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extracellular secreted protein

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https://www.readbyqxmd.com/read/28530703/trpv4-plays-a-role-in-breast-cancer-cell-migration-via-ca-2-dependent-activation-of-akt-and-downregulation-of-e-cadherin-cell-cortex-protein
#1
W H Lee, L Y Choong, T H Jin, N N Mon, S Chong, C S Liew, T Putti, S Y Lu, C Harteneck, Y P Lim
TRPV4 belongs to the 'Transient Receptor Potential' (TRP) superfamily. It has been identified to profoundly affect a variety of physiological processes, including nociception, heat sensation and inflammation. Unlike other TRP superfamily channels, its role in cancers are unknown until recently when we reported TRPV4 to be required for cancer cell softness that may promote breast cancer cell extravasation and metastasis. Here, we elucidated the molecular mechanisms mediated by TRPV4 in the metastatic breast cancer cells...
May 22, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28530169/commensal-derived-omvs-elicit-a-mild-proinflammatory-response-in-intestinal-epithelial-cells
#2
Daniel A Patten, Enas Hussein, Scott P Davies, Paul N Humphreys, Andrew Collett
Under normal physiological conditions, the intestinal immunity remains largely hyporesponsive to the commensal microbiota, yet also retains the inherent ability to rapidly respond to pathogenic antigens. However, immunomodulatory activities of extracellular products from commensal bacteria have been little studied, with previous investigations generally utilizing the live bacterium to study microbiota-epithelial interactions. In this study, we demonstrate that extracellular products of a commensal bacterium, Escherichia coli C25, elicit a moderate release of proinflammatory IL-8 and stimulate transcriptional up-regulation of Toll-like receptors (TLRs) in intestinal epithelial cell lines HT29-19A and Caco-2...
May 22, 2017: Microbiology
https://www.readbyqxmd.com/read/28529927/staphylococcus-aureus-manipulates-innate-immunity-through-own-and-host-expressed-proteases
#3
REVIEW
Giampiero Pietrocola, Giulia Nobile, Simonetta Rindi, Pietro Speziale
Neutrophils, complement system and skin collectively represent the main elements of the innate immune system, the first line of defense of the host against many common microorganisms. Bacterial pathogens have evolved strategies to counteract all these defense activities. Specifically, Staphylococcus aureus, a major human pathogen, secretes a variety of immune evasion molecules including proteases, which cleave components of the innate immune system or disrupt the integrity of extracellular matrix and intercellular connections of tissues...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#4
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
https://www.readbyqxmd.com/read/28526017/the-effect-of-hypoxia-on-the-lipidome-of-recombinant-pichia-pastoris
#5
Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum, Pau Ferrer
BACKGROUND: Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. In addition, transcriptomic analyses of hypoxic P. pastoris pointed out important regulation of lipid metabolism and unfolded protein response (UPR). Notably, UPR that plays a role in the regulation of lipid metabolism, amino acid metabolism and protein secretion, was found to be upregulated under hypoxia...
May 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28523947/potentiation-of-trap-6-induced-platelet-dense-granule-release-by-blockade-of-p2y12-signaling-with-mrs2395
#6
Annachiara Mitrugno, Rachel A Rigg, Nicole B Laschober, Anh T P Ngo, Jiaqing Pang, Craig D Williams, Joseph E Aslan, Owen J T McCarty
The release of ADP from platelet dense granules and its binding to platelet P2Y12 receptors is key to amplifying the initial hemostatic response and propagating thrombus formation. P2Y12 has thus emerged as a therapeutic target to safely and effectively prevent secondary thrombotic events in patients with acute coronary syndrome or a history of myocardial infarction. Pharmacological inhibition of P2Y12 receptors represents a useful approach to better understand the signaling mediated by these receptors and to elucidate the role of these receptors in a multitude of platelet hemostatic and thrombotic responses...
