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https://www.readbyqxmd.com/read/28436222/influence-of-nitric-acid-on-the-helium-ion-radiolysis-of-aqueous-butanal-oxime-solutions
#1
Amaury Costagliola, Laurent Venault, Arnaud Deroche, Jackie Vermeulen, Florian Duval, Guillaume Blain, Johan Vandenborre, Massoud Fattahi-Vanani, Nicolas Vigier
Samples of butanal oxime in aqueous nitric acid solutions have been irradiated with the helium ion (4He2+) beam of the CEMHTI (Orléans, France) cyclotrons. The consumption yield of butanal oxime has been measured by gas-chromatography coupled with mass spectrometry. Gaseous products (mainly H2 and N2O) have also been monitored by micro gas-chromatography. Yields of liquid phase products (hydrogen peroxide and nitrous acid) have been determined by colorimetric methods. The influence of nitric acid on the radiation chemical behavior of butanal oxime depends on the nitric acid concentration...
April 24, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28434713/detection-of-trace-fluoride-in-serum-and-urine-by-online-membrane-based-distillation-coupled-with-ion-chromatography
#2
Chaoyan Lou, Dandan Guo, Nani Wang, Shuchao Wu, Peimin Zhang, Yan Zhu
An online membrane-based distillation (MBD) coupled with ion chromatography (IC) method was proposed for automatic detection of trace fluoride (F(-)) in serum and urine samples. The system consisted of a sample vessel, a lab-made membrane module and an ion chromatograph. Hydrophobic polytetrafluoroethylene (PTFE) hollow fiber membrane was used in MBD which was directly performed in serum and urine samples to eliminate the matrix interferences and enrich fluoride, while enabling automation. The determination of fluoride in biological samples was carried out by IC with suppressed conductometric detection...
April 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28434712/evidence-for-an-imidazoline-by-product-from-glycans-using-tandem-mass-spectrometry
#3
Robert J Duff, Elaine Smith, Wenzhou Li, Szilan Fodor
Herein is reported the separation and identification of a previously unknown imidazoline by-product originating from the fluorescent labeling procedure when applied to enzymatically released N-linked glycans of a human IgG1. The imidazoline by-product was generated via the reductive amination procedure with either sodium cyanoborohydride or 2-picoline borane. Using ultra performance liquid chromatography (UPLC) in conjunction with hydrophilic interaction-based chromatography (HILIC), the 2-aminobenzoic acid (2-AA)-labeled glycans were well-resolved from imidazoline by-products to facilitate direct identification utilizing electrospray ionization mass spectrometry (ESI-MS) with fragmentation...
April 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28434709/target-analysis-of-tert-butyldimethylsilyl-derivatives-of-nerve-agent-hydrolysis-products-by-selectable-one-dimensional-or-two-dimensional-gas-chromatography-mass-spectrometry
#4
Yasuo Seto, Masumi Tachikawa, Mieko Kanamori-Kataoka, Kikuo Sasamoto, Nobuo Ochiai
A target analysis method for the sensitive and discriminative determination of the nerve agent hydrolysis products alkyl methylphosphonic acids as their tert-butyldimethylsilyl (TBDMS) derivatives was developed using a combination of selectable one- and two-dimensional ((1)D/(2)D) GC-MS, and applied to the analysis of samples with significant interfering matrices. After sample drying, the alkylmethylphosphonic acids and methylphosphonic acid (MPA) were converted to TBDMS derivatives by addition of N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide with heating, and subjected to (1)D/(2)D GC-MS...
April 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28434171/peroxidatic-cysteine-residue-of-peroxiredoxin-2-separated-from-human-red-blood-cells-treated-by-tert-butyl-hydroperoxide-is-hyperoxidized-into-sulfinic-and-sulfonic-acids
#5
Yo-Ichi Ishida, Mariko Aki, Sohta Fujiwara, Masami Nagahama, Yuki Ogasawara
Peroxiredoxin 2 (Prx2) is a redox enzyme that is abundantly expressed in red blood cells (RBCs) and has been the focus of clinical attention for monitoring the oxidative status. We previously developed a method to quantify the reduced and hyperoxidized forms of Prx2 in human RBCs using reverse-phase high-performance liquid chromatography (HPLC). In the present study, we investigated the hyperoxidative status of Prx2 at the molecular level in a post-translational modification analysis using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system...
