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https://www.readbyqxmd.com/read/28822072/cellular-and-molecular-oxidative-stress-related-effects-in-uterine-myometrial-and-trophoblast-decidual-tissues-after-perigestational-alcohol-intake-up-to-early-mouse-organogenesis
#1
Tamara Anahí Coll, Gabriela Chaufan, Leticia Gabriela Pérez-Tito, Martín Ricardo Ventureira, María Del Carmen Ríos de Molina, Elisa Cebral
The placenta plays a major role in embryo-fetal defects and intrauterine growth retardation after maternal alcohol consumption. Our aims were to determine the oxidative status and cellular and molecular oxidative stress effects on uterine myometrium and trophoblast-decidual tissue following perigestational alcohol intake at early organogenesis. CF-1 female mice were administered with 10% alcohol in drinking water for 17 days prior to and up to day 10 of gestation. Control females received ethanol-free water...
August 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28821380/unique-transcriptional-profile-of-native-persisters-in-escherichia-coli
#2
Shinya Matsumoto, Yuto Kawai, Satoshi Miyagawa, Yuka Iwamoto, Shujiro Okuda, Alicia Sánchez-Gorostiaga, Miguel Vicente, Satoshi Tsuneda
Non-dividing persisters, bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, are among the many causes of the refractory nature of bacterial infections. Here we constructed a recombinant Escherichia coli strain that enables to distinguish non-dividing from dividing cell based on Z-ring during cell division. Then, non-dividing cells and dividing cells were successfully separated using a fluorescence activated cell sorter. The sorted non-dividing cells showed significantly higher tolerance toward ofloxacin than dividing cells, which indicates that persisters were concentrated with the methodology...
August 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28820045/effect-of-air-flow-rate-and-c-n-ratio-on-biological-nitrogen-removal-through-canon-process-treating-reject-water
#3
Seyyed Alireza Mousavi, Mohammad Mehralian, Maryam Khashij, Shaliza Ibrahim
CANON process is a promising way for nitrogen removal in wastewaters with low organic carbon content like reject water. This study investigated the effect of important factor for optimization of CANON process through inhibition of nitrite-oxidizing bacteria (NOB). In acclimation period, complete ammonium removal and 43.3% total N removal were obtained at HRT of 12 h, temperature of 30 ± 0.5 °C and DO equal to. The effect of air flow rate (representative of DO), SRT and C/N were evaluated. Air flow rate (AFR) was most important factor for controlling the process, but the effect of SRT was negligible...
August 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28819754/development-of-the-hybrid-cells-in-series-model-to-simulate-ammonia-nutrient-pollutant-transport-along-the-umgeni-river
#4
Kayode O Olowe, Muthukrishnavellaisamy Kumarasamy
Discharge of organic waste results in high nutrient pollution of the water bodies which is a major menace to the environment. A high quantity of nutrients such as ammonia causes a reduction in the dissolved oxygen level and induces algal growth in the water bodies. Water quality models have been the tools to evaluate the rate at which streams can disperse the pollutants they receive. Many water quality models are flawed either because of their inadequacy to completely simulate the advection component of the pollutant transport, or because of the limited application of the models, due to inaccurate estimation of model parameters...
August 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28818018/effect-of-operational-parameters-and-pd-in-catalyst-in-the-reduction-of-nitrate-using-copper-electrode
#5
Thiago Favarini Beltrame, Vanessa Coelho, Luciano Marder, Jane Zoppas Ferreira, Fernanda Albana Marchesini, Andrea Moura Bernardes
Water with high concentration of nitrate may cause damage to health and to the environment. This study investigated how concentration, current density, flow, pH, the use of Pd/In catalyst and operating mode (constant current density and constant cell potential) have an influence in the electrochemical reduction of nitrate and in the formation of gaseous compounds using copper electrode. Experiments were performed in two-compartment electrolytic cells separated by a cationic membrane with nitrate model solutions prepared as a surrogate of concentrated brines from membrane desalination plants...
