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fungi secreted protein

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https://www.readbyqxmd.com/read/28223991/the-comparison-of-expressed-candidate-secreted-proteins-from-two-arbuscular-mycorrhizal-fungi-unravels-common-and-specific-molecular-tools-to-invade-different-host-plants
#1
Laurent Kamel, Nianwu Tang, Mathilde Malbreil, Hélène San Clemente, Morgane Le Marquer, Christophe Roux, Nicolas Frei Dit Frey
Arbuscular mycorrhizal fungi (AMF), belonging to the fungal phylum Glomeromycota, form mutualistic symbioses with roots of almost 80% of land plants. The release of genomic data from the ubiquitous AMF Rhizophagus irregularis revealed that this species possesses a large set of putative secreted proteins (RiSPs) that could be of major importance for establishing the symbiosis. In the present study, we aimed to identify SPs involved in the establishment of AM symbiosis based on comparative gene expression analyses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28215348/in%C3%A2-vitro-secretomic-analysis-identifies-putative-pathogenicity-related-proteins-of-sporisorium-scitamineum-the-sugarcane-smut-fungus
#2
Leonard Barnabas, N M R Ashwin, Kalimuthu Kaverinathan, Anna Rita Trentin, Micaela Pivato, Amalraj Ramesh Sundar, Palaniyandi Malathi, Rasappa Viswanathan, Paolo Carletti, Giorgio Arrigoni, Antonio Masi, Ganesh Kumar Agrawal, Randeep Rakwal
Sporisorium scitamineum, the sugarcane smut pathogen, relies predominantly on its secretome to successfully colonise its host, in accordance with other related smut fungi. Considering the significance of deciphering its secretome, we have examined alterations in the in vitro secretome of S. scitamineum in response to synthetic and sugarcane meristem tissue-amended growth media, so as to identify host signal responsive secretory proteins. Secretory proteins that were differentially abundant and exclusively secreted in response to host extract media were identified by two-dimensional gel electrophoresis coupled with MALDI-TOF/TOF MS...
March 2017: Fungal Biology
https://www.readbyqxmd.com/read/28168773/characterization-of-an-entomopathogenic-fungi-target-integument-protein-bombyx-mori-single-domain-von-willebrand-factor-type-c-in-the-silkworm-bombyx-mori
#3
F Han, A Lu, Y Yuan, W Huang, B T Beerntsen, J Huang, E Ling
The insect cuticle works as the first line of defence to protect insects from pathogenic infections and water evaporation. However, the old cuticle must be shed in order to enter the next developmental stage. During each ecdysis, moulting fluids are produced and secreted into the area among the old and new cuticles. In a previous study, the protein Bombyx mori single domain von Willebrand factor type C (BmSVWC; BGIBMGA011399) was identified in the moulting fluids of Bo. mori and demonstrated to regulate ecdysis...
February 7, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28137563/evaluating-exosome-protein-content-changes-induced-by-virus-activity-using-silac-labeling-and-lc-ms-ms
#4
X Zhao, Y Xie, J Liu
Exosomes are small membrane vesicles that are produced by cells and excreted into extracellular space. Contents of exosomes generally include lipid, membrane, and soluble proteins, and various types of coding and noncoding RNAs. Over the past decades, it has become clear that exosomes constitute an important vector for intercellular transport and communication with significant functional relevance. Evaluating exosome contents and their changes are vital for understanding its role in different physiological and pathological processes...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28130302/distinct-growth-and-secretome-strategies-by-two-taxonomically-divergent-brown-rot-fungi
#5
Gerald N Presley, Jonathan S Schilling
: Brown rot fungi are wood-degrading fungi that employ both oxidative and hydrolytic mechanisms to degrade wood. Hydroxyl radicals that facilitate the oxidative component are powerful non-selective oxidants and are incompatible with hydrolytic enzymes unless they are spatially segregated in wood. Differential gene expression has been implicated in Postia placenta to segregate these reactions, but it is unclear if this two-step mechanism varies in other brown rot fungi with different traits and life history strategies, and that occupy different niches in nature...
