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Salmonella kentucky

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https://www.readbyqxmd.com/read/28160117/characterization-of-salmonella-spp-from-wastewater-used-for-food-production-in-morogoro-tanzania
#1
Ofred J Mhongole, Robinson H Mdegela, Lughano J M Kusiluka, Anita Forslund, Anders Dalsgaard
Wastewater use for crop irrigation and aquaculture is commonly practiced by communities situated close to wastewater treatment ponds. The objective of this study was to characterize Salmonella spp. and their antimicrobial susceptibility patterns among isolates from wastewater and Tilapia fish. A total of 123 Salmonella spp. isolates were isolated from 52 water and 21 fish intestinal samples. Genotyping of Salmonella spp. isolates was done by Pulsed-field Gel Electrophoresis (PFGE). Antimicrobial susceptibility testing was done by the minimal inhibitory concentration (MIC) technique...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28137797/a-whole-genome-sequencing-approach-to-study-cefoxitin-resistant-salmonella-enterica-serovar-heidelberg-from-various-sources
#2
Romaine Edirmanasinghe, Rita Finley, E Jane Parmley, Brent P Avery, Carolee Carson, Sadjia Bekal, George Golding, Michael R Mulvey
This study characterized cefoxitin-resistant and susceptible Salmonella enterica serovar Heidelberg from human, abattoir poultry, and retail poultry to assess the molecular relationships of isolates from these sources in Québec in 2012. Isolates were collected as part of the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). All isolates were subjected to antimicrobial susceptibility testing, polymerase chain reaction (PCR) for CMY-2, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing (WGS)...
January 30, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28124965/-bacteremia-caused-by-ciprofloxacin-resistant-salmonella-serotype-kentucky-a-case-report-and-the-review-of-literature
#3
Tuba Müderris, Fatma Yekta Ürkmez, Şeref Alp Küçüker, Muhammet Fethi Sağlam, Gül Ruhsar Yılmaz, Rahmet Güner, Revasiye Güleşen, Ziya Cibali Açıkgöz
Salmonella infections can be seen in four clinical types, namely gastroenteritis, bacteremia/sepsis, enteric fever and carriage. These infections can result in uncomplicated diarrhea in most cases, but can lead to invasive disease requiring antimicrobial therapy and can be life-threatening in elderly or immunocomprimised patients. Broad-spectrum cephalosporins and fluoroquinolones are crucial options in the treatment of the invasive infections. Ciprofloxacin resistance is rarely seen in non-typhoid Salmonella enterica isolates, and only in S...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28105330/genetic-markers-associated-with-resistance-to-beta-lactam-and-quinolone-antimicrobials-in-non-typhoidal-salmonella-isolates-from-humans-and-animals-in-central-ethiopia
#4
Tadesse Eguale, Josephine Birungi, Daniel Asrat, Moses N Njahira, Joyce Njuguna, Wondwossen A Gebreyes, John S Gunn, Appolinaire Djikeng, Ephrem Engidawork
BACKGROUND: Beta-lactam and quinolone antimicrobials are commonly used for treatment of infections caused by non-typhoidal Salmonella (NTS) and other pathogens. Resistance to these classes of antimicrobials has increased significantly in the recent years. However, little is known on the genetic basis of resistance to these drugs in Salmonella isolates from Ethiopia. METHODS: Salmonella isolates with reduced susceptibility to beta-lactams (n = 43) were tested for genes encoding for beta-lactamase enzymes, and those resistant to quinolones (n = 29) for mutations in the quinolone resistance determining region (QRDR) as well as plasmid mediated quinolone resistance (PMQR) genes using PCR and sequencing...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28012630/antimicrobial-resistance-patterns-of-bovine-salmonella-enterica-isolates-submitted-to-the-wisconsin-veterinary-diagnostic-laboratory-2006-2015
#5
J R Valenzuela, A K Sethi, N A Aulik, K P Poulsen
Salmonellosis on the dairy continues to have a significant effect on animal health and productivity and in the United States. Additionally, Salmonella enterica ssp. enterica causes an estimated 1.2 million cases of human illness annually. Contributing to the morbidity and mortality in both human and domestic animal species is emergence of antimicrobial resistance by Salmonella species and increased incidence of multidrug-resistant isolates. This study describes serotype distribution and the antimicrobial resistance patterns for various Salmonella serotypes isolated from bovine samples submitted to the Wisconsin Veterinary Diagnostic Laboratory (WVDL) over the past 10 yr...
