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Oncology animal imaging

Kerstin Mueller, Rebecca Fahrig, Michael Manhart, Yu Deuerling-Zheng, Jarrett Rosenberg, Teri Moore, Arundhuti Ganguly, Nishita Kothary
RATIONALE AND OBJECTIVES: Intra-procedural measurement of hepatic perfusion following liver embolization continues to be a challenge. Blood volume imaging before and after interventional procedures would allow identifying the treatment end point or even allow predicting treatment outcome. Recent liver oncology studies showed the feasibility of parenchymal blood volume (PBV) imaging using an angiographic C-arm system. This study was done to evaluate the reproducibility of PBV measurements using cone beam computed tomography (CBCT) before and after embolization of the liver in a swine model...
October 10, 2016: Academic Radiology
Shadi A Esfahani, Pedram Heidari, Sun A Kim, Shuji Ogino, Umar Mahmood
Purpose: To assess optical imaging of Mesenchymal-Epithelial Transition factor (MET) for delineation and characterization of intrahepatic models of human hepatocellular carcinoma (HCC) and metastatic colorectal cancer (CRC), and thereby demonstrate its potential use in precision oncology. Materials and Methods: MET expression in human CRC and HCC was assessed in tissue microarrays. We used GE-137, a modified cyanine 5-tagged peptide for MET targeting. HepG2 and Huh-7 (HCC) and HT-29 (CRC) cells with MET overexpression, and LNCaP cells (negative control) with minimal MET expression were incubated with the probe...
2016: Theranostics
Bridget F Koontz, Frank Verhaegen, Dirk De Ruysscher
Animal modelling is essential to the study of radiobiology and the advancement of clinical radiation oncology by providing preclinical data. Mouse models in particular, have been highly utilized in the study of both tumour and normal tissue radiobiology because of their cost effectiveness and versatility. Technology has significantly advanced in preclinical radiation techniques to allow highly conformal image guided irradiation of small animals in an effort to mimic human treatment capabilities. However, the biological and physical limitations of animal modelling should be recognized and considered when interpreting preclinical radiotherapy studies...
September 9, 2016: British Journal of Radiology
Simone U Dalm, Ingrid L Bakker, Erik de Blois, Gabriela N Doeswijk, Mark W Konijnenberg, Francesca Orlandi, Donato Barbato, Mattia Tedesco, Theodosia Maina, Berthold A Nock, Marion de Jong
INTRODUCTION: Since overexpression of the gastrin releasing peptide receptor (GRPR) has been reported on various cancer types, e.g. prostate cancer and breast cancer, targeting this receptor with radioligands might have significant impact on staging and treatment of GRPR-expressing tumors. NeoBOMB1 is a novel DOTA-coupled GRPR antagonist with high affinity for GRPR and excellent in vivo stability. The purpose of this preclinical study was to further explore the use of NeoBOMB1 for theranostic application by determining the biodistribution of (68)Ga-NeoBOMB1 and (177)Lu-NeoBOMB1...
September 8, 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Andrei Chekkoury, Antonio Nunes, Jerome Gateau, Panagiotis Symvoulidis, Annette Feuchtinger, Nicolas Beziere, Saak V Ovsepian, Axel Walch, Vasilis Ntziachristos
Diversity of the design and alignment of illumination and ultrasonic transducers empower the fine scalability and versatility of optoacoustic imaging. In this study, we implement an innovative high-resolution optoacoustic mesoscopy for imaging the vasculature and tissue oxygenation within subcutaneous and orthotopic cancerous implants of mice in vivo through acquisition of tomographic projections over 180° at a central frequency of 24 MHz. High-resolution volumetric imaging was combined with multispectral functional measurements to resolve the exquisite inner structure and vascularization of the entire tumor mass using endogenous and exogenous optoacoustic contrast...