January 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28103392/a-peroxygenase-from-chaetomium-globosum-catalyzes-the-selective-oxygenation-of-testosterone
#6
Jan Kiebist, Kai-Uwe Schmidtke, Jörg Zimmermann, Harald Kellner, Nico Jehmlich, René Ullrich, Daniel Zänder, Martin Hofrichter, Katrin Scheibner
Unspecific peroxygenases (UPO, EC 1.11.2.1) secreted by fungi open an efficient way to selectively oxyfunctionalize diverse organic substrates including less activated hydrocarbons by transferring peroxide-borne oxygen. Herein, we investigated a cell-free approach to incorporate epoxy and hydroxyl functionalities directly into the bulky molecule of testosterone by a novel unspecific peroxygenase that was produced by the ascomycetous fungus Chaetomium globosum in a complex medium rich in carbon and nitrogen...
January 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28096492/identification-of-glutaminyl-cyclase-genes-involved-in-pyroglutamate-modification-of-fungal-lignocellulolytic-enzymes
#7
Vincent W Wu, Craig M Dana, Anthony T Iavarone, Douglas S Clark, N Louise Glass
: The breakdown of plant biomass to simple sugars is essential for the production of second-generation biofuels and high-value bioproducts. Currently, enzymes produced from filamentous fungi are used for deconstructing plant cell wall polysaccharides into fermentable sugars for biorefinery applications. A post-translational N-terminal pyroglutamate modification observed in some of these enzymes occurs when N-terminal glutamine or glutamate is cyclized to form a five-membered ring. This modification has been shown to confer resistance to thermal denaturation for CBH-1 and EG-1 cellulases...
January 17, 2017: MBio
https://www.readbyqxmd.com/read/28089933/hfb7-a-novel-orphan-hydrophobin-of-the-harzianum-and-virens-clades-of-trichoderma-is-involved-in-response-to-biotic-and-abiotic-stresses
#8
Agnes Przylucka, Gunseli Bayram Akcapinar, Komal Chenthamara, Feng Cai, Marica Grujuc, Juriy Karpenko, Miriam Livoi, Qirong Shen, Christian P Kubicek, Irina S Druzhinina
Hydrophobins are small secreted cysteine-rich proteins exclusively found in fungi. They are able to self-assemble in single molecular layers at hydrophobic-hydrophilic interfaces and can therefore be directly involved in establishment of fungi in their habitat. The genomes of filamentous mycotrophic fungi Trichoderma encode a rich diversity of hydrophobins, which are divided in several groups based on their structure and evolution. Here we describe a new member of class II hydrophobins, HFB7, that has a taxonomically restricted occurrence in Harzianum and Virens clades of Trichoderma...
January 12, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28089076/comparative-analyses-of-secreted-proteins-in-plant-pathogenic-smut-fungi-and-related-basidiomycetes
#9
Mariana Schuster, Gabriel Schweizer, Regine Kahmann
In the ten years since the genome sequence of the basidiomycete corn smut fungus Ustilago maydis was published, additional genomes of smut species infecting different hosts became available. In addition, the genomes of related Malassezia species causing skin diseases and of Pseudozyma species not known to infect plants were determined. As secreted proteins are critical virulence determinants in U. maydis we compare here the secretomes of 12 basidiomycete species to gain information about their composition and conservation...
January 6, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28087464/mitochondrial-reactive-oxygen-species-regulate-fungal-protease-induced-inflammatory-responses
#10
Yun Hee Kim, Seung-Hyo Lee
Epidemiological studies have shown that fungal infections are a main cause of respiratory tract diseases, such as asthma, bronchopneumonia, intoxication, and invasive fungal disease. Fungi such as Aspergillus and Candida species have become increasingly important pathogens as the global climate changes. Accordingly, in this study, we evaluated the toxicological potential of Aspergillus protease in the lower respiratory tract. Exposure of Aspergillus protease to A549 cells induced upregulation of tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP)-1, and intercellular adhesion molecule (ICAM)-1 mRNAs and increased production of interleukin (IL)-8 and MCP-1 protein through enhanced mitochondrial reactive oxygen species (ROS) generation and activation of mitogen-activated protein kinase (MAPK) and activator protein (AP)-1...
