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https://www.readbyqxmd.com/read/28440060/a-practical-approach-in-bioreactor-scale-up-and-process-transfer-using-a-combination-of-constant-p-v-and-vvm-as-the-criterion
#1
Sen Xu, Linda Hoshan, Rubin Jiang, Balrina Gupta, Eric Brodean, Kristin O'Neill, T Craig Seamans, John Bowers, Hao Chen
Bioreactor scale-up is a critical step in the production of therapeutic proteins such as monoclonal antibodies (MAbs). With the scale-up criterion such as similar power input per volume or O2 volumetric mass transfer coefficient (kL a), adequate oxygen supply and cell growth can be largely achieved. However, CO2 stripping in the growth phase is often inadequate. This could cascade down to increased base addition and osmolality, as well as residual lactate increase and compromised production and product quality...
April 25, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28437707/effect-of-pco2-on-direct-flue-gas-mineral-carbonation-at-pilot-scale
#2
Ikbel Mouedhen, Nassima Kemache, Louis-César Pasquier, Emmanuelle Cecchi, Jean-François Blais, Guy Mercier
Concerns about global warming phenomena induced the development of research about the control of anthropogenic greenhouse gases emissions. The current work studies on the scaling up of aqueous mineral carbonation route to reduce the CO2 emissions at the chimney of industrial emitters. The reactivity of serpentinite in a stirred tank reactor was studied for several partial pressures of CO2 (pCO2) (0.4, 0.7, 1.3 and 1.6 bar). Prior to carbonation, the feedstock was finely grinded and dehydroxyled at 650 °C by a thermal treatment...
April 21, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28433909/culturing-of-selenastrum-on-diluted-composting-fluids-conversion-of-waste-to-valuable-algal-biomass-in-presence-of-bacteria
#3
Marika Tossavainen, Anne Nykänen, Kalle Valkonen, Anne Ojala, Silja Kostia, Martin Romantschuk
Growth and fatty acid production of microalga Selenastrum sp. with associated bacteria was studied in lab-scale experiments in three composting leachate liquids. Nutrient reduction in cultures was measured at different initial substrate strengths. A small, pilot-scale photobioreactor (PBR) was used to verify lab-scale results. Similar growth conditions supported growth of both Selenastrum and bacteria. CO2 feed enhanced the production of biomass and lipids in PBR (2.4gL(-1) and 17% DW) compared to lab-scale (0...
April 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28427682/effects-and-mechanism-of-diclofenac-degradation-in-aqueous-solution-by-us-zn-0
#4
Ting Huang, Guangming Zhang, Shan Chong, Yucan Liu, Nan Zhang, Shunyan Fang, Jia Zhu
A system of ultrasound radiation coupled with Zn(0) was applied to degrade diclofenac. The effects of initial pH, dosage of Zn(0) and ultrasound density were investigated. To further explore the mechanism of the microcosmic reaction, the fresh and used Zn(0) powders were characterized by SEM, XRD and XPS. Radical scavengers were used to determine the oxidation performance of strong oxidizing free radicals on diclofenac, including hydroxyl radicals and superoxide radicals. The results showed that the optimum removal of diclofenac reached to over 85% at pH of 2...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426376/evaluation-of-different-laser-supported-surgical-protocols-for-the-treatment-of-oral-leukoplakia-a-long-term-follow-up
#5
Samir Nammour, Toni Zeinoun, Amaury Namour, Alain Vanheusden, Paolo Vescovi
PURPOSE: The aim of this study was to evaluate the long-term success rate of oral leukoplakia treatments by using different laser-supported surgical protocols. PATIENTS AND METHODS: Overall, 2347 diagnosed homogeneous oral leukoplakias were treated with CO2 laser and were included in this study. Different surgical protocols (P) were used: P1 (SV = superficial scanning) was a complete superficial vaporization of the leukoplakia by a scanning mode in two passages respecting an overlapping...
