keyword
MENU ▼
Read by QxMD icon Read
search

Extracellular matrix

keyword
https://www.readbyqxmd.com/read/29169185/co-culture-of-human-alveolar-epithelial-haelvi-and-macrophage-thp-1-cell-lines
#1
Stephanie Kletting, Sarah Barthold, Urska Repnik, Gareth Griffiths, Brigitta Loretz, Nicole Schneider-Daum, Cristiane de Souza Carvalho-Wodarz, Claus-Michael Lehr
The air-blood barrier is mainly composed of alveolar epithelial cells and macrophages. Whereas the epithelium acts as a diffusional barrier, macrophages represent an immunological barrier, in particular for larger molecules and nanoparticles. This paper describes a new co-culture of human cell lines representing both cell types. Acquiring, culturing and maintaining primary alveolar epithelial cells presents significant logistical and technical difficulties. The recently established human alveolar epithelial lentivirus immortalized cell line, hAELVi, when grown on permeable filters, form monolayers with high functional and morphological resemblance to alveolar type I cells...
November 23, 2017: ALTEX
https://www.readbyqxmd.com/read/29168879/1p36-deletion-results-in-a-decrease-in-glycosaminoglycans-which-is-associated-with-aggressiveness-in-neuroblastic-tumors
#2
Irene Tadeo, Esther Gamero-Sandemetrio, Ana P Berbegall, Samuel Navarro, Adela Cañete, Rosa Noguera
Despite our deep understanding of neuroblastic tumors, some patients still suffer treatment failure, so pre-treatment risk stratification still requires improvement and the search for new therapeutic targets must continue. Here we correlated prognostic clinical and biological features of neuroblastic tumors with the density of extracellular matrix glycosaminoglycans (the main components of the extracellular matrix 'ground substance'), in nearly 400 primary samples. We also studied the relationship between the density of extracellular matrix glycosaminoglycans and the expression of B3GALT6, an enzyme required for their synthesis...
November 23, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/29168704/extracellular-polymeric-substance-production-in-high-rate-algal-oxidation-ponds
#3
Taobat A Jimoh, A Keith Cowan
Integrated algal pond systems (IAPSs) combine anaerobic and aerobic bioprocesses to affect sewage treatment. The present work describes the isolation and partial characterisation of soluble extracellular polymeric substances (EPSs) associated with microalgal bacterial flocs (MaB-flocs) generated in high rate algal oxidation ponds (HRAOPs) of an IAPS treating domestic sewage. Productivity and change in MaB-flocs concentration, measured as mixed liquor suspended solids (MLSS) between morning (MLSSAM) and evening (MLSSPM) were monitored and the substructure of the MaB-flocs matrix examined by biochemical analysis and Fourier transform infrared spectroscopy (FT-IR)...
November 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29168660/the-interplay-between-extracellular-matrix-remodelling-and-kinase-signalling-in-cancer-progression-and-metastasis
#4
Joanna N Skhinas, Thomas R Cox
The extracellular matrix (ECM) is a master regulator of cellular phenotype and behaviour. It plays a crucial role in both normal tissue homeostasis and complex diseases such as cancer. The interplay between the intrinsic factors of cancer cells themselves, including their genotype and signalling networks; and the extrinsic factors of the tumour stroma, such as the ECM and ECM remodelling; together determine the fate and behaviour of cancer cells. As a consequence, tumour progression, metastatic spread and response to therapy are ultimately controlled by ECM-driven fine-tuning of intracellular kinase signalling...
November 23, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/29167937/novel-comprehensive-multidimensional-liquid-chromatography-approach-for-elucidation-of-the-microbosphere-of-shikimate-producing-escherichia-coli-sp1-1-pkd15-071-strain
#5
Francesco Cacciola, Domenica Mangraviti, Francesca Rigano, Paola Donato, Paola Dugo, Luigi Mondello, Hernan J Cortes
Shikimic acid is a intermediate of aromatic amino acid biosynthesis and the preferred starting material for production of the most commonly prescribed anti-influenza drug, Tamiflu. Its six-membered carbocyclic ring is adorned with several chiral centers and various functionalities, making shikimic acid a valuable chiral synthon. When microbially-produced, in addition to shikimic acid, numerous other metabolites are exported out of the cytoplasm and accumulate in the culture medium. This extracellular matrix of metabolites is referred to as the microbosphere...
