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https://www.readbyqxmd.com/read/28815345/mosapride-stabilizes-intestinal-microbiota-to-reduce-bacterial-translocation-and-endotoxemia-in-ccl4-induced-cirrhotic-rats
#1
Hong Xu, Jingfang Xiong, Jianjun Xu, Shuiming Li, Yang Zhou, Dongya Chen, Xinjun Cai, Jian Ping, Min Deng, Jianyong Chen
BACKGROUND: Impaired intestinal motility may lead to the disruption of gut microbiota equilibrium, which in turn facilitates bacterial translocation (BT) and endotoxemia in cirrhosis. We evaluated the influence of mosapride, a prokinetic agent, on BT and DNA fingerprints of gut microbiota in cirrhotic rats. METHODS: A rat model of cirrhosis was set up via subcutaneous injection of carbon tetrachloride (CCl4). The portal pressure, liver and intestinal damage, plasma endotoxin, BT, and intestinal transit rate (ITR) of cirrhotic rats were determined...
August 16, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28812311/nad-replenishment-with-nicotinamide-mononucleotide-protects-blood-brain-barrier-integrity-and-attenuates-delayed-tpa-induced-haemorrhagic-transformation-after-cerebral-ischemia
#2
Chun-Chun Wei, Yuan-Yuan Kong, Xia-Hua, Guo-Qiang Li, Si-Li Zheng, Ming-He Cheng, Pei Wang, Chao-Yu Miao
BACKGROUND AND PURPOSE: Tissue plasminogen activator (tPA) is the only approved pharmacological therapy for acute brain ischemia; however, a major limitation of tPA is the haemorrhagic transformation followed by tPA treatment. Here, we determine whether nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide biosynthesis, would affect tPA-induced haemorrhagic transformation. EXPERIMENTAL APPROACH: Middle cerebral artery occlusion (MCAO) was achieved in CD1 mice by introducing a filament to the left MCA for 5 hours...
August 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28811708/involvement-of-crf2-signaling-in-enterocyte-differentiation
#3
Benjamin Ducarouge, Marjolaine Pelissier-Rota, Rebecca Powell, Alain Buisson, Bruno Bonaz, Muriel Jacquier-Sarlin
AIM: To determine the role of corticotropin releasing factor receptor (CRF2) in epithelial permeability and enterocyte cell differentiation. METHODS: For this purpose, we used rat Sprague Dawley and various colon carcinoma cell lines (SW620, HCT8R, HT-29 and Caco-2 cell lines). Expression of CRF2 protein was analyzed by fluorescent immunolabeling in normal rat colon and then by western blot in dissociated colonic epithelial cells and in the lysates of colon carcinoma cell lines or during the early differentiation of HT-29 cells (ten first days)...
July 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28809572/antigen-specific-gut-inflammation-and-systemic-immune-responses-induced-by-pro-longing-wheat-gluten-sensitization-in-balb-c-murine-model
#4
Vijaykrishnaraj M, Mohan Kumar Bv, Muthukumar Sp, Nawneet K Kurrey, Pichan Prabhasankar
Gluten-related diseases such as wheat allergy, celiac disease, and gluten intolerance are widespread in the globe to genetically pre-disposed individuals. The present study aims to develop wheat-gluten induced BALB/c murine model for addressing wheat-gluten related disorders by sensitizing the wheat gluten through the route of intraperitoneal and oral challenge in prolonged days. During the sensitization, the sera were collected for specific anti-gliadin antibodies response and pro-inflammatory markers quantification...
August 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28805232/the-protective-effect-of-heme-oxygenase-1-against-intestinal-barrier-dysfunction-in-cholestatic-liver-injury-is-associated-with-nf-%C3%AE%C2%BAb-inhibition
#5
Lijing Zhang, Zhenling Zhang, Bojia Liu, Yanling Jin, Yan Tian, Yi Xin, Zhijun Duan
Heme oxygenase-1 (HO-1) is reported to protect against liver injury, but little is known about its effect on the intestinal barrier in cholestatic liver injury. In this study, we investigated the effects of HO-1 and its enzymatic by-product on intestinal barrier dysfunction in bile duct ligation (BDL) rats and explored the possible mechanism. The HO-1 inducer cobalt protoporphyrin (CoPP) and carbon monoxide-releasing molecule-2 (CORM-2) were used; the expression levels of tight junction (TJ) proteins, intestinal inflammation and NF-κB p65 were measured...
