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fungi secreted protein

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https://www.readbyqxmd.com/read/29145801/comparative-genomics-of-coniophora-olivacea-reveals-different-patterns-of-genome-expansion-in-boletales
#1
Raúl Castanera, Gúmer Pérez, Leticia López-Varas, Joëlle Amselem, Kurt LaButti, Vasanth Singan, Anna Lipzen, Sajeet Haridas, Kerrie Barry, Igor V Grigoriev, Antonio G Pisabarro, Lucía Ramírez
BACKGROUND: Coniophora olivacea is a basidiomycete fungus belonging to the order Boletales that produces brown-rot decay on dead wood of conifers. The Boletales order comprises a diverse group of species including saprotrophs and ectomycorrhizal fungi that show important differences in genome size. RESULTS: In this study we report the 39.07-megabase (Mb) draft genome assembly and annotation of C. olivacea. A total of 14,928 genes were annotated, including 470 putatively secreted proteins enriched in functions involved in lignocellulose degradation...
November 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29144203/suppression-or-activation-of-immune-responses-by-predicted-secreted-proteins-of-the-soybean-rust-pathogen-phakopsora-pachyrhizi
#2
Mingsheng Qi, James P Grayczyk, Janina M Seitz, Youngsill Lee, Tobias I Link, Doil Choi, Kerry F Pedley, Ralf T Voegele, Thomas J Baum, Steven A Whitham
Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization...
November 16, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29131484/the-major-cellulases-cbh-1-and-cbh-2-of-neurospora-crassa-rely-on-distinct-er-cargo-adaptors-for-efficient-er-exit
#3
Trevor Starr, A Pedro Gonçalves, Neeka Meshgin, N Louise Glass
Filamentous fungi are native secretors of lignocellulolytic enzymes and are used as protein-producing factories in the industrial biotechnology sector. Despite the importance of these organisms in industry, relatively little is known about the filamentous fungal secretory pathway or how it might be manipulated for improved protein production. Here we use Neurospora crassa as a model filamentous fungus to interrogate the requirements for trafficking of cellulase enzymes from the endoplasmic reticulum to the Golgi...
November 13, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29126189/comparative-genome-analysis-reveals-adaptation-to-the-ectophytic-lifestyle-of-sooty-blotch-and-flyspeck-fungi
#4
Chao Xu, Rong Zhang, Guangyu Sun, Mark L Gleason
Sooty blotch and flyspeck (SBFS) fungi are a distinctive group of plant pathogens which, although phylogenetically diverse, occupy an exclusively surface-dwelling niche. They cause economic losses by superficially blemishing the fruit of several tree crops, principally apple, in moist temperate regions worldwide. In this study, we performed genome-wide comparative analyses separately within three pairs of species of ascomycete pathogens; each pair contained an SBFS species as well as a closely related but plant-penetrating parasite (PPP) species...
November 6, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29119000/comparing-the-physiochemical-parameters-of-three-celluloses-reveals-new-insights-into-substrate-suitability-for-fungal-enzyme-production
#5
Lara Hassan, Manfred J Reppke, Nils Thieme, Steffen A Schweizer, Carsten W Mueller, J Philipp Benz
Background: The industrial applications of cellulases are mostly limited by the costs associated with their production. Optimized production pathways are therefore desirable. Based on their enzyme inducing capacity, celluloses are commonly used in fermentation media. However, the influence of their physiochemical characteristics on the production process is not well understood. In this study, we examined how physical, structural and chemical properties of celluloses influence cellulase and hemicellulase production in an industrially-optimized and a non-engineered filamentous fungus: Trichoderma reesei RUT-C30 and Neurospora crassa...
