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https://www.readbyqxmd.com/read/29110513/ankaferd-influences-mrna-expression-of-iron-regulated-genes-during-iron-deficiency-anemia
#1
Afife Gulec, Sukru Gulec
Ankaferd Blood Stopper (ABS) comprises a mixture of plants and stops bleeding via forming a protein network by erythroid aggregation. Bleeding causes reduction of iron levels in body. It has been indicated that ABS contains significant amount of iron. Thus, we investigated the biological activity of ABS-derived iron on iron-regulated genes during iron-deficiency anemia (IDA). IDA We selected Caco-2 and HepG2 cell lines as in vitro models of human intestine and liver, respectively. Iron deficiency anemia was induced by deferoxamine...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28965584/identification-of-eight-copper-cu-uptake-related-genes-from-yellow-catfish-pelteobagrus-fulvidraco-and-their-tissue-expression-and-transcriptional-responses-to-dietborne-cu-exposure
#2
Jie Cheng, Zhi Luo, Guang-Hui Chen, Chuan-Chuan Wei, Mei-Qin Zhuo
The present working hypothesis is that absorption of dietary Cu is related to mRNA expressions of genes involved in Cu uptake and transport of the intestine in fish. To this end, the full-length cDNA sequences of eight Cu uptake related genes, including two isoforms of copper transporter genes (ctr1 and ctr2), three copper chaperone genes (atox1, ccs and cox17), two Cu-ATPase genes (atp7a and atp7b) and divalent metal ion transporter 1 (dmt1), were cloned and characterized in yellow catfish P. fulvidraco, respectively...
December 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/28955775/dynamics-of-absorption-metabolism-and-excretion-of-5-aminolevulinic-acid-in-human-intestinal-caco-2-cells
#3
Kei Saito, Tohru Fujiwara, Urara Ota, Shunsuke Hatta, Satoshi Ichikawa, Masahiro Kobayashi, Yoko Okitsu, Noriko Fukuhara, Yasushi Onishi, Masahiro Ishizuka, Tohru Tanaka, Hideo Harigae
5-Aminolevulinic acid (ALA) is a precursor for the biosynthesis of porphyrins and heme. Although the oral administration of ALA has been widely applied in clinical settings, the dynamics of its absorption, metabolism, and excretion within enterocytes remain unknown. In this study, after enterocytic differentiation, Caco-2 cells were incubated with 200 µM ALA and/or 100 µM sodium ferrous citrate (SFC) for up to 72 h. Both ALA and the combination of ALA and SFC promoted the synthesis of heme, without affecting the expression of genes involved in intestinal iron transport, such as DMT1 and FPN...
September 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28937159/duodenal-cytochrome-b-cybrd1-ferric-reductase-functional-studies-in-cells
#4
F Schlottmann, M Vera-Aviles, G O Latunde-Dada
Dietary non-heme ferric iron is reduced by the ferric reductase enzyme, duodenal cytochrome b (Dcytb), before absorption by the divalent metal transporter 1 (DMT1). A single nucleotide polymorphism (SNP rs10455 mutant) that is located in the last exon of the Dcytb gene was reported in C282Y haemochromatosis HFE subjects. The present work therefore investigated the phenotype of this mutant Dcytb in Chinese hamster ovary (CHO) cells. These cultured cells were transfected with either wild type (WT) or the SNP vector plasmids of Dcytb...
October 18, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28874056/ceruloplasmin-a-potential-therapeutic-agent-for-alzheimer-s-disease
#5
Ya-Shuo Zhao, Li-Hong Zhang, Pan-Pan Yu, Yu-Jing Gou, Jing Zhao, Lin-Hao You, Zhan-You Wang, Xin Zheng, Liang-Jun Yan, Peng Yu, Yan-Zhong Chang
AIMS: Ceruloplasmin (CP), a ferrous oxidase enzyme, plays an important role in regulating iron metabolism and redox reactions. Previous studies showed that CP deficiency contributes to Parkinson's disease by increasing iron accumulation and oxidative stress in the substantia nigra. However, the role of CP in Alzheimer's disease (AD) is unclear. We hypothesized that the lack of CP gene expression would affect the pathogenesis and damage of AD by promoting abnormal iron levels and oxidative stress...
