Read by QxMD icon Read

Pharmaceutical microbiology

Ana Santoveña, Irma Charola, Javier Suárez-González, Nuria Teigell-Pérez, Susana García-van Nood, Mabel Soriano, José B Fariña
There is as yet no commercialized preparation for oral administration of flecainide acetate (FA) to children. In such cases, manipulation of commercial tablets is the usual practice in pharmacy services of hospitals and compounding pharmacies, to provide a suitable dosage form for this vulnerable pediatric population group. In this study we have formulated FA as an oral solution, as an alternative to the suspension elaborated from commercial tablets. Due to this sensitivity of young patients, we have used the pure active pharmaceutical ingredient (API) and the lowest permitted levels of pediatric excipients...
September 23, 2016: Pharmaceutical Development and Technology
Vinicio Carias, Zohreh Nemati Porshokouh, Kristen Stojak Repa, Javier Alonso, Hariharan Srikanth, Jürgen Rühe, Ryan Toomey, Jing Wang
The micromanipulation of biological samples is important for microbiology, pharmaceutical science, and related bioengineering fields. In this work, we report the fabrication and characterization of surface-attached microbeam arrays of 20 μm width and 25 μm height made of poly(N-isopropylacrylamide), a thermoresponsive polymer, with embedded spherical or octopod Fe3O4 nanoparticles. Below 32 °C, the microbeams imbibe water and buckle with an amplitude of approximately 20 μm. Turning on an AC-magnetic field induces the microbeam array to expel water due to the heating effect of the nanoparticles (magnetic hyperthermia), leading to a reversible transition from a buckled to nonbuckled state...
October 5, 2016: ACS Applied Materials & Interfaces
Guinet Marie Frédéric Roland, Berthoumieu Nicole, Dutot Philippe, Triquet Julien, Ratajczak Mehdi, Thibaudon Michel, Bechaud Philippe, Arliaud Christophe, Miclet Edith, Giordano Florine, Larcon Marjorie, Arthaud Catherine
Environmental monitoring and aseptic process simulations represent an integral part of the microbiological quality control system of sterile pharmaceutical products manufacturing operations. However, guidance documents and manufacturers practices differ regarding recommendation for incubation time and incubation temperature and, consequently, the environmental monitoring and aseptic process simulation incubation strategy should be supported by validation data. To avoid any bias coming from in vitro studies or from single manufacturing in situ site studies, we performed a collaborative study at four manufacturing sites with four samples at the same location...
September 4, 2016: PDA Journal of Pharmaceutical Science and Technology
Marcel Goverde, Julian Willrodt, Alexandra Staerk
Contact plates, dipslides and swabs are used for the microbiological monitoring of surfaces in controlled environments such as pharmaceutical clean rooms. In the present study, three different swab types using two different methods (direct streaking on agar versus elution followed by membrane filtration) were evaluated. In a first study, representative surfaces in pharmaceutical clean rooms were artificially inoculated using three different environmental strains (in vitro study). In a second study, a naturally inoculated floor was swabbed with the same three swab types, again using the two different recovery methods (in situ study)...
August 11, 2016: PDA Journal of Pharmaceutical Science and Technology
Filipa C Silva, Joana Marto, Ana Salgado, Paula Machado, Alexandra Silva, António J Almeida
Oral mucositis is a common adverse reaction to radiotherapy and chemotherapy in oncology. Its treatment requires oral formulations that enhance therapy compliance, improve administration and ensure drug effectiveness. Solid dosage forms that act by slow dissolution, such as pastilles, are an effective alternative to mouthwashes, for their versatility, ease of administration and extended residence time in the oral cavity. The present work describes the development and stability studies of an innovative formulation of nystatin and lidocaine pastilles for the treatment of oral mucositis...
August 8, 2016: Pharmaceutical Development and Technology
Changting Liu
Microorganisms exhibit high adaptability to extreme environments of outer space via phenotypic and genetic changes. These changes may affect astronauts in the space environment as well as on Earth because mutant microbes will inevitably return with the spacecraft. But the role and significance of these phenotypic changes and the underlying mechanisms are important unresolved questions in the field of space biology. By reviewing, especially the Chinese studies, we propose a space microbial molecular effect theory, that is, the space environment affects the nature of genes and the molecular structure of microorganisms to produce phenotypic changes...
