keyword
MENU ▼
Read by QxMD icon Read
search

ion chromatography

keyword
https://www.readbyqxmd.com/read/28719139/identification-of-metabolites-in-human-and-rat-urine-after-oral-administration-of-xiao-qing-long-tang-granule-using-ultra-high-performance-liquid-chromatography-combined-with-quadrupole-time-of-flight-mass-spectrometry
#1
Lei Zhou, Qiang Zhang, Wen Qi, Shuai Yan, Jialin Qu, Toshiaki Makino, Dan Yuan
Xiao-Qing-Long-Tang is a traditional Chinese formula used for the treatment of cold syndrome, bronchitis, and nasal allergies for thousands of years. However, the in vivo integrated metabolism of its multiple components and the active chemical constituents of Xiao-Qing-Long-Tang remain unknown. In this study, a method using ultra high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was established for the detection and identification of the metabolites in human and rat urine after oral administration of Xiao-Qing-Long-Tang...
July 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28719082/determination-of-seven-drugs-of-abuse-and-their-metabolites-in-surface-and-waste-water-using-solid-phase-extraction-coupled-to-liquid-chromatography-with-high-resolution-mass-spectrometry
#2
Helena Prosen, Núria Fontanals, Francesc Borrull, Rosa Maria Marcé
A method based on liquid chromatography with electrospray ionization high-resolution mass spectrometry (Exactive Orbitrap) combined with solid-phase extraction using a strong cationic exchange mixed-mode sorbent has been developed for the determination of seven drugs of abuse, including two synthetic cathinones, as well as some of their metabolites in environmental water samples. The method provides low detection limits and a high confirmation power thanks to the diagnostic and two fragment ions monitored for each compound in high-resolution mass spectrometry, providing six identification points for each analyte...
July 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28718961/photodegradation-kinetics-transformation-and-toxicity-prediction-of-ketoprofen-carprofen-and-diclofenac-acid-in-aqueous-solutions
#3
Jian Li, Li-Yun Ma, Lu-Shuang Li, Li Xu
In this study, the photodegradation of three commonly used non-steroidal anti-inflammatory drugs, ketoprofen (KET), carprofen (CAR) and diclofenac acid (DIC), was conducted under UV irradiation. The kinetic results showed that the studied three pharmaceuticals obeyed to the first-order reaction with the decreasing rate constants of 1.54×10(-4) s(-1) , 5.91×10(-5) s(-1) and 7.78×10(-6) s(-1) for CAR, KET and DIC, respectively. Moreover, the main transformation products (TPs) were identified by ion-pair liquid-liquid extraction combined with injection port derivatization-gas chromatography-mass spectrometer and high performance liquidchromatography-quadrupole-time of flight mass spectrometer analysis...
July 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28718960/chemical-uplc-esi-ms-ms-profiling-of-aconitum-alkaloids-and-their-metabolites-in-rat-plasma-and-urine-after-oral-administration-of-aconitum-carmichaelii-debx-root-extract
#4
Mingjie Zhang, Manman Wang, Jiajia Liang, Yongqing Wen, Zhili Xiong
In this paper, an ultra high performance liquid chromatography tandem mass spectrometric (UPLC-ESI-MS/MS) method in positive ion mode was established to systematically identify and to compare the major aconitum alkaloids and their metabolites in rat plasma and urine after oral administration of Fuzi extract. A total twenty-nine components including twenty-five C19 -diterpenoid alkaloids and four C20 -diterpenoid alkaloids were identified in Fuzi extract. Thirteen of the parent components and five metabolites were detected in rat plasma and sixteen parent compounds and six metabolites in urine...
July 18, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28717896/optimization-of-lipid-extraction-and-analytical-protocols-for-uhplc-esi-hrms-based-lipidomic-analysis-of-adherent-mammalian-cancer-cells
#5
Huizhen Zhang, Yue Gao, Jiahong Sun, Shicheng Fan, Xinpeng Yao, Xiaorong Ran, Chuanqi Zheng, Min Huang, Huichang Bi
Lipidomics, which reveals comprehensive characterization of molecular lipids, is a rapidly growing technology used in biomedical research. Lipid extraction is a critical step in lipidomic analysis. However, the effectiveness of different lipid extract solvent systems from cellular samples still remains unclear. In the current study, the protocol of reverse-phase liquid chromatography mass spectrometry (LC/MS)-based lipidomics was optimized for extraction and detection of lipids from human pancreatic cancer cell line PANC-1...
