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https://www.readbyqxmd.com/read/27912750/fermented-barley-and-soybean-bs-mixture-enhances-intestinal-barrier-function-in-dextran-sulfate-sodium-dss-induced-colitis-mouse-model
#1
Jong Kyu Woo, Seungho Choi, Ju-Hee Kang, Dae Eung Kim, Byung-Serk Hurh, Jong-Eun Jeon, Sun Yeou Kim, Seung Hyun Oh
BACKGROUND: Inflammatory bowel disease (IBD) is characterized by chronic or relapsing immune system activation and inflammation within the gastrointestinal tract. The lack of safety and efficacy of standard therapies, the use of food supplements for managing IBD is increasing, and many studies have reported that various food supplements provide many beneficial effects for the IBD. METHODS: This study aimed to evaluate the anti-colitis effects of dietary supplementation with a fermented barley and soybean mixture (BS) on intestinal inflammation using a murine model of IBD...
December 3, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27901023/rhein-and-rhubarb-similarly-protect-the-blood-brain-barrier-after-experimental-traumatic-brain-injury-via-gp91-phox-subunit-of-nadph-oxidase-ros-erk-mmp-9-signaling-pathway
#2
Yang Wang, Xuegong Fan, Tao Tang, Rong Fan, Chunhu Zhang, Zebing Huang, Weijun Peng, Pingping Gan, Xingui Xiong, Wei Huang, Xi Huang
Oxidative stress chiefly contributes to the disruption of the BBB following traumatic brain injury (TBI). The Chinese herbal medicine rhubarb is a promising antioxidant in treating TBI. Here we performed in vivo and in vitro experiments to determine whether rhubarb and its absorbed bioactive compound protected the BBB after TBI by increasing ZO-1 expression through inhibition of gp91(phox) subunit of NADPH oxidase/ROS/ERK/MMP-9 pathway. Rats were subjected to the controlled cortical impact (CCI) model, and primary rat cortical astrocytes were exposed to scratch-wound model...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27893847/a2a-adenosine-receptor-antagonism-reverts-the-blood-brain-barrier-dysfunction-induced-by-sleep-restriction
#3
Gabriela Hurtado-Alvarado, Emilio Domínguez-Salazar, Javier Velázquez-Moctezuma, Beatriz Gómez-González
Chronic sleep restriction induces blood-brain barrier disruption and increases pro-inflammatory mediators in rodents. Those inflammatory mediators may modulate the blood-brain barrier and constitute a link between sleep loss and blood-brain barrier physiology. We propose that adenosine action on its A2A receptor may be modulating the blood-brain barrier dynamics in sleep-restricted rats. We administrated a selective A2A adenosine receptor antagonist (SCH58261) in sleep-restricted rats at the 10th day of sleep restriction and evaluated the blood-brain barrier permeability to dextrans coupled to fluorescein (FITC-dextrans) and Evans blue...
2016: PloS One
https://www.readbyqxmd.com/read/27888130/protective-role-of-phenylalanine-on-the-ros-induced-oxidative-damage-apoptosis-and-tight-junction-damage-via-nrf2-tor-and-nf-%C3%AE%C2%BAb-signalling-molecules-in-the-gill-of-fish
#4
Lin Feng, Wen Li, Yang Liu, Wei-Dan Jiang, Sheng-Yao Kuang, Jun Jiang, Ling Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study explored the possible preventive effects of dietary phenylalanine (Phe) on antioxidant responses, apoptosis and tight junction protein transcription in the gills of young grass carp (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of Phe (3.4-16.8 g kg(-1)) for 8 weeks. The results showed that Phe deficiency induced protein oxidation and lipid peroxidation by decreasing the glutathione content and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) in fish gill (P < 0...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27886077/magnetofection-enhances-lentiviral-mediated-transduction-of-airway-epithelial-cells-through-extracellular-and-cellular-barriers
#5
Stefano Castellani, Clara Orlando, Annalucia Carbone, Sante Di Gioia, Massimo Conese
Gene transfer to airway epithelial cells is hampered by extracellular (mainly mucus) and cellular (tight junctions) barriers. Magnetofection has been used to increase retention time of lentiviral vectors (LV) on the cellular surface. In this study, magnetofection was investigated in airway epithelial cell models mimicking extracellular and cellular barriers. Bronchiolar epithelial cells (H441 line) were evaluated for LV-mediated transduction after polarization onto filters and dexamethasone (dex) treatment, which induced hemicyst formation, with or without magnetofection...
