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Amniotic injection

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https://www.readbyqxmd.com/read/29132800/fetal-bone-marrow-homing-of-donor-mesenchymal-stem-cells-after-transamniotic-stem-cell-therapy-trascet
#1
Hester F Shieh, Azra Ahmed, Sarah A Tracy, David Zurakowski, Dario O Fauza
PURPOSE: Donor cell engraftment patterns following transamniotic stem cell therapy (TRASCET) with amniotic fluid mesenchymal stem cells (afMSCs) are incompatible with solely direct amniotic seeding. We sought to determine whether fetal bone marrow is a component of such engraftment and to examine the chronology of afMSC placental trafficking. METHODS: Two groups of Sprague-Dawley rat fetuses received volume-matched intraamniotic injections on gestational day 17 (E17; term E22): either afMSCs labeled with a luciferase reporter gene or luciferase protein alone...
October 12, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29117499/preclinical-chorioamnionitis-dysregulates-cxcl1-cxcr2-signaling-throughout-the-placental-fetal-brain-axis
#2
REVIEW
Tracylyn R Yellowhair, Shahani Noor, Jessie R Maxwell, Christopher V Anstine, Akosua Y Oppong, Shenandoah Robinson, Erin D Milligan, Lauren L Jantzie
In the United States, perinatal brain injury (PBI) is a major cause of infant mortality and childhood disability. For a large proportion of infants with PBI, central nervous system (CNS) injury begins in utero with inflammation (chorioamnionitis/CHORIO) and/or hypoxia-ischemia. While studies show CHORIO contributes to preterm CNS injury and is also a common independent risk factor for brain injury in term infants, the molecular mechanisms mediating inflammation in the placental-fetal-brain axis that result in PBI remain a gap in knowledge...
November 5, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/29077951/in-ovo-feeding-of-l-arginine-alters-energy-metabolism-in-post-hatch-broilers
#3
L L Yu, T Gao, M M Zhao, P A Lv, L Zhang, J L Li, Y Jiang, F Gao, G H Zhou
This study aimed to investigate the effects of in ovo feeding (IOF) of L-arginine (Arg) on energy metabolism in post-hatch broilers. A total of 720 eggs was randomly assigned to 3 treatments: 1) non-injected control group, 2) 0.75% NaCl diluent-injected control group, and 3) 1.0% Arg solution-injected group. At 17.5 d of incubation, 0.6 mL of each solution was injected into the amniotic fluid of each egg of injected groups. After hatching, 80 male chicks were randomly assigned to each treatment group with 8 replicates per group...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29077950/effects-of-in-ovo-feeding-of-cationic-amino-acids-on-hatchability-hatch-weights-and-organ-developments-in-domestic-pigeon-squabs-columba-livia
#4
X Y Zhang, L L Li, L P Miao, N N Zhang, X T Zou
This study was conducted to evaluate the effect of in ovo feeding of cationic amino acids on hatchability, hatch weights, and organ developments in pigeon squabs. Two experiments were conducted in this study. Eggs in Exp. 1 were subjected to modification of in ovo feeding in pigeons. Optimal time was determined by checking amniotic fluid volume, and suitable length was confirmed through ink injection. Results showed that the optimum time of in ovo feeding was on d 13 of embryonic development, and the suitable injected length was 20 mm to reach the amniotic cavity of the embryo...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29049352/exposure-to-intrauterine-inflammation-alters-metabolomic-profiles-in-the-amniotic-fluid-fetal-and-neonatal-brain-in-the-mouse
#5
Amy G Brown, Natalia M Tulina, Guillermo O Barila, Michael S Hester, Michal A Elovitz
INTRODUCTION: Exposure to prenatal inflammation is associated with diverse adverse neurobehavioral outcomes in exposed offspring. The mechanism by which inflammation negatively impacts the developing brain is poorly understood. Metabolomic profiling provides an opportunity to identify specific metabolites, and novel pathways, which may reveal mechanisms by which exposure to intrauterine inflammation promotes fetal and neonatal brain injury. Therefore, we investigated whether exposure to intrauterine inflammation altered the metabolome of the amniotic fluid, fetal and neonatal brain...
