keyword
MENU ▼
Read by QxMD icon Read
search

fungal secreted protein

keyword
https://www.readbyqxmd.com/read/29158777/combined-genome-and-transcriptome-sequencing-to-investigate-the-plant-cell-wall-degrading-enzyme-system-in-the-thermophilic-fungus-malbranchea-cinnamomea
#1
Silvia Hüttner, Thanh Thuy Nguyen, Zoraide Granchi, Thomas Chin-A-Woeng, Dag Ahrén, Johan Larsbrink, Vu Nguyen Thanh, Lisbeth Olsson
Background: Genome and transcriptome sequencing has greatly facilitated the understanding of biomass-degrading mechanisms in a number of fungal species. The information obtained enables the investigation and discovery of genes encoding proteins involved in plant cell wall degradation, which are crucial for saccharification of lignocellulosic biomass in second-generation biorefinery applications. The thermophilic fungus Malbranchea cinnamomea is an efficient producer of many industrially relevant enzymes and a detailed analysis of its genomic content will considerably enhance our understanding of its lignocellulolytic system and promote the discovery of novel proteins...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29146248/phosphatidylcholine-absence-affects-the-secretion-of-lipodepsipeptide-phytoxins-in-pseudomonas-syringae-pv-syringae-van-hall-cfcc-1336
#2
Fang Cao, Min Xiong, Shunyi Li, Huawan Cai, Yufang Sun, Sheng Yang, Xin Liu, Rong Zhu, Xuejing Yu, Xingguo Wang
Pseudomonas syringae pv. syringae van Hall CFCC 1336 (Pss 1336) is the causal agent of bacterial disease of stone fruit trees, and also able to elicit hypersensitive response (HR) in non-host tobacco. It is known that this pathogen uses PCS-pathway to synthesize phosphatidylcholine (PC), and mutation of the pcs gene abolishes bacterial PC synthesis. Previous study also found that the 1336 pcs(-) mutant lacking PC in its membrane phospholipids was unable to secrete HrpZ harpin and elicit HR in non-host plants...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29131484/the-major-cellulases-cbh-1-and-cbh-2-of-neurospora-crassa-rely-on-distinct-er-cargo-adaptors-for-efficient-er-exit
#3
Trevor Starr, A Pedro Gonçalves, Neeka Meshgin, N Louise Glass
Filamentous fungi are native secretors of lignocellulolytic enzymes and are used as protein-producing factories in the industrial biotechnology sector. Despite the importance of these organisms in industry, relatively little is known about the filamentous fungal secretory pathway or how it might be manipulated for improved protein production. Here we use Neurospora crassa as a model filamentous fungus to interrogate the requirements for trafficking of cellulase enzymes from the endoplasmic reticulum to the Golgi...
November 13, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29119000/comparing-the-physiochemical-parameters-of-three-celluloses-reveals-new-insights-into-substrate-suitability-for-fungal-enzyme-production
#4
Lara Hassan, Manfred J Reppke, Nils Thieme, Steffen A Schweizer, Carsten W Mueller, J Philipp Benz
Background: The industrial applications of cellulases are mostly limited by the costs associated with their production. Optimized production pathways are therefore desirable. Based on their enzyme inducing capacity, celluloses are commonly used in fermentation media. However, the influence of their physiochemical characteristics on the production process is not well understood. In this study, we examined how physical, structural and chemical properties of celluloses influence cellulase and hemicellulase production in an industrially-optimized and a non-engineered filamentous fungus: Trichoderma reesei RUT-C30 and Neurospora crassa...