October 17, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28827515/microrna-expression-analysis-in-serum-of-patients-with-congenital-hemochromatosis-and-age-related-macular-degeneration-amd
#6
Maciej Szemraj, Katarzyna Oszajca, Janusz Szemraj, Piotr Jurowski
BACKGROUND Congenital hemochromatosis is a disorder caused by mutations of genes involved in iron metabolism, leading to increased levels of iron concentration in tissues and serum. High concentrations of iron can lead to the development of AMD. The aim of this study was to analyze circulating miRNAs in the serum of congenital hemochromatosis patients with AMD and their correlation with the expression of genes involved in iron metabolism. MATERIAL AND METHODS Peripheral blood monolayer cells and serum were obtained from patients with congenital hemochromatosis, congenital hemochromatosis and AMD, AMD patients without congenital hemochromatosis, and healthy controls...
August 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28694024/iron-metabolism-state-of-the-art
#7
R Daher, Z Karim
Iron homeostasis relies on the amount of its absorption by the intestine and its release from storage sites, the macrophages. Iron homeostasis is also dependent on the amount of iron used for the erythropoiesis. Hepcidin, which is synthesized predominantly by the liver, is the main regulator of iron metabolism. Hepcidin reduces serum iron by inhibiting the iron exporter, ferroportin expressed both tissues, the intestine and the macrophages. In addition, in the enterocytes, hepcidin inhibits the iron influx by acting on the apical transporter, DMT1...
September 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28673786/diurnal-variations-in-iron-concentrations-and-expression-of-genes-involved-in-iron-absorption-and-metabolism-in-pigs
#8
Yiming Zhang, Dan Wan, Xihong Zhou, Ciming Long, Xin Wu, Lan Li, Liuqin He, Pan Huang, Shuai Chen, Bie Tan, Yulong Yin
Diurnal variations in serum iron levels have been well documented in clinical studies, and serum iron is an important diagnostic index for iron-deficiency anemia. However, the underlying mechanism of dynamic iron regulation in response to the circadian rhythm is still unclear. In this study, we investigated daily variations in iron status in the plasma and liver of pigs. The transcripts encoding key factors involved in iron uptake and homeostasis were evaluated. The results showed that iron levels in the plasma and liver exhibited diurnal rhythms...
September 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28464091/effect-of-iron-source-on-iron-absorption-and-gene-expression-of-iron-transporters-in-the-ligated-duodenal-loops-of-broilers
#9
L Y Zhang, X F Li, X D Liao, L Y Zhang, L Lu, X G Luo
This experiment was conducted to investigate the effect of iron source on Fe absorption and the gene expression of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1) in the ligated duodenal loops of broilers. The in situ ligated duodenal loops from Fe-deficient broiler chicks (28-d-old) were perfused with Fe solutions containing 0 to 14.33 mmol Fe/L from 1 of the following: Fe sulfate (FeSO∙7HO), Fe methionine with weak chelation strength (Fe-Met W; chelation strength is expressed as quotient of formation [Q] value, Q = 1...
April 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28338971/progressive-silencing-of-the-zinc-transporter-zip8-slc39a8-in-chronic-cadmium-exposed-lung-epithelial-cells
#10
Yangmin Gao, Yanming Xu, Dandan Wu, Feiyuan Yu, Lei Yang, Yue Yao, Zhanling Liang, Andy T Y Lau
Cadmium (Cd), a non-essential metal, stealthily enters the cells by utilizing the essential metal importing pathways. The zinc transporters Zip8, Zip14, and divalent metal transporter 1 (Dmt1) are now emerging as several important metal transporters involved in cellular Cd incorporation and their expressions have been shown to be down-regulated in several Cd-resistant (CdR) cell lines, however, the involvement of these transporters during the development of Cd-resistance in lung cells is unclear. In this study, we therefore check the expression of these metal transporters in our previously established rat lung epithelial cells (LECs) and show that the level of Zip8 is progressively silenced when LECs are adapted to increasing concentrations of CdCl2 (from 1 to 20 μM)...
