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https://www.readbyqxmd.com/read/27917188/crispr-cas9-tool-for-qualitative-and-quantitative-plant-genome-editing
#1
REVIEW
Ali Noman, Muhammad Aqeel, Shuilin He
Recent developments in genome editing techniques have aroused substantial excitement among agricultural scientists. These techniques offer new opportunities for developing improved plant lines with addition of important traits or removal of undesirable traits. Increased adoption of genome editing has been geared by swiftly developing Clustered regularly interspaced short palindromic repeats (CRISPR). This is appearing as driving force for innovative utilization in diverse branches of plant biology. CRISPR-Cas9 mediated genome editing is being used for rapid, easy and efficient alteration of genes among diverse plant species...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27915147/highly-sensitive-optical-interferometric-technique-reveals-stress-dependent-instantaneous-nanometric-growth-fluctuations-of-chinese-chive-leaf-under-heavy-metal-stress
#2
Kokge Thilini Kanchana Muthumali DeSilva, Uma Maheswari Rajagopalan, Hirofumi Kadono
Plant growth apart from being a complex and highly dynamic is dependent on its immediate environment. Leaf expansion measurements using Statistical Interferometry Technique, a sensitive interferometric technique at nanometric accuracy and at sub-second levels revealed the presence of characteristic nanometric intrinsic fluctuations [Plant Biotechnology 31, 195 (2014)]. In this paper, we demonstrate that the nanometric intrinsic fluctuations are sensitive enough that they change under exposure of heavy metals, essential micronutrient zinc and non-essential element cadmium, at relatively low concentrations in the leaves of Chinese chive (Allium tuberosum)...
December 1, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27912886/medicinal-plants-and-natural-molecules-with-in-vitro-and-in-vivo-activity-against-rotavirus-a-systematic-review
#3
REVIEW
Gopalsamy Rajiv Gandhi, Paula Gurgel Barreto, Bruno Dos Santos Lima, Jullyana de Souza Siqueira Quintans, Adriano Antunes de Souza Araújo, Narendra Narain, Lucindo Jose Quintans-Júnior, Ricardo Queiroz Gurgel
BACKGROUND: Rotaviruses can cause life-threatening health disorders, such as severe dehydrating gastroenteritis and diarrhea in children. Vaccination is the main preventive strategy to reduce rotavirus diarrhea and the severity of episodes, but vaccines are not fully effective and new episodes may occur, even in vaccinated children. The WHO recommends oral rehydration therapy and zinc supplementation for rotavirus-induced diarrhea management. There is little preclinical evidence to support the use of phytotherapeutics in the management of rotaviral infections...
December 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27888434/a-novel-heavy-metal-atpase-peptide-from-prosopis-juliflora-is-involved-in-metal-uptake-in-yeast-and-tobacco
#4
Nisha S Keeran, G Ganesan, Ajay K Parida
Heavy metal pollution of agricultural soils is one of the most severe ecological problems in the world. Prosopis juliflora, a phreatophytic tree species, grows well in heavy metal laden industrial sites and is known to accumulate heavy metals. Heavy Metal ATPases (HMAs) are ATP driven heavy metal pumps that translocate heavy metals across biological membranes thus helping the plant in heavy metal tolerance and phytoremediation. In the present study we have isolated and characterized a novel 28.9 kDa heavy metal ATPase peptide (PjHMT) from P...
November 25, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27880006/impairing-both-hma4-homeologs-is-required-for-cadmium-reduction-in-tobacco
#5
Verena Liedschulte, Hélène Laparra, James Nicolas Duncan Battey, Joanne Deborah Schwaar, Hervé Broye, Régis Mark, Markus Klein, Simon Goepfert, Lucien Bovet
In tobacco, the heavy metal P1B-ATPases HMA4.1 and HMA4.2 function in root-to-shoot zinc and cadmium transport. We present greenhouse and field data that dissect the possibilities to impact the two homeologous genes in order to define the best strategy for leaf cadmium reduction. In a first step, both genes were silenced using an RNAi approach leading to >90% reduction of leaf cadmium content. To modulate HMA4 function more precisely, mutant HMA4.1 and HMA4.2 alleles of a TILLING population were combined...
