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Amniotic injection

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https://www.readbyqxmd.com/read/29760719/ifn-%C3%AE-expressing-amniotic-fluid-derived-mesenchymal-stem-cells-migrate-to-and-suppress-hela-cell-derived-tumors-in-a-mouse-model
#1
Jun Zhou, Tian Liang, Dejun Wang, Liru Li, Yan Cheng, Qiuyan Guo, Guangmei Zhang
Background: Immunotherapy for cervical cancer with type I interferon (IFN) is limited because of the cytotoxicity that accompanies the high doses that are administered. In this study, we investigated the utilization of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) as a means for delivering IFN α to local tumor sites for the suppression of cervical cancer in a mouse model using HeLa cell xenografts. Methods: The tumor tropism ability of AF-MSCs and AF-MSCs genetically modified to overexpress IFN α (IFN α -AF-MSCs) was examined through Transwell in vitro and through fluorescent images and immunohistochemistry in a mouse model...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29718716/complex-chromosomal-aberrations-in-a-fetus-originating-from-oocytes-with-smooth-endoplasmic-reticulum-ser-aggregates
#2
Ioannis A Sfontouris, George T Lainas, Tryfon G Lainas, Efthimios Faros, Maria Banti, Katerina Kardara, Katerina Anagnostopoulou, Harris Kontos, George K Petsas, Efstratios M Kolibianakis
The presence of smooth endoplasmic reticulum aggregates (SERa) in the ooplasm is considered as the most severe oocyte dysmorphism due to its serious and potentially lethal outcomes in offspring. In the present case report, a couple underwent their first intracytoplasmic sperm injection (ICSI) cycle using a gonadotrophin releasing hormone (GnRH) antagonist protocol, followed by fetal ultrasound scanning and amniocentesis. SERa were observed in all oocytes retrieved. A singleton pregnancy was established. The second trimester fetal ultrasound scan revealed a female fetus with overlapping fingers in both hands, and amniocentesis was performed for the detection of chromosomal abnormalities...
May 2, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29715698/antenatal-vitamin-d-preserves-placental-vascular-and-fetal-growth-in-experimental-chorioamnionitis-due-to-intra-amniotic-endotoxin-exposure
#3
Michael W Cookson, Sharon L Ryan, Gregory J Seedorf, R Blair Dodson, Steve H Abman, Erica W Mandell
BACKGROUND:  Chorioamnionitis (CA) is associated with a high risk for the development of bronchopulmonary dysplasia (BPD) after preterm birth, but mechanisms that increase susceptibility for BPD and strategies to prevent BPD are uncertain. As a model of CA, antenatal intra-amniotic (IA) endotoxin (ETX) exposure alters placental structure, causes fetal growth restriction, increases perinatal mortality, and causes sustained cardiorespiratory abnormalities throughout infancy. Vitamin D (Vit D) has been shown to have both anti-inflammatory and proangiogenic properties...
May 1, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29706928/repetitive-neonatal-erythropoietin-and-melatonin-combinatorial-treatment-provides-sustained-repair-of-functional-deficits-in-a-rat-model-of-cerebral-palsy
#4
Lauren L Jantzie, Akosua Y Oppong, Fatu S Conteh, Tracylyn R Yellowhair, Joshua Kim, Gabrielle Fink, Adam R Wolin, Frances J Northington, Shenandoah Robinson
Cerebral palsy (CP) is the leading cause of motor impairment for children worldwide and results from perinatal brain injury (PBI). To test novel therapeutics to mitigate deficits from PBI, we developed a rat model of extreme preterm birth (<28 weeks of gestation) that mimics dual intrauterine injury from placental underperfusion and chorioamnionitis. We hypothesized that a sustained postnatal treatment regimen that combines the endogenous neuroreparative agents erythropoietin (EPO) and melatonin (MLT) would mitigate molecular, sensorimotor, and cognitive abnormalities in adults rats following prenatal injury...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29673373/chorioamnionitis-neuroinflammation-and-injury-timing-is-key-in-the-preterm-ovine-fetus
#5
Ruth Gussenhoven, Rob J J Westerlaken, Daan R M G Ophelders, Alan H Jobe, Matthew W Kemp, Suhas G Kallapur, Luc J Zimmermann, Per T Sangild, Stanislava Pankratova, Pierre Gressens, Boris W Kramer, Bobbi Fleiss, Tim G A M Wolfs
BACKGROUND: Antenatal infection (i.e., chorioamnionitis) is an important risk factor for adverse neurodevelopmental outcomes after preterm birth. Destructive and developmental disturbances of the white matter are hallmarks of preterm brain injury. Understanding the temporal effects of antenatal infection in relation to the onset of neurological injury is crucial for the development of neurotherapeutics for preterm infants. However, these dynamics remain unstudied. METHODS: Time-mated ewes were intra-amniotically injected with lipopolysaccharide at 5, 12, or 24 h or 2, 4, 8, or 15 days before preterm delivery at 125 days gestational age (term ~ 150 days)...
