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https://www.readbyqxmd.com/read/28504349/polysome-profiling-of-mab-producing-cho-cell-lines-links-translational-control-of-cell-proliferation-and-recombinant-mrna-loading-onto-ribosomes-with-global-and-recombinant-protein-synthesis
#1
Charlotte L Godfrey, Emma J Mead, Olalekan Daramola, Sarah Dunn, Diane Hatton, Ray Field, Gary Pettman, C Mark Smales
mRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to reprogramming of translation and decreased global protein synthesis. Here we apply polysome profiling to determine reprogramming and translational capabilities in host and recombinant monoclonal antibody-producing (mAb) CHO cell lines during batch culture. Recombinant cell lines with the fastest cell specific growth rates were those with the highest global translational efficiency...
May 15, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28500977/endocytosis-and-intracellular-traffic-of-cholesterol-pdmaema-liposome-complexes-in-human-epithelial-like-cells
#2
F Szymanowski, A A Hugo, P Alves, P N Simões, A Gómez-Zavaglia, Pablo F Pérez
Liposomes are generally used as delivery systems, as they are capable of encapsulating a wide variety of molecules (i.e. plasmids, recombinant proteins, therapeutic drugs). However, liposomal drug delivery have to fulfill different requirements, such as the effective internalization by the target cells and avoidance of the degradative activity of the intracellular compartments. The use of polymer lipid complexes (PLCs), by including different polymers in the liposome formulation, could improve internalization and intracellular release of drugs...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28498800/locally-applied-5-fluorouracil-loaded-slow-release-patch-prevents-pancreatic-cancer-growth-in-an-orthotopic-mouse-model
#3
In Kyong Shim, Hye-Jin Yi, Hee-Gyeong Yi, Chan Mi Lee, Yu Na Lee, Yeong-Jin Choi, Seong-Yun Jeong, Eunsung Jun, Robert M Hoffman, Dong-Woo Cho, Song Cheol Kim
To obtain improved efficacy against pancreatic cancer, we investigated the efficacy and safety of a locally-applied 5-fluorouracil (5-FU)-loaded polymeric patch on pancreatic tumors in an orthotopic nude-mouse model. The 5-FU-releasing polymeric patch was produced by 3D printing. After application of the patch, it released the drug slowly for 4 weeks, and suppressed BxPC-3 pancreas cancer growth. Luciferase imaging of BxPC3-Luc cells implanted in the pancreas was performed longitudinally. The drug patch delivered a 30...
April 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28494339/application-of-protein-a-modified-capillary-channeled-polymer-polypropylene-fibers-to-the-quantitation-of-igg-in-complex-matrices
#4
Hung K Trang, R Kenneth Marcus
Polypropylene (PP) capillary-channeled polymer (C-CP) fibers loaded with recombinant Staphyloccocus aureus protein A (rSPA) were used as an affinity chromatography stationary phase for the quantitation of immunoglobulin G (IgG) in complex biological matrices. Optimization of the chromatographic method regarding mobile phase components and load/elution conditions was performed. The six-minute analysis, including a load step with 12mM phosphate at pH 7.4, an elution step with 0.025% phosphoric acid and a re-equilibration step, was employed for quantitation of IgG1 from 0...
April 22, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28492492/the-effect-of-dietary-glycemic-properties-on-markers-of-inflammation-insulin-resistance-and-body-composition-in-postmenopausal-american-women-an-ancillary-study-from-a-multicenter-protein-supplementation-trial
#5
Violeta Stojkovic, Christine A Simpson, Rebecca R Sullivan, Anna Maria Cusano, Jane E Kerstetter, Anne M Kenny, Karl L Insogna, Jessica D Bihuniak
Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance. We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population...
May 11, 2017: Nutrients
https://www.readbyqxmd.com/read/28492302/metal-organic-framework-templated-catalysts-dual-sensitization-of-pdo-zno-composite-on-hollow-sno2-nanotubes-for-selective-acetone-sensors
#6
Won-Tae Koo, Ji-Soo Jang, Seon-Jin Choi, Hee-Jin Cho, Il-Doo Kim
Metal-organic framework (MOF) derived synergistic catalysts were easily functionalized on hollow SnO2 nanotubes (NTs) via electrospinning and subsequent calcination. Nanoscale Pd NPs (~ 2 nm) loaded Zn based zeolite imidazole framework (Pd@ZIF-8, ~ 80 nm) was used as a new catalyst loading platform for effective functionalization of PdO@ZnO complex catalyst onto the thin wall of one-dimensional (1D) metal oxide NTs. The well-dispersed nanoscale PdO catalysts (3¬-4 nm) and multi-heterojunctions (PdO/ZnO and ZnO/SnO2) on hollow structures are essential for the development of high-performance gas sensors...