May 19, 2017: Platelets
https://www.readbyqxmd.com/read/28522569/brain-derived-neurotrophic-factor-and-airway-fibrosis-in-asthma
#7
Michelle R Freeman, Venkatachalem Sathish, Logan J Manlove, Shengyu Wang, Rodney D Britt, Michael A Thompson, Christina M Pabelick, Y S Prakash
Airway remodeling in asthma driven by inflammation involves proliferation of epithelial cells and airway smooth muscle (ASM), as well as enhanced extracellular matrix (ECM) generation and deposition, i.e. fibrosis. Accordingly, understanding pro-fibrotic mechanisms is important for developing novel therapeutic strategies in asthma. Recent studies, including our own, have suggested a role for locally-produced growth factors such as brain-derived neurotrophic factor (BDNF) in mediating and modulating inflammation effects...
May 18, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28521806/high-density-lipoprotein-promoting-proliferation-and-migration-of-type-ii-alveolar-epithelial-cells-during-inflammation-state
#8
Zhongji Yu, Jingru Jin, Yuhui Wang, Jian Sun
BACKGROUND: To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. METHODS: The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK)...
May 18, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28521391/role-of-stem-cell-derived-exosomes-in-cancer
#9
Junyi Wu, Zhen Qu, Zi-Wei Fei, Jun-Hua Wu, Chun-Ping Jiang
Exosomes are small, extracellular membrane- enclosed vesicles that contain a variety of molecules, including proteins, DNA, mRNA and non-coding RNA; these vesicles have been defined as new tools for intercellular communication between cells. Numerous types of cells, including stem cells, secrete exosomes into the extracellular environment, and are significant communicators in the tumor microenvironment. Stem cells are a unique cell population defined by their ability to indefinitely self-renew, differentiate into a variety of cell lines, and form clonal cell populations...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28518125/glycoproteomics-of-the-extracellular-matrix-a-method-for-intact-glycopeptide-analysis-using-mass-spectrometry
#10
Javier Barallobre-Barreiro, Ferheen Baig, Marika Fava, Xiaoke Yin, Manuel Mayr
Fibrosis is a hallmark of many cardiovascular diseases and is associated with the exacerbated secretion and deposition of the extracellular matrix (ECM). Using proteomics, we have previously identified more than 150 ECM and ECM-associated proteins in cardiovascular tissues. Notably, many ECM proteins are glycosylated. This post-translational modification affects protein folding, solubility, binding, and degradation. We have developed a sequential extraction and enrichment method for ECM proteins that is compatible with the subsequent liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of intact glycopeptides...
April 21, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28517970/osteoblast-progenitors-enhance-osteogenic-differentiation-of-periodontal-ligament-stem-cells
#11
Miao Yu, Limei Wang, Pengfei Ba, Linxia Li, Long Sun, Xiaoqi Duan, Pishan Yang, Chengzhe Yang, Qinfeng Sun
BACKGROUND: Osteoblasts and periodontal ligament stem cells (PDLSCs) play an important role in maintaining physiological function of periodontal tissues and participating in periodontal regeneration. Elucidation of interactions between osteoblasts and PDLSCs will aid understanding of periodontal regeneration mechanisms. This study aimed to determine whether pre-osteoblasts can promote osteoblastic/cementoblastic differentiation of PDLSCs. METHODS: PDLSCs were cultured alone (control group), or co-cultured indirectly with human gingival fibroblasts (HGFs) (HGFs group) or MC3T3-E1 cells (OB groups)...
