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fungal secreted protein

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https://www.readbyqxmd.com/read/27876506/fungal-peptides-from-pneumonitis-hypersensitivity-etiologic-agents-are-able-to-induce-specific-cellular-immune-response
#1
Anne-Pauline Bellanger, Thibaud Lignon, Yann Godet, Bénédicte Rognon, Gabriel Reboux, Houssein Gbaguidi-Haore, Christophe Borg, Laurence Millon
PURPOSE: Hypersensitivity pneumonitis (HP) is an immunoallergic disease due to chronic exposure to high quantities of different microorganisms such as Mycobacterium immunogenum (Mi), a mycobacterium, and Lichtheimia corymbifera (Lc), a filamentous fungus. It has recently been demonstrated that the protein DLDH (dihydrolipoyl dehydrogenase), is common to these microorganisms. This study aimed to investigate the immune potential of overlapping peptide pools covering the MiDLDH and LcDLDH...
November 19, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27868319/infection-assays-in-arabidopsis-reveal-candidate-effectors-from-the-poplar-rust-fungus-that-promote-susceptibility-to-bacteria-and-oomycete-pathogens
#2
Hugo Germain, David L Joly, Caroline Mireault, Mélodie B Plourde, Claire Letanneur, Donald Stewart, Marie-Josée Morency, Benjamin Petre, Sébastien Duplessis, Armand Séguin
Fungi of the Pucciniales order cause rust diseases, which altogether affect thousands of plant species worldwide and pose major threat to several crops. How rust effectors - virulence proteins delivered into infected tissues to modulate host functions - contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplars and its genome encodes 1,184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana...
November 21, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27855160/transcription-factors-encoded-on-core-and-accessory-chromosomes-of-fusarium-oxysporum-induce-expression-of-effector-genes
#3
H Charlotte van der Does, Like Fokkens, Ally Yang, Sarah M Schmidt, Léon Langereis, Joanna M Lukasiewicz, Timothy R Hughes, Martijn Rep
Proteins secreted by pathogens during host colonization largely determine the outcome of pathogen-host interactions and are commonly called 'effectors'. In fungal plant pathogens, coordinated transcriptional up-regulation of effector genes is a key feature of pathogenesis and effectors are often encoded in genomic regions with distinct repeat content, histone code and rate of evolution. In the tomato pathogen Fusarium oxysporum f. sp. lycopersici (Fol), effector genes reside on one of four accessory chromosomes, known as the 'pathogenicity' chromosome, which can be exchanged between strains through horizontal transfer...
November 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27855158/high-resolution-genetics-identifies-the-lipid-transfer-protein-sec14p-as-target-for-antifungal-ergolines
#4
Ireos Filipuzzi, Simona Cotesta, Francesca Perruccio, Britta Knapp, Yue Fu, Christian Studer, Verena Pries, Ralph Riedl, Stephen B Helliwell, Katarina T Petrovic, N Rao Movva, Dominique Sanglard, Jianshi Tao, Dominic Hoepfner
Invasive infections by fungal pathogens cause more deaths than malaria worldwide. We found the ergoline compound NGx04 in an antifungal screen, with selectivity over mammalian cells. High-resolution chemogenomics identified the lipid transfer protein Sec14p as the target of NGx04 and compound-resistant mutations in Sec14p define compound-target interactions in the substrate binding pocket of the protein. Beyond its essential lipid transfer function in a variety of pathogenic fungi, Sec14p is also involved in secretion of virulence determinants essential for the pathogenicity of fungi such as Cryptococcus neoformans, making Sec14p an attractive antifungal target...
November 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27836848/investigation-of-the-role-played-by-cellobiose-dehydrogenases-from-podospora-anserina-during-lignocellulose-degradation
#5
Narumon Tangthirasunun, David Navarro, Sona Garajova, Didier Chevret, Laetitia Chan Ho Tong, Valérie Gautier, Kevin D Hyde, Philippe Silar, Jean-Guy Berrin
: Conversion of biomass into high-value products including biofuels is of great interest to develop sustainable biorefineries. Fungi are an inexhaustible source of enzymes to degrade plant biomass. Cellobiose dehydrogenases (CDHs) play an important role in the breakdown through synergistic action with fungal lytic polysaccharide monooxygenases (LPMOs). The three CDH genes of the model fungus Podospora anserina were inactivated resulting in singly and multiple CDH mutants. We detected almost no difference in the growth and fertility of the mutants on various lignocellulose sources, except on crystalline cellulose, where a two-fold decrease in fertility of the mutants lacking PaCDH1 and PaCDH2 was observed...
