in maize seedlings...
August 14, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28926870/cardiovascular-responses-to-resistance-exercise-performed-with-large-and-small-muscle-mass
#12
Lenifran Matos-Santos, Paulo Farinatti, Juliana P Borges, Renato Massaferri, Walace Monteiro
Prior research about the effects of the amount of exercised muscle mass upon cardiovascular responses (CVR) has neglected a potential bias related to total exercise and concentric/eccentric duration. Autonomic responses and perceived exertion (RPE) were compared in resistance exercises performed with larger and smaller muscle mass and matched for total exercise and concentric/eccentric duration. Twelve men performed 4 sets of 12 repetitions of unilateral (UNI) and bilateral (BIL) knee extensions at 70% of 12RM...
September 19, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28926591/no-evidence-for-the-presence-of-epstein-barr-virus-in-squamous-cell-carcinoma-of-the-mobile-tongue
#13
Torben Wilms, Gulfaraz Khan, Philip J Coates, Nicola Sgaramella, Robin Fåhraeus, Asma Hassani, Pretty S Philip, Lena Norberg Spaak, Luigi Califano, Giuseppe Colella, Katarina Olofsson, Christos Loizou, Renato Franco, Karin Nylander
Squamous cell carcinoma of the head and neck (SCCHN) comprises a large group of cancers in the oral cavity and nasopharyngeal area that typically arise in older males in association with alcohol/tobacco usage. Within the oral cavity, the mobile tongue is the most common site for tumour development. The incidence of tongue squamous cell carcinoma (TSCC) is increasing in younger people, which has been suggested to associate with a viral aetiology. Two common human oncogenic viruses, human papilloma virus (HPV) and Epstein-Barr virus (EBV) are known causes of certain types of SCCHN, namely the oropharynx and nasopharynx, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28925862/biofactories-for-the-production-of-recombinant-phytases-and-its-application-in-animal-feed-industry
#14
Thais P P de Souza, Resyla M da Mariano, Mariana S Vieira, Suliana F V Andrade, Renato R Godoi, Ana Flavia A Gonçalves, Luciana P Naves, William J N Lima, Daniel B Gonçalves, Mariana Campos da Paz, Alexsandro S Galdino
BACKGROUND: Phytases are enzymes capable of degrading phytic acid and are used in animal feed supplementation in order to improve digestibility through the release of minerals such as phosphorus. Recent inventions show interest in production and optimization of recombinant phytases with biochemical and physicochemical characteristics promising for animal feed industry. METHODS: This review article is focused on relevant patents of the promising phytases, as well as the commonly used expression systems for its production, and also the tools currently used to generate new phytases...
September 15, 2017: Recent Patents on Biotechnology
https://www.readbyqxmd.com/read/28923966/amazon-plant-diversity-revealed-by-a-taxonomically-verified-species-list
#15
Domingos Cardoso, Tiina Särkinen, Sara Alexander, André M Amorim, Volker Bittrich, Marcela Celis, Douglas C Daly, Pedro Fiaschi, Vicki A Funk, Leandro L Giacomin, Renato Goldenberg, Gustavo Heiden, João Iganci, Carol L Kelloff, Sandra Knapp, Haroldo Cavalcante de Lima, Anderson F P Machado, Rubens Manoel Dos Santos, Renato Mello-Silva, Fabián A Michelangeli, John Mitchell, Peter Moonlight, Pedro Luís Rodrigues de Moraes, Scott A Mori, Teonildes Sacramento Nunes, Terry D Pennington, José Rubens Pirani, Ghillean T Prance, Luciano Paganucci de Queiroz, Alessandro Rapini, Ricarda Riina, Carlos Alberto Vargas Rincon, Nádia Roque, Gustavo Shimizu, Marcos Sobral, João Renato Stehmann, Warren D Stevens, Charlotte M Taylor, Marcelo Trovó, Cássio van den Berg, Henk van der Werff, Pedro Lage Viana, Charles E Zartman, Rafaela Campostrini Forzza
Recent debates on the number of plant species in the vast lowland rain forests of the Amazon have been based largely on model estimates, neglecting published checklists based on verified voucher data. Here we collate taxonomically verified checklists to present a list of seed plant species from lowland Amazon rain forests. Our list comprises 14,003 species, of which 6,727 are trees. These figures are similar to estimates derived from nonparametric ecological models, but they contrast strongly with predictions of much higher tree diversity derived from parametric models...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28922539/structural-characterization-of-a-covalent-monolayer-sheet-obtained-by-two-dimensional-polymerization-at-an-air-water-interface
#16
Vivian Müller, Feng Shao, Milos Baljozovic, Mina Moradi, Yao Zhang, Thomas Jung, William B Thompson, Benjamin T King, Renato Zenobi, A Dieter Schlüter
This work describes a two-dimensional polymerization at an air/water interface and provides, for the first time, direct spectroscopic evidence for the kind of cross-links formed and for the conversion reached in a covalently bonded monolayer sheet. This is achieved by a combination of a variety of monolayer characterization techniques before and after transfer onto solid substrates in particular by tip-enhanced Raman spectroscopy (TERS) and TERS mapping after transfer of both the monomer and polymer monolayer onto Au(111)...