January 10, 2017: Toxicology
https://www.readbyqxmd.com/read/28074041/transcription-factor-xpp1-is-a-switch-between-primary-and-secondary-fungal-metabolism
#11
Christian Derntl, Bernhard Kluger, Christoph Bueschl, Rainer Schuhmacher, Robert L Mach, Astrid R Mach-Aigner
Fungi can produce a wide range of chemical compounds via secondary metabolism. These compounds are of major interest because of their (potential) application in medicine and biotechnology and as a potential source for new therapeutic agents and drug leads. However, under laboratory conditions, most secondary metabolism genes remain silent. This circumstance is an obstacle for the production of known metabolites and the discovery of new secondary metabolites. In this study, we describe the dual role of the transcription factor Xylanase promoter binding protein 1 (Xpp1) in the regulation of both primary and secondary metabolism of Trichoderma reesei Xpp1 was previously described as a repressor of xylanases...
January 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28069559/proteomic-insights-into-mannan-degradation-and-protein-secretion-by-the-forest-floor-bacterium-chitinophaga-pinensis
#12
Johan Larsbrink, Tina R Tuveng, Phillip B Pope, Vincent Bulone, Vincent G H Eijsink, Harry Brumer, Lauren S McKee
: Together with fungi, saprophytic bacteria are central to the decomposition and recycling of biomass in forest environments. The Bacteroidetes phylum is abundant in diverse habitats, and several species have been shown to be able to deconstruct a wide variety of complex carbohydrates. The genus Chitinophaga is often enriched in hotspots of plant and microbial biomass degradation. We present a proteomic assessment of the ability of Chitinophaga pinensis to grow on and degrade mannan polysaccharides, using an agarose plate-based method of protein collection to minimise contamination with exopolysaccharides and proteins from lysed cells, and to reflect the realistic setting of growth on a solid surface...
March 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28066872/comparative-genomics-and-expression-levels-of-hydrophobins-from-eight-mycorrhizal-genomes
#13
F Rineau, H Lmalem, D Ahren, F Shah, T Johansson, L Coninx, J Ruytinx, H Nguyen, I Grigoriev, A Kuo, A Kohler, E Morin, J Vangronsveld, F Martin, J V Colpaert
Hydrophobins are small secreted proteins that are present as several gene copies in most fungal genomes. Their properties are now well understood: they are amphiphilic and assemble at hydrophilic/hydrophobic interfaces. However, their physiological functions remain largely unexplored, especially within mycorrhizal fungi. In this study, we identified hydrophobin genes and analysed their distribution in eight mycorrhizal genomes. We then measured their expression levels in three different biological conditions (mycorrhizal tissue vs...
January 9, 2017: Mycorrhiza
https://www.readbyqxmd.com/read/28064367/cellular-responses-to-the-expression-of-unstable-secretory-proteins-in-the-filamentous-fungus-aspergillus-oryzae
#14
Jun-Ichi Yokota, Daisuke Shiro, Mizuki Tanaka, Yasumichi Onozaki, Osamu Mizutani, Dararat Kakizono, Sakurako Ichinose, Tomoko Shintani, Katsuya Gomi, Takahiro Shintani
Filamentous fungi are often used as cell factories for recombinant protein production because of their ability to secrete large quantities of hydrolytic enzymes. However, even using strong transcriptional promoters, yields of nonfungal proteins are generally much lower than those of fungal proteins. Recent analyses revealed that expression of certain nonfungal secretory proteins induced the unfolded protein response (UPR), suggesting that they are recognized as proteins with folding defects in filamentous fungi...
January 7, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28063828/genome-wide-computational-analysis-of-the-secretome-of-brown-algae-phaeophyceae
#15
Makoto Terauchi, Takahiro Yamagishi, Takeaki Hanyuda, Hiroshi Kawai
Brown algae have evolved complex multicellularity in the heterokont lineage. They are phylogenetically distant to land plants, fungi and animals. Especially, the members of Laminariales (so-called kelps) have developed highly differentiated tissues. Extracellular matrix (ECM) plays pivotal roles in a number of essential processes in multicellular organisms, such as cell adhesion, cell and tissue differentiations, cell-to-cell communication, and responses to environmental stimuli. In these processes, a set of extracellular secreted proteins called the secretome operates remodeling of the physicochemical nature of ECM and signal transduction by interacting with cell surface proteins and signaling molecules...