July 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28715789/quantification-of-idp-73152-a-novel-antibiotic-in-plasma-from-mice-rats-and-humans-using-an-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-method-for-use-in-pharmacokinetic-studies
#6
Myongjae Lee, Dohee Kim, Jeongcheol Shin, Hee-Yeol Lee, Soobong Park, Hong-Sub Lee, Jae-Hoon Kang, Suk-Jae Chung
IDP-73152, a novel inhibitor of a bacterial peptide deformylase, was recently approved as a new, investigational drug in Korea for the clinical management of infections caused by Gram positive bacteria. The objective of this study was to develop/validate a simple and robust analytical method for the determination of IDP-73152 in plasma samples from rodents and humans, and to assess the feasibility of the assay for use in pharmacokinetic studies using animal models. Plasma samples were processed using a standard method for protein precipitation and an aliquot of the extract then injected onto an UHPLC-MS/MS system...
July 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28712550/towards-an-automatic-lab-on-valve-ion-mobility-spectrometric-system-for-detection-of-cocaine-abuse
#7
David J Cocovi-Solberg, Francesc A Esteve-Turrillas, Sergio Armenta, Miguel de la Guardia, Manuel Miró
A lab-on-valve miniaturized system integrating on-line disposable micro-solid phase extraction has been interfaced with ion mobility spectrometry for the accurate and sensitive determination of cocaine and ecgonine methyl ester in oral fluids. The method is based on the automatic loading of 500μL of oral fluid along with the retention of target analytes and matrix clean-up by mixed-mode cationic/reversed-phase solid phase beads, followed by elution with 100μL of 2-propanol containing (3% v/v) ammonia, which are online injected into the IMS...
June 30, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28712549/determination-of-trace-amounts-of-g-type-nerve-agents-in-aqueous-samples-utilizing-in-vial-instantaneous-derivatization-and-liquid-chromatography-tandem-mass-spectrometry
#8
Avi Weissberg, Moran Madmon, Maor Elgarisi, Shai Dagan
A methodology for sensitive determination of sarin (GB), soman (GD) and cyclosarin (GF) chemical warfare agents in aqueous media was developed. The method incorporates direct derivatization with 2-[(dimethylamino)methyl]phenol (2-DMAMP), a commercially available, water-soluble reagent, followed by LC-ESI-MS/MS analysis in the positive ion mode. Five derivatization agents were characterized for their MS/MS fragmentation pattern, and their reaction time, temperature and derivatization-reagent amount were optimized...
July 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28712302/overcoming-the-hydrolytic-lability-of-a-reaction-intermediate-in-production-of-protein-drug-conjugates-conjugation-of-an-acyclic-nucleoside-phosphonate-to-a-model-carrier-protein
#9
Shengsheng Xu, Igor A Kaltashov
Acquired immunodeficiency syndrome (AIDS) remains one of the most serious public health challenges and a significant cause of mortality for certain populations. Despite the large number of antiretrovirals developed in the past two decades, the inability of small molecule therapeutics to target HIV reservoirs directly creates a significant obstacle to their effective utilization. Indeed, achieving the desired therapeutic outcome in the absence of an effective means of targeted delivery must rely on dosage escalation, which frequently causes severe toxicity...
July 17, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28711958/systematic-assessment-of-urinary-hydroxy-oxo-glutarate-for-diagnosis-and-follow-up-of-primary-hyperoxaluria-type-iii
#10
Ada Ventzke, Markus Feldkötter, Andrew Wei, Jutta Becker, Bodo B Beck, Bernd Hoppe
BACKGROUND: There are currently three distinct autosomal recessive inherited types of primary hyperoxaluria (PH: PHI, PHII, and PHIII), all characterized by the endogenous overproduction of oxalate. The PH type is difficult to differentiate by clinical features alone. In addition to universal general characteristics to all hyperoxaluria subtypes, specific urinary metabolites can be detected: glycolate in PHI, L-glyceric acid in PHII, and hydroxy-oxo-glutarate (HOG) in PHIII. PHIII is considered to be the most benign form and is characterized by severe recurrent urolithiasis in early life, followed by clinical remission in many, but not all patients...
