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https://www.readbyqxmd.com/read/28239739/occurrence-of-veterinary-antibiotics-in-swine-manure-from-large-scale-feedlots-in-zhejiang-province-china
#1
Hua Wang, Yixuan Chu, Chengran Fang
The occurrence and distribution of five sulfonamides and three tetracyclines in swine manure sampled from large-scale feedlots in different areas of Zhejiang Province, China were detected using solid-phase extraction and high-performance liquid chromatography. All eight test antibiotics were detected in most of the manure samples. The dominant antibiotics in swine manure were sulfadiazine, sulfamerazine, sulfadimidine, tetracycline, and chlortetracycline. The maximum concentration of residual antibiotic reached up to 57...
February 27, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28238773/inhibition-of-the-tetk-efflux-pump-by-the-essential-oil-of-chenopodium-ambrosioides-l-and-%C3%AE-terpinene-against-staphylococcus-aureus-is-58
#2
Paulo W Limaverde, Fábia F Campina, Francisco A B da Cunha, Francidalva D Crispim, Fernando G Figueredo, Luciene F Lima, Cícera Datiane de M Oliveira-Tintino, Yedda M L S de Matos, Maria Flaviana B Morais-Braga, Irwin R A Menezes, Valdir Q Balbino, Henrique D M Coutinho, José P Siqueira-Júnior, Jackson R G S Almeida, Saulo R Tintino
The use of natural products is crucial to suppress the development of these micro-organisms and to reduce the concentration necessary to inhibit these microrganisms, reducing the toxicity risks also. In this study, the essential oil from Chenopodium ambrosioides Leaves and its main constituent α-Terpinene were used in the antibacterial and potentiating activity of antibiotics and ethidium bromide assays, against the bacterial strains Staphylococcus aureus IS-58, carriers of efflux pumps. The Minimum Inhibitory Concentration (MIC) was determined using a microdilution method...
February 23, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28238286/a-13-year-old-girl-with-pancytopenia-at-the-presentation-of-a-borrelia-hispanica-infection-a-case-report-and-review-of-the-literature
#3
Irmin Leen, Peggy Bruynseels, Benoît Kabamba Mukadi, Mark van Oort, Machiel van den Akker
BACKGROUND: It is not uncommon that a child with a febrile illness of unknown etiology is admitted to the hospital. When the complete blood count reveals a pancytopenia, the diagnostic process can be a real challenge. CASE PRESENTATION: A 13-year girl of Arab-Berber descent presented with abdominal pain and fever after a holiday in northwestern Morocco. A complete blood count revealed a pancytopenia and blood smear test results revealed spirochetes. Borrelia hispanica was identified by sequencing the 16S ribosomal ribonucleic acid gene...
February 27, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28237994/genetic-diversity-and-antibiotic-resistance-of-enterococcus-faecalis-isolates-from-traditional-korean-fermented-soybean-foods
#4
Jong-Hoon Lee, Donghun Shin, Bitnara Lee, Inhyung Lee, Do-Won Jeong
Eighty-five Enterococcus faecalis isolates collected from animals (40 isolates), Meju (Korean fermented soybean product; 27 isolates), humans (10 isolates), and various environmental samples (eight isolates) were subjected to multilocus sequence typing (MLST) to identify genetic differences between samples of different origins. MLST analysis resulted in 44 sequence types (STs), and the eBURST algorithm clustered the STs into 21 clonal complexes (CCs) and 17 singletons. The predominant STs, ST695 (21.1%, 18/85) and ST694 (9...
February 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28237599/prevalence-antimicrobial-resistance-and-molecular-characterization-of-campylobacter-spp-in-bulk-tank-milk-and-milk-filters-from-us-dairies
#5
Laura P Del Collo, Jeffrey S Karns, Debabrata Biswas, Jason E Lombard, Bradd J Haley, R Camilla Kristensen, Christine A Kopral, Charles P Fossler, Jo Ann S Van Kessel
Campylobacter spp. are frequently isolated from dairy cows as commensal organisms. Sporadic Campylobacter infections in humans in the United States are generally attributed to poultry, but outbreaks are also commonly associated with dairy products, particularly unpasteurized or raw milk. Bulk tank milk samples and milk filters from US dairy operations were collected during the National Animal Health Monitoring System Dairy 2014 study and analyzed using real-time PCR and traditional culture techniques for the presence of thermophilic Campylobacter species...