July 26, 2016: Environmental Microbiology
G V Tetz, V V Tetz, T M Voroshilova, E I Smirnova, K M Kardava, T A Karamian, N V Zaslavskaia, D S Vikina, M A Zaitseva, K M Artemenko, A S Kaufman
The effectiveness of application of test-system "Choice of antibiotic" was evaluated as a tool for incubation of maximal amount of bacteria from pathological material under acute cystitis. The results of meta-genome analysis established that test-system permits supporting growth of practically all bacteria detected in urine, including ones relating to "uncultivated for the present". The comparison of results of standard detection of sensitivity of bacteria to antibiotics and identification of effective pharmaceutical according the results of application of test-system "Choice of antibiotic" as well was implemented It is demonstrated that test- system permits choosing antibiotic during 6-20 hours wiihout isolation of pure strain...
February 2016: Klinicheskaia Laboratornaia Diagnostika
Piotr Jarocki, Marcin Podleśny, Elwira Komoń-Janczara, Jagoda Kucharska, Agnieszka Glibowska, Zdzisław Targoński
BACKGROUND: Members of the genus Bifidobacterium are anaerobic Gram-positive Actinobacteria, which are natural inhabitants of human and animal gastrointestinal tract. Certain bifidobacteria are frequently used as food additives and probiotic pharmaceuticals, because of their various health-promoting properties. Due to the enormous demand on probiotic bacteria, manufacture of high-quality products containing living microorganisms requires rapid and accurate identification of specific bacteria...
2016: BMC Microbiology
Hideharu Shintani
Microbial testing performed in support of pharmaceutical and biopharmaceutical production falls into three main categories: detection (qualitative), enumeration (quantitative), and characterization/identification. Traditional microbiological methods are listed in the compendia and discussed by using the conventional growth-based techniques, which are labor intensive and time consuming. In general, such tests require several days of incubation for microbial contamination (bioburden) to be detected, and therefore management seldom is able to take proactive corrective measures...
2016: Biocontrol Science
J Marto, L F Gouveia, L Gonçalves, B G Chiari-Andréo, V Isaac, P Pinto, E Oliveira, A J Almeida, H M Ribeiro
Green coffee oil and modified starch were recently found to have an enhanced protection effect against UV radiation. Therefore, this work aimed to develop an innovative sunscreen formulation based on Pickering emulsions concept, i.e., surfactant-free emulsions stabilized by physical UV filters associated natural oils as a key strategy for prevention against UV-induced skin damage. The Pickering emulsions of different compositions were characterized in terms of pH, mechanical, physical and microbiological stability by a thorough pharmaceutical control...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
Sonja Gvozdenac, Vojislava Bursić, Gorica Vuković, Simonida Đurić, Carlos Gonçalves, Dušica Jovičić, Snežana Tanasković
Irrigation is one of the most important uses of surface waters in the agricultural region of Vojvodina province (Serbia). The aim of the study was to assess the quality of water from Stara Tisa meander, based on the levels of pollution with metals, volatile compounds (VOC), pharmaceuticals, pesticides, and pathogenic bacteria, on sunflower, cabbage, cucumber, maize, barley, buckwheat, sorghum, radish, beans, and white mustard. Microbiological analysis was carried out using the dilution method and phytotoxicity assay according to ISTA filter paper method (germination energy (GE), germination (G), root and shoot length, fresh and dry weight)...
September 2016: Environmental Science and Pollution Research International
Paweł Olczyk, Robert Koprowski, Justyna Kaźmierczak, Lukasz Mencner, Robert Wojtyczka, Jerzy Stojko, Krystyna Olczyk, Katarzyna Komosinska-Vassev
The aim of the present study was to visualize the benefits and advantages derived from preparations based on extracts of bee pollen as compared to pharmaceuticals commonly used in the treatment of burns. The bee pollen ointment was applied for the first time in topical burn treatment. Experimental burn wounds were inflicted on two white, domestic pigs. Clinical, histopathological, and microbiological assessment of specimens from burn wounds, inflicted on polish domestic pigs, treated with silver sulfadiazine or bee pollen ointment, was done...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Nelson Durán, Gerson Nakazato, Amedea B Seabra
The antimicrobial impact of biogenic-synthesized silver-based nanoparticles has been the focus of increasing interest. As the antimicrobial activity of nanoparticles is highly dependent on their size and surface, the complete and adequate characterization of the nanoparticle is important. This review discusses the characterization and antimicrobial activity of biogenic synthesized silver nanoparticles and silver chloride nanoparticles. By revising the literature, there is confusion in the characterization of these two silver-based nanoparticles, which consequently affects the conclusion regarding to their antimicrobial activities...