May 18, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28512097/glutathionylation-of-yersinia-pestis-lcrv-and-its-effects-on-plague-pathogenesis
#12
Anthony Mitchell, Christina Tam, Derek Elli, Thomas Charlton, Patrick Osei-Owusu, Farbod Fazlollahi, Kym F Faull, Olaf Schneewind
Glutathionylation, the formation of reversible mixed disulfides between glutathione and protein cysteine residues, is a posttranslational modification previously observed for intracellular proteins of bacteria. Here we show that Yersinia pestis LcrV, a secreted protein capping the type III secretion machine, is glutathionylated at Cys(273) and that this modification promotes association with host ribosomal protein S3 (RPS3), moderates Y. pestis type III effector transport and killing of macrophages, and enhances bubonic plague pathogenesis in mice and rats...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28507985/control-of-neuronal-migration-and-aggregation-by-reelin-signaling-in-the-developing-cerebral-cortex
#13
REVIEW
Yuki Hirota, Kazunori Nakajima
The mammalian cerebral neocortex has a well-organized laminar structure, achieved by the highly coordinated control of neuronal migration. During cortical development, excitatory neurons born near the lateral ventricle migrate radially to reach their final positions to form the cortical plate. During this process, dynamic changes are observed in the morphologies and migration modes, including multipolar migration, locomotion, and terminal translocation, of the newborn neurons. Disruption of these migration processes can result in neuronal disorders such as lissencephaly and periventricular heterotopia...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28507504/effects-of-%C3%AE-2a-adrenoceptors-on-norepinephrine-secretion-from-the-locus-coeruleus-during-chronic-stress-induced-depression
#14
Bin Wang, Ying Wang, Qiong Wu, Hong-Ping Huang, Shao Li
Chronic stressors can often lead to the development of psychological disorders, such as depression and anxiety. The locus coeruleus (LC) is a stress sensitive brain region located in the pons, with noradrenergic neurons that project to the hypothalamus, especially the paraventricular nucleus (PVN) of the hypothalamus. The purpose of this paper is to better understand how alpha 2A-adrenoceptors (α2A-ARs) and LC-hypothalamus noradrenergic system participate in the pathophysiological mechanism of depression. In vivo norepinephrine (NE) release in the PVN triggered by electrical stimulation in the LC was detected with carbon fiber electrodes in depression model of rats induced by chronic unpredictable mild stress (CUMS)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28504115/protocatechuic-acid-inhibits-hepatitis-b-virus-replication-by-activating-erk1-2-pathway-and-down-regulating-hnf4%C3%AE-and-hnf1%C3%AE-in-vitro
#15
Xiao-Qing Dai, Wen-Tao Cai, Xiao Wu, Yong Chen, Feng-Mei Han
AIMS: Protocatechuic acid (PCA) is a phenolic compound found in many antiviral Chinese herbal medicines. HNF4α and HNF1α, the members of hepatocyte nuclear factor (HNF) family, play an important regulatory role in the gene transcription of hepatitis B virus (HBV). Previous studies found that PCA inhibited HBV antigen secretion and HBV DNA replication in HepG2.2.15 cells, but its anti-HBV mechanism has not been fully understood. We aim to illustrate the anti-HBV mechanism of PCA. MATERIALS AND METHODS: MTT was used to estimate cytotoxicity...
May 12, 2017: Life Sciences
https://www.readbyqxmd.com/read/28501017/golgi-independent-routes-support-protein-disulfide-isomerase-externalization-in-vascular-smooth-muscle-cells
#16
Thaís L S Araujo, Carolina G Fernandes, Francisco R M Laurindo
Extracellular pools of intracellular molecular chaperones are increasingly evident. The peri/epicellular(pec) pool of the endoplasmic reticulum redox chaperone protein disulfide isomerase-A1(PDI) is involved in thrombosis and vascular remodeling, while PDI externalization routes remain elusive. In endothelial cells, vesicular-type PDI secretion involves classical and unconventional pathways, while in platelets PDI exocytosis involves actin cytoskeleton. However, little is known about pecPDI in vascular smooth muscle cells(VSMC)...
