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https://www.readbyqxmd.com/read/28734141/new-dinuclear-palladium-ii-complexes-studies-of-the-nucleophilic-substitution-reactions-dna-bsa-interactions-and-cytotoxic-activity
#1
Dušan Ćoćić, Snežana Jovanović, Marija Nišavić, Dejan Baskić, Danijela Todorović, Suzana Popović, Živadin D Bugarčić, Biljana Petrović
Six new dinuclear Pd(II) complexes, [{Pd(2,2'-bipy)Cl}2(μ-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}2(μ-pz)](ClO4)2 (Pd2), [{Pd(en)Cl}2(μ-pz)](ClO4)2 (Pd3), [{Pd(2,2'-bipy)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd4), [{Pd(dach)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd5) and [{Pd(en)Cl}2(μ-4,4'-bipy)](ClO4)2 (Pd6) (where 2,2'-bipy=2,2'-bipyridyl, pz=pyrazine, dach=trans-(±)-1,2-diaminocyclohexane, en=ethylenediamine, 4,4'-bipy=4,4'-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, (1)H NMR and MALDI-TOF mass spectrometry...
July 16, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28732263/proteomic-analysis-of-naturally-occurring-boron-tolerant-plant-gypsophila-sphaerocephala-l-in-response-to-high-boron-concentration
#2
Huseyin Tombuloglu, Guzin Tombuloglu, Mehmet Serdal Sakcali, Ali Turkan, Khalid Rehman Hakeem, Hesham F Alharby, Shah Fahd, Waseem Mohammed Abdul
Gypsophila sphaerocephala is a naturally Boron (B) tolerant species that can grow around the B mining areas in Turkey, where the B concentration in soil reaches a lethal dose for plants (up to ∼8900mgkg(-1) (∼140mM). While its interesting survival capacity in extremely B containing soils, any molecular research has been conducted to understand its high tolerance mechanism yet. In the present study, we have performed a proteomic analysis of this plant to understand its high tolerance towards B-stress. Seedlings of G...
July 4, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28731488/contamination-risk-of-synovial-biopsy-cultures-in-total-hip-arthroplasty-a-prospective-review-of-100-cases
#3
Christophe Pattyn, Thomas De Bo, Joris Anthonissen, Philippe Willekens, Geert Claeys, Emmanuel A Audenaert
INTRODUCTION: Cultures of deep synovial biopsies remain an important tool in diagnosing periprosthetic joint infection, a devastating complication following total hip arthroplasty (THA). Recent reports of unexpected positive intraoperative cultures in aseptic revision arthroplasty, however, challenge the validity and interpretation of these cultures. The aim of this study was to evaluate the contamination risk of synovial biopsy cultures collected intraoperatively during primary THA of healthy subjects...
July 19, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/28731323/analyzing-liposomal-drug-delivery-systems-in-three-dimensional-cell-culture-models-using-maldi-imaging-mass-spectrometry
#4
Jessica K Lukowski, Eric M Weaver, Amanda B Hummon
Cancer chemotherapeutics often fail to reach all diseased cells. To help solve this problem, researchers are investigating novel drug delivery systems. Liposomes are an attractive option due to their low toxicity, high biocompatibility, and potential to carry a large amount of a drug to the tumor site, all while avoiding being eliminated from the body. This study evaluates the penetration of doxorubicin-encased liposomes into three-dimensional cell cultures, or spheroids. Liposomes composed of lipids containing head groups of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol were created by extrusion...
July 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28730308/tlc-surface-integrity-affects-the-detection-of-alkali-adduct-ions-in-tlc-maldi-analysis
#5
Yonghui Dong, Ruggero Ferrazza, Andrea Anesi, Graziano Guella, Pietro Franceschi
Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730086/peptidome-profiling-of-human-serum-of-uveal-melanoma-patients-based-on-magnetic-bead-fractionation-and-mass-spectrometry
#6
Xiang-Yu Shi, Qing Li, Wen-Bin Wei, Li-Ming Tao
AIM: To find new biomarkers for uveal melanoma (UM) by analyzing the serum peptidome profile. METHODS: Proteomic spectra in patients with UM before and after operation were analyzed and compared with those of healthy controls. Magnetic affinity beads were used to capture serum peptides and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer were used to compile serum peptide profiles. RESULTS: A panel of 49 peptides were differentially expressed between UM patients and controls, of which 33 peptides were of higher intensities in patient group and 16 peptides were of higher intensities in control group...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728930/specific-enrichment-of-glycoproteins-with-polymer-monolith-functionalized-with-glycocluster-grafted-%C3%AE-cyclodextrin
#7
Hai-Jiao Zheng, Jiu-Tong Ma, Wei Feng, Qiong Jia
The low abundance of glycoproteins in complex samples results in the prerequisite role of efficient and selective enrichment of them. In the present work, we designed a new kind of glycosylation poly(hydroxyethyl methacrylate-pentaerythritol triacrylate) monolith functionalized with glycocluster grafted β-cyclodextrin for the enrichment of glycoproteins. The introduced modifiers endowed the monolithic material with enhanced hydrophilicity and surface area, which benefitted to improve the enrichment selectivity and extraction efficiency for glycopeptides...
