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https://www.readbyqxmd.com/read/28531997/sustained-release-of-growth-hormone-and-sodium-nitrite-from-biomimetic-collagen-coating-immobilized-on-silicone-tubes-improves-endothelialization
#1
Nasim Salehi-Nik, Zahra Malaie-Balasi, Ghassem Amoabediny, Seyedeh Parnian Banikarimi, Behrouz Zandieh-Doulabi, Jenneke Klein-Nulend
Biocompatibility of biomedical devices can be improved by endothelialization of blood-contacting parts mimicking the vascular endothelium's function. Improved endothelialization might be obtained by using biomimetic coatings that allow local sustained release of biologically active molecules, e.g. anti-thrombotic and growth-inducing agents, from nanoliposomes. We aimed to test whether incorporation of growth-inducing nanoliposomal growth hormone (nGH) and anti-thrombotic nanoliposomal sodium nitrite (nNitrite) into collagen coating of silicone tubes enhances endothelialization by stimulating endothelial cell proliferation and inhibiting platelet adhesion...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531961/challenges-in-photocatalytic-reduction-of-nitrate-as-a-water-treatment-technology
#2
Heather O'Neal Tugaoen, Sergi Garcia-Segura, Kiril Hristovski, Paul Westerhoff
Management of ubiquitous nitrate contamination in drinking water sources is a major engineering challenge due to its negative impacts from eutrophication to immediate risk to human health. Several water treatment technologies exist to manage nitrate pollution in water sources. However, the most widely used technologies are phase separation treatments. In this context, nanoscale photocatalysis emerges as a highly promising transformative technology capable of reducing nitrate to innocuous nitrogen with noticeable selectivity...
May 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28531854/achieve-efficient-nitrogen-removal-from-real-sewage-in-a-plug-flow-integrated-fixed-film-activated-sludge-ifas-reactor-via-partial-nitritation-anammox-pathway
#3
Yandong Yang, Liang Zhang, Jun Cheng, Shujun Zhang, Baikun Li, Yongzhen Peng
This study tested the feasibility of plug-flow integrated fixed-film activated sludge (IFAS) reactor in applying sewage partial nitritation/anammox (PN/A) process. The IFAS reactor was fed with real pre-treated sewage (C/N ratio=1.3) and operated for 200days. High nitrogen removal efficiency of 82% was achieved with nitrogen removal rates of 0.097±0.019kgN/(m(3)·d). Therefore, plug-flow IFAS reactor could be an alternative to applying sewage PN/A process. Besides, it was found that the stability of sewage PN/A process was significantly affected by residual ammonium...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28529198/nitroalkane-oxidase-structure-and-mechanism
#4
REVIEW
Paul F Fitzpatrick
The flavoprotein nitroalkane oxidase catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones, releasing nitrite and transferring electrons to O2 to form H2O2. A combination of solution and structural analyses have provided a detailed understanding of the mechanism of this enzyme.
May 18, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28527693/two-hit-model-of-schizophrenia-induced-by-neonatal-immune-activation-and-peripubertal-stress-in-rats-study-of-sex-differences-and-brain-oxidative-alterations
#5
Aline Santos Monte, Bruna Stefânia Ferreira Mello, Vládia Célia Moreira Borella, Tatiane da Silva Araujo, Francisco Eliclécio Rodrigues da Silva, Francisca Cléa F de Sousa, Antônio Carlos Pinheiro de Oliveira, Clarissa Severino Gama, Mary V Seeman, Silvânia Maria Mendes Vasconcelos, David Freitas De Lucena, Danielle Macêdo
Schizophrenia is considered to be a developmental disorder with distinctive sex differences. Aiming to simulate the vulnerability of the third trimester of human pregnancy to the developmental course of schizophrenia, an animal model was developed, using neonatal poly(I:C) as a first-hit, and peripubertal stress as a second-hit, i.e. a two-hit model. Since, to date, there have been no references to sex differences in the two-hit model, our study sought to determine sex influences on the development of behavior and brain oxidative change in adult rats submitted to neonatal exposure to poly(I:C) on postnatal days 5-7 as well as peripubertal unpredictable stress (PUS)...