November 23, 2016: Plant, Cell & Environment
https://www.readbyqxmd.com/read/27877177/comparative-transcriptomic-analyses-of-differentially-expressed-genes-in-transgenic-melatonin-biosynthesis-ovine-hiomt-gene-in-switchgrass
#6
Shan Yuan, Cong Guan, Sijia Liu, Yanhua Huang, Danyang Tian, Xin Cui, Yunwei Zhang, Fuyu Yang
Melatonin serves pleiotropic functions in prompting plant growth and resistance to various stresses. The accurate biosynthetic pathway of melatonin remains elusive in plant species, while the N-acetyltransferase and O-methyltransferase were considered to be the last two key enzymes during its biosynthesis. To investigate the biosynthesis and metabolic pathway of melatonin in plants, the RNA-seq profile of overexpression of the ovine HIOMT was analyzed and compared with the previous transcriptome of transgenic oAANAT gene in switchgrass, a model plant for cellulosic ethanol production...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27868376/n-terminal-region-of-cysteine-rich-protein-crp-in-carlaviruses-is-involved-in-the-determination-of-symptom-types
#7
Naoko Fujita, Ken Komatsu, Yu Ayukawa, Yuki Matsuo, Masayoshi Hashimoto, Osamu Netsu, Tohru Teraoka, Yasuyuki Yamaji, Shigetou Namba, Tsutomu Arie
Plant viruses in the genus Carlavirus include more than 65 members. Plants infected with carlaviruses exhibit various symptoms including leaf malformation and plant stunting. Cysteine-rich protein (CRP) encoded by carlaviruses has been reported to be a pathogenicity determinant. Carlavirus CRPs contain two motifs in their central part: nuclear localization signal (NLS) and zinc finger motif (ZF). Besides these two conserved motifs, carlavirus CRPs possess highly diverged N-terminal 34 amino acid residues with unknown function...
November 21, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27864737/significance-of-diazotrophic-plant-growth-promoting-herbaspirillum-sp-gw103-on-phytoextraction-of-pband-zn-by-zea-mays-l
#8
Loganathan Praburaman, Sung-Hee Park, Min Cho, Kui-Jae Lee, Jeong-Ae Ko, Sang-Sub Han, Sang-Hyun Lee, Seralathan Kamala-Kannan, Byung-Taek Oh
Microbe-assisted phytoremediation has been considered a promising measure for the remediation of heavy metal-polluted soil. The aim of this study was to assess the effect of diazotrophic plant growth-promoting Herbaspirillum sp. GW103 on growth and lead (Pb) and zinc (Zn) accumulation in Zea mays L. The strain GW103 exhibited plant growth-promoting traits such as indole-3-acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylic deaminase. Treatment of Z. mays L. plants with GW103 significantly increased 19, 31, and 52% of plant biomass and 10, 50, and 126% of chlorophyll a contents in Pb, Zn, and Pb + Zn-amended soils, respectively...
November 18, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27858514/involvement-of-sth7-in-light-adapted-development-in-arabidopsis-thaliana-promoted-by-both-strigolactone-and-karrikin
#9
Jutiporn Thussagunpanit, Yuko Nagai, Miyu Nagae, Kiyoshi Mashiguchi, Nobutaka Mitsuda, Masaru Ohme-Takagi, Takeshi Nakano, Hidemitsu Nakamura, Tadao Asami
Strigolactones (SLs) and karrikins (KARs) regulate photomorphogenesis. GR24, a synthetic SL and KAR1, a KAR, inhibit the hypocotyl elongation of Arabidopsis thaliana in a weak light. GR24 and KAR1 up-regulate the expression of STH7, encoding a transcription factor belonging to the double B-box zinc finger subfamily. In this study, we used STH7-overexpressing (STH7ox) lines and functionally defective STH7 (STH7-SRDX) mutants to investigate roles of SLs and KARs in photomorphogenesis of Arabidopsis. Hypocotyl elongation of STH7-SRDX mutants was less sensitive to both GR24 and KAR1 treatment than that of wild-type Arabidopsis under weak light conditions...
November 18, 2016: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27854061/metal-resistant-rhizobacteria-isolates-improve-mucuna-deeringiana-phytoextraction-capacity-in-multi-metal-contaminated-soils-from-a-gold-mining-area
#10
Cácio Luiz Boechat, Patricia Giovanella, Magno Batista Amorim, Enilson Luiz Saccol de Sá, Flávio Anastácio de Oliveira Camargo
Phytoremediation consists of biological techniques for heavy metal remediation, which include exploring the genetic package of vegetable species to remove heavy metals from the environment. The goals of this study were to investigate heavy metal and bioaugmentation effects on growth and nutrient uptake by Mucuna deeringiana; to determine the metal translocation factor and bioconcentration factor and provide insight for using native bacteria to enhance heavy metal accumulation. The experiment was conducted under greenhouse conditions using a 2 × 4 factorial scheme with highly and slightly contaminated soil samples and inoculating M...