April 20, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28421249/co-effects-of-pyrene-and-nitrate-on-the-activity-and-abundance-of-soil-denitrifiers-under-anaerobic-condition
#6
Zhi-Feng Zhou, Yan-Hong Yao, Ming-Xia Wang, Xiao-Hu Zuo
It has previously been confirmed that polycyclic aromatic hydrocarbons (PAHs) could be degraded by soil microbes coupling with denitrification, but the relationships among soil denitrifiers, PAHs, and nitrate under obligate anaerobic condition are still unclear. Here, co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers were investigated through a 45-day incubation experiment. Two groups of soil treatments with (N30) and without (N0) nitrate (30 mg kg(-1) dry soil) amendment were conducted, and each group contained three treatments with different pyrene concentrations (0, 30, and 60 mg kg(-1) dry soil denoted as P0, P30, and P60, respectively)...
April 18, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28414453/global-proteome-profiling-of-a-marine-copepod-and-the-mitigating-effect-of-ocean-acidification-on-mercury-toxicity-after-multigenerational-exposure
#7
Minghua Wang, Jae-Seong Lee, Yan Li
Previously, we found that ocean acidification (OA) mitigates mercury (Hg) toxicity to marine copepod Tigriopus japonicus under multigenerational exposure (four generations, F0-F3). To determine the response mechanisms of T. japonicus against long-term exposure to OA and Hg pollution, we investigated the proteome of F3 copepods after multigenerational exposure to four conditions: pCO2 400 μatm + control; pCO2 1000 μatm + control; pCO2 400 μatm + 1.0 µg/L Hg; and pCO2 1000 μatm + 1.0 µg/L Hg. Functional enrichment analysis indicated that OA enhanced the copepod's energy production mainly by increasing protein assimilation and proteolysis as a compensatory strategy, which explained its physiological resilience to reduced pH...
April 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28413851/detecting-peatland-drains-with-object-based-image-analysis-and-geoeye-1-imagery
#8
J Connolly, N M Holden
BACKGROUND: Peatlands play an important role in the global carbon cycle. They provide important ecosystem services including carbon sequestration and storage. Drainage disturbs peatland ecosystem services. Mapping drains is difficult and expensive and their spatial extent is, in many cases, unknown. An object based image analysis (OBIA) was performed on a very high resolution satellite image (Geoeye-1) to extract information about drain location and extent on a blanket peatland in Ireland...
December 2017: Carbon Balance and Management
https://www.readbyqxmd.com/read/28413256/dynamics-of-postcombustion-co2-capture-plants-modeling-validation-and-case-study
#9
Adam van de Haar, Carsten Trapp, Kai Wellner, Robert de Kler, Gerhard Schmitz, Piero Colonna
The capture of CO2 from power plant flue gases provides an opportunity to mitigate emissions that are harmful to the global climate. While the process of CO2 capture using an aqueous amine solution is well-known from experience in other technical sectors (e.g., acid gas removal in the gas processing industry), its operation combined with a power plant still needs investigation because in this case, the interaction with power plants that are increasingly operated dynamically poses control challenges. This article presents the dynamic modeling of CO2 capture plants followed by a detailed validation using transient measurements recorded from the pilot plant operated at the Maasvlakte power station in the Netherlands...
February 22, 2017: Industrial & Engineering Chemistry Research
https://www.readbyqxmd.com/read/28412567/leachate-treatment-in-landfills-is-a-significant-n2o-source
#10
Xiaojun Wang, Mingsheng Jia, Chengliang Zhang, Shaohua Chen, Zucong Cai
The importance of methane (CH4) emissions from landfills has been extensively documented, while the nitrous oxide (N2O) emissions from landfills are considered negligible. In this study, three landfills were selected to measure CH4 and N2O emissions using the static chamber method. Dongbu (DB) and Dongfu (DF) landfills, both located in Xiamen city, Fujian Province, were classified as sanitary. The former started to receive solid waste from Xiamen city in 2009, and the latter was closed in 2009. Nanjing (NJ) landfill, located in Nanjing county, Fujian Province, was classified as managed...
