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https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#1
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328390/the-efficacy-of-telemedicine-supported-discharge-within-an-in-home-model-of-care
#2
Edwin P Greenup, Melissa McCusker, Boyd A Potts, Andrew Bryett
OBJECTIVE: To determine if mobile videoconferencing technology can facilitate the discharge of low-acuity patients receiving in-home care without compromising short-term health outcomes. METHODS: A 6-month trial commenced in July 2015 with 345 patients considered unsuited to Criteria Led Discharge (CLD) receiving in-home care included as participants. Nurses providing clinical support to patients in their homes were supplied with a tablet computer (Apple iPad) with Internet connectivity (Telstra 4G Network) and videoconferencing software (Cisco Jabber for Telepresence)...
February 22, 2017: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/28320409/can-an-incentive-based-intervention-increase-physical-activity-and-reduce-sitting-among-adults-the-achieve-active-choices-incentive-feasibility-study
#3
Kylie Ball, Ruth F Hunter, Jaimie-Lee Maple, Marj Moodie, Jo Salmon, Kok-Leong Ong, Lena D Stephens, Michelle Jackson, David Crawford
BACKGROUND: Despite recent interest in the potential of incentivisation as a strategy for motivating healthier behaviors, little remains known about the effectiveness of incentives in promoting physical activity and reducing sedentary behavior, and improving associated health outcomes. This pre-post-test design study investigated the feasibility, appeal and effects of providing non-financial incentives for promoting increased physical activity, reduced sedentary time, and reduced body mass index (BMI) and blood pressure among inactive middle-aged adults...
March 21, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28282248/what-will-it-take-to-end-the-hiv-aids-epidemic-linking-the-most-disenfranchised-into-care-through-outreach
#4
Cynthia A Gómez, Susana A Tat, Debra Allen, Danielle Gordon, Dennis Browe
The 2015 National HIV/AIDS Strategy renewed its goal of increasing access to care for people living with HIV/AIDS (PLWHA) and called for an increased focus on linkage to care efforts. As many PLWHA face multiple barriers to care and live on the margins of society, adoption of intensive outreach activities is necessary to engage the most disenfranchised PLWHA into care and to ultimately end the HIV epidemic. The Bay Area Network for Positive Health (BANPH), comprising 12+ agencies, established a network outreach model for our linkage-to-care project to engage the hardest-to-reach populations in the San Francisco Bay Area...
March 2017: AIDS Patient Care and STDs
https://www.readbyqxmd.com/read/28276202/safety-osseointegration-and-bone-ingrowth-analysis-of-pmma-based-porous-cement-on-animal-metaphyseal-bone-defect-model
#5
Bruno Cimatti, Mariana Avelino Dos Santos, Maria Sol Brassesco, Laura Tiemi Okano, Wendell Monteiro Barboza, Marcello Henrique Nogueira-Barbosa, Edgard Eduard Engel
Bone defects created after curettage of benign bone tumors are customarily filled with solid poly(methyl methacrylate) (PMMA) or other bone substitutes. In this study, we depicted a porous PMMA-based cement (produced by mixing sodium bicarbonate and citric acid) and evaluated the prospect of its clinic application. Cement samples were characterized by high-performance liquid chromatography (HPLC) coupled to mass spectrometry and its cytotoxicity evaluated in fibroblast cultures. Implantation in rabbits allowed the histologic analysis of bone, kidneys, and liver for toxicity and coagulation tests, and MRI images for hemostasis evaluation...
March 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28276115/a-novel-feedstuff-ensiling-of-cowpea-vigna-unguiculata-l-stover-and-apple-malus-domestica-borkh-mixtures-evaluation-of-the-nutritive-value-fermentation-quality-and-aerobic-stability
#6
Ederson Andrade, Alexandre Gonçalves, Ana Mendes-Ferreira, Valéria Silva, Victor Pinheiro, Miguel Rodrigues, Luis Ferreira
BACKGROUND: Agro-industrial by-products have low economical value as foods for human consumption however might represent a potential value as animal feedstuffs. This study evaluated a novel feedstuff, ensiled discarded apple (85%) and cowpea stover (15%) mixtures with two distinct ensiling periods (45 and 60 days) regarding the nutritive value, fermentation quality and aerobic stability. RESULTS: Generally, no differences (P > 0.05) were observed between ensiling periods for its nutritive value and fermentation characteristics...
March 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28263435/anti-inflammatory-coatings-of-hernia-repair-meshes-a-pilot-study
#7
Dmitry Gil, James Rex, William Cobb, Vladimir Reukov, Alexey Vertegel
The current prevalence of postoperative chronic pain from hernioplasty procedures employing polymer mesh is close to 30%. Most of the researchers agree that oxidative stress, resulting from the release of oxidants and enzymes during acute inflammatory response, is a key factor in the development of posthernioplasty complications. This results in both the decrease of the biomechanical properties and stiffening of the polymer fibers of the mesh, leading to chronic pain. Moreover, enhanced activity of inflammatory cells can lead to an excessive deposition of connective tissue around the implant...
