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https://www.readbyqxmd.com/read/29543748/allopregnanolone-alters-the-gene-expression-profile-of-human-glioblastoma-cells
#1
Carmen J Zamora-Sánchez, Aylin Del Moral-Morales, Ana M Hernández-Vega, Valeria Hansberg-Pastor, Ivan Salido-Guadarrama, Mauricio Rodríguez-Dorantes, Ignacio Camacho-Arroyo
Glioblastomas (GBM) are the most frequent and aggressive brain tumors. In these malignancies, progesterone (P4) promotes proliferation, migration, and invasion. The P4 metabolite allopregnanolone (3α-THP) similarly promotes cell proliferation in the U87 human GBM cell line. Here, we evaluated global changes in gene expression of U87 cells treated with 3α-THP, P4, and the 5α-reductase inhibitor, finasteride (F). 3α-THP modified the expression of 137 genes, while F changed 90. Besides, both steroids regulated the expression of 69 genes...
March 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29541818/aquatic-humic-substances-relationship-between-origin-and-complexing-capacity
#2
María de Jesús González-Guadarrama, Ma Aurora Armienta-Hernández, André H Rosa
Aiming to determine the relationship between source and complexing capacity, humic substances obtained from three sites (Sorocaba and Itapanhau Brasilian rivers, and Xochimilco Lake in Mexico) were studied. Copper, manganese, zinc and arsenic complexing capacity were determined for the three substances under various pH conditions. Results showed similar complexing capacity for the three elements depending on the chemistry of each one and on the physico-chemical conditions. Speciation diagrams showed that these conditions affect both, the humic substances, and the transition metals and arsenic...
March 14, 2018: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29529583/domestic-wastewater-treatment-and-power-generation-in-continuous-flow-air-cathode-stacked-microbial-fuel-cell-effect-of-series-and-parallel-configuration
#3
Edson Baltazar Estrada-Arriaga, Jesús Hernández-Romano, Liliana García-Sánchez, Rosa Angélica Guillén Garcés, Erick Obed Bahena-Bahena, Oscar Guadarrama-Pérez, Gabriela Eleonora Moeller Chavez
In this study, a continuous flow stack consisting of 40 individual air-cathode MFC units was used to determine the performance of stacked MFC during domestic wastewater treatment operated with unconnected individual MFC and in series and parallel configuration. The voltages obtained from individual MFC units were of 0.08-1.1 V at open circuit voltage, while in series connection, the maximum power and current density were 2500 mW/m2 and 500 mA/m2 (4.9 V), respectively. In parallel connection, the maximum power and current density was 5...
March 9, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29495578/cucumis-sativus-aqueous-fraction-inhibits-angiotensin-ii-induced-inflammation-and-oxidative-stress-in-vitro
#4
Celeste Trejo-Moreno, Marisol Méndez-Martínez, Alejandro Zamilpa, Enrique Jiménez-Ferrer, Maria Dolores Perez-Garcia, Omar N Medina-Campos, José Pedraza-Chaverri, María Angélica Santana, Fernando R Esquivel-Guadarrama, Aida Castillo, Jacquelynne Cervantes-Torres, Gladis Fragoso, Gabriela Rosas-Salgado
Inflammation and oxidative stress play major roles in endothelial dysfunction, and are key factors in the progression of cardiovascular diseases. The aim of this study was to evaluate in vitro the effect of three subfractions (SFs) from the Cucumis sativus aqueous fraction to reduce inflammatory factors and oxidative stress induced by angiotensin II (Ang II) in human microvascular endothelial cells-1 (HMEC-1) cells. The cells were cultured with different concentrations of Ang II and 0.08 or 10 μg/mL of SF1, SF2, or SF3, or 10 μmol of losartan as a control...
February 28, 2018: Nutrients
https://www.readbyqxmd.com/read/29487435/thermodynamic-sorption-analysis-and-glass-transition-temperature-of-faba-bean-vicia-faba-l-protein
#5
E Alpizar-Reyes, J Castaño, H Carrillo-Navas, J Alvarez-Ramírez, R Gallardo-Rivera, C Pérez-Alonso, A Y Guadarrama-Lezama
Freeze-dried faba bean ( Vicia faba L.) protein adsorption isotherms were determined at 25, 35 and 40 °C and fitted with the Guggenheim-Anderson-de Boer model. The pore radius of protein was in the range of 0.87-6.44 nm, so that they were considered as micropores and mesopores. The minimum integral entropy ranged between 4.33 and 4.44 kg H2 O/100 kg d.s., was regarded as the point of maximum of stability. The glass transition temperature of the protein equilibrated at the different conditions of storage was determined, showing that the protein remained in glassy state for all cases...
