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anjiang tan yongping huang

Hong-Lun Bi, Jun Xu, An-Jiang Tan, Yong-Ping Huang
Custom-designed nuclease technologies such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) system provide attractive genome editing tools for insect functional genetics. The targeted gene mutagenesis mediated by the CRISPR/Cas9 system has been achieved in several insect orders including Diptera, Lepidoptera and Coleoptera. However, little success has been reported in agricultural pests due to the lack of genomic information and embryonic microinjection techniques in these insect species...
June 2016: Insect Science
Baosheng Zeng, Shuai Zhan, Yueqiang Wang, Yuping Huang, Jun Xu, Qun Liu, Zhiqian Li, Yongping Huang, Anjiang Tan
The CRISPR/Cas9 system has been proven as a revolutionary genome engineering tool. In most cases, single guide RNA (sgRNA) targeting sites have been designed as GN19NGG or GGN18NGG, because of restriction of the initiation nucleotide for RNA Pol III promoters. Here, we demonstrate that the U6 promoter from a lepidopteran model insect, Bombyx mori, effectively expressed the sgRNA initiated with any nucleotide bases (adenine, thymine, guanine or cytosine), which further expands the CRISPR targeting space. A detailed expansion index in the genome was analysed when N20NGG was set as the CRISPR targeting site instead of GN19NGG, and revealed a significant increase of suitable targets, with the highest increase occurring on the Z sex chromosome...
May 2016: Insect Biochemistry and Molecular Biology
Zhongjie Zhang, Abu F M Aslam, Xiaojing Liu, Muwang Li, Yongping Huang, Anjiang Tan
Recently established, custom-designed nuclease technologies such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system provide attractive genome editing tools. Targeted gene mutagenesis using the CRISPR/Cas9 system has been achieved in several orders of insects. However, outside of studies on Drosophila melanogaster and the lepidopteron model insect Bombyx mori, little success has been reported, which is largely due to a lack of effective genetic manipulation tools that can be used in other insect orders...
August 2015: Journal of Insect Physiology
Zhiqian Li, Lang You, Baosheng Zeng, Lin Ling, Jun Xu, Xu Chen, Zhongjie Zhang, Subba Reddy Palli, Yongping Huang, Anjiang Tan
Metamorphosis in insects includes a series of programmed tissue histolysis and remolding processes that are controlled by two major classes of hormones, juvenile hormones and ecdysteroids. Precise pulses of ecdysteroids (the most active ecdysteroid is 20-hydroxyecdysone, 20E), are regulated by both biosynthesis and metabolism. In this study, we show that ecdysone oxidase (EO), a 20E inactivation enzyme, expresses predominantly in the midgut during the early pupal stage in the lepidopteran model insect, Bombyx mori...
June 22, 2015: Proceedings. Biological Sciences
Lin Ling, Xie Ge, Zhiqian Li, Baosheng Zeng, Jun Xu, Xu Chen, Peng Shang, Anthony A James, Yongping Huang, Anjiang Tan
MicroRNAs (miRNAs) are post-transcriptional regulators that target specific mRNAs for repression and thus play key roles in many biological processes, including insect wing morphogenesis. miR-2 is an invertebrate-specific miRNA family that has been predicted in the fruit fly, Drosophila melanogaster, to be involved in regulating the Notch signaling pathway. We show here that miR-2 plays a critical role in wing morphogenesis in the silkworm, Bombyx mori, a lepidopteran model insect. Transgenic over-expression of a miR-2 cluster using a Gal4/UAS system results in deformed adult wings, supporting the conclusion that miR-2 regulates functions essential for normal wing morphogenesis...
2015: RNA Biology
Zhiqian Li, Baosheng Zeng, Lin Ling, Jun Xu, Lang You, Abu F M Aslam, Anjiang Tan, Yongping Huang
RNA interference has been described as a powerful genetic tool for gene functional analysis and a promising approach for pest management. However, RNAi efficiency varies significantly among insect species due to distinct RNAi machineries. Lepidopteran insects include a large number of pests as well as model insects, such as the silkworm, Bombyx mori. However, only limited success of in vivo RNAi has been reported in lepidoptera, particularly during the larval stages when the worms feed the most and do the most harm to the host plant...
2015: International Journal of Biological Sciences
Yueqiang Wang, Anjiang Tan, Jun Xu, Zhiqian Li, Baosheng Zeng, Lin Ling, Lang You, Yazhou Chen, Anthony A James, Yongping Huang
Transposon-based genetic transformation has facilitated insect functional genomics and new strategies of pest management. However, there is a need for alternative, site-specific approaches to overcome limitations of random integration (and associated position-effects) and potential instability of inserted transgenes. Here we describe a transposon-free, site-specific genetic transformation system mediated by transcription activator-like effector nucleases (TALENs) in the silkworm, Bombyx mori, a lepidopteran model insect...
December 2014: Insect Biochemistry and Molecular Biology
Zhiqian Li, Xie Ge, Lin Ling, Baosheng Zeng, Jun Xu, Abu F M Aslam, Lang You, Subba Reddy Palli, Yongping Huang, Anjiang Tan
Insect development and metamorphosis are regulated by two major hormones, juvenile hormone and ecdysteroids. Despite being the key regulator of insect developmental transitions, the metabolic pathway of the primary steroid hormone, 20-hydroxyecdysone (20E), especially its inactivation pathway, is still not completely elucidated. A cytochrome P450 enzyme, CYP18A1, has been shown to play key roles in insect steroid hormone inactivation through 26-hydroxylation. Here, we identified two CYP18 (BmCYP18A1 and BmCYP18B1) orthologs in the lepidopteran model insect, Bombyx mori...