2017: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29116156/efficient-production-and-characterization-of-the-novel-and-highly-active-antifungal-protein-afpb-from-penicillium-digitatum
#6
Sandra Garrigues, Mónica Gandía, Crina Popa, Attila Borics, Florentine Marx, María Coca, Jose F Marcos, Paloma Manzanares
Filamentous fungi encode distinct antifungal proteins (AFPs) that offer great potential to develop new antifungals. Fungi are considered immune to their own AFPs as occurs in Penicillium chrysogenum, the producer of the well-known PAF. The Penicillium digitatum genome encodes only one afp gene (afpB), and the corresponding protein (AfpB) belongs to the class B phylogenetic cluster. Previous attempts to detect AfpB were not successful. In this work, immunodetection confirmed the absence of AfpB accumulation in wild type and previous recombinant constitutive P...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29109463/a-role-for-small-secreted-proteins-ssps-in-a-saprophytic-fungal-lifestyle-ligninolytic-enzyme-regulation-in-pleurotus-ostreatus
#7
Daria Feldman, David J Kowbel, N Louise Glass, Oded Yarden, Yitzhak Hadar
Small secreted proteins (SSPs), along with lignocellulose degrading enzymes, are integral components of the secretome of Pleurotus ostreatus, a white rot fungus. In this study, we identified 3 genes (ssp1, 2 and 3) encoding proteins that are annotated as SSPs and that exhibited of ~4,500- fold expression, 24 hr following exposure to the toxic compound 5-hydroxymethylfurfural (HMF). Homologues to genes encoding these SSPs are present in the genomes of other basidiomycete fungi, however the role of SSPs is not yet understood...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29105533/plant-immunity-and-symbiosis-signaling-mediated-by-lysm-receptors
#8
Yoshitake Desaki, Kana Miyata, Maruya Suzuki, Naoto Shibuya, Hanae Kaku
Plants possess the ability to recognize microbe-associated molecular patterns (MAMPs) and PAMPs through the PRRs, and initiate pattern-triggered immunity. MAMPs are derived from cell-envelope components, secreted materials and cytosolic proteins from bacteria, oomycetes or fungi, and some MAMPs play a similar function in the innate immunity in mammals. Chitin is a representative fungal MAMP and triggers defense signaling in a wide range of plant species. The chitin receptors CEBiP and CERK1 on the plasma membrane have LysM (lysin motif) in their ectodomains...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29076546/a-cerato-platanin-protein-sscp1-targets-plant-pr1-and-contributes-to-virulence-of-sclerotinia-sclerotiorum
#9
Guogen Yang, Liguang Tang, Yingdi Gong, Jiatao Xie, Yanping Fu, Daohong Jiang, Guoqing Li, David B Collinge, Weidong Chen, Jiasen Cheng
Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S...
October 27, 2017: New Phytologist
https://www.readbyqxmd.com/read/29062087/predicted-microbial-secretomes-and-their-target-substrates-in-marine-sediment
#10
William D Orsi, Thomas A Richards, Warren R Francis
Scientific drilling has identified a biosphere in marine sediments (1) , which contain many uncultivated microbial groups known only by their DNA sequences (2-4) . Recycling of organic matter in sediments is an important component of biogeochemical cycles because marine sediments are critical for long-term carbon storage (5) . Turnover of carbon is hypothesized to be driven by the secretion of enzymes by microbial organisms (5-7) , which act to break down macromolecules into constitutive monomers that can be transported into cells...
October 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/29043572/secreted-proteins-produced-by-fungi-associated-with-botryosphaeria-dieback-trigger-distinct-defense-responses-in-vitis-vinifera-and-vitis-rupestris-cells
#11
E Stempien, M-L Goddard, Y Leva, M Bénard-Gellon, H Laloue, S Farine, F Kieffer-Mazet, C Tarnus, C Bertsch, J Chong
Grapevine trunk diseases (Eutypa dieback, esca and Botryosphaeria dieback) are caused by a complex of xylem-inhabiting fungi, which severely reduce yields in vineyards. Botryosphaeria dieback is associated with Botryosphaeriaceae. In order to develop effective strategies against Botryosphaeria dieback, we investigated the molecular basis of grapevine interactions with a virulent species, Neofusicoccum parvum, and a weak pathogen, Diplodia seriata. We investigated defenses induced by purified secreted fungal proteins within suspension cells of Vitis (Vitis rupestris and Vitis vinifera cv...