March 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29422768/retinal-phenotypic-characterization-of-patients-with-abca4-retinopathydue-to-the-homozygous-p-ala1773val-mutation
#6
Salvador López-Rubio, Oscar F Chacon-Camacho, Rodrigo Matsui, Dalia Guadarrama-Vallejo, Mirena C Astiazarán, Juan C Zenteno
Purpose: To describe the retinal clinical features of a group of Mexican patients with Stargardt disease carrying the uncommon p.Ala1773Val founder mutation in ABCA4. Methods: Ten patients carrying the p.Ala1773Val mutation, nine of them homozygously, were included. Visual function studies included best-corrected visual acuity, electroretinography, Goldmann kinetic visual fields, and full-field electroretinography (ERG). In addition, imaging studies, such as optical coherence tomography (OCT), short-wave autofluorescence imaging, and quantitative analyses of hypofluorescence, were performed in each patient...
2018: Molecular Vision
https://www.readbyqxmd.com/read/29370528/daily-cycle-in-hepatic-lipid-metabolism-in-obese-mice-neotomodon-alstoni-sex-differences
#7
Moisés Pérez-Mendoza, Julieta Berenice Rivera-Zavala, Asael H Rodríguez-Guadarrama, Luis M Montoya-Gomez, Agustín Carmona-Castro, Mauricio Díaz-Muñoz, Manuel Miranda-Anaya
Disruption of circadian rhythms influences the pathogenesis of obesity, particularly with the basic regulation of food intake and metabolism. A link between metabolism and the circadian clock is the peroxisome proliferator-activated receptors (PPARs). The Neotomodon alstoni mouse, known as the "Mexican volcano mouse," may develop obesity if fed a normo-caloric diet. This manuscript documents the changes in part of the hepatic lipid homeostasis in both sexes of lean and obese N. alstoni mice, comparing the daily changes in the BMAL1 clock protein, in regulators of lipid metabolism (PGC-1α, PPARα-γ, SREBP-1c, and CPT-1α) and in free fatty acid (FFA) and hepatic triacylglyceride (TAG) metabolites in light-dark cycles...
January 25, 2018: Chronobiology International
https://www.readbyqxmd.com/read/29369080/coagulation-assays-in-a-case-of-apixaban-overdose
#8
Dennis S Guadarrama, Sandra M DeMarinis, Joseph D Sweeney
: Intentional overdose of apixaban is rare and minimal data exist regarding the usefulness of routinely available laboratory tests to predict drug levels. A 50-year-old man was admitted after ingestion of 200-mg apixaban. Serial blood samples were obtained over a 54-h period for assessment of the fall-off in drug levels using the prothrombin time/international normalized ratio and anti-Xa assays and compared with an apixaban-specific chromogenic assay. The prothrombin time/international normalized ratio and anti-Xa assays correlated with the apixaban level when the drug was in the supratherapeutic range (>130 ng/ml) but not in the typical therapeutic trough to peak levels...
March 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/29361378/-use-of-its-and-issr-markers-in-the-molecular-characterisation-of-pleurotus-djamor-hybrid-strains
#9
Leticia Aguilar Doroteo, Paola Berenice Zárate Segura, Ramón Villanueva Arce, Jorge Yáñez Fernández, María Eugenia Garín Aguilar, Paula Cecilia Guadarrama Mendoza, Gustavo Valencia Del Toro
BACKGROUND: Molecular characterisation of wild type Pleurotus species is important for germplasm conservation and its further use for genetic improvement. No molecular studies have been performed with monokaryons used for producing hybrid strains, either with the reconstituted strains obtained by pairing those monokaryons. The molecular characterisation of parental dikaryons, hybrid, and reconstituted strains as well as monokaryotic strains, is therefore of utmost importance. AIMS: To carry out the molecular identification of Pleurotus djamor strains, i...
