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https://www.readbyqxmd.com/read/27913954/the-anti-inflammatory-activities-of-two-major-withanolides-from-physalis-minima-via-acting-on-nf-%C3%AE%C2%BAb-stat3-and-ho-1-in-lps-stimulated-raw264-7-cells
#1
Rui-Jun Li, Cai-Yun Gao, Chao Guo, Miao-Miao Zhou, Jun Luo, Ling-Yi Kong
Physalis minima has been traditionally used as a folk herbal medicine in China for the treatment of many inflammatory diseases. However, little is known about its anti-inflammatory constituents and associated molecular mechanisms. In our study, withaphysalin A (WA) and 2, 3-dihydro-withaphysalin C (WC), two major withanolide-type compounds, were obtained from the anti-inflammatory fraction of P. minima. Both WA and WC significantly inhibited the production of nitrite oxide (NO), prostaglandin E2 (PGE2), and several pro-inflammatory cytokines, such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-activated RAW264...
December 2, 2016: Inflammation
https://www.readbyqxmd.com/read/27912143/coupling-of-helix-e-f-motion-with-the-o-nitrito-and-2-nitrovinyl-coordination-in-myoglobin
#2
Androulla Ioannou, Alexandra Lambrou, Vangelis Daskalakis, Eftychia Pinakoulaki
Myoglobin (Mb) is known to react slowly with nitirite to form the green pigment by NO2(-) cordination to the heme Fe in the O-binding nitrito (O1NO2) mode and to the heme 2-vinyl position. Nitrite is a powerful oxidizing agent and a biological reservoir for NO that has been implicated in a variety of aerobic biological systems. Accordingly, it is important to elucidate the nature and variety of NO2(-) reaction mechanisms with Mb. We have performed principal component analysis (PCA, or essential dynamics) on Molecular Dynamics trajectories of all MbNO2 coordination states to resolve the most important motions in the protein at 298K...
November 25, 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27911255/euzebyella-marina-sp-nov-isolated-from-seawater
#3
Yan-Jiao Zhang, Jia-Rui Zhao, Xi-Ying Zhang, Guo-Zhong Chen, Ming-Yang Zhou, Xu-Hua Mo, Hai-Lun He, Shiyong Chen
A Gram-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase- and catalase- positive bacterium, designated CY01T, was isolated from seawater of the Yellow Sea. Strain CY01T grew at 15-37 C (optimum, 30 C), at pH 5-8 (optimum, 6.5-7.5) and with 0.5-12 % (w/v) NaCl (optimum, 0.5-3.5 %). It could not produce flexirubin-type pigment or reduce nitrate to nitrite. Strain CY01T showed the highest 16S rRNA gene sequence similarity to type strain of Euzebyella saccharophila (97.0 %) and clustered tightly with the Euzebyella species in the phylogenetic trees based on the 16S rRNA gene sequences...
December 1, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27909733/nitrogen-gas-plasma-treatment-of-bacterial-spores-induces-oxidative-stress-that-damages-the-genomic-dna
#4
Akikazu Sakudo, Yoichi Toyokawa, Tetsuji Nakamura, Yoshihito Yagyu, Yuichiro Imanishi
Gas plasma, produced by a short high‑voltage pulse generated from a static induction thyristor power supply [1.5 kilo pulse/sec (kpps)], was demonstrated to inactivate Geobacillus stearothermophilus spores (decimal reduction time at 15 min, 2.48 min). Quantitative polymerase chain reaction and enzyme‑linked immunosorbent assays further indicated that nitrogen gas plasma treatment for 15 min decreased the level of intact genomic DNA and increased the level of 8-hydroxy-2'-deoxyguanosine, a major product of DNA oxidation...
