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https://www.readbyqxmd.com/read/28646721/biochemical-immunological-hybrid-biosensor-based-on-two-dimensional-chromatography-for-on-site-sepsis-diagnosis
#1
Seung-Wan Kim, Il-Hoon Cho, Guei-Sam Lim, Gi-Na Park, Se-Hwan Paek
A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the rapid two-dimensional (2-D)-chromatography test. To minimize the mutual interference in the hybrid assays, a pre-determined membrane site was etched in a pattern and mounted with a biochemical-reaction pad, thereby allowing a loaded sample to enter and then stay in the pad for a colored-signal production over the course of an immunoassay...
June 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28640772/the-effect-of-a-carbohydrate-mouth-rinse-on-upper-body-muscular-strength-and-endurance
#2
James E Dunkin, Shaun M Phillips
Dunkin, JE and Phillips, SM. The effect of a carbohydrate mouth rinse on upper-body muscular strength and endurance. J Strength Cond Res 31(7): 1948-1953, 2017-Carbohydrate (CHO) mouth rinsing rapidly increases corticomotor output and maximal muscle force production, which could enhance muscular strength and endurance during resistance exercise. However, previous research has found no effect of CHO rinsing on muscular strength or endurance. The current study altered the CHO rinse composition and frequency and the muscular endurance test to further investigate the effects of a CHO mouth rinse on upper-body muscular strength and endurance...
July 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28632394/changes-in-eating-behaviors-of-children-with-obesity-in-response-to-carbohydrate-modified-and-portion-controlled-diets
#3
Shelley Kirk, Jessica G Woo, Bonnie Brehm, Stephen R Daniels, Brian E Saelens
OBJECTIVE: This study's objective was to determine whether two distinct carbohydrate (CHO)-modified diets and a standard portion-controlled (PC) diet differentially impacted children's eating behaviors and whether eating behavior scores predicted lower BMI among children with obesity. METHODS: Children (n = 102) aged 7-12 years with obesity were randomly assigned to a 12-month intervention of a low-carbohydrate (LC), reduced glycemic load (RGL), or standard PC diet...
June 20, 2017: Childhood Obesity
https://www.readbyqxmd.com/read/28605834/absorption-study-of-genistein-using-solid-lipid-microparticles-and-nanoparticles-control-of-oral-bioavailability-by-particle-sizes
#4
Jeong Tae Kim, Sonia Barua, Hyeongmin Kim, Seong-Chul Hong, Seung-Yup Yoo, Hyojin Jeon, Yeongjin Cho, Sangwon Gil, Kyungsoo Oh, Jaehwi Lee
In this study, the effect of particle size of genistein-loaded solid lipid particulate systems on drug dissolution behavior and oral bioavailability was investigated. Genistein-loaded solid lipid microparticles and nanoparticles were prepared with glyceryl palmitostearate. Except for the particle size, other properties of genistein-loaded solid lipid microparticles and nanoparticles such as particle composition and drug loading efficiency and amount were similarly controlled to mainly evaluate the effect of different particle sizes of the solid lipid particulate systems on drug dissolution behavior and oral bioavailability...
June 14, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28604592/carbohydrates-from-sources-with-a-higher-glycemic-index-during-adolescence-is-evening-rather-than-morning-intake-relevant-for-risk-markers-of-type-2-diabetes-in-young-adulthood
#5
Tanja Diederichs, Christian Herder, Sarah Roßbach, Michael Roden, Stefan A Wudy, Ute Nöthlings, Ute Alexy, Anette E Buyken
This study investigated whether glycemic index (GI) or glycemic load (GL) of morning or evening intake and morning or evening carbohydrate intake from low- or higher-GI food sources (low-GI-CHO, higher-GI-CHO) during adolescence are relevant for risk markers of type 2 diabetes in young adulthood. Methods: Analyses included DOrtmund Nutritional and Anthropometric Longitudinally Designed (DONALD) study participants who had provided at least two 3-day weighed dietary records (median: 7 records) during adolescence and one blood sample in young adulthood...
