keyword
MENU ▼
Read by QxMD icon Read
search

ZO-1

keyword
https://www.readbyqxmd.com/read/28211084/endothelial-cells-biophysical-biochemical-and-chromosomal-aberrancies-in-high-glucose-condition-within-the-diabetic-range
#1
Aysa Rezabakhsh, Elahe Nabat, Mina Yousefi, Soheila Montazersaheb, Omid Cheraghi, Amir Mehdizadeh, Farzaneh Fathi, Ali Akbar Movassaghpour, Nasrin Maleki-Dizaji, Reza Rahbarghazi, Alireza Garjani
To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia-induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells-especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high-glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours...
February 17, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28206713/effect-of-heme-oxygenase-1-transduced-bone-marrow-mesenchymal-stem-cells-on-damaged-intestinal-epithelial-cells-in-vitro
#2
Yi Cao, Ben-Juan Wu, Wei-Ping Zheng, Ming-Li Yin, Tao Liu, Hong-Li Song
In this study we explored the effects of mesenchymal stem cells (MSCs) from bone marrow overexpressing heme oxygenase-1 (HO-1) on the damaged human intestinal epithelial barrier in vitro. Rat MSCs were isolated from bone marrow and transduced with rat HO-1 recombinant adenovirus (HO-MSCs) for stable expression of HO-1. Colorectal adenocarinoma 2 (Caco2) cells were treated with tumor necrosis factor-α (TNF-α) to establish a damaged colon epithelial model. Damaged Caco2 were cocultured with MSCs, Ad-MSCs, Ad-HO + MSCs or HO-MSCs mRNA and protein expression of Zona occludens-1 (ZO-1) and human HO-1, and the release of cytokines were measured...
February 16, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28199886/construction-of-tissue-engineered-full-thickness-cornea-substitute-using-limbal-epithelial-cell-like-and-corneal-endothelial-cell-like-cells-derived-from-human-embryonic-stem-cells
#3
Canwei Zhang, Liqun Du, Peng Sun, Lin Shen, Jing Zhu, Kunpeng Pang, Xinyi Wu
The aim of this study was to construct a full-thickness artificial cornea substitute in vitro by coculturing limbal epithelial cell-like (LEC-like) cells and corneal endothelial cell-like (CEC-like) cells derived from human embryonic stem cells (hESCs) on APCM scaffold. A 400 μm thickness, 11 mm diameter APCM lamella containing Bowman's membrane was prepared as the scaffold using trephine and a special apparatus made by ourselves. LEC-like cells and CEC-like cells, derived from hESCs as our previously described, were cocultured on the scaffold using a special insert of 24-well plates that enabled seeding both sides of the scaffold...
February 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28198394/cockroach-protease-allergen-induces-allergic-airway-inflammation-via-epithelial-cell-activation
#4
Sagar L Kale, Komal Agrawal, Shailendra Nath Gaur, Naveen Arora
Protease allergens are known to enhance allergic inflammation but their exact role in initiation of allergic reactions at mucosal surfaces still remains elusive. This study was aimed at deciphering the role of serine protease activity of Per a 10, a major cockroach allergen in initiation of allergic inflammation at mucosal surfaces. We demonstrate that Per a 10 increases epithelial permeability by disruption of tight junction proteins, ZO-1 and occludin, and enhances the migration of Monocyte derived dendritic cell precursors towards epithelial layer as exhibited by trans-well studies...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195240/mir302-regulates-snai1-expression-to-control-mesangial-cell-plasticity
#5
L De Chiara, D Andrews, A Watson, G Oliviero, G Cagney, J Crean
Cell fate decisions are controlled by the interplay of transcription factors and epigenetic modifiers, which together determine cellular identity. Here we elaborate on the role of miR302 in the regulation of cell plasticity. Overexpression of miR302 effected silencing of the TGFβ type II receptor and facilitated plasticity in a manner distinct from pluripotency, characterized by increased expression of Snail. miR302 overexpressing mesangial cells also exhibited enhanced expression of EZH2 coincident with Snail upregulation...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28193579/zinc-enhances-intestinal-epithelial-barrier-function-through-the-pi3k-akt-mtor-signaling-pathway-in-caco-2-cells
#6
Yuxin Shao, Patricia G Wolf, Shuangshuang Guo, Yuming Guo, H Rex Gaskins, Bingkun Zhang
Zinc plays an important role in maintaining intestinal barrier function as well as modulating cellular signaling recognition and protein kinase activities. The phosphatidylinositol 3-kinase (PI3K) cascade has been demonstrated to affect intercellular integrity and tight junction (TJ) proteins. The current study investigated the hypothesis that zinc regulates intestinal intercellular junction integrity through the PI3K/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. A transwell model of Caco-2 cell was incubated with 0, 50 and 100 μM of zinc at various time points...
