Read by QxMD icon Read

fungi secreted protein

Victor C Ujor, Emmanuel C Adukwu, Christopher C Okonkwo
Non-self contact between fungi elicits strong morphological and biochemical reactions in the mycelia of interacting species. Although these reactions appear to be species- and interaction-specific, some responses such as pigmentation, increased secretion of phenol-oxidases, barrage formation and sealing of the mycelia front are common responses in most interactions. Hence, some species recruit similar molecular machineries in response to non-self. Increasing number of fully sequenced and annotated fungal genomes and advances in genome-wide and global proteome analytical tools now allow researchers to use techniques such as RNA sequencing, micro and macroarray analysis, 2-dimensional protein gel profiling, and differential display of mRNA to probe the underlying molecular mechanisms of combative mycelial interactions...
April 2018: Fungal Biology
Leonardo Iula, Irene A Keitelman, Florencia Sabbione, Federico Fuentes, Mauricio Guzman, Jeremías Gastón Galletti, Pehuén Pereyra Gerber, Matías Ostrowski, Jorge R Geffner, Carolina C Jancic, Analía S Trevani
Interleukin-1β (IL-1β), a major pro-inflammatory cytokine, is a leaderless cytosolic protein whose secretion does not follow the classical endoplasmic reticulum-to-Golgi pathway, and for which a canonical mechanism of secretion remains to be established. Neutrophils are essential players against bacterial and fungi infections. These cells are rapidly and massively recruited from the circulation into infected tissues and, beyond of displaying an impressive arsenal of toxic weapons effective to kill pathogens, are also an important source of IL-1β in infectious conditions...
2018: Frontiers in Immunology
Yumeng Chen, Yaling Shen, Wei Wang, Dongzhi Wei
Background: The filamentous fungus Trichoderma reesei Rut-C30 is one of the most vital fungi for the production of cellulases, which can be used for biofuel production from lignocellulose. Nevertheless, the mechanism of transmission of external stimuli and signals in modulating cellulase production in T. reesei Rut-C30 remains unclear. Calcium is a known second messenger regulating cellulase gene expression in T. reesei . Results: In this study, we found that a biologically relevant extracellular Mn2+ concentration markedly stimulates cellulase production, total protein secretion, and the intracellular Mn2+ concentration of Rut-C30, a cellulase hyper-producing strain of T...
2018: Biotechnology for Biofuels
Yusuke Sone, Shuto Nakamura, Makoto Sasaki, Fumihito Hasebe, Seung-Young Kim, Nobutaka Funa
1,8-Dihydroxynaphthalene (1,8-DHN) is a key intermediate in the biosynthesis of DHN melanin, which is specific to fungi. In this study, we characterized the enzymatic properties of the gene products of an operon consisting of soceCHS1 , bdsA , and bdsB from the gram-negative bacterium, Sorangium cellulosum Heterologous expression of soceCHS1 , bdsA , and bdsB in Streptomyces coelicolor caused secretion of a dark-brown pigment into the broth. High-performance liquid chromatography analysis of the broth revealed that the recombinant strain produced 1,8-DHN, indicating that the operon encoded a novel enzymatic system for the synthesis of 1,8-DHN...
March 2, 2018: Applied and Environmental Microbiology
A E Naas, L M Solden, A D Norbeck, H Brewer, L H Hagen, I M Heggenes, A C McHardy, R I Mackie, L Paša-Tolić, M Ø Arntzen, V G H Eijsink, N M Koropatkin, M Hess, K C Wrighton, P B Pope
BACKGROUND: In nature, obligate herbivorous ruminants have a close symbiotic relationship with their gastrointestinal microbiome, which proficiently deconstructs plant biomass. Despite decades of research, lignocellulose degradation in the rumen has thus far been attributed to a limited number of culturable microorganisms. Here, we combine meta-omics and enzymology to identify and describe a novel Bacteroidetes family ("Candidatus MH11") composed entirely of uncultivated strains that are predominant in ruminants and only distantly related to previously characterized taxa...
March 1, 2018: Microbiome
Maíra de Freitas Pereira, Claire Veneault-Fourrey, Patrice Vion, Fréderic Guinet, Emmanuelle Morin, Kerrie W Barry, Anna Lipzen, Vasanth Singan, Stephanie Pfister, Hyunsoo Na, Megan Kennedy, Simon Egli, Igor Grigoriev, Francis Martin, Annegret Kohler, Martina Peter
Cenococcum geophilum is an ectomycorrhizal fungus with global distribution in numerous habitats and associates with a large range of host species including gymnosperm and angiosperm trees. Moreover, C. geophilum is the unique ectomycorrhizal species within the clade Dothideomycetes, the largest class of Ascomycetes containing predominantly saprotrophic and many devastating phytopathogenic fungi. Recent studies highlight that mycorrhizal fungi, as pathogenic ones, use effectors in form of Small Secreted Proteins (SSPs) as molecular keys to promote symbiosis...
