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beauveria bassiana secreted protein

Se Jin Lee, Yi-Ting Yang, Sihyeon Kim, Mi Rong Lee, Jong Cheol Kim, So Eun Park, Muktadir S Hossain, Tae Young Shin, Yu-Shin Nai, Jae Su Kim
BACKGROUND: Entomopathogenic Beauveria bassiana has been used as a biocontrol agent for insect pests, but its effect at the molecular level on the hosts has not been studied in detail. Herein, we performed transcriptome analysis of bean bug, Riptortus pedestris (Hemiptera: Alydidae) in response to infection with a highly virulent strain of B. bassiana JEF-007 (Bb JEF-007). RESULTS: Based on RNA-seq data from R. pedestris infected with Bb JEF-007 compared with non-infected bean bugs, infection was assumed to strongly activate 1) the energy production pathway by expressing dehydrogenases, 2) metabolic pathways by expressing secreted proteins, GTPase, MBF2 transcription factor family, pigment-dispersing factor, antioxidants, and cuticle proteins, and 3) the immune response pathway by expressing serine-threonine kinase in Toll pathway of bean bug...
June 11, 2018: Pest Management Science
Sen-Miao Tong, An-Xue Zhang, Chong-Tao Guo, Sheng-Hua Ying, Ming-Guang Feng
The fungal insect pathogen Beauveria bassiana has the blue-light photoreceptor VIVID (VVD) but lacks a pigmentation pattern to trace its light responses. Here, we show that the fungal vvd is transcriptionally expressed, and linked to other blue/red photoreceptor genes, in a daylight length-dependent manner. GFP-tagged VVD fusion protein was localized to periphery, cytoplasm and vacuoles of hyphal cells in light/dark (L:D) cycles of 24:0 and 16:8 and aggregated in cytoplasm with shortening daylight until transfer into nuclei in full darkness...
January 2018: Environmental Microbiology
Yiling Lai, Huan Chen, Ge Wei, Guandong Wang, Fang Li, Sibao Wang
The use of entomopathogenic fungi to control mosquitoes is a promising tool for reducing vector-borne disease transmission. To better understand infection stratagems of insect pathogenic fungi, we analyzed the global gene expression profiling of Beauveria bassiana at 36, 60, 84 and 108 h after topical infection of Anopheles stephensi adult mosquitoes using RNA sequencing (RNA-Seq). A total of 5,354 differentially expressed genes (DEGs) are identified over the course of fungal infection. When the fungus grows on the mosquito cuticle, up-regulated DEGs include adhesion-related genes involved in cuticle attachment, Pth11-like GPCRs hypothesized to be involved in host recognition, and extracellular enzymes involved in the degradation and penetration of the mosquito cuticle...
August 2017: Science China. Life Sciences
Zhen-Jian Chu, Huan-Huan Sun, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
High proportions of hypothetical proteins exist in genomic databases of fungi, including putative secretory proteins (PSPs) likely involved in fungal invasion and virulence. Here we characterize one of many PSPs revealed in the previous transcriptome of Beauveria bassiana (a fungal insect pathogen) infecting a global lepidopteran pest and name it vacuole-localized protein 4 (VLP4) because this small, domain-lacking protein (22.96 kDa) was specifically localized in the vacuoles of hyphal cells. Deletion of VLP4 resulted in repression of almost all genes acting in autophagy and central development pathways...
July 2017: Environmental Microbiology
Lidiia Vertyporokh, Iwona Wojda
The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between the insect hosts and their natural pathogens. In this report, we analyze the expression of a gene encoding an inducible metalloproteinase inhibitor (IMPI) in fat bodies of the greater wax moth larvae Galleria mellonella infected with an entomopathogenic fungus Beauveria bassiana...
2017: Acta Biochimica Polonica
Long-Bin Zhang, Li Tang, Sheng-Hua Ying, Ming-Guang Feng
Pil1A and Pil1B are two core eisosome proteins that are homologous to yeast Pil1/Lsp1 or filamentous fungal Pil1A/Pil1B but have been unexplored in entomopathogenic fungi. Here we examined subcellular localization and functions of Pil1A and Pil1B in Beauveria bassiana, a fungal insect pathogen. Either localization or co-localization experiments of the two proteins demonstrated that Pil1A and Pil1B were simultaneously localized at the periphery of hyphal cells for formation of stable, punctuate spots in B. bassiana...