May 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28335378/bioavailability-of-microencapsulated-iron-from-fortified-bread-assessed-using-piglet-model
#11
Malgorzata A Bryszewska, Luca Laghi, Augusta Zannoni, Andrea Gianotti, Francesca Barone, Danielle L Taneyo Saa, Maria L Bacci, Domenico Ventrella, Monica Forni
The aim of this study was to investigate the influence of oral iron supplementation, in the form of fortified breads, on the growth performance, health, iron status parameters, and fecal metabolome of anemic piglets. A study was conducted on 24 hybrid (Large White × Landrace × Duroc) piglets. From day 44, the post-natal 12 piglets were supplemented with 100 g of one of two experimental breads, each fortified with 21 mg of ferrous sulphate, either encapsulated or not. After one week of oral supplementation, hematological parameters (hematocrit value, hemoglobin, and red blood cells) showed statistically significant differences (p ≤ 0...
March 13, 2017: Nutrients
https://www.readbyqxmd.com/read/28270217/dcytb-is-a-predictor-of-outcome-in-breast-cancer-that-functions-via-iron-independent-mechanisms
#12
David J Lemler, Miranda L Lynch, Lia Tesfay, Zhiyong Deng, Bibbin T Paul, Xiaohong Wang, Poornima Hegde, David H Manz, Suzy V Torti, Frank M Torti
BACKGROUND: Duodenal cytochrome b (DCYTB) is a ferrireductase that functions together with divalent metal transporter 1 (DMT1) to mediate dietary iron reduction and uptake in the duodenum. DCYTB is also a member of a 16-gene iron regulatory gene signature (IRGS) that predicts metastasis-free survival in breast cancer patients. To better understand the relationship between DCYTB and breast cancer, we explored in detail the prognostic significance and molecular function of DCYTB in breast cancer...
March 7, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28261264/ferrous-iron-up-regulation-in-fibroblasts-of-patients-with-beta-propeller-protein-associated-neurodegeneration-bpan
#13
Rosaria Ingrassia, Maurizio Memo, Barbara Garavaglia
Mutations in WDR45 gene, coding for a beta-propeller protein, have been found in patients affected by Neurodegeneration with Brain Iron Accumulation, NBIA5 (also known as BPAN). BPAN is a movement disorder with Non Transferrin Bound Iron (NTBI) accumulation in the basal ganglia as common hallmark between NBIA classes (Hayflick et al., 2013). WDR45 has been predicted to have a role in autophagy, while the impairment of iron metabolism in the different NBIA subclasses has not currently been clarified. We found the up-regulation of the ferrous iron transporter (-)IRE/Divalent Metal Transporter1 and down-regulation of Transferrin receptor in the fibroblasts of two BPAN affected patients with splicing mutations 235+1G>A (BPAN1) and 517_519ΔVal 173 (BPAN2)...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28159226/the-effect-of-amino-acid-deprivation-on-the-transfer-of-iron-through-caco-2-cell-monolayers
#14
Guenievre Roussel, Valerie Stevens, Sarah Cottin, Harry J McArdle
Iron (Fe) metabolism is modified by many nutritional factors. Amino acids (AA) play a central role in various biological processes, such as protein synthesis and energy supply. However, the influence of AA status on iron metabolism has not been investigated. Here, we test whether AA alters iron metabolism in an intestinal cell model. Both Fe uptake and transfer across the cell monolayer were significantly increased by non-essential AA deficiency (both p<0.001) while only Fe transfer was increased by essential AA deficiency (p<0...
March 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27991585/hephaestin-and-ceruloplasmin-facilitate-iron-metabolism-in-the-mouse-kidney
#15
Bo Jiang, Guohao Liu, Jiashuo Zheng, Mengxia Chen, Zaitunamu Maimaitiming, Min Chen, Shunli Liu, Ruiwei Jiang, Brie K Fuqua, Joshua L Dunaief, Chris D Vulpe, Gregory J Anderson, Hongwei Wang, Huijun Chen
Multicopper ferroxidases (MCFs) play an important role in cellular iron homeostasis. However, the role of MCFs in renal metabolism remains unclear. We used Hephaestin (Heph) and Ceruloplasmin (Cp) single or double (Heph/Cp) knockout (KO) mice to study the roles of MCFs in the kidney. Renal iron levels and the expression of iron metabolism genes were examined. The non-heme iron content both in the renal cortex and medulla of Heph/Cp KO mice was significantly increased. Perls' Prussian blue staining showed iron accumulation on the apical side of renal tubular cells in Heph/Cp KO mice...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27980776/macrophagic-control-of-the-response-to-uropathogenic-e-coli-infection-by-regulation-of-iron-retention-in-an-il-6-dependent-manner
#16
Nana Owusu-Boaitey, Kyle A Bauckman, Tingxuan Zhang, Indira U Mysorekar
INTRODUCTION: Uropathogenic Escherichia coli (UPEC), the causative agent of over 85% of urinary tract infections (UTIs), elaborate a number of siderophores to chelate iron from the host. On the other hand, the host immune imperative is to limit the availability of iron to the bacteria. Little is known regarding the mechanisms underlying this host-iron-UPEC interaction. Our objective was to determine whether macrophages, in response to UPEC infection, retain extracellular siderophore-bound and free iron, thus limiting the ability of UPEC to access iron...