May 17, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28527627/a-kinetic-study-on-the-reactivity-of-azanone-hno-toward-its-selected-scavengers-insight-into-its-chemistry-and-detection
#6
Renata Smulik-Izydorczyk, Adrianna Mesjasz, Angelika Gerbich, Jan Adamus, Radosław Michalski, Adam Sikora
Recently, azanone (HNO), which is the protonated one-electron reduction product of (·)NO, has gained considerable attention due to its unique pharmacological effects. Although there has been much progress in understanding HNO biology and chemistry, it remains the most elusive reactive nitrogen species. Herein, we applied the competition kinetics method, based on two parallel HNO reactions with the different scavengers and molecular oxygen (kO2 = (1.8 ± 0.3) × 10(4) M(-1) s(-1)), to determine the rate constants for the reactions of HNO with its selected co-reactants...
May 17, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28527368/effects-of-parsley-extract-powder-as-an-alternative-for-the-direct-addition-of-sodium-nitrite-in-the-production-of-mortadella-type-sausages-impact-on-microbiological-physicochemical-and-sensory-aspects
#7
Greta Riel, Annika Boulaaba, Johanna Popp, Guenter Klein
Increasing concern about chemical additives in processed meat has led to an increased market of uncured and alternatively cured meat products. However, the use of vegetable extracts or the exclusion of curing salt may increase the risk of greater bacterial growth and alteration of several physicochemical parameters. Therefore, in this study mortadella-type sausages, manufactured with 1.07 (V3), 2.14 (V4) and 4.29 (V5) g parsley extract powder/kg sausage meat were produced. These sausage variants were compared to an uncured (V2) and a traditionally nitrite-cured control (V1)...
May 12, 2017: Meat Science
https://www.readbyqxmd.com/read/28526842/revealing-genome-scale-transcriptional-regulatory-landscape-of-ompr-highlights-its-expanded-regulatory-roles-under-osmotic-stress-in-escherichia-coli-k-12-mg1655
#8
Sang Woo Seo, Ye Gao, Donghyuk Kim, Richard Szubin, Jina Yang, Byung-Kwan Cho, Bernhard O Palsson
A transcription factor (TF), OmpR, plays a critical role in transcriptional regulation of the osmotic stress response in bacteria. Here, we reveal a genome-scale OmpR regulon in Escherichia coli K-12 MG1655. Integrative data analysis reveals that a total of 37 genes in 24 transcription units (TUs) belong to OmpR regulon. Among them, 26 genes show more than two-fold changes in expression level in an OmpR knock-out strain. Specifically, we find that: 1) OmpR regulates mostly membrane-located gene products involved in diverse fundamental biological processes, such as narU (encoding nitrate/nitrite transporter), ompX (encoding outer membrane protein X), and nuoN (encoding NADH:ubiquinone oxidoreductase); 2) by investigating co-regulation of entire sets of genes regulated by other stress-response TFs, stresses are surprisingly independently regulated among each other; and, 3) a detailed investigation of the physiological roles of the newly discovered OmpR regulon genes reveals that activation of narU represents a novel strategy to significantly improve osmotic stress tolerance of E...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526821/influence-of-soil-moisture-on-codenitrification-fluxes-from-a-urea-affected-pasture-soil
#9
Timothy J Clough, Gary J Lanigan, Cecile A M de Klein, Md Sainur Samad, Sergio E Morales, David Rex, Lars R Bakken, Charlotte Johns, Leo M Condron, Jim Grant, Karl G Richards
Intensively managed agricultural pastures contribute to N2O and N2 fluxes resulting in detrimental environmental outcomes and poor N use efficiency, respectively. Besides nitrification, nitrifier-denitrification and heterotrophic denitrification, alternative pathways such as codenitrification also contribute to emissions under ruminant urine-affected soil. However, information on codenitrification is sparse. The objectives of this experiment were to assess the effects of soil moisture and soil inorganic-N dynamics on the relative contributions of codenitrification and denitrification (heterotrophic denitrification) to the N2O and N2 fluxes under a simulated ruminant urine event...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526796/sulfide-induced-dissimilatory-nitrate-reduction-to-ammonium-supports-anammox-in-an-open-water-unit-process-wetland
#10
Zackary L Jones, Justin T Jasper, David L Sedlak, Jonathan O Sharp