April 19, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29673009/in-utero-spontaneous-bladder-rupture-in-a-fetus-with-posterior-urethral-valve-a-case-report-of-prenatal-diagnosis-and-management
#6
Shoichi Magawa, Hiroaki Tanaka, Masafumi Nii, Shintaro Maki, Yuki Kamimoto, Tomoaki Ikeda
Posterior urethral valve (PUV) rarely causes bladder rupture. We experienced hydronephrosis due to ureteral obstruction after the natural repair of a ruptured bladder in a fetus with PUV. Fetal ascites and oligohydramnios were diagnosed at 26 weeks' gestational age. While we followed up with ultrasonography, we regularly removed the fetal ascites via abdominal puncture, injecting warm saline instead of amniotic fluid. At 35 weeks' gestational age, the infant was diagnosed with severe bilateral hydronephrosis, absent of ascites and oligohydramnios...
April 19, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29643795/ocular-injury-due-to-potassium-permanganate-granules
#7
Chareenun Chirapapaisan, Pinnita Prabhasawat, Sabong Srivannaboon, Vatookarn Roongpoovapatr, Piyada Chitsuthipakorn
Purpose: We report a rare case of ocular injury due to potassium permanganate (KMnO4 ) granules in a child. Methods: This is a retrospective case report. Results: A 2-year-old boy was transferred to our emergency room with severe pain in his right eye, inflamed eyelids, and brownish stains on his fingers. Chemical injury was suspected. Copious eye irrigation was immediately performed. Diffuse brownish splotches were then observed at the inferior bulbar conjunctiva...
January 2018: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/29580785/donor-mesenchymal-stem-cell-linetics-after-transamniotic-stem-cell-therapy-trascet-for-experimental-spina-bifida
#8
Hester F Shieh, Azra Ahmed, Lucas Rohrer, David Zurakowski, Dario O Fauza
PURPOSE: We sought to examine donor mesenchymal stem cell (MSC) kinetics after transamniotic stem cell therapy (TRASCET) in experimental spina bifida. METHODS: Pregnant Sprague-Dawley dams exposed to retinoic acid for the induction of fetal neural tube defects received volume-matched intra-amniotic injections on gestational day 17 (E17; term=E22): either amniotic fluid MSCs (afMSCs) labeled with a luciferase reporter gene (n=78), or luciferase protein alone (n=66)...
March 6, 2018: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29565705/zygomatic-dental-implant-induced-orbital-fracture-and-inferior-oblique-trauma
#9
Ann Q Tran, Daniela P Reyes-Capó, Nimesh A Patel, Joshua Pasol, Hilda Capó, Sara T Wester
A 51-year-old female underwent four upper zygomatic dental implants (ZI) and one upper and four lower conventional implants. Immediately postoperatively, the patient had pain and diplopia upon manual elevation of the edematous eyelid. Panoramic x-ray showed a malpositioned right upper ZI, requiring removal of the right upper ZI the following day. The patient had delayed referral to ophthalmology one month later for persistent diplopia. Computed tomography scan and magnetic resonance imaging demonstrated a right inferolateral fracture with fibrosis surrounding the inferior oblique muscle...
March 22, 2018: Orbit
https://www.readbyqxmd.com/read/29563340/il-1-signaling-mediates-intrauterine-inflammation-and-chorio-decidua-neutrophil-recruitment-and-activation
#10
Pietro Presicce, Chan-Wook Park, Paranthaman Senthamaraikannan, Sandip Bhattacharyya, Courtney Jackson, Fansheng Kong, Cesar M Rueda, Emily DeFranco, Lisa A Miller, David A Hildeman, Nathan Salomonis, Claire A Chougnet, Alan H Jobe, Suhas G Kallapur
Neutrophil infiltration of the chorioamnion-decidua tissue at the maternal-fetal interface (chorioamnionitis) is a leading cause of prematurity, fetal inflammation, and perinatal mortality. We induced chorioamnionitis in preterm rhesus macaques by intraamniotic injection of LPS. Here, we show that, during chorioamnionitis, the amnion upregulated phospho-IRAK1-expressed neutrophil chemoattractants CXCL8 and CSF3 in an IL-1-dependent manner. IL-1R blockade decreased chorio-decidua neutrophil accumulation, neutrophil activation, and IL-6 and prostaglandin E2 concentrations in the amniotic fluid...