November 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29167844/recellularization-of-decellularized-adipose-tissue-derived-stem-cells-role-of-the-cell-secreted-extracellular-matrix-in-cellular-differentiation
#6
V Guneta, Z Zhou, N S Tan, S Sugii, M T C Wong, C Choong
Adipose-derived stem cells (ASCs) are found in a location within the adipose tissue known as the stem cell niche. The ASCs in the niche are maintained in the quiescent state, and upon exposure to various microenvironmental triggers are prompted to undergo proliferation or differentiation. These microenvironmental triggers also modulate the extracellular matrix (ECM), which interacts with the cells through the cytoskeleton and induces downstream events inside the cells that bring about a change in cell behaviour...
November 23, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/29167826/efficient-in-vitro-delivery-of-noggin-sirna-enhances-osteoblastogenesis
#7
S Ghadakzadeh, R C Hamdy, M Tabrizian
Several types of serious bone defects would not heal without invasive clinical intervention. One approach to such defects is to enhance the capacity of bone-formation cells. Exogenous bone morphogenetic proteins (BMP) have been utilized to positively regulate matrix mineralization and osteoblastogenesis, however, numerous adverse effects are associated with this approach. Noggin, a potent antagonist of BMPs, is an ideal candidate to target and decrease the need for supraphysiological doses of BMPs. In the current research we report a novel siRNA-mediated gene knock-down strategy to down-regulate Noggin...
November 2017: Heliyon
https://www.readbyqxmd.com/read/29167509/cartilage-intermediate-layer-protein-1-cilp1-a-novel-mediator-of-cardiac-extracellular-matrix-remodelling
#8
Frans A van Nieuwenhoven, Chantal Munts, Roel C Op't Veld, Arantxa González, Javier Díez, Stephane Heymans, Blanche Schroen, Marc van Bilsen
Heart failure is accompanied by extracellular matrix (ECM) remodelling, often leading to cardiac fibrosis. In the present study we explored the significance of cartilage intermediate layer protein 1 (CILP1) as a novel mediator of cardiac ECM remodelling. Whole genome transcriptional analysis of human cardiac tissue samples revealed a strong association of CILP1 with many structural (e.g. COL1A2 r(2) = 0.83) and non-structural (e.g. TGFB3 r(2) = 0.75) ECM proteins. Gene enrichment analysis further underscored the involvement of CILP1 in human cardiac ECM remodelling and TGFβ signalling...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167507/characterization-of-the-early-molecular-changes-in-the-glomeruli-of-cd151-mice-highlights-induction-of-mindin-and-mmp-10
#9
Crystal Naudin, Brian Smith, Danielle R Bond, Matthew D Dun, Rodney J Scott, Leonie K Ashman, Judith Weidenhofer, Séverine Roselli
In humans and FVB/N mice, loss of functional tetraspanin CD151 is associated with glomerular disease characterised by early onset proteinuria and ultrastructural thickening and splitting of the glomerular basement membrane (GBM). To gain insight into the molecular mechanisms associated with disease development, we characterised the glomerular gene expression profile at an early stage of disease progression in FVB/N Cd151 (-/-) mice compared to Cd151 (+/+) controls. This study identified 72 up-regulated and 183 down-regulated genes in FVB/N Cd151 (-/-) compared to Cd151 (+/+) glomeruli (p < 0...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167167/cellular-mechanisms-underlying-obesity-induced-arterial-stiffness
#10
Annayya R Aroor, Guanghong Jia, James R Sowers
Obesity is an emerging pandemic driven by consumption of a diet rich in high fat and high refined carbohydrates (western diet [WD]) and a sedentary life style in both children and adults. There is mounting evidence that arterial stiffness in obesity is an independent and strong predictor of adverse cardiovascular disease (CVD), cognitive functional decline and chronic kidney disease. Cardiovascular stiffness is a precursor to systolic hypertension, cardiac diastolic dysfunction, impairment of coronary and cerebral blood flow, and atherosclerosis...