June 10, 2017: Nutrients
https://www.readbyqxmd.com/read/28583516/metabolomics-study-for-identification-of-potential-biomarkers-of-long-term-survival-in-kidney-transplantation-recipients
#6
S-K Lee, B K Kim, H-Y Jung, J-H Cho, S-H Park, Y-L Kim, S-Y Kim, Y-R Yoon, B H Chung, S-H Lee, C-D Kim
BACKGROUND: The recent progress and appropriate use of immunosuppressive drugs have considerably improved the short-term survival in kidney transplantation recipients (KTRs). The development of new strategies to improve long-term survival outcome after kidney transplantation is also becoming important. Although current diagnosis of allograft dysfunction relies on serum creatinine concentration and biopsy, they are nonspecific indicators of allograft function. Therefore, noninvasive, sensitive, and specific biomarkers for the prediction of long-term survival are needed...
June 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28582271/preoperative-oral-carbohydrate-load-versus-placebo-in-major-elective-abdominal-surgery-procy-a-randomized-placebo-controlled-multicenter-phase-iii-trial
#7
Luca Gianotti, Roberto Biffi, Marta Sandini, Daniele Marrelli, Andrea Vignali, Riccardo Caccialanza, Jacopo Viganò, Annarita Sabbatini, Giulio Di Mare, Mario Alessiani, Francesco Antomarchi, Maria Grazia Valsecchi, Davide P Bernasconi
OBJECTIVE: To explore whether preoperative oral carbohydrate (CHO) loading could achieve a reduction in the occurrence of postoperative infections. BACKGROUND: Hyperglycemia may increase the risk of infection. Preoperative CHO loading can achieve postoperative glycemic control. METHODS: This was a randomized, controlled, multicenter, open-label trial. Nondiabetic adult patients who were candidates for elective major abdominal operation were randomized (1:1) to a CHO (preoperative oral intake of 800 mL of water containing 100 g of CHO) or placebo group (intake of 800 mL of water)...
June 2, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28566811/the-effect-of-combined-supplementation-of-carbohydrates-and-creatine-on-anaerobic-performance
#8
A S Theodorou, G Paradisis, E Smpokos, A Chatzinikolaou, I Fatouros, Rfgj King, C B Cooke
The purpose of the study was to examine the effect of creatine (Cr) supplementation on anaerobic performance when ingesting creatine and carbohydrates (CHO) together. Twenty male physical education students comprised the two experimental (CR and CRCHO) and one control (CON) groups of the study. All groups performed three 30 s anaerobic Wingate tests (AWTs) interspersed with 6 minutes of recovery. The CR group (n = 7) ingested 5 g of Cr 5 times per day for 4 days. Subjects in the CRCHO group (n = 6) ingested the same quantity but additionally after each 5 g dose of Cr consumed 500 ml of a commercially available energy drink containing 100 g of simple sugars...
June 2017: Biology of Sport
https://www.readbyqxmd.com/read/28556452/dimensional-alterations-following-vertical-ridge-augmentation-using-collagen-membrane-and-three-types-of-bone-grafting-materials-a-retrospective-observational-study
#9
Yun-Ho Park, Seong-Ho Choi, Kyoo-Sung Cho, Jung-Seok Lee
BACKGROUND: Various biomaterials have been introduced for vertical ridge augmentation to replace autogenous block bone grafting. PURPOSE: This retrospective study radiographically evaluated dimensional alterations of the vertically augmented alveolar ridge using collagen membrane and 3 types of materials: autogenous bone block, allogenous bone block, and particulated bone substitute. MATERIALS AND METHODS: The electronic medical records of 32 patients who received vertical ridge augmentation using 3 types of materials were searched: 9 for autogenous bone block, 12 for allogenous bone block, and 11 for particulated bone substitutes...
May 29, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28552273/intracellular-trehalose-via-transporter-tret1-as-a-method-to-cryoprotect-cho-k1-cells
#10
Tsutomu Uchida, Maho Furukawa, Takahiro Kikawada, Kenji Yamazaki, Kazutoshi Gohara
Trehalose is a promising natural cryoprotectant, but its cryoprotective effect is limited due to difficulties in trans-membrane transport. Thus, expressing the trehalose transporter TRET1 on various mammalian cells may yield more trehalose applications. In this study, we ran comparative cryopreservation experiments between the TRET1-expressing CHO-K1 cells (CHO-TRET1) and the CHO-K1 cells transfected with an empty vector (CHO-vector). The experiments involve freezing under various trehalose concentrations in an extracellular medium...
