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https://www.readbyqxmd.com/read/27902410/inhibition-of-quorum-sensing-controlled-virulence-factors-of-pseudomonas-aeruginosa-by-murraya-koenigii-essential-oil-a-study-in-a-caenorhabditis-elegans-infectious-model
#1
P Sankar Ganesh, Ravishankar Vittal Rai
The global emergence of antibiotic resistant strains of Pseudomonas aeruginosa poses a major threat in hospital environments as well as in the community. P. aeruginosa is an opportunistic human pathogen and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) mediates cell-to-cell communication in bacteria and has an important role in regulating virulence genes, antibiotic resistance and biofilm formation which are crucial for establishment of the infection...
November 3, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27901504/evaluation-of-medicinal-plants-and-colloidal-silver-efficiency-against-vibrio-parahaemolyticus-infection-in-litopenaeus-vannamei-cultured-at-low-salinity
#2
María Soledad Morales-Covarrubias, Noemí García-Aguilar, María Del Bolan-Mejía, Ana Carmela Puello-Cruz
In shrimp aquaculture, reduction in the use of synthetic antibiotics is a priority due to the high incidence of resistant bacteria (Vibrio) in the white shrimp Litopenaeus vannamei. An increasing number of studies show bactericidal activity of natural treatments in aquaculture. The effectiveness of neem (Azadirachta indica) and oregano (Lippia berlandieri) aqueous extracts and colloidal silver against V. parahaemolyticus were evaluated in low salinity shrimp culture. Results show that aqueous extracts of oregano and neem each present a minimum inhibitory concentration (MIC) of 62...
November 22, 2016: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/27900891/chondrogenesis-of-human-adipose-derived-stem-cells-for-future-microtia-repair-using-co-culture-technique
#3
Bee See Goh, Siti Nurhadis Che Omar, Muhammad Azhan Ubaidah, Lokman Saim, Shamsul Sulaiman, Kien Hui Chua
CONCLUSION: In conclusion, these result showed HADSCs could differentiate into chondrocytes-like cells, dependent on signaling induced by TGF-β3 and chondrocytes. This is a promising result and showed that HADSCs is a potential source for future microtia repair. The technique of co-culture is a positive way forward to assist the microtia tissue. OBJECTIVE: Reconstructive surgery for the repair of microtia still remains the greatest challenge among the surgeons...
November 30, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27900220/use-of-fibrin-glue-as-a-sealant-at-the-anastomotic-line-in-laparoscopic-pyeloplasty-a-randomised-controlled-trial
#4
Ahmed Farouk, Ahmed Tawfick, Mohamed Kotb, Alaa Abdellmaksoud, Ahmed Safaan, Mohamed Yassin, Hassan Shaker
OBJECTIVE: To assess the effectiveness of fibrin glue as a sealant at the anastomotic line of a stentless laparoscopic pyeloplasty (LPP) repair instead of JJ stent insertion. PATIENTS AND METHODS: In all, 46 patients with pelvi-ureteric junction obstruction scheduled for LPP were randomised into two groups each containing 23 patients. Group A underwent stented repair, while group B had a stentless repair together with sealing of the anastomotic line with fibrin glue...
December 2016: Arab Journal of Urology
https://www.readbyqxmd.com/read/27898352/management-of-the-open-abdomen-using-negative-pressure-wound-therapy-with-instillation-in-severe-abdominal-sepsis-a-review-of-48-cases-in-hospital-mexico-costa-rica
#5
Pablo Sibaja, Alfredo Sanchez, Guillermo Villegas, Alvaro Apestegui, Esteban Mora
INTRODUCTION: Despite the numerous advances in recent years, severe abdominal sepsis (with associated organ failure associated with infection) remains a serious, life-threatening condition with a high mortality rate. OA is a viable alternative to the previously used scheduled repeat laparotomy or continuous peritoneal lavage. The use of Negative Pressure Wound Therapy (NPWT) has been described as a successful method of management of the open abdomen. Adding instillation of saline solution to NPWT in a programmed and controlled manner, could offer the clinician an additional tool for the management of complex septic abdomen...