January 4, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28062375/casein-phosphopeptides-and-cacl2-increase-penicillin-production-and-cause-an-increment-in-microbody-peroxisome-proteins-in-penicillium-chrysogenum
#16
Rebeca Domínguez-Santos, Katarina Kosalková, Carlos García-Estrada, Carlos Barreiro, Ana Ibáñez, Alejandro Morales, Juan-Francisco Martín
: Transport of penicillin intermediates and penicillin secretion are still poorly characterized in Penicillium chrysogenum (re-identified as Penicillium rubens). Calcium (Ca(2+)) plays an important role in the metabolism of filamentous fungi, and casein phosphopeptides (CPP) are involved in Ca(2+) internalization. In this study we observe that the effect of CaCl2 and CPP is additive and promotes an increase in penicillin production of up to 10-12 fold. Combination of CaCl2 and CPP greatly promotes expression of the three penicillin biosynthetic genes...
March 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28053662/development-of-a-genome-editing-crispr-cas9-system-in-thermophilic-fungal-myceliophthora-species-and-its-application-to-hyper-cellulase-production-strain-engineering
#17
Qian Liu, Ranran Gao, Jingen Li, Liangcai Lin, Junqi Zhao, Wenliang Sun, Chaoguang Tian
BACKGROUND: Over the past 3 years, the CRISPR/Cas9 system has revolutionized the field of genome engineering. However, its application has not yet been validated in thermophilic fungi. Myceliophthora thermophila, an important thermophilic biomass-degrading fungus, has attracted industrial interest for the production of efficient thermostable enzymes. Genetic manipulation of Myceliophthora is crucial for metabolic engineering and to unravel the mechanism of lignocellulose deconstruction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28051166/identification-of-small-rnas-in-extracellular-vesicles-from-the-commensal-yeast-malassezia-sympodialis
#18
Simon Rayner, Sören Bruhn, Helen Vallhov, Anna Andersson, R Blake Billmyre, Annika Scheynius
Malassezia is the dominant fungus in the human skin mycobiome and is associated with common skin disorders including atopic eczema (AE)/dermatitis. Recently, it was found that Malassezia sympodialis secretes nanosized exosome-like vesicles, designated MalaEx, that carry allergens and can induce inflammatory cytokine responses. Extracellular vesicles from different cell-types including fungi have been found to deliver functional RNAs to recipient cells. In this study we assessed the presence of small RNAs in MalaEx and addressed if the levels of these RNAs differ when M...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28039549/expression-of-talaromyces-thermophilus-lipase-gene-in-trichoderma-reesei-by-homologous-recombination-at-the-cbh1-locus
#19
Xu Zhang, Liming Xia
CBH1 (cellobiohydrolase) comprises the majority of secreted proteins by Trichoderma reesei. For expression of Talaromyces thermophilus lipase gene in T. reesei, a self-designed CBH1 promoter was applied to drive the lipase gene expression cassette which was bracketed by flanking sequences of cbh1 gene for homologous recombination. Protoplast and Agrobacterium-mediated plasmid transformations were performed and compared, resultantly, transformation mediated by Agrobacterium was overall proved to be more efficient...
December 30, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28039134/a-family-of-secretory-proteins-is-associated-with-different-morphotypes-in-cryptococcus-neoformans
#20
Rachana Gyawali, Srijana Upadhyay, Joshua Way, Xiaorong Lin
Cryptococcus neoformans, an opportunistic human fungal pathogen, can undergo a yeast-to-hypha transition in response to environmental cues. This morphological transition is associated with changes in the expression of cell surface proteins. The Cryptococcus cell surface and secreted protein Cfl1 was the first identified adhesin in the Basidiomycota. Cfl1 has been shown to regulate morphology, biofilm formation, and intercellular communication. Four additional homologs of CFL1 are harbored by the Cryptococcus genome: DHA1, DHA2, CPL1, and CFL105 The common features of this gene family are the conserved C-terminal SIGC domain and the presence of an N-terminal signal peptide...
March 1, 2017: Applied and Environmental Microbiology
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