August 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28817986/formation-of-artifactual-dmpo-oh-spin-adduct-in-acid-solutions-containing-nitrite-ions
#6
Tetsuya Takayanagi, Hirokazu Kimiya, Tatsushi Ohyama
We investigated aqueous solutions containing nitrite ions and DMPO (5,5-dimethyl-1-pyrroline-N-oxide) by ESR (Electron Spin Resonance) in the pH range from 1 to 6. A DMPO -OH signal was observed below pH 3.0 in the presence of nitrite ions, whereas in the absence of nitrite ion, an extremely weak signal was observed below pH 1.5. Addition of methanol, a hydroxyl radical scavenger, to this system did not lead to the appearance of a detectable DMPO-CH2OH signal. The possibility of this DMPO-OH signal being due to a genuine spin trapping process with hydroxyl radical was therefore ruled out...
August 17, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28816057/mitochondrial-complex-i-reversible-s-nitrosation-improves-bioenergetics-and-is-protective-in-parkinson-s-disease
#7
Chiara Milanese, Victor Tapias, Sylvia Gabriels, Silvia Cerri, Giovanna Levandis, Fabio Blandini, Maria Tresini, Sruti Shiva, J Timothy Greenamyre, Mark T Gladwin, Pier G Mastroberardino
AIMS: The present study was designed to explore the neuroprotective potential of inorganic nitrite as a new therapeutic avenue in Parkinson's disease (PD). RESULTS: Administration of inorganic nitrite ameliorates neuropathology in phylogenetically distinct animal models of PD. Beneficial effects are not confined to prophylactic treatment and occur also if nitrite is administered when the pathogenic cascade is already active. Mechanistically, the effect is mediated by both complex I S-nitrosation, which under nitrite administration is favored over formation of other forms of oxidation, and as well as down-stream activation of the antioxidant Nrf2 pathway...
August 17, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28815720/pharmacological-evidence-of-involvement-of-nitric-oxide-pathway-in-anti-pruritic-effects-of-sumatriptan-in-chloroquine-induced-scratching-in-mice
#8
Nazgol-Sadat Haddadi, Sattar Ostadhadi, Saeed Shakiba, Khashayar Afshari, Nastaran Rahimi, Arash Foroutan, Ahmad-Reza Dehpour
Chloroquine (CQ) induces histamine-independent itch in human and mice. We recently reported the role of intradermal nitric oxide (NO)/ cyclic guanosine monophosphate (cGMP) pathway in CQ-evoked scratching in mice. Chloroquine stimulates neuronal nitric oxide synthase (nNOS) activity to over-producing NO in the skin. Sumatriptan, a 5-hydroxytryptamine 1b/1d receptors (5-HTR1b/1d) agonist, is involved in pain and used to treat migraine and cluster headaches. According to previous studies, sumatriptan inhibits NOS activity...
August 16, 2017: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/28815366/abiotic-nitrate-loss-and-nitrogenous-trace-gas-emission-from-chinese-acidic-forest-soils
#9
Yajing Wang, Wenchao Cao, Xinmu Zhang, Jingheng Guo
There are an increasing number of studies, which have shown the potential importance of abiotic denitrification in nitrogen biogeochemistry through pure chemical coupling between nitrate/nitrite reduction and Fe(II) oxidation. However, there is little direct evidence showing the environmental significance of abiotic nitrate (NO3(-)) reduction in acidic soils. We assessed the magnitude and gaseous product stoichiometry of abiotic nitrate reduction in acidic forest soils based on sterilized anoxic soil incubations at different soil pHs and nitrate loadings...
August 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28815274/a-new-metabolomics-based-strategy-for-identification-of-endogenous-markers-of-urine-adulteration-attempts-exemplified-for-potassium-nitrite
#10
Andrea E Steuer, Kim Arnold, Tom D Schneider, Michael Poetzsch, Thomas Kraemer
Urine adulteration to circumvent positive drug testing represents a problem for toxicological laboratories. While creatinine is a suitable marker for dilution, detection of chemicals is often performed by dipstick tests associated with high rates of false positives. Several methods would be necessary to check for all possible adulterants. Untargeted mass spectrometry (MS) methods used in metabolomics should theoretically allow detecting concentration changes of any endogenous urinary metabolite or presence of new biomarkers produced by chemical adulteration...
