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Juan Aznar-Poveda, Jose Antonio Lopez-Pastor, Antonio-Javier Garcia-Sanchez, Joan Garcia-Haro, Toribio Fernández Otero
Traditionally, electrochemical procedures aimed at determining substance concentrations have required a costly and cumbersome laboratory environment. Specialized equipment and personnel obtain precise results under complex and time-consuming settings. Innovative electrochemical-based sensors are emerging to alleviate this difficulty. However, they are generally scarce, proprietary hardware and/or software, and focused only on measuring a restricted range of substances. In this paper, we propose a portable, flexible, low-cost system, built from commercial off-the-shelf components and easily controlled, using open-source software...
February 10, 2018: Sensors
Soon Sung Kwon, Byungkwang Kim, Sung Won Hwang, Kwangwoo Kim, Duck Jin Hong, Seok Hoon Jeong, Jeong-Ho Kim
No abstract text is available yet for this article.
November 1, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Jehoon Lee, Myungshin Kim, Hyojin Chae, Yeongsic Kim, Hae-il Park, Yonggoo Kim, Hyunyoung Chi, Hi Jeong Kwon
BACKGROUND: A novel enzymatic HbA1c assay was introduced for use in an automated chemistry analyzer. With this unique method, HbA1c and plasma glucose can be measured from the same EDTA tube. We evaluated the analytical performance of this enzymatic HbA1c assay in a JCA-BM6010/C analyzer and compared the HbA1c values with the results from other widely used methodological instruments. METHODS: The imprecision, linearity, carry-over and concordance rate of the enzymatic HbA1c test (BM Test HbA1c) using the JCA-BM6010/C analyzer were evaluated...
November 2013: Clinical Chemistry and Laboratory Medicine: CCLM
(no author information available yet)
No abstract text is available yet for this article.
March 1, 1970: Analytical Chemistry
Olga Krystofova, Libuse Trnkova, Vojtech Adam, Josef Zehnalek, Jaromir Hubalek, Petr Babula, Rene Kizek
Routine determination of trace metals in complex media is still a difficult task for many analytical instruments. The aim of this work was to compare three electro-chemical instruments [a standard potentiostat (Autolab), a commercially available miniaturized potentiostat (PalmSens) and a homemade micropotentiostat] for easy-to-use and sensitive determination of cadmium(II) and lead(II) ions. The lowest detection limits (hundreds of pM) for both metals was achieved by using of the standard potentiostat, followed by the miniaturized potentiostat (tens of nM) and the homemade instrument (hundreds of nM)...
2010: Sensors
Lázaro Alessandro Soares Nunes, René Brenzikofer, Denise Vaz Macedo
OBJECTIVES: Our aims were to test a liquid-based saliva collection system for total antioxidant status (TAS), uric acid (UA), total protein concentration (TP) and salivary alpha-amylase (SAA) activity; to determine if these analytes in serum and saliva are correlated in a physically active population and to establish reference intervals for these parameters. DESIGN AND METHODS: Participants in this study were 115 physically active males (18-20 years old). Saliva samples were collected using the Saliva Collection System (Greiner Bio-One) immediately before obtaining blood...
December 2011: Clinical Biochemistry
Lázaro Alessandro S Nunes, René Brenzikofer, Denise Vaz de Macedo
The analysis of blood constituents allows the detection of various physiological or pathological states when their values are increased or decreased in relation to a well-defined reference group or to themselves if monitored longitudinally. In the latter case, it is important to know the reference change value (RCV) or critical difference, which defines the percentage change that should be exceeded-given the analytical and biological variations inherent to a particular test, in that there is a significant difference between the two consecutive measurements...
September 2010: European Journal of Applied Physiology
Kemal Ahmed, Gonfa Ayana, Ephrem Engidawork
BACKGROUND: Lead exposure is common in automobile battery manufacture and repair, radiator repair, secondary smelters and welding units. Urinary Aminolevulinic acid has validity as a surrogate measure of blood lead level among workers occupationally exposed to lead. This study had therefore assessed the magnitude of lead exposure in battery repair workers of three transport service enterprises. METHODS: To this effect, a cross-sectional study was carried out on lead exposure among storage battery repair workers between November 2004 and May 2005 from Anbasa, Comet and Walia transport service enterprises, Addis Ababa, Ethiopia...
November 28, 2008: Journal of Occupational Medicine and Toxicology
Rosa Fireman Dutra, Renata Kelly Mendes, Valdinete Lins da Silva, Lauro Tatsuo Kubota
The cardiac troponin T (cTnT) is specific biomarker important for trials of acute myocardial infarctions (AMI). In this paper, a SPR sensor in real time to detect the biomarker was developed on a commercially available surface plasmon resonance AUTOLAB SPIRIT. The cTnT receptor molecule was covalently immobilized on a gold substrate via a self-assembled monolayer (SAM) of thiols by using cysteamine-coupling chemistry. This biosensor presented a linear response range for cTnT between 0.05 and 4.5 ng/mL (r=0...