February 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28237036/mycobacterium-tuberculosis-rv1474c-is-a-tetr-like-transcriptional-repressor-that-regulates-aconitase-an-essential-enzyme-and-rna-binding-protein-in-an-iron-responsive-manner
#6
Kannan Balakrishnan, Krishnaveni Mohareer, Sharmistha Banerjee
Mycobacterium tuberculosis (M.tb), tuberculosis (TB) causing bacteria, employs several mechanisms to maintain iron homeostasis which is critical for its survival and pathogenesis. M.tb aconitase (Acn), a [4Fe-4S] cluster-containing essential protein, apart from participating in energy cycle, also binds to predicted iron-responsive RNA elements. In this study, we identified Rv1474c as a regulator of its operonic partner acn and carried out its biochemical and functional characterization. The binding motif for Rv1474c in the upstream region of acn (Rv1475c)-Rv1474c operon was verified by gel-shift assays...
March 2017: Tuberculosis
https://www.readbyqxmd.com/read/28230987/urinary-antibiotics-of-pregnant-women-in-eastern-china-and-cumulative-health-risk-assessment
#7
Hexing Wang, Na Wang, Junhua Qian, Lingyun Hu, Peixin Huang, Meifang Su, Xin Yu, Chaowei Fu, Feng Jiang, Qi Zhao, Ying Zhou, Haijiang Lin, Gengsheng He, Yue Chen, Qingwu Jiang
Exposure to antibiotics during pregnancy can pose a systematic effect on human health. A few bio-monitoring studies have demonstrated an extensive exposure of children to antibiotics, but there is still lack of data for pregnant women. To assess the exposure of pregnant women to antibiotics and potential health risk, we investigated 536 pregnant women aged 16-42 years from two geographically different study sites in Eastern China in 2015. We measured 21 antibiotics of five categories (seven fluoroquinolones, three phenicols, four tetracyclines, three macrolides, and four sulfonamides) in urines by using the isotope dilution ultra-performance liquid chromatography coupled to quadrupole time of-flight mass spectrometry...
February 23, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28230795/toxin-release-of-cyanobacterium-microcystis-aeruginosa-after-exposure-to-typical-tetracycline-antibiotic-contaminants
#8
Jing Ye, Yuping Du, Lumei Wang, Jingru Qian, Jiejing Chen, Qingwen Wu, Xiaojun Hu
The global usage of veterinary antibiotics is significant. Antibiotics can be released into aquatic environments and elicit toxic effects on non-target organisms. In this study, the growth characteristics and toxin release of the cyanobacterium Microcystis aeruginosa (M. aeruginosa) were examined to investigate the physiological effects of tetracycline antibiotics on aquatic life. Results showed that the degree of toxicities of the following target antibiotics was TC (tetracycline hydrochloride) > CTC (chlortetracycline hydrochloride) > OTC (oxytetracycline hydrochloride) in terms of growth parameters, EC10 (0...
February 21, 2017: Toxins
https://www.readbyqxmd.com/read/28229558/microbial-diversity-of-a-full-scale-uasb-reactor-applied-to-poultry-slaughterhouse-wastewater-treatment-integration-of-16s-rrna-gene-amplicon-and-shotgun-metagenomic-sequencing
#9
Tiago Palladino Delforno, Gileno Vieira Lacerda Júnior, Melline F Noronha, Isabel K Sakamoto, Maria Bernadete A Varesche, Valéria M Oliveira
The 16S rRNA gene amplicon and whole-genome shotgun metagenomic (WGSM) sequencing approaches were used to investigate wide-spectrum profiles of microbial composition and metabolic diversity from a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment. The data were generated by using MiSeq 2 × 250 bp and HiSeq 2 × 150 bp Illumina sequencing platforms for 16S amplicon and WGSM sequencing, respectively. Each approach revealed a distinct microbial community profile, with Pseudomonas and Psychrobacter as predominant genus for the WGSM dataset and Clostridium and Methanosaeta for the 16S rRNA gene amplicon dataset...