May 2017: Environmental Microbiology
Zhen-Jian Chu, Yu-Jun Wang, Sheng-Hua Ying, Xiao-Wei Wang, Ming-Guang Feng
Genome-wide insight into insect pest response to the infection of Beauveria bassiana (fungal insect pathogen) is critical for genetic improvement of fungal insecticides but has been poorly explored. We constructed three pairs of transcriptomes of Plutella xylostella larvae at 24, 36 and 48 hours post treatment of infection (hptI) and of control (hptC) for insight into the host-pathogen interaction at genomic level. There were 2143, 3200 and 2967 host genes differentially expressed at 24, 36 and 48 hptI/hptC respectively...
2016: PloS One
Luciana S Lobo, Christian Luz, Éverton K K Fernandes, M Patricia Juárez, Nicolás Pedrini
Entomopathogenic fungi secrete toxic secondary metabolites during the invasion of the insect hemocoel as part of the infection process. Although these compounds have been frequently mentioned as virulence factors, the roles of many of them remain poorly understood, including the question of whether they are expressed during the infection process. A major hurdle to this issue remains the low sensitivity of biochemical detection techniques (e.g., HPLC) within the complex samples that may contain trace quantities of fungal molecules inside the insect...
June 2015: Journal of Invertebrate Pathology
Youshan Li, Ping Zhao, Huawei Liu, Xiaomeng Guo, Huawei He, Rui Zhu, Zhonghuai Xiang, Qingyou Xia
Entomopathogenic fungi penetrate the insect cuticle using their abundant hydrolases. These hydrolases, which include cuticle-degrading proteases and chitinases, are important virulence factors. Our recent findings suggest that many serine protease inhibitors, especially TIL-type protease inhibitors, are involved in insect resistance to pathogenic microorganisms. To clarify the molecular mechanism underlying this resistance to entomopathogenic fungi and identify novel genes to improve the silkworm antifungal capacity, we conducted an in-depth study of serine protease inhibitors...
February 2015: Insect Biochemistry and Molecular Biology
Jie Zhang, Anrui Lu, Lulu Kong, Qiaoli Zhang, Erjun Ling
Molting fluid accumulates between the old and new cuticles during periodical ecdysis in Ecdysozoa. Natural defects in insect ecdysis are frequently associated with melanization (an immunity response) occurring primarily in molting fluids, suggesting that molting fluid may impact immunity as well as affect ecdysis. To address this hypothesis, proteomic analysis of molting fluids from Bombyx mori during three different types of ecdysis was performed. Many proteins were newly identified, including immunity-related proteins, in each molting fluid...
December 26, 2014: Journal of Biological Chemistry
Yuqi Qin, Almudena Ortiz-Urquiza, Nemat O Keyhani
The filamentous fungus, Beauveria bassiana, is a ubiquitously distributed insect pathogen, currently used as an alternative to chemical pesticides for pest control. Conidiospores are the means by which the fungus disseminates in the environment, and these cells also represent the infectious agent most commonly used in field applications. Little, however, is known concerning the molecular basis for maintenance of spore viability, a critical feature for survival and persistence. Here, we report on the role of a putative methyltransferase, BbmtrA, in conidial viability, normal fungal growth and development, and virulence, via characterization of a targeted gene knockout strain...
November 2014: Microbiology
Xiu-Xiu Wang, Pu-Hong He, Ming-Guang Feng, Sheng-Hua Ying
SNF1/AMPK protein kinase plays important roles in fungal development and activation of catabolite-repressed genes. In this study, we characterized the role of the Beauveria bassiana SNF1 ortholog. The vegetative growth of the ΔBbSNF1 mutant was reduced by 16 to 50 % on diverse carbon/nitrogen sources. Transcriptional analysis revealed a collection of proteases and chitinases that were not induced when the mutant was grown on complex carbon/nitrogen sources. BbSNF1 also contributes to extracellular acidification...
October 2014: Applied Microbiology and Biotechnology
Zhibing Luo, Yuqi Qin, Yan Pei, Nemat O Keyhani
For most organisms, carbon and nitrogen uptake are essential for growth, development and, where applicable, pathogenesis. The role of the carbon catabolite repressor transcription factor homologue BbcreA in the entomopathogenic fungus Beauveria bassiana was investigated. Deletion of BbcreA resulted in pleiotropic effects, including nutrient toxicity, leading to a novel cell lytic phenotype. Fungal growth in rich media and minimal media containing select amino acids/peptides was severely compromised, with microscopic examination revealing conidial-base germ tube degeneration and cell lysis occurring during growth, a phenomenon exacerbated at higher temperatures (32°C)...
April 2014: Environmental Microbiology
B A Dias, P M O J Neves, L Furlaneto-Maia, M C Furlaneto
A Brazilian isolate of Beauveria bassiana (CG425) that shows high virulence against the coffee berry borer (CBB) was examined for the production of subtilisin-like (Pr1) and trypsin-like (Pr2) cuticle-degrading proteases. Fungal growth was either in nitrate-medium or in CBB cuticle-containing medium under both buffered and unbuffered conditions. In unbuffered medium supplemented with cuticle, the pH of cultures dropped and Pr1 and Pr2 activities were detected in high amounts only at a pH of 5.5 or higher. In buffered cultures, Pr1 and Pr2 activities were higher in medium supplemented with cuticle compared to activities with nitrate-medium...