March 6, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28257442/genotyping-by-sequencing-markers-facilitate-the-identification-of-quantitative-trait-loci-controlling-resistance-to-penicillium-expansum-in-malus-sieversii
#8
John L Norelli, Michael Wisniewski, Gennaro Fazio, Erik Burchard, Benjamin Gutierrez, Elena Levin, Samir Droby
Blue mold caused by Penicillium expansum is the most important postharvest disease of apple worldwide and results in significant financial losses. There are no defined sources of resistance to blue mold in domesticated apple. However, resistance has been described in wild Malus sieversii accessions, including plant introduction (PI)613981. The objective of the present study was to identify the genetic loci controlling resistance to blue mold in this accession. We describe the first quantitative trait loci (QTL) reported in the Rosaceae tribe Maleae conditioning resistance to P...
2017: PloS One
https://www.readbyqxmd.com/read/28251289/determination-of-volatile-compounds-in-cider-apple-juices-using-a-covalently-bonded-ionic-liquid-coating-as-the-stationary-phase-in-gas-chromatography
#9
Jairo Pello-Palma, Jaime González-Álvarez, María Dolores Gutiérrez-Álvarez, Enrique Dapena de la Fuente, Juan José Mangas-Alonso, Daniel Méndez-Sánchez, Vicente Gotor-Fernández, Pilar Arias-Abrodo
A chromatographic method for the separation of volatile compounds in Asturian cider apple juices has been developed. For this separation purpose, a monocationic imidazolium-based ionic liquid bearing a reactive terminal iodine atom was synthesized by a quaternization-anion exchange chemical sequence. Next, the gas chromatography (GC) stationary phase was prepared by covalently linking the imidazolium monolith to the reactive silanol groups of the inner capillary wall at 70 °C. This coated GC column exhibited good thermal stability (290 °C), as well as good efficiency (2000 plates/m) in the separation of volatile compounds from Asturian apple cider juices, and was characterized using the Abraham solvation parameter model...
March 1, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28240814/an-engineered-macroencapsulation-membrane-releasing-fty720-to-precondition-pancreatic-islet-transplantation
#10
Daniel T Bowers, Claire E Olingy, Preeti Chhabra, Linda Langman, Parker H Merrill, Ritu S Linhart, Michael L Tanes, Dan Lin, Kenneth L Brayman, Edward A Botchwey
Macroencapsulation is a powerful approach to increase the efficiency of extrahepatic pancreatic islet transplant. FTY720, a small molecule that activates signaling through sphingosine-1-phosphate receptors, is immunomodulatory and pro-angiogenic upon sustained delivery from biomaterials. While FTY720 (fingolimod, Gilenya) has been explored for organ transplantation, in the present work the effect of locally released FTY720 from novel nanofiber-based macroencapsulation membranes is explored for islet transplantation...
February 27, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28238909/apple-polysaccharide-inhibits-microbial-dysbiosis-and-chronic-inflammation-and-modulates-gut-permeability-in-hfd-fed-rats
#11
Sheng Wang, Qian Li, Yue Zang, Yang Zhao, Nan Liu, Yifei Wang, Xiaotao Xu, Li Liu, Qibing Mei
The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases. It has been well accepted that dysbiosis is the reflection of various chronic diseases. Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis. High-fat diet (HFD) fed rats were treated for 14 weeks with AP. The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed...
February 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28237958/study-protocol-for-a-pragmatic-randomised-controlled-trial-evaluating-efficacy-of-a-smoking-cessation-e-tabac-info-service-ee-tis-trial
#12
L Cambon, P Bergman, Al Le Faou, I Vincent, B Le Maitre, A Pasquereau, P Arwidson, D Thomas, F Alla
INTRODUCTION: A French national smoking cessation service, Tabac Info Service, has been developed to provide an adapted quitline and a web and mobile application involving personalised contacts (eg, questionnaires, advice, activities, messages) to support smoking cessation. This paper presents the study protocol of the evaluation of the application (e-intervention Tabac Info Service (e-TIS)). The primary objective is to assess the efficacy of e-TIS. The secondary objectives are to (1) describe efficacy variations with regard to users' characteristics, (2) analyse mechanisms and contextual conditions of e-TIS efficacy...
February 24, 2017: BMJ Open
https://www.readbyqxmd.com/read/28221974/evaluation-of-a-surface-sampling-method-for-recovery-of-human-noroviruses-prior-to-detection-using-reverse-transcription-quantitative-pcr
#13
Grace Tung-Thompson, Blanca I Escudero-Abarca, Janie Outlaw, Arnaud Ganee, Sylvanie Cassard, Claude Mabilat, Lee-Ann Jaykus
Human noroviruses are the most common cause of acute viral gastroenteritis, and the environmental persistence of these viruses contributes to their transmissibility. Environmental sampling is thus an important tool for investigating norovirus outbreaks and for assessing the effectiveness of cleaning and decontamination regimens. The purpose of this study was to evaluate a sampling material (wipes) for their efficacy at recovering human norovirus from hard surfaces and foods. Dilutions of a human norovirus GII...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28196336/regression-of-atherosclerosis-with-apple-procyanidins-by-activating-the-atp-binding-cassette-subfamily-a-member-1-in-a-rabbit-model
#14
Liang Wang, Toshio Fumoto, Saeko Masumoto, Toshihiko Shoji, Tomisato Miura, Masato Naraoka, Naoya Matsuda, Tadaatsu Imaizumi, Hiroki Ohkuma
BACKGROUND AND AIMS: Apple polyphenol contains abundant procyanidins, which have been associated with an anti-atherosclerosis and cholesterol-lowering effect. The aim of this study was to investigate whether apple procyanidins (APCs) feature therapeutic efficacy in terms of regressing atherosclerosis and whether this efficacy is due to mechanisms other than a cholesterol-lowering effect. METHODS: After eight weeks on an atherogenic diet, rabbits were given a normal diet for another eight weeks to normalize the increased serum lipids level...