2017: PloS One
https://www.readbyqxmd.com/read/29048644/human-amniotic-epithelial-cells-inhibit-growth-of-epithelial-ovarian-cancer-cells-via-tgf%C3%A2-%C3%AE-1-mediated-cell-cycle-arrest
#6
Shixia Bu, Qiuwan Zhang, Qian Wang, Dongmei Lai
It is reported that human amniotic epithelial cells (hAECs) endow intrinsic antitumor effects on certain kinds of cancer. This research was designed to evaluate whether hAECs endowed potential anticancer properties on epithelial ovarian cancer (EOC) cells in vivo and in vitro, which has not been reported before. In this study, we established a xenografted BALB/c nude mouse model by subcutaneously co-injecting ovarian cancer cell line, SK-OV-3, and hAECs for 28 days. In ex vivo experiments, CCK‑8 cell viability assay, real-time PCR, cell counting assay, cell cycle analysis and immunohistochemistry (IHC) assay were used to detect the effects of hAEC‑secreted factors on the proliferation and cell cycle progression of EOC cells...
September 14, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28967074/palladium-103-plaque-radiation-therapy-for-ciliary-body-melanoma-through-a-functioning-glaucoma-filtering-bleb
#7
Arif H K Pathan, Alexander Barash, Lawrence B Tena, Paul T Finger
PURPOSE: To provide a clinical description of the long-term outcome of a 103Pd plaque-irradiated ciliary body melanoma with extrascleral extension while attempting to preserve a subadjacent glaucoma filtering bleb. METHODS: A 75-year-old woman with pseudoexfoliative glaucoma for 17 years, 16 years status post argon laser trabeculoplasty, and 15 years status post trabeculectomy in the left eye, was diagnosed with an ipsilateral ciliary body melanoma with visible extrascleral extension...
September 23, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28882138/a-dual-therapy-of-off-pump-temporary-left-ventricular-extracorporeal-device-and-amniotic-stem-cell-for-cardiogenic-shock
#8
Toshinobu Kazui, Phat L Tran, Tia R Pilikian, Katie M Marsh, Raymond Runyan, John Konhilas, Richard Smith, Zain I Khalpey
BACKGROUND: Temporary mechanical circulatory support device without sternotomy has been highly advocated for severe cardiogenic shock patient but little is known when coupled with amniotic stem cell therapy. CASE PRESENTATION: This case reports the first dual therapy of temporary left ventricular extracorporeal device CentriMag with distal banding technique and human amniotic stem cell injection for treating a severe refractory cardiogenic shock of an 68-year-old female patient...
September 7, 2017: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/28873045/gadolinium-chelate-safety-in-pregnancy-barely-detectable-gadolinium-levels-in-the-juvenile-nonhuman-primate-after-in-utero-exposure
#9
Joao Prola-Netto, Mark Woods, Victoria H J Roberts, Elinor L Sullivan, Christina Ann Miller, Antonio E Frias, Karen Y Oh
Purpose To determine whether gadolinium remains in juvenile nonhuman primate tissue after maternal exposure to intravenous gadoteridol during pregnancy. Materials and Methods Gravid rhesus macaques and their offspring (n = 10) were maintained, as approved by the institutional animal care and utilization committee. They were prospectively studied as part of a pre-existing ongoing research protocol to evaluate the effects of maternal malnutrition on placental and fetal development. On gestational days 85 and 135, they underwent placental magnetic resonance imaging after intravenous gadoteridol administration...
September 4, 2017: Radiology
https://www.readbyqxmd.com/read/28856020/can-ultrasound-be-helpful-in-selecting-optimal-management-methods-for-pregnancies-complicated-by-placental-non-trophpblastic-tumors
#10
REVIEW
Nabil Abdalla, Robert Piórkowski, Paweł Stanirowski, Monika Pazura, Krzysztof Cendrowski, Włodzimierz Sawicki
Placental chorioangioma is the most common subtype of non-trophoblastic placental tumors. Other subtypes are very rare and usually associated with an uneventful course of pregnancy. Most chorioangiomas are small and of no clinical significance. Giant chorioangiomas may be associated with serious fetal and maternal complications. So far, no established ultrasound guidelines are available for the management of placental non-trophoblastic tumors. This may be attributed to the rarity of the disease entity and its different clinical features and complications...