May 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28483700/chemosensitizing-indomethacin-conjugated-chitosan-oligosaccharide-nanoparticles-for-tumor-targeted-drug-delivery
#7
Jae-Young Lee, Ubonvan Termsarasab, Mee Yeon Lee, Dong-Hwan Kim, Song Yi Lee, Jung Sun Kim, Hyun-Jong Cho, Dae-Duk Kim
A chitosan oligosaccharide (CSO)-indomethacin (IDM) conjugate (CI) was synthesized to fabricate chemosensitizing nanoparticles (NPs) for tumor-targeted drug delivery. IDM was conjugated to a CSO backbone via amide bond formation, of which successful synthesis was confirmed by proton-nuclear magnetic resonance analyses. Doxorubicin (DOX)-loaded CI (CI10/DOX; CI:DOX = 10:1 [w/w]) NPs with < 75 nm of mean diameter, polydispersity index of ∼0.2, and positive zeta potential were prepared. The release of DOX from the NPs was enhanced at acidic pH (pH 5...
May 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28470059/enhancement-of-pool-boiling-heat-transfer-using-aligned-silicon-nanowire-arrays
#8
Dong Il Shim, Geehong Choi, Namkyu Lee, Taehwan Kim, Beom Seok Kim, Hyung Hee Cho
Enhancing the critical heat flux (CHF), which is the capacity of heat dissipation, is important to secure high stability in two-phase cooling systems. Coolant supply to a dry hot spot is a major mechanism to prevent surface burn-out for enhancing the CHF. Here, we demonstrate a more ready supply of coolant using aligned silicon nanowires (A-SiNWs), with a high aspect ratio (>10) compared to that of conventional random silicon nanowires (R-SiNWs), which have a disordered arrangement, for additional CHF improvement...
May 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28457207/early-and-marked-enhancement-of-new-bone-quality-by-alendronate-loaded-collagen-sponge-combined-with-bone-morphogenetic-protein-2-at-high-dose-a-long-term-study-in-calvarial-defects-in-a-rat-model
#9
Tae Hyung Cho, In Sook Kim, Beomseok Lee, Si-Nae Park, Jae-Hyung Ko, Soon Jung Hwang
Choice of appropriate biomaterial is a key factor for the success of recombinant human bone morphogenetic protein (rhBMP)-2 therapy. Inspired by the osteogenic cell differentiating and osteoclast suppressing capabilities of alendronate (ALN), we manufactured a composite type of ALN-loaded collagen sponge (ALN-CS) which controls the early detrimental effect of high-dose rhBMP-2. This study aimed to evaluate ALN-CS as a high-dose rhBMP-2 carrier by investigating its initial bio-molecular effect and efficacy on intramembranous ossification at 1, 4, 8 and 24 weeks using a rat calvarial defect model, compared with non-loaded CS...
April 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28445077/effect-of-epiretinal-membranes-on-antivascular-endothelial-growth-factor-treatment-for-neovascular-age-related-macular-degeneration
#10
Han Joo Cho, Jae Min Kim, Hyoung Seok Kim, Dong Won Lee, Chul Gu Kim, Jong Woo Kim
PURPOSE: To evaluate the effect of epiretinal membranes (ERMs), detected with spectral-domain optical coherence tomography (SD-OCT), on the outcome of antivascular endothelial growth factor (VEGF) treatment for neovascular age-related macular degeneration (nAMD). METHODS: A total of 434 eyes with treatment-naive nAMD were retrospectively included and analyzed. All patients were administered an initial series of 3 monthly loading injections of ranibizumab or aflibercept, followed by further injections as required...
April 26, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28444785/liquid-crystalline-elastomers-with-gold-nanoparticle-cross-linkers
#11
Michał Wójcik, Jaroslaw Wrobel, Zuzanna Janczuk, Jozef Mieczkowski, Ewa Gorecka, Joonmyung Choi, Maenghyo Cho, Damian Pociecha
Embedding nanoparticles in a responsive polymer matrix is a formidable way to fabricate hybrid materials with predesigned properties and prospective applications in actuators, mechanically tunable optical elements or electroclinic films. However, achieving chemical compatibility between nanoparticles and organic matter is not trivial and often results in disordered structures. Herein we show that using nanoparticles as exclusive cross-linkers in the preparation of liquid-crystalline polymers can yield long-range ordered liquid-crystalline elastomers with high loading of well dispersed nanoparticles, as confirmed with small angle XRD measurements...
