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an Apple a day

Sanaz Jarolmasjed, Lav R Khot, Sindhuja Sankaran
Bitter pit is one of the most important disorders in apples. Some of the fresh market apple varieties are susceptible to bitter pit disorder. In this study, visible⁻near-infrared spectrometry-based reflectance spectral data (350⁻2500 nm) were acquired from 2014, 2015 and 2016 harvest produce after 63 days of storage at 5 °C. Selected spectral features from 2014 season were used to classify the healthy and bitter pit samples from three years. In addition, these spectral features were also validated using hyperspectral imagery data collected on 2016 harvest produce after storage in a commercial storage facility for 5 months...
May 15, 2018: Sensors
M I Serqushkina, A N Khudyakov, T V Polezhaeva, O M Bezmeltseva
BACKGROUND: Pectins have a large number of active -OH and -COOH groups (3), suggesting the presence of this group of substances specific cryoprotective effect. OBJECTIVE: This research is aimed at studying the ability of pectins (zosteran, apple pectin АU-701, E-440) to change the freezing temperature of glycerol, which can increase the effect in the cryopreservation of nuclear cells. METHODS: Using the cryoscopic method we studied the ability of pectins (zosteran, apple pectin AU-701, E-440) to change the freezing temperature of glycerol...
November 2017: Cryo Letters
Jing Guo, Minmin Li, Yongguo Liu, Fengzhong Wang, Zhiqiang Kong, Yufeng Sun, Jia Lu, Nuo Jin, Yatao Huang, Jiameng Liu, Frédéric Francis, Bei Fan
Ultra-performance convergence chromatography is an environmentally-friendly analytical method that uses dramatically reduced amounts of organic solvents. In addition, a robust and highly sensitive chiral separation method was developed for the novel chiral acaricide cyflumetofen by using ultra-performance convergence chromatography coupled with tandem mass spectrometry, which shows that stereoisomer recoveries determined for various apple parts ranged from 78.3% to 119.9%, with the relative standard deviations being lower than 14...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Maria Elena Alemán-Domínguez, Elena Giusto, Zaida Ortega, Maryam Tamaddon, Antonio Nizardo Benítez, Chaozong Liu
Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties. Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering. Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix...
May 2, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Larissa Stebounova, Scott M Ebert, Logan T Murry, Christopher M Adams, Daryl J Murry
Ultra-performance liquid chromatography (UPLC) interfaced with atmospheric pressure chemical ionization mass-spectrometry was used to separate and quantify ursolic acid (UA) and oleanolic acid (OA) in human plasma. UA and OA were extracted from 0.5 mL human plasma using supported liquid extraction and separated utilizing an Acquity UPLC HSS column. The method has been validated for both UA and OA quantitation with a limit of detection of 0.5 ng/mL. The UPLC separations are carried out with isocratic elution with methanol and 5 mM ammonium acetate in water (85:15) as a mobile phase at a flow rate of 0...
April 26, 2018: Journal of Chromatographic Science
Michael Koerner, Eliza Fredette, Trudy A Watt, Peter Lloyd Jones
No abstract text is available yet for this article.
April 17, 2018: Annals of Internal Medicine
Pantea Rahmani Yeganeh, Jade Leahy, Schohraya Spahis, Natalie Patey, Yves Desjardins, Denis Roy, Edgard Delvin, Carole Garofalo, Jean-Philippe Leduc-Gaudet, David St-Pierre, Jean-François Beaulieu, André Marette, Gilles Gouspillou, Emile Levy
Inflammatory bowel diseases (IBDs) are multifaceted and relapsing immune disorders, which necessitate long-term dependence on powerful drugs. As the use of natural product-based therapies has emerged as a promising intervention, the present study aimed to further characterize dried apple peel powder (DAPP) mechanisms of action and evaluate the preventive and curative effects of DAPP on mitochondrial functions in a murine model. Induction of intestinal inflammation in mice is performed by oral administration of the dextran sodium sulfate (DSS) at 2...
