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https://www.readbyqxmd.com/read/27890695/nitric-oxide-polyamines-cross-talk-during-dormancy-release-and-germination-of-apple-embryos
#1
Urszula Krasuska, Katarzyna Ciacka, Agnieszka Gniazdowska
Nitric oxide (NO) and polyamines (PAs) belong to plant growth and development regulators. These compounds play a key role in numerous physiological processes e.g. seed germination. Based on the suggestion of overlapping of NO and PAs biosynthetic pathways, we demonstrated a cross-talk of NO and PAs in regulation of embryonic dormancy release. The aim of the work was to investigate an impact of PAs (Put, Spd and Spm) or NO short-term fumigation on nitrite, urea, Arg and ornithine (Orn) content, NO synthase-like (NOS-like) and arginase activity in axes of apple (Malus domestica Borkh...
November 23, 2016: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27889932/interaction-of-hepes-buffer-with-glass-ceramic-scaffold-can-hepes-replace-tris-in-sbf
#2
Dana Rohanová, Diana Horkavcová, Laine Paidere, Aldo Roberto Boccaccini, Pavlína Bozděchová, Petr Bezdička
An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good's buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27862914/in-vivo-biocompatibility-vascularization-and-incorporation-of-integra-%C3%A2-dermal-regenerative-template-and-flowable-wound-matrix
#3
Thomas Später, Florian S Frueh, Wolfgang Metzger, Michael D Menger, Matthias W Laschke
Integra(®) matrix wound dressing (MWD) is used for the reconstruction of full-thickness skin defects. For the treatment of complex wounds, this dermal substitute is available as a flowable wound matrix (FWM) of identical composition. To clarify whether variations in sample preparation and consistency affect the biocompatibility and tissue incorporation, we herein compared MWD and FWM. The matrices were characterized using scanning electron microscopy and histology. Moreover, they were implanted in mouse dorsal skinfold chambers to analyze their in vivo performance over 2 weeks...
November 14, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27820667/dissipation-of-difenoconazole-in-apples-used-for-production-of-baby-food
#4
Ewa Szpyrka, Stanisław Walorczyk
Dissipation of fungicide difenoconazole (3-chloro-4-[(2RS,4RS;2RS,4SR)-4-methyl-2-(1H-1,2,4-triazol-1-ylmethyl)-1,3-dioxolan-2-yl]phenyl 4-chlorophenyl ether) was studied following its application on apples intended for production of baby food. The apples (varieties: Jonagold Decosta, Gala and Idared) were sprayed with the formulation to control pathogens causing fungal diseases: powdery mildew (Podosphaera leucotricha ELL et Ev./Salm.) and apple scab (Venturia inaequalis Cooke/Aderh.). A validated gas chromatography-based method with simultaneous electron capture and nitrogen phosphorus detection (GC-ECD/NPD) was used for the residue analysis...
November 7, 2016: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/27811203/rapid-rule-out-of-acute-myocardial-injury-using-a-single-high-sensitivity-cardiac-troponin-i-measurement
#5
Yader Sandoval, Stephen W Smith, Anoop S V Shah, Atul Anand, Andrew R Chapman, Sara A Love, Karen Schulz, Jing Cao, Nicholas L Mills, Fred S Apple
BACKGROUND: Rapid rule-out strategies using high-sensitivity cardiac troponin assays are largely supported by studies performed outside the US in selected cohorts of patients with chest pain that are atypical of US practice, and focused exclusively on ruling out acute myocardial infarction (AMI), rather than acute myocardial injury, which is more common and associated with a poor prognosis. METHODS: Prospective, observational study of consecutive patients presenting to emergency departments [derivation (n = 1647) and validation (n = 2198) cohorts], where high-sensitivity cardiac troponin I (hs-cTnI) was measured on clinical indication...
November 3, 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27796771/ii-insulin-processing-in-mitochondria
#6
María Del Carmen Camberos, Adriana A Pérez, Gisel A Passicot, Lucía C Martucci, María I Wanderley, Daniel P Udrisar, Juan C Cresto
Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat's diet of 25 g/rat of apple and 10 g/rat of hard-boiled eggs, 3 days a week. Mitochondrial insulin degradation was assayed with 5 % TCA, insulin antibody or Sephadex G50 chromatography...