2018: Frontiers in Microbiology
Maryam Esmailbeig, Abbas Ghaderi
Interleukin (IL)-18, structurally similar to IL-1β, is a member of IL-1 superfamily of cytokines. This cytokine, which is expressed by many human lymphoid and nonlymphoid cells, has an important role in inflammatory processes. The main function of IL-18 is mediated through induction of interferon-γ (IFN-γ) secretion from T helper (Th1) cells. This cytokine synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi...
November 1, 2017: European Cytokine Network
Sawsan Amara, Thomas Perrot, David Navarro, Aurélie Deroy, Amine Benkhelfallah, Amani Chalak, Marianne Daou, Didier Chevret, Craig B Faulds, Jean Guy Berrin, Mélanie Morel-Rouhier, Eric Gelhaye, Eric Record
Trametes versicolor is a wood inhabiting Agaricomycete known for its ability to cause strong white rot decay on hardwood and for its high tolerance toward phenolic compounds. The goal of the present work was to give insights on the molecular biology and biochemistry of heme-including class-II and dye-decolorizing peroxidases secreted from this fungus. Proteomic analysis of the secretome of T. versicolor BRFM1218 grown on oak wood revealed a set of 200 secreted proteins among which were a dye-decolorizing peroxidase Tv DyP1 and a versatile peroxidase Tv VP2...
February 16, 2018: Applied and Environmental Microbiology
Sara Sopeña-Torres, Lucía Jordá, Clara Sánchez-Rodríguez, Eva Miedes, Viviana Escudero, Sanjay Swami, Gemma López, Mariola Piślewska-Bednarek, Ines Lassowskat, Justin Lee, Yangnan Gu, Sabine Haigis, Danny Alexander, Sivakumar Pattathil, Antonio Muñoz-Barrios, Pawel Bednarek, Shauna Somerville, Paul Schulze-Lefert, Michael G Hahn, Dierk Scheel, Antonio Molina
Mitogen-activated protein kinases (MAPKs) cascades play essential roles in plants by transducing developmental cues and environmental signals into cellular responses. Among the latter are microbe-associated molecular patterns perceived by pattern recognition receptors (PRRs), which trigger immunity. We found that YODA (YDA) - a MAPK kinase kinase regulating several Arabidopsis developmental processes, like stomatal patterning - also modulates immune responses. Resistance to pathogens is compromised in yda alleles, whereas plants expressing the constitutively active YDA (CA-YDA) protein show broad-spectrum resistance to fungi, bacteria, and oomycetes with different colonization modes...
February 16, 2018: New Phytologist
Anmoldeep Randhawa, Olusola A Ogunyewo, Danish Eqbal, Mayank Gupta, Syed Shams Yazdani
Background: There is an urgent requirement for second-generation bio-based industries for economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of Penicillium funiculosum NCIM1228 showed high commercial potential by exhibiting high biomass hydrolyzing efficiency. To develop NCIM1228 further as an industrial workhorse, one of the major genetic interventions needed is global deregulation of cellulolytic genes to achieve higher enzyme production...
2018: Biotechnology for Biofuels
Xujun Zhang, Stephanie M Kirby, Yuwu Chen, Shelley L Anna, Lynn M Walker, Francisco R Hung, Paul S Russo
Protein surfactants show great potential to stabilize foams, bubbles, and emulsions. An important family of surface active proteins, the hydrophobins, is secreted by filamentous fungi. Two hydrophobin classes have been recognized, with Class II exhibiting slightly better solubility than Class I, although neither is very soluble in water. Hydrophobins are small proteins (8-14 kDa), but they are larger and more rigid than typical surfactants such as sodium dodecyl sulfate. This rigidity seems to be manifested in the strength of adsorbed hydrophobin layers on oil droplets or air bubbles...
February 1, 2018: Colloids and Surfaces. B, Biointerfaces
Xing Wang, Baiwen Ma, Yaohui Bai, Huachun Lan, Huijuan Liu, Jiuhui Qu
Coagulation plays an important role in alleviating membrane fouling, and a noticeable problem is the development of microorganisms after long-time operation, which gradually secrete extracellular polymeric substances (EPS). To date, few studies have paid attention to the behavior of microorganisms in drinking water treatment with ultrafiltration (UF) membranes. Herein, the membrane biofouling was investigated with different aluminum and iron salts. We found that Al2(SO4)3·18H2O performed better in reducing membrane fouling due to the slower growth rate of microorganisms...
January 2018: Journal of Environmental Sciences (China)
Gabriel Schweizer, Karin Münch, Gertrud Mannhaupt, Jan Schirawski, Regine Kahmann, Julien Y Dutheil
Plants and fungi display a broad range of interactions in natural and agricultural ecosystems ranging from symbiosis to parasitism. These ecological interactions result in coevolution between genes belonging to different partners. A well-understood example is secreted fungal effector proteins and their host targets, which play an important role in pathogenic interactions. Biotrophic smut fungi (Basidiomycota) are well-suited to investigate the evolution of plant pathogens, because several reference genomes and genetic tools are available for these species...