June 2017: Journal of Ultrasonography
https://www.readbyqxmd.com/read/28842991/the-amniotic-fluid-as-a-source-of-mesenchymal-stem-cells-with-lung-specific-characteristics
#11
Flore Lesage, Silvia Zia, Julio Jiménez, Jan Deprest, Jaan Toelen
The amniotic fluid is a clinically accessible source of mesenchymal stem cells (AF-MSC) during gestation, which enables autologous cellular therapy for perinatal disorders. The origin of AF-MSC remains elusive: renal and neuronal progenitors have been isolated from the AF-MSC pool, yet no cells with pulmonary characteristics. We analyzed gene expression of pulmonary and renal markers of 212 clonal lines of AF-MSC isolated from amniocentesis samples. AF-MSC were cultured on dishes coated with extracellular matrix (ECM) proteins from decellularized fetal rabbit lungs...
August 26, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28828329/next-generation-sequencing-and-karyotype-analysis-for-the-diagnosis-of-robertsonian-translocation-type-trisomy-13-a-case-report
#12
Jing Sha, Fumin Liu, Bei Zhang, Yang Huang, Qinglin Zhang, Gao Juan, Jingfang Zhai
Trisomy 13 (Patau syndrome) is the third most common autosomal trisomy with a prevalence between 1 in 10,000 - 20,000 live births. Robertsonian translocations represent the largest number of chromosomal aberrations in human population with an incidence of 1.23 in 1000 live birth and translocation 13;14 is one of the most frequent Robertsonian translocations (approximately 75%). We sampled umbilical vein blood from a 27-yr-old woman whose ultrasonography findings revealed congenital heart defects, single ventricle, polycystic kidney, median cleft lip and palate and holoprosencephaly at gestational age of 23+6 weeks for karyotype and sequencing during intra-amniotic cavity injection of acrinol for labor induction...
June 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28754012/intra-amniotic-administration-gallus-gallus-of-cicer-arietinum-and-lens-culinaris-prebiotics-extracts-and-duck-egg-white-peptides-affects-calcium-status-and-intestinal-functionality
#13
Tao Hou, Nikolai Kolba, Raymond P Glahn, Elad Tako
Calcium (Ca) is one of the most abundant inorganic elements in the human body and has many important physiological roles. Prebiotics and bioactive peptides are two important substances used to promote calcium uptake. However, the difference in mechanisms of the calcium uptake from these two supplements is not clear. By using the Gallus gallus model and the intra-amniotic administration procedure, the aim of this study was to investigate whether Ca status, intestinal functionality, and health-promoting bacterial populations were affected by prebiotics extracted from chickpea and lentil, and duck egg white peptides (DPs)...
July 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28744964/specification-of-larval-axes-of-partial-embryos-in-the-temnopleurid-temnopleurus-toreumaticus-and-the-strongylocentroid-hemicentrotus-pulcherrimus
#14
Chisato Kitazawa, Michihiro Nakano, Tadashi Yamaguchi, Chisato Miyahara, Akira Yamanaka
Many sea urchins, including the strongylocentroid Hemicentrotus pulcherrimus, produce an amniotic cavity on the left for adult rudiment formation at the late larval stage. In contrast, temnopleurids form a cell mass at the early larval stage instead of an amniotic cavity. Although the mechanisms establishing left-right polarity of the amniotic cavity involve cell-cell interactions and signaling pathways, corresponding pathways for the cell mass are unknown. We analyzed the effects of blastomere isolation on the specification of larval axes in the temnopleurid Temnopleurus toreumaticus and compared them to those in H...