February 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27908385/the-mutagenic-assessment-of-an-electronic-cigarette-and-reference-cigarette-smoke-using-the-ames-assay-in-strains-ta98-and-ta100
#6
D Thorne, I Crooks, M Hollings, A Seymour, C Meredith, M Gaca
Salmonella typhimurium strains TA98 and TA100 were used to assess the mutagenic potential of the aerosol from a commercially available, rechargeable, closed system electronic-cigarette. Results obtained were compared to those for the mainstream smoke from a Kentucky reference (3R4F) cigarette. Two different test matrices were assessed. Aerosol generated from the e-cigarette was trapped on a Cambridge filter pad, eluted in DMSO and compared to cigarette smoke total particulate matter (TPM), which was generated in the same manner for mutagenicity assessment in the Salmonella assay...
December 2016: Mutation Research
https://www.readbyqxmd.com/read/27856587/genome-sequences-of-two-salmonella-enterica-serovar-kentucky-isolates-recovered-from-poultry-carcasses-in-the-united-states
#7
Bradd J Haley, Seon Woo Kim, Karen Liljebjelke, Jean Guard, Jo Ann S Van Kessel
We report here the draft genome sequences of two Salmonella enterica serovar Kentucky eBurstGroup 15 isolates collected from poultry carcasses in Georgia (USA).
November 17, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27793824/salmonella-enterica-serovar-kentucky-flagella-are-required-for-broiler-skin-adhesion-and-caco-2-cell-invasion
#8
Sanaz Salehi, Kevin Howe, Mark L Lawrence, John P Brooks, R Hartford Bailey, Attila Karsi
: Nontyphoidal Salmonella strains are the main source of pathogenic bacterial contamination in the poultry industry. Recently, Salmonella enterica serovar Kentucky has been recognized as the most prominent serovar on carcasses in poultry-processing plants. Previous studies showed that flagella are one of the main factors that contribute to bacterial attachment to broiler skin. However, the precise role of flagella and the mechanism of attachment are unknown. There are two different flagellar subunits (fliC and fljB) expressed alternatively in Salmonella enterica serovars using phase variation...
January 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27776287/antimicrobial-effect-of-the-tunisian-nana-variety-punica-granatum-l-extracts-against-salmonella-enterica-serovars-kentucky-and-enteritidis-isolated-from-chicken-meat-and-phenolic-composition-of-its-peel-extract
#9
Ben Ajmia Wafa, Mohamed Makni, Sonda Ammar, Lamia Khannous, Amal Ben Hassana, Mohamed Bouaziz, Nour Eddine Es-Safi, Radhouane Gdoura
Punica granatum L. is widely recognized for its potency against a broad spectrum of bacterial pathogens. The purpose of this study was to explore the inhibitory and the bactericidal activities of Punica granatum against Salmonella strains. The effect of extracts obtained from different parts (peels, seeds, juice and flowers) of pomegranate and using different solvents against Salmonella enterica serovars Kentucky and Enteritidis isolated from chicken meat was thus investigated. Salmonella strains were identified with the standard API-20E system and confirmed by real time PCR...
January 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27768932/phenotyping-and-genetic-characterization-of-salmonella-enterica-isolates-from-turkey-revealing-arise-of-different-features-specific-to-geography
#10
Sinem Acar, Ece Bulut, Bora Durul, Ilhan Uner, Mehmet Kur, M Dilek Avsaroglu, Hüseyin Avni Kirmaci, Yasar Osman Tel, Fadile Y Zeyrek, Yesim Soyer
192 Food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in Sanliurfa city, Turkey in a year were collected to determine the Salmonella enterica subsp. enterica mosaic in Turkey. 161 Salmonella isolates represented 17 serotypes, 20 sequence types (STs) and 44 PFGE patterns (PTs). 3 serotypes, S. Enteritidis, S. Typhimurium and S. Kentucky, were recovered from three different hosts. The highest discriminatory power was obtained by PFGE (SID=0...