August 9, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28804564/incrna-h19-promotes-tongue-squamous-cell-carcinoma-progression-through-%C3%AE-catenin-gsk3%C3%AE-emt-signaling-via-association-with-ezh2
#6
Da-Ming Zhang, Zhao-Yu Lin, Zhao-Hui Yang, You-Yuan Wang, Di Wan, Jiang-Long Zhong, Pei-Lin Zhuang, Zhi-Quan Huang, Bin Zhou, Wei-Liang Chen
H19 is involved in tumor metastasis and associated with tumor progression. Enhancer of zest homolog 2 (EZH2) is overexpressed in multiple cancer types and correlates with tumor proliferation, epithelial-mesenchymal transition, and poor prognosis. However, the interaction between H19 and EZH2 to promote tongue squamous cell carcinoma (TSCC) progression remains largely uncharacterized. Insitu hybridization and quantitative reverse-transcription PCR (qRT-PCR) were performed to measure H19 expression in primary TSCC and adjacent normal tissues and cell lines...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28804538/exendin-4-assisted-adipose-derived-mesenchymal-stem-cell-therapy-protects-renal-function-against-co-existing-acute-kidney-ischemia-reperfusion-injury-and-severe-sepsis-syndrome-in-rat
#7
Pei-Hsun Sung, Hsin-Ju Chiang, Christopher Glenn Wallace, Chih-Chao Yang, Yen-Ta Chen, Kuan-Hung Chen, Chih-Hung Chen, Pei-Lin Shao, Yung-Lung Chen, Sarah Chua, Han-Tan Chai, Yi-Ling Chen, Tien-Hung Huang, Hon-Kan Yip, Mel S Lee
This study tested the hypothesis that combined therapy with exendin-4 (Ex4) and autologous adipose-derived mesenchymal stem cells (ADMSCs) was superior to either alone for protecting renal function against acute kidney ischemia-reperfusion (IR; 40-min ischemia/27-h reperfusion) injury when complicated by sepsis syndrome (SS; by cecal-ligation-puncture). Adult-male Sprague-Dawley rats (n=40) were equally divided into group 1 (sham-control), group 2 (IR-SS), group 3 (IR-SS + Ex4, 10 μg/kg subcutaneously 30 min after reperfusion and daily for 3 days), group 4 [IR-SS + ADMSC (1...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28804459/metabolic-variability-of-a-multispecies-probiotic-preparation-impacts-on-the-anti-inflammatory-activity
#8
Michele Biagioli, Luca Laghi, Adriana Carino, Sabrina Cipriani, Eleonora Distrutti, Silvia Marchianò, Carola Parolin, Paolo Scarpelli, Beatrice Vitali, Stefano Fiorucci
Background: In addition to strain taxonomy, the ability of probiotics to confer beneficial effects on the host rely on a number of additional factors including epigenetic modulation of bacterial genes leading to metabolic variability and might impact on probiotic functionality. Aims: To investigate metabolism and functionality of two different batches of a probiotic blend commercialized under the same name in Europe in models of intestinal inflammation. Methods: Boxes of VSL#3, a probiotic mixture used in the treatment of pouchitis, were obtained from pharmacies in UK subjected to metabolomic analysis and their functionality tested in mice rendered colitis by treatment with DSS or TNBS...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28803254/helicobacter-pylori-cytotoxin-associated-gene-a-impairs-the-filtration-barrier-function-of-podocytes-via-p38-mapk-signaling-pathway
#9
Man Yang, Ling Wang, Li-Jie Gu, Wei-Jie Yuan
Helicobacter pylori (Hp) specific antigens were found deposited in the glomeruli in some kidney diseases. However, the underlying molecular mechanisms remain to be elucidated. The aim of this study was to investigate the effect of cytotoxin associated gene A protein (CagA), a key virulence factor of Hp, on mouse podocytes. Cells were cultured and treated with recombinant CagA protein. The expression of the tight junction protein ZO-1 and p38 MAPK signaling pathway activation were measured with real-time RT-PCR and western blotting...