August 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28814449/haem-iron-and-nitrate-nitrite-account-for-much-of-the-mortality-increase-associated-with-red-meat-consumption
#11
Sabine Rohrmann, Jakob Linseisen
No abstract text is available yet for this article.
August 16, 2017: Evidence-based Medicine
https://www.readbyqxmd.com/read/28811865/oxidative-biochemistry-disbalance-and-changes-on-proteomic-profile-in-salivary-glands-of-rats-induced-by-chronic-exposure-to-methylmercury
#12
Leonardo Oliveira Bittencourt, Bruna Puty, Senda Charone, Walessa Alana Bragança Aragão, Paulo Mecenas Farias-Junior, Marcia Cristina Freitas Silva, Maria Elena Crespo-Lopez, Aline de Lima Leite, Marilia Afonso Rabelo Buzalaf, Rafael Rodrigues Lima
Methylmercury (MeHg) is one of the most toxic mercury species, which can cause many systemic damages, but little is known about its effect in the salivary glands. This study aimed to analyze the mercury levels, oxidative stress, and proteomic profile in parotid, submandibular, and sublingual salivary glands of rats, after chronic MeHg intoxication. Two groups of twenty male Wistar rats (90 days of age) were used on the experiment. MeHg group was intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg/kg/day for 60 days, while the control group received only oil...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28811126/flavonoids-from-psersimmon-diospyros-kaki-l-leaves-inhibit-proliferation-and-induce-apoptosis-in-pc-3%C3%A2-cells-by-activation-of-oxidative-stress-and-mitochondrial-apoptosis
#13
Yan Ding, Kai Ren, Huanhuan Dong, Fei Song, Jing Chen, Youtian Guo, Yanshan Liu, Weijie Tao, Yali Zhang
Persimmon (Diospyros kaki L.) leaves are extensively used in Chinese medicine and are also excellent source of dietary polyphenols. Here we investigated the antiproliferative and pro-apoptotic activity of the total flavonoids extracted from persimmon leaves (FPL) in PC-3 cells. After treating cells with different concentration of FPL, Quercetin or Rutin for 24 h, MTT and flow cytometry were used to measure the cytotoxicity, apoptotic rate and cell cycle arrest. Compared with Quercetin and Rutin, FPL showed higher cytotoxicity at 12...
August 12, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28810618/sivelestat-sodium-hydrate-improves-post-traumatic-knee-osteoarthritis-through-nuclear-factor-%C3%AE%C2%BAb-in-a-rat-model
#14
Xiaofeng Yu, Lijun Zhao, Zhiping Yu, Changzheng Yu, Jianfei Bi, Binglong Sun, Haibo Cong
As a specific inhibitor of neutrophil elastase, sivelestat sodium hydrate has primarily been used in the treatment of acute lung injury caused by various factors since its approval in 2002. Sivelestat sodium hydrate also improves post-traumatic knee osteoarthritis (KOA), although its underlying mechanisms of action have yet to be elucidated. The aim of the current study was to determine if sivelestat sodium hydrate improves post-traumatic KOA through nuclear factor (NF)-κB in a rat model. Treatment with sivelestat sodium hydrate significantly inhibited the induction of structural changes and significantly increased the vertical episode count and ipsilateral static weight bearing of the joint in KOA rats (all P<0...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810519/the-beneficial-role-of-naringin-a-citrus-bioflavonoid-against-oxidative-stress-induced-neurobehavioral-disorders-and-cognitive-dysfunction-in-rodents-a-systematic-review-and-meta-analysis
#15
REVIEW
Gollapalle Lakshminarayanashastry Viswanatha, H Shylaja, Yogananda Moolemath
OBJECTIVES: Naringin is a bioflavonoid, very abundantly found in citrus species. In literature, naringin has been scientifically well documented for its beneficial effects in various neurological disorders. In this systematic review and meta-analysis, we have made an attempt to correlate the protective role of naringin against oxidative stress-induced neurological disorders in rodents. METHODS: The systematic search was performed using electronic databases; the search was mainly focused on the role of naringin in oxidative stress-induced neuropathological conditions in rodents...