January 31, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28193461/gill-structural-integrity-changes-in-fish-deficient-or-excessive-in-dietary-isoleucine-towards-the-modulation-of-tight-junction-protein-inflammation-apoptosis-and-antioxidant-defense-via-nf-%C3%AE%C2%BAb-tor-and-nrf2-signaling-pathways
#7
Lin Feng, Lu Gan, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Ling Tang, Sheng-Yao Kuang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study firstly aimed to test the impact of dietary isoleucine (Ile) on tight junction protein, inflammation, apoptosis, antioxidant defense and related signaling molecule gene expression in the gill of fish. Young grass carp (Ctenopharyngodon idella) (weighing 256.8 ± 3.5 g) were fed six diets containing graded levels of Ile, namely, 3.8, 6.6, 9.3, 12.5, 15.2 and 18.5 g/kg diet for 8 weeks. The results firstly revealed that Ile deficiency down-regulated the mRNA expressions of claudin-3, claudin-b, claudin-c, occludin and zonula occludens-1 (ZO-1) and up-regulated the mRNA expression of claudin-12, which led to the intercellular structure damage of fish gill...
February 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28191699/ndrg1-is-important-to-maintain-the-integrity-of-airway-epithelial-barrier-through-claudin-9-expression
#8
Yasuhiro Gon, Shuichiro Maruoka, Hiroyuki Kishi, Yutaka Kozu, Kuroda Kazumichi, Yasuyuki Nomura, Ikuko Takeshita, Takeshi Oshima, Shu Hashimoto
Impairment of epithelial barrier integrity caused by environmental triggers is associated with the pathogenesis of airway inflammation. Using human airway epithelial cells, we attempted to identify molecule(s) that promote airway epithelial barrier integrity. Microarray analyses were conducted using the Affimetrix human whole genome gene chip, and we identified the N-myc downstream-regulated gene 1 (NDRG1) gene, which was induced during the development of the epithelial cell barrier. Immunohistochemical analysis revealed strong NDRG1 expression in ciliated epithelial cells in nasal tissues sampled from patients with chronic rhinosinusitis (CRS), and the low expression of NDRG1 was observed in goblet cells or damaged epithelial cells...
February 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28188749/loss-of-par-3-protein-expression-is-associated-with-invasion-lymph-node-metastasis-and-poor-survival-in-esophageal-squamous-cell-carcinoma
#9
Tomoko Kitaichi, Kohichiroh Yasui, Yasuyuki Gen, Osamu Dohi, Naoto Iwai, Akira Tomie, Nobuhisa Yamada, Kei Terasaki, Atsushi Umemura, Taichiro Nishikawa, Kanji Yamaguchi, Michihisa Moriguchi, Yoshio Sumida, Hironori Mitsuyoshi, Yuji Naito, Yoh Zen, Yoshito Itoh
Disrupted cell polarity is a feature of epithelial cancers. The partitioning defective 3 (PAR-3) protein, a key component of the PAR complex that regulates the polarization of cells, is involved in tight junction formation at epithelial cell-cell contacts. Our previous study detected a homozygous deletion of the PAR-3 gene in esophageal squamous cell carcinoma (ESCC) cell lines and frequent copy number loss of the PAR-3 gene in primary ESCC. Here, we aimed to investigate the clinicopathological relevance of altered expression of the PAR-3 protein in primary ESCC...
February 8, 2017: Human Pathology
https://www.readbyqxmd.com/read/28188396/how-to-obtain-good-morphology-and-antigen-detection-in-the-same-tissue-section
#10
Daša Zupančič, Marjeta Terčelj, Bojan Štrus, Peter Veranič
Most human and animal biopsy samples are routinely embedded in paraffin since this enables the pathologist or researcher to obtain excellent morphology and simplifies storage. Nevertheless, in many cases, the antigen of interest cannot be detected in paraffin section. The alternative available for good immunohistochemistry is preparation of cryosections, which usually provide decent antigen preservation and are frequently used for immunofluorescence. However, cryosections often do not provide efficient morphological details of tissues and cells for pathologic evaluation...