November 2014: Insect Biochemistry and Molecular Biology
Yazhou Chen, Muwang Li, Iftakher Islam, Lang You, Yueqiang Wang, Zhiqian Li, Lin Ling, Baosheng Zeng, Jun Xu, Yongping Huang, Anjiang Tan
Understanding the mechanism of Bt resistance is one of the key elements of the effective application of Bt in pest control. The lepidopteran model insect, the silkworm, demonstrates qualities that make it an ideal species to use in achieving this understanding. We screened 45 strains of silkworm (Bombyx mori) using a Cry1Ab toxin variant. The sensitivity levels of the strains varied over a wide range. A resistant strain (P50) and a phylogenetically related susceptible strain (Dazao) were selected to profile the expressions of 12 Bt resistance-related genes...
November 2014: Insect Biochemistry and Molecular Biology
Lin Ling, Xie Ge, Zhiqian Li, Baosheng Zeng, Jun Xu, Abu F M Aslam, Qisheng Song, Peng Shang, Yongping Huang, Anjiang Tan
MicroRNAs (miRNAs) are a class of endogenous, non-coding, regulatory RNA molecules that post-transcriptionally regulate gene expression by binding to the 3'UTRs of mRNA targets and thus cause their degradation or translational inhibition. In insects, important roles of miRNAs in various biological processes have been demonstrated in Drosophila melanogaster. However, biological roles of miRNAs are barely unveiled in the majority of insect species due to limited genetic tools. In the present study, we introduce the transgenic miRNA sponge (miR-SP) technology combining with the binary GAL4/UAS system in the domesticated silkworm, Bombyx mori, to exploit the biological function of an evolutionally conserved miRNA, let-7...
October 2014: Insect Biochemistry and Molecular Biology
Zhiqian Li, Jianhao Jiang, Yazhou Chen, Lang You, Yongping Huang, Anjiang Tan, Zhiqian Li, Jianhao Jiang, Baolong Niu, Zhiqi Meng
The PAR-domain protein 1 (PDP1) is essential for locomotor activity of insects. However, its functions in insect growth and development have not been studied extensively, which prompted our hypothesis that PDP1 acts in energy metabolism. Here we report identification of TcPDP1 in the red flour beetle, Tribolium castaneum, and its functional analysis by RNAi. Treating larvae with dsTcPDP1 induced pupae developmental arrestment, accompanied by accelerated fat body degradation. dsTcPDP1 treatments in adults resulted in reduced female fecundity...
March 2014: Archives of Insect Biochemistry and Physiology
Jianhao Jiang, Xie Ge, Zhiqian Li, Yueqiang Wang, Qisheng Song, David W Stanley, Anjiang Tan, Yongping Huang
Insect development and metamorphosis are regulated by the coordination of ecdysone and juvenile hormones. Insect microRNAs (miRNAs) also act in insect development and metamorphosis by regulating genes in the ecdysone cascade. Although hundreds of insect miRNAs have been identified, the physiological functions of most remain poorly understood. Here, we report that a conserved insect miRNA, microRNA-281 (miR-281), regulates the ecdysone receptor (EcR), in an isoform-specific manner in the silkworm Bombyx mori...
August 2013: Insect Biochemistry and Molecular Biology
Anjiang Tan, Guoliang Fu, Li Jin, Qiuhong Guo, Zhiqian Li, Baolong Niu, Zhiqi Meng, Neil I Morrison, Luke Alphey, Yongping Huang
Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only...
April 23, 2013: Proceedings of the National Academy of Sciences of the United States of America
Xie Ge, Yong Zhang, Jianhao Jiang, Yi Zhong, Xiaonan Yang, Zhiqian Li, Yongping Huang, Anjiang Tan
The current identification of microRNAs (miRNAs) in insects is largely dependent on genome sequences. However, the lack of available genome sequences inhibits the identification of miRNAs in various insect species. In this study, we used a miRNA database of the silkworm Bombyx mori as a reference to identify miRNAs in Helicoverpa armigera and Spodoptera litura using deep sequencing and homology analysis. Because all three species belong to the Lepidoptera, the experiment produced reliable results. Our study identified 97 and 91 conserved miRNAs in H...
2013: International Journal of Biological Sciences
Yunpo Zhao, Hao Zhang, Zhiqian Li, Jianli Duan, Jianhao Jiang, Yubing Wang, Shuai Zhan, Rotimi O Akinkurolere, Anying Xu, Heying Qian, Xuexia Miao, Anjiang Tan, Yongping Huang
MucK is a member of the major facilitator super family (MFS) and is within a subgroup involved in the uptake of organic acids. Here, we provide the first evidence that mucK is required for normal body color pattern in insect larvae. In the cts mutant strain of Bombyx mori (Linnaeus), the larval anal plates, prothoracic tergum, and the head are reddish brown, as opposed to the white color found in the wild type. Positional cloning of the cts gene was carried out and it was mapped within 90kb on the 16th chromosome...
November 2012: Journal of Insect Physiology
Dan Liu, Shanchun Yan, Yongping Huang, Anjiang Tan, David W Stanley, Qisheng Song
The Asian corn borer, Ostrinia furnacalis, is a serious pest of corn, sorghum, and cotton in China and other Asian countries. The present study is the first attempt to establish the transgenic line in O. furnacalis using a piggyBac transposon, which will shed light on the future genetic control of O. furnacalis. A piggyBac vector pBac[A3EGFP] was constructed to express enhanced green fluorescence protein (EGFP)under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of pBac[A3EGFP] and the excision assay showed the transgenic vector was precisely excised...
August 2012: Archives of Insect Biochemistry and Physiology
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