April 2008: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Jun Xia, Chang-Rong Zhang, Shan Zhang, Fang-Fang Li, Ming-Guang Feng, Xiao-Wei Wang, Shu-Sheng Liu
BACKGROUND: The fungal pathogen, Beauveria bassiana, is an efficient biocontrol agent against a variety of agricultural pests. A thorough understanding of the basic principles of insect-fungus interactions may enable the genetic modification of Beauveria bassiana to enhance its virulence. However, the molecular mechanism of insect response to Beauveria bassiana infection is poorly understood, let alone the identification of fungal virulent factors involved in pathogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Here, next generation sequencing technology was applied to examine the expression of whitefly (Bemisia tabaci) genes in response to the infection of Beauveria bassiana...
2013: PloS One
Yanhua Fan, Almudena Ortiz-Urquiza, Ramsha A Kudia, Nemat O Keyhani
Neuronal calcium sensor proteins and their homologues participate in transducing extracellular signals that affect intracellular Ca(2+) levels, which in turn regulate enzyme activities, secretion, gene expression and other biological processes. The filamentous fungus Beauveria bassiana is a broad-host-range pathogen of insects that acidifies the extracellular milieu during growth and pathogenesis towards target hosts. A collection of B. bassiana random insertion mutants were screened on pH indicator plates and one mutant was isolated that displayed reduced acidification...
July 2012: Microbiology
A Ortiz-Urquiza, L Riveiro-Miranda, C Santiago-Álvarez, E Quesada-Moraga
Fungal virulence has been mostly associated with cuticle-degrading enzymes that can be regulated depending on nutrient conditions. However, few studies have related fungal virulence to insect-toxic secreted proteins. Here, we describe how the presence of secreted toxic proteins may be linked to conidial virulence, which can be affected by nutrient factors. In this study we evaluated: (1) the virulence of the conidia of four Beauveria bassiana strains (EABb 01/103-Su, EABb 01/12-Su, EABb 01/88-Su and EABb 01/110-Su) grown on three different media (malt extract agar (MA), Rice (Rice), Sabouraud dextrose agar (SDA) and harvested from the cadavers of fungal-infected Galleria mellonella larvae (CAD) and (2) the toxicity of the crude soluble protein extracts (CSPEs) obtained from Adamek's liquid medium inoculated with these conidia...
November 2010: Journal of Invertebrate Pathology
Hong You, Hu Wan, Jianhong Li, Byung Rae Jin
Peptidoglycan recognition proteins (PGRPs) are pattern recognition molecules of the innate immune system that recognize peptidoglycan, a unique bacterial cell wall component. Here we cloned and characterized PGRP-S from the bumblebee Bombus ignitus (BiPGRP-S). The BiPGRP-S gene consists of four exons that encode 194 amino acid residues. Comparative analysis indicates that the predicted amino acid sequence of BiPGRP-S shares a high identity with enzymatically active PGRP-S proteins and contains the amino acids required for amidase activity...
September 2010: Developmental and Comparative Immunology
Weiguo Fang, Jin Feng, Yanhua Fan, Yongjun Zhang, Michael J Bidochka, Raymond J St Leger, Yan Pei
Entomopathogenic fungi, such as Beauveria bassiana and Metarhizium anisopliae are being developed as alternatives to chemical insecticides. They infect insects by direct penetration of the cuticle using a combination of physical pressure and extracellular hydrolytic enzymes such as proteases and chitinases. Previously we found that overexpression of a subtilisin-like protease (Pr1A) or a chitinase (Bbchit1) resulted in increased virulence of M. anisopliae and B. bassiana, respectively. In this study, we found that a mixture of the B...
October 2009: Journal of Invertebrate Pathology
Sohail S Qazi, George G Khachatourians
Secretion of catabolic extracellular enzymes (ECE) is the hallmark of the infection of insects through the cuticle by entomopathogenic fungi (EPF). In this paper, we show that germinating conidia of Beauveria bassiana (Bb) regulate the synthesis of ECE through a multiple control mode during the initial stages of germination. We tested Bb conidial growth on aphid exuviae with or without supplementation of additional carbon and/or nitrogen (C/N) compounds. To understand the interrelation between conidial germination during growth, the synthesis of ECE activity, free amino nitrogen (FAN), glucose and fungal dry weight biomass were measured...
June 2008: Archives of Microbiology
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