February 1, 2018: Genome Biology and Evolution
Jenna E Foderaro, Lois M Douglas, James B Konopka
The fungal plasma membrane is critical for cell wall synthesis and other important processes including nutrient uptake, secretion, endocytosis, morphogenesis, and response to stress. To coordinate these diverse functions, the plasma membrane is organized into specialized compartments that vary in size, stability, and composition. One recently identified domain known as the Membrane Compartment of Can1 (MCC)/eisosome is distinctive in that it corresponds to a furrow-like invagination in the plasma membrane. MCC/eisosomes have been shown to be formed by the Bin/Amphiphysin/Rvs (BAR) domain proteins Lsp1 and Pil1 in a range of fungi...
November 3, 2017: Journal of Fungi (Basel, Switzerland)
Daniel Lanver, André N Müller, Petra Happel, Gabriel Schweizer, Fabian B Haas, Marek Franitza, Clément Pellegrin, Stefanie Reissmann, Janine Altmüller, Stefan A Rensing, Regine Kahmann
The corn smut fungus Ustilago maydis is a model organism for elucidating host colonization strategies of biotrophic fungi. Here we performed an in depth transcriptional profiling of the entire plant-associated development of U. maydis wild-type strains. In our analysis we focused on fungal metabolism, nutritional strategies, secreted effectors and regulatory networks. Secreted proteins were enriched in three distinct expression modules corresponding to stages on the plant surface, establishment of biotrophy and induction of tumors...
January 25, 2018: Plant Cell
Derry K Mercer, Colin S Stewart
Dermatophytes are the most common cause of superficial fungal infections (tinea infections) and are a specialized group of filamentous fungi capable of infecting and degrading keratinised tissues, including skin, hair, and nail. Essential to their pathogenicity and virulence is the production of a broad spectrum of proteolytic enzymes and other key proteins involved in keratin biodegradation and utilization of its breakdown products. The initial stage of biodegradation of native keratin is considered to be sulfitolysis, in which the extensive disulfide bridges present in keratin are hydrolyzed, although some secreted subtilisins can degrade dye-impregnated keratin azure without prior reduction (Sub3 and Sub4)...
January 17, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Guiqiu Zhao, Ming Hu, Cui Li, Jieun Lee, Kelan Yuan, Guoqiang Zhu, Chengye Che
Fungal keratitis is a major cause of corneal ulcers, resulting in significant visual impairment and blindness. A phosphorylated glycoprotein secreted by immunocompetent cells, osteopontin (OPN) mediates cluster formation of the host fungal receptors and enhances the phagocytosis and clearance of pathogenic fungi. However, whether OPN production and function occurs in fungal keratitis is unknown. OPN expression in Aspergillus fumigatus keratitis patient corneas was assessed by quantitative polymerase chain reaction (qRT-PCR) and immunofluorescence...
January 23, 2018: Immunology and Cell Biology
Eric C H Chen, Emmanuelle Morin, Denis Beaudet, Jessica Noel, Gokalp Yildirir, Steve Ndikumana, Philippe Charron, Camille St-Onge, John Giorgi, Manuela Krüger, Timea Marton, Jeanne Ropars, Igor V Grigoriev, Matthieu Hainaut, Bernard Henrissat, Christophe Roux, Francis Martin, Nicolas Corradi
Arbuscular mycorrhizal fungi (AMF) are known to improve plant fitness through the establishment of mycorrhizal symbioses. Genetic and phenotypic variations among closely related AMF isolates can significantly affect plant growth, but the genomic changes underlying this variability are unclear. To address this issue, we improved the genome assembly and gene annotation of the model strain Rhizophagus irregularis DAOM197198, and compared its gene content with five isolates of R. irregularis sampled in the same field...
January 22, 2018: New Phytologist
Runmao Lin, Feifei Qin, Baoming Shen, Qianqian Shi, Chichuan Liu, Xi Zhang, Yang Jiao, Jun Lu, Yaoyao Gao, Marta Suarez-Fernandez, Federico Lopez-Moya, Luis Vicente Lopez-Llorca, Gang Wang, Zhenchuan Mao, Jian Ling, Yuhong Yang, Xinyue Cheng, Bingyan Xie
Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (~44 Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (~41 Mb) isolated from cereal cyst nematode...
January 18, 2018: Scientific Reports
Eddy Patricia Lopez Molano, Odalys García Cabrera, Juliana Jose, Leandro Costa do Nascimento, Marcelo Falsarella Carazzolle, Paulo José Pereira Lima Teixeira, Javier Correa Alvarez, Ricardo Augusto Tiburcio, Paulo Massanari Tokimatu Filho, Gustavo Machado Alvares de Lima, Rafael Victório Carvalho Guido, Thamy Lívia Ribeiro Corrêa, Adriana Franco Paes Leme, Piotr Mieczkowski, Gonçalo Amarante Guimarães Pereira
BACKGROUND: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus...
January 17, 2018: BMC Genomics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"