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https://www.readbyqxmd.com/read/28221974/evaluation-of-a-surface-sampling-method-for-recovery-of-human-noroviruses-prior-to-detection-using-reverse-transcription-quantitative-pcr
#1
Grace Tung-Thompson, Blanca I Escudero-Abarca, Janie Outlaw, Arnaud Ganee, Sylvanie Cassard, Claude Mabilat, Lee-Ann Jaykus
Human noroviruses are the most common cause of acute viral gastroenteritis, and the environmental persistence of these viruses contributes to their transmissibility. Environmental sampling is thus an important tool for investigating norovirus outbreaks and for assessing the effectiveness of cleaning and decontamination regimens. The purpose of this study was to evaluate a sampling material (wipes) for their efficacy at recovering human norovirus from hard surfaces and foods. Dilutions of a human norovirus GII...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28196336/regression-of-atherosclerosis-with-apple-procyanidins-by-activating-the-atp-binding-cassette-subfamily-a-member-1-in-a-rabbit-model
#2
Liang Wang, Toshio Fumoto, Saeko Masumoto, Toshihiko Shoji, Tomisato Miura, Masato Naraoka, Naoya Matsuda, Tadaatsu Imaizumi, Hiroki Ohkuma
BACKGROUND AND AIMS: Apple polyphenol contains abundant procyanidins, which have been associated with an anti-atherosclerosis and cholesterol-lowering effect. The aim of this study was to investigate whether apple procyanidins (APCs) feature therapeutic efficacy in terms of regressing atherosclerosis and whether this efficacy is due to mechanisms other than a cholesterol-lowering effect. METHODS: After eight weeks on an atherogenic diet, rabbits were given a normal diet for another eight weeks to normalize the increased serum lipids level...
January 27, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28194717/metal-chelate-immobilization-of-lipase-onto-polyethylenimine-coated-mcm-41-for-apple-flavor-synthesis
#3
Armin Sadighi, Seyed Farshad Motevalizadeh, Morteza Hosseini, Ali Ramazani, Lena Gorgannezhad, Hamid Nadri, Behnaz Deiham, Mohammad Reza Ganjali, Abbas Shafiee, Mohammad Ali Faramarzi, Mehdi Khoobi
An enzyme immobilized on a mesoporous silica nanoparticle can serve as a multiple catalyst for the synthesis of industrially useful chemicals. In this work, MCM-41 nanoparticles were coated with polyethylenimine (MCM-41@PEI) and further modified by chelation of divalent metal ions (M = Co(2+), Cu(2+), or Pd(2+)) to produce metal-chelated silica nanoparticles (MCM-41@PEI-M). Thermomyces lanuginosa lipase (TLL) was immobilized onto MCM-41, MCM-41@PEI, and MCM-41@PEI-M by physical adsorption. Maximum immobilization yield and efficiency of 75 ± 3...
February 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28140510/improving-risk-assessment-of-color-additives-in-medical-device-polymers
#4
Vaishnavi Chandrasekar, Dustin W Janes, Christopher Forrey, David M Saylor, Akhil Bajaj, Timothy V Duncan, Jiwen Zheng, Kausar B Riaz Ahmed, Brendan J Casey
Many polymeric medical device materials contain color additives which could lead to adverse health effects. The potential health risk of color additives may be assessed by comparing the amount of color additive released over time to levels deemed to be safe based on available toxicity data. We propose a conservative model for exposure that requires only the diffusion coefficient of the additive in the polymer matrix, D, to be specified. The model is applied here using a model polymer (poly(ether-block-amide), PEBAX 2533) and color additive (quinizarin blue) system...