November 17, 2016: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/27895843/severe-anemia-in-malawian-children
#6
Job Cj Calis, Kamija S Phiri, E Brian Faragher, Bernard J Brabin, Imelda Bates, Luis E Cuevas, Rob J de Haan, Ajib I Phiri, Pelani Malange, Mirriam Khoka, Paul Jm Hulshof, Lisette van Lieshout, Marcel Ghm Beld, Yik Y Teo, Kirk A Rockett, Anna Richardson, Dominic P Kwiatkowski, Malcolm E Molyneux, Michaël Boele van Hensbroek
BACKGROUND: Severe anemia is a major cause of sickness and death in African children, yet the causes of anemia in this population have been inadequately studied. METHODS: We conducted a case-control study of 381 preschool children with severe anemia (hemoglobin concentration, <5.0 g per deciliter) and 757 preschool children without severe anemia in urban and rural settings in Malawi. Causal factors previously associated with severe anemia were studied. The data were examined by multivariate analysis and structural equation modeling...
September 2016: Malawi Medical Journal: the Journal of Medical Association of Malawi
https://www.readbyqxmd.com/read/27894908/implication-of-artemisinin-nematocidal-activity-on-experimental-trichinellosis-in-vitro-and-in-vivo-studies
#7
Dina M Abou Rayia, Abeer E Saad, Dalia S Ashour, Radwa M Oreiby
Benzimidazole drugs are used for treatment of trichinellosis, but they have a limited effect against encapsulated larval stages of Trichinella spiralis. Hence, there is a considerable interest in developing new anthelmintic drugs. Our aim is to investigate the possible effect of artemisinin on T. spiralis in in vitro and in vivo studies. T. spiralis worms were isolated from infected mice and transferred to 3 culture media; group I: with no drugs, group II: contained artemisinin and group III: contained mebendazole, then they were subjected to electron microscopic study...
November 25, 2016: Parasitology International
https://www.readbyqxmd.com/read/27894353/provider-and-user-acceptability-of-intermittent-screening-and-treatment-for-the-control-of-malaria-in-pregnancy-in-malawi
#8
Deborah Almond, Mwayi Madanitsa, Victor Mwapasa, Linda Kalilani-Phiri, Jayne Webster, Feiko Ter Kuile, Lucy Paintain
BACKGROUND: Malaria in pregnancy is a major cause of adverse maternal and fetal outcomes. Intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) is one of the control strategies promoted by WHO. In response to mounting resistance to SP, intermittent screening and treatment (ISTp) has been proposed as an alternative. The objective of this study was to explore the acceptability of ISTp for health workers and pregnant women. METHODS: Semi-structured interviews of ten health workers and five focus group discussions of 38 women enrolled in an ongoing trial comparing IPTp-SP and ISTp with dihydroartemisinin-piperaquine (DP) were conducted at two antenatal clinics in rural Malawi...
November 28, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27891639/consensus-report-faecal-microbiota-transfer-clinical-applications-and-procedures
#9
REVIEW
J König, A Siebenhaar, C Högenauer, P Arkkila, M Nieuwdorp, T Norén, C Y Ponsioen, U Rosien, N G Rossen, R Satokari, A Stallmach, W de Vos, J Keller, R J Brummer
BACKGROUND: Faecal microbiota transplantation or transfer (FMT) aims at replacing or reinforcing the gut microbiota of a patient with the microbiota from a healthy donor. Not many controlled or randomised studies have been published evaluating the use of FMT for other diseases than Clostridium difficile infection, making it difficult for clinicians to decide on a suitable indication. AIM: To provide an expert consensus on current clinical indications, applications and methodological aspects of FMT...
