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Sandra Mendoza-Elizalde, Nancy K Arteaga-Resendiz, Pedro Valencia-Mayoral, Raúl C Luna, Sarbelio Moreno-Espinosa, Francisco Arenas-Huertero, Gerardo Zúñiga, Norma Velázquez-Guadarrama
The bacterium Helicobacter pylori exhibits great genetic diversity, and the pathogenic roles of its virulence factors have been widely studied. However, the evolutionary dynamics of H. pylori strains during stomach colonization are not well-characterized. Here, we analyzed the microevolutionary dynamics of the toxigenic strain vacAs1m1, the non-toxigenic strain vacAs2m2, and a combination of both strains in an animal model over time. Meriones unguiculatus were inoculated with the following bacteria: group 1-toxigenic strain vacAs1m1/cagA+/cagE+/babA2+; ST181, group 2-non-toxigenic strain vacAs2m2/cagA+/cagE+/babA2+; ST2901, and group 3-both strains...
2016: Frontiers in Microbiology
S Elvira, I González-Fernández, R Alonso, J Sanz, V Bermejo-Bermejo
The Sierra de Guadarrama mountain range, located at 60 km from Madrid City (Spain), includes high valuable ecosystems following an altitude gradient, some of them protected under the Sierra de Guadarrama National Park. The characteristic Mediterranean climatic conditions and the precursors emitted from Madrid favor a high photochemical production of ozone (O3) in the region. However, very little information is available about the patterns and levels of O3 and other air pollutants in the high elevation areas and their potential effects on vegetation...
December 0: Environmental Monitoring and Assessment
N F Santos-Sánchez, R Valadez-Blanco, B Hernández-Carlos, A Torres-Ariño, P C Guadarrama-Mendoza, R Salas-Coronado
Despite microalgae recently receiving enormous attention as a potential source of biodiesel, their use is still not feasible as an alternative to fossil fuels. Recently, interest in microalgae has focused on the production of bioactive compounds such as polyunsaturated fatty acids (PUFA), which provide microalgae a high added value. Several considerations need to be assessed for optimizing PUFA production from microalgae. Firstly, a microalgae species that produces high PUFA concentrations should be selected, such as Nannochloropsis gaditana, Isochrysis galbana, Phaeodactylum tricornutum, and Crypthecodinium cohnii, with marine species gaining more attention than do freshwater species...
October 2016: Applied Microbiology and Biotechnology
Jeff Donahue, Lisa Anne Hendricks, Marcus Rohrbach, Subhashini Venugopalan, Sergio Guadarrama, Kate Saenko, Trevor Darrell
Models based on deep convolutional networks have dominated recent image interpretation tasks; we investigate whether models which are also recurrent are effective for tasks involving sequences, visual and otherwise. We describe a class of recurrent convolutional architectures which is end-to-end trainable and suitable for large-scale visual understanding tasks, and demonstrate the value of these models for activity recognition, image captioning, and video description. In contrast to previous models which assume a fixed visual representation or perform simple temporal averaging for sequential processing, recurrent convolutional models are "doubly deep" in that they learn compositional representations in space and time...
September 1, 2016: IEEE Transactions on Pattern Analysis and Machine Intelligence
Vianney Cortés-González, Juan Carlos Zenteno, Martín Guzmán-Sánchez, Verónica Giordano-Herrera, Dalia Guadarrama-Vallejo, Narlly Ruíz-Quintero, Cristina Villanueva-Mendoza
Tietz syndrome and Waardenburg syndrome type 2A are allelic conditions caused by MITF mutations. Tietz syndrome is inherited in an autosomal dominant pattern and is characterized by congenital deafness and generalized skin, hair, and eye hypopigmentation, while Waardenburg syndrome type 2A typically includes variable degrees of sensorineural hearing loss and patches of de-pigmented skin, hair, and irides. In this paper, we report two unrelated families with MITF mutations. The first family showed an autosomal dominant pattern and variable expressivity...
September 8, 2016: American Journal of Medical Genetics. Part A
Pilar García-Tobilla, Susana R Solórzano, Iván Salido-Guadarrama, Vanessa González-Covarrubias, Gustavo Morales-Montor, Carlos E Díaz-Otañez, Mauricio Rodríguez-Dorantes
Worldwide, prostate cancer (PCa) is the second cause of death from malignant tumors among men. Establishment of aberrant epigenetic modifications, such as histone post-translational modifications (PTMs) and DNA methylation (DNAme) produce alterations of gene expression that are common in PCa. Genes of the SFRP family are tumor suppressor genes that are frequently silenced by DNA hypermethylation in many cancer types. The SFRP family is composed of 5 members (SFRP1-5) that modulate the WNT pathway, which is aberrantly activated in PCa...