November 29, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27909430/bacterial-active-community-cycling-in-response-to-solar-radiation-and-their-influence-on-nutrient-changes-in-a-high-altitude-wetland
#5
Verónica Molina, Klaudia Hernández, Cristina Dorador, Yoanna Eissler, Martha Hengst, Vilma Pérez, Chris Harrod
Microbial communities inhabiting high-altitude spring ecosystems are subjected to extreme changes in solar irradiance and temperature throughout the diel cycle. Here, using 16S rRNA gene tag pyrosequencing (cDNA) we determined the composition of actively transcribing bacteria from spring waters experimentally exposed through the day (morning, noon, and afternoon) to variable levels of solar radiation and light quality, and evaluated their influence on nutrient recycling. Solar irradiance, temperature, and changes in nutrient dynamics were associated with changes in the active bacterial community structure, predominantly by Cyanobacteria, Verrucomicrobia, Proteobacteria, and 35 other Phyla, including the recently described Candidate Phyla Radiation (e...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27909228/divergent-outcomes-of-fructose-consumption-on-exercise-capacity-of-rats-friend-or-foe
#6
Angela Sun, An Huang, Elizabeth Kertowidjojo, Su Song, Thomas H Hintze, Dong Sun
To test the hypothesis that high fructose (HF) consumption divergently affects exercise capability, as a function of feeding duration, rats were fed a normal (as control) or a normal caloric diet with HF for 3, 6, 10 and 30 days respectively, and run on a treadmill. Results show that running distance and work were significantly increased, associated with greater exercise oxygen consumption in rats fed HF for 3 (HF-3D) and 6 days, but were decreased in rats fed HF for 30 days (HF-30D) compared to their respective control rats...
December 1, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27906429/the-role-of-the-enos-g894t-and-t-786c-gene-polymorphism-in-the-development-of-ascites-in-cirrhosis
#7
O Yildirim, A Yigit, Y Seckin, E Yesilada, G Gulbay, Y F Cagin, Z Aksungur, Y Bilgic, Y Türkoz, S Yologlu
OBJECTIVE: Increased nitric oxide (NO) production in cirrhotic patients causes splanchnic vasodilation, leading to the development of the hyperdynamic circulatory syndrome. One factor that influences plasma NO concentration is eNOS gene polymorphism; consequently, the aim of this study was to investigate whether the eNOS gene G894T and T-786C polymorphisms play any role in the development of ascites in such patients. PATIENTS AND METHODS: Three groups were created: 70 cirrhotic patients with ascites, 69 cirrhotic participants without ascites (stable cirrhosis), and 60 healthy controls...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27905382/-complexes-of-nitric-oxide-with-hemoglobin-and-paramagnetic-metalloenzymes-in-the-brain-and-blood-of-mammals-after-intermittent-hypoxia
#8
Z V Kuropteva, V P Reutov, L M Baider, A L Krushinsky
AIM: To investigate an effect of hypoxia on the formation of nitric oxide (NO) in the brain and blood of rats and an influence of L-NNA, the NO-synthase inhibitor, and sodium nitrite on NO generation in separate and simultaneous introduction of these compounds in the animal body. MATERIAL AND METHODS: Experiments were conducted on 42 rats of Krushinsky-Molodkina (K-M) line without adaptation to hypoxia and short-term adaptation to hypobaric hypoxia. Animals were intraperitoneally injected with sodium nitrite (0...
2016: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/27904528/neuroprotective-effects-of-erythropoietin-against-oxidant-injury-following-brain-irradiation-an-experimental-study
#9
Gamze Ugurluer, Aysegul Cebi, Handan Mert, Nihat Mert, Meltem Serin, Haldun Sukru Erkal
INTRODUCTION: Radiation therapy (RT) is a major treatment modality, and the central nervous system is a dose-limiting organ in clinical RT. This experimental study aims to present the evaluation of the neuroprotective effects of erythropoietin (EPO) against oxidant injury following brain irradiation in rats. MATERIAL AND METHODS: Forty Wistar rats were randomly assigned to four groups (n = 10 each). In group 1 the rats received no EPO and underwent sham RT. The rats in groups 2 and 3 received EPO...