July 11, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28727449/the-use-of-maldi-ms-combined-with-differential-hydrogen-deuterium-exchange-for-semi-automated-protein-global-conformational-screening
#8
Gregory F Pirrone, Heather Wang, Nicole Canfield, Alexander S Chin, Timothy A Rhodes, Alexey A Makarov
Matrix-assisted laser desorption/ionization (MALDI) coupled with a time-of-flight (TOF) mass-spectrometry (MS) detector is acknowledged to be very useful for analysis of biological molecules. At the same time, hydrogen-deuterium exchange (HDX) is a well-known technique for studying protein higher-order structure. However, coupling MALDI with HDX may be challenging due to undesired back-exchange reactions during analysis. In this report, we survey an approach that utilizes MALDI coupled with automated sample preparation to compare global conformational changes of proteins under different solution conditions using differential HDX...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727443/plasmonic-thermal-decomposition-digestion-of-proteins-a-rapid-on-surface-protein-digestion-technique-for-mass-spectrometry-imaging
#9
Rong Zhou, Franco Basile
A method based on plasmon surface resonance absorption and heating was developed to perform a rapid on-surface protein thermal decomposition and digestion suitable for imaging MS and/or profiling. This photo-thermal process or plasmonic thermal decomposition/digestion (plasmonic-TDD) method incorporates a continuous wave (CW) laser excitation and gold nanoparticles (Au-NPs) to induce known thermal decomposition reactions that cleave peptides and proteins specifically at the C-terminus of aspartic acid and at the N-terminus of cysteine...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727417/oxygen-dependent-lipid-profiles-of-3d-cultured-human-chondrocytes-revealed-by-maldi-msi
#10
Brenda Bakker, Gert B Eijkel, Ron M A Heeren, Marcel Karperien, Janine N Post, Berta Cillero Pastor
Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supraphysiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (≤ 8%) promotes the chondrogenic phenotype. Exposure of cells to various oxygen levels alters their lipid metabolism, but detailed studies examining how hypoxia affects lipid metabolism in chondrocytes are lacking. To better understand the chondrocyte's behavior in response to oxygen we cultured 3D pellets of human primary chondrocytes in normoxia (20% oxygen) and hypoxia (2...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28726036/proteolytic-effects-of-gingipains-on-trefoil-factor-family-peptides
#11
Ponlatham Chaiyarit, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Barbara Potempa, Jan Potempa
OBJECTIVES: The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis. MATERIALS AND METHODS: Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease...
July 19, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28724559/identification-of-leishmania-by-maldi-tof-mass-spectrometry-using-a-free-web-based-application-and-a-dedicated-mass-spectral-library
#12
Laurence Lachaud, Anna Fernández-Arévalo, Anne Cécile Normand, Patrick Lami, Cécile Nabet, Jean Luc Donnadieu, Martine Piarroux, Farid Djenad, Carole Cassagne, Christophe Ravel, Silvia Tebar, Teresa Llovet, Denis Blanchet, Magalie Demar, Zoubir Harrat, Karim Aoun, Patrick Bastien, Carmen Muñoz, Montserrat Gállego, Renaud Piarroux
Human leishmaniasis are widely spread diseases with different clinical forms caused by about 20 species within the Leishmania genus. Leishmania species identification is relevant for therapeutic management and prognosis, especially for cutaneous and muco-cutaneous forms. Several methods are available to identify Leishmania species from culture, but they have not been standardized for the majority of the currently described species, with the exception of MultiLocus Enzyme Electrophoresis. Moreover, these techniques are expensive, time-consuming and not available in all laboratories...
July 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28724284/inter-alpha-inhibitor-h4-as-a-potential-biomarker-predicting-the-treatment-outcomes-in-patients-with-hepatocellular-carcinoma
#13
Eun-Jung Lee, Seung-Hyun Yang, Kyoung-Jin Kim, Hyejung Cha, Seo Jin Lee, Ji-Hye Kim, Junkyu Song, Kyung-Hee Chun, Jinsil Seong
Purpose: Early prediction of treatment outcomes represents an essential step towards increased treatment efficacy and survival in patients with hepatocellular carcinoma (HCC). In this study, we performed two-dimensional electrophoresis (2-DE) followed by protein profiling to identify biomarkers predictive of therapeutic outcomes in patients with HCC who received liver-directed therapy (LDTx) involving local radiotherapy (RT), and studied the underlying mechanisms of the identified proteins...