November 22, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29167120/acute-lysyl-oxidase-inhibition-alters-microvascular-function-in-normotensive-but-not-hypertensive-men-and-women
#11
Daniel H Craighead, Huilei Wang, Lakshmi Santhanam, Lacy M Alexander
The Lysyl oxidase (LOX) family of enzymes regulates collagen cross-linking. LOX is upregulated in hypertension, increasing vascular stiffness. In vivo human research is sparse as long-term LOX inhibition in animals causes vascular instability. Our aim was to evaluate the effects of LOX inhibition on cutaneous microvascular function to determine if LOX function was upregulated in hypertensive humans. Four intradermal microdialysis fibers were placed in the forearm of 10 young (age: 24±1 y, MAP: 87±2 mmHg), 10 normotensive (age: 50±2 y, MAP: 84±1 mmHg), and 10 hypertensive (age: 53±2 y, MAP 112±2 mmHg) subjects...
November 22, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29166920/a-3d-epithelial-mesenchymal-co-culture-model-of-human-bronchial-tissue-recapitulates-multiple-features-of-airway-tissue-remodeling-by-tgf-%C3%AE-1-treatment
#12
Shinkichi Ishikawa, Kanae Ishimori, Shigeaki Ito
BACKGROUND: The collagen gel contraction assay measures gel size to assess the contraction of cells embedded in collagen gel matrices. Using the assay with lung fibroblasts is useful in studying the lung tissue remodeling process in wound healing and disease development. However, the involvement of bronchial epithelial cells in this process should also be investigated. METHODS: We applied a layer of mucociliary differentiated bronchial epithelial cells onto collagen gel matrices with lung fibroblasts...
November 22, 2017: Respiratory Research
https://www.readbyqxmd.com/read/29166713/-advances-in-the-research-of-molecular-mechanism-of-negative-pressure-wound-therapy-in-improving-wound-healing
#13
Y Liu, D H Hu
Recently, negative pressure wound therapy (NPWT) is a rising technology to improve wound healing. In clinical application, it benefits fast debridement and wound close, limits infection, and promotes wound healing. It is an effective therapy for all kinds of acute or chronic wound. Currently, researches demonstrate that NPWT promotes angiogenesis, granulation tissue growth, and extracellular matrix remodeling through regulating the signaling of anti-inflammatory cytokines, mechanicalreceptor and chemoreceptor, which is related to several growth factors and inflammatory factors...
November 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29166254/proactive-and-early-aggressive-wound-management-a-shift-in-strategy-developed-by-a-consensus-panel-examining-the-current-science-prevention-and-management-of-acute-and-chronic-wounds
#14
Gregory A Bohn, Gregory S Schultz, Brock A Liden, Michael N Desvigne, Eric J Lullove, Igor Zilberman, Mary B Regan, Marta Ostler, Karen Edwards, Georgia M Arvanitis, Jodi F Hartman
Normal wound healing is accomplished through a series of well-coordinated, progressive events with overlapping phases. Chronic wounds are described as not progressing to healing or not being responsive to management in a timely manner. A consensus panel of multidisciplinary wound care professionals was assembled to (1) educate wound care practitioners by identifying key principles of the basic science of chronic wound pathophysiology, highlighting the impact of metalloproteinases and biofilms, as well as the role of the extracellular matrix; and (2) equip practitioners with a systematic strategy for the prevention and healing of acute injuries and chronic wounds based upon scientific evidence and the panel members' expertise...
November 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29166241/two-classes-of-secreted-synaptic-organizers-in-the-central-nervous-system
#15
Michisuke Yuzaki
Research in the last two decades has identified many synaptic organizers in the central nervous system that directly regulate the assembly of preand/ or postsynaptic molecules, such as synaptic vesicles, active zone proteins, and neurotransmitter receptors. They are classified into secreted factors and cell adhesion molecules, such as neurexins and neuroligins. Certain secreted factors are termed extracellular scaffolding proteins (ESPs) because they are components of the synaptic extracellular matrix and serve as a scaffold at the synaptic cleft...