February 10, 2017: Protoplasma
https://www.readbyqxmd.com/read/28185956/long-non-coding-rna-neat1-regulates-permeability-of-the-blood-tumor-barrier-via-mir-181d-5p-mediated-expression-changes-in-zo-1-occludin-and-claudin-5
#11
Junqing Guo, Heng Cai, Jun Ma, Xiaobai Liu, Yunhui Liu, Jian Zheng, Zhongyou Que, Wei Gong, Yana Gao, Wei Tao, Yixue Xue
The blood-tumor barrier (BTB) constitutes an efficient organization of tight junctions that limits the delivery of chemotherapeutic drugs to brain tumor tissues and impacts the treatment of glioma. Long non-coding RNAs (lncRNAs) are non-protein coding RNAs regulating gene expression, some lncRNAs play a crucial role in BTB permeability. However, the function of lncRNAs in BTB permeability is still largely unclear. Here, we have identified lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1), was remarkably up-regulated in glioma endothelial cells (GECs) obtained from an in vitro BTB model...
February 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28182318/molecular-and-structural-changes-related-to-hepatitis-e-virus-hev-antigen-and-its-expression-in-testis-inducing-apoptosis-in-mongolian-gerbil-model
#12
Majid Hussain Soomro, Ruihan Shi, Ruiping She, Yifei Yang, Tongtong Wang, Qiaoxing Wu, Heng Li, Wenzhuo Hao
Hepatitis E virus (HEV) infection has been associated with a wide range of extrahepatic manifestations, so this study was designed to examine the effect and role of HEV on structural and molecular changes in the testicular tissues of Mongolian gerbils experimentally infected with swine HEV. HEV RNA was first detected in testis at 14 day post inoculation and reached a peak between 28 to 42 days later with viral load between 3.12 logs g-¹ to 6.23 logs g-¹ by PCR assays. Microscopic changes showed vacuolation, sloughing of germ cells, formation of multinuclear giant cells, degeneration, necrosis of tubules and damaged blood-testis barrier was observed through transmission electron microscopy...
February 9, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28174772/collagen-peptides-ameliorate-intestinal-epithelial-barrier-dysfunction-in-immunostimulatory-caco-2-cell-monolayers-via-enhancing-tight-junctions
#13
Qianru Chen, Oliver Chen, Isabela M Martins, Hu Hou, Xue Zhao, Jeffrey B Blumberg, Bafang Li
Dysfunction of the intestinal barrier plays a key role in the pathogenesis of inflammatory bowel disease (IBD) and multiple organ failure. The effect of Alaska pollock skin-derived collagen and its 3 tryptic hydrolytic fractions, HCP (6 kDa retentate), MCP (3 kDa retentate) and LCP (3 kDa permeate) on TNF-α induced barrier dysfunction was investigated in Caco-2 cell monolayers. TNF-α induced barrier dysfunction was significantly attenuated by the collagen and its peptide fractions, especially LCP, compared to TNF-α treated controls (P < 0...
February 8, 2017: Food & Function
https://www.readbyqxmd.com/read/28170537/adenosine-deaminase-2-induced-hyperpermeability-in-human-retinal-vascular-endothelial-cells-is-suppressed-by-microrna-146b-3p
#14
Yara A Samra, Heba M Saleh, Khaled A Hussein, Nehal M Elsherbiny, Ahmed S Ibrahim, Khaled Elmasry, Sadanand Fulzele, Mamdouh M El-Shishtawy, Laila A Eissa, Mohamed Al-Shabrawey, Gregory I Liou
Purpose: We recently demonstrated that adenosine deaminase-2 (ADA2) contributes to diabetic retinopathy (DR) via up-regulating the production of inflammatory cytokines in macrophages. Also, microRNA (miR)-146b-3p has the ability to inhibit ADA2. The goal of this study was to investigate the potential role of ADA2 and therapeutic benefit of miR-146b-3p in retinal inflammation and endothelial barrier dysfunction during diabetes. Methods: Adenosine deaminase-2 activity was determined by colorimetric method in diabetic human vitreous...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28169530/intranasal-pretreatment-with-z-ligustilide-the-main-volatile-component-of-rhizoma-chuanxiong-confers-prophylaxis-against-cerebral-ischemia-via-nrf2-and-hsp70-signaling-pathways
#15
Juan Li, Jie Yu, Hui Ma, Na Yang, Li Li, Ding-Ding Zheng, Ming-Xia Wu, Zhi-Long Zhao, Hong-Yi Qi
Z-Ligustilide (Z-LIG) is a major component in Rhizoma Chuanxiong, which has been traditionally used as a health food supplement for the prevention of cerebrovascular disease in China. This study investigates the ability of intranasal Z-LIG pretreatment to enhance protection against neuronal damage in rats with middle cerebral artery occlusion (MCAO) and the role of cellular stress response mechanisms Nrf2 and HSP70. Z-LIG significantly mitigated infarct volume, neurological dysfunction, blood-brain barrier disruption, and brain edema (p < 0...