December 1, 2016: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/27902327/microbaculum-marinum-gen-nov-sp-nov-isolated-from-deep-seawater
#10
Yue Su, Shuaibo Han, Ruijun Wang, Xiaoyun Yu, Geyi Fu, Can Chen, Chongya Zhang, Yu Li, Cong Sun, Min Wu
A Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium, designated HSF11T, was isolated from deep seawater of the South China Sea. Growth was observed at 20-40 °C (optimum, 35 °C), pH 5.0-8.5 (optimum, 7.0-7.5) and with 0-7 % NaCl (optimum, 2 %). Bacteriochlorophyll a and poly-β-hydroxybutyrate (PHB) granules were not detected. Nitrate could be reduced to nitrite. The major fatty acids (≥5 %) of strain HSF11T were C19:0 cyclo ω8c, C18:0 and C16:0. The isoprenoid quinone was Q-10. The total lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), two unknown phospholipids (PL1, PL2), one unknown aminolipid (AL1) and three unknown lipids (L1, L3, L4)...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902275/arcticibacterium-luteifluviistationis-gen-nov-sp-nov-isolated-from-arctic-seawater
#11
Dan-Dan Li, Ming Peng, Ning Wang, Xiu-Juan Wang, Xi-Ying Zhang, Xiu-Lan Chen, Hai-Nan Su, Yu-Zhong Zhang, Mei Shi
A Gram-staining-negative, aerobic, non-motile and yellow-pigmented bacterium, designated strain SM1504T, was isolated from Arctic seawater. It hydrolyzed aesculin and gelatin but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1504T constituted a distinct phylogenetic line within the family Cytophagaceae and was closely related to species of the genera Lacihabitans, Emticicia, Fluviimonas and Leadbetterella, with respect to which low sequence similarities between 88...
November 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902199/marinomonas-blandensis-sp-nov-a-novel-marine-gammaproteobacterium
#12
David R Arahal, Teresa Lucena, M Carmen Macián, María A Ruvira, José M González, Itziar Lekumberri, Jarone Pinhassi, María J Pujalte
A novel Gram-staining-negative, chemoorganotrophic, moderately halophilic, strictly aerobic bacterium, strain MED121T, was isolated from a seawater sample collected at the Blanes Bay Microbial Observatory in the north-western Mediterranean Sea. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to Marinomonas dokdonensis, and other Marinomonas spp. (96.3 % and 93.3-95.7 % sequence similarities, respectively), within the family Oceanospirillaceae...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901641/light-triggered-selective-nitration-of-psbo1-in-isolated-arabidopsis-thylakoid-membranes-is-inhibited-by-photosynthetic-electron-transport-inhibitors
#13
Misa Takahashi, Jun Shigeto, Atsushi Sakamoto, Hiromichi Morikawa
PSBO1 is exclusively nitrated when isolated thylakoid membranes are incubated in a buffer bubbled with nitrogen dioxide (NO2) containing NO2 and nitrite. NO2 is the primary intermediate for this selective nitration. Isolated thylakoid membranes were incubated in NO2-bubbled buffer at 25°C in the light or dark. Protein analysis confirmed the selective nitration of PSBO1. Illumination was found to be essential in PSBO1 nitration. A nitration mechanism whereby nitratable tyrosine residues of PSBO1 are, prior to nitration, selectively photo-oxidized by photosynthetic electron transport to tyrosyl radicals to combine with NO2 to form 3-nitrotyrosine was hypothesized...
November 30, 2016: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/27901381/effects-of-fisetin-on-hyperhomocysteinemia-induced-experimental-endothelial-dysfunction-and-vascular-dementia
#14
Boyina Hemanth Kumar, Ravula Arun Reddy, Jerald Mahesh Kumar, B Dinesh Kumar, Prakash V Diwan
This study was designed to investigate the effects of fisetin (FST) on hyperhomocysteinemia (HHcy)-induced experimental endothelial dysfunction (ED) and vascular dementia (VaD) in rats. Wistar rats were randomly divided into 8 groups: control, vehicle control, l-methionine, FST (5, 10, and 25 mg/kg, p.o.), FST-per se (25 mg/kg, p.o.), and donepezil (0.1 mg/kg, p.o.). l-Methionine administration (1.7 g/kg, p.o.) for 32 days induced HHcy. ED and VaD induced by HHcy were determined by vascular reactivity measurements, behavioral analysis using Morris water maze and Y-maze, along with a biochemical and histological evaluation of thoracic aorta and brain tissues...
August 11, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/27900438/start-up-and-bacterial-community-compositions-of-partial-nitrification-in-moving-bed-biofilm-reactor
#15
Tao Liu, Yan-Jun Mao, Yan-Ping Shi, Xie Quan
Partial nitrification (PN) has been considered as one of the promising processes for pretreatment of ammonium-rich wastewater. In this study, a kind of novel carriers with enhanced hydrophilicity and electrophilicity was implemented in a moving bed biofilm reactor (MBBR) to start up PN process. Results indicated that biofilm formation rate was higher on modified carriers. In comparison with the reactor filled with traditional carriers (start-up period of 21 days), it took only 14 days to start up PN successfully with ammonia removal efficiency and nitrite accumulation rate of 90 and 91%, respectively, in the reactor filled with modified carriers...