February 23, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28229236/asymmetric-bone-remodeling-in-mandibular-and-maxillary-tori
#10
Jean-Daniel Kün-Darbois, Bernard Guillaume, Daniel Chappard
OBJECTIVES: Tori are frequent paucisymptomatic bony outgrowths of the oral cavity in three locations: torus palatinus (TP), mandibularis (TM), and maxillaris (TMax). Their usually described histological characteristics are unspecific: normal cortical bone with more or less trabecular bone. The aim of this study was to describe tori's specific morphological and histomorphometric characteristics. MATERIALS AND METHODS: Histological characteristics in a series of 18 tori collected after surgical removal were analyzed...
February 23, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28224028/evaluation-of-antimicrobial-susceptibility-and-integron-carriage-in-helicobacter-pylori-isolates-from-patients
#11
Mehdi Goudarzi, Mohsen Heidary, Mehdi Azad, Maryam Fazeli, Hossein Goudarzi
AIM: The purpose of this study was to determine the antibiotic susceptibility pattern and distribution of integron in H. pylori isolates collected from patients referred to private health care centers in Tehran, Iran. BACKGROUND: Antibiotic resistance is the main reason for failure of Helicobacter pylori therapy. Integrons as genetic reservoirs play main roles in the dissemination of antimicrobial resistance gene. METHODS: During a 12-month cross-sectional study period, 65 H...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28223970/tet-c-gene-transfer-between-chlamydia-suis-strains-occurs-by-homologous-recombination-after-co-infection-implications-for-spread-of-tetracycline-resistance-among-chlamydiaceae
#12
Hanna Marti, Hoyon Kim, Sandeep J Joseph, Stacey Dojiri, Timothy D Read, Deborah Dean
Chlamydia suis is a swine pathogen that has also recently been found to cause zoonotic infections of the human eye, pharynx, and gastrointestinal tract. Many strains contain a tetracycline class C gene [tet(C)] cassette that confers tetracycline resistance. The cassette was likely originally acquired by horizontal gene transfer from a Gram-negative donor after the introduction of tetracycline into animal feed in the 1950s. Various research groups have described the capacity for different Chlamydia species to exchange DNA by homologous recombination...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28223459/evolution-and-epidemiology-of-multidrug-resistant-klebsiella-pneumoniae-in-the-united-kingdom-and-ireland
#13
Danesh Moradigaravand, Veronique Martin, Sharon J Peacock, Julian Parkhill
Klebsiella pneumoniae is a human commensal and opportunistic pathogen that has become a leading causative agent of hospital-based infections over the past few decades. The emergence and global expansion of hypervirulent and multidrug-resistant (MDR) clones of K. pneumoniae have been increasingly reported in community-acquired and nosocomial infections. Despite this, the population genomics and epidemiology of MDR K. pneumoniae at the national level are still poorly understood. To obtain insights into these, we analyzed a systematic large-scale collection of invasive MDR K...
February 21, 2017: MBio
https://www.readbyqxmd.com/read/28223380/vitamin-k3-induces-the-expression-of-the-stenotrophomonas-maltophilia-smevwx-multidrug-efflux-pump
#14
P Blanco, F Corona, M B Sánchez, J L Martínez
Stenotrophomonas maltophilia is an opportunistic pathogen, with increasing prevalence, able to cause infections in immunocompromised patients or in those with a previous pathology. The treatment of the infections caused by this bacterium is often complicated due to the several intrinsic antibiotic resistance mechanisms that it presents. Multidrug efflux pumps are among the best studied mechanisms of S. maltophilia antibiotic resistance. Some of these efflux pumps have a basal expression level but, in general, their expression is often low and only reaches high levels when the local regulator is mutated or bacteria are in the presence of an effector...
