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fungal secreted protein

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https://www.readbyqxmd.com/read/28633330/the-asparagine-rich-protein-nrp-interacts-with-the-verticillium-effector-pevd1-and-regulates-the-subcellular-localization-of-cryptochrome-2
#1
Ruimin Zhou, Tong Zhu, Lei Han, Mengjie Liu, Mengyuan Xu, Yanli Liu, Dandan Han, Dewen Qiu, Qingqiu Gong, Xinqi Liu
The soil-borne fungal pathogen Verticillium dahliae infects a wide range of dicotyledonous plants including cotton, tobacco, and Arabidopsis. Among the effector proteins secreted by V. dahliae, the 16 kDa PevD1 induces a hypersensitive response in tobacco. Here we report the high-resolution structure of PevD1 with folds resembling a C2 domain-like structure with a calcium ion bound to the C-terminal acidic pocket. A yeast two-hybrid screen, designed to probe for molecular functions of PevD1, identified Arabidopsis asparagine-rich protein (NRP) as the interacting partner of PevD1...
June 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28610916/the-rhizoferrin-biosynthetic-gene-in-the-fungal-pathogen-rhizopus-delemar-is-a-novel-member-of-the-nis-gene-family
#2
Cassandra S Carroll, Clark L Grieve, Indu Murugathasan, Andrew J Bennet, Clarissa M Czekster, Huanting Lui, James Naismith, Margo M Moore
Iron is essential for growth and in low iron environments such as serum many bacteria and fungi secrete ferric iron-chelating molecules called siderophores. All fungi produce hydroxamate siderophores with the exception of Mucorales fungi, which secrete rhizoferrin, a polycarboxylate siderophore. Here we investigated the biosynthesis of rhizoferrin by the opportunistic human pathogen, Rhizopus delemar. We searched the genome of R. delemar 99-880 for a homologue of the bacterial NRPS-independent siderophore (NIS) protein, SfnaD that is involved in biosynthesis of staphyloferrin A in Staphylococcus aureus...
June 10, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28602730/recombinant-expression-of-a-laccase-from-coriolopsis-gallica-in-pichia-pastoris-using-a-modified-%C3%AE-factor-preproleader
#3
Mayra Avelar, Clarita Olvera, Denise Aceves-Zamudio, Jorge Luis Folch, Marcela Ayala
In this work we communicate the heterologous expression of a laccase from Coriolopsis gallica in Pichia pastoris. This enzyme has been reported to efficiently degrade a variety of pollutants such as industrial dyes. The expression strategy included using a previously reported modified α-factor preproleader for enhanced secretion and pAOX1, a methanol-responsive promoter. Methanol concentration, copper salts concentration and temperature were varied in order to enhance laccase expression in this heterologous system...
June 5, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28599020/isg15-in-host-defense-against-candida-albicans-infection-in-a-mouse-model-of-fungal-keratitis
#4
Chen Dong, Nan Gao, Bing X Ross, Fu-Shin X Yu
Purpose: ISG15, a di-ubiquitin-like protein, is critical for controlling certain viral and bacterial infections. We sought to determine if ISG15 plays a role in corneal innate immunity against Candida albicans (C. albicans) using a C57BL/6 (B6) mouse model of human fungal keratitis. Methods: Scarified corneas of adult B6 mice were pretreated with TLR5 ligand flagellin and then inoculated with C. albicans. The expression of ISG15 and other genes involved in ISG15 conjugation (ISGylation) was determined by real-time PCR...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28592950/purification-and-characterization-of-a-major-extracellular-chitinase-from-a-biocontrol-bacterium-paenibacillus-elgii-hoa73
#5
Yong Hwan Kim, Seur Kee Park, Jin Young Hur, Young Cheol Kim
Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type III domains, and a catalytic hydrolase domain...