November 20, 2017: Annual Review of Physiology
https://www.readbyqxmd.com/read/29165552/what-happened-to-the-igf-binding-proteins
#16
Leon A Bach
Insulin-like growth factor binding proteins (IGFBPs) 1-6 are high affinity regulators of IGF activity. They generally inhibit IGF actions by preventing binding to the IGF-I receptor but may also enhance their actions under some conditions. Post-translational modifications such as glycosylation and phosphorylation modulate IGFBP properties, and IGFBP proteolysis results in IGF release. IGFBPs have more recently been shown to have IGF-independent actions. A number of mechanisms are involved, including modulation of other growth factor pathways, nuclear localization and transcriptional regulation, interaction with the sphingolipid pathway, and binding to non-IGF biomolecules in the extracellular space and matrix, on the cell surface and intracellularly...
November 20, 2017: Endocrinology
https://www.readbyqxmd.com/read/29164819/decellularized-bovine-cotyledons-may-serve-as-biological-scaffolds-with-preserved-vascular-arrangement
#17
Rodrigo da Silva Nunes Barreto, Patricia Romagnolli, Andrea Maria Mess, Maria Angelica Miglino
Technically produced scaffolds are common to establish transplantable tissues for Regenerative Medicine, but also biological ones that are closer to the natural condition become of interest. Placentas are promising, because they represented available, complete organs with rich extracellular matrix (ECM) and well developed vasculature that easily could build anastomoses to a host´s organ. Only placentas from larger animal models such as the bovine meet the dimensions large enough for most organs, but are not adequately described yet...
November 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29164771/functional-self-assembled-peptide-scaffold-inhibits-tnf-alpha-induced-inflammation-and-apoptosis-in-nucleus-pulposus-cells-by-suppressing-nf-%C3%AE%C2%BAb-signaling
#18
Xiaochuan Li, Shi Cheng, Yaohong Wu, Jingwei Ying, Chaofeng Wang, Tianyong Wen, Xuedong Bai, Wei Ji, Deli Wang, Dike Ruan
Although nucleus pulposus (NP) tissue engineering has achieved tremendous success, researches still face the huge obstacles in maintaining cell survival and function. A novel functional self-assembled peptide RADA-KPSS was constructed by conjugating BMP-7 short active fragment (KPSS) to the C-terminus of RADA16-I that displays anti-inflammatory and anti-apoptosis effects. However, whether this functional self-assembled RADA-KPSS peptide can alleviate inflammation and NPC apoptosis induced by TNF-α has not been studied...
November 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29164232/mast-cell-derived-tryptase-in-geographic-atrophy
#19
D Scott McLeod, Imran Bhutto, Malia M Edwards, Manasee Gedam, Rajkumar Baldeosingh, Gerard A Lutty
Purpose: Our previous study demonstrated significantly more degranulating mast cells (MCs) in choroids from subjects with age-related macular degeneration compared to aged controls. This study examined the immunolocalization of tryptase, the most abundant MC secretory granule-derived serine protease, in aged control eyes and eyes with geographic atrophy (GA). Methods: Postmortem human eyes with and without GA were obtained from the National Disease Research Interchange...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29164186/phenylalanine-and-derivatives-as-versatile-low-molecular-weight-gelators-design-structure-and-tailored-function
#20
REVIEW
Tanmay Das, Marleen Häring, Debasish Haldar, David Díaz Díaz
Phenylalanine (Phe) is an essential amino acid classified as neutral and nonpolar due to the hydrophobic nature of the benzyl side chain. In the field of materials science, the chemical modification of phenylalanine at C or N terminus has enabled to synthesize a large number of low-molecular-weight gelators over the past decade. Thus, many physical (or supramolecular) softgel materials have been fabricated by self-assembly of Phe-derived building blocks, which can be programmed with atomic level information and modification...
November 22, 2017: Biomaterials Science
keyword
keyword
9723
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"