August 2016: Applied Microbiology and Biotechnology
Claudia Loewe, Jens Atzrodt, Kai Reschke, Joe Schofield
The human absorption, distribution, metabolism and elimination study administering radiolabeled drugs to human volunteers is an important clinical study in the development program of new drug candidates. The manufacture of radiolabeled Active Pharmaceutical Ingredients is covered by national drug laws and may come within the scope of regulatory GMP requirements. Additionally, authorities may request an appropriate environmental zoning to minimize the risk of microbiological contaminations particularly during the synthesis of radiolabeled Active Pharmaceutical Ingredients intended for parenteral application...
June 10, 2016: Journal of Labelled Compounds & Radiopharmaceuticals
Motayo Babatunde Olanrewaju, Akingbade Olusola, Nwadike Victor, Shobayo Olabode, Ogiogwa Joseph, Akinduti Akiniyi, Okonko Iheanyi
The recent introduction of the Heptavalent-pneumococcal vaccine (PCV-7) by private pharmaceutical companies in Nigeria, has generated interest in invasive bacterial diseases particularly IPD. Our objective in this study is to investigate the trend and occurrence rate of IPD in Abeokuta, Nigeria. Suspected IPD cases were assessed from Jan 2010 to Dec 2010 for demographic and Microbiological characteristics. Bacterial isolations and antibiotics susceptibility testing followed standard bacteriological procedure...
2016: Pan African Medical Journal
Borche Stamatoski, Miroslava Ilievska, Hristina Babunovska, Nikola Sekulovski, Sasho Panov
Microbiological control is of crucial importance in the pharmaceutical industry regarding the possible bacterial contamination of the environment, water, raw materials and finished products. Molecular identification of bacterial contaminants based on DNA sequencing of the hypervariable 16SrRNA gene has been introduced recently. The aim of this study is to investigate the suitability of gene sequencing using our selection of PCR primers and conditions for rapid and accurate bacterial identification in pharmaceutical industry quality control...
June 1, 2016: Acta Pharmaceutica
Katarzyna Hnatuszko-Konka, Piotr Łuchniak, Aneta Wiktorek-Smagur, Aneta Gerszberg, Tomasz Kowalczyk, Justyna Gatkowska, Andrzej K Kononowicz
Here, we present the application of microbiology and biotechnology for the production of recombinant pharmaceutical proteins in plant cells. To the best of our knowledge and belief it is one of few examples of the expression of the prokaryotic staphylokinase (SAK) in the eukaryotic system. Despite the tremendous progress made in the plant biotechnology, most of the heterologous proteins still accumulate to low concentrations in plant tissues. Therefore, the composition of expression cassettes to assure economically feasible level of protein production in plants remains crucial...
July 2016: World Journal of Microbiology & Biotechnology
H C Polonini, S L Silva, C N Cunha, M A F Brandão, A O Ferreira
A challenge with compounding oral liquid formulations is the limited availability of data to support the physical, chemical and microbiological stability of the formulation. This poses a patient safety concern and a risk for medication errors. The objective of this study was to evaluate the compatibility of the following active pharmaceutical ingredients (APIs) in 10 oral suspensions, using SyrSpend SF PH4 (liquid) as the suspending vehicle: cholecalciferol 50,000 IU/mL, haloperidol 0.5 mg/mL, imipramine hydrochloride 5...
April 2016: Die Pharmazie
Anna M Kot, Stanisław Błażejak, Agnieszka Kurcz, Iwona Gientka, Marek Kieliszek
Rhodotorula glutinis is capable of synthesizing numerous valuable compounds with a wide industrial usage. Biomass of this yeast constitutes sources of microbiological oils, and the whole pool of fatty acids is dominated by oleic, linoleic, and palmitic acid. Due to its composition, the lipids may be useful as a source for the production of the so-called third-generation biodiesel. These yeasts are also capable of synthesizing carotenoids such as β-carotene, torulene, and torularhodin. Due to their health-promoting characteristics, carotenoids are commonly used in the cosmetic, pharmaceutical, and food industries...
July 2016: Applied Microbiology and Biotechnology
Carolina Chiellini, Stefano Mocali, Renato Fani, Iolanda Ferro, Serenella Bruschi, Alessandro Pinzani
No abstract text is available yet for this article.
August 2016: Journal of Microbiological Methods
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"