November 27, 2016: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27891182/case-control-study-of-diarrheal-disease-etiology-in-individuals-over-5%C3%A2-years-in-southwest-china
#10
Shun-Xian Zhang, Chun-Li Yang, Wen-Peng Gu, Lin Ai, Emmanuel Serrano, Pin Yang, Xia Zhou, Shi-Zhu Li, Shan Lv, Zhi-Sheng Dang, Jun-Hu Chen, Wei Hu, Li-Guang Tian, Jia-Xu Chen, Xiao-Nong Zhou
BACKGROUND: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. METHODS: 271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27890631/evasion-of-host-immune-defenses-by-human-papillomavirus
#11
REVIEW
Joseph A Westrich, Cody J Warren, Dohun Pyeon
A majority of human papillomavirus (HPV) infections are asymptomatic and self-resolving in the absence of medical interventions. Various innate and adaptive immune responses, as well as physical barriers, have been implicated in controlling early HPV infections. However, if HPV overcomes these host immune defenses and establishes persistence in basal keratinocytes, it becomes very difficult for the host to eliminate the infection. The HPV oncoproteins E5, E6, and E7 are important in regulating host immune responses...
November 24, 2016: Virus Research
https://www.readbyqxmd.com/read/27886596/an-end-point-method-based-on-graphene-oxide-for-rnase-h-analysis-and-inhibitors-screening
#12
Chuan Zhao, Jialong Fan, Lan Peng, Lijian Zhao, Chunyi Tong, Wei Wang, Bin Liu
As a highly conserved damage repair protein, RNase H can hydrolysis DNA-RNA heteroduplex endonucleolytically and cleave RNA-DNA junctions as well. In this study, we have developed an accurate and sensitive RNase H assay based on fluorophore-labeled chimeric substrate hydrolysis and the differential affinity of graphene oxide on RNA strand with different length. This end-point measurement method can detect RNase H in a range of 0.01 to 1 units /mL with a detection limit of 5.0×10(-3) units/ mL under optimal conditions...
November 16, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886039/use-of-personal-digital-assistants-to-detect-healthcare-associated-infections-in-a-neonatal-intensive-care-unit-in-egypt
#13
Elham Am El-Feky, Doa'a A Saleh, Jehan El-Kholy, Ahmed Mahmoud Sayed, Yasmeen Mansi, Mohamed Hashem
INTRODUCTION: Personal digital assistants (PDAs) used in electronic laboratory-based surveillance are a promising alternative to conventional surveillance to detect healthcare-associated infections (HAIs). The aim of the study was to monitor, detect, and analyze HAIs using PDAs in a neonatal intensive care unit (NICU). METHODOLOGY: In this descriptive study, 1,053 neonates admitted to the NICU in the obstetrics and gynecology ward at the Cairo University hospital were included and evaluated for HAIs by collecting data using PDAs programmed by Naval Medical Research Unit 3, Cairo, with the definitions for HAIs provided by the National Healthcare Safety Network of the Centers for Disease Control and Prevention...
November 24, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27882119/bactericidal-effect-of-plasma-jet-with-helium-flowing-through-3-hydrogen-peroxide-against-enterococcus-faecalis
#14
Xin-Cai Zhou, Yu-Lan Li, De-Xi Liu, Ying-Guang Cao, Xin-Pei Lu
The aim of the present study was to assess the antimicrobial activity of plasma jet with helium (He) flowing through 3% hydrogen peroxide in root canals infected with Enterococcus faecalis. A total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with an E. faecalis suspension and incubated for 7 days. Next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without He for different time durations) and one control group treated without plasma...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881690/acceptability-of-outpatient-ready-to-use-food-based-protocols-in-hiv-infected-senegalese-children-and-adolescents-within-the-maggsen-cohort-study
#15
Cecile Cames, Marie Varloteaux, Ndeye Ngone Have, Alhadji Bassine Diom, Philippe Msellati, Ngagne Mbaye, Helene Mbodj, Haby Sy Signate, Aminata Diack
OBJECTIVES: To assess the acceptability of ready-to-use food (RUF)-based outpatient protocols in HIV-infected children and adolescents with severe acute malnutrition (SAM) and moderate acute malnutrition (MAM). METHODS: Plumpy Nut and Plumpy Sup were supplied every 2 weeks and prescribed by weight to SAM and MAM children, respectively. Forty-three children, 24 MAM and 19 SAM, were enrolled. Organoleptic appreciation, feeding modalities, and perceptions surrounding RUF were recorded at week 2...