August 2016: Neoplasia: An International Journal for Oncology Research
Jingfeng Luo, Xiaotian Wu, Fei Zhou, Yurong Zhou, Tongchun Huang, Fei Liu, Guocan Han, Luming Chen, Weixian Bai, Xia Wu, Jihong Sun, Xiaoming Yang
OBJECTIVE: Gene therapy is a frontier in modern medicine. In the present study, we explored a new technique for the effective treatment of multidrug-resistant (MDR) breast cancer by combining fully the advantages of multidisciplinary fields, including image-guided minimally invasive interventional oncology, radiofrequency technology, and direct intratumoral gene therapy. RESULTS: Combination treatment with PHSP-TK plus RFH resulted in significantly higher TK gene transfection/expression, as well as a lower cell proliferation rate and a higher cell apoptosis index, than those of control groups...
August 17, 2016: Oncotarget
Qi Li, Yajie Wang, Hongbin Xiao, Yujie Li, Xiaoxi Kan, Xiaomin Wang, Ganlin Zhang, Zhixin Wang, Qing Yang, Xi Chen, Xiaogang Weng, Ying Chen, Bingbing Zhou, Yan Guo, Xucen Liu, Xiaoxin Zhu
Metastasis is the leading lethal factor severely restraining the effectiveness of clinical treatment. TGF-beta is the key regulator for metastasis and influences paradoxically on cancer progression. The known TGF-beta blockers exert little selectivity on its functions, indiscriminately causing the anti-metastatic and pro-growth effects. Under such circumstances, specifically rebalancing the oncological function of TGF-beta provides a crucial oncotarget against metastasis. In our study, we established the screening platform targeting cell motility and identified a potential flavonoid, Chamaejasmenin B (ICJ), extracted from Stellera chamaejasme L...
June 21, 2016: Oncotarget
Sara Rapic, Christel Vangestel, Jeroen Verhaeghe, David Thomae, Patrick Pauwels, Tim Van den Wyngaert, Steven Staelens, Sigrid Stroobants
PURPOSE: In oncology, positron emission tomography imaging using dedicated tracers as biomarkers may assist in early evaluation of therapy efficacy. Using 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT), we investigated the early effects of chemotherapeutic treatment on cancer cell proliferation in a BRAF-mutated colorectal cancer xenograft model. PROCEDURES: Colo205 subcutaneously inoculated animals underwent 90-min dynamic imaging before and 24 h after treatment with vehicle (control), cetuximab (resistant) or irinotecan (sensitive)...
June 20, 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Eva M Serrao, Tiago B Rodrigues, Ferdia A Gallagher, Mikko I Kettunen, Brett W C Kennedy, Sarah L Vowler, Keith A Burling, Kevin M Brindle
Imaging of the metabolism of hyperpolarized [1-(13) C]pyruvate has shown considerable promise in preclinical studies in oncology, particularly for the assessment of early treatment response. The repeatability of measurements of (13) C label exchange between pyruvate and lactate was determined in a murine lymphoma model in fasted and non-fasted animals. The fasted state showed lower intra-individual variability, although the [1-(13) C]lactate/[1-(13) C]pyruvate signal ratio was significantly greater in fasted than in non-fasted mice, which may be explained by the higher tumor lactate concentrations in fasted animals...
August 2016: NMR in Biomedicine
Owen Peng, Walter J Akers
Optical imaging of fluorescent reporters in animal models of cancer has become a common tool in oncologic research. Fluorescent reporters including fluorescent proteins, organic dyes, and inorganic photonic materials are used in fluorescence spectroscopy, microscopy, and whole body preclinical imaging. Fluorescence lifetime imaging provides additional, quantitative information beyond that of conventional fluorescence intensity signals, enabling signal multiplexing, background separation, and biological sensing unique to fluorescent materials...