July 15, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28711785/a-fit-for-purpose-lc-ms-ms-method-for-the-simultaneous-quantitation-of-atp-and-2-3-dpg-in-human-k2edta-whole-blood
#11
Hyeryun Kim, Penelope Kosinski, Charles Kung, Lenny Dang, Yue Chen, Hua Yang, Yuan-Shek Chen, Jordyn Kramer, Guowen Liu
Many hemolytic anemias results in major metabolic abnormalities: two common metabolite abnormalities include increased levels of 2,3-diphosphoglycerate (2,3-DPG) and decreased levels of adenosine triphosphate (ATP). To better monitor the concentration changes of these metabolites, the development of a reliable LC-MS/MS method to quantitatively profile the concentrations of 2, 3-DPG and ATP in whole blood is essential to understand the effects of investigational therapeutics. Accurate quantification of both compounds imposes great challenges to bioanalytical scientists due to their polar, ionic and endogenous nature...
July 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28711614/antioxidant-and-antibacterial-activities-of-polysaccharides-isolated-and-purified-from-diaphragma-juglandis-fructus
#12
Qingran Meng, Yinghao Li, Tiancun Xiao, Lianfu Zhang, Dan Xu
A water-soluble polysaccharide fraction (DJP-2) isolated from Diaphragma juglandis was successfully purified by ion-exchange chromatography (DEAE-cellulose) and gel-permeation chromatography (Sephadex G-100). The weight-average molecular weight (Mw) and number-average molecular weight (Mn) of DJP-2 were 4.95 and 3.99kDa, respectively. Monosaccharide component analysis indicated that DJP-2 comprised arabinose, galactose, glucose, xylose, and mannose in a molar ratio of 0.27:0.55:1:0.14:0.08. The evaluation of the antioxidant and antibacterial activities of polysaccharides from Diaphragma juglandis fructus indicated that they could be explored as promising natural antioxidant and bacteriostatic agents in the food and pharmaceutical industries...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28711220/analysis-of-small-molecule-drugs-excipients-and-counter-ions-in-pharmaceuticals-by-capillary-electromigration-methods-recent-developments
#13
REVIEW
Qingfu Zhu, Gerhard K E Scriba
Capillary electromigration techniques have become important tools for the analysis of pharmaceutical drugs. The present review updates on recent developments of the analysis of small molecule drugs, excipients and counter ions dating between January 2014 and May 2017. Capillary electrophoresis (CE) continues to be a very popular method for the analysis of the active pharmaceutical ingredients and their related substances in pharmaceutical formulations, especially for the determination of the stereoisomeric purity of chiral drugs, which is evidenced by a large number of related literature reported in the reviewed period of time...
June 29, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28710734/multi-class-determination-of-pharmaceuticals-in-wastewaters-by-solid-phase-extraction-and-liquid-chromatography-tandem-mass-spectrometry-with-matrix-effect-study
#14
Mirta Čizmić, Sandra Babić, Marija Kaštelan-Macan
In this work, a multi-class analytical method for determination of 22 frequently used pharmaceuticals was developed and validated. Analytes were from different classes for example macrolides, fluoroquinolones, tetracyclines, sulfonamides, anthelmintics, anesthetics, and others. Method was intended for analysis of aqueous samples so the sample preparation was done using solid-phase extraction (SPE). Different sorbents (C8, C18, polymeric, and ion exchange sorbents) combining different eluents (methanol, ethanol, acetonitrile, acetone, ethyl acetate) were investigated during development of sample preparation step...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28709649/analysis-of-oxylipins-in-human-plasma-comparison-of-ultrahigh-performance-liquid-chromatography-and-ultrahigh-performance-supercritical-fluid-chromatography-coupled-to-mass-spectrometry
#15
Róbert Berkecz, Miroslav Lísa, Michal Holčapek
The potential of ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI-MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric interferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipins within 13min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8min...