June 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28588252/candida-albicans-sap6-amyloid-regions-function-in-cellular-aggregation-and-zinc-binding-and-contribute-to-zinc-acquisition
#6
Rohitashw Kumar, Christine Breindel, Darpan Saraswat, Paul J Cullen, Mira Edgerton
Candida albicans is an opportunistic fungal pathogen colonizing the oral cavity. C. albicans secreted aspartic protease Sap6 is important for virulence during oral candidiasis since it degrades host tissues to release nutrients and essential transition metals. We found that zinc specifically increased C. albicans autoaggregation induced by Sap6; and that Sap6 itself bound zinc ions. In silico analysis of Sap6 predicted four amyloidogenic regions that were synthesized as peptides (P1-P4). All peptides, as well as full length Sap6, demonstrated amyloid properties, and addition of zinc further increased amyloid formation...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578805/expression-system-for-heterologous-protein-expression-in-the-filamentous-fungus-aspergillus-unguis
#7
Aravind Madhavan, Ashok Pandey, Rajeev K Sukumaran
Heterologous protein expression in filamentous fungi is advantageous, especially in the context of large scale production of high volume low value recombinant proteins. However, such systems are rare and not available in public domain. A novel filamentous fungus - Aspergillus unguis NII 08123 was used as host for developing a protein expression system. An expression cassette was assembled using A. nidulans glyceraldehyde 3 phosphate dehydrogenase promoter (Pgapd), tryptophan synthase transcription terminator (TtrpC) and hygromycin resistance gene (hph) as selection marker...
May 22, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28575401/localization-of-arabidopsis-forked1-to-a-raba-positive-compartment-suggests-a-role-in-secretion
#8
Neema Prabhakaran Mariyamma, Hongwei Hou, Francine M Carland, Timothy Nelson, Elizabeth A Schultz
When FORKED1 (FKD1) is mutated, asymmetric localization of PINFORMED1 (PIN1), particularly to the apical side of cells, fails to occur properly in developing veins, resulting in an open vein pattern. FKD1 encodes a protein with a Pleckstrin homology-like (PL) domain, suggesting interaction with phosphoinositides. FKD1 has been previously found to interact with an ADP ribosylation factor GTPase-activating protein (ARF-GAP) important for vein patterning, SCARFACE/VAN3 (SFC). We find that FKD1-green fluorescent protein (GFP) localizes to the plasma membrane and to punctae labeled by SFC-yellow fluorescent protein (YFP)...
June 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28557308/discovery-of-a-new-intravacuolar-protein-required-for-the-autophagy-development-and-virulence-of-beauveria-bassiana
#9
Zhen-Jian Chu, Huan-Huan Sun, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
High proportions of hypothetical proteins exist in genomic databases of fungi, including putative secretory proteins (PSPs) likely involved in fungal invasion and virulence. Here we characterize one of many PSPs revealed in the previous transcriptome of Beauveria bassiana (a fungal insect pathogen) infecting a global lepidopteran pest and name it vacuole-localized protein 4 (VLP4) because this small, domain-lacking protein (22.96 kDa) was specifically localized in the vacuoles of hyphal cells. Deletion of VLP4 resulted in repression of almost all genes acting in autophagy and central development pathways...
May 30, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28520093/verticillium-dahliae-transcription-factor-vdftf1-regulates-the-expression-of-multiple-secreted-virulence-factors-and-is-required-for-full-virulence-in-cotton
#10
Wen-Qi Zhang, Yue-Jing Gui, Dylan P G Short, Ting-Gang Li, Dan-Dan Zhang, Lei Zhou, Chun Liu, Yu-Ming Bao, Krishna V Subbarao, Jie-Yin Chen, Xiao-Feng Dai
Fungal transcription factors (TFs) implicated in the regulation of virulence gene expression have been identified in a number of plant pathogens. In Verticillium dahliae, despite its agricultural importance, few regulators of transcription have been characterized. In this study, a T-DNA insertional mutant with significantly reduced virulence toward cotton was identified. The T-DNA was traced to VdFTF1, a gene encoding a TF containing a Fungal_trans domain. Transient expression in onion epidermal cells indicated that VdFTF1 is localized to the nucleus...