2016: Methods in Molecular Biology
Debora Petroni, Antonietta Bartoli, Simona Rapposelli, Maria Digiacomo, Silvia Burchielli, Giulia Nesi, Annalina Lapucci, Silvia Pardini, Sabrina Fucci, Marco Macchia, Piero A Salvadori, Luca Menichetti
PURPOSE: The plasma membrane P-glycoprotein (Pgp) is an efflux transporter involved in multidrug resistance and in the onset of neurodegenerative disease. Its function and most mechanisms of action are still under investigation. We developed a C-11-labeled 2-arylethylphenylamine-([(11)C]AEPH) derivative for positron emission tomography (PET), as a novel probe to better understand the activity and the function of Pgp in vivo. PROCEDURES: The synthetic procedure and the quality control of the selected lead compound, [(11)C]AEPH-1, were set up and optimized...
May 27, 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Cristina Martelli, Alessia Lo Dico, Cecilia Diceglie, Giovanni Lucignani, Luisa Ottobrini
Cancer is a complex disease, characterized by alteration of different physiological molecular processes and cellular features. Keeping this in mind, the possibility of early identification and detection of specific tumor biomarkers by non-invasive approaches could improve early diagnosis and patient management.Different molecular imaging procedures provide powerful tools for detection and non-invasive characterization of oncological lesions. Clinical studies are mainly based on the use of computed tomography, nuclear-based imaging techniques and magnetic resonance imaging...
April 27, 2016: Oncotarget
J Schwenck, K Fuchs, S H L Eilenberger, A-M Rolle, S Castaneda Vega, W M Thaiss, F C Maier
This review addresses small animal optical imaging (OI) applications in diverse fields of basic research. In the past, OI has proven to be cost- and time-effective, allows real-time imaging as well as high-throughput analysis and does not imply the usage of ionizing radiation (with the exception of Cerenkov imaging applications). Therefore, this technique is widely spread - not only geographically, but also among very different fields of basic research - and is represented by a large body of publications. Originally used in oncology research, OI is nowadays emerging in further areas like inflammation and infectious disease as well as neurology...
2016: Nuklearmedizin. Nuclear Medicine
Falk Tillner, Prasad Thute, Steffen Löck, Antje Dietrich, Andriy Fursov, Robert Haase, Mathias Lukas, Bernd Rimarzig, Manfred Sobiella, Mechthild Krause, Michael Baumann, Rebecca Bütof, Wolfgang Enghardt
Preclinical in vivo studies using small animals are essential to develop new therapeutic options in radiation oncology. Of particular interest are orthotopic tumour models, which better reflect the clinical situation in terms of growth patterns and microenvironmental parameters of the tumour as well as the interplay of tumours with the surrounding normal tissues. Such orthotopic models increase the technical demands and the complexity of preclinical studies as local irradiation with therapeutically relevant doses requires image-guided target localisation and accurate beam application...
April 21, 2016: Physics in Medicine and Biology
Philip J O'Halloran, Thomas Viel, David W Murray, Lydia Wachsmuth, Katrin Schwegmann, Stefan Wagner, Klaus Kopka, Monika A Jarzabek, Patrick Dicker, Sven Hermann, Cornelius Faber, Tim Klasen, Michael Schäfers, David O'Brien, Jochen H M Prehn, Andreas H Jacobs, Annette T Byrne
PURPOSE: Resistance to bevacizumab (BEV) in glioblastoma is believed to occur via activation of molecular networks including the mTOR/PI3K pathway. Using an MR/PET molecular imaging biomarker approach, we investigated the response to combining BEV with the mTOR/PI3K inhibitor BEZ235. METHODS: Tumours were established by orthotopically implanting U87MG-luc2 cells in mice. Animals were treated with BEZ235 and/or BEV, and imaged using diffusion-weighted-MRI, T2-weighted and T2*-weighted before and after administration of superparamagnetic iron oxide contrast agent...