March 21, 2018: Journal of Nutritional Biochemistry
Andrew T Wood, Dominique Everett, Sanjay Kumar, Manoj K Mishra, Vinoy Thomas
In the area of biomaterials, fibers not only offer increased mechanical response, but also serve as an extracellular matrix mimicking morphology to direct cellular attachment and proliferation. While biologically similar in morphology, soft, and flexible hydrogel materials have low mechanical properties. For applications in tissue engineering, the lack of directional cues and attachment regions within the biogels is undesired as cells require a guide for adequate attachment and organized proliferation. In this work, we have investigated the role of poly(lactic acid) (PLA) fiber length and concentration as a reinforcement phase in a gelatin hydrogel matrix and the resultant mechanical and cellular responses...
April 15, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Nana Sun, Yuan Ding, Zhexuan Tao, Hongjie You, Xiude Hua, Minghua Wang
An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity (△I) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid...
August 15, 2018: Food Chemistry
Hanie Yousefi, M Monsur Ali, Hsuan-Ming Su, Carlos D M Filipe, Tohid F Didar
Here, we report the development of a transparent, durable, and flexible sensing surface that generates a fluorescence signal in the presence of a specific target bacterium. This material can be used in packaging, and it is capable of monitoring microbial contamination in various types of food products in real time without having to remove the sample or the sensor from the package. The sensor was fabricated by covalently attaching picoliter-sized microarrays of an E. coli-specific RNA-cleaving fluorogenic DNAzyme probe (RFD-EC1) to a thin, flexible, and transparent cyclo-olefin polymer (COP) film...
April 6, 2018: ACS Nano
Jared A Jaffey, Robert C Backus, Kaylyn M McDaniel, Amy E DeClue
Hypovitaminosis D has been extensively documented in critically ill humans. However, whether or not critically ill dogs have alterations in vitamin D concentrations remains unconfirmed. The primary aims of our study were to compare serum 25-hydroxycholecalciferol [25(OH)D] concentrations in critically ill dogs with healthy control dogs, determine the prognostic utility of serum 25(OH)D concentration as a biomarker in critically ill dogs, and to assess if serum 25(OH)D concentrations in critically ill dogs are associated with length of stay in the intensive care unit or illness severity...
2018: PloS One
Jiho Lee, Eunhye Kim, Yongho Shin, Junghak Lee, Jonghwa Lee, Joon-Kwan Moon, Hoon Choi, Wolfgang Maasfeld, Jeong-Han Kim
This study examined dermal and inhalation exposure of agricultural operators to kresoxim-methyl during pesticide mixing/loading and speed sprayer application (10 replicates, each of 3000 L of spray suspension) in an apple orchard and performed risk assessment. For the whole body dosimetry (WBD) exposure protocol, outer clothing, inner clothing, gauze, and nitrile gloves were examined to measure dermal exposure. In contrast, an IOM (institute of occupational medicine) sampler with a glass fiber filter was used to measure inhalation exposure...
July 15, 2018: Ecotoxicology and Environmental Safety
Chao Lei, Sheng Fan, Ke Li, Yuan Meng, Jiangping Mao, Mingyu Han, Caiping Zhao, Lu Bao, Dong Zhang
Adventitious root (AR) formation, which is controlled by endogenous and environmental factors, is indispensable for vegetative asexual propagation. However, comprehensive proteomic data on AR formation are still lacking. The aim of this work was to study indole-3-butyric acid (IBA)-induced AR formation in the dwarf apple rootstock 'T337'. In this study, the effect of IBA on AR formation was analysed. Subsequent to treatment with IBA, both the rooting rate and root length of 'T337' increased significantly. An assessment of hormone levels in basal stem cuttings suggested that auxin, abscisic acid, and brassinolide were higher in basal stem cuttings that received the exogenous IBA application; while zeatin riboside, gibberellins, and jasmonic acid were lower than non-treated basal stem cuttings...