February 21, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28222842/antimicrobial-use-for-and-resistance-of-zoonotic-bacteria-recovered-from-nonhuman-primates
#15
Jeffrey Kim, Dondrae J Coble, Gregory W Salyards, Julie K Bower, William J Rinaldi, Gail B Plauche, Gregory G Habing
As a growing threat to human and animal health, antimicrobial resistance (AMR) has become a central public-health topic. Largescale surveillance systems, such as the National Antimicrobial Resistance Monitoring System (NARMS), are now established to monitor and provide guidance regarding AMR, but comprehensive literature on AMR among NHP is sparse. This study provides data regarding current antimicrobial use strategies and the prevalence of AMR in zoonotic bacteria recovered from NHP within biomedical research institutions...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28222270/the-effects-of-pig-manure-application-on-the-spread-of-tetracycline-resistance-in-bulk-and-cucumber-rhizosphere-soils-a-greenhouse-experiment
#16
Yijun Kang, Yangyang Hao, Dan Xia, Min Shen, Qing Li, Jian Hu
Understanding the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils is important to evaluate the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, tetracycline-resistant E. coli (TRE), tetracycline-resistant Pseudomonas spp. (TRP), as well as TRGs between bulk and cucumber rhizosphere soils. The application of pig manure resulted in the long-term persistence of TRB, TRE, TRP and TRGs in bulk soil and rhizosphere of cucumber for at least 65 days...
February 21, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28221963/prevalence-and-antimicrobial-resistance-of-enterobacteriaceae-in-shell-eggs-from-small-scale-poultry-farms-and-farmers-markets
#17
A Kilonzo-Nthenge, S N Nahashon, S Godwin, S Liu, D Long
Public health concerns over the emergence of antimicrobial resistant bacteria have increased recently. The purpose of this study was to investigate the prevalence of antimicrobial resistant Enterobacteriaceae in shell eggs purchased from small poultry farms and farmers' markets. A total of 504 eggs were pooled to make 252 composite samples, consisting of 2 eggs per composite. The microbial quality of shell eggs was determined by standard quantitative, biochemical, and PCR techniques. Susceptibility to 13 antimicrobial agents was determined by the Kirby-Bauer disk diffusion technique, and results were interpreted based on Clinical and Laboratory Standards Institute values...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221939/load-and-prevalence-of-antimicrobial-resistant-escherichia-coli-from-fresh-goat-meat-in-arusha-tanzania
#18
Gaspary Mwanyika, Douglas R Call, Benardether Rugumisa, Catherine Luanda, Rehema Murutu, Murugan Subbiah, Joram Buza
Given the potential public health risks associated with a burgeoning goat meat industry in Tanzania, we estimated the load of Escherichia coli and the prevalence of antibiotic-resistant strains for goat meat by using a cross-sectional study design (June to July 2015). Five large (n = 60 samples) and five small (n = 64 samples) slaughterhouses were sampled over a period of four to six visits each. Meat rinsate was prepared and plated onto MacConkey agar, and presumptive E. coli colonies were enumerated and reported as CFU per milliliter of rinsate...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221925/genetic-diversity-and-antibiotic-resistance-of-escherichia-coli-isolates-from-different-leafy-green-production-systems
#19
Mosimanegape Jongman, Lise Korsten
Foodborne disease outbreaks linked to contaminated irrigation water and fresh produce are a public health concern. The presence of Escherichia coli isolates from irrigation water and leafy green vegetables in different food production systems (large commercial farms, small-scale farms, and homestead gardens) was investigated. The prevalence of antibiotic resistance and virulence in these isolates was further assessed, and links between water source and irrigated crops were identified using antimicrobial and genotypic analyses...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221916/characterization-and-antimicrobial-resistance-of-salmonella-typhimurium-isolates-from-clinically-diseased-pigs-in-korea
#20
Sang-Ik Oh, Jong Wan Kim, Myeongju Chae, Ji-A Jung, Byungjae So, Bumseok Kim, Ha-Young Kim
This study investigated the prevalence of Salmonella enterica serovar and antimicrobial resistance in Salmonella Typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in Korea. Isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. Among 94 Salmonella isolates, 81 (86.2%) were identified as being of the Salmonella Typhimurium serotype, followed by Salmonella Derby (6 of 94, 6.4%), Salmonella 4,[5],12:i:- (4 of 94, 4...
November 2016: Journal of Food Protection
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