April 22, 2017: Human Cell
https://www.readbyqxmd.com/read/28433954/lipidomic-profile-in-three-species-of-dinoflagellates-amphidinium-carterae-cystodinium-sp-and-peridinium-aciculiferum-containing-very-long-chain-polyunsaturated-fatty-acids
#6
Tomáš Řezanka, Jaromír Lukavský, Linda Nedbalová, Karel Sigler
This study describes the identification of very long chain polyunsaturated fatty acids (VLCPUFAs) in three strains of dinoflagellates (Amphidinium carterae, Cystodinium sp., and Peridinium aciculiferum). The strains were cultivated and their lipidomic profiles were obtained by high resolution mass spectrometry with the aid of positive and negative electrospray ionization (ESI) mode by Orbitrap apparatus. Hydrophilic interaction liquid chromatography (HILIC/ESI) was used to separate major lipid classes of the three genera of dinoflagellates by neutral loss scan showing the ion [M + H-28:8](+), where 28:8 was octacosaoctaenoic acid, and by precursor ion scanning of ions at m/z 407, which was an ion corresponding to the structure of acyl of 28:8 acid (C27H39COO(-))...
April 20, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28433872/biosynthesis-of-silver-nanoparticles-by-using-camellia-japonica-leaf-extract-for-the-electrocatalytic-reduction-of-nitrobenzene-and-photocatalytic-degradation-of-eosin-y
#7
R Karthik, Mani Govindasamy, Shen-Ming Chen, Yi-Hui Cheng, P Muthukrishnan, S Padmavathy, A Elangovan
In the present study, sphere-like silver nanoparticles (Ag-NPs) were synthesized by using Camellia japonica leaf extract and its remediation industrial pollutants such as nitrobenzene and Eosin-Y (EY). As-prepared sphere-like Ag-NPs were characterized by various analytical and spectroscopic methods such as UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), High-resolution transmission electron microscopy (HR-TEM), Energy dispersive X-ray spectra (EDX), and the chemical constituents of the leaf extract were also analyzed by using Gas chromatography and Mass Spectroscopy (GC-MS)...
March 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28433761/a-glycoproteomic-approach-reveals-that-the-s-layer-glycoprotein-of-lactobacillus-kefiri-cidca-83111-is-o-and-n-glycosylated
#8
Gustavo J Cavallero, Mariano Malamud, Adriana C Casabuono, M de Los Ángeles Serradell, Alicia S Couto
In Gram-positive bacteria, such as lactic acid bacteria, general glycosylation systems have not been documented so far. The aim of this work was to characterize in detail the glycosylation of the S-layer protein of Lactobacillus kefiri CIDCA 83111. A reductive β-elimination treatment followed by anion exchange high performance liquid chromatography analysis was useful to characterize the O-glycosidic structures. MALDI-TOF mass spectrometry analysis confirmed the presence of oligosaccharides bearing from 5 to 8 glucose units carrying galacturonic acid...
April 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28433749/novel-sulphur-containing-imatinib-metabolites-found-by-untargeted-lc-hrms-analysis
#9
Ivo Vrobel, David Friedecký, Edgar Faber, Lukáš Najdekr, Kateřina Mičová, Radana Karlíková, Tomáš Adam
Untargeted metabolite profiling using high-resolution mass spectrometry coupled with liquid chromatography (LC-HRMS), followed by data analysis with the Compound Discoverer 2.0™ software, was used to study the metabolism of imatinib in humans with chronic myeloid leukemia. Plasma samples from control (drug-free) and patient (treated with imatinib) groups were analyzed in full-scan mode and the unknown ions occurring only in the patient group were then, as potential imatinib metabolites, subjected to multi-stage fragmentation in order to elucidate their structure...