February 27, 2018: International Journal of Molecular Sciences
Y Yusha'u, U A Muhammad, M Nze, J M Egwuma, O J Igomu, M Abdulkadir
Flavonoids have been demonstrated to possess an anti-depressant effect and less adverse effects than tricyclic anti-depressants. For this reason, flavonoids in natural products have attracted growing attention. Rutin is a glycoside flavonoid which belongs to an important class of flavonoids, abundantly found in plants, such as buckwheat seeds, asparagus, red pepper, apples, citrus fruits and leaves of many herbs such as rosemary, dandelion or sage, and black and green tea.  It is a vital nutritional component of food stuff...
December 30, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
Bruna Nunes Teixeira, Paola Aprile, Roberta H Mendonça, Daniel J Kelly, Rossana Mara da Silva Moreira Thiré
The majority of synthetic polymers used in 3 D printing are not designed to promote specific cellular interactions and hence possess limited bioactivity. Most of the strategies proposed to overcome this limitation demand multiple and expensive processing steps. This study aimed to evaluate the surface modification of 3D-printed poly(lactic acid) (PLA) scaffolds with polydopamine (PDA) coating as an alternative strategy to enhance their bioactivity and to facilitate the immobilization of type I collagen (COL I) onto the implant surface...
February 26, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Benedicte Jalbert, Nguyen Toan Tran, Stephan von Düring, Pierre-Alexandre Poletti, Ian Fournier, Catherine Hafner, Celestine Dubost, Laurent Gétaz, Hans Wolff
BACKGROUND: Drug dealers and drug users resort to body stuffing to hastily conceal illicit drugs by ingesting their drug packets. This practice represents a medical challenge because rupture of the often insecure packaging can be toxic and even lethal. In an emergency setting, official guidelines are needed to help the medical team decide on the proper treatment. A preliminary observation period is generally accepted but its duration varies from hours to eventual packet expulsion. CASE PRESENTATION: This case involves a 20-year-old white man in detention who claimed to have ingested one cocaine packet wrapped in plastic food-wrap and a condom in anticipation of an impending cell search...
February 11, 2018: Journal of Medical Case Reports
(no author information available yet)
A randomised study in young subjects of the effects of eating extra fruit or nuts on periodontal inflammation.
February 9, 2018: British Dental Journal
Ioannis K Karoussis, Kyriaki Kyriakidou, Joseph Papaparaskevas, Ioannis A Vrotsos, Mara Simopoulou, Georgios A Kotsakis
Bacterial peri-implant biofilms, and the chemotherapeutics for their removal alter titanium surface cytocompatibility. In this study we aimed to assess the adjunctive use of an osteostimulative biomaterial utilizing a peri-implantitis model under the hypothesis that it will increase cell migration towards treated titanium surfaces. Acid-etched titanium surfaces were inoculated with a multi-species biofilm model and treated with 1.5% NaOCl in a previously characterized in vitro peri-implantitis model. Cell migration of MG63 cells towards the treated titanium surface (CTRL) was significantly reduced following inoculation with biofilm and chemotherapeutic treatment as compared to sterile controls...
February 5, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
K Murray, P Moyer, F Wu, J B Goyette, K Warriner
This study evaluated the efficacy of using sequential forced air ozone followed by an advanced oxidative process (AOP) treatment to inactivate Listeria monocytogenes on and within Empire apples. The forced air ozone treatment consisted of a reactor that introduced ozone (6 g/h) into an airstream that flowed through an apple bed (ca. 30 cm in depth). Before treatment, the apples were conditioned at 4°C to ensure that condensate had formed before the apples were transferred to the reactor. The condensate ensured sufficient relative humidity to enhance the antimicrobial action of ozone...
March 2018: Journal of Food Protection
Shuang Guo, Yuehua Wang, Shurui Chou, Huijun Cui, Dongnan Li, Bin Li
In the present study, the DPPH and ABTS+ radical scavenging activity of eight types of apples decreased (P < 0.05) during the 70-day storage at 4°C. The Fushi (F2) apples from Xin Jiang showed the highest radical scavenging activity. For in vivo study, 40 male Kunming mice (body weight 20-25 g) were selected and randomly assigned to four groups (10 mice per group). The F2 groups (F2S, F2 + sterile saline and F2L, F2 + lipopolysaccharide) were administered with 0.3 mL F2 filtrate via gastric intubation daily for 28 days...
2018: PloS One
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