May 25, 2017: Cryobiology
https://www.readbyqxmd.com/read/28545871/reinforcing-effect-of-glass-fiber-mesh-on-complete-dentures-in-a-test-model-with-a-simulated-oral-mucosa
#11
Sang-Hui Yu, Seunghan Oh, Hye-Won Cho, Ji-Myung Bae
STATEMENT OF PROBLEM: Studies that evaluated the strength of complete dentures reinforced with glass-fiber mesh or metal mesh on a cast with a simulated oral mucosa are lacking. PURPOSE: The purpose of this in vitro study was to compare the mechanical properties of maxillary complete dentures reinforced with glass-fiber mesh with those of metal mesh in a new test model, using a simulated oral mucosa. MATERIAL AND METHODS: Complete dentures reinforced with 2 types of glass-fiber mesh, SES mesh (SES) and glass cloth (GC) and metal mesh (metal) were fabricated...
May 23, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28535549/epidural-dexamethasone-influences-postoperative-analgesia-after-major-abdominal-surgery
#12
Jeong-Min Hong, Kyung-Hoon Kim, Hyeon Jeong Lee, Jae-Young Kwon, Hae-Kyu Kim, Hyae-Jin Kim, Ah-Reum Cho, Wang-Seok Do, Hyo Sung Kim
BACKGROUND: Epidurally administered dexamethasone might reduce postoperative pain. However, the effect of epidural administration of dexamethasone on postoperative epidural analgesia in major abdominal surgery has been doubtful. OBJECTIVES: To investigate the effects and optimal dose of epidural dexamethasone on pain after major abdominal surgery. STUDY DESIGN: A prospective randomized, double-blind study. SETTING: University hospital...
May 2017: Pain Physician
https://www.readbyqxmd.com/read/28504349/polysome-profiling-of-mab-producing-cho-cell-lines-links-translational-control-of-cell-proliferation-and-recombinant-mrna-loading-onto-ribosomes-with-global-and-recombinant-protein-synthesis
#13
Charlotte L Godfrey, Emma J Mead, Olalekan Daramola, Sarah Dunn, Diane Hatton, Ray Field, Gary Pettman, C Mark Smales
mRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to reprogramming of translation and decreased global protein synthesis. Here we apply polysome profiling to determine reprogramming and translational capabilities in host and recombinant monoclonal antibody-producing (mAb) CHO cell lines during batch culture. Recombinant cell lines with the fastest cell specific growth rates were those with the highest global translational efficiency...
May 15, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28500977/endocytosis-and-intracellular-traffic-of-cholesterol-pdmaema-liposome-complexes-in-human-epithelial-like-cells
#14
F Szymanowski, A A Hugo, P Alves, P N Simões, A Gómez-Zavaglia, Pablo F Pérez
Liposomes are generally used as delivery systems, as they are capable of encapsulating a wide variety of molecules (i.e. plasmids, recombinant proteins, therapeutic drugs). However, liposomal drug delivery have to fulfill different requirements, such as the effective internalization by the target cells and avoidance of the degradative activity of the intracellular compartments. The use of polymer lipid complexes (PLCs), by including different polymers in the liposome formulation, could improve internalization and intracellular release of drugs...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28498800/locally-applied-5-fluorouracil-loaded-slow-release-patch-prevents-pancreatic-cancer-growth-in-an-orthotopic-mouse-model
#15
In Kyong Shim, Hye-Jin Yi, Hee-Gyeong Yi, Chan Mi Lee, Yu Na Lee, Yeong-Jin Choi, Seong-Yun Jeong, Eunsung Jun, Robert M Hoffman, Dong-Woo Cho, Song Cheol Kim
To obtain improved efficacy against pancreatic cancer, we investigated the efficacy and safety of a locally-applied 5-fluorouracil (5-FU)-loaded polymeric patch on pancreatic tumors in an orthotopic nude-mouse model. The 5-FU-releasing polymeric patch was produced by 3D printing. After application of the patch, it released the drug slowly for 4 weeks, and suppressed BxPC-3 pancreas cancer growth. Luciferase imaging of BxPC3-Luc cells implanted in the pancreas was performed longitudinally. The drug patch delivered a 30...