August 12, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28801650/regulation-of-the-human-phosphatase-ptpn4-by-the-inter-domain-linker-connecting-the-pdz-and-the-phosphatase-domains
#10
Célia Caillet-Saguy, Angelo Toto, Raphael Guerois, Pierre Maisonneuve, Eva di Silvio, Kristi Sawyer, Stefano Gianni, Nicolas Wolff
Human protein tyrosine phosphatase non-receptor type 4 (PTPN4) has been shown to prevent cell death. The active form of human PTPN4 consists of two globular domains, a PDZ (PSD-95/Dlg/ZO-1) domain and a phosphatase domain, tethered by a flexible linker. Targeting its PDZ domain abrogates this protection and triggers apoptosis. We previously demonstrated that the PDZ domain inhibits the phosphatase activity of PTPN4 and that the mere binding of a PDZ ligand is sufficient to release the catalytic inhibition. We demonstrate here that the linker connecting the PDZ domain and the phosphatase domain is involved in the regulation of the phosphatase activity in both PDZ-related inhibition and PDZ ligand-related activation events...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28790012/mdivi-1-ameliorates-early-brain-injury-after-subarachnoid-hemorrhage-via-the-suppression-of-inflammation-related-blood-brain-barrier-disruption-and-endoplasmic-reticulum-stress-based-apoptosis
#11
Lin-Feng Fan, Ping-You He, Yu-Cong Peng, Qing-Hua Du, Yi-Jun Ma, Jian-Xiang Jin, Hang-Zhe Xu, Jian-Ru Li, Zhi-Jiang Wang, Sheng-Long Cao, Tao Li, Feng Yan, Chi Gu, Lin Wang, Gao Chen
Aberrant modulation of mitochondrial dynamic network, which shifts the balance of fusion and fission towards fission, is involved in brain damage of various neurodegenerative diseases including Parkinson's disease, Huntington's disease and Alzheimer's disease. A recent research has shown that the inhibition of mitochondrial fission alleviates early brain injury after experimental subarachnoid hemorrhage, however, the underlying molecular mechanisms have remained to be elucidated. This study was undertaken to characterize the effects of the inhibition of dynamin-related protein-1 (Drp1, a dominator of mitochondrial fission) on blood-brain barrier (BBB) disruption and neuronal apoptosis following SAH and the potential mechanisms...
August 5, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28782908/ectodysplasin-a-regulates-epithelial-barrier-function-through-sonic-hedgehog-signalling-pathway
#12
Sanming Li, Jing Zhou, Liying Zhang, Juan Li, Jingwen Yu, Ke Ning, Yangluowa Qu, Hui He, Yongxiong Chen, Peter S Reinach, Chia-Yang Liu, Zuguo Liu, Wei Li
Ectodysplasin A (Eda), a member of the tumour necrosis factor superfamily, plays an important role in ectodermal organ development. An EDA mutation underlies the most common of ectodermal dysplasias, that is X-linked hypohidrotic ectodermal dysplasia (XLHED) in humans. Even though it lacks a developmental function, the role of Eda during the postnatal stage remains elusive. In this study, we found tight junctional proteins ZO-1 and claudin-1 expression is largely reduced in epidermal, corneal and lung epithelia in Eda mutant Tabby mice at different postnatal ages...
August 7, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28780155/oxyresveratrol-improves-tight-junction-integrity-through-the-pkc-and-mapk-signaling-pathways-in-caco-2%C3%A2-cells
#13
HyunA Jo, Dahyun Hwang, Jeong-Keun Kim, Young-Hee Lim
Strengthening intestinal tight junctions (TJ) provides an effective barrier from the external environment and is important for recovery from inflammatory bowel disease. Oxyresveratrol (OXY), an isomer of hydroxylated resveratrol, is isolated from many plants. The aim of this study was to investigate the effect of OXY on intestinal TJ and to elucidate the mechanism underlying the OXY-mediated increase in TJ integrity in human intestinal Caco-2 cells. OXY-treated Caco-2 cell monolayers showed decreased monolayer permeability as evaluated by paracellular transport assay...
August 2, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28777077/a-flexible-thermoresponsive-cell-culture-substrate-for-direct-transfer-of-keratinocyte-cell-sheets
#14
Praveen Wulligundam, Bernadette Madathil, Sajin Raj Rg, Kumary Tv, Anil Kumar Pr
Most cell sheet engineering systems require a support or carrier to handle the harvested cell sheets. In this study, polyethylene terephthalate-based overhead projection transparency sheets (OHPS) were subjected to surface hydrolysis by alkali treatment to increase pliability and hydrophilicity and enable poly(N-isopropylacrylamide-co-glycidylmethacrylate) copolymer (NGMA) coating to impart thermoresponsiveness. NGMA was applied on the modified OHPS by the technique of spin coating using an indigenously designed spin coater...
August 4, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28774775/mir-15a-16-inhibits-tgf-beta3-vegf-signaling-and-increases-retinal-endothelial-cell-barrier-proteins
#15
Eun-Ah Ye, Li Liu, Jena J Steinle
Hyperglycemia is a significant risk factor for diabetic retinopathy and induces multiple biochemical changes, including inflammation and endothelial dysfunction in the retina. Alterations in microRNA expression have been implicated in the pathological responses of diabetic retinopathy and the manipulation of microRNA may provide powerful strategy for therapeutics. Among the predicted targets of miR-15a and -16 are TGF-beta3, SMAD2/3, and VEGF, all of which are known to play a role in vascular endothelial functions...
