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Mercedes Marín, Adrián Ruiz, Cristina Iglesias, Lidia Quiroga, Emilia Cercenado, Pablo Martín-Rabadán, Emilio Bouza, Belén Rodríguez-Sánchez
OBJECTIVES: The identification of Nocardia isolates still represents a challenge for MALDI-TOF MS despite its acceptance for most bacterial and fungal isolates. In this study we evaluate the identification of Nocardia isolates using direct spotting and an updated database. METHODS: Overall, 82 Nocardia isolates belonging to 13 species were identified by DNA sequence analysis of the 16S rRNA and secA1 genes. Nine of these well-characterized isolates from 6 Nocardia species were used to create an in-house library...
June 19, 2018: Clinical Microbiology and Infection
Rashmi Kurli, Diptaraj Chaudhari, Aabeejjeet N Pansare, Mitesh Khairnar, Yogesh S Shouche, Praveen Rahi
A substantial majority of global population owns cellular phones independently to demographic factors like age, economic status, and educational attainment. In this study, we investigated the diversity of microorganisms associated with cellular phones of 27 individuals using cultivation-based methods. Cellular phones were sampled using cotton swabs and a total of 554 isolates representing different morphotypes were obtained on four growth media. Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry could generate protein profiles for 527 isolates and species-level identification was obtained for 415 isolates...
2018: Frontiers in Microbiology
Sarah Lepuschitz, Steliana Huhulescu, Patrick Hyden, Burkhard Springer, Thomas Rattei, Franz Allerberger, Robert L Mach, Werner Ruppitsch
The increasing emergence of multi-resistant bacteria in healthcare settings, in the community and in the environment represents a major health threat worldwide. In 2016, we started a pilot project to investigate antimicrobial resistance in surface water. Bacteria were enriched, cultivated on selective chromogenic media and species identification was carried out by MALDI-TOF analysis. From a river in southern Austria a methicillin resistant Staphylococcus aureus (MRSA) was isolated. Whole genome sequence analysis identified the isolate as ST8, spa type t008, SCCmecIV, PVL and ACME positive, which are main features of CA-MRSA USA300...
June 21, 2018: Scientific Reports
Seung Ji Kang, Su-Mi Choi, Jin-A Choi, Jin Un Choi, Tae-Hoon Oh, Seong Eun Kim, Uh Jin Kim, Eun Jeong Won, Hee-Chang Jang, Kyung-Hwa Park, Jong Hee Shin, Sun-Seog Kweon, Sook-In Jung
This study aimed to identify clinical or microbiological factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures. A total of 64 isolates from 51 patients identified as C. striatum by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing were assessed. More than two blood cultures were positive in 25 (48.1%) patients. Diabetes, solid tumor, and a history of previous exposure to antibiotics were more common in patients with multiple positive blood cultures...
2018: PloS One
V A Stepanov, A V Bocharova, K V Vagaitseva, A V Marusin, V V Markova, L I Minaicheva, I A Zhukova, N G Zhukova, V M Alifirova, O A Makeeva
AIM: To estimate the association of rs11218343 in the sortilin-related receptor 1 (SORL1) gene with cognitive performance in the elderly and with Alzheimer's disease (AD) in the Russian population. MATERIAL AND METHODS: A sample included 586 elderly people (mean age 70.9±5.7 years) without AD diagnosis and 100 patients with late-onset AD (mean age 72.1±7.8 years) from the Tomsk population. SORL1 rs11218343 was genotyped using PCR and MALDI-TOF mass spectrometry...
2018: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
Judy Park DeWitt, Cloyce L Stetson, Kleesy L Thomas, Bryan J Carroll
No abstract text is available yet for this article.
July 2018: Journal of Cutaneous Medicine and Surgery
Guangfeng Kan, Xiaofei Wang, Jie Jiang, Chengsheng Zhang, Minglei Chi, Yun Ju, Cuijuan Shi
Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea-ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control...