June 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28494339/application-of-protein-a-modified-capillary-channeled-polymer-polypropylene-fibers-to-the-quantitation-of-igg-in-complex-matrices
#16
Hung K Trang, R Kenneth Marcus
Polypropylene (PP) capillary-channeled polymer (C-CP) fibers loaded with recombinant Staphyloccocus aureus protein A (rSPA) were used as an affinity chromatography stationary phase for the quantitation of immunoglobulin G (IgG) in complex biological matrices. Optimization of the chromatographic method regarding mobile phase components and load/elution conditions was performed. The six-minute analysis, including a load step with 12mM phosphate at pH 7.4, an elution step with 0.025% phosphoric acid and a re-equilibration step, was employed for quantitation of IgG1 from 0...
April 22, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28492492/the-effect-of-dietary-glycemic-properties-on-markers-of-inflammation-insulin-resistance-and-body-composition-in-postmenopausal-american-women-an-ancillary-study-from-a-multicenter-protein-supplementation-trial
#17
Violeta Stojkovic, Christine A Simpson, Rebecca R Sullivan, Anna Maria Cusano, Jane E Kerstetter, Anne M Kenny, Karl L Insogna, Jessica D Bihuniak
Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance. We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population...
May 11, 2017: Nutrients
https://www.readbyqxmd.com/read/28492302/metal-organic-framework-templated-catalysts-dual-sensitization-of-pdo-zno-composite-on-hollow-sno2-nanotubes-for-selective-acetone-sensors
#18
Won-Tae Koo, Ji-Soo Jang, Seon-Jin Choi, Hee-Jin Cho, Il-Doo Kim
Metal-organic framework (MOF)-derived synergistic catalysts were easily functionalized on hollow SnO2 nanotubes (NTs) via electrospinning and subsequent calcination. Nanoscale Pd NPs (∼2 nm) loaded Zn-based zeolite imidazole framework (Pd@ZIF-8, ∼80 nm) was used as a new catalyst-loading platform for the effective functionalization of a PdO@ZnO complex catalyst onto the thin wall of one-dimensional metal oxide NTs. The well-dispersed nanoscale PdO catalysts (3-4 nm) and multiheterojunctions (PdO/ZnO and ZnO/SnO2) on hollow structures are essential for the development of high-performance gas sensors...
May 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28483700/chemosensitizing-indomethacin-conjugated-chitosan-oligosaccharide-nanoparticles-for-tumor-targeted-drug-delivery
#19
Jae-Young Lee, Ubonvan Termsarasab, Mee Yeon Lee, Dong-Hwan Kim, Song Yi Lee, Jung Sun Kim, Hyun-Jong Cho, Dae-Duk Kim
A chitosan oligosaccharide (CSO)-indomethacin (IDM) conjugate (CI) was synthesized to fabricate chemosensitizing nanoparticles (NPs) for tumor-targeted drug delivery. IDM was conjugated to a CSO backbone via amide bond formation, of which successful synthesis was confirmed by proton-nuclear magnetic resonance analyses. Doxorubicin (DOX)-loaded CI (CI10/DOX; CI:DOX = 10:1 [w/w]) NPs with < 75 nm of mean diameter, polydispersity index of ∼0.2, and positive zeta potential were prepared. The release of DOX from the NPs was enhanced at acidic pH (pH 5...
May 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28470059/enhancement-of-pool-boiling-heat-transfer-using-aligned-silicon-nanowire-arrays
#20
Dong Il Shim, Geehong Choi, Namkyu Lee, Taehwan Kim, Beom Seok Kim, Hyung Hee Cho
Enhancing the critical heat flux (CHF), which is the capacity of heat dissipation, is important to secure high stability in two-phase cooling systems. Coolant supply to a dry hot spot is a major mechanism to prevent surface burn-out for enhancing the CHF. Here, we demonstrate a more ready supply of coolant using aligned silicon nanowires (A-SiNWs), with a high aspect ratio (>10) compared to that of conventional random silicon nanowires (R-SiNWs), which have a disordered arrangement, for additional CHF improvement...
May 12, 2017: ACS Applied Materials & Interfaces
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