April 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28427403/preoperative-carbohydrate-load-and-intraoperatively-infused-omega-3-polyunsaturated-fatty-acids-positively-impact-nosocomial-morbidity-after-coronary-artery-bypass-grafting-a-double-blind-controlled-randomized-trial
#12
Gibran Roder Feguri, Paulo Ruiz Lúcio de Lima, Danilo de Cerqueira Borges, Laura Ramos Toledo, Larissa Nadaf Batista, Thaís Carvalho E Silva, Neuber José Segri, José Eduardo de Aguilar-Nascimento
BACKGROUND: A strategy of limited preoperative fasting, with carbohydrate (CHO) loading and intraoperative infusion of omega-3 polyunsaturated fatty acids (ω-3 PUFA), has seldom been tried in cardiovascular surgery. Brief fasting, followed by CHO intake 2 h before anesthesia, may improve recovery from CABG procedures and lower perioperative vasoactive drug requirements. Infusion of ω-3 PUFA may reduce occurrences of postoperative atrial fibrillation (POAF) and shorten hospital stays...
April 20, 2017: Nutrition Journal
https://www.readbyqxmd.com/read/28423731/a-multifunctional-lipid-nanoparticle-for-co-delivery-of-paclitaxel-and-curcumin-for-targeted-delivery-and-enhanced-cytotoxicity-in-multidrug-resistant-breast-cancer-cells
#13
Jong-Suep Baek, Cheong-Weon Cho
The objective of the work was to develop a multifunctional nanomedicine based on a folate-conjugated lipid nanoparticles loaded with paclitaxel and curcumin. The novel system combines therapeutic advantageous of efficient targeted delivery via folate and timed-release of curcumin and paclitaxel via 2-hydroxypropyl-ß-cyclodextrin, thereby overcoming multidrug resistance in breast cancer cells (MCF-7/ADR). The faster release of curcumin from the folate-conjugated curcumin and paclitaxel-loaded lipid nanoparticles enables sufficient p-glycoprotein inhibition, which allows increased cellular uptake and cytotoxicity of paclitaxel...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422506/extra-large-pore-mesoporous-silica-nanoparticles-for-directing-in-vivo-m2-macrophage-polarization-by-delivering-il-4
#14
Dohyeong Kwon, Bong Geun Cha, Yuri Cho, Jiyoun Min, Eun-Byeol Park, Suk-Jo Kang, Jaeyun Kim
Over the past decade, mesoporous silica nanoparticles (MSNs) smaller than 200 nm with a high colloidal stability have been extensively studied for systemic drug delivery. Although small molecule delivery via MSNs has been successful, the encapsulation of large therapeutic biomolecules, such as proteins or DNA, is limited due to small pore size of the conventional MSNs obtained by soft-templating. Here, we report the synthesis of mesoporous silica nanoparticles with extra-large pores (XL-MSNs) and their application to in vivo cytokine delivery for macrophage polarization...
April 24, 2017: Nano Letters
https://www.readbyqxmd.com/read/28415029/multifunctional-nanoparticles-self-assembled-from-polyethylenimine-based-graft-polymers-as-efficient-anticancer-drug-delivery
#15
Jianqin Yan, Ting Su, Furong Cheng, Jun Cao, Hai Zhang, Bin He
Multiple functionalization of nanoparticles has attracted great interest in drug delivery. In this paper, polymeric amphiphiles of polyethylenimine (PEI) conjugated with methoxy poly(ethylene glycol) aldehyde (mPEG-CHO), poly(ε caprolactone) aldehyde (PCL-CHO) and pyrene-1-carboxaldehyde (Py-CHO) were synthesized via Schiff's reaction. The conjugates self-assembled into nanoparticles with pH-sensitivity to load anticancer drug doxorubicin (DOX), further coated with hyaluronic acid (HA) for tumor targeting...