November 22, 2016: Food and Nutrition Bulletin
https://www.readbyqxmd.com/read/27879980/theory-based-interventions-for-contraception
#16
REVIEW
Laureen M Lopez, Thomas W Grey, Mario Chen, Elizabeth E Tolley, Laurie L Stockton
BACKGROUND: The explicit use of theory in research helps expand the knowledge base. Theories and models have been used extensively in HIV-prevention research and in interventions for preventing sexually transmitted infections (STIs). The health behavior field uses many theories or models of change. However, many educational interventions addressing contraception have no explicit theoretical base. OBJECTIVES: To review randomized controlled trials (RCTs) that tested a theoretical approach to inform contraceptive choice and encourage or improve contraceptive use...
November 23, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27879644/efficacy-of-topical-application-leaf-residue-or-soil-drench-of-blastospores-of-isaria-fumosorosea-for-citrus-root-weevil-management-laboratory-and-greenhouse-investigations
#17
Pasco B Avery, Wayne B Hunter, David G Hall, Mark A Jackson, Charles A Powell
The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 10⁷ blastospores mL(-1) on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls...
November 22, 2016: Insects
https://www.readbyqxmd.com/read/27879485/patients-views-on-fecal-microbiota-transplantation-an-acceptable-therapeutic-option-in-inflammatory-bowel-disease
#18
Jonas Zeitz, Marina Bissig, Christiane Barthel, Luc Biedermann, Sylvie Scharl, Daniel Pohl, Pascal Frei, Stephan R Vavricka, Michael Fried, Gerhard Rogler, Michael Scharl
BACKGROUND: Fecal microbiota transplantation (FMT) represents a new therapeutic option that has been studied in two randomized-controlled trials in ulcerative colitis patients. Our study aimed to identify patients' views on the use of this novel therapeutic approach. METHODS: Using an anonymous questionnaire, we obtained data from 574 inflammatory bowel disease (IBD) patients on their knowledge and willingness to undergo FMT. RESULTS: A large proportion of IBD patients (53...
November 22, 2016: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27878735/a-multi-material-coating-containing-chemically-modified-apatites-for-combined-enhanced-bioactivity-and-reduced-infection-via-a-drop-on-demand-micro-dispensing-technique
#19
Poon Nian Lim, Zuyong Wang, Lei Chang, Toshiisa Konishi, Cleo Choong, Bow Ho, Eng San Thian
Prevention of infection and enhanced osseointegration are closely related, and required for a successful orthopaedic implant, which necessitate implant designs to consider both criteria in tandem. A multi-material coating containing 1:1 ratio of silicon-substituted hydroxyapatite and silver-substituted hydroxyapatite as the top functional layer, and hydroxyapatite as the base layer, was produced via the drop-on-demand micro-dispensing technique, as a strategic approach in the fight against infection along with the promotion of bone tissue regeneration...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27877083/epstein-barr-virus-lytic-reactivation-regulation-and-its-pathogenic-role-in-carcinogenesis
#20
REVIEW
Hongde Li, Sufang Liu, Jianmin Hu, Xiangjian Luo, Namei Li, Ann M Bode, Ya Cao
Epstein-Barr virus (EBV) has been associated with several types of human cancers. In the host, EBV can establish two alternative modes of life cycle, known as latent or lytic and the switch from latency to the lytic cycle is known as EBV reactivation. Although EBV in cancer cells is found mostly in latency, a small number of lytically-infected cells promote carcinogenesis through the release of growth factors and oncogenic cytokines. This review focuses on the mechanisms by which EBV reactivation is controlled by cellular and viral factors, and discusses how EBV lytic infection contributes to human malignancies...
2016: International Journal of Biological Sciences
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