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Amniotic injection

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https://www.readbyqxmd.com/read/27895585/feto-maternal-trafficking-of-exosomes-in-murine-pregnancy-models
#1
Samantha Sheller-Miller, Jun Lei, George Saade, Carlos Salomon, Irina Burd, Ramkumar Menon
Timing and initiation of labor are well-orchestrated by signals communicated between the fetal and maternal compartments; however, how these signals are communicated is not completely understood. Fetal exosomes, intercellular signaling vesicles, may play a key role in the process. The objective of this study was to evaluate exosome trafficking in vivo from fetal to maternal compartments. Pregnant CD-1 mice were intra-amniotically injected on gestational day 16 and 17 with exosomes isolated from primary human amnion epithelial cells fluorescently labeled with the lipophilic dye 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR)...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27845396/first-steps-to-define-murine-amniotic-fluid-stem-cell-microenvironment
#2
E Bertin, M Piccoli, C Franzin, G Spiro, S Donà, A Dedja, F Schiavi, E Taschin, P Bonaldo, P Braghetta, P De Coppi, M Pozzobon
Stem cell niche refers to the microenvironment where stem cells reside in living organisms. Several elements define the niche and regulate stem cell characteristics, such as stromal support cells, gap junctions, soluble factors, extracellular matrix proteins, blood vessels and neural inputs. In the last years, different studies demonstrated the presence of cKit(+) cells in human and murine amniotic fluid, which have been defined as amniotic fluid stem (AFS) cells. Firstly, we characterized the murine cKit(+) cells present both in the amniotic fluid and in the amnion...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27844495/ileal-muc2-gene-expression-and-microbial-population-but-not-growth-performance-and-immune-response-are-influenced-by-in-ovo-injection-of-probiotics-in-broiler-chickens
#3
A Majidi-Mosleh, A A Sadeghi, S N Mousavi, M Chamani, A Zarei
1. The objective of present study was to evaluate the effects of intra-amniotic injection of different probiotic strains (Bacillus subtilis, Enterococcus faecium and Pediococcus acidilactici) on the intestinal MUC2 gene expression, microbial population, growth performance and immune response in broiler chicken. 2. In a completely randomised design, different probiotic strains was injected into the amniotic fluid of the 480 live embryos (d 18 of incubation), with 4 treatments and 5 replicates. Ileal MUC2 gene expression, microbial profile, growth performance and immune response were determined...
November 15, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27836365/transamniotic-stem-cell-therapy-trascet-in-a-leporine-model-of-gastroschisis
#4
Christina Feng, Christopher D Graham, Hester F Shieh, Joseph A Brazzo, John Patrick Connors, Lucas Rohrer, Alexander Papadakis, David Zurakowski, Dario O Fauza
BACKGROUND/PURPOSE: Transamniotic stem cell therapy (TRASCET) with amniotic fluid mesenchymal stem cells (afMSCs) has been shown to mitigate bowel damage in a rodent model of gastroschisis. As a prerequisite to clinical translation, we sought to study TRASCET in a larger animal model. METHODS: New Zealand rabbit fetuses (n=64) with surgically created gastroschisis were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intraamniotic injections of either saline or a suspension of afMSCs...
October 26, 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27821667/obeticholic-acid-protects-against-lipopolysaccharide-induced-fetal-death-and-intrauterine-growth-restriction-through-its-anti-inflammatory-activity
#5
Yuan-Hua Chen, Xiao-Guang Hu, Yan Zhou, Zhen Yu, Lin Fu, Gui-Bin Zhang, Qing-Li Bo, Hua Wang, Cheng Zhang, De-Xiang Xu
Farnesoid X receptor (FXR) is expressed in human and rodent placentas. Nevertheless, its function remains obscure. This study investigated the effects of obeticholic acid (OCA), a novel synthetic FXR agonist, on LPS-induced fetal death and intrauterine growth restriction. All pregnant mice except controls were i.p. injected with LPS (100 μg/kg) daily from gestational day (GD) 15 to GD17. Some pregnant mice were orally administered with OCA (5 mg/kg) daily from GD13 to GD17. As expected, placental FXR signaling was activated by OCA...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27789047/self-induced-illegal-abortion-with-rivanol-%C3%A2-a-medicolegal-toxicological-case-report
#6
Sarah C Koelzer, Hannelore Held, Stefan W Toennes, Marcel A Verhoff, Cora Wunder
Approximately during the 30th week of pregnancy, a woman gave birth to a still-born child in a hospital. After first citing an extraneous cause for the premature still-birth, the woman later admitted to having self-induced the abortion by injecting the antiseptic Rivanol(®) (active agent: ethacridine lactate) through her abdominal wall into the amniotic cavity. The investigating authorities ordered an autopsy of the fetus along with additional toxicological investigations. To the naked eye, no obvious cause of death was apparent...