February 24, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28414137/novel-dabigatran-etexilate-hemisuccinate-loaded-polycap-physicochemical-characterisation-and-in-vivo-evaluation-in-beagle-dogs
#16
Jung Hyun Cho, Jin Cheul Kim, Hyung-Seo Kim, Dong Shik Kim, Kyeong Soo Kim, Yong Il Kim, Chul Soon Yong, Jong Oh Kim, Yu Seok Youn, Kyung Taek Oh, Jong Soo Woo, Han-Gon Choi
The purpose of this study was to develop a novel dabigatran etexilate hemisuccinate (DEH) salt-loaded polycap with bioequivalence to the dabigatran etexilate mesylate (DEM)-loaded commercial product. DEH prepared with dabigatran etexilate base (DE) and succinic acid was less hygroscopic but less soluble than DEM. Numerous micronized DEHs and DEH-loaded solid dispersions were prepared employing the spiral jet-milling and spray-drying techniques, respectively. Among the formulations prepared, a micronized DEH prepared with the injection air at 1...
April 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28412471/surface-modification-of-solid-lipid-nanoparticles-for-oral-delivery-of-curcumin-improvement-of-bioavailability-through-enhanced-cellular-uptake-and-lymphatic-uptake
#17
Jong-Suep Baek, Cheong-Weon Cho
Curcumin has been reported to exhibit potent anticancer effects. However, poor solubility, bioavailability and stability of curcumin limit its in vivo efficacy for the cancer treatment. Solid lipid nanoparticles (SLN) are a promising delivery system for the enhancement of bioavailability of hydrophobic drugs. However, burst release of drug from SLN in acidic environment limits its usage as oral delivery system. Hence, we prepared N-carboxymethyl chitosan (NCC) coated curcumin-loaded SLN (NCC-SLN) to inhibit the rapid release of curcumin in acidic environment and enhance the bioavailability...
April 12, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28412223/bioengineered-robust-hybrid-hydrogels-enrich-the-stability-and-efficacy-of-biological-drugs
#18
Moon Soo Gil, Jinhwan Cho, Thavasyappan Thambi, V H Giang Phan, Inchan Kwon, Doo Sung Lee
Biological drugs are exquisitely tailored components offering the advantages of high specificity and efficacy that are considered safe for treating diseases. Nevertheless, the effectiveness of biological drugs is limited by their inherent short biological half-life and poor stability in vivo. Herein, we engineered a novel delivery platform based on hybrid injectable hydrogels, in which pH- and temperature-responsive biodegradable copolymers were site-specifically coupled to the sulfhydryl group of human serum albumin, which effectively enhances the stability and circulation half-life of the biological drug, recombinant uricase enzyme (Uox)...
April 12, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28408827/photothermal-modulated-drug-delivery-and-magnetic-relaxation-based-on-collagen-poly-%C3%AE-glutamic-acid-hydrogel
#19
Sun-Hee Cho, Ahreum Kim, Woojung Shin, Min Beom Heo, Hyun Jong Noh, Kwan Soo Hong, Jee-Hyun Cho, Yong Taik Lim
Injectable and stimuli-responsive hydrogels have attracted attention in molecular imaging and drug delivery because encapsulated diagnostic or therapeutic components in the hydrogel can be used to image or change the microenvironment of the injection site by controlling various stimuli such as enzymes, temperature, pH, and photonic energy. In this study, we developed a novel injectable and photoresponsive composite hydrogel composed of anticancer drugs, imaging contrast agents, bio-derived collagen, and multifaceted anionic polypeptide, poly (γ-glutamic acid) (γ-PGA)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28408648/azithromycin-causes-a-novel-proarrhythmic-syndrome
#20
Zhenjiang Yang, Joseph K Prinsen, Kevin R Bersell, Wangzhen Shen, Liudmila Yermalitskaya, Tatiana Sidorova, Paula B Luis, Lynn Hall, Wei Zhang, Liping Du, Ginger Milne, Patrick Tucker, Alfred L George, Courtney M Campbell, Robert A Pickett, Christian M Shaffer, Nagesh Chopra, Tao Yang, Bjorn C Knollmann, Dan M Roden, Katherine T Murray
BACKGROUND: The widely used macrolide antibiotic azithromycin increases risk of cardiovascular and sudden cardiac death, although the underlying mechanisms are unclear. Case reports, including the one we document here, demonstrate that azithromycin can cause rapid, polymorphic ventricular tachycardia in the absence of QT prolongation, indicating a novel proarrhythmic syndrome. We investigated the electrophysiological effects of azithromycin in vivo and in vitro using mice, cardiomyocytes, and human ion channels heterologously expressed in human embryonic kidney (HEK 293) and Chinese hamster ovary (CHO) cells...
April 2017: Circulation. Arrhythmia and Electrophysiology
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