January 31, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28130877/mimicking-physiological-flow-conditions-to-study-alterations-of-bioactive-glass-surfaces-in-vitro
#5
Miriam Höner, Frederik Böke, Michael Weber, Horst Fischer
Bioactive glasses form a strong bond with surrounding tissue and slowly degrade when implanted in vivo, stimulating the host bone to regenerate itself. We investigated the behaviour of microstructured bioactive glass surfaces (13-93) in an SBF reactor, which mimics physiological flow conditions. The structures were developed to potentially influence cell-biological long term processes such as osteogenic differentiation. It is therefore important that the structures withstand a certain time in SBF or body fluids...
January 27, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28130836/optimized-performance-of-the-integrated-hepatic-cell-loaded-cryogel-based-bioreactor-with-intermittent-perfusion-of-acute-liver-failure-plasma
#6
Apeksha Damania, Anupam Kumar, Shiv K Sarin, Ashok Kumar
Acute liver failure (ALF) plasma has cytotoxic effects on the cell-loaded bioreactor in bioartificial liver support systems due to the presence of innumerable hepatotoxic compounds that adversely affect the morphology and functionality of the cells. We have designed a hybrid bioreactor that integrates a hepatic cell-loaded cryogel disc and an activated carbon cloth in one compact unit, with potential application as a bioartificial liver support. In this article, we assess the performance of this integrated hybrid cryogel-based bioreactor in a perfusion-based culture system and analyze its functionality and longevity in the presence of intermittent exposure to ALF plasma...
January 27, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28103422/vitamin-supplementation
#7
EDITORIAL
P B Persson, A B Persson
No abstract text is available yet for this article.
March 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28101467/some-qualitative-and-rheological-properties-of-virgin-olive-oil-apple-vinegar-salad-dressing-stabilized-with-xanthan-gum
#8
Solmaz Abedinzadeh, Mohammadali Torbati, Sodeif Azadmard-Damirchi
Purpose: Lipid oxidation and rheological properties are the main qualitative parameters determined in food emulsions. Salad dressings are food emulsions important in our daily diet, but conventional salad dressings have high amounts of cholesterol and saturated fatty acids because of egg yolk in their formulations. There are many studies on the modification of salad dressing formulations to replace egg yolk and saturated fats. The present study describes new formulation of salad dressing with olive oil and apple vinegar to produce a functional food product...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28031473/relative-efficacy-of-two-clades-of-aphelinus-mali-hymenoptera-aphelinidae-for-control-of-woolly-apple-aphid-hemiptera-aphididae-in-china
#9
Min Su, Xiumei Tan, Qinmin Yang, Hongxu Zhou, Fanghao Wan
Aphelinus mali (Haldeman) is an effective natural enemy used in China to control woolly apple aphid. Previous studies have found that populations of A. mali in China fall into two distinct genetic clades. We found that one of these, the Shandong clade, is more effective as a biological control agent than the Liaoning clade. The control ability of the Shandong clade of A. mali (a/Th = 2.447) was found to be stronger than that of the Liaoning clade (a/Th = 1.278); the search parameter (Q) and the mutual interference parameter (m) of the Shandong clade (0...
December 28, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28008718/binary-solvent-based-ionic-liquid-assisted-surfactant-enhanced-emulsification-microextraction-for-the-determination-of-four-fungicides-in-apple-juice-and-apple-vinegar
#10
Xiaochu Chen, Xu Zhang, Fengmao Liu, Fan Hou
A binary-solvent-based ionic-liquid-assisted surfactant-enhanced emulsification microextraction method was developed for the separation/preconcentration and determination of four fungicides (pyrimethanil, fludioxonil, cyprodynil, pyraclostrobin) in apple juice and apple vinegar. A nonchlorinated solvent amyl acetate, which has a lower density than water, was used as the extraction solvent, and an ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate, which has a high density and low toxicity, was used as a secondary solvent mixed with the extraction solvent...