May 18, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28512683/messenger-rna-transport-in-the-opportunistic-fungal-pathogen-candida-albicans
#11
REVIEW
Anne E McBride
Candida albicans, a common commensal fungus, can cause disease in immunocompromised hosts ranging from mild mucosal infections to severe bloodstream infections with high mortality rates. The ability of C. albicans cells to switch between a budding yeast form and an elongated hyphal form is linked to pathogenicity in animal models. Hyphal-specific proteins such as cell-surface adhesins and secreted hydrolases facilitate tissue invasion and host cell damage, but the specific mechanisms leading to asymmetric protein localization in hyphae remain poorly understood...
May 16, 2017: Current Genetics
https://www.readbyqxmd.com/read/28511724/production-of-a-recombinant-swollenin-from-trichoderma-harzianum-in-escherichia-coli-and-its-potential-synergistic-role-in-biomass-degradation
#12
Clelton A Santos, Jaire A Ferreira-Filho, Anthonia O'Donovan, Vijai K Gupta, Maria G Tuohy, Anete P Souza
BACKGROUND: Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of cellulose structure by loosening macrofibrils. Consequently, SWOs can increase the accessibility and efficiency of the other enzymes involved in the saccharification of cellulosic substrates. Thus, SWOs are promising targets for improving the hydrolysis of plant biomass and for use as an additive to enhance the efficiency of an enzyme cocktail designed for the production of biofuels...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28504466/nepetoidin-b-a-natural-product-inhibits-lps-stimulated-nitric-oxide-production-via-modulation-of-inos-mediated-by-nf-%C3%AE%C2%BAb-mkp-5-pathways
#13
Xiaxia Wu, Hongwei Gao, Wen Sun, Jie Yu, Hao Hu, Qiongming Xu, Xiuping Chen
Previous reports showed that nepetoidin B (NTB), a natural product isolated from many herbs, has anti-fungal and anti-bacterial effects. In this study, the antiinflammatory effect of NTB was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The cytotoxic effect of NTB and LPS was determined by MTT assay. The nitric oxide (NO) production was detected by Griess assay. The TNF-α and IL-6 levels were determined by enzyme-linked immunosorbent assay kits. Protein expressions were tested by western blotting...
May 15, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28484941/proteomics-as-a-tool-to-identify-new-targets-against-aspergillus-and-scedosporium-in-the-context-of-cystic-fibrosis
#14
Andoni Ramirez-Garcia, Aize Pellon, Idoia Buldain, Aitziber Antoran, Aitana Arbizu-Delgado, Xabier Guruceaga, Aitor Rementeria, Fernando L Hernando
Cystic fibrosis (CF) is a genetic disorder that increases the risk of suffering microbial, including fungal, infections. In this paper, proteomics-based information was collated relating to secreted and cell wall proteins with potential medical applications from the most common filamentous fungi in CF, i.e., Aspergillus and Scedosporium/Lomentospora species. Among the Aspergillus fumigatus secreted allergens, β-1,3-endoglucanase, the alkaline protease 1 (Alp1/oryzin), Asp f 2, Asp f 13/15, chitinase, chitosanase, dipeptidyl-peptidase V (DppV), the metalloprotease Asp f 5, mitogillin/Asp f 1, and thioredoxin reductase receive a special mention...
May 8, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28479603/ustilago-maydis-effectors-and-their-impact-on-virulence
#15
REVIEW
Daniel Lanver, Marie Tollot, Gabriel Schweizer, Libera Lo Presti, Stefanie Reissmann, Lay-Sun Ma, Mariana Schuster, Shigeyuki Tanaka, Liang Liang, Nicole Ludwig, Regine Kahmann
Biotrophic fungal plant pathogens establish an intimate relationship with their host to support the infection process. Central to this strategy is the secretion of a range of protein effectors that enable the pathogen to evade plant immune defences and modulate host metabolism to meet its needs. In this Review, using the smut fungus Ustilago maydis as an example, we discuss new insights into the effector repertoire of smut fungi that have been gained from comparative genomics and discuss the molecular mechanisms by which U...