June 21, 2018: MicrobiologyOpen
Eiji Sugiyama, Mitsutoshi Setou
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a histological method used for various molecules including gangliosides. The method is based on mass spectrometry, and thus target molecules with small structural differences are directly and independently detected. Here we describe a general procedure and related key notes for analyzing major brain gangliosides by MALDI-IMS.
2018: Methods in Molecular Biology
Akemi Suzuki, Minoru Suzuki, Emi Ito, Takahiro Nitta, Jin-Ichi Inokuchi
This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM...
2018: Methods in Molecular Biology
Claudia Gebhard, Dagmar Glatz, Lucia Schwarzfischer, Julia Wimmer, Sebastian Stasik, Margit Nuetzel, Daniel Heudobler, Reinhard Andreesen, Gerhard Ehninger, Christian Thiede, Michael Rehli
Malignant transformation is frequently associated with disease-specific epigenetic alterations, but the underlying mechanisms and pathophysiological consequences remain poorly understood. Here, we used global comparative DNA methylation profiling at CG-rich regions of 27 acute myeloid leukemia (AML) samples to select a subset of aberrantly methylated CG-rich regions (~400 regions, ~15,000 CpGs) for quantitative DNA methylation profiling in a large cohort of AML patients (n = 196) using MALDI-TOF analysis of bisulfite-treated DNA...
June 20, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Y N Li, M Li, L Jiang, X H Luan, N Liang, Q N Xu, J S Zhang, M C Tang, Y N Bian, L Q Chen
OBJECTIVES: To evaluate the application of 43-plex SNP typing system in forensic science. METHODS: The typing of 43 SNP loci in 123 unrelated Han individuals from East China was detected by MALDI-TOF-MS. The application value of 43-plex SNP typing system was assessed according to the forensic parameters of population genetics. RESULTS: All the 43 SNP loci of 123 individuals showed no significant departure from Hardy-Weinberg equilibrium ( P >0...
April 2018: Fa Yi Xue za Zhi
Renata Urban-Chmiel, Andrzej Wernicki, Jacek Wawrzykowski, Andrzej Puchalski, Anna Nowaczek, Marta Dec, Diana Stęgierska, Mohammed Mijbas Mohammed Alomari
The aim of study was to isolate, characterize and analyse the protein profiles of Myoviridae-like bacteriophages obtained from M. haemolytica using MALDI TOF mass spectrometry. The material consisted of the M. haemolytica reference strain ATCC® BAA410, reference serotypes A1, A2, A5, A6, A7, A9, and A11, and wild-type isolates of serotype A1. Bacteriophage morphology was examined with a transmission electron microscope. The proteins were separated in SDS-PAGE and two-dimensional electrophoresis and characterized by MALDI-TOF...
June 19, 2018: AMB Express
Changqing Lu, Helei Jia, Aiguo Xu
Tumor necrosis factor superfamily member 4 (TNFSF4), also known as Ox40 ligand (Ox40l), plays an important role in atherosclerosis development. Several studies reported the association between the rs3850641 polymorphism of the TNFSF4 gene and the risk of myocardial infarction (MI). However, the results are inconsistent. In order to explore the relationship between the rs3850641 polymorphism of the TNFSF4 gene and MI, we conducted a case-control study including 454 cases and 512 controls in a Chinese Han population...
June 19, 2018: Bioscience Reports
Chih-Kai Chao, Narayanaganesh Balasubramanian, John M Gerdes, Charles M Thompson
In this study, the mechanisms of HuAChE and HuBChE inhibition by Me-P(O) (OPNP) (OR) [PNP = p-nitrophenyl; R = CH2 CH3 , CH2 CH2 F, OCH(CH3 )2 , OCH(CH3 ) (CH2 F)] representing surrogates and fluoro-surrogates of VX and sarin were studied by in vitro kinetics and mass spectrometry. The in vitro measures showed that the VX- and fluoro-VX surrogates were relatively strong inhibitors of HuAChE and HuBChE (ki  ∼ 105 -106  M-1 min-1 ) and underwent spontaneous and 2-PAM-mediated reactivation within 30 min...