November 23, 2016: Genes
https://www.readbyqxmd.com/read/27884700/claudin-5-7-and-18-suppress-proliferation-mediated-by-inhibition-of-phosphorylation-of-akt-in-human-lung-squamous-cell-carcinoma
#6
Risa Akizuki, Shun Shimobaba, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Abnormal expression of claudin (CLDN) subtypes has been reported in various solid cancers. However, it is unknown which subtype plays a key role in the regulation of proliferation in cancer cells. The expression of CLDN3-5, 7, and 18 in human lung squamous carcinoma tissues was lower than that in normal tissue. Here, we examined which combination of exogenous CLDNs expression inhibits proliferation and the molecular mechanism using human lung squamous RERF-LC-AI cells. Real-time polymerase chain reaction and western blotting showed that CLDN3-5, 7, and 18 are little expressed in RERF-LC-AI cells...
November 21, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27884393/1-25-dihydroxyvitamin-d3-targeting-vegf-pathway-alleviates-house-dust-mite-hdm-induced-airway-epithelial-barrier-dysfunction
#7
Ruhui Zhang, Hangming Dong, Haijin Zhao, Liqin Zhou, Fei Zou, Shaoxi Cai
BACKGROUND: In our previous studies, we have indentified that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) can alleviate toluene diisocyanate-induced airway epithelial barrier disruption and we also found that vascular endothelial growth factor (VEGF) derived from airway epithelials cells could disrupt epithelial barrier. OBJECTIVE: The study aimed to investigate whether 1,25(OH)2D3 can inhibit house dust mite (HDM) induced airway epithelial barrier dysfunction by regulating the VEGF pathway...
November 16, 2016: Cellular Immunology
https://www.readbyqxmd.com/read/27881199/intestinal-permeability-induced-by-lipopolysaccharide-and-measured-by-lactulose-rhamnose-and-mannitol-sugars-in-chickens
#8
S Gilani, G S Howarth, S M Kitessa, C D Tran, R E A Forder, R J Hughes
Increased intestinal permeability (IP) can lead to compromised health. Limited in vivo IP research has been conducted in chickens. The objectives of the current study were to develop a model of increased IP utilizing lipopolysaccharide (LPS Escherichia coli O55:B5) and to evaluate IP changes using the lactulose, mannitol and rhamnose (LMR) sugar permeability test. In addition, fluorescein isothiocyanate dextran (FITC-d), d-lactate, zonula occludens (ZO-1) and diamine oxidase (DAO) permeability tests were employed...
November 24, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27879681/kuwanon-g-preserves-lps-induced-disruption-of-gut-epithelial-barrier-in-vitro
#9
Hengli Guo, Youhua Xu, Wei Huang, Hua Zhou, Zhaoguang Zheng, Yonghua Zhao, Bao He, Tingting Zhu, Shanshan Tang, Quan Zhu
Defects in the gut epithelial barrier have now been recognized to be responsible for diabetic endotoxemia. In everyday life, Mulberry leaf tea is widely used in Asian nations due to its proposed benefits to health and control of diabetes. Evidence indicates the potential role of Kuwanon G (KWG), a component from Morus alba L., on blocking the gut epithelial barrier. In lipopolysaccharides (LPS)-damaged Caco-2 cells, it was found that KWG increased the viability of cells in a concentration-dependent manner. KWG administration significantly elevated the anti-oxidant abilities via increasing ratio of superoxidase dismutase (SOD)/malondialdehyde (MDA) and decreasing reactive oxygen species (ROS) within the cells...
November 22, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27876210/conditioned-medium-from-ls-174t-goblet-cells-treated-with-oxyresveratrol-strengthens-tight-junctions-in-caco-2-cells
#10
Dahyun Hwang, HyunA Jo, Seonwook Hwang, Jeong-Keun Kim, In-Ho Kim, Young-Hee Lim
BACKGROUND: Strengthening of intestinal tight junctions provides an effective barrier from the external environment. Goblet cell-derived trefoil factor 3 (TFF3) increases transepithelial resistance by upregulating the expression of tight junction proteins. Oxyresveratrol (OXY) is a hydroxyl-substituted stilbene found in the roots, leaves, stems, and fruit of many plants and known to have various biological activities. In this study, we investigated the strengthening effect of OXY on intestinal tight junctions through stimulation of TFF production in goblet cells...
November 18, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27875307/salmonella-enteritidis-effector-avra-stabilizes-intestinal-tight-junctions-via-the-jnk-pathway
#11
Zhijie Lin, Yong-Guo Zhang, Yinglin Xia, Xiulong Xu, Xinan Jiao, Jun Sun
Salmonella pathogenesis studies to date have focused on Salmonella Typhimurium, the pathogenesis of a second major serotype, Salmonella Enteritidis, is poorly understood. Salmonella possess effector proteins that display biochemical activities and modulate the function of host. Here, we generated a deletion mutant of effector AvrA, S.E-AvrA-, and a plasmid-mediated complementary strain, S.E-AvrA-/pAvrA+ (S.E-AvrA+), in Salmonella Enteritidis. Using in vitro and in vivo infection models, we showed that AvrA stabilizes epithelial TJ proteins, such as ZO-1, in human intestinal epithelial cells...