October 29, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27795675/genomic-dissection-of-a-fuji-apple-cultivar-re-sequencing-snp-marker-development-definition-of-haplotypes-and-qtl-detection
#7
Miyuki Kunihisa, Shigeki Moriya, Kazuyuki Abe, Kazuma Okada, Takashi Haji, Takeshi Hayashi, Yoshihiro Kawahara, Ryutaro Itoh, Takeshi Itoh, Yuichi Katayose, Hiroyuki Kanamori, Toshimi Matsumoto, Satomi Mori, Harumi Sasaki, Takashi Matsumoto, Chikako Nishitani, Shingo Terakami, Toshiya Yamamoto
'Fuji' is one of the most popular and highly-produced apple cultivars worldwide, and has been frequently used in breeding programs. The development of genotypic markers for the preferable phenotypes of 'Fuji' is required. Here, we aimed to define the haplotypes of 'Fuji' and find associations between haplotypes and phenotypes of five traits (harvest day, fruit weight, acidity, degree of watercore, and flesh mealiness) by using 115 accessions related to 'Fuji'. Through the re-sequencing of 'Fuji' genome, total of 2,820,759 variants, including single nucleotide polymorphisms (SNPs) and insertions or deletions (indels) were detected between 'Fuji' and 'Golden Delicious' reference genome...
September 2016: Breeding Science
https://www.readbyqxmd.com/read/27785791/effect-of-probiotics-on-patulin-removal-from-synbiotic-apple-juice
#8
Alaleh Zoghi, Kianoush Khosravi-Darani, Sarah Sohrabvandi, Hossein Attar, Sayed Abolhasan Alavi
BACKGROUND: Studies have reported the occurrence of mycotoxin patulin in apple products. The aim of this study was to produce synbiotic apple juice and investigate the detoxification of patulin by Lactobacillus acidophilus and Lactobacillus plantarum as probiotic strains. Impact of seven process variables on efficiency of toxin removal was investigated using Plackett-Burman design and presence of the surface-layer proteins as binding site of probiotics to patulin was confirmed during 6 weeks cold storage...
October 27, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27755580/an-apple-fruit-fermentation-aff-treatment-improves-the-composition-of-the-rhizosphere-microbial-community-and-growth-of-strawberry-fragaria-%C3%A3-ananassa-duch-benihoppe-seedlings
#9
Jie Zhang, Hui Pang, Mengxia Ma, Yufen Bu, Wei Shao, Weijing Huang, Qianlong Ji, Yuncong Yao
Plant growth can be promoted by the application of apple fruit fermentation (AFF), despite unclear of the underlying mechanisms, the effects involved in AFF on rhizosphere microorganisms have been hypothesized. We investigated the consequences of applying AFF alone or in combination with Bacillus licheniformis to strawberry tissue culture seedlings in vitro, the analyses of Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rDNA were performed to determine AFF effects on rhizosphere. Moreover, the growth index and antioxidant enzyme activities were determined 30 days after treatments...
2016: PloS One
https://www.readbyqxmd.com/read/27752249/walking-the-tightrope-counterproductive-work-behavior-as-compensation-for-citizenship-demands
#10
Andromachi Spanouli, Joeri Hofmans
Amidst a struggling economy, organizations are ruled by the survival of the fittest paradigm but it is the employees who tend to pay the price, with increased demands which, oftentimes, fall outside their job scope. In the present paper, we examined whether pressuring people to engage in such organizational citizenship behaviors (OCB) might in fact backfire and lead to increased Counterproductive Work Behavior (CWB) because of compensatory mechanisms. We propose a typology of OCB that distinguishes between discretionary and elicited OCB, hypothesizing that elicited but not discretionary OCB, positively relates to CWB...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/27731928/effects-of-a-cell-free-method-using-collagen-vitrigel-incorporating-tgf-%C3%AE-1-on-articular-cartilage-repair-in-a-rabbit-osteochondral-defect-model
#11
Hideyuki Maruki, Masato Sato, Toshiaki Takezawa, Yoshiki Tani, Munetaka Yokoyama, Takumi Takahashi, Eriko Toyoda, Eri Okada, Shigehisa Aoki, Joji Mochida, Yoshiharu Kato
We studied the ability of collagen vitrigel material to repair cartilage in vivo when used alone or with transforming growth factor-β (TGF-β). We measured the time course and quantity of TGF-β1 released from the collagen vitrigel in vitro to quantify the controlled release of TGF-β1. Over 14 days, 0.91 ng of TGF-β was released from the collagen vitrigel. Osteochondral defects were made in the femoral trochlear groove in 36 Japanese white rabbits, which were divided into three groups: untreated group (group A), collagen vitrigel-implanted group (group B), and TGF-β1-incorporated collagen vitrigel-implanted group (group C)...