June 16, 2018: Chemico-biological Interactions
Alireza Banazadeh, Wenjing Peng, Lucas Veillon, Yehia Mechref
Glycomics continues to be a highly dynamic and interesting research area due to the need to comprehensively understand the biological attributes of glycosylation in many important biological functions such as the immune response, cell development, cell differentiation/adhesion, and host-pathogen interactions. Although matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) has proven to be suitable for glycomic profiling studies, there is a need for improved sensitivity in the detection of native glycans, which ionize inefficiently...
June 18, 2018: Journal of the American Society for Mass Spectrometry
Irene Jiménez-Munguía, Mónica Calderón-Santiago, Antonio Rodríguez-Franco, Feliciano Priego-Capote, Manuel J Rodríguez-Ortega
We applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae to elucidate global changes in the bacterium in a condition similar to what can be found in the host during an infectious episode. We treated the reference strain TIGR4 with the iron chelator deferoxamine mesylate. DNA microarrays revealed changes in the expression of operons involved in multiple biological processes, with a prevalence of genes coding for ion binding proteins...
2018: PeerJ
Awatef Ouertani, Ines Chaabouni, Amor Mosbah, Justine Long, Mohamed Barakat, Pascal Mansuelle, Olfa Mghirbi, Afef Najjari, Hadda-Imene Ouzari, Ahmed S Masmoudi, Marc Maresca, Philippe Ortet, Didier Gigmes, Kamel Mabrouk, Ameur Cherif
Milk and dairy products harbor a wide variety of bacterial species that compete for both limited resources and space. Under these competitive conditions, bacteria develop specialized mechanisms to protect themselves during niche colonization and nutrient acquisition processes. The bacterial antagonism mechanisms include the production of antimicrobial agents or molecules that facilitate competitor dispersal. In the present work, a bacterial strain designated RC6 was isolated from Ricotta and identified as Bacillus cereus ...
2018: Frontiers in Microbiology
Thomas E Rams, Jacqueline D Sautter, Chinhua Y Hsiao, Arie J van Winkelhoff
The accuracy of a phenotypic scheme to recognize periodontal Prevotella intermedia/nigrescens group clinical isolates on primary isolation culture plates was assessed with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 84 fresh subgingival isolates from 23 chronic periodontitis patients were presumptively recognized on anaerobically-incubated enriched Brucella blood agar primary isolation plates as P. intermedia/nigrescens based on their dark-pigmented colony morphology, brick-red autofluorescence under long-wave ultraviolet light, and a negative fluorescence test for lactose production...
June 15, 2018: Anaerobe
Matthew W McCarthy, Aspasia Katragkou, Elias Iosifidis, Emmanuel Roilides, Thomas J Walsh
Species of Scedosporium and Fusarium are considered emerging opportunistic pathogens, causing invasive fungal diseases in humans that are known as scedosporiosis and fusariosis, respectively. These mold infections typically affect patients with immune impairment; however, cases have been reported in otherwise healthy individuals. Clinical manifestations vary considerably, ranging from isolated superficial infection to deep-seated invasive infection—affecting multiple organs—which is often lethal...
June 18, 2018: Journal of Fungi (Basel, Switzerland)
C Sartori, V Perreten, I Ivanovic, M C Härdi-Landerer, H U Graber
In Switzerland, sanitation programs of dairy herds infected with the contagious mastitis pathogen Staphylococcus aureus genotype B (GTB) have been established for several years. In recent years, Streptococcus uberis and non-aureus staphylococci have emerged as the bacteria most frequently isolated from bovine milk samples. The latter cause subclinical mastitis, and some species are more persistent or pathogenic than others. The present study aimed to investigate the developments in the intramammary colonization spectrum of 5 dairy herds undergoing a sanitation program for Staph...
June 13, 2018: Journal of Dairy Science
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