July 31, 2017: Vision Research
https://www.readbyqxmd.com/read/28771130/indigenous-lactobacilli-strains-of-food-and-human-sources-reverse-enteropathogenic-e-coli-o26-h11-induced-damage-in-intestinal-epithelial-cell-lines-effect-on-redistribution-of-tight-junction-proteins
#16
Ruchi Jariwala, Hemanti Mandal, Tamishraha Bagchi
The aim of the study was to investigate the neutralizing effect of lactobacilli isolated from indigenous food and human sources on enteropathogenic Escherichia coli (EPEC) O26 : H11-induced epithelial barrier dysfunction in vitro. This was assessed by transepithelial electrical resistance (TEER) and permeability assays using intestinal cell lines, HT-29 and Caco-2. Furthermore, the expression and distribution of tight junction (TJ) proteins were analysed by qRT-PCR and immunofluorescence assay, respectively...
August 4, 2017: Microbiology
https://www.readbyqxmd.com/read/28770828/sesamin-alleviates-blood-brain-barrier-disruption-in-mice-with-experimental-traumatic-brain-injury
#17
Ying-Liang Liu, Zhi-Ming Xu, Guo-Yuan Yang, Dian-Xu Yang, Jun Ding, Hao Chen, Fang Yuan, Heng-Li Tian
Sesamin, a major lignan of sesame oil, was reported to have neuroprotective effects in several brain injury models. However, its protective action in maintaining blood-brain barrier (BBB) integrity has not been studied. In this study we investigated the effects of sesamin on the BBB in a mouse model of traumatic brain injury (TBI) and explored the underlying mechanisms. Adult male C57BL/6 mice were subjected to a controlled cortical impact (CCI) injury and then received sesamin (30 mg·kg(-1)·d(-1), ip). The mice were euthanized on the 1(st) and 3(rd) days after CCI injury and samples were collected for analysis...
August 3, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28769101/allostimulatory-capacity-of-conditionally-immortalized-proximal-tubule-cell-lines-for-bioartificial-kidney-application
#18
Milos Mihajlovic, Lambertus P van den Heuvel, Joost G Hoenderop, Jitske Jansen, Martijn J Wilmer, Annemarie J F Westheim, Wil A Allebes, Dimitrios Stamatialis, Luuk B Hilbrands, Rosalinde Masereeuw
Novel renal replacement therapies, such as a bioartificial kidney (BAK), are needed to improve current hemodialysis treatment of end-stage renal disease (ESRD) patients. As BAK applications may reveal safety concerns, we assessed the alloimmunization and related safety aspects of readily available conditionally immortalized human proximal tubule epithelial cell (ciPTEC) lines to be used in BAK. Two ciPTEC lines, originally derived from urine and kidney tissue, were characterized for the expression and secretion of relevant molecules involved in alloimmunization and inflammatory responses, such as HLA class-I, HLA-DR, CD40, CD80, CD86, as wells as soluble HLA class I and proinflammatory cytokines (IL-6, IL-8 and TNF-α)...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765694/lactobacillus-acidophilus-alleviates-pouchitis-after-ileal-pouch-anal-anastomosis-in-rats
#19
Yan-Yan Xu, Ying-Ying Zhang, An-Qi He, Kai-Yu Li, Sen-Yang Gao, Gang Liu
AIM: To assess the therapeutic potential of Lactobacillus acidophilus (LA) for the treatment of pouchitis in a rat model. METHODS: Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium (DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention (NI), normal saline (NS, 3 mL/d normal saline for 7 d), and LA (3 mL/d LA at 1× 10(10) colony-forming units for 7 d)...
July 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28762325/activation-of-vimentin-is-critical-to-promote-a-metastatic-potential-of-cholangiocarcinoma-cells
#20
Waraporn Saentaweesuk, Norie Araki, Kulthida Vaeteewoottacharn, Atit Silsirivanit, Wunchana Seubwai, Chutima Talabnin, Kanha Muisuk, Banchob Sripa, Sopit Wongkham, Seiji Okada, Chaisiri Wongkham
Cholangiocarcinoma (CCA) is a highly metastatic tumor, of which the majority of the patients have short survival due tono effective treatment. Hence, a better understanding regarding CCA metastasis may provide an opportunity to improve the strategies for treatment. A comparison study between the highly metastatic cells and their parental cells is an approach to uncover the molecular mechanisms underlying the metastatic process. In the present study, a lung metastatic CCA cell line, KKU-214L5 was established by the in vivo selection of the tail-vein injected mouse model...
July 25, 2017: Oncology Research
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