April 19, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28433609/hb-moncloa-a-new-variant-of-haemoglobin-that-interferes-in-the-quantification-of-hb-a1c
#10
Torrejón María Josefa, Ortíz-Cabrera N Valentina, M Nieto Jorge, González Fernández Fernando-Ataúlfo, Villegas Ana, Martínez Rafael, Ropero Paloma
BACKGROUND: In most routine laboratories in Spain, the commonly used method for evaluating HbA1c is ion-exchange high performance liquid chromatography (HPLC). The presence of a variant of Hb may interfere with the quantification of HbA1c. AIMS: Here, we report a novel haemoglobin variant, named Hb Moncloa, which was found during a routine health check at the Hospital Clínico San Carlos in Moncloa (Madrid), Spain. METHODS AND RESULTS: Molecular characterization of β gene identified a novel transversion mutation [β80(EF4)Asn>Ser; HBB:c...
April 19, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28433551/in-vitro-investigation-of-metabolic-profiling-of-newly-developed-topoisomerase-inhibitors-ethyl-fluorescein-hydrazones-etflhs-in-rlms-by-lc-ms-ms
#11
Adnan A Kadi, Nasser S Al-Shakliah, Wencui Yin, A F M Motiur Rahman
Metabolic profiling of newly reported five topoisomerase inhibitors namely ethyl fluorescein hydrazones (EtFLHs) were studied in rat liver microsomes (RLMs) and the data were acquired in a liquid chromatography (LC) ion trap mass spectrometry. Hydroxyl group containing EtFLHs derivatives (1-3) were bio-transformed into hydrolyzed, mono-hydroxylated and hydrolyzed together with mono-hydroxylated metabolites. On the other hand, nitro and methoxy groups containing EtFLHs derivatives (4-5) were bio-transformed into hydrolyzed, hydrolyzed together with mono-hydroxylated and azo-reductive metabolites in the presence of NADPH...
April 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28433061/graphene-coated-polymeric-anion-exchangers-for-ion-chromatography
#12
Kai Zhang, Minyi Cao, Chaoyan Lou, Shuchao Wu, Peimin Zhang, Mingyu Zhi, Yan Zhu
Carbonaceous stationary phases have gained much attention for their peculiar selectivity and robustness. Herein we report the fabrication and application of a graphene-coated polymeric stationary phase for anion exchange chromatography. The graphene-coated particles were fabricated by a facile evaporation-reduction method. These hydrophilic particles were proven appropriate substrates for grafting of hyperbranched condensation polymers (HBCPs) to make pellicular anion exchangers. The new phase was characterized by zeta potentials, Fourier transform infrared spectroscopy, thermogravimetry and scanning electron microscope...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28432915/proton-induced-production-and-radiochemical-isolation-of-44-ti-from-scandium-metal-targets-for-44-ti-44-sc-generator-development
#13
Valery Radchenko, Jonathan W Engle, Dmitri G Medvedev, Joel M Maassen, Cleo M Naranjo, George A Unc, Catherine A L Meyer, Tara Mastren, Mark Brugh, Leonard Mausner, Cathy S Cutler, Eva R Birnbaum, Kevin D John, F Meiring Nortier, Michael E Fassbender
Scandium-44g (half-life 3.97h) shows promise for application in positron emission tomography (PET), due to favorable decay parameters. One of the sources of (44g)Sc is the (44)Ti/(44g)Sc generator, which can conveniently provide this radioisotope on a daily basis at a diagnostic facility. Titanium-44 (half-life 60.0 a), in turn, can be obtained via proton irradiation of scandium metal targets. A substantial (44)Ti product batch, however, requires high beam currents, long irradiation times and an elaborate chemical procedure for (44)Ti isolation and purification...
April 7, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28431324/achievable-separation-performance-and-analysis-time-in-current-liquid-chromatographic-practice-for-monoclonal-antibody-separations
#14
Szabolcs Fekete, Jean-Luc Veuthey, Davy Guillarme
The separation performance of a chromatographic system is often described in terms of column efficiency and peak capacity. Thanks to the new developments in column technology over the past few years, the achievable peak capacity drastically improved and the analysis time can be significantly shortened. Indeed, highly efficient wide-pore reversed-phase (RPLC) materials packed with small fully porous and superficially porous particles can be successfully used for the analytical characterization of therapeutic proteins...