2017: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29109463/a-role-for-small-secreted-proteins-ssps-in-a-saprophytic-fungal-lifestyle-ligninolytic-enzyme-regulation-in-pleurotus-ostreatus
#5
Daria Feldman, David J Kowbel, N Louise Glass, Oded Yarden, Yitzhak Hadar
Small secreted proteins (SSPs), along with lignocellulose degrading enzymes, are integral components of the secretome of Pleurotus ostreatus, a white rot fungus. In this study, we identified 3 genes (ssp1, 2 and 3) encoding proteins that are annotated as SSPs and that exhibited of ~4,500- fold expression, 24 hr following exposure to the toxic compound 5-hydroxymethylfurfural (HMF). Homologues to genes encoding these SSPs are present in the genomes of other basidiomycete fungi, however the role of SSPs is not yet understood...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29109176/candidalysin-drives-epithelial-signaling-neutrophil-recruitment-and-immunopathology-at-the-vaginal-mucosa
#6
Jonathan P Richardson, Hubertine M E Willems, David L Moyes, Saeed Shoaie, Katherine S Barker, Shir Lynn Tan, Glen E Palmer, Bernhard Hube, Julian R Naglik, Brian M Peters
Unlike other forms of candidiasis, vulvovaginal candidiasis, caused primarily by the fungal pathogen Candida albicans, is a disease of immunocompetent and otherwise healthy women. Despite its prevalence, the fungal factors responsible for initiating symptomatic infection remain poorly understood. One of the hallmarks of vaginal candidiasis is the robust recruitment of neutrophils to the site of infection, which seemingly do not clear the fungus, but rather exacerbate disease symptomatology. Candidalysin, a newly discovered peptide toxin secreted by C...
November 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29105533/plant-immunity-and-symbiosis-signaling-mediated-by-lysm-receptors
#7
Yoshitake Desaki, Kana Miyata, Maruya Suzuki, Naoto Shibuya, Hanae Kaku
Plants possess the ability to recognize microbe-associated molecular patterns (MAMPs) and PAMPs through the PRRs, and initiate pattern-triggered immunity. MAMPs are derived from cell-envelope components, secreted materials and cytosolic proteins from bacteria, oomycetes or fungi, and some MAMPs play a similar function in the innate immunity in mammals. Chitin is a representative fungal MAMP and triggers defense signaling in a wide range of plant species. The chitin receptors CEBiP and CERK1 on the plasma membrane have LysM (lysin motif) in their ectodomains...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29105094/expression-of-tomato-prosystemin-gene-in-arabidopsis-reveals-systemic-translocation-of-its-mrna-and-confers-necrotrophic-fungal-resistance
#8
Haiyan Zhang, Pengli Yu, Jiuhai Zhao, Hongling Jiang, Haiyang Wang, Yingfang Zhu, Miguel A Botella, Jozef Šamaj, Chuanyou Li, Jinxing Lin
Systemin (SYS), an octadecapeptide hormone processed from a 200-amino-acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whether PS mRNA is transferable and its role in systemic defense responses remain unknown. We created the transgenic tomato PS gene tagged with the green fluorescent protein (PS-GFP) using a shoot- or root-specific promoter, and the constitutive 35S promoter in Arabidopsis. Subcellular localization of PS-/SYS-GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real-time PCR...
November 3, 2017: New Phytologist
https://www.readbyqxmd.com/read/29083038/plasmodesmal-regulation-during-plant-pathogen-interactions
#9
REVIEW
Cecilia Cheval, Christine Faulkner
Contents I. II. III. IV. V. VI. VII. References SUMMARY: Plasmodesmata (PD) are plasma membrane-lined pores that connect neighbouring plant cells, bridging the cell wall and establishing cytoplasmic and membrane continuity between cells. PD are dynamic structures regulated by callose deposition in a variety of stress and developmental contexts. This process crudely controls the aperture of the pore and thus the flux of molecules between cells. During pathogen infection, plant cells initiate a range of immune responses and it was recently identified that, following perception of fungal and bacterial pathogens, plant cells initially close their PD...
October 30, 2017: New Phytologist
https://www.readbyqxmd.com/read/29068240/a-verticillium-dahliae-extracellular-cutinase-modulates-plant-immune-responses
#10
Yuejing Gui, Wenqi Zhang, Dandan Zhang, Lei Zhou, Dylan P G Short, Jie Wang, Xuefeng Ma, Tinggang Li, Zhiqiang Kong, Bao-Li Wang, Dan Wang, Nanyang Li, Krishna Subbarao, Jie-Yin Chen, Xiaofeng Dai
Cutinases have been implicated as important enzymes during the process of fungal infection of aerial plant organs. The function of cutinases in the disease cycle of fungal pathogens that invade plants through the roots has been less studied. Here, functional analysis of thirteen cutinase (carbohydrate esterase family 5 domain-containing) genes (VdCUTs) in the highly virulent vascular wilt pathogen Verticillium dahliae Vd991 was performed. Significant sequence divergence in cutinase family members was observed in the genome of V...