November 30, 2016: Gene
D Rosales-Martinez, L Gutierrez-Xicotencatl, O Badillo-Godinez, D Lopez-Guerrero, A Santana-Calderon, R Cortez-Gomez, O Ramirez-Pliego, F Esquivel-Guadarrama
Rotavirus is the most common cause of acute infectious diarrhea in human neonates and infants. However, the studies aimed at dissecting the anti-virus immune response have been mainly performed in adults. Dendritic cells (DCs) play a crucial role in innate and acquired immune responses. Therefore, it is very important to determine the response of neonatal and infant DCs to rotavirus and to compare it to the response of adult DCs. Thus, we determined the response of monocyte-derived DCs from umbilical cord blood (UCB) and adult peripheral blood (PB) to rotavirus in vitro...
October 2016: Microbial Pathogenesis
Carlos Pineda-Vadillo, Françoise Nau, Catherin Guerin-Dubiard, Julien Jardin, Valérie Lechevalier, Marisa Sanz-Buenhombre, Alberto Guadarrama, Tamás Tóth, Éva Csavajda, Hajnalka Hingyi, Sibel Karakaya, Juhani Sibakov, Francesco Capozzi, Alessandra Bordoni, Didier Dupont
The aim of the present study was to understand to what extent the inclusion of anthocyanins into dairy and egg matrices could affect their stability after processing and their release and solubility during digestion. For this purpose, individual and total anthocyanin content of four different enriched matrices, namely custard dessert, milkshake, pancake and omelettete, was determined after their manufacturing and during in vitro digestion. Results showed that anthocyanin recovery after processing largely varied among matrices, mainly due to the treatments applied and the interactions developed with other food components...
January 1, 2017: Food Chemistry
Sibel Karakaya, Sebnem Simsek, Alper Tolga Eker, Carlos Pineda-Vadillo, Didier Dupont, Beatriz Perez, Blanca Viadel, Marisa Sanz-Buenhombre, Alberto Guadarrama Rodriguez, Zsófia Kertész, Adrienn Hegyi, Alessandra Bordoni, Sedef Nehir El
Anthocyanins, water soluble polyphenols, have been associated with several beneficial health effects. The aim of this study was to determine how the baking process and food matrix affect anthocyanin stability and bioaccessibility in bakery products in order to develop functional foods. Three well known regularly consumed bakery products (buns, breadsticks and biscuits) were enriched with anthocyanin (AC) isolated from grape skin alone or in combination with docosahexaenoic acid (AC + DHA) to reveal knowledge on AC as active ingredients in real food systems rather than pure compounds...
August 10, 2016: Food & Function
Avelina Sotres-Vega, Jaime Villalba-Caloca, Shely Azrad-Daniel, Jazmín A García-Montes, J Luis Torre-Jaime, Isabel Guadarrama-Sánchez, Diana Pérez-Covarrubias, J Alfredo Santibañez-Salgado
The objective of this study is to present a high-fidelity bench model of cryopreserved stomachs that can be used while learning surgical skills. Thirty stomachs were harvested from Wistar rats at the end of non-abdominal research studies. The stomachs were washed with cold saline solution and filled with hyaluronic acid solution. The organs were then placed into cryovials and cryopreserved at -30 °C for 60 days. The stomachs were thawed to room temperature on the day of the surgical skills practice and two full-thickness incisions were made...
2016: Journal of Veterinary Medical Education
Leonóra Szirovicza, Pilar López, Renáta Kopena, Mária Benkő, José Martín, Judit J Pénzes
Here, we report the results of a large-scale PCR survey on the prevalence and diversity of adenoviruses (AdVs) in samples collected randomly from free-living reptiles. On the territories of the Guadarrama Mountains National Park in Central Spain and of the Chafarinas Islands in North Africa, cloacal swabs were taken from 318 specimens of eight native species representing five squamate reptilian families. The healthy-looking animals had been captured temporarily for physiological and ethological examinations, after which they were released...
2016: PloS One
Gabriela Ávila-Villarreal, María Eva González-Trujano, Azucena Ibeth Carballo-Villalobos, Berenice Aguilar-Guadarrama, Sara García-Jiménez, Diana Elizabeth Giles-Rivas, Patricia Castillo-España, Rafael Villalobos-Molina, Samuel Estrada-Soto
ETHNOPHARMACOLOGICAL RELEVANCE: Brickellia cavanillesii (Asteraceae) (Cass.) A. Gray is one of the popular plants consumed in Central America and Mexico for the treatment of several diseases such as hypertension, diabetes and anxiety, among others. AIM OF THE STUDY: To determine the anxiolytic-like effect of B. Cavanillesii and the safety of its use through toxicological studies. MATERIAL AND METHODS: Anxiolytic-like effects of soluble-methanol extract of B...