August 27, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27774699/characterization-of-teratogenic-potential-and-gene-expression-in-canine-and-feline-amniotic-membrane-derived-stem-cells
#7
M T Cardoso, A O Pinheiro, A S Vidane, J B Casals, V C de Oliveira, Njn Gonçalves, D S Martins, C E Ambrósio
The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27774657/transplantation-of-amniotic-membrane-derived-multipotent-cells-ameliorates-and-delays-the-progression-of-chronic-kidney-disease-in-cats
#8
A S Vidane, A O Pinheiro, J B Casals, D Passarelli, McFns Hage, R S Bueno, D S Martins, C E Ambrósio
Chronic kidney disease (CKD) is a common clinical condition in domestic cats, characterized by tubulointerstitial, vascular and glomerular inflammation and severe fibrosis. Studies in rodent model of induced CKD have shown a decrease and stabilization of the clinical condition. In this study was evaluated the safety and effect of intrarenal and intravenous infusion of allogeneic mesenchymal stem cells (AMSCs) derived from feline amniotic membrane in cats with naturally occurring CKD. Cat AMSCs were harvested after mechanical and enzymatic digestion of amnion...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27741192/intra-fetal-compared-with-intra-amniotic-digoxin-before-dilation-and-evacuation-a-randomized-controlled-trial
#9
Katharine OʼConnell White, Deborah L Nucatola, Carolyn Westhoff
OBJECTIVE: To compare the effectiveness of 1.0 mg intra-fetal or intra-amniotic digoxin to achieve fetal asystole before second-trimester surgical pregnancy termination. METHODS: In a randomized trial, women received 1.0 mg transabdominal intra-fetal or intra-amniotic digoxin on the day of laminaria placement before dilation and evacuation between 20 and 24 weeks of gestation. The primary outcome was incidence of fetal asystole, documented immediately before dilation and evacuation...
November 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27707109/efficacy-of-particulate-amniotic-membrane-and-umbilical-cord-tissues-in-attenuating-cartilage-destruction-in-an-osteoarthritis-model
#10
Andrew L Raines, Mei-Shu Shih, Lorraine Chua, Chen-Wei Su, Scheffer C G Tseng, Julie O'Connell
Osteoarthritis (OA) is a progressive degenerative joint disease, and to date, no disease-modifying OA drug exists. Amniotic membrane and umbilical cord products have been used clinically in several diseases due to their anti-inflammatory and antiscarring properties. In the present study, we sought to evaluate whether a particulate amniotic membrane and umbilical cord (AM/UC) matrix could aid in attenuating disease progression. Lewis rats underwent medial meniscus transection (MMT) to induce OA. Two weeks after surgery, animals received intra-articular injections (50 μL) of either 50 or 100 μg/μL particulate AM/UC or saline control and were subsequently euthanized 1 or 4 weeks later...
November 18, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27667559/effects-of-an-early-intervention-using-human-amniotic-epithelial-cells-in-a-copd-rat-model
#11
Limei Geng, Zhiqiang Chen, Hong Ren, Xiaoyan Niu, Xiangyan Yu, Hongqian Yan
The study aimed to investigate the effect of an early intervention using human amniotic epithelial cell (hAEC) in a rat model of chronic obstructive pulmonary disease (COPD). Twenty-four specific pathogen-free Wistar rats were randomized to the control, COPD, and COPD+hAEC groups. COPD was established by intratracheal LPS injection combined with smoke fumigation over 30days. On the first day of model establishment rats in the AEC group also received intratracheal instillation of 500,000 hAECs isolated from the placenta of healthy donors...