October 25, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29062087/predicted-microbial-secretomes-and-their-target-substrates-in-marine-sediment
#11
William D Orsi, Thomas A Richards, Warren R Francis
Scientific drilling has identified a biosphere in marine sediments (1) , which contain many uncultivated microbial groups known only by their DNA sequences (2-4) . Recycling of organic matter in sediments is an important component of biogeochemical cycles because marine sediments are critical for long-term carbon storage (5) . Turnover of carbon is hypothesized to be driven by the secretion of enzymes by microbial organisms (5-7) , which act to break down macromolecules into constitutive monomers that can be transported into cells...
October 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/29043572/secreted-proteins-produced-by-fungi-associated-with-botryosphaeria-dieback-trigger-distinct-defense-responses-in-vitis-vinifera-and-vitis-rupestris-cells
#12
E Stempien, M-L Goddard, Y Leva, M Bénard-Gellon, H Laloue, S Farine, F Kieffer-Mazet, C Tarnus, C Bertsch, J Chong
Grapevine trunk diseases (Eutypa dieback, esca and Botryosphaeria dieback) are caused by a complex of xylem-inhabiting fungi, which severely reduce yields in vineyards. Botryosphaeria dieback is associated with Botryosphaeriaceae. In order to develop effective strategies against Botryosphaeria dieback, we investigated the molecular basis of grapevine interactions with a virulent species, Neofusicoccum parvum, and a weak pathogen, Diplodia seriata. We investigated defenses induced by purified secreted fungal proteins within suspension cells of Vitis (Vitis rupestris and Vitis vinifera cv...
October 17, 2017: Protoplasma
https://www.readbyqxmd.com/read/29042440/plant-pathogenesis-related-proteins-of-the-cacao-fungal-pathogen-moniliophthora-perniciosa-differ-in-their-lipid-binding-specificities
#13
Rabih Darwiche, Ola El Atab, Renata Moro Baroni, Paulo J P L Teixeira, Jorge M C Mondego, Gonçalo A G Pereira, Roger Schneiter
Moniliophthora perniciosa is the causative agent of witches' broom disease, which devastates cacao cultures in South America. This pathogenic fungus infects meristematic tissues and derives nutrients from the plant apoplast during an unusually long-lasting biotrophic stage. In order to survive, the fungus produces proteins to suppress the plant immune response. Proteins of the Pathogenesis Related 1 (PR-1)/CAP superfamily have been implicated in fungal virulence and immune suppression. The genome of M. perniciosa encodes eleven homologues of plant PR-1 proteins, designated MpPR-1 proteins, but their precise mode of action is poorly understood...
October 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29038760/cellobiohydrolase-b-of-aspergillus-niger-over-expressed-in-pichia-pastoris-stimulates-hydrolysis-of-oil-palm-empty-fruit-bunches
#14
James Sy-Keen Woon, Mukram M Mackeen, Rosli M Illias, Nor M Mahadi, William J Broughton, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
BACKGROUND: Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-glucosidases, endoglucanases and cellobiohydrolases. Cellobiohydrolases (CBH) are vital to the degradation of crystalline cellulose present in lignocellulosic biomass. However, A. niger naturally secretes low levels of CBH. Hence, recombinant production of A...
2017: PeerJ
https://www.readbyqxmd.com/read/29028000/influence-of-resistance-breeding-in-common-bean-on-rhizosphere-microbiome-composition-and-function
#15
Lucas William Mendes, Jos M Raaijmakers, Mattias de Hollander, Rodrigo Mendes, Siu Mui Tsai
The rhizosphere microbiome has a key role in plant growth and health, providing a first line of defense against root infections by soil-borne pathogens. Here, we investigated the composition and metabolic potential of the rhizobacterial community of different common bean (Phaseolus vulgaris) cultivars with variable levels of resistance to the fungal root pathogen Fusarium oxysporum (Fox). For the different bean cultivars grown in two soils with contrasting physicochemical properties and microbial diversity, rhizobacterial abundance was positively correlated with Fox resistance...