September 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28727112/effect-of-in-ovo-feeding-of-l-arginine-on-the-hatchability-growth-performance-gastrointestinal-hormones-and-jejunal-digestive-and-absorptive-capacity-of-posthatch-broilers
#15
T Gao, M Zhao, L Zhang, J Li, L Yu, P Lv, F Gao, G Zhou
This study was conducted to investigate the effects of in ovo feeding (IOF) of Arg solution on the hatchability, growth performance, gastrointestinal hormones, serum AA, activities of digestive enzymes, and mRNA expressions of sensing receptors and nutrient transporters in the jejunum of posthatch broilers. One thousand two hundred embryonated eggs with similar weight were randomly allocated to 5 groups consisting of 8 replicates of 40 eggs each. The 5 treatments were arranged as a noninjected control, a diluent-injected (0...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28719908/ultrasound-guided-intrauterine-labeling-of-rat-fetuses
#16
Hatice Gulcin Gumus, Miriam Illa, Laura Pla, Anna Gonzalez, Elena Demicheva, David Ley, Fatima Crispi, Eduard Gratacos
AIM: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. METHODS: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared...
July 18, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28673594/management-of-temporomandibular-joint-degenerative-disorders-with-human-amniotic-membrane-hypothesis-of-action
#17
Luca Guarda-Nardini, Diletta Trojan, Adolfo Paolin, Daniele Manfredini
Approaches providing the positioning of human amniotic membrane (HAM) within the intra-articular space of arthritic TMJs have never been investigated. This contrasts with the increasing amount of evidence suggesting the potential positive effects of HAM on a number of surgical conditions, even included the interpositional arthroplasty for TMJ ankylosis. Thus, the possible usefulness of HAM to restore joint functions in severely damaged TMJs could be hypothesized. Based on these premises, the clinical research question "Is human amniotic membrane positioning effective to reduce symptoms and restore jaw function in patients with severe inflammatory-degenerative disorders of the temporomandibular joint?" has been addressed by performing a systematic review of the literature...
July 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28641395/effect-of-in-ovo-injection-of-corticotropin-releasing-hormone-on-the-timing-of-hatching-in-broiler-chickens
#18
Yugo Watanabe, Sylvia V H Grommen, Bert De Groef
In chicken embryos, intravenous injection of corticotropin-releasing hormone (CRH) causes the release of both corticosteroids and thyroid hormones. These hormones initiate and enhance the hatching process, raising the possibility that CRH treatment of the late chicken embryo could accelerate hatching and/or decrease the spread of hatching. We performed a series of exploratory tests to investigate whether in ovo delivery methods of CRH other than intravenous injection that are more practical in a commercial setting, affect hatching time in broilers...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28624056/evaluating-the-efficacy-of-different-types-of-stem-cells-in-preserving-gut-barrier-function-in-necrotizing-enterocolitis
#19
COMPARATIVE STUDY
Christopher J McCulloh, Jacob K Olson, Yijie Wang, Jennifer Vu, Sarah Gartner, Gail E Besner
BACKGROUND: Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality in premature infants. Increased intestinal permeability is central to NEC development. We have shown that stem cells (SCs) can reduce the incidence and severity of NEC. Our current goal was to investigate the efficacy of four different types of SC in preservation of gut barrier function during NEC. MATERIALS AND METHODS: We compared (1) amniotic fluid-derived mesenchymal SC, (2) bone marrow-derived mesenchymal SC, (3) amniotic fluid-derived neural SC, and (4) enteric neural SC...
June 15, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28602048/injected-corticosteroids-for-treating-plantar-heel-pain-in-adults
#20
REVIEW
Judy A David, Venkatesan Sankarapandian, Prince Rh Christopher, Ahana Chatterjee, Ashish S Macaden
BACKGROUND: Plantar heel pain, commonly resulting from plantar fasciitis, often results in significant morbidity. Treatment options include nonsteroidal anti-inflammatory drugs (NSAIDs), orthoses, physical therapy, physical agents (e.g. extracorporeal shock wave therapy (ESWT), laser) and invasive procedures including steroid injections. OBJECTIVES: To assess the effects (benefits and harms) of injected corticosteroids for treating plantar heel pain in adults. SEARCH METHODS: We searched the Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, the Cochrane Central Register of Controlled Trials (the Cochrane Library), MEDLINE, Embase, CINAHL, clinical trials registries and conference proceedings...
June 11, 2017: Cochrane Database of Systematic Reviews
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