December 2016: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/27729173/intestinal-expression-of-genes-implicated-in-iron-absorption-and-their-regulation-by-hepcidin
#17
Gaetano Bergamaschi, Antonio Di Sabatino, Alessandra Pasini, Cristina Ubezio, Filippo Costanzo, Davide Grataroli, Michela Masotti, Costanza Alvisi, Gino R Corazza
BACKGROUND & AIMS: Through inhibition of iron absorption and iron mobilization from tissue stores, hepcidin exerts a negative control on iron homeostasis. Hepcidin, in fact, promotes the degradation of ferroportin (Fpn1), the iron exporter molecule expressed on the membrane of hepatocytes and macrophages, thus preventing iron release from cells to plasma. Hepcidin effects on enterocytes, however, are less clear. Aim of the present study was to further investigate the regulation of iron absorption by hepcidin...
September 30, 2016: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27714044/knockdown-of-copper-transporting-atpase-1-atp7a-impairs-iron-flux-in-fully-differentiated-rat-iec-6-and-human-caco-2-intestinal-epithelial-cells
#18
Jung-Heun Ha, Caglar Doguer, James F Collins
Intestinal iron absorption is highly regulated since no mechanism for iron excretion exists. We previously demonstrated that expression of an intestinal copper transporter (Atp7a) increases in parallel with genes encoding iron transporters in the rat duodenal epithelium during iron deprivation (Am. J. Physiol.: Gastrointest. Liver Physiol., 2005, 288, G964-G971). This led us to postulate that Atp7a may influence intestinal iron flux. Therefore, to test the hypothesis that Atp7a is required for optimal iron transport, we silenced Atp7a in rat IEC-6 and human Caco-2 cells...
September 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27647474/cadmium-induced-oxidative-damages-in-the-human-bjab-cells-correlate-with-changes-in-intracellular-trace-elements-levels-and-zinc-transporters-expression
#19
Saïd Nemmiche, Pascale Guiraud
Cadmium (Cd), a potent toxic heavy metal, is a widespread environmental contaminant. Its cellular traffic via pathways dedicated to transition metals contributes to the toxicity mechanisms. Zinc (Zn) homeostasis is complex, involving both zinc importers (Zip) and zinc exporters (ZnT). Cellular signal transduction pathways are influenced by Zn and redox status of the cell. The aim of the present study is to examine if the accumulation of Cd in the human lymphocyte B cell line BJAB and its capacity to promote oxidative stress and adverse effects could result from changes in the mRNA expression pattern of Zn transporters and metallothioneins...
December 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27551308/vitamin-a-deficiency-modulates-iron-metabolism-independent-of-hemojuvelin-hfe2-and-bone-morphogenetic-protein-6-bmp6-transcript-levels
#20
Juliana Frossard Ribeiro Mendes, Egle Machado de Almeida Siqueira, João Gabriel Marques de Brito E Silva, Sandra Fernandes Arruda
BACKGROUND: Considering that vitamin A deficiency modulates hepcidin expression and consequently affects iron metabolism, we evaluated the effect of vitamin A deficiency in the expression of genes involved in the hemojuvelin (HJV)-bone morphogenetic protein 6 (BMP6)-small mothers against decapentaplegic protein (SMAD) signaling pathway. METHODS: Male Wistar rats were treated: control AIN-93G diet (CT), vitamin A-deficient diet (VAD), iron-deficient diet (FeD), vitamin A- and iron-deficient diet (VAFeD), or 12 mg all-trans retinoic acid (atRA)/kg diet...
2016: Genes & Nutrition
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