October 12, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27689849/improved-human-endometrial-stem-cells-differentiation-into-functional-hepatocyte-like-cells-on-a-glycosaminoglycan-collagen-grafted-polyethersulfone-nanofibrous-scaffold
#12
Farzaneh Khademi, Jafar Ai, Masoud Soleimani, Javad Verdi, Seyed Mohammad Tavangar, Esmaeil Sadroddiny, Mohammad Massumi, Seyed Mahmoud Hashemi
Liver tissue engineering (TE) is rapidly emerging as an effective technique which combines engineering and biological processes to compensate for the shortage of damaged or destroyed liver tissues. We examined the viability, differentiation, and integration of hepatocyte-like cells on an electrospun polyethersulfone (PES) scaffold, derived from human endometrial stem cells (hEnSCs). Natural polymers were separately grafted on plasma-treated PES nanofibers, that is, collagen, heparan sulfate (HS) and collagen-HS...
September 30, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27678116/cellulose-synthesis-by-komagataeibacter-rhaeticus-strain-p-1463-isolated-from-kombucha
#13
Pavels Semjonovs, Maija Ruklisha, Longina Paegle, Madara Saka, Rita Treimane, Marite Skute, Linda Rozenberga, Laura Vikele, Martins Sabovics, Ilse Cleenwerck
Isolate B17 from Kombucha was estimated to be an efficient producer of bacterial cellulose (BC). The isolate was deposited under the number P 1463 and identified as Komagataeibacter rhaeticus by comparing a generated amplified fragment length polymorphism (AFLP™) DNA fingerprint against a reference database. Static cultivation of the K. rhaeticus strain P 1463 in Hestrin and Schramm (HS) medium resulted in 4.40 ± 0.22 g/L BC being produced, corresponding to a BC yield from glucose of 25.30 ± 1.78 %, when the inoculum was made with a modified HS medium containing 10 g/L glucose...
September 27, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27642037/the-development-and-effectiveness-of-an-ecological-momentary-intervention-to-increase-daily-fruit-and-vegetable-consumption-in-low-consuming-young-adults
#14
Kate L Brookie, Louise A Mainvil, Anitra C Carr, Margreet C M Vissers, Tamlin S Conner
OBJECTIVES: To develop and test the effectiveness of a mobile-phone based ecological momentary intervention (EMI) to increase fruit and vegetable (FV) consumption in low-consuming young adults. METHODS: A two-week randomised controlled trial of low-FV consuming young adults ages 18-25 years (n = 171) compared three conditions: ecological momentary intervention (EMI), fruit and vegetable intervention (FVI), and a diet-as-usual control (ANZCTRN12615000183583). Participants in the EMI condition were sent two targeted text messages a day for 13 days and were asked to increase daily FV consumption to at least five servings...
September 15, 2016: Appetite
https://www.readbyqxmd.com/read/27630205/apple-peel-polyphenols-a-key-player-in-the-prevention-and-treatment-of-experimental-inflammatory-bowel-disease
#15
Marie-Claude Denis, Denis Roy, Pantea Rahmani Yeganeh, Yves Desjardins, Thibault Varin, Nour Haddad, Devendra Amre, Alain Théophile Sané, Carole Garofalo, Alexandra Furtos, Natalie Patey, Edgard Delvin, Eric Tremblay, André Marette, Jean-François Beaulieu, Emile Levy
Diets rich in fruits and vegetables may reduce oxidative stress (OxS) and inflammation via several mechanisms. These beneficial effects may be due to their high polyphenol content. The aims of the present study are to evaluate the preventive and therapeutic aspects of polyphenols in dried apple peel powder (DAPP) on intestinal inflammation while elucidating the underlying mechanisms and clinical benefits. Induction of intestinal inflammation in mice was performed by oral administration of the inflammatory agent dextran sulfate sodium (DSS) at 2...