December 23, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27925384/co-micellized-pluronic-mixture-with-thermo-sensitivity-and-residence-stability-as-an-injectable-tissue-adhesion-barrier-hydrogel
#11
Se Heang Oh, Jun Goo Kang, Jin Ho Lee
Although the tissue adhesion which leads to various complications frequently occurs after surgery, the development of an ideal tissue adhesion barrier is still a challenge. In this study, a thermo-sensitive hydrogel, which can fulfill the essential requirements of tissue adhesion barrier (that is, ease of handling for surgeon, flowing down prevention after application, stable residence on the injury during wound healing, and no use of toxic additives), was developed using biocompatible polyethylene glycol-polypropylene glycol copolymers (Pluronic F127/F68/P123 mixture)...
December 7, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27890695/nitric-oxide-polyamines-cross-talk-during-dormancy-release-and-germination-of-apple-embryos
#12
Urszula Krasuska, Katarzyna Ciacka, Agnieszka Gniazdowska
Nitric oxide (NO) and polyamines (PAs) belong to plant growth and development regulators. These compounds play a key role in numerous physiological processes e.g. seed germination. Based on the suggestion of overlapping of NO and PAs biosynthetic pathways, we demonstrated a cross-talk of NO and PAs in regulation of embryonic dormancy release. The aim of the work was to investigate an impact of PAs (Put, Spd and Spm) or NO short-term fumigation on nitrite, urea, Arg and ornithine (Orn) content, NO synthase-like (NOS-like) and arginase activity in axes of apple (Malus domestica Borkh...
November 23, 2016: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27889932/interaction-of-hepes-buffer-with-glass-ceramic-scaffold-can-hepes-replace-tris-in-sbf
#13
Dana Rohanová, Diana Horkavcová, Laine Paidere, Aldo Roberto Boccaccini, Pavlína Bozděchová, Petr Bezdička
An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good's buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27862914/in-vivo-biocompatibility-vascularization-and-incorporation-of-integra-%C3%A2-dermal-regenerative-template-and-flowable-wound-matrix
#14
Thomas Später, Florian S Frueh, Wolfgang Metzger, Michael D Menger, Matthias W Laschke
Integra(®) matrix wound dressing (MWD) is used for the reconstruction of full-thickness skin defects. For the treatment of complex wounds, this dermal substitute is available as a flowable wound matrix (FWM) of identical composition. To clarify whether variations in sample preparation and consistency affect the biocompatibility and tissue incorporation, we herein compared MWD and FWM. The matrices were characterized using scanning electron microscopy and histology. Moreover, they were implanted in mouse dorsal skinfold chambers to analyze their in vivo performance over 2 weeks...
November 14, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27820667/dissipation-of-difenoconazole-in-apples-used-for-production-of-baby-food
#15
Ewa Szpyrka, Stanisław Walorczyk
Dissipation of fungicide difenoconazole (3-chloro-4-[(2RS,4RS;2RS,4SR)-4-methyl-2-(1H-1,2,4-triazol-1-ylmethyl)-1,3-dioxolan-2-yl]phenyl 4-chlorophenyl ether) was studied following its application on apples intended for production of baby food. The apples (varieties: Jonagold Decosta, Gala and Idared) were sprayed with the formulation to control pathogens causing fungal diseases: powdery mildew (Podosphaera leucotricha ELL et Ev./Salm.) and apple scab (Venturia inaequalis Cooke/Aderh.). A validated gas chromatography-based method with simultaneous electron capture and nitrogen phosphorus detection (GC-ECD/NPD) was used for the residue analysis...
February 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/27811203/rapid-rule-out-of-acute-myocardial-injury-using-a-single-high-sensitivity-cardiac-troponin-i-measurement
#16
Yader Sandoval, Stephen W Smith, Anoop S V Shah, Atul Anand, Andrew R Chapman, Sara A Love, Karen Schulz, Jing Cao, Nicholas L Mills, Fred S Apple
BACKGROUND: Rapid rule-out strategies using high-sensitivity cardiac troponin assays are largely supported by studies performed outside the US in selected cohorts of patients with chest pain that are atypical of US practice, and focused exclusively on ruling out acute myocardial infarction (AMI), rather than acute myocardial injury, which is more common and associated with a poor prognosis. METHODS: Prospective, observational study of consecutive patients presenting to emergency departments [derivation (n = 1647) and validation (n = 2198) cohorts], where high-sensitivity cardiac troponin I (hs-cTnI) was measured on clinical indication...
