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https://www.readbyqxmd.com/read/29677683/application-of-solid-phase-microextraction-followed-by-liquid-chromatography-mass-spectrometry-in-the-determination-of-antibiotic-drugs-and-their-metabolites-in-human-whole-blood-and-tissue-samples
#1
Malgorzata Szultka-Mlynska, Pawel Pomastowski, Boguslaw Buszewski
A sensitive, rapid and specific analytical method using high performance liquid chromatography coupled with mass spectrometry (HPLC-QqQ-MS) was developed to determine selected antibiotic drugs and their metabolites (amoxicillin, cefotaxime, ciprofloxacin, clindamycin and metronidazole; amoxycilloic acid, 4-hydroxyphenyl glycyl amoxicillin, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, ciprofloxacin N-oxide, N-demethylclindamycin, clindamycin sulfoxide, and hydroxy metronidazole) in human whole blood and vascularized tissue after single oral administration...
April 12, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29675779/antibiotic-resistance-and-molecular-characteristics-of-staphylococcus-aureus-isolated-from-backyard-raised-pigs-and-pig-workers
#2
Asabe Halimat Momoh, Jacob K P Kwaga, Mohammed Bello, Anthony K B Sackey, Anders Rhod Larsen
Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes...
April 19, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29675742/specific-c%C3%AE-c-bond-cleavage-of-%C3%AE-carbon-centered-radical-peptides-produced-by-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#3
Keishiro Nagoshi, Mariko Yamakoshi, Kenya Sakamoto, Mitsuo Takayama
Radical-driven dissociation (RDD) of hydrogen-deficient peptide ions [M - H + H]·+ has been examined using matrix-assisted laser dissociation/ionization in-source decay mass spectrometry (MALDI-ISD MS) with the hydrogen-abstracting matrices 4-nitro-1-naphthol (4,1-NNL) and 5-nitrosalicylic acid (5-NSA). The preferential fragment ions observed in the ISD spectra include N-terminal [a] + ions and C-terminal [x]+ , [y + 2]+ , and [w]+ ions which imply that β-carbon (Cβ)-centered radical peptide ions [M - Hβ + H]·+ are predominantly produced in MALDI conditions...
April 19, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29675400/simultaneous-detection-of-key-bacterial-pathogens-related-to-pneumonia-and-meningitis-using-multiplex-pcr-coupled-with-mass-spectrometry
#4
Chi Zhang, Leshan Xiu, Yan Xiao, Zhengde Xie, Lili Ren, Junping Peng
Pneumonia and meningitis continue to present an enormous public health burden and pose a major threat to young children. Among the causative organisms of pneumonia and meningitis, bacteria are the most common causes of serious disease and deaths. It is challenging to accurately and rapidly identify these agents. To solve this problem, we developed and validated a 12-plex PCR coupled with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method (bacterial pathogen-mass spectrometry, BP-MS) that can be used to simultaneously screen for 11 key bacterial pathogens related to pneumonia and meningitis...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29674103/determination-of-the-cell-wall-polysaccharide-and-teichoic-acid-structures-from-lactococcus-lactis-il1403
#5
Evgeny Vinogradov, Irina Sadovskaya, Pascal Courtin, Saulius Kulakauskas, Thierry Grard, Jennifer Mahony, Douwe van Sinderen, Marie-Pierre Chapot-Chartier
In the lactic acid bacterium Lactococcus lactis, a cell wall polysaccharide (CWPS) is the bacterial receptor of the majority of infecting bacteriophages. The diversity of CWPS structures between strains explains, at least partially, the narrow host range of lactococcal phages. In the present work, we studied the polysaccharide components of the cell wall of the prototype L. lactis subsp. lactis strain IL1403. We identified a rhamnose-rich complex polysaccharide, carrying a glycerophosphate substitution, as the major component...
April 12, 2018: Carbohydrate Research
https://www.readbyqxmd.com/read/29674058/a-novel-protein-digestion-method-with-the-assistance-of-alternating-current-denaturation-for-high-efficient-protein-digestion-and-mass-spectrometry-analysis
#6
Xiaoxun Han, Yiming Xie, Qin Wu, Shuaibin Wu
Protein denaturation has always displayed a huge necessity for mass spectrometry (MS)-based protein identification methods in proteomics. In this research, a novel protein digestion method with the assistance of alternating current (AC) denaturation has been proposed and evaluated. In this method, merely, 200 mM ammonium bicarbonate buffer solution (pH, 8.2) was used to dissolve proteins and act as the electrolyte, and protein denaturation could be achieved in several seconds. For apo-transferrin, ovalbumin and bovine serum albumin that are resistant to digestion in their native states, confident amino acid sequence coverage by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis were obtained after 200 v AC denaturation...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29674052/glycan-affinity-magnetic-nanoplatforms-for-urinary-glycobiomarkers-discovery-in-bladder-cancer
#7
Rita Azevedo, Janine Soares, Cristiana Gaiteiro, Andreia Peixoto, Luís Lima, Dylan Ferreira, Marta Relvas-Santos, Elisabete Fernandes, Ana Tavares, Sofia Cotton, Ana Luísa Daniel-da-Silva, Lúcio Lara Santos, Rui