March 22, 2018: JCI Insight
https://www.readbyqxmd.com/read/29537335/conservative-management-of-interstitial-pregnancies-experience-of-a-single-centre
#11
Semih Zeki Uludag, Mehmet Serdar Kutuk, Mehmet Dolanbay, Mahmut Tuncay Ozgun, Gunel Eliyeva, Ozguc Altun
The aim of this study was to compare the effect of local intracavitary methotrexate (MTX) injection and laparoscopic (L/S) cornuostomy in the treatment of interstitial pregnancy (IP) in terms of clinical and reproductive outcome. The data of patients with IP (n:10) who were treated between September 2011 and December 2016 with either an intra-amniotic MTX injection (n:7) or L/S cornuostomy (n:3) were retrospectively evaluated. All cases (7/7) in the local injection group and two (2/3) in the L/S group were successfully treated with the initial treatment...
March 14, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29523193/human-amniotic-mesenchymal-stem-cells-improve-ovarian-function-in-natural-aging-through-secreting-hepatocyte-growth-factor-and-epidermal-growth-factor
#12
Chenyue Ding, Qinyan Zou, Fuxin Wang, Huihua Wu, Rulei Chen, Jinghuan Lv, Mingfa Ling, Jian Sun, Wei Wang, Hong Li, Boxian Huang
BACKGROUND: Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies have looked at stem cell treatment of natural ovarian aging (NOA). We designed this experimental study to investigate whether human amniotic mesenchymal stem cells (hAMSCs) retain the ability to restore ovarian function, and how hAMSCs work in this process. METHODS: To build the NOA mouse model, the mice were fed for 12-14 months normally with young fertile female mice as the normal control group (3-5 months old)...
March 9, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29506937/dual-chemotactic-factors-secreting-human-amniotic-mesenchymal-stem-cells-via-talen-mediated-gene-editing-enhanced-angiogenesis
#13
In Sil Jeong, Youngjin Park, Hyun Aae Ryu, Hyun Sook An, Ju Hye Han, Sung-Whan Kim
BACKGROUND: Even though mesenchymal stem cells (MSCs) have angiogenic property, their cytokine secretory capacity is limited to treat ischemic vascular disorders. In present study, we produced genome-edited MSCs that secreted dual chemokine granulocyte chemotactic protein-2 (GCP-2) and stromal-derived factor-1α (SDF-1α) and determined their therapeutic potential in the context of experimental ischemia. METHODS: GCP-2 and SDF-1α genes were integrated into safe harbor site at the safe harbor genomic locus of amniotic mesenchymal stem cells (AMM) via transcription activator-like effector nucleases (TALEN)...
June 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29494891/maternal-immune-activation-induced-by-lipopolysaccharide-triggers-immune-response-in-pregnant-mother-and-fetus-and-induces-behavioral-impairment-in-adult-rats
#14
Lutiana Roque Simões, Gustavo Sangiogo, Michael Hikaru Tashiro, Jaqueline S Generoso, Cristiano Julio Faller, Diogo Dominguini, Gustavo Antunes Mastella, Giselli Scaini, Vijayasree Vayalanellore Giridharan, Monique Michels, Drielly Florentino, Fabricia Petronilho, Gislaine Zilli Réus, Felipe Dal-Pizzol, Alexandra I Zugno, Tatiana Barichello
Evidence suggest that prenatal immune system disturbance contributes largely to the pathophysiology of neuropsychiatric disorders. We investigated if maternal immune activation (MIA) could induce inflammatory alterations in fetal brain and pregnant rats. Adult rats subjected to MIA also were investigated to evaluate if ketamine potentiates the effects of infection. On gestational day 15, Wistar pregnant rats received lipopolysaccharide (LPS) to induce MIA. After 6, 12 and 24 h, fetus brain, placenta, and amniotic fluid were collected to evaluate early effects of LPS...
May 2018: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29486853/oxidative-stress-induces-senescence-and-sterile-inflammation-in-murine-amniotic-cavity
#15
Jossimara Polettini, Lauren S Richardson, Ramkumar Menon
OBJECTIVE: A physiologic increase of reactive oxygen species (ROS) is observed through pregnancy. ROS-induced damage to major cellular elements, specifically protein peroxidation, can lead to fetal and placental tissue senescence and inflammation often associated with normal parturition. The purpose of this study was to examine the effects of oxidative stress (OS) in inducing changes in proteins, senescence, and sterile inflammation in pregnant mice. METHODS: CD-1 mice (n = 5/group) on day 14 of gestation were subjected to minilaparotomy and the uterine horn between gestational sacs was injected with the following: saline (control), cigarette smoke extract (CSE) CSE diluted in saline and CSE + SB 203580 (SB) (a p38 mitogen-activated protein kinase (MAPK) inhibitor)...