April 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28431006/regulation-of-oxidized-platelet-lipidome-implications-for-coronary-artery-disease
#15
Madhumita Chatterjee, Dominik Rath, Jörg Schlotterbeck, Johannes Rheinlaender, Britta Walker-Allgaier, Nada Alnaggar, Monika Zdanyte, Iris Müller, Oliver Borst, Tobias Geisler, Tilman E Schäffer, Michael Lämmerhofer, Meinrad Gawaz
Aims: Hyperlipidaemia enhances susceptibility to thrombosis, while platelet oxidixed LDL (oxLDL) binding in acute coronary syndrome (ACS) correlates with activation status. This study explores the platelet lipidome in symptomatic coronary artery disease (CAD) patients and the functional consequences of the chemokine CXCL12 and its receptors CXCR-4/-7 on lipid uptake in platelets. Methods and results: Platelet-oxLDL detected by flow cytometry was enhanced (P = 0...
April 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28429592/peptide-retention-time-prediction-in-hydrophilic-interaction-liquid-chromatography-data-collection-methods-and-features-of-additive-and-sequence-specific-models
#16
Oleg V Krokhin, Peyman Ezzati, Vic Spicer
The development of a peptide retention prediction model for hydrophilic interaction liquid chromatography (XBridge Amide column) is described for a collection of ~40,000 tryptic peptides. Off-line 2D LC-MS/MS analysis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention information for a HILIC separation. The large size of the optimization dataset (more than two orders of magnitude compared to previous reports) permits the accurate assignment of hydrophilic retention coefficients of individual amino acids, establishing both, the effects of amino-acid position relative to peptide termini and the influence of peptide secondary structure in HILIC...
April 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28428354/multiplex-tandem-mass-spectrometry-enzymatic-activity-assay-for-newborn-screening-of-the-mucopolysaccharidoses-and-type-2-neuronal-ceroid-lipofuscinosis
#17
Yang Liu, Fan Yi, Arun Babu Kumar, Naveen Kumar Chennamaneni, Xinying Hong, C Ronald Scott, Michael H Gelb, Frantisek Turecek
BACKGROUND: We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL). METHODS: New substrates were prepared and char-acterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB)...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28427917/application-of-the-isoniazid-assay-in-dried-blood-spots-using-the-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#18
Kyunghoon Lee, Sun-Hee Jun, Moon-Suk Choi, Sang Hoon Song, Jong Sun Park, Jae Ho Lee, Kyoung Un Park, Junghan Song
OBJECTIVES: Therapeutic drug monitoring (TDM) of anti-tuberculosis (TB) drugs is important for proper treatment of TB. Dried blood spots (DBSs) are widely used for TDM because of their several advantages. Rifampicin and pyrazinamide assays with DBSs have already been developed. However, isoniazid (INH) assay for capillary DBSs have not been reported because of INH instability. We developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for measuring INH concentrations in venous and capillary DBSs...
April 17, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28427113/simultaneous-quantification-and-semi-quantification-of-amentoflavone-and-its-metabolites-in-human-intestinal-bacteria-by-liquid-chromatography-orbitrap-high-resolution-mass-spectrometry
#19
Yiyun Qian, Shu Jiang, Zhenhua Zhu, Qi Wang, Shulan Su, Jinhua Tao, Jin-Ao Duan
A quick, easy, effective method followed by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed for the simultaneous identification and quantification of the metabolites produced by Amentoflavone (AMF) in human intestinal bacteria from human feces. The method validated for quantification of AMF concerning precision, accuracy, recovery, matrix effect, stability and limits showed acceptable results. Compared the blank human intestinal bacteria chromatography, three metabolites were presumed based on high-accuracy protonated precursors and multi-stage mass spectrometry (MS(n) ) using the proposed strategy...
April 20, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28425545/beware-of-phosphate-evidence-of-specific-dendrimer-phosphate-interactions
#20
Mónika Kéri, Zoltán Nagy, Levente Novák, Edit Szarvas, Lajos P Balogh, István Bányai
Dendrimers are extensively studied for drug delivery and catalysis, most of which are pH dependent. Phosphate buffer solutions (PBSs) are often used to adjust the pH. We have found that phosphate ions become incorporated into poly(amidoamine) (PAMAM) dendrimer molecules by forming H-bonds with tertiary nitrogens. We show that this specific interaction between H2PO4(-) and HPO4(2-) ions and generation five PAMAM dendrimers causes a decrease in hydrodynamic size, disturbing the outcome of the size exclusion chromatography analysis...
April 20, 2017: Physical Chemistry Chemical Physics: PCCP
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