August 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28810505/denitrification-of-the-anaerobic-membrane-bioreactor-anmbr-effluent-with-alternative-electron-donors-in-domestic-wastewater-treatment
#16
L Pelaz, A Gómez, G Garralón, A Letona, M Fdz-Polanco
A fixed film bioreactor for the denitrification of the effluent from an anaerobic membrane bioreactor (AnMBR) treating domestic wastewater was designed, built and investigated. After anaerobic treatment, the wastewater usually has a low C/N ratio (∼1.3), and a remaining chemical oxygen demand of around 117mg O2/L, which is not enough to make conventional heterotrophic denitrification possible. That effluent also holds methane and sulfide dissolved and oversaturated after leaving the AnMBR. This paper demonstrates the feasibility of using these reduced compounds as electron donors in order to remove 80mg NOx(-)-N/L at 18°C and 2h of hydraulic retention time...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28809548/reaction-of-dimethyl-trisulfide-with-hemoglobin
#17
Xinmei Dong, Lorand Kiss, Ilona Petrikovics, David E Thompson
Dimethyl trisulfide (DMTS) is a promising antidotal candidate for cyanide intoxication. DMTS acts as a sulfur donor in the conversion of cyanide to the less-toxic thiocyanate. The alternate reaction pathways of DMTS in the blood are not well understood. We report changes in the hemoglobin absorption spectrum upon reaction with DMTS. These changes closely match those induced by the known methemoglobin former, sodium nitrite. The kinetics of methemoglobin formation with DMTS are slower than with sodium nitrite...
August 15, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28807084/linseed-plus-nitrate-in-the-diet-for-fattening-bulls-effects-on-methane-emission-animal-health-and-residues-in-offal
#18
M Doreau, M Arbre, M Popova, Y Rochette, C Martin
The combination of linseed and nitrate is known to decrease enteric methane emission in dairy cows but few studies have been carried out in fattening cattle for animal liveweight gain, enteric methane emission, animal health and presence of residues in beef products. To address this gap, 16 young bulls received a control (C) diet between weaning at 9 months and 14 months, then were split into two groups of eight balanced on feed intake, BW gain and methane emission to receive either the C diet or a diet moderately supplemented with extruded linseed and calcium nitrate (LN) for 2 months before being slaughtered...
August 15, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28806669/griess-reaction-based-paper-strip-for-colorimetric-fluorescent-sers-triple-sensing-of-nitrite
#19
Dan Li, Yadan Ma, Huazhen Duan, Wei Deng, Dawei Li
This study demonstrates a novel strategy for colorimetric/fluorescent/surface enhanced Raman scattering (SERS) triple-mode sensing of nitrite based on Griess reaction modulated gold nanorods (GNRs)-Azo-gold nanoparticles (GNPs) (GNRs-Azo-GNPs) assembly. The p-aminothiophenol (PATP)-capped GNRs (GNRs@PATP) and 1,8-diaminonaphthalene (DAP)-modified GNPs (GNPs@DAP) are first synthesized through Au-S and Au-N bonds, respectively. Upon being excited at 365nm, the dispersion of GNRs-GNPs (GNRs@PATP and GNPs@DAP) emits cyan fluorescence...
August 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28806073/transformation-among-aromatic-iodinated-disinfection-byproducts-in-the-presence-of-monochloramine-from-monoiodophenol-to-triiodophenol-and-diiodonitrophenol
#20
Tingting Gong, Yuxian Tao, Xiangru Zhang, Shaoyang Hu, Jinbao Yin, Qiming Xian, Jian Ma, Bin Xu
Aromatic iodinated disinfection byproducts (DBPs) are a newly identified category of highly toxic DBPs. Among the identified aromatic iodinated DBPs, 2,4,6-triiodophenol and 2,6-diiodo-4-nitro- phenol have shown relatively widespread occurrence and high toxicity. In this study, we found that 4-iodophenol underwent transformation to form 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol in the presence of monochloramine. The transformation pathways were investigated, the decomposition kinetics of 4-iodophenol and the formation of 2,4,6-triiodophenol and 2,6-diiodo-4-nitrophenol were studied, the factors affecting the transformation were examined, the toxicity change during the transformation was evaluated, and the occurrence of the proposed transformation pathways during chloramination of source water was verified...
August 14, 2017: Environmental Science & Technology
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