April 11, 2007: Journal of Pharmaceutical and Biomedical Analysis
Charles D Hawker, Susan B Garr, Leslie T Hamilton, John R Penrose, Edward R Ashwood, Ronald L Weiss
BACKGROUND: Our laboratory, a large, commercial, esoteric reference laboratory, sought some form of total laboratory automation to keep pace with rapid growth of specimen volumes as well as to meet competitive demands for cost reduction and improved turnaround time. METHODS: We conducted a systematic evaluation of our needs, which led to the development of a plan to implement an automated transport and sorting system. We systematically analyzed and studied our specimen containers, test submission requirements and temperatures, and the workflow and movement of people, specimens, and information throughout the laboratory...
October 2002: Clinical Chemistry
S R Middleton
BACKGROUND: Trends in laboratory automation and critical project principles and design concepts are presented. APPROACH: MDS AutoLab technology development and automation projects were reviewed. Successful methods and approaches were extracted. ISSUES: Continued pressure on the laboratory to reduce costs and increase productivity has catalyzed dramatic development in laboratory automation. Today, laboratories can choose from a wide range of options...
May 2000: Clinical Chemistry
J H Ngui-Yen, P W Doyle, J A Smith
Two rapid automated methods, the Syva EMIT Autolab 6000 (Syva Corp., Syntex Diagnostics, Ottawa, Ontario, Canada) and the Abbott TDX (Abbott Diagnostics, Mississauga, Ontario, Canada) systems, for determining amikacin, gentamicin and tobramycin levels in serum were evaluated for accuracy, reproducibility, and cost. Both methods had a high degree of accuracy and reproducibility in assaying gentamicin, amikacin, and tobramycin. The Abbott TDX required an average of only 1 min and 43 s for a single assay compared with 4 min and 15 s for the Syva EMIT automated system...
November 1984: Journal of Clinical Microbiology
R H Glew, R A Pavuk
Two fully automated drug-monitoring systems, the Syva Autolab 6000 and the Beckman Auto ICS, were compared in terms of accuracy, precision, speed of operation, and cost effectiveness in the determination of gentamicin levels in serum. Within-run and between-run precision for both systems were acceptable (coefficient of variation, 2.0 to 6.9%), and patient sample comparisons resulted in an intermethod correlation coefficient of 0.96. When reference samples (prepared to contain 1.2 to 10 micrograms of gentamicin per ml) were assayed, the Syva Autolab 6000 obtained concentrations within 8% of expected values, whereas the Beckman Auto ICS reported values up to 17% higher than target values...
January 1985: Journal of Clinical Microbiology
R Ernst, L Williams, M Dalbey, C Collins, S Pankey
EMIT tests are available for quantitative determination of the tricyclic antidepressants amitriptyline (AMI), nortriptyline (NORT), imipramine (IMI), and desipramine (DMI). An extraction step before analysis eliminates cross-reacting polar metabolites. Excellent correlation between EMIT and high-performance liquid chromatography (HPLC) has been previously established. Most published protocols for using EMIT reagents on COBAS-BIO centrifugal analyzers were designed for analytes present at 1-50 mg/L in serum...
1987: Therapeutic Drug Monitoring
C R Lehmann, K J Boran, W P Pierson, A P Melikian, G J Wright
Plasma quinidine results determined by enzyme multiplied immunoassay (EMIT) (Syva) were compared with results from a quinidine-specific high performance liquid chromatographic (HPLC) assay. During a clinical study, 16 patients with stable ventricular arrhythmias were treated with three oral quinidine preparations given during three consecutive 3-day periods. Seventeen plasma samples were drawn from each patient at steady-state during each period. Each specimen was divided into two portions, one for assay by EMIT and the other for assay by HPLC...
1986: Therapeutic Drug Monitoring
P Wells, E Robbins, R Cowley, P Noone
The Syva EMIT Autolab 6000 and the Abbott TDX automated methods for measuring serum aminoglycoside concentrations were compared with each other and with a new semiautomated version of the urease technique. Each was evaluated for accuracy, reproducibility, cost, and ease of use. All three methods gave satisfactory results, although the Abbott TDX was the most accurate. Both automated methods gave results within minutes, whereas the new urease method, which involved more initial manipulation, took 1.25 h to complete (mainly incubation time)...
September 1987: Journal of Clinical Microbiology
J Ziegenhorn
A simple kinetic method for the automated determination of total serum cholesterol was developed using cholesterol esterase, cholesterol oxidase, catalase and the color reaction according to Hantzsch. The procedure has been adapted to the Braun SysteMatik, Greiner GSA II, Eppendorf 5032, Lars Ljungberg Autolab, Perkin-Elmer C4 and Perkin-Elmer C4B analyzers. The variants of the method yielded satisfactory results with regard of precision, accuracy and sensitivity to interferences.
March 1975: Zeitschrift Für Klinische Chemie und Klinische Biochemie
K Besserer, H G König, A Moosmayer
No abstract text is available yet for this article.
1977: Beiträge Zur Gerichtlichen Medizin
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