November 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/27631175/study-of-transected-sciatic-nerve-repair-by-amniotic-membrane-with-betamethasone-in-adult-albino-wistar-rats
#12
Seyed Homayoon Sadraie, Kazem Parivar, Farzaneh Arabi, Mehrnaz Moattari, Gholamreza Kaka, Korosh Mansouri
BACKGROUND: The aim of this study was to determine the effects of amniotic membrane impregnated with betamethasone on regeneration of transected sciatic nerve injury in adult albino Wister rats. METHODS: In this research, 42 male adult rats were divided into six equal groups. 1) Normal (intact) group: healthy rats without any injury; 2) CONTROL GROUP: sciatic nerve was cut and sutured; 3) Sham group: 0.2 mL culture medium was injected on the epineurium in the injury; 4) Amniotic membrane group (AM): Acellular amniotic membrane was used around the damaged sciatic nerve; 5) Betamethasone group (B): 0...
September 2016: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/27621924/effects-of-acellular-amniotic-membrane-matrix-and-bone-marrow-derived-mesenchymal-stem-cells-in-improving-random-skin-flap-survival-in-rats
#13
Farzaneh Chehelcheraghi, Hossein Eimani, Seyed Homayoonsadraie, Giti Torkaman, Abdollah Amini, Hamid Alavi Majd, Hashem Shemshadi
BACKGROUND: The necrotic skin flap represents a great challenge in plastic and reconstructive surgery. In this study, we evaluated the effect of bioscaffolds, acellular amniotic membranes (AAMs), and bone marrow-derived mesenchymal stem cells (BM-MSCs) on random skin flap (RSF) survival in rats by applying a cell-free extracellular matrix scaffold as a supportive component for the growth and proliferation of BM-MSCs on RSFs. AAM matrix scaffolds were created by incubating AMs in ethylenediaminetetraacetic acid 0...
June 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/27602314/magnetic-resonance-imaging-of-human-derived-amniotic-membrane-stem-cells-using-pegylated-superparamagnetic-iron-oxide-nanoparticles
#14
Maryam Naseroleslami, Kazem Parivar, Samideh Khoei, Nahid Aboutaleb
OBJECTIVE: The label and detection of cells injected into target tissues is an area of focus for researchers. Iron oxide nanoparticles can be used to label cells as they have special characteristics. The purpose of this study is to examine the effects of iron oxide nanoparticles on human-derived amniotic membrane stem cell (hAMCs) survival and to investigate the magnetic properties of these nanoparticles with increased contrast in magnetic resonance imaging (MRI). MATERIALS AND METHODS: In this experimental study, we initially isolated mesenchymal stem cells from amniotic membranes and analyzed them by flow cytometry...
2016: Cell Journal
https://www.readbyqxmd.com/read/27601620/intra-amniotic-ureaplasma-parvum-induced-maternal-and-fetal-inflammation-and-immune-responses-in-rhesus-macaques
#15
Paranthaman Senthamaraikannan, Pietro Presicce, Cesar M Rueda, Gunlawadee Maneenil, Augusto F Schmidt, Lisa A Miller, Ken B Waites, Alan H Jobe, Suhas G Kallapur, Claire A Chougnet
BACKGROUND:  Although Ureaplasma species are the most common organisms associated with prematurity, their effects on the maternal and fetal immune system remain poorly characterized. METHODS:  Rhesus macaque dams at approximately 80% gestation were injected intra-amniotically with 10(7) colony-forming units of Ureaplasma parvum or saline (control). Fetuses were delivered surgically 3 or 7 days later. We performed comprehensive assessments of inflammation and immune effects in multiple fetal and maternal tissues...