January 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/27796771/ii-insulin-processing-in-mitochondria
#17
María Del Carmen Camberos, Adriana A Pérez, Gisel A Passicot, Lucía C Martucci, María I Wanderley, Daniel P Udrisar, Juan C Cresto
Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat's diet of 25 g/rat of apple and 10 g/rat of hard-boiled eggs, 3 days a week. Mitochondrial insulin degradation was assayed with 5 % TCA, insulin antibody or Sephadex G50 chromatography...
October 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27795675/genomic-dissection-of-a-fuji-apple-cultivar-re-sequencing-snp-marker-development-definition-of-haplotypes-and-qtl-detection
#18
Miyuki Kunihisa, Shigeki Moriya, Kazuyuki Abe, Kazuma Okada, Takashi Haji, Takeshi Hayashi, Yoshihiro Kawahara, Ryutaro Itoh, Takeshi Itoh, Yuichi Katayose, Hiroyuki Kanamori, Toshimi Matsumoto, Satomi Mori, Harumi Sasaki, Takashi Matsumoto, Chikako Nishitani, Shingo Terakami, Toshiya Yamamoto
'Fuji' is one of the most popular and highly-produced apple cultivars worldwide, and has been frequently used in breeding programs. The development of genotypic markers for the preferable phenotypes of 'Fuji' is required. Here, we aimed to define the haplotypes of 'Fuji' and find associations between haplotypes and phenotypes of five traits (harvest day, fruit weight, acidity, degree of watercore, and flesh mealiness) by using 115 accessions related to 'Fuji'. Through the re-sequencing of 'Fuji' genome, total of 2,820,759 variants, including single nucleotide polymorphisms (SNPs) and insertions or deletions (indels) were detected between 'Fuji' and 'Golden Delicious' reference genome...
September 2016: Breeding Science
https://www.readbyqxmd.com/read/27785791/effect-of-probiotics-on-patulin-removal-from-synbiotic-apple-juice
#19
Alaleh Zoghi, Kianoush Khosravi-Darani, Sarah Sohrabvandi, Hossein Attar, Sayed Abolhasan Alavi
BACKGROUND: Studies have reported the occurrence of mycotoxin patulin in apple products. The aim of this study was to produce synbiotic apple juice and investigate the detoxification of patulin by Lactobacillus acidophilus and Lactobacillus plantarum as probiotic strains. Impact of seven process variables on efficiency of toxin removal was investigated using Plackett-Burman design and presence of the surface-layer proteins as binding site of probiotics to patulin was confirmed during 6 weeks cold storage...
October 27, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27755580/an-apple-fruit-fermentation-aff-treatment-improves-the-composition-of-the-rhizosphere-microbial-community-and-growth-of-strawberry-fragaria-%C3%A3-ananassa-duch-benihoppe-seedlings
#20
Jie Zhang, Hui Pang, Mengxia Ma, Yufen Bu, Wei Shao, Weijing Huang, Qianlong Ji, Yuncong Yao
Plant growth can be promoted by the application of apple fruit fermentation (AFF), despite unclear of the underlying mechanisms, the effects involved in AFF on rhizosphere microorganisms have been hypothesized. We investigated the consequences of applying AFF alone or in combination with Bacillus licheniformis to strawberry tissue culture seedlings in vitro, the analyses of Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rDNA were performed to determine AFF effects on rhizosphere. Moreover, the growth index and antioxidant enzyme activities were determined 30 days after treatments...
2016: PloS One
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