July 2017: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/28468279/applying-unconventional-secretion-in-ustilago-maydis-for-the-export-of-functional-nanobodies
#16
Marius Terfrüchte, Michèle Reindl, Silke Jankowski, Parveen Sarkari, Michael Feldbrügge, Kerstin Schipper
Exploiting secretory pathways for production of heterologous proteins is highly advantageous with respect to efficient downstream processing. In eukaryotic systems the vast majority of heterologous proteins for biotechnological application is exported via the canonical endoplasmic reticulum-Golgi pathway. In the endomembrane system target proteins are often glycosylated and may thus be modified with foreign glycan patterns. This can be destructive for their activity or cause immune reactions against therapeutic proteins...
April 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28464870/comparative-analysis-of-the-predicted-secretomes-of-rosaceae-scab-pathogens-venturia-inaequalis-and-v-pirina-reveals-expanded-effector-families-and-putative-determinants-of-host-range
#17
Cecilia H Deng, Kim M Plummer, Darcy A B Jones, Carl H Mesarich, Jason Shiller, Adam P Taranto, Andrew J Robinson, Patrick Kastner, Nathan E Hall, Matthew D Templeton, Joanna K Bowen
BACKGROUND: Fungal plant pathogens belonging to the genus Venturia cause damaging scab diseases of members of the Rosaceae. In terms of economic impact, the most important of these are V. inaequalis, which infects apple, and V. pirina, which is a pathogen of European pear. Given that Venturia fungi colonise the sub-cuticular space without penetrating plant cells, it is assumed that effectors that contribute to virulence and determination of host range will be secreted into this plant-pathogen interface...
May 2, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28464106/breeding-and-genetics-symposium-breeding-heat-tolerant-dairy-cattle-the-case-for-introgression-of-the-slick-prolactin-receptor-variant-into-dairy-breeds
#18
S R Davis, R J Spelman, M D Littlejohn
Increasing environmental temperatures are a threat to the sustainability of livestock production and, because of the high metabolic demands of lactation, to dairy production in particular. Summer heat waves in temperate climates reduce feed intake, milk production, and cow comfort. In extreme heat events, there is an increase in cow mortality. In tropical climates, dairy cattle are mostly (zebu) type or zebu crossbred with temperate dairy breeds. Crossbreeding is undertaken to combine the heat tolerance and tick resistance of zebu with the productivity of temperate dairy breeds...
April 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28460240/how-filamentous-plant-pathogen-effectors-are-translocated-to-host-cells
#19
REVIEW
Libera Lo Presti, Regine Kahmann
The interaction of microbes with "signature" plants is largely governed by secreted effector proteins, which serve to dampen plant defense responses and modulate host cell processes. Secreted effectors can function either in the apoplast or within plant cell compartments. How oomycetes and fungi translocate their effectors to plant cells is still poorly understood and controversial. While most oomycete effectors share a common 'signature' that was proposed to mediate their uptake via endocytosis, fungal effectors display no conserved motifs at the primary amino acid sequence level...
April 28, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28437139/protection-against-common-bean-rust-conferred-by-a-gene-silencing-method
#20
Bret Cooper, Kimberly B Campbell
Rust disease of the dry bean plant, Phaseolus vulgaris, is caused by the fungus Uromyces appendiculatus. The fungus acquires its nutrients and energy from bean leaves using a specialized cell structure, the haustorium, through which it secretes effector proteins that contribute to pathogenicity by defeating the plant immune system. Candidate effectors have been identified by DNA sequencing and motif analysis, and some candidates have been observed in infected leaves by mass spectrometry. To assess their roles in pathogenicity, we have inserted small fragments of genes for five candidates into bean pod mottle virus...
April 24, 2017: Phytopathology
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