November 11, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27833621/transcriptional-basis-of-drought-induced-susceptibility-to-the-rice-blast-fungus-magnaporthe-oryzae
#6
Przemyslaw Bidzinski, Elsa Ballini, Aurélie Ducasse, Corinne Michel, Paola Zuluaga, Annamaria Genga, Remo Chiozzotto, Jean-Benoit Morel
Plants are often facing several stresses simultaneously. Understanding how they react and the way pathogens adapt to such combinational stresses is poorly documented. Here, we developed an experimental system mimicking field intermittent drought on rice followed by inoculation by the pathogenic fungus Magnaporthe oryzae. This experimental system triggers an enhancement of susceptibility that could be correlated with the dampening of several aspects of plant immunity, namely the oxidative burst and the transcription of several pathogenesis-related genes...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27822542/rna-sequencing-based-genome-reannotation-of-the-dermatophyte-arthroderma-benhamiae-and-characterization-of-its-secretome-and-whole-gene-expression-profile-during-infection
#7
Van Du T Tran, Niccolò De Coi, Marc Feuermann, Emanuel Schmid-Siegert, Elena-Tatiana Băguţ, Bernard Mignon, Patrice Waridel, Corinne Peter, Sylvain Pradervand, Marco Pagni, Michel Monod
Dermatophytes are the most common agents of superficial mycoses in humans and animals. The aim of the present investigation was to systematically identify the extracellular, possibly secreted, proteins that are putative virulence factors and antigenic molecules of dermatophytes. A complete gene expression profile of Arthroderma benhamiae was obtained during infection of its natural host (guinea pig) using RNA sequencing (RNA-seq) technology. This profile was completed with those of the fungus cultivated in vitro in two media containing either keratin or soy meal protein as the sole source of nitrogen and in Sabouraud medium...
July 2016: MSystems
https://www.readbyqxmd.com/read/27816794/investigating-the-cell-biology-of-plant-infection-by-the-rice-blast-fungus-magnaporthe-oryzae
#8
REVIEW
Xia Yan, Nicholas J Talbot
Rice blast disease is a major constraint on worldwide rice production and understanding the biology of plant infection is a priority for development of new disease control strategies. Recent advances in live cell imaging, coupled with tractability of both host and pathogen to molecular genetics and genomics, has made the rice blast pathosystem an important model for understanding plant disease. Here we review recent advances in understanding the cell biology of plant infection and, in particular, the remarkable ability of the rice blast fungus to invade plant tissue and manipulate the host plant using a battery of secreted effector proteins...
November 3, 2016: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/27809762/transcriptome-analysis-of-the-fungal-pathogen-fusarium-oxysporum-f-sp-medicaginis-during-colonisation-of-resistant-and-susceptible-medicago-truncatula-hosts-identifies-differential-pathogenicity-profiles-and-novel-candidate-effectors
#9
Louise F Thatcher, Angela H Williams, Gagan Garg, Sally-Anne G Buck, Karam B Singh
BACKGROUND: Pathogenic members of the Fusarium oxysporum species complex are responsible for vascular wilt disease on many important crops including legumes, where they can be one of the most destructive disease causing necrotrophic fungi. We previously developed a model legume-infecting pathosystem based on the reference legume Medicago truncatula and a pathogenic F. oxysporum forma specialis (f. sp.) medicaginis (Fom). To dissect the molecular pathogenicity arsenal used by this root-infecting pathogen, we sequenced its transcriptome during infection of a susceptible and resistant host accession...
November 3, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27796094/analysis-of-the-biotechnological-potential-of-a-lentinus-crinitus-isolate-in-the-light-of-its-secretome
#10
Geison Cambri, Mirta Mittelstedt Leal de Sousa, Davi de Miranda Fonseca, Fabricio Marchini, Joana Lea Meira da Silveira, Jaime Paba
Analysis of fungal secretomes is a prospection tool for the discovery of new catalysts with biotechnological application. Since enzyme secretion is strongly modulated by environmental factors, evaluation of growth conditions is of utmost importance to achieve optimal enzyme production. Here, a non-sequenced wood-rotting fungus, L. crinitus, was used for secretome analysis by enzymatic assays and a proteomics approach. Enzyme production was assessed after culturing the fungus in five different carbon sources and three nitrogen containing compounds...