November 16, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27846808/pinpointing-genes-underlying-annual-perennial-transitions-with-comparative-genomics
#11
Andrew J Heidel, Christiane Kiefer, George Coupland, Laura E Rose
BACKGROUND: Transitions between perennial and an annual life history occur often in plant lineages, but the genes that control whether a plant is an annual or perennial are largely unknown. To identify genes that confer differences between annuals and perennials we compared the gene content of four pairs of sister lineages (Arabidopsis thaliana/Arabidopsis lyrata, Arabis montbretiana/Arabis alpina, Arabis verna/Aubrieta parviflora and Draba nemorosa/Draba hispanica) in the Brassicaceae in which each pair contains one annual and one perennial, plus one extra annual species (Capsella rubella)...
November 15, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27837364/translocation-of-metal-ions-from-soil-to-tobacco-roots-and-their-concentration-in-the-plant-parts
#12
Cleber Pinto da Silva, Thiago E de Almeida, Rosimara Zittel, Tatiana R de Oliveira Stremel, Cinthia E Domingues, Januário Kordiak, Sandro Xavier de Campos
This paper presents a study on the translocation factors (TFs) and bioconcentration factors (BCFs) of copper (Cu), manganese (Mn), zinc (Zn), cobalt (Co), chromium (Cr), cadmium (Cd), lead (Pb), iron (Fe), nickel (Ni), and arsenic (As) ions in roots, stems, and leaves of tobacco. The results revealed that during the tobacco growth, the roots are able to increase the sensitiveness of the physiological control, reducing the translocation of the metals Ni (0.38) and Pb (0.48) to the leaves. Cd and Zn presented factors TF and BCF >1 in the three tissues under analysis, which indicates the high potential for transportation and accumulation of these metals in all plant tissues...
December 0: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27836340/short-term-low-severity-spring-grassland-fire-impacts-on-soil-extractable-elements-and-soil-ratios-in-lithuania
#13
Paulo Pereira, Artemi Cerda, Deborah Martin, Xavier Úbeda, Daniel Depellegrin, Agata Novara, Juan F Martínez-Murillo, Eric C Brevik, Oleksandr Menshov, Jesus Rodrigo Comino, Jessica Miesel
Spring grassland fires are common in boreal areas as a consequence of slash and burn agriculture used to remove dry grass to increase soil nutrient properties and crop production. However, few works have investigated fire impacts on these grassland ecosystems, especially in the immediate period after the fire. The objective of this work was to study the short-term impacts of a spring grassland fire in Lithuania. Four days after the fire we established a 400m(2) sampling grid within the burned area and in an adjacent unburned area with the same topographical, hydrological and pedological characteristics...
November 8, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27833841/plant-growth-promotion-and-biofortification-of-chickpea-and-pigeonpea-through-inoculation-of-biocontrol-potential-bacteria-isolated-from-organic-soils
#14
Subramaniam Gopalakrishnan, Srinivas Vadlamudi, Srinivasan Samineni, C V Sameer Kumar
Seven strains of bacteria [Pseudomonas plecoglossicida SRI-156, Brevibacterium antiquum SRI-158, Bacillus altitudinis SRI-178, Enterobacter ludwigii SRI-211, E. ludwigii SRI-229, Acinetobacter tandoii SRI-305 and Pseudomonas monteilii SRI-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (PGP) in rice] were evaluated for their PGP and biofortification traits in chickpea and pigeonpea under field conditions. When treated on seed, the seven selected bacteria significantly enhanced the shoot height and root length of both chickpea and pigeonpea over the un-inoculated control...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27831816/genome-wide-analysis-of-the-dna-binding-with-one-zinc-finger-dof-transcription-factor-family-in-bananas
#15
Chen Dong, Huigang Hu, Jianghui Xie
DNA-binding with one finger (Dof) domain proteins are a multigene family of plant-specific transcription factors involved in numerous aspects of plant growth and development. In this study, we report a genome-wide search for Musa acuminata Dof (MaDof) genes and their expression profiles at different developmental stages and in response to various abiotic stresses. In addition, a complete overview of the Dof gene family in bananas is presented, including the gene structures, chromosomal locations, cis-regulatory elements, conserved protein domains, and phylogenetic inferences...