March 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28194717/metal-chelate-immobilization-of-lipase-onto-polyethylenimine-coated-mcm-41-for-apple-flavor-synthesis
#15
Armin Sadighi, Seyed Farshad Motevalizadeh, Morteza Hosseini, Ali Ramazani, Lena Gorgannezhad, Hamid Nadri, Behnaz Deiham, Mohammad Reza Ganjali, Abbas Shafiee, Mohammad Ali Faramarzi, Mehdi Khoobi
An enzyme immobilized on a mesoporous silica nanoparticle can serve as a multiple catalyst for the synthesis of industrially useful chemicals. In this work, MCM-41 nanoparticles were coated with polyethylenimine (MCM-41@PEI) and further modified by chelation of divalent metal ions (M = Co(2+), Cu(2+), or Pd(2+)) to produce metal-chelated silica nanoparticles (MCM-41@PEI-M). Thermomyces lanuginosa lipase (TLL) was immobilized onto MCM-41, MCM-41@PEI, and MCM-41@PEI-M by physical adsorption. Maximum immobilization yield and efficiency of 75 ± 3...
February 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28140510/improving-risk-assessment-of-color-additives-in-medical-device-polymers
#16
Vaishnavi Chandrasekar, Dustin W Janes, Christopher Forrey, David M Saylor, Akhil Bajaj, Timothy V Duncan, Jiwen Zheng, Kausar B Riaz Ahmed, Brendan J Casey
Many polymeric medical device materials contain color additives which could lead to adverse health effects. The potential health risk of color additives may be assessed by comparing the amount of color additive released over time to levels deemed to be safe based on available toxicity data. We propose a conservative model for exposure that requires only the diffusion coefficient of the additive in the polymer matrix, D, to be specified. The model is applied here using a model polymer (poly(ether-block-amide), PEBAX 2533) and color additive (quinizarin blue) system...
January 31, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28130877/mimicking-physiological-flow-conditions-to-study-alterations-of-bioactive-glass-surfaces-in-vitro
#17
Miriam Höner, Frederik Böke, Michael Weber, Horst Fischer
Bioactive glasses form a strong bond with surrounding tissue and slowly degrade when implanted in vivo, stimulating the host bone to regenerate itself. We investigated the behaviour of microstructured bioactive glass surfaces (13-93) in an SBF reactor, which mimics physiological flow conditions. The structures were developed to potentially influence cell-biological long term processes such as osteogenic differentiation. It is therefore important that the structures withstand a certain time in SBF or body fluids...
January 27, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28130836/optimized-performance-of-the-integrated-hepatic-cell-loaded-cryogel-based-bioreactor-with-intermittent-perfusion-of-acute-liver-failure-plasma
#18
Apeksha Damania, Anupam Kumar, Shiv K Sarin, Ashok Kumar
Acute liver failure (ALF) plasma has cytotoxic effects on the cell-loaded bioreactor in bioartificial liver support systems due to the presence of innumerable hepatotoxic compounds that adversely affect the morphology and functionality of the cells. We have designed a hybrid bioreactor that integrates a hepatic cell-loaded cryogel disc and an activated carbon cloth in one compact unit, with potential application as a bioartificial liver support. In this article, we assess the performance of this integrated hybrid cryogel-based bioreactor in a perfusion-based culture system and analyze its functionality and longevity in the presence of intermittent exposure to ALF plasma...
January 27, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28103422/vitamin-supplementation
#19
EDITORIAL
P B Persson, A B Persson
No abstract text is available yet for this article.
March 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28101467/some-qualitative-and-rheological-properties-of-virgin-olive-oil-apple-vinegar-salad-dressing-stabilized-with-xanthan-gum
#20
Solmaz Abedinzadeh, Mohammadali Torbati, Sodeif Azadmard-Damirchi
Purpose: Lipid oxidation and rheological properties are the main qualitative parameters determined in food emulsions. Salad dressings are food emulsions important in our daily diet, but conventional salad dressings have high amounts of cholesterol and saturated fatty acids because of egg yolk in their formulations. There are many studies on the modification of salad dressing formulations to replace egg yolk and saturated fats. The present study describes new formulation of salad dressing with olive oil and apple vinegar to produce a functional food product...
December 2016: Advanced Pharmaceutical Bulletin
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