October 17, 2017: Protoplasma
https://www.readbyqxmd.com/read/29038760/cellobiohydrolase-b-of-aspergillus-niger-over-expressed-in-pichia-pastoris-stimulates-hydrolysis-of-oil-palm-empty-fruit-bunches
#12
James Sy-Keen Woon, Mukram M Mackeen, Rosli M Illias, Nor M Mahadi, William J Broughton, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
BACKGROUND: Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-glucosidases, endoglucanases and cellobiohydrolases. Cellobiohydrolases (CBH) are vital to the degradation of crystalline cellulose present in lignocellulosic biomass. However, A. niger naturally secretes low levels of CBH. Hence, recombinant production of A...
2017: PeerJ
https://www.readbyqxmd.com/read/29020037/a-conserved-fungal-glycosyltransferase-facilitates-pathogenesis-of-plants-by-enabling-hyphal-growth-on-solid-surfaces
#13
Robert King, Martin Urban, Rebecca P Lauder, Nichola Hawkins, Matthew Evans, Amy Plummer, Kirstie Halsey, Alison Lovegrove, Kim Hammond-Kosack, Jason J Rudd
Pathogenic fungi must extend filamentous hyphae across solid surfaces to cause diseases of plants. However, the full inventory of genes which support this is incomplete and many may be currently concealed due to their essentiality for the hyphal growth form. During a random T-DNA mutagenesis screen performed on the pleomorphic wheat (Triticum aestivum) pathogen Zymoseptoria tritici, we acquired a mutant unable to extend hyphae specifically when on solid surfaces. In contrast "yeast-like" growth, and all other growth forms, were unaffected...
October 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28992048/comparative-whole-genome-analysis-reveals-artificial-selection-effects-on-ustilago-esculenta-genome
#14
Zihong Ye, Yao Pan, Yafen Zhang, Haifeng Cui, Gulei Jin, Alice C McHardy, Longjiang Fan, Xiaoping Yu
Ustilago esculenta, infects Zizania latifolia, and induced host stem swollen to be a popular vegetable called Jiaobai in China. It is the long-standing artificial selection that maximizes the occurrence of favourable Jiaobai, and thus maintaining the plant-fungi interaction and modulating the fungus evolving from plant pathogen to entophyte. In this study, whole genome of U. esculenta was sequenced and transcriptomes of the fungi and its host were analysed. The 20.2 Mb U. esculenta draft genome of 6,654 predicted genes including mating, primary metabolism, secreted proteins, shared a high similarity to related Smut fungi...
July 19, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28985305/genome-sequencing-and-comparative-genomics-reveal-the-potential-pathogenic-mechanism-of-cercospora-sojina-hara-on-soybean
#15
Xuming Luo, Jidong Cao, Junkai Huang, Zongyi Wang, Zhengyan Guo, Yihua Chen, Shumei Ma, Jun Liu
Frogeye leaf spot, caused by Cercospora sojina Hara, is a common disease of soybean in most soybean-growing countries of the world. In this study, we report a high-quality genome sequence of C. sojina by Single Molecule Real-Time sequencing method. The 40.8-Mb genome encodes 11,655 predicated genes, and 8,474 genes are revealed by RNA sequencing. Cercospora sojina genome contains large numbers of gene clusters that are involved in synthesis of secondary metabolites, including mycotoxins and pigments. However, much less carbohydrate-binding module protein encoding genes are identified in C...
September 18, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28981698/distinct-trajectories-of-massive-recent-gene-gains-and-losses-in-populations-of-a-microbial-eukaryotic-pathogen
#16
Fanny E Hartmann, Daniel Croll
Differences in gene content are a significant source of variability within species and have an impact on phenotypic traits. However, little is known about the mechanisms responsible for the most recent gene gains and losses. We screened the genomes of 123 worldwide isolates of the major pathogen of wheat Zymoseptoria tritici for robust evidence of gene copy number variation. Based on orthology relationships in three closely related fungi, we identified 599 gene gains and 1,024 gene losses that have not yet reached fixation within the focal species...