September 14, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27823905/bioassessment-of-heavy-metals-in-the-surface-soil-layer-of-an-opencast-mine-aimed-for-its-rehabilitation
#16
Vlado Ličina, Milica Fotirić Akšić, Zorica Tomić, Ivana Trajković, Svetlana Antić Mladenović, Milena Marjanović, Jörg Rinklebe
The contemporary reclamation method in an opencast coal mine closure comprises the use of the preserved surface soil layer (SSL) before mining, and can be directly returned to the areas being rehabilitated. The present study emphasizes a risk in the use of such a SSL in mine rehabilitation due to the possible excessive amount of heavy metals which usually derives from a metal-rich sediment or fluvial character of overburden material. This indication was approved by the bioassessment of cadmium (Cd), chromium (Cr), cobalt (Co), copper (Cu), lead (Pb), nickel (Ni), and zinc (Zn) in root and aerial parts of maize (Zea mays), alfalfa (Medicago sativa), sweet clover (Melilotus officinalis), wheat (Triticum aestivum), barley (Hordeum sativum), white clover (Trifolium repens), pasture (Poales sp...
November 4, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27822736/cisgenesis-and-intragenesis-in-microalgae-promising-advancements-towards-sustainable-metabolites-production
#17
REVIEW
Janeth I Galarza, Ninoska Delgado, Vitalia Henríquez
Microalgae are an economically important source of biomolecules and metabolites that can be exploited as feed, nutraceuticals and, potentially, as biofuels, among other biotechnological applications. Microalgae biotechnology involves both culture and metabolic pathways manipulation to obtain high-value products, such as omega-3 fatty acids and carotenoids. However, the introduction of genes and/or foreign regulatory sequences has caused public concern about the effect of genetically modified microalgae to achieve greater secondary metabolite accumulations...
November 7, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27822692/genetic-and-biochemical-characterization-of-rhizobacterial-strains-and-their-potential-use-in-combination-with-chelants-for-assisted-phytoremediation
#18
Cicatelli Angela, Guarino Francesco, Baldan Enrico, Castiglione Stefano
Copper and zinc are essential micronutrients in plants but, at high concentrations, they are toxic. Assisted phytoremediation is an emerging "green" technology that aims to improve the efficiency of tolerant species to remove metals from soils through the use of chelants or microorganisms. Rhizobacteria can promote plant growth and tolerance and also affect the mobility, bioavailability, and complexation of metals. A pot experiment was conducted to evaluate the phytoremediation effectiveness of sunflowers cultivated in a Cu- and Zn-spiked soil, in the presence or absence of bacterial consortium and/or chelants...
November 7, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27809771/proteomic-analysis-of-maize-grain-development-using-itraq-reveals-temporal-programs-of-diverse-metabolic-processes
#19
Tao Yu, Geng Li, Shuting Dong, Peng Liu, Jiwang Zhang, Bin Zhao
BACKGROUND: Grain development in maize is an essential process in the plant's life cycle and is vital for use of the plant as a crop for animals and humans. However, little is known regarding the protein regulatory networks that control grain development. Here, isobaric tag for relative and absolute quantification (iTRAQ) technology was used to analyze temporal changes in protein expression during maize grain development. RESULTS: Maize grain proteins and changes in protein expression at eight developmental stages from 3 to 50 d after pollination (DAP) were performed using iTRAQ-based proteomics...
November 4, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27808449/additive-toxicity-of-zinc-and-arsenate-on-barley-hordeum-vulgare-root-elongation
#20
Georgina Guzmán-Rangel, Liske Versieren, Hao Qiu, Erik Smolders
Zinc (Zn) and arsenic (As) are typically present as mixed contaminants in mining-impacted areas, however, their joined effects have rarely been evaluated. The present study was set-up to test if Zn(2+) and H2 AsO4(-) (further: As) mixture toxicity to plants is additive or if interactions occur. Barley (Hordeum vulgare) root elongation was measured in resin buffered nutrient solutions. The design included ranges of single element concentrations and combinations at three different Ca(2+) concentrations (0.5, 2...
November 3, 2016: Environmental Toxicology and Chemistry
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