January 2018: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/29352912/synthesis-of-a-poly-ester-dendritic-%C3%AE-cyclodextrin-derivative-by-click-chemistry-combining-the-best-of-two-worlds-for-complexation-enhancement
#10
Luis José López-Méndez, Israel González-Méndez, Rodrigo Aguayo-Ortiz, Laura Dominguez, Sofía L Alcaraz-Estrada, Yareli Rojas-Aguirre, Patricia Guadarrama
In spite of the progress in the cyclodextrins chemistry, the synthesis of monodisperse derivatives with a defined degree of substitution is still a challenge. In this work we present a novel dendritic material produced by combining βCD and second generation poly(ester) dendrons. The selective attachment of dendrons in the seven positions of the βCD-primary face was performed through a CuAAC click reaction, which along with a very simple work-up, allowed obtaining the monodisperse material in very high yields...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29306684/physiological-differences-in-preferred-temperatures-and-evaporative-water-loss-rates-in-two-sympatric-lacertid-species
#11
Marco Sannolo, Frederico M Barroso, Miguel A Carretero
Sister species living in sympatry offer the opportunity to study the degree of divergence in their ecological, physiological and life-history traits. It has been hypothesized that closely related species with overlapping distribution should differ in their niche to reduce competition for resources. Furthermore, the investigation of sympatric species may shed light on how they may coexist without outcompeting each other. In the present study, we assess the degree of physiological divergence in two sympatric lacertid lizards, Podarcis bocagei and Podarcis guadarramae lusitanicus...
February 2018: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/29285620/effect-of-the-combined-treatment-of-albumin-with-plasma-synthesised-pyrrole-polymers-on-motor-recovery-after-traumatic-spinal-cord-injury-in-rats
#12
Omar Fabela-Sánchez, Hermelinda Salgado-Ceballos, Luis Medina-Torres, Laura Álvarez-Mejía, Stephany Sánchez-Torres, Rodrigo Mondragón-Lozano, Axayácatl Morales-Guadarrama, Araceli Díaz-Ruiz, María-Guadalupe Olayo, Guillermo J Cruz, Juan Morales, Camilo Ríos, Roberto Olayo
Traumatic spinal cord injury (TSCI) is a health problem for which there is currently no treatment or definitive therapy. Medicine has explored therapeutic options for patients with TSCI with the aim to improve their quality of life. One alternative has been the development of biomaterials that offer neuroprotection or neuroregeneration of damaged nerve tissue. The microinjection of iodine-doped polypyrrole particles synthesised by plasma (PPPy/I) has shown neuroprotective effects that favour motor function recovery in experimental animals with TSCI...
December 28, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29285077/identification-of-micrornas-in-human-circulating-monocytes-of-postmenopausal-osteoporotic-mexican-mestizo-women-a-pilot-study
#13
Rogelio F Jiménez-Ortega, Eric G Ramírez-Salazar, Alma Y Parra-Torres, Said A Muñoz-Montero, Claudia Rangel-Escareňo, Ivan Salido-Guadarrama, Mauricio Rodriguez-Dorantes, Manuel Quiterio, Jorge Salmerón, Rafael Velázquez-Cruz
MicroRNAs (miRNAs or miRs) are a class of short non-coding RNAs that serve an important regulatory role in living organisms. These molecules are associated with multiple biological processes and are potential biomarkers in multiple diseases. The present study aimed to further identify miRNAs that are differentially expressed in circulating monocytes (CMCs) from postmenopausal Mexican-Mestizo women. Microarray analyses of monocytes using Affymetrix miRNA 4.0 and Human Genome U133 Plus 2.0 arrays were performed in 6 normal and 6 osteoporotic women, followed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29275643/gastric-cancer-in-latin-america
#14
Erika Ruíz-García, Jorge Guadarrama-Orozco, Silvia Vidal-Millán, Leonardo S Lino-Silva, César López-Camarillo, Horacio Astudillo-de la Vega
Every year, cancer affects more than one million Latin Americans. The increasing incidence of cancer could be secondary to an aging population, westernization of life style, and urbanization. LA has among the highest incidence rates of gastric cancer, compared to other countries. In this review, different studies on gastric cancer and its relation with risks factors, such as infections, diet and life styles typical of LA, besides the different molecular alterations of that specific population (mainly at a genetic polymorphism level) are analyzed...