November 15, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27588134/therapeutic-potential-of-human-amniotic-membrane-derived-mesenchymal-stem-cells-in-app-transgenic-mice
#16
Hongliang Jiao, Ke Shi, Weijie Zhang, Liang Yang, Lu Yang, Fangxia Guan, Bo Yang
Growing evidence indicates that the presence of extensive oxidative stress plays an essential role in the initiation and progression of Alzheimer's disease (AD). Amyloid-β (Aβ) aggregation is involved in the elevation of oxidative stress, contributing to mitochondrial dysfunction and lipid peroxidation. In the present study, human placenta amniotic membrane-derived mesenchymal stem cells (hAMMSCs) were intravenously injected into C57BL/6J-APP transgenic mice. hAMMSCs significantly ameliorated spatial learning and memory function, and were associated with a decreased amount of amyloid plaques of the brain...
September 2016: Oncology Letters
https://www.readbyqxmd.com/read/27585525/comparative-study-of-allogenic-and-xenogeneic-mesenchymal-stem-cells-on-cisplatin-induced-acute-kidney-injury-in-sprague-dawley-rats
#17
Rehab H Ashour, Mohamed-Ahdy Saad, Mohamed-Ahmed Sobh, Fatma Al-Husseiny, Mohamed Abouelkheir, Amal Awad, Doaa Elghannam, Hassan Abdel-Ghaffar, Mohamed Sobh
BACKGROUND: The paracrine and regenerative activities of mesenchymal stem cells (MSCs) may vary with different stem cell sources. The aim of the present study is to compare the effects of MSCs from different sources on acute kidney injury (AKI) induced by cisplatin and their influence on renal regeneration. METHODS: A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 120 Sprague-Dawley rats. Rats were treated with either rat bone marrow stem cells (rBMSCs), human adipose tissue-derived stem cells (hADSCs), or human amniotic fluid-derived stem cells (hAFSCs)...
September 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27583406/membrane-vesicles-of-group-b-streptococcus-disrupt-feto-maternal-barrier-leading-to-preterm-birth
#18
Manalee Vishnu Surve, Anjali Anil, Kshama Ganesh Kamath, Smita Bhutda, Lakshmi Kavitha Sthanam, Arpan Pradhan, Rohit Srivastava, Bhakti Basu, Suryendu Dutta, Shamik Sen, Deepak Modi, Anirban Banerjee
Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated with premature rupture of amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces membrane vesicles (MVs) in a serotype independent manner. These MVs are loaded with virulence factors including extracellular matrix degrading proteases and pore forming toxins. Mice chorio-decidual membranes challenged with MVs ex vivo resulted in extensive collagen degradation leading to loss of stiffness and mechanical weakening...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27548820/vascular-endothelial-growth-factor-up-regulation-in-human-amniotic-fluid-stem-cell-enhances-nephroprotection-after-ischemia-reperfusion-injury-in-the-rat
#19
Marina Gabriela Monteiro Carvalho Mori da Cunha, Silvia Zia, Diego Vilibaldo Beckmann, Marianne Sylvia Carlon, Fanny Oliveira Arcolino, Maarten Albersen, Ney Luis Pippi, Dominguita Lühers Graça, Conny Gysemans, Peter Carmeliet, Elena Levtchenko, Jan Deprest, Jaan Toelen
OBJECTIVE: To evaluate if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. DESIGN: Randomized animal study. SETTINGS: University research laboratory. SUBJECTS: A total of 40 males 12-week-old Wistar rats were subjected to ischemia-reperfusion and assigned to four groups: amniotic fluid stem cells, vascular endothelial growth factor-amniotic fluid stem cells in two different doses, and vehicle...
August 19, 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27514011/mesenchymal-stem-cells-in-corneal-neovascularization-comparison-of-different-application-routes
#20
Emma Ghazaryan, Yan Zhang, Yuxi He, Xin Liu, Ying Li, Jianan Xie, Guanfang Su
The purpose of the present study is to investigate the effect of mesenchymal stem cells in corneal neovascularization and wound healing, and to compare the effectiveness of two possible application routes, subconjunctival injection and amniotic membrane transplantation. Chemical injury was induced by application of sodium hydroxide to the rats' corneas. After 7 days, the animals were divided into three groups. Different treatment methods were used for each group as follows: i) Group 1, injection of bone marrow‑derived mesenchymal stem cells (BMSCs) under the conjunctiva; ii) group 2, transplantation of amniotic membranes, previously seeded with BMSCs; and iii) group 3, the untreated control group...
October 2016: Molecular Medicine Reports
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