September 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28921685/four-year-incidence-rate-and-predictors-of-root-caries-among-community-dwelling-south-brazilian-older-adults
#17
Augusto B Bidinotto, Aline B Martins, Camila M Dos Santos, Fernando N Hugo, Juliana B Hilgert, Roger Keller Celeste, Dalva Maria Padilha, Renato José De Marchi
OBJECTIVE: This study aimed to assess the incidence density (ID) of new root caries lesions and restorations among community-living south Brazilian older adults and to assess its association with predictor variables. METHODS: We conducted a prospective cohort with a random sample of elders from the municipality of Carlos Barbosa, in south Brazil. Trained dentists performed interviews and oral examinations in 388 individuals at baseline, of which 273 were retained at follow-up 4 years later...
September 18, 2017: Community Dentistry and Oral Epidemiology
https://www.readbyqxmd.com/read/28921430/production-of-recombinant-rabies-virus-glycoprotein-by-insect-cells-in-a-single-use-fixed-bed-bioreactor
#18
Daniella C Ventini-Monteiro, Renato M Astray, Carlos A Pereira
A single-use fixed-bed bioreactor (iCELLis nano) can be used for cultivating non adherent insect cells, which can be then recovered for scaling up or for harvesting a membrane-associated viral glycoprotein with high quality in terms of preserved protein structure and biological function. Here, we describe the procedures for establishing genetically modified Drosophila melanogaster Schneider 2 (S2) cell cultures in the iCELLis nano bioreactor and for quantifying by ELISA the recombinant rabies virus glycoprotein (rRVGP) synthesized...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28918569/highlights-from-the-ninth-international-symposium-of-thrombosis-and-anticoagulation-ista-ix-october-15-2016-salvador-bahia-brazil
#19
REVIEW
Renato D Lopes, Patricia O Guimarães, Elaine Hylek, Gilson S Feitosa-Filho, Luiz Ritt, Nivaldo Filgueiras, Eduardo Darzé, Mario S Rocha, Luis P Magalhães, Antonio Carlos Sobral Sousa, Luis Claudio Correia, Lucas Hollanda Oliveira, David A Garcia
To discuss and share knowledge about advances in the care of patients with thrombotic disorders, the Ninth International Symposium of Thrombosis and Anticoagulation was held in Salvador, Bahia, Brazil, on October 15, 2016. This scientific program was developed by clinicians for clinicians and was promoted by two major clinical research institutes-the Brazilian Clinical Research Institute and the Duke Clinical Research Institute of the Duke University School of Medicine. Comprising academic presentations and open discussion, the symposium had as its primary goal to educate, motivate, and inspire internists, cardiologists, hematologists, and other physicians by convening national and international visionaries, thought-leaders, and dedicated clinician-scientists...
September 16, 2017: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/28918504/autism-spectrum-disorders-and-disease-modeling-using-stem-cells
#20
REVIEW
Anita Brito, Fabiele Baldino Russo, Alysson Renato Muotri, Patricia Cristina Baleeiro Beltrão-Braga
Autism spectrum disorders (ASD) represent a variety of disorders characterized as complex lifelong neurodevelopment disabilities, which may affect the ability of communication and socialization, including typical comportments like repetitive and stereotyped behavior. Other comorbidities are usually present, such as echolalia, hypotonia, intellectual disability and difficulties in processing figured speech. Furthermore, some ASD individuals may present certain abilities, such as eidetic memory, outstanding musical or painting talents and special mathematical skills, among others...
September 16, 2017: Cell and Tissue Research
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