Open-water unit process wetlands host a benthic diatomaceous and bacterial assemblage capable of nitrate removal from treated municipal wastewater with unexpected contributions from anammox processes. In exploring mechanistic drivers of anammox, 16S rRNA gene sequencing profiles of the biomat revealed significant microbial community shifts along the flow path and with depth. Notably there was an increasing abundance of sulfate reducers (Desulfococcus and other Deltaproteobacteria) and anammox microorganisms (Brocadiaceae) with depth...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526789/a-novel-iron-dependent-enzyme-that-catalyzes-the-initial-step-in-the-biodegradation-of-n-nitroglycine-by-variovorax-sp-strain-js1663
#11
Kristina M Mahan, Hangping Zheng, Tekle T Fida, Ronald J Parry, David E Graham, Jim C Spain
Nitramines are key constituents of most of the explosives in current use and consequently contaminate soil and groundwater at many military facilities around the world. Toxicity from nitramine contamination poses a health risk to plants and animals, thus understanding how nitramines are biodegraded is critical to environmental remediation. The biodegradation of synthetic nitramine compounds such as hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX) has been studied for decades, but little is known about the catabolism of naturally produced nitramine compounds...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28525785/denitrifying-woodchip-bioreactor-and-phosphorus-filter-pairing-to-minimize-pollution-swapping
#12
Laura E Christianson, Christine Lepine, Philip L Sibrell, Chad Penn, Steven T Summerfelt
Pairing denitrifying woodchip bioreactors and phosphorus-sorbing filters provides a unique, engineered approach for dual nutrient removal from waters impaired with both nitrogen (N) and phosphorus (P). This column study aimed to test placement of two P-filter media (acid mine drainage treatment residuals and steel slag) relative to a denitrifying system to maximize N and P removal and minimize pollution swapping under varying flow conditions (i.e., woodchip column hydraulic retention times (HRTs) of 7.2, 18, and 51 h; P-filter HRTs of 7...
May 11, 2017: Water Research
https://www.readbyqxmd.com/read/28523580/chemical-characterization-of-surface-snow-in-istanbul-nw-turkey-and-their-association-with-atmospheric-circulations
#13
Asli Baysal, Hakki Baltaci, Nil Ozbek, Orhan Destanoglu, Gul Sirin Ustabasi, Gulcin Gumus
The understanding of the impurities in natural snow is important in realizing its atmospheric quality, soil characteristics, and the pollution caused to the environment. Knowledge of the occurrence of major ions and trace metals in the snow in the megacity of Istanbul is very limited. This manuscript attempts to understand the origin of major soluble ions (fluoride, acetate, formate, chlorite, chloride, nitrite, chlorate, bromide, nitrate, sulfate, phosphate, and perchlorate) and some trace metals (Fe, Mn, Cd, Co, Ni, Pb, Zn, Cu) in winter surface snow, collected in Istanbul, Turkey...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28522691/mechanism-of-transmembrane-signaling-by-sensor-histidine-kinases
#14
Ivan Gushchin, Igor Melnikov, Vitaly Polovinkin, Andrii Ishchenko, Anastasia Yuzhakova, Pavel Buslaev, Gleb Bourenkov, Sergei Grudinin, Ekaterina Round, Taras Balandin, Valentin Borshchevskiy, Dieter Willbold, Gordon Leonard, Georg Büldt, Alexander Popov, Valentin Gordeliy
One of the major and essential classes of transmembrane (TM) receptors, present in all domains of life, is sensor histidine kinases (HKs), parts of two-component signaling systems (TCS). The structural mechanisms of transmembrane signaling by these sensors are poorly understood. We present here crystal structures of the periplasmic sensor domain, the TM domain and the cytoplasmic HAMP domain of the Escherichia coli nitrate/nitrite sensor HK NarQ in the ligand-bound and mutated ligand-free states. The structures reveal that the ligand binding induces significant rearrangements and piston-like shifts of TM helices...
May 18, 2017: Science
https://www.readbyqxmd.com/read/28521187/brain-antioxidant-effect-of-mirtazapine-and-reversal-of-sedation-by-its-combination-with-alpha-lipoic-acid-in-a-model-of-depression-induced-by-corticosterone
#15
Tatiana de Queiroz Oliveira, Caren Nádia Soares de Sousa, Germana Silva Vasconcelos, Luciene Costa de Sousa, Anneheydi Araújo de Oliveira, Cláudio Felipe Vasconcelos Patrocínio, Ingridy da Silva Medeiros, José Eduardo Ribeiro Honório Júnior, Michael Maes, Danielle Macedo, Silvânia Maria Mendes Vasconcelos
BACKGROUND: Depression is accompanied by activated neuro-oxidative and neuro-nitrosative pathways, while targeting these pathways has clinical efficacy in depression. This study aimed to investigate the effects of mirtazapine (MIRT) alone and combined with alpha-lipoic acid (ALA) against corticosterone (CORT) induced behavioral and oxidative alterations. METHODS: Male mice received vehicle or CORT 20mg/kg during 14 days. From the 15th to 21st days they were divided in groups administered: vehicle, MIRT 3mg/kg or the combinations MIRT+ALA100 or MIRT+ALA200...