April 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28410503/continuous-micro-current-stimulation-to-upgrade-methanolic-wastewater-biodegradation-and-biomethane-recovery-in-an-upflow-anaerobic-sludge-blanket-uasb-reactor
#11
Guangyin Zhen, Xueqin Lu, Takuro Kobayashi, Lianghu Su, Gopalakrishnan Kumar, Péter Bakonyi, Yan He, Periyasamy Sivagurunathan, Nándor Nemestóthy, Kaiqin Xu, Youcai Zhao
The dispersion of granules in upflow anaerobic sludge blanket (UASB) reactor represents a critical technical issue in methanolic wastewater treatment. In this study, the potentials of coupling a microbial electrolysis cell (MEC) into an UASB reactor for improving methanolic wastewater biodegradation, long-term process stability and biomethane recovery were evaluated. The results indicated that coupling a MEC system was capable of improving the overall performance of UASB reactor for methanolic wastewater treatment...
April 5, 2017: Chemosphere
https://www.readbyqxmd.com/read/28410484/microalgae-screening-under-co2-stress-growth-and-micro-nutrients-removal-efficiency
#12
Fida Hussain, Syed Zahir Shah, Wenguang Zhou, Munawar Iqbal
Algae are one of the promising agents for greenhouse gas reduction and biofuel production. Different technologies have been developed and introduced in last decades for algae growth. Algae plays a very imperative role in the aquatic ecosystem regarding CO2 reduction and micro-nutrient removal. In present investigation, eight locally isolated (microalgae) strains and two pure strains were studied. The selected microalgae were grown under variable CO2 concentration and CO2 biofixation efficiencies along with micro-nutrient removal were monitored...
March 30, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28407675/-extracorporeal-co2-elimination-ecco2r-for-hypercapnic-respiratory-failure-from-pathophysiology-to-clinical-application
#13
C Karagiannidis, A Philipp, S Strassmann, S Schäfer, M Merten, W Windisch
Extracorporeal CO2 removal (ECCO2R) is becoming an increasingly established treatment option for patients with acute severe hypercapnic respiratory failure. Technically, pumpless arterio-venous systems using the natural arterio-venous pressure gradient and also pump-driven veno-venous systems are available. Here, veno-venous ECCO2R has become the preferred technique, as settings for arterio-venous ECCO2R are restricted and side effects are more common with arterio-venous ECCO2R. Using veno-venous ECCO2R with blood flow rates up to 450 ml/min 60 to 80 ml CO2 can be removed per minute corresponding to 20 to 30 % of the total amount of CO2 production...
April 2017: Pneumologie
https://www.readbyqxmd.com/read/28406930/eutopic-ectopic-endometrial-apoptosis-initiated-by-bilateral-uterine-artery-occlusion-a-new-therapeutic-mechanism-for-uterus-sparing-surgery-in-adenomyosis
#14
Li Chen, Caixia Li, Jing Guo, Ning Luo, Xiaoyan Qu, Le Kang, Mingmin Liu, Zhongping Cheng
The objective of the present study was to investigate differences in the expression of apoptosis-related factors in the eutopic and ectopic endometrium (EuE/EE) in women with adenomyosis before and after laparoscopic bilateral uterine artery occlusion (LUAO). Ten patients with uterine adenomyosis who received LUAO were selected as the research subjects, from whom EuE and EE tissues were obtained before and after LUAO and detected for the expression of apoptosis-related molecules in EuE and EE by PT-PCR and Western blot, and changes in the mitochondrial structure by electron microscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28406544/halogen-free-synthesis-of-carbamates-from-co2-and-amines-using-titanium-alkoxides
#15
Jun-Chul Choi, Hao-Yu Yuan, Norihisa Fukaya, Syun-Ya Onozawa, Qiao Zhang, Seong Jib Choi, Hiroyuki Yasuda
The direct synthesis of carbamates from amines and carbon dioxide in the presence of Ti(OR)4 (R = nBu (1), Me (2), Et (3), nPr (4)) was investigated. Aniline was reacted with the titanium n-butoxide (1) in the presence of carbon dioxide (5 MPa) to give the corresponding n-butyl N-phenylcarbamate (BPC) in nearly quantitative yield (99%) within 20 min. Furthermore, 1 could be regenerated upon reaction with n-butanol during water removal. The recovered 1 could then be reused in a subsequent reaction.