July 2, 2016: Journal of Ethnopharmacology
Catarina C Ferreira, Xavier Santos, Miguel A Carretero
Background. Reptiles are sensitive to habitat disturbance induced by wildfires but species frequently show opposing responses. Functional causes of such variability have been scarcely explored. In the northernmost limit of the Mediterranean bioregion, lizard species of Mediterranean affinity (Psammodromus algirus and Podarcis guadarramae) increase in abundance in burnt areas whereas Atlantic species (Lacerta schreiberi and Podarcis bocagei) decrease. Timon lepidus, the largest Mediterranean lizard in the region, shows mixed responses depending on the locality and fire history...
2016: PeerJ
Alberto Ivan Salido-Guadarrama, Jorge Gustavo Morales-Montor, Claudia Rangel-Escareño, Elizabeth Langley, Oscar Peralta-Zaragoza, Jose Luis Cruz Colin, Mauricio Rodriguez-Dorantes
At present, prostate-specific antigen (PSA) is used as a clinical biomarker for prostate cancer (PCa) diagnosis; however, a large number of patients with benign prostate hyperplasia (BPH) with PSA levels in the 'gray area' (4-10 ng/ml) are currently subjected to unnecessary biopsy due to overdiagnosis. Certain microRNAs (miRs) have been proven to be useful biomarkers, several of which are detectable in bodily fluids. The present study identified and validated a urinary miR‑based signature to enhance the specificity of PCa diagnosis and to reduce the number of patients with benign conditions undergoing biopsy...
June 2016: Molecular Medicine Reports
Virginia Loza, Esther Berrendero, Elvira Perona, Pilar Mateo
The occurrence and environmental factors responsible for the distribution of benthic cyanobacteria in running waters remain largely unexplored in comparison with those of other aquatic ecosystems. In this study, combined data of ecological characteristics, molecular analysis (based on 16S rRNA gene), and direct microscopic inspection of environmental samples were analyzed in parallel with the morphological characterization of the isolated strains to investigate benthic cyanobacterial diversity in the Guadarrama river (Spain)...
April 2013: Journal of Phycology
David S Pescador, Ángela Sierra-Almeida, Pablo J Torres, Adrián Escudero
Assessing freezing community response and whether freezing resistance is related to other functional traits is essential for understanding alpine community assemblages, particularly in Mediterranean environments where plants are exposed to freezing temperatures and summer droughts. Thus, we characterized the leaf freezing resistance of 42 plant species in 38 plots at Sierra de Guadarrama (Spain) by measuring their ice nucleation temperature, freezing point (FP), and low-temperature damage (LT50), as well as determining their freezing resistance mechanisms (i...
2016: Frontiers in Plant Science
Gloria Gutiérrez-Venegas, Adriana Guadarrama-Solís, Carmen Muñoz-Seca, Juan Antonio Arreguín-Cano
In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting...
2015: International Journal of Clinical and Experimental Pathology
Dolores Azucena Salazar-Piña, Adolfo Pedroza-Saavedra, Aurelio Cruz-Valdez, Eduardo Ortiz-Panozo, Minerva Maldonado-Gama, Lilia Chihu-Amparan, Angelica Nallelhy Rodriguez-Ocampo, Emilia Orozco-Fararoni, Fernando Esquivel-Guadarrama, Lourdes Gutierrez-Xicotencatl
Cervical cancer (CC) is the second most frequent neoplasia among women worldwide. Cancer prevention programs around the world have used the Papanicolaou (Pap) smear as the primary diagnostic test to reduce the burden of CC. Nevertheless, such programs have not been effective in developing countries, thus leading to research on alternative tests for CC screening. During the virus life cycle and in the process toward malignancy, different human papillomavirus (HPV) proteins are expressed, and they induce a host humoral immune response that can be used as a potential marker for different stages of the disease...
February 2016: Medicine (Baltimore)
Martina Neuendorf, Raquel Guadarrama-Gonzalez, Birgit Lamik, Colin R MacKenzie
BACKGROUND: New molecular methods of detecting Clostridium difficile infection (CDI) provide the routine lab with a sensitive random access method to produce results that are available in a shorter time than traditional methods. METHODS: In this prospective study a total of 989 stool specimens were tested over a period of 16 months in parallel using two isothermal amplification assays, AmpliVue® (Quidel) and Illumigene® (Meridian) and the results compared to those from toxigenic culture...
2016: BMC Microbiology
C Rodriguez-Morata, J A Ballesteros-Cánovas, D Trappmann, M Beniston, M Stoffel
Flash floods are a common natural hazard in Mediterranean mountain environments and responsible for serious economic and human disasters. The study of flash flood dynamics and their triggers is a key issue; however, the retrieval of historical data is often limited in mountain regions as a result of short time series and the systematic lack of historical data. In this study, we attempt to overcome data deficiency by supplementing existing records with dendrogeomorphic techniques which were employed in seven mountain streams along the northern slopes of the Guadarrama Mountain range...
April 15, 2016: Science of the Total Environment
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