November 1, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28951729/regulatory-mechanisms-of-a-highly-pectinolytic-mutant-of-penicillium-occitanis-and-functional-analysis-of-a-candidate-gene-in-the-plant-pathogen-fusarium-oxysporum
#17
Gustavo Bravo-Ruiz, Azza Hadj Sassi, Marina Marcet-Houben, Antonio Di Pietro, Ali Gargouri, Toni Gabaldon, M Isabel G Roncero
Penicillium occitanis is a model system for enzymatic regulation. A mutant strain exhibiting constitutive overproduction of different pectinolytic enzymes both under inducing (pectin) or repressing conditions (glucose) was previously isolated after chemical mutagenesis. In order to identify the molecular basis of this regulatory mechanism, the genomes of the wild type and the derived mutant strain were sequenced and compared, providing the first reference genome for this species. We used a phylogenomic approach to compare P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28938005/recombinant-vaccines-of-a-cd4-t-cell-epitope-promote-efficient-control-of-paracoccidioides-brasiliensis-burden-by-restraining-primary-organ-infection
#18
Rodrigo Assunção Holanda, Julián Esteban Muñoz, Lucas Santos Dias, Leandro Buffoni Roque Silva, Julliana Ribeiro Alves Santos, Sthefany Pagliari, Érica Leandro Marciano Vieira, Tatiane Alves Paixão, Carlos Pelleschi Taborda, Daniel Assis Santos, Oscar Bruña-Romero
Paracoccidioidomycosis (PCM) is an infectious disease endemic to South America, caused by the thermally dimorphic fungi Paracoccidioides. Currently, there is no effective human vaccine that can be used in prophylactic or therapeutic regimes. We tested the hypothesis that the immunogenicity of the immunodominant CD4+ T-cell epitope (P10) of Paracoccidioides brasiliensis gp43 antigen might be significantly enhanced by using a hepatitis B virus-derived particle (VLP) as an antigen carrier. This chimera was administered to mice as a (His)6-purified protein (rPbT) or a replication-deficient human type 5 adenoviral vector (rAdPbT) in an immunoprophylaxis assay...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28932199/bacillus-a-biological-tool-for-crop-improvement-through-bio-molecular-changes-in-adverse-environments
#19
REVIEW
Ramalingam Radhakrishnan, Abeer Hashem, Elsayed F Abd Allah
Crop productivity is affected by environmental and genetic factors. Microbes that are beneficial to plants are used to enhance the crop yield and are alternatives to chemical fertilizers and pesticides. Pseudomonas and Bacillus species are the predominant plant growth-promoting bacteria. The spore-forming ability of Bacillus is distinguished from that of Pseudomonas. Members of this genus also survive for a long time under unfavorable environmental conditions. Bacillus spp. secrete several metabolites that trigger plant growth and prevent pathogen infection...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28923004/regulatory-networks-underlying-mycorrhizal-development-delineated-by-genome-wide-expression-profiling-and-functional-analysis-of-the-transcription-factor-repertoire-of-the-plant-symbiotic-fungus-laccaria-bicolor
#20
Y Daguerre, E Levati, J Ruytinx, E Tisserant, E Morin, A Kohler, B Montanini, S Ottonello, A Brun, C Veneault-Fourrey, F Martin
BACKGROUND: Ectomycorrhizal (ECM) fungi develop a mutualistic symbiotic interaction with the roots of their host plants. During this process, they undergo a series of developmental transitions from the running hyphae in the rhizosphere to the coenocytic hyphae forming finger-like structures within the root apoplastic space. These transitions, which involve profound, symbiosis-associated metabolic changes, also entail a substantial transcriptome reprogramming with coordinated waves of differentially expressed genes...
September 18, 2017: BMC Genomics
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