November 15, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27875293/attenuation-of-blood-brain-barrier-breakdown-and-hyperpermeability-by-calpain-inhibition
#12
Himakarnika Alluri, Marcene Grimsley, Chinchusha Anasooya Shaji, Kevin Paul Varghese, Shenyuan L Zhang, Chander Peddaboina, Bobby Robinson, Madhava R Beeram, Jason H Huang, Binu Tharakan
Blood-brain barrier (BBB) breakdown and the associated microvascular hyperpermeability followed by brain edema is a hallmark feature of several brain pathologies including traumatic brain injuries (TBI). Recent studies indicate that pro-inflammatory cytokine interleukin-1β (IL-1β) that is upregulated following traumatic injuries also promotes BBB dysfunction and hyperpermeability but the underlying mechanisms are not clearly known. The objective of this study was to determine the role of calpains in mediating BBB dysfunction and hyperpermeability and to test the effect of calpain inhibition on the BBB following traumatic insults to the brain...
November 8, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27868153/carnosine-attenuates-brain-oxidative-stress-and-apoptosis-after-intracerebral-hemorrhage-in-rats
#13
Rong-Xia Xie, Da-Wei Li, Xi-Chang Liu, Ming-Feng Yang, Jie Fang, Bao-Liang Sun, Zong-Yong Zhang, Xiao-Yi Yang
Carnosine, an endogenous dipeptide (β-alanyl-L-histidine), exerts multiple neuroprotective properties, but its role in intracerebral hemorrhage (ICH) remains unclear. This study investigates the effect of Carnosine on brain injury using the rat ICH model, which is established by type IV collagenase caudatum infusion. The results indicate that intraperitoneal administration of Carnosine (1000 mg/kg) significantly attenuates brain edema, blood-brain barrier (BBB) disruption, oxidative stress, microglia activation and neuronal apoptosis of perihematoma at 72 h following ICH in rats models, as convinced by preventing the disruption of tight junction protein ZO-1, occludin and claudin-5, followed by the decrease of ROS, MDA, 3-NT, 8-OHDG level and the increase of GSH-Px and SOD activity, then followed by the decline of Iba-1, ED-1, active caspase-3 and TUNEL positive cells and the decrease of IL-1β, IL-6, TNF-α, active caspase-3 and cytochrome c level...
November 21, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/27867342/the-family-of-crumbs-genes-and-human-disease
#14
REVIEW
Anne M Slavotinek
The family of vertebrate Crumbs proteins, homologous to Drosophila Crumbs (Crb), share large extracellular domains with epidermal growth factor-like repeats and laminin-globular domains, a single transmembrane domain, and a short intracellular C-terminus containing a single membrane proximal 4.1/ezrin/radixin/moesin-binding domain and PSD-95/Discs large/ZO-1-binding motifs. There are 3 Crb genes in humans - Crumbs homolog-1 (CRB1), Crumbs homolog-2 (CRB2), and Crumbs homolog-3 (CRB3). Bilallelic loss-of-function mutations in CRB1 cause visual impairment, with Leber's congenital amaurosis and retinitis pigmentosa, whereas CRB2 mutations are associated with raised maternal serum and amniotic fluid alpha feto-protein levels, ventriculomegaly/hydrocephalus, and renal disease, ranging from focal segmental glomerulosclerosis to congenital Finnish nephrosis...
October 2016: Molecular Syndromology
https://www.readbyqxmd.com/read/27862275/long-noncoding-rna-zfas1-promotes-the-progression-of-colonic-cancer-by-modulating-zeb1-expression
#15
Changyi Fang, Jianbao Zan, Ben Yue, Chenchen Liu, Chenglong He, Dongwang Yan
BACKGROUND AND AIM: Long non-coding RNA ZFAS1 (zinc finger antisense 1) is frequently amplified in hepatocellular carcinoma and promotes metastasis by increasing zinc finger E-box binding homeobox 1 (ZEB1), which can potentiate the progression of epithelial-to-mesenchymal transition (EMT). However, the expression pattern and role of ZFAS1 in colonic cancer remains unknown. The present study aimed to investigate the role of ZFAS1 and its clinical significance in colonic cancer. METHODS: Paired clinical colonic cancer tissue samples and clinicopathologic characteristics of 73 patients were analyzed...