May 11, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28521169/heterotrophic-ammonia-and-nitrate-bio-removal-over-nitrite-hanbon-performance-and-microflora
#16
Wei Li, Zhao-Yang Cai, Zi-Jun Duo, Yao-Feng Lu, Ke-Xin Gao, Ghulam Abbas, Meng Zhang, Ping Zheng
A novel Heterotrophic Ammonia and Nitrate Bio-removal Over Nitrite (Hanbon) process, combining Short Nitrate Reduction (SNR) with Anaerobic Ammonia Oxidation (Anammox), was developed in a lab-scale continuous up-flow reactor. The substrate effects were investigated to characterize the performance of Hanbon process, and the corresponding microflora information was also revealed. Our results showed that the optimal substrate ratio of NH4(+)-N:NO3(-)-N:COD for the Hanbon process was 0.65:1:2.2. The volumetric nitrogen removal rate was up to 9...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28521159/distribution-and-genetic-diversity-of-microbial-populations-in-the-pilot-scale-biofilter-for-simultaneous-removal-of-ammonia-iron-and-manganese-from-real-groundwater
#17
Qingfeng Cheng, Lichao Nengzi, Linlin Bao, Yang Huang, Shengyu Liu, Xiuwen Cheng, Bo Li, Jie Zhang
A pilot-scale biofilter treating real groundwater was developed in this study, which showed that ammonia, iron and manganese were mainly removed at 0.4, 0.4 and 0.8 m of the filter bed, respectively, and the corresponding removal efficiencies were 90.82%, 95.48% and 95.90% in steady phase, respectively. The variation of microbial populations in the biofilter during start-up process was also investigated using high-throughput pyrosequencing (HTP). Results indicated that the main functional microbes for ammonia, iron and manganese removal were Nitrosomonas, Crenothrix and Crenothrix, respectively, which was mainly distributed at 0...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28521105/tert-butyl-nitrite-mediated-domino-synthesis-of-isoxazolines-and-isoxazoles-from-terminal-aryl-alkenes-and-alkynes
#18
Prasenjit Sau, Sourav Kumar Santra, Amitava Rakshit, Bhisma K Patel
A sequential construction of C-C, C-O, C=N and C=O bonds from alkenes leading to the direct synthesis of isoxazolines in the presence of tert-butyl nitrite, quinoline and Sc(OTf)3 catalyst in DCE at 80 oC has been accomplished. An unprecedented consecutive three CH functionalisations of two styrenes are involved during this isoxazoline synthesis. In this radical mediated reaction one half of the aryl alkene is converted into intermediate 2-nitro ketone which serve as a 1,3-dipolarophile and undergo cycloaddition with the other half of unreacted aromatic terminal alkene...
May 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28515652/effects-of-pre-converted-nitrite-from-red-beet-and-ascorbic-acid-on-quality-characteristics-in-meat-emulsions
#19
Yun-Sang Choi, Tae-Kyung Kim, Ki-Hong Jeon, Jong-Dae Park, Hyun-Wook Kim, Ko-Eun Hwang, Young-Boong Kim
We investigated the effects of fermented red beet extract and ascorbic acid on color development in meat emulsions. The pH of meat emulsions containing red beet extract decreased with an increase in the amount of extract added. The redness of the treated meat emulsions was higher than that of the control with no added nitrite or fermented red beet extract (p<0.05), though the redness of the meat emulsions treated with fermented red beet extract only was lower than in that treated with both fermented red beet extract and ascorbic acid (p<0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28513781/mitochondria-rich-cells-changes-induced-by-nitrite-exposure-in-tambaqui-colossoma-macropomum-cuvier-1818
#20
Oscar T F DA Costa, Cleverson A Ramos, Wallice P Duncan, Juliana L V Lameiras, Marisa N Fernandes
The gill mitochondria-rich cells of the juvenile Amazonian fish Colossoma macropomum were analyzed using light and scanning and transmission electron microscopy after 96 h exposure to 0.04 and 0.2 mM nitrite. Although the number of mitochondria-rich cells decreased significantly in the lamellar epithelium, no decrease was found in the interlamellar region of the gill filament. Nitrite exposure caused significant reduction on the apical surface area of individual mitochondria-rich cells (p < 0.05), with a resulting reduction of the fractional area of these cells in both the lamellar and filament epithelium...
May 15, 2017: Anais da Academia Brasileira de Ciências
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