August 2016: European Journal of Nuclear Medicine and Molecular Imaging
Sunil Sharma, Ganesh Narayanasamy, Beata Przybyla, Jessica Webber, Marjan Boerma, Richard Clarkson, Eduardo G Moros, Peter M Corry, Robert J Griffin
We have developed a small animal conformal radiation therapy device that provides a degree of geometrical/anatomical targeting comparable to what is achievable in a commercial animal irradiator. small animal conformal radiation therapy device is capable of producing precise and accurate conformal delivery of radiation to target as well as for imaging small animals. The small animal conformal radiation therapy device uses an X-ray tube, a robotic animal position system, and a digital imager. The system is in a steel enclosure with adequate lead shielding following National Council on Radiation Protection and Measurements 49 guidelines and verified with Geiger-Mueller survey meter...
January 20, 2016: Technology in Cancer Research & Treatment
Philippe Lambin, Jaap Zindler, Ben G L Vanneste, Lien Van De Voorde, Daniëlle Eekers, Inge Compter, Kranthi Marella Panth, Jurgen Peerlings, Ruben T H M Larue, Timo M Deist, Arthur Jochems, Tim Lustberg, Johan van Soest, Evelyn E C de Jong, Aniek J G Even, Bart Reymen, Nicolle Rekers, Marike van Gisbergen, Erik Roelofs, Sara Carvalho, Ralph T H Leijenaar, Catharina M L Zegers, Maria Jacobs, Janita van Timmeren, Patricia Brouwers, Jonathan A Lal, Ludwig Dubois, Ala Yaromina, Evert Jan Van Limbergen, Maaike Berbee, Wouter van Elmpt, Cary Oberije, Bram Ramaekers, Andre Dekker, Liesbeth J Boersma, Frank Hoebers, Kim M Smits, Adriana J Berlanga, Sean Walsh
A paradigm shift from current population based medicine to personalized and participative medicine is underway. This transition is being supported by the development of clinical decision support systems based on prediction models of treatment outcome. In radiation oncology, these models 'learn' using advanced and innovative information technologies (ideally in a distributed fashion - please watch the animation: from all available/appropriate medical data (clinical, treatment, imaging, biological/genetic, etc...
January 14, 2016: Advanced Drug Delivery Reviews
Alfonso Mastropietro, Annette Tennstaedt, Andreas Beyrau, Nadine Henn, Mathias Hoehn, Giuseppe Baselli
Bioluminescence Imaging (BLI) is an important molecular imaging tool to assess complex biological processes in vivo. BLI is a sensitive technique, which is frequently used in small-animal preclinical research, mainly in oncology and neurology. Tracking of labeled cells is one of the major applications. However, BLI data analysis for the segmentation of up-taking regions and their quantification is not trivial and it is usually an operator-dependent activity. In this work, a proof of concept of an automatic method to analyze BL images is presented which is based on a multi-step approach...
2015: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Juan José Vaquero, Paul Kinahan
Positron emission tomography (PET) imaging is based on detecting two time-coincident high-energy photons from the emission of a positron-emitting radioisotope. The physics of the emission, and the detection of the coincident photons, give PET imaging unique capabilities for both very high sensitivity and accurate estimation of the in vivo concentration of the radiotracer. PET imaging has been widely adopted as an important clinical modality for oncological, cardiovascular, and neurological applications. PET imaging has also become an important tool in preclinical studies, particularly for investigating murine models of disease and other small-animal models...
2015: Annual Review of Biomedical Engineering
James M P Stewart, Steve Ansell, Patricia E Lindsay, David A Jaffray
Advances in precision microirradiators for small animal radiation oncology studies have provided the framework for novel translational radiobiological studies. Such systems target radiation fields at the scale required for small animal investigations, typically through a combination of on-board computed tomography image guidance and fixed, interchangeable collimators. Robust targeting accuracy of these radiation fields remains challenging, particularly at the millimetre scale field sizes achievable by the majority of microirradiators...
December 7, 2015: Physics in Medicine and Biology
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