December 1, 2016: Clinical Science (1979-)
https://www.readbyqxmd.com/read/27622449/comparison-of-independent-and-dependent-culture-methods-for-the-detection-of-transient-bacteremia-in-diabetic-subjects-with-chronic-periodontitis
#16
Anna Carolina Ratto-Tespestini, Paula Juliana Pérez-Chaparro, Giuseppe Alexandre Romito, Luciene Cristina Figueiredo, Marcelo Faveri, Hilana Paula Carillo, Priscila Larcher, Magda Feres
INTRODUCTION: Oral-derived bacteremia may occur after several dental procedures and routine daily activities. Some conditions of the oral cavity may favor episodes of bacteremia. This would be the case of patients with diabetes mellitus and periodontitis, who exhibit exacerbated gingival inflammation and may be more prone to developing oral-derived bacteremia.  OBJECTIVE: To compare the effectiveness of an independent culture method (quantitative real-time PCR- qCR) and the most commonly used method (BacT-ALERT 3D®) for the diagnosis of bacteremia...
2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27611236/pati-patient-accessed-tailored-information-a-pilot-study-to-evaluate-the-effect-on-preoperative-breast-cancer-patients-of-information-delivered-via-a-mobile-application
#17
N M Foley, E P O'Connell, E A Lehane, V Livingstone, B Maher, S Kaimkhani, T Cil, N Relihan, M W Bennett, H P Redmond, M A Corrigan
OBJECTIVES: The information needs of cancer patients are highly variable. Literature suggests an improved ability to modulate personalised stress, increased patient involvement with decision making, greater satisfaction with treatment choices and reduced anxiety levels in cancer patients who have access to information. The aim of this project was to evaluate the effects of a mobile information application on anxiety levels of patients undergoing surgery for breast cancer. MATERIALS AND METHODS: An application was developed for use with Apple iPad containing information on basic breast cancer biology, different treatments used and surgical techniques...
December 2016: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/27603339/a-mobile-application-improves-therapy-adherence-rates-in-elderly-patients-undergoing-rehabilitation-a-crossover-design-study-comparing-documentation-via-ipad-with-paper-based-control
#18
Alexander Mertens, Christopher Brandl, Talya Miron-Shatz, Christopher Schlick, Till Neumann, Andreas Kribben, Sven Meister, Clarissa Jonas Diamantidis, Urs-Vito Albrecht, Peter Horn, Stefan Becker
Medication adherence is crucial for success in the management of patients with chronic conditions. This study analyzes whether a mobile application on a tablet aimed at supporting drug intake and vital sign parameter documentation affects adherence in elderly patients.Patients with coronary heart disease and no prior knowledge of tablet computers were recruited. They received a personal introduction to the mobile application Medication Plan, installed on an Apple iPad. The study was conducted using a crossover design with 3 sequences: initial phase, interventional phase (28 days of using the app system), and comparative phase (28 days of using a paper diary)...
September 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27603049/sol-gel-derived-bioactive-coating-on-zirconia-effect-on-flexural-strength-and-cell-proliferation
#19
Khalil Shahramian, Heidi Leminen, Ville Meretoja, Paula Linderbäck, Ilkka Kangasniemi, Lippo Lassila, Aous Abdulmajeed, Timo Närhi
The purpose of this study was to evaluate the effect of sol-gel derived bioactive coatings on the biaxial flexural strength and fibroblast proliferation of zirconia, aimed to be used as an implant abutment material. Yttrium stabilized zirconia disc-shaped specimens were cut, ground, sintered, and finally cleansed ultrasonically in each of acetone and ethanol for 5 minutes. Three experimental groups (n = 15) were fabricated, zirconia with sol-gel derived titania (TiO2 ) coating, zirconia with sol-gel derived zirconia (ZrO2 ) coating, and non-coated zirconia as a control...
September 7, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27591976/polyamines-in-brown-rice-vinegar-function-as-potent-attractants-for-the-spotted-wing-drosophila
#20
Naoki Akasaka, Haruka Higashikubo, Yuri Ishii, Hisao Sakoda, Shinsuke Fujiwara
Vinegar produced by acetic acid bacteria is used as an attractant for fruit flies. Apple cider vinegar (ACV) and brown rice vinegar (BRV) are used as lures to detect Drosophila suzukii (also known as the spotted wing drosophila [SWD], a newly emerging invasive pest of soft-skinned fruits) and to capture Drosophila melanogaster, respectively. In the present study, we evaluated the attractiveness of BRV and ACV to SWD in laboratory trapping experiments using an upturned microcentrifuge tube with a pipette tip as a trap...
August 31, 2016: Journal of Bioscience and Bioengineering
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