February 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28158914/effect-of-porcine-glucagon-like-peptides-2-on-tight-junction-in-glp-2r%C3%A2-%C3%A2-ipec-j2-cell-through-the-pi3-k-akt-mtor-p70-s6k-signalling-pathway
#16
K K Qi, Y Q Sun, J Wan, B Deng, X M Men, J Wu, Z W Xu
Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and determined the molecular mechanisms of the protective effects of GLP-2-mediated tight junctions (TJ) in GLP-2R (+) cell line. (i) Immunohistochemistry results showed that GLP-2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets...
February 3, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28153728/mir-34c-and-plncrna1-mediated-the-function-of-intestinal-epithelial-barrier-by-regulating-tight-junction-proteins-in-inflammatory-bowel-disease
#17
Tanzhou Chen, Haibo Xue, Ruoyang Lin, Zhiming Huang
BACKGROUND: Inflammatory bowel disease (IBD) is originated from uncontrolled inflammation, and desired methods for IBD therapy remains the main difficult. The network comprised with miRNA and lncRNA has been verified to play an important role on diverse human diseases. In this study, we demonstrated the role of miR-34c and lncRNA PlncRNA1 on the function of intestinal barrier. METHODS: Intestinal epithelial barrier model was constructed based on normal intestinal epithelial cell line Caco-2...
January 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28151586/nuclear-erythroid-2-related-factor-2-activation-inhibits-house-dust-mite-induced-sinonasal-epithelial-cell-barrier-dysfunction
#18
Nyall R London, Anuj Tharakan, Andrew P Lane, Shyam Biswal, Murugappan Ramanathan
BACKGROUND: Dysregulated sinonasal epithelial cell (SNEC) barrier function has been proposed to contribute to the pathogenesis of sinonasal inflammatory conditions such as allergic rhinitis (AR) and chronic rhinosinusitis (CRS). Allergens such as house dust mite (HDM) have been reported to disrupt SNEC barrier integrity. We have recently identified nuclear erythroid 2-related factor 2 (Nrf2) activation via sulforaphane (SFN) stimulation to stabilize SNEC barrier function. The purpose of this study was to explore whether Nrf2 activation could ameliorate HDM-induced SNEC barrier dysfunction...
February 2, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28146239/combined-pi3k-akt-and-smad2-activation-promotes-corneal-endothelial-cell-proliferation
#19
Alfonso L Sabater, Enrique J Andreu, María García-Guzmán, Tania López, Gloria Abizanda, Victor L Perez, Javier Moreno-Montañés, Felipe Prósper
Purpose: The purpose of this study was to develop a culture method for expansion of corneal endothelial cells (CEC) based on the combined activation of PI3K/Akt and Smad2. Methods: Morphology, proliferation, and migration of cultured rabbit and nonhuman primate CEC were examined in the presence of the PI3K/Akt activators IGF-1 and heregulin beta in combination with the Smad2 activator activin A. Phenotypic characterization of CEC was performed at the RNA and protein levels...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28142299/tgf-%C3%AE-1-protects-intestinal-integrity-and-influences-smads-and-mapk-signal-pathways-in-ipec-j2-after-tnf-%C3%AE-challenge
#20
Kan Xiao, Shuting Cao, Lefei Jiao, Zehe Song, Jianjun Lu, Caihong Hu
The aim of this study was to investigate the protective effects of TGF-β1 on intestinal epithelial barrier, as well as canonical Smad and MAPK signal pathways involved in these protection processes by a IPEC-J2 model stimulated with TNF-α. IPEC-J2 monolayers were treated without or with TNF-α in the absence or presence of TGF-β1. The results showed that TGF-β1 pretreatment ameliorated TNF-α-induced intestinal epithelial barrier disturbances as indicated by decrease of transepithelial electrical resistance (TER) and increase of paracellular permeability...
January 1, 2017: Innate Immunity
keyword
keyword
97497
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"