November 11, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27851800/expression-and-function-of-interleukin-1%C3%AE-induced-neutrophil-gelatinase-associated-lipocalin-in-renal-tubular-cells
#16
Tadayoshi Konno, Rei Nakano, Ryo Mamiya, Hisashi Tsuchiya, Taku Kitanaka, Shinichi Namba, Nanako Kitanaka, Ken Okabayashi, Takanori Narita, Hiroshi Sugiya
Acute kidney injury (AKI) is characterized by a sudden loss of renal function. Early recognition of AKI, especially in critically ill patients, is essential for adequate therapy. Currently, neutrophil gelatinase-associated lipocalin (NGAL) is considered to be an effective biomarker of AKI; however, the regulation of its expression and function in renal tubular cells remains unclear. In this study, we investigated the regulation of the expression and function of NGAL in IL-1β-treated Madin-Darby canine kidney (MDCK) cells as a model of renal tubular cells...
2016: PloS One
https://www.readbyqxmd.com/read/27849035/continuous-exposure-to-non-lethal-doses-of-sodium-iodate-induces-retinal-pigment-epithelial-cell-dysfunction
#17
Xiao-Yu Zhang, Tsz Kin Ng, Mårten Erik Brelén, Di Wu, Jian Xiong Wang, Kwok Ping Chan, Jasmine Sum Yee Yung, Di Cao, Yumeng Wang, Shaodan Zhang, Sun On Chan, Chi Pui Pang
Age-related macular degeneration (AMD), characterized by progressive degeneration of retinal pigment epithelium (RPE), is the major cause of irreversible blindness and visual impairment in elderly population. We previously established a RPE degeneration model using an acute high dose sodium iodate to induce oxidative stress. Here we report findings on a prolonged treatment of low doses of sodium iodate on human RPE cells (ARPE-19). RPE cells were treated continuously with low doses (2-10 mM) of sodium iodate for 5 days...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27846299/hyperglycemia-induces-skin-barrier-dysfunctions-with-impairment-of-epidermal-integrity-in-non-wounded-skin-of-type-1-diabetic-mice
#18
Junko Okano, Hideto Kojima, Miwako Katagi, Takahiko Nakagawa, Yuki Nakae, Tomoya Terashima, Takeshi Kurakane, Mamoru Kubota, Hiroshi Maegawa, Jun Udagawa
Diabetes causes skin complications, including xerosis and foot ulcers. Ulcers complicated by infections exacerbate skin conditions, and in severe cases, limb/toe amputations are required to prevent the development of sepsis. Here, we hypothesize that hyperglycemia induces skin barrier dysfunction with alterations of epidermal integrity. The effects of hyperglycemia on the epidermis were examined in streptozotocin-induced diabetic mice with/without insulin therapy. The results showed that dye leakages were prominent, and transepidermal water loss after tape stripping was exacerbated in diabetic mice...
2016: PloS One
https://www.readbyqxmd.com/read/27845198/hyaluronan-35kda-treatment-protects-mice-from-citrobacter-rodentium-infection-and-induces-epithelial-tight-junction-protein-zo-1-in-vivo
#19
Yeojung Kim, Sean P Kessler, Dana R Obery, Craig R Homer, Christine McDonald, Carol A de la Motte
Maintaining a healthy intestinal barrier, the primary physical barrier between intestinal microbiota and the underlying lamina propria, is critical for optimal health. Epithelial integrity is essential for the prevention of the entrance of luminal contents, such as bacteria and their products, through the large intestinal barrier. In this study, we investigated the protective functions of biosynthetic, specific sized, hyaluronan around 35kDa (HA35) on intestinal epithelium in healthy mice, as well as mice infected Citrobacter rodentium, an established model that mimics infection with a serious human pathogen, enteropathogenic E...
November 11, 2016: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/27833554/n-butylphthalide-alleviates-blood-brain-barrier-impairment-in-rats-exposed-to-carbon-monoxide
#20
Mingjun Bi, Mingwei Zhang, Dadong Guo, Weikang Bi, Bin Liu, Yong Zou, Qin Li
Carbon monoxide (CO) poisoning is one of the most important health concerns and may result in neuropathologic changes and neurologic sequelae. However, few studies have addressed the correlation between CO poisoning and blood-brain barrier (BBB) impairment. In this study, we investigated the effects of N-butylphthalide (NBP) on the expressions of zonula occludens-1 (ZO-1), claudin-5 and aquaporin-4 (AQP-4) proteins in a CO poisoning rat model. The results indicated that the brain water content was obviously increased, and the tight junctions between endothelial cells were disrupted, resulting in significant cerebral edema and BBB dysfunction in a rat model of CO poisoning...
2016: Frontiers in Pharmacology
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