March 2018: Placenta
https://www.readbyqxmd.com/read/29483819/risks-of-using-sterilization-by-gamma-radiation-the-other-side-of-the-coin
#16
REVIEW
C Randall Harrell, Valentin Djonov, Crissy Fellabaum, Vladislav Volarevic
The standard sterilization method for most medical devices over the past 40 years involves gamma irradiation. During sterilization, gamma rays efficiently eliminate microorganisms from the medical devices and tissue allografts, but also significantly change molecular structure of irradiated products, particularly fragile biologics such as cytokines, chemokines and growth factors. Accordingly, gamma radiation significantly alters biomechanical properties of bone, tendon, tracheal, skin, amnion tissue grafts and micronized amniotic membrane injectable products...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29466434/nutritional-intra-amniotic-therapy-increases-survival-in-a-rabbit-model-of-fetal-growth-restriction
#17
Hatice Gulcin Gumus, Miriam Illa, Laura Pla, Monica Zamora, Fatima Crispi, Eduard Gratacos
OBJECTIVE: To evaluate the perinatal effects of a prenatal therapy based on intra-amniotic nutritional supplementation in a rabbit model of intrauterine growth restriction (IUGR). METHODS: IUGR was surgically induced in pregnant rabbits at gestational day 25 by ligating 40-50% of uteroplacental vessels of each gestational sac. At the same time, modified-parenteral nutrition solution (containing glucose, amino acids and electrolytes) was injected into the amniotic sac of nearly half of the IUGR fetuses (IUGR-T group n = 106), whereas sham injections were performed in the rest of fetuses (IUGR group n = 118)...
2018: PloS One
https://www.readbyqxmd.com/read/29408475/optimal-route-of-diphtheria-toxin-administration-to-eliminate-native-nephron-progenitor-cells-in-vivo-for-kidney-regeneration
#18
COMPARATIVE STUDY
Shohei Fukunaga, Shuichiro Yamanaka, Toshinari Fujimoto, Susumu Tajiri, Taketo Uchiyama, Kei Matsumoto, Takafumi Ito, Kazuaki Tanabe, Takashi Yokoo
To address the lack of organs for transplantation, we previously developed a method for organ regeneration in which nephron progenitor cell (NPC) replacement is performed via the diphtheria toxin receptor (DTR) system. In transgenic mice with NPC-specific expression of DTR, NPCs were eliminated by DT and replaced with NPCs lacking the DTR with the ability to differentiate into nephrons. However, this method has only been verified in vitro. For applications to natural models, such as animal fetuses, it is necessary to determine the optimal administration route and dose of DT...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29402914/neuroprotection-of-the-hypoxic-ischemic-mouse-brain-by-human-cd117-cd90-cd105-amniotic-fluid-stem-cells
#19
Michelangelo Corcelli, Kate Hawkins, Filipa Vlahova, Avina Hunjan, Kate Dowding, Paolo De Coppi, Anna L David, Donald Peebles, Pierre Gressens, Henrik Hagberg, Mariya Hristova, Pascale V Guillot
Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43-45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination...
February 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29393249/upregulation-of-vascular-endothelial-growth-factor-in-amniotic-fluid-stem-cells-enhances-their-potential-to-attenuate-lung-injury-in-a-preterm-rabbit-model-of-bronchopulmonary-dysplasia
#20
Julio Jiménez, Flore Lesage, Jute Richter, Taro Nagatomo, Thomas Salaets, Silvia Zia, Marina Gabriela Mori Da Cunha, Jeroen Vanoirbeek, Jan A Deprest, Jaan Toelen
BACKGROUND: Bronchopulmonary dysplasia (BPD) is a chronic lung disease that affects extremely preterm infants and remains - despite improvements in neonatal intensive care - a major cause of neonatal mortality and morbidity. Cell-therapeutic strategies employing mesenchymal stem cells (MSC) have been shown to modulate lung development in BPD models. OBJECTIVE: Herein, we evaluate the potential of human amniotic fluid (hAF)-SC and hAF-SC with upregulated expression of vascular endothelial growth factor (VEGF) as cell-therapeutic agents for BPD...
2018: Neonatology
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