May 3, 2017: Redox Biology
https://www.readbyqxmd.com/read/28500395/the-human-serum-protein-c4b-binding-protein-inhibits-pancreatic-iapp-induced-inflammasome-activation
#17
Klaudia Kulak, Gunilla T Westermark, Nikolina Papac-Milicevic, Erik Renström, Anna M Blom, Ben C King
AIMS/HYPOTHESIS: Inflammasome activation and subsequent IL-1β production is a driver of islet pathology in type 2 diabetes. Oligomers, but not mature amyloid fibrils, of human islet amyloid polypeptide (IAPP), which is co-secreted with insulin, trigger NOD-like receptor pyrin domain containing-3 (NLRP3) inflammasome activation. C4b-binding protein (C4BP), present in serum, binds to IAPP and affects transition of IAPP monomers and oligomers to amyloid fibrils. We therefore hypothesised that C4BP inhibits IAPP-mediated inflammasome activation and IL-1β production...
May 12, 2017: Diabetologia
https://www.readbyqxmd.com/read/28498801/extracellular-truncated-tau-causes-early-presynaptic-dysfunction-associated-with-alzheimer-s-disease-and-other-tauopathies
#18
Fulvio Florenzano, Corsetti Veronica, Gabriele Ciasca, Maria Teresa Ciotti, Anna Pittaluga, Gunedalina Olivero, Marco Feligioni, Filomena Iannuzzi, Valentina Latina, Michele Francesco Maria Sciacca, Alessandro Sinopoli, Danilo Milardi, Giuseppe Pappalardo, Marco De Spirito, Massimiliano Papi, Anna Atlante, Antonella Bobba, Antonella Borreca, Pietro Calissano, Giuseppina Amadoro
The largest part of tau secreted from AD nerve terminals and released in cerebral spinal fluid (CSF) is C-terminally truncated, soluble and unaggregated supporting potential extracellular role(s) of NH2-derived fragments of protein on synaptic dysfunction underlying neurodegenerative tauopathies, including Alzheimer's disease (AD). Here we show that sub-toxic doses of extracellular-applied human NH2tau 26-44 (aka NH2htau) -which is the minimal active moiety of neurotoxic 20-22kDa peptide accumulating in vivo at AD synapses and secreted into parenchyma- acutely provokes presynaptic deficit in K+-evoked glutamate release on hippocampal synaptosomes along with alteration in local Ca2+ dynamics...
April 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28498635/snx10-plays-a-critical-role-in-mmp9-secretion-via-jnk-p38-erk-signaling-pathway
#19
Chun Zhou, Ying Wang, Jin Peng, Cuixian Li, Peiqing Liu, Xiaoyan Shen
Matrix metalloproteinases (MMPs) plays a critical role in the degradation of extracellular matrix (ECM). Sorting nexin (SNX) 10 is a member of the SNX family, which functions in regulation of endosomal sorting and osteoclast activation, has been implicated to play an important role in the bone erosion of rheumatoid arthritis. In this study, we aimed to investigate the possible role of SNX10 on MMP9 secretion and the potential mechanism. By immunostaining and co-immunoprecipitation, we found that SNX10 was extensively co-localized with MMP9, indicating that SNX10 might participate in MMP9 trafficking...
May 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28498321/bdnf-binds-its-pro-peptide-with-high-affinity-and-the-common-val66met-polymorphism-attenuates-the-interaction
#20
Koichi Uegaki, Haruko Kumanogoh, Toshiyuki Mizui, Takatsugu Hirokawa, Yasuyuki Ishikawa, Masami Kojima
Most growth factors are initially synthesized as precursors then cleaved into bioactive mature domains and pro-domains, but the biological roles of pro-domains are poorly understood. In the present study, we investigated the pro-domain (or pro-peptide) of brain-derived neurotrophic factor (BDNF), which promotes neuronal survival, differentiation and synaptic plasticity. The BDNF pro-peptide is a post-processing product of the precursor BDNF. Using surface plasmon resonance and biochemical experiments, we first demonstrated that the BDNF pro-peptide binds to mature BDNF with high affinity, but not other neurotrophins...
May 12, 2017: International Journal of Molecular Sciences
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