November 30, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27898937/an-evaluation-of-the-effectiveness-of-freshcase-technology-to-extend-the-storage-life-of-whole-muscle-pork-and-ground-pork-sausage
#16
X Yang, D R Woerner, K R McCullough, J D Hasty, I Geornaras, G C Smith, J N Sofos, K E Belk
The objective of this study was to identify the maximum time of refrigerated storage before aerobic psychrotrophic bacteria grew to a level indicative of spoilage (7 log cfu/g) or other indicators of spoilage were observed for whole-muscle pork and ground pork sausage packaged using FreshCase technology. Pork chops and pork sausage were packaged using conventional vacuum packaging without nitrite in film (Control) or using FreshCase technology and were compared with respect to microbial counts, pH, instrumental color measurements, lipid oxidation level, and sensory properties...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898784/effect-of-metal-ions-on-the-formation-of-trichloronitromethane-during-chlorination-of-catechol-and-nitrite
#17
Guojuan Gan, Rongwu Mei, Lin Qiu, Huachang Hong, Qingjun Wang, Asit Mazumder, Shikai Wu, Xiangliang Pan, Yan Liang
Catechol, nitrite, and dissolved metals are ubiquitous in source drinking water. Catechol and nitrite have been identified as precursors for halonitromethanes (HNMs), but the effect of metal ions on HNM formation during chlorination remains unclear. The main objective of this study was to investigate the effect of metal ions (Fe, Ti, Al) on the formation of trichloronitromethane (TCNM) (the most representative HNM species in disinfected water) on chlorinating catechol and nitrite. Trichloronitromethane was extracted by methyl tert-butyl ether and detected by gas chromatography...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27897440/association-between-aerobic-exercise-training-effects-of-serum-adropin-level-arterial-stiffness-and-adiposity-in-obese-elderly-adults
#18
Shumpei Fujie, Natsuki Hasegawa, Toshiyuki Kurihara, Kiyoshi Sanada, Takafumi Hamaoka, Motoyuki Iemitsu
Serum levels of adropin, which enhances endothelial cell release of nitric oxide (NO), are lower in obese patients. Although habitual aerobic exercise reduces arterial stiffness and adiposity, the relationship between these effects and circulating levels of adropin remains unclear. The purpose of this study was to determine if serum adropin level is associated with the effects of aerobic exercise training on arterial stiffness and adiposity in obese adults. In Experiment 1, we examined whether serum adropin levels are associated with cardiorespiratory fitness, carotid β-stiffness, plasma nitrite/nitrate (NOx) level, and abdominal visceral fat in 27 normal, 20 overweight, and 25 obese adults (age, 41-79 years)...
September 13, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/27896616/seasonal-effects-of-pre-aeration-on-microbial-processes-for-nitrogen-removal-in-constructed-wetlands
#19
Ling Wang, Tian Li
Seasonal effects of pre-aeration on microbial nitrogen performance in constructed wetlands (CWs) involved with anaerobic ammonium oxidation (anammox) process were investigated in this study. Slow natural re-aeration rate was the inhibiting factor for total nitrogen removal in CW without pre-aeration, and partial nitrification was the main way for nitrite generation. Besides partial nitrification, pre-aeration provided nitrite generation in CWs with an alternative way: nitrate reduction. Advantage of pre-aeration of influent was much different under various temperature ranges...
November 29, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27894754/composite-of-cu-metal-nanoparticles-multiwall-carbon-nanotubes-reduced-graphene-oxide-as-a-novel-and-high-performance-platform-of-the-electrochemical-sensor-for-simultaneous-determination-of-nitrite-and-nitrate
#20
Hasan Bagheri, Ali Hajian, Mosayeb Rezaei, Ali Shirzadmehr
In the present research, we aimed to fabricate a novel electrochemical sensor based on Cu metal nanoparticles on the multiwall carbon nanotubes-reduced graphene oxide nanosheets (Cu/MWCNT/RGO) for individual and simultaneous determination of nitrite and nitrate ions. The morphology of the prepared nanocomposite on the surface of glassy carbon electrode (GCE) was characterized using various methods including scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical impedance spectroscopy...
November 21, 2016: Journal of Hazardous Materials
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