April 12, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28406154/pathways-for-balancing-co2-emissions-and-sinks
#16
Brian Walsh, Philippe Ciais, Ivan A Janssens, Josep Peñuelas, Keywan Riahi, Felicjan Rydzak, Detlef P van Vuuren, Michael Obersteiner
In December 2015 in Paris, leaders committed to achieve global, net decarbonization of human activities before 2100. This achievement would halt and even reverse anthropogenic climate change through the net removal of carbon from the atmosphere. However, the Paris documents contain few specific prescriptions for emissions mitigation, leaving various countries to pursue their own agendas. In this analysis, we project energy and land-use emissions mitigation pathways through 2100, subject to best-available parameterization of carbon-climate feedbacks and interdependencies...
April 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28405055/stability-of-a-benzyl-amine-based-co2-capture-adsorbent-in-view-of-regeneration-strategies
#17
Qian Yu, Jorge de la P Delgado, Rens Veneman, Derk W F Brilman
In this work, the chemical and thermal stability of a primary amine-functionalized ion-exchange resin (Lewatit VP OC 1065) is studied in view of the potential options of regenerating this sorbent in a CO2 removal application. The adsorbent was treated continuously in the presence of air, different O2/CO2/N2 mixtures, concentrated CO2, and steam, and then the remaining CO2 adsorption capacity was measured. Elemental analysis, BET/BJH analysis, Fourier transform infrared spectroscopy, and thermogravimetric analysis were applied to characterize adsorbent properties...
March 29, 2017: Industrial & Engineering Chemistry Research
https://www.readbyqxmd.com/read/28402311/polishing-of-municipal-secondary-effluent-using-native-microalgae-consortia
#18
Julio César Beltrán-Rocha, Icela Dagmar Barceló-Quintal, Magdalena García-Martínez, Luis Osornio-Berthet, Nidia Saavedra-Villarreal, Juan Villarreal-Chiu, Ulrico Javier López-Chuken
This work evaluates the use of native microalgae consortia for a dual role: polishing treatment of municipal wastewater effluents and microalgae biomass feedstock potential for biodiesel or biofertilizer production. An initial screening was undertaken to test N and P removal from secondary effluents and biomass production by 12 consortia. A subsequent treatment was performed by selected consortia (01 and 12) under three operational conditions: stirring (S), S + 12 h of daily aeration (S + A) and S + A enriched with CO2 (S + AC)...
April 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28402093/extracorporeal-co2-removal-ecco2r-in-critically-ill-patients-a-systematic-review
#19
Fabio S Taccone, Maximilian V Malfertheiner, Fiorenza Ferrari, Matteo DI Nardo, Justyna Swol, Lars M Broman, Leen Vercaemst, Nicholas Barrett, Federico Pappalardo, Jan Belohlavek, Thomas Mueller, Roberto Lorusso, Mirko Belliato
INTRODUCTION: The use of extracorporeal CO2 removal (ECCO2R) is increasingly employed in critically ill patients. However, the clinical evidence supporting its efficacy remains currently poor. EVIDENCE ACQUISITION: A systematic review using MEDLINE via PubMed was performed to identify eligible studies (until 30th September 2016). The amount of CO2 reduction, the effect on the duration of mechanical ventilation and weaning, the impact on patients' outcome and the occurrence of complications were evaluated...
April 11, 2017: Minerva Anestesiologica
https://www.readbyqxmd.com/read/28399695/the-effect-of-adenotonsillectomy-on-obstructive-sleep-apnea-in-children-with-down-syndrome
#20
Mosaad Abdel-Aziz, Khaled Azooz, Nader Naguib, Ramez Reda, Ahmed Kamel
OBJECTIVE: Children with Down syndrome (DS) are liable to develop obstructive sleep apnea (OSA) due to many anatomical airway abnormalities. The tonsils and adenoid occupy part of the airway space, and their removal may be helpful in relieving airway obstruction. The aim of this study was to assess the effectiveness of adenotonsillectomy in the treatment of OSA in those children. METHODS: Fifty DS children with difficult breathing were recruited, and they were subjected to polysomnographic examination (PSG)...
April 11, 2017: Acta Oto-laryngologica
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