February 2018: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/29245581/a-new-genus-and-species-of-haplobainosomatidae-diplopoda-chordeumatida-from-the-mss-of-the-sierra-de-guadarrama-national-park-central-spain
#15
José D Gilgado, Enrique Ledesma, Henrik Enghoff, Jean-Paul Mauriès, Vicente M Ortuño
The chordeumatidan fauna of the Iberian Peninsula is far from being well known, but recent efforts are improving that knowledge. Samplings carried out in the Milieu Souterrain Superficiel (also known as the Mesovoid Shallow Substratum) on several screes of the Sierra de Guadarrama National Park (central Spain) have led to the discovery of a new species of chordeumatidan millipede. This new species belongs to the family Haplobainosomatidae, which is mainly known from the northern Iberian Peninsula, and thus this is the first record of the family in central Spain...
November 14, 2017: Zootaxa
https://www.readbyqxmd.com/read/29241315/natural-antioxidant-extracts-as-food-preservatives
#16
Norma F Santos-Sánchez, Raúl Salas-Coronado, Rogelio Valadez-Blanco, Beatriz Hernández-Carlos, Paula C Guadarrama-Mendoza
The food industry is becoming more specialized and processing methods are continuously being developed to meet consumer needs. Consumers demand products that are safe and preferably free of synthetic additives. These additives are associated with health effects, in most cases without reasonable justification. Consequently, consumers are looking for clearly labelled products that guarantee the absence of synthetic additives. This has led to the need to search for natural additives, which the food industry claims arenatural antioxidant preservatives...
October 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29239176/development-of-betalain-producing-callus-lines-from-colored-quinoa-varieties-chenopodium-quinoa-willd
#17
Paula Henarejos-Escudero, Berenice Guadarrama-Flores, M Alejandra Guerrero-Rubio, Luz Rayda Gómez-Pando, Francisco García-Carmona, Fernando Gandía-Herrero
Betalains are water-soluble plant pigments of hydrophilic nature with promising bioactive potential. Among the scarce edible sources of betalains is the grain crop quinoa (Chenopodium quinoa Willd), with violet, red, and yellow grains being colored by these pigments. In this work, callus cultures have been developed from differently colored plant varieties. Stable callus lines exhibited color and pigment production when maintained on Murashige and Skoog medium supplemented with the plant growth regulators 6-benzylaminopurine (8...
January 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29236758/distinctive-collembola-communities-in-the-mesovoid-shallow-substratum-first-data-for-the-sierra-de-guadarrama-national-park-central-spain-and-a-description-of-two-new-species-of-orchesella-entomobryidae
#18
Enrique Baquero, Enrique Ledesma, José D Gilgado, Vicente M Ortuño, Rafael Jordana
Two new species of the genus Orchesella Templeton, 1836 have been identified following intensive sampling in the Colluvial Milieu Souterrain Superficiel (Mesovoid Shallow Substratum, or MSS) of the Sierra de Guadarrama using Subterranean Sampling Devices (SSD). The data were obtained from the first extraction of the traps between May and October of 2015. During a study of the Collembola taxon, 32 different genera (61 species) were identified. The highest representative genus presence in almost all traps was Orchesella, with two new species...
2017: PloS One
https://www.readbyqxmd.com/read/29228508/cranial-backspatter-pattern-production-utilizing-human-cadavers
#19
Celestina Rossi, Lynne D Herold, Tom Bevel, Leslie McCauley, Stephanie Guadarrama
A backspatter pattern results from blood drops that travel retrograde to an applied external force. Historically, an array of animals and nonhuman objects have been used to create and study backspatter patterns. In this study, backspatter patterns captured on foam core targets that were placed 45.72 cm (18 in) behind the impact site (occipital area of the skull) were produced by cranial gunshots to human cadavers that were reinfused with fresh defibrinated bovine blood. These patterns were compared to the backspatter patterns produced by shooting blood-soaked sponges, a typical simulant used in controlled studies of backspatter pattern production and characteristics...
December 11, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29225769/fabrication-of-low-cost-micropatterned-polydimethyl-siloxane-scaffolds-to-organise-cells-in-a-variety-of-two-dimensioanl-biomimetic-arrangements-for-lab-on-chip-culture-platforms
#20
Lidia Escutia-Guadarrama, Genaro Vázquez-Victorio, David Martínez-Pastor, Brenda Nieto-Rivera, Marcela Sosa-Garrocho, Marina Macías-Silva, Mathieu Hautefeuille
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications...
January 2017: Journal of Tissue Engineering
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