July 17, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28722797/highly-selective-enrichment-of-phosphopeptides-by-on-chip-indium-oxide-functionalized-magnetic-nanoparticles-coupled-with-maldi-tof-ms
#14
Dandan Jiang, Naizhong Song, Xiqian Li, Jiutong Ma, Qiong Jia
Selective and efficient preconcentration is indispensable for low concentration of phosphopeptides in phosphorylated protein-related samples prior to MS-based analysis. Herein, an on-chip system coupled magnetic SPE with MALDI-TOF MS was designed. A metal oxide affinity chromatography (MOAC) material, indium oxide, was coated on the surface of Fe3 O4 magnetic nanoparticles (MNPs) to prepare the adsorbent, spatially confined with an applied magnetic field. The adsorbent exhibited high selectivity for phosphopeptides in tryptic digests of the mixture of β-casein and BSA (1:1000) and the mixture of β-casein, BSA, and ovalbumin (1:100:100)...
July 19, 2017: Proteomics
https://www.readbyqxmd.com/read/28722283/comparative-analysis-of-storage-conditions-and-homogenization-methods-for-tick-and-flea-species-for-identification-by-maldi-tof-ms
#15
A Nebbak, B El Hamzaoui, J-M Berenger, I Bitam, D Raoult, L Almeras, P Parola
Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months...
July 19, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28721611/bone-targeting-parathyroid-hormone-conjugates-outperform-unmodified-pth-in-the-anabolic-treatment-of-osteoporosis-in-rats
#16
Yang Yang, Ali Aghazadeh-Habashi, Arash Panahifar, Yuchin Wu, Krishna H Bhandari, Michael R Doschak
Synthetic parathyroid hormone (PTH) is clinically indicated for the treatment of osteoporosis, through its anabolic effects on parathyroid hormone receptors (PTHRs), located on osteoblast cells. However, the bioavailability of PTH for bone cells is restricted by the short half-life of PTH and the widespread distribution of PTHRs in non-skeletal tissues. To impart affinity for mineralized bone surfaces, bisphosphonate (BP)-mediated PTH analogues were synthesized, characterized, and evaluated in vitro and in vivo...
August 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28718648/multi-imaging-of-cytokinin-and-abscisic-acid-on-the-roots-of-rice-oryza-sativa-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#17
Katsuhiro Shiono, Riho Hashizaki, Toyofumi Nakanishi, Tatsuko Sakai, Takushi Yamamoto, Koretsugu Ogata, Ken-Ichi Harada, Hajime Ohtani, Hajime Katano, Shu Taira
Plant hormones are important mobile endogenous signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Visual information on more than one plant hormone from the same sample would be especially powerful, because it could enable rapid, intuitive interpretation. However, multi-imaging techniques for plant hormones have been unavailable. Here, we report the first multi-imaging of two plant hormones, one of cytokinin (i.e. trans-zeatin) and abscisic acid (ABA) by MALDI-TOF-MS imaging...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28718376/riz1-and-histone-methylation-status-in-pituitary-adenomas
#18
Yake Xue, Ruokun Chen, Wei Du, Fengdong Yang, Xinting Wei
RIZ1 displays strong tumor-suppressive activities, which has a potential histone methyltransferase activity. The objective of the study was to evaluate the level and the methylation status of RIZ1 and analyze its association with clinicopathological features and the histone in the pituitary adenomas. We found that RIZ1-positive cases were 11/50 and H-Scores 22.75 ± 11.83 in invasive pituitary adenomas and 26/53 and 66.3 ± 21.7 in non-invasive pituitary adenomas (χ(2) = 8.182, p = 0.004). RIZ1 and C-myc showed the opposite trend in these cases...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28717220/imaging-mass-spectrometry-and-ms-ms-molecular-networking-reveals-chemical-interactions-among-cuticular-bacteria-and-pathogenic-fungi-associated-with-fungus-growing-ants
#19
Cristopher A Boya P, Hermógenes Fernández-Marín, Luis C Mejía, Carmenza Spadafora, Pieter C Dorrestein, Marcelino Gutiérrez
The fungus-growing ant-microbe symbiosis is an ideal system to study chemistry-based microbial interactions due to the wealth of microbial interactions described, and the lack of information on the molecules involved therein. In this study, we employed a combination of MALDI imaging mass spectrometry (MALDI-IMS) and MS/MS molecular networking to study chemistry-based microbial interactions in this system. MALDI IMS was used to visualize the distribution of antimicrobials at the inhibition zone between bacteria associated to the ant Acromyrmex echinatior and the fungal pathogen Escovopsis sp...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716730/interaction-of-small-g-protein-signaling-modulator-3-with-connexin-43-contributes-to-myocardial-infarction-in-rat-hearts
#20
Chang Youn Lee, Jung-Won Choi, Sunhye Shin, Jiyun Lee, Hyang-Hee Seo, Soyeon Lim, Seahyoung Lee, Hyun-Chul Joo, Sang Woo Kim, Ki-Chul Hwang
Connexin 43 (Cx43), a ubiquitous connexin expressed in the heart and skin, is associated with a variety of hereditary conditions. Therefore, the characterization of Cx43-interacting proteins and their dynamics is important to understand not only the molecular mechanisms underlying pathological malfunction of gap junction-mediated intercellular communication but also to identify novel and unanticipated biological functions of Cx43. In the present study, we observed potential targets of Cx43 to determine new molecular functions in cardio-protection...
July 14, 2017: Biochemical and Biophysical Research Communications
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