June 29, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28709014/a-rapid-liquid-chromatography-tandem-mass-spectrometry-for-the-quantification-of-fosfomycin-in-plasma-urine-and-aqueous-fluids
#16
Nahed El-Najjar, Jonathan Jantsch, André Gessner
A simple and fast ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the analysis of Fosfomycin in different matrices (human plasma/urine, and aqueous fluid) has been established and validated. Sample cleanup consists, depending on the matrix used, on protein precipitation or dilution with methanol containing isotopically labeled Fosfomycin as internal standard. Compounds, separated on a Luna Omega PS C18 column, were detected in multiple reactions monitoring in negative ion mode using API4000 system...
June 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28708940/the-street-like-synthesis-of-krokodil-results-in-the-formation-of-an-enlarged-cluster-of-known-and-new-morphinans-with-therapeutic-potential
#17
José Soares, Emanuele Alves, André Silva, Natália Figueiredo, João F Neves, Sara Cravo, Maria Rangel, Annibal Netto, Félix Carvalho, Ricardo Jorge Dinis-Oliveira, Carlos Afonso
"Krokodil" is the street name for a homemade injectable drug that has been used as a cheap substitute for heroin. Codeine is the opioid starting material for krokodil synthesis and desomorphine is the opioid claimed to be the main component of krokodil and the main responsible for its addictive and psychoactive characteristics. However, due to its peculiar manufacture, using cheap raw materials, krokodil is composed by a large and complex mixture of different substances. In order to shed some light upon the chemical complexity of krokodil, its profiling was conducted by reverse phase high performance liquid chromatography coupled to photodiode array detector (RP-HPLC-DAD) and by liquid chromatography coupled to high resolution tandem mass spectrometry (LC-ESI-IT-Orbitrap-MS)...
July 14, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28708331/negative-chemical-ionization-gas-chromatography-mass-spectrometry-of-transfluthrin-in-rat-plasma-and-brain
#18
Shirin Hooshfar, Tanzir B Mortuza, Clinton A Rogers, Michael R Linzey, Darren R Gullick, James V Bruckner, Catherine A White, Michael G Bartlett
RATIONALE: Transfluthrin is a relatively non-toxic rapid-acting synthetic pyrethroid insecticide. It is widely used in household and hygiene products. A sensitive and accurate bioanalytical method is required for quantification of its concentration in plasma and its potential target organ, the brain for studies to assess its health effects and toxicokinetics in mammals. METHODS: The samples were prepared by liquid-liquid extraction. Gas chromatography mass spectrometry (GC/MS) analysis was performed for the determination of transfluthrin in biological samples with an overall method run time of 15 min...
July 14, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28708288/distinguishing-of-2-mapb-and-6-mapb-solution-of-the-problem
#19
Vadim Shevyrin, Yuri Shafran
Differentiation of new psychoactive substance (NPS), 6-(2-methylaminopropyl)benzofuran (6-MAPB), and its positional isomer, 2-(2-methylaminopropyl)benzofuran (2-MAPB), by means of gas chromatography/mass spectrometry (GC/MS) with quadrupole detection is ambiguous. Reliable distinguishing of the two isomers could be achieved by MS/MS spectra recorded after collision-induced dissociation (CID) of precursor ions. Both electron ionization (EI) and electrospray ionization (ESI) methods could be used for these purposes...
July 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28708032/comparison-of-a-newly-developed-liquid-chromatography-with-tandem-mass-spectrometry-method-and-enzyme-linked-immunosorbent-assay-for-detection-of-multiple-mycotoxins-in-red-pepper-powder
#20
Sunyoung Kim, Sanghee Lee, Tae-Gyu Nam, Dongwon Seo, Miyoung Yoo
In this study, we aimed to establish a method for determination of multiple mycotoxins such as aflatoxins, ochratoxin A, and zearalenone in red pepper powder samples based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for extraction and cleanup, with detection and quantification by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in both positive- and negative-ion modes. The developed LC-MS/MS analytical method was compared with an enzyme-linked immunosorbent assay (ELISA) to improve the reliability of our developed method...
July 14, 2017: Journal of Food Protection
keyword
keyword
97204
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"