October 31, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27766430/transcriptional-profiling-of-arbuscular-mycorrhizal-roots-exposed-to-high-levels-of-phosphate-reveals-the-repression-of-cell-cycle-related-genes-and-secreted-protein-genes-in-rhizophagus-irregularis
#11
Yusaku Sugimura, Katsuharu Saito
The development of arbuscular mycorrhiza (AM) is strongly suppressed under high-phosphate (Pi) conditions. To investigate AM fungal responses during the suppression of AM by high Pi, we performed an RNA-seq analysis of Rhizophagus irregularis colonizing Lotus japonicus roots at different levels of Pi (20, 100, 300, and 500 μM). AM fungal colonization decreased markedly under high-Pi conditions. In total, 163 fungal genes were differentially expressed among the four Pi treatments. Among these genes, a cell cycle-regulatory gene, cyclin-dependent kinase CDK1, and several DNA replication- and mitosis-related genes were repressed under high-Pi conditions...
October 20, 2016: Mycorrhiza
https://www.readbyqxmd.com/read/27764242/secreted-alpha-n-arabinofuranosidase-b-protein-is-required-for-the-full-virulence-of-magnaporthe-oryzae-and-triggers-host-defences
#12
Jingni Wu, Yiming Wang, Sook-Young Park, Sang Gon Kim, Ju Soon Yoo, Sangryeol Park, Ravi Gupta, Kyu Young Kang, Sun Tae Kim
Rice blast disease caused by Magnaporthe oryzae is one of the most devastating fungal diseases of rice and results in a huge loss of rice productivity worldwide. During the infection process, M. oryzae secretes a large number of glycosyl hydrolase proteins into the host apoplast to digest the cell wall and facilitate fungal ingression into host tissues. In this study, we identified a novel arabinofuranosidase-B (MoAbfB) protein that is secreted by M. oryzae during fungal infection. Deletion of MoAbfB from M...
2016: PloS One
https://www.readbyqxmd.com/read/27744264/involvement-of-the-fusarium-graminearum-cerato-platanin-proteins-in-fungal-growth-and-plant-infection
#13
Alessandra Quarantin, Anika Glasenapp, Wilhelm Schäfer, Francesco Favaron, Luca Sella
The genome of Fusarium graminearum, a necrotrophic fungal pathogen causing Fusarium head blight (FHB) disease of wheat, barley and other cereal grains, contains five genes putatively encoding for proteins with a cerato-platanin domain. Cerato-platanins are small secreted cysteine-rich proteins possibly localized in the fungal cell walls and also contributing to the virulence. Two of these F. graminearum proteins (FgCPP1 and FgCPP2) belong to the class of SnodProt proteins which exhibit phytotoxic activity in the fungal pathogens Botrytis cinerea and Magnaporthe grisea...
October 4, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27743115/an-in-vitro-model-for-the-study-of-the-macrophage-response-upon-trichophyton-rubrum-challenge
#14
Fabio Seiti Yamada Yoshikawa, Lucas Gonçalves Ferreira, Fernando Gonçalves de Almeida, Sandro Rogerio de Almeida
Diversity in the macrophage models currently employed in immunology studies may lead to opposed results and interpretations. In this study, we aimed to analyze the suitability of J774 macrophage-like cells as a model for the interaction between the dermatophyte Trichophyton rubrum and macrophages. J774 cells were competent in fungal phagocytosis, but succumbed to hyphal growth. Nevertheless, they could also secrete IL-1β in response to the dermatophyte. On the opposite direction, inflammatory, thioglycollate-induced peritoneal macrophages did not succumb to fungal growth and showed no significant IL-1β production...
