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Mesenchymal sten cell

Eleni Chantzoura, Stavroula Skylaki, Sergio Menendez, Shin-Il Kim, Anna Johnsson, Sten Linnarsson, Knut Woltjen, Ian Chambers, Keisuke Kaji
Since the first generation of induced pluripotent stem cells (iPSCs), several reprogramming systems have been used to study its molecular mechanisms. However, the system of choice largely affects the reprogramming efficiency, influencing our view on the mechanisms. Here, we demonstrate that reprogramming triggered by less efficient polycistronic reprogramming cassettes not only highlights mesenchymal-to-epithelial transition (MET) as a roadblock but also faces more severe difficulties to attain a pluripotent state even post-MET...
September 8, 2015: Stem Cell Reports
David H Engelsvold, Tor P Utheim, Ole K Olstad, Pedro Gonzalez, Jon R Eidet, Torstein Lyberg, Anne-Marie S Trøseid, Darlene A Dartt, Sten Raeder
The current study investigates whether microRNA (miRNA) regulators of epithelial-mesenchymal transition (EMT), tissue fibrosis, and angiogenesis are differentially expressed in human primary pterygium. Genome-wide miRNA and mRNA expression profiling of paired pterygium and normal conjunctiva was performed in the context of conventional excision of pterygium with autotransplantation of conjunctiva (n = 8). Quantitative real time polymerase chain reaction (qRT-PCR) was used to validate the expression of key molecules previously detected by microarray...
October 2013: Experimental Eye Research
Kersti Jääger, Saiful Islam, Pawel Zajac, Sten Linnarsson, Toomas Neuman
BACKGROUND: Tissue regeneration and recovery in the adult body depends on self-renewal and differentiation of stem and progenitor cells. Mesenchymal stem cells (MSCs) that have the ability to differentiate into various cell types, have been isolated from the stromal fraction of virtually all tissues. However, little is known about the true identity of MSCs. MSC populations exhibit great tissue-, location- and patient-specific variation in gene expression and are heterogeneous in cell composition...
2012: PloS One
Anna Mansour, Grazia Abou-Ezzi, Ewa Sitnicka, Sten Eirik W Jacobsen, Abdelilah Wakkach, Claudine Blin-Wakkach
Formation of the hematopoietic stem cell (HSC) niche in bone marrow (BM) is tightly associated with endochondral ossification, but little is known about the mechanisms involved. We used the oc/oc mouse, a mouse model with impaired endochondral ossification caused by a loss of osteoclast (OCL) activity, to investigate the role of osteoblasts (OBLs) and OCLs in the HSC niche formation. The absence of OCL activity resulted in a defective HSC niche associated with an increased proportion of mesenchymal progenitors but reduced osteoblastic differentiation, leading to impaired HSC homing to the BM...
March 12, 2012: Journal of Experimental Medicine
You-Ying Chau, David Brownstein, Heidi Mjoseng, Wen-Chin Lee, Natalija Buza-Vidas, Claus Nerlov, Sten Eirik Jacobsen, Paul Perry, Rachel Berry, Anna Thornburn, David Sexton, Nik Morton, Peter Hohenstein, Elisabeth Freyer, Kay Samuel, Rob van't Hof, Nicholas Hastie
There is much interest in the mechanisms that regulate adult tissue homeostasis and their relationship to processes governing foetal development. Mice deleted for the Wilms' tumour gene, Wt1, lack kidneys, gonads, and spleen and die at mid-gestation due to defective coronary vasculature. Wt1 is vital for maintaining the mesenchymal-epithelial balance in these tissues and is required for the epithelial-to-mesenchyme transition (EMT) that generates coronary vascular progenitors. Although Wt1 is only expressed in rare cell populations in adults including glomerular podocytes, 1% of bone marrow cells, and mesothelium, we hypothesised that this might be important for homeostasis of adult tissues; hence, we deleted the gene ubiquitously in young and adult mice...
December 2011: PLoS Genetics
Ariane Tormin, Jan C Brune, Eleonor Olsson, Jeanette Valcich, Ulf Neuman, Tor Olofsson, Sten-Eirik Jacobsen, Stefan Scheding
BACKGROUND AIMS: Human mesenchymal stromal cells (MSC) are promising candidates for cell therapy because of their intriguing properties (high proliferation and differentiation capacity, microenvironmental function and immune modulation). However, MSC are heterogeneous and a better understanding of the heterogeneity of the cells that form the MSC cultures is critical. METHODS: Human MSC were generated in standard cultures and stained with carboxyfluorescein succinimidyl ester (CFSE) for cell division tracking...
2009: Cytotherapy
Martin Breitbach, Toktam Bostani, Wilhelm Roell, Ying Xia, Oliver Dewald, Jens M Nygren, Jochen W U Fries, Klaus Tiemann, Heribert Bohlen, Juergen Hescheler, Armin Welz, Wilhelm Bloch, Sten Eirik W Jacobsen, Bernd K Fleischmann
Cellular replacement therapy has emerged as a novel strategy for the treatment of heart failure. The aim of our study was to determine the fate of injected mesenchymal stem cells (MSCs) and whole bone marrow (BM) cells in the infarcted heart. MSCs were purified from BM of transgenic mice and characterized using flow cytometry and in vitro differentiation assays. Myocardial infarctions were generated in mice and different cell populations including transgenic MSCs, unfractionated BM cells, or purified hematopoietic progenitors were injected...
August 15, 2007: Blood
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