January 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27695032/genomic-and-evolutionary-analysis-of-two-salmonella-enterica-serovar-kentucky-sequence-types-isolated-from-bovine-and-poultry-sources-in-north-america
#11
Bradd J Haley, Seon Woo Kim, James Pettengill, Yan Luo, Jeffrey S Karns, Jo Ann S Van Kessel
Salmonella enterica subsp. enterica serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated S. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia...
2016: PloS One
https://www.readbyqxmd.com/read/27665007/population-dynamics-and-antimicrobial-resistance-of-the-most-prevalent-poultry-associated-salmonella-serotypes
#12
Devendra H Shah, Narayan C Paul, Willium C Sischo, Rocio Crespo, Jean Guard
Salmonella spp. is the most predominant bacterial cause of foodborne gastroenteritis in humans. Due to the risk of human infection associated with poultry products and the prevalence of antimicrobial resistance, Salmonella also poses a significant challenge to commercial poultry production. During the last decade (2002 to 2012), the 12 most prevalent poultry-associated Salmonella serotypes (MPPSTs) were frequently and consistently isolated from poultry products in the United States. These MPPSTs and their percent prevalence in poultry products include Kentucky (4%), Enteritidis (2%) Heidelberg (2%), Typhimurium (2%), S...
September 24, 2016: Poultry Science
https://www.readbyqxmd.com/read/27585964/wgs-for-surveillance-of-antimicrobial-resistance-a-pilot-study-to-detect-the-prevalence-and-mechanism-of-resistance-to-azithromycin-in-a-uk-population-of-non-typhoidal-salmonella
#13
Satheesh Nair, Philip Ashton, Michel Doumith, Steve Connell, Anais Painset, Solomon Mwaigwisya, Gemma Langridge, Elizabeth de Pinna, Gauri Godbole, Martin Day
OBJECTIVES: WGS and phenotypic methods were used to determine the prevalence of azithromycin resistance in Salmonella enterica isolates from the UK and to identify the underlying mechanisms of resistance. METHODS: WGS by Illumina HiSeq was carried out on 683 Salmonella spp. isolates. Known genes associated with azithromycin resistance were detected by WGS using a mapping-based approach. Macrolide resistance determinants were identified and the genomic context of these elements was assessed by various bioinformatics tools...
September 1, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27525393/-serotype-distribution-and-antimicrobial-susceptibilities-of-salmonella-strains-recovered-from-environmental-samples-between-2008-2014
#14
Revasiye Güleşen, Belkıs Levent, Mehmet Üvey, Hasan Bayrak, Mesut Akgeyik
Despite the measures taken and control applications worldwide, Salmonella infections continue to threat the public health. Since these infections also cause significant economical loss, the salmonellas continue to be forefront globally. The determination of Salmonella serotypes and their sources is important for epidemiological point of view. In this study, serotype distribution and antimicrobial resistance of environmental isolates of Salmonella spp. recovered from the poultry farms, that were send for confirmation and serotyping between seven years period, 2008-2014, were evaluated...
July 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27497120/prevalence-and-characterization-of-salmonella-enterica-and-salmonella-bacteriophages-recovered-from-beef-cattle-feedlots-in-south-texas
#15
Yicheng Xie, Jeffrey W Savell, Ashley N Arnold, Kerri B Gehring, Jason J Gill, T Matthew Taylor
Asymptomatic Salmonella carriage in beef cattle is a food safety concern, and the beef feedlot environment may function as a reservoir of this pathogen. The goal of this study was to identify and isolate Salmonella and Salmonella bacteriophages from beef cattle feedlot environments in order to better understand the microbial ecology of Salmonella and identify phages that might be useful as anti-Salmonella beef safety interventions. Three feedlots in south Texas were visited, and 27 distinct samples from each source were collected from dropped feces, feed from feed bunks, drinking water from troughs, and soil in cattle pens (n = 108 samples)...