October 14, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27739446/penicillium-arizonense-a-new-genome-sequenced-fungal-species-reveals-a-high-chemical-diversity-in-secreted-metabolites
#15
Sietske Grijseels, Jens Christian Nielsen, Milica Randelovic, Jens Nielsen, Kristian Fog Nielsen, Mhairi Workman, Jens Christian Frisvad
A new soil-borne species belonging to the Penicillium section Canescentia is described, Penicillium arizonense sp. nov. (type strain CBS 141311(T) = IBT 12289(T)). The genome was sequenced and assembled into 33.7 Mb containing 12,502 predicted genes. A phylogenetic assessment based on marker genes confirmed the grouping of P. arizonense within section Canescentia. Compared to related species, P. arizonense proved to encode a high number of proteins involved in carbohydrate metabolism, in particular hemicellulases...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27736862/genetic-determinism-of-sensitivity-to-corynespora-cassiicola-exudates-in-rubber-tree-hevea-brasiliensis
#16
Dinh Minh Tran, André Clément-Demange, Marine Déon, Dominique Garcia, Vincent Le Guen, Anne Clément-Vidal, Mouman Soumahoro, Aurélien Masson, Philippe Label, Mau Tuy Le, Valérie Pujade-Renaud
An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to Corynespora Leaf Fall (CLF) disease caused by the ascomycete Corynespora cassiicola. This method consists in quantifying the impact of fungal exudates on detached leaves by measuring the induced electrolyte leakage (EL%). The tested exudates were either crude culture filtrates from diverse C. cassiicola isolates or the purified cassiicolin (Cas1), a small secreted effector protein produced by the aggressive isolate CCP...
2016: PloS One
https://www.readbyqxmd.com/read/27716942/an-assay-for-entry-of-secreted-fungal-effectors-into-plant-cells
#17
Libera Lo Presti, Bernd Zechmann, Jochen Kumlehn, Liang Liang, Daniel Lanver, Shigeyuki Tanaka, Ralph Bock, Regine Kahmann
Successful colonization of plants by prokaryotic and eukaryotic pathogens requires active effector-mediated suppression of defense responses and host tissue reprogramming. Secreted effector proteins can either display their activity in the apoplast or translocate into host cells and function therein. Although characterized in bacteria, the molecular mechanisms of effector delivery by fungal phytopathogens remain elusive. Here we report the establishment of an assay that is based on biotinylation of effectors in the host cytoplasm as hallmark of uptake...
September 26, 2016: New Phytologist
https://www.readbyqxmd.com/read/27716291/enzymatic-fine-tuning-for-2-6-hydroxynaphthyl-%C3%AE-d-xylopyranoside-synthesis-catalyzed-by-the-recombinant-%C3%AE-xylosidase-bxtw1-from-talaromyces-amestolkiae
#18
Manuel Nieto-Domínguez, Alicia Prieto, Beatriz Fernández de Toro, Francisco Javier Cañada, Jorge Barriuso, Zach Armstrong, Stephen G Withers, Laura I de Eugenio, María Jesús Martínez
BACKGROUND: Glycosides are compounds displaying crucial biological roles and plenty of applications. Traditionally, these molecules have been chemically obtained, but its efficient production is limited by the lack of regio- and stereo-selectivity of the chemical synthesis. As an interesting alternative, glycosidases are able to catalyze the formation of glycosides in a process considered green and highly selective. In this study, we report the expression and characterization of a fungal β-xylosidase in Pichia pastoris...
October 4, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27711117/de-novo-analysis-of-the-epiphytic-transcriptome-of-the-cucurbit-powdery-mildew-fungus-podosphaera-xanthii-and-identification-of-candidate-secreted-effector-proteins
#19
David Vela-Corcía, Rocío Bautista, Antonio de Vicente, Pietro D Spanu, Alejandro Pérez-García
The cucurbit powdery mildew fungus Podosphaera xanthii is a major limiting factor for cucurbit production worldwide. Despite the fungus's agronomic and economic importance, very little is known about fundamental aspects of P. xanthii biology, such as obligate biotrophy or pathogenesis. To design more durable control strategies, genomic information about P. xanthii is needed. Powdery mildews are fungal pathogens with large genomes compared with those of other fungi, which contain vast amounts of repetitive DNA sequences, much of which is composed of retrotransposons...
2016: PloS One
https://www.readbyqxmd.com/read/27703209/a-novel-exopolysaccharide-elicitor-from-endophytic-fungus-gilmaniella-sp-al12-on-volatile-oils-accumulation-in-atractylodes-lancea
#20
Fei Chen, Cheng-Gang Ren, Tong Zhou, Yu-Jia Wei, Chuan-Chao Dai
Endophytes and plants can establish specific long-term symbiosis through the accumulation of secondary metabolites. Previous studies have shown that the endophytic fungus Gilmaniella sp. AL12 can stimulate Atractylodes lancea to produce volatile oils. The purpose of this report is to investigate key factors involved in the stimulation of A. lancea by AL12 and reveal the mechanism. We identified the active component from AL12 as an extracellular mannan with a polymerization degree of 26-42. Differential membrane proteomics of A...
October 5, 2016: Scientific Reports
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