October 13, 2017: ISME Journal
https://www.readbyqxmd.com/read/29020037/a-conserved-fungal-glycosyltransferase-facilitates-pathogenesis-of-plants-by-enabling-hyphal-growth-on-solid-surfaces
#16
Robert King, Martin Urban, Rebecca P Lauder, Nichola Hawkins, Matthew Evans, Amy Plummer, Kirstie Halsey, Alison Lovegrove, Kim Hammond-Kosack, Jason J Rudd
Pathogenic fungi must extend filamentous hyphae across solid surfaces to cause diseases of plants. However, the full inventory of genes which support this is incomplete and many may be currently concealed due to their essentiality for the hyphal growth form. During a random T-DNA mutagenesis screen performed on the pleomorphic wheat (Triticum aestivum) pathogen Zymoseptoria tritici, we acquired a mutant unable to extend hyphae specifically when on solid surfaces. In contrast "yeast-like" growth, and all other growth forms, were unaffected...
October 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29016942/ben3-big2-arf-gef-is-involved-in-brefeldin-a-sensitive-trafficking-at-the-trans-golgi-network-early-endosome-in-arabidopsis-thaliana
#17
Saeko Kitakura, Maciek Adamowski, Yuki Matsuura, Luca Santuari, Hirotaka Kouno, Kohei Arima, Christian S Hardtke, Jirí Friml, Tatsuo Kakimoto, Hirokazu Tanaka
Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA), which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM...
August 21, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28986379/functional-analysis-of-the-glucan-degradation-locus-gdl-in-caldicellulosiruptor-bescii-reveals-essential-roles-of-component-glycoside-hydrolases-in-plant-biomass-deconstruction
#18
Jonathan M Conway, Bennett S McKinley, Nathaniel L Seals, Diana Hernandez, Piyum A Khatibi, Suresh Poudel, Richard J Giannone, Robert L Hettich, Amanda M Williams-Rhaesa, Gina L Lipscomb, Michael W W Adams, Robert M Kelly
The ability to hydrolyze microcrystalline cellulose is an uncommon feature in the microbial world, but one that can be exploited for conversion of lignocellulosic feedstocks into bio-based fuels and chemicals. Understanding the physiological and biochemical mechanisms by which microorganisms deconstruct cellulosic material is key to achieving this objective. The Glucan Degradation Locus (GDL) in the genomes of extremely thermophilic Caldicellulosiruptor species encodes polysaccharide lyases (PLs), unique cellulose binding proteins (tāpirins), and putative post-translational modifying enzymes, in addition to multi-domain, multi-functional glycoside hydrolases (GHs), thereby representing an alternative paradigm for plant biomass degradation, as compared to fungal or cellulosomal systems...
October 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28983472/aspartic-proteases-and-major-cell-wall-components-in-candida-albicans-trigger-the-release-of-neutrophil-extracellular-traps
#19
Marcin Zawrotniak, Oliwia Bochenska, Justyna Karkowska-Kuleta, Karolina Seweryn-Ozog, Wataru Aoki, Mitsuyoshi Ueda, Andrzej Kozik, Maria Rapala-Kozik
Neutrophils use different mechanisms to cope with pathogens that invade the host organism. The most intriguing of these responses is a release of neutrophil extracellular traps (NETs) composed of decondensed chromatin and granular proteins with antimicrobial activity. An important potential target of NETs is Candida albicans-an opportunistic fungal pathogen that employs morphological and phenotype switches and biofilm formation during contact with neutrophils, accompanied by changes in epitope exposition that mask the pathogen from host recognition...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28970504/ant-infecting-ophiocordyceps-genomes-reveal-a-high-diversity-of-potential-behavioral-manipulation-genes-and-a-possible-major-role-for-enterotoxins
#20
Charissa de Bekker, Robin A Ohm, Harry C Evans, Andreas Brachmann, David P Hughes
Much can be gained from revealing the mechanisms fungal entomopathogens employ. Especially intriguing are fungal parasites that manipulate insect behavior because, presumably, they secrete a wealth of bioactive compounds. To gain more insight into their strategies, we compared the genomes of five ant-infecting Ophiocordyceps species from three species complexes. These species were collected across three continents, from five different ant species in which they induce different levels of manipulation. A considerable number of (small) secreted and pathogenicity-related proteins were only found in these ant-manipulating Ophiocordyceps species, and not in other ascomycetes...
October 2, 2017: Scientific Reports
keyword
keyword
97465
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"