August 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/27473153/identification-of-salmonella-enterica-serovar-kentucky-genes-involved-in-attachment-to-chicken-skin
#16
Sanaz Salehi, Kevin Howe, John Brooks, Mark L Lawrence, R Hartford Bailey, Attila Karsi
BACKGROUND: Regardless of sanitation practices implemented to reduce Salmonella prevalence in poultry processing plants, the problem continues to be an issue. To gain an understanding of the attachment mechanism of Salmonella to broiler skin, a bioluminescent-based mutant screening assay was used. A random mutant library of a field-isolated bioluminescent strain of Salmonella enterica serovar Kentucky was constructed. Mutants' attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm diameter pieces of circular chicken skin...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27334298/prevalence-and-antimicrobial-resistance-of-salmonella-serovars-isolated-from-poultry-in-ghana
#17
L A Andoh, A Dalsgaard, K Obiri-Danso, M J Newman, L Barco, J E Olsen
Poultry are possible sources of non-typhoidal Salmonella serovars which may cause foodborne human disease. We conducted a cross-sectional study to determine the prevalence of Salmonella serovars in egg-laying hens and broilers at the farm level and their susceptibility to antimicrobials commonly used in the poultry industry in Ghana. Sampling of faeces by a sock method (n = 75), dust (n = 75), feed (n = 10) and drinking water (n = 10) was performed at 75 commercial egg-laying and broiler farms in two regions of Ghana and skin neck (n = 30) at a local slaughterhouse from broilers representing different flocks...
June 23, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27313565/comparison-of-microbial-communities-isolated-from-feces-of-asymptomatic-salmonella-shedding-and-non-salmonella-shedding-dairy-cows
#18
Bradd J Haley, James Pettengill, Sasha Gorham, Andrea Ottesen, Jeffrey S Karns, Jo Ann S Van Kessel
In the United States Salmonella enterica subsp. enterica serotypes Kentucky and Cerro are frequently isolated from asymptomatic dairy cows. However, factors that contribute to colonization of the bovine gut by these two serotypes have not been identified. To investigate associations between Salmonella status and bacterial diversity, as well as the diversity of the microbial community in the dairy cow hindgut, the bacterial and archaeal communities of fecal samples from cows on a single dairy farm were determined by high-throughput sequencing of 16S rRNA gene amplicons...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27300440/emergence-and-serovar-profiling-of-non-typhoidal-salmonellae-nts-isolated-from-gastroenteritis-cases-a-study-from-south-india
#19
Mamatha Ballal, Suganthi Martena Devadas, Vignesh Shetty, Sohan Rodney Bangera, Thandavarayan Ramamurthy, Anirban Sarkar
Human infection with non-typhoidal Salmonella (NTS) serovars is often a neglected and undiagnosed infection in the developing world. Invasive NTS is now being established as having a new and emerging pathogenic role. There is not sufficient data on the prevalence of NTS serovars and their antibiotic susceptibility pattern from India. Faecal specimens collected from patients with acute gastroenteritis were processed to isolate Salmonella according to the standard protocol for a period from January 2011-December 2014...
November 2016: Infectious Diseases
https://www.readbyqxmd.com/read/27258142/whole-genome-dna-sequence-analysis-of-salmonella-subspecies-enterica-serotype-tennessee-obtained-from-related-peanut-butter-foodborne-outbreaks
#20
Mark R Wilson, Eric Brown, Chris Keys, Errol Strain, Yan Luo, Tim Muruvanda, Christopher Grim, Junia Jean-Gilles Beaubrun, Karen Jarvis, Laura Ewing, Gopal Gopinath, Darcy Hanes, Marc W Allard, Steven Musser
Establishing an association between possible food sources and clinical isolates requires discriminating the suspected pathogen from an environmental background, and distinguishing it from other closely-related foodborne pathogens. We used whole genome sequencing (WGS) to Salmonella subspecies enterica serotype Tennessee (S. Tennessee) to describe genomic diversity across the serovar as well as among and within outbreak clades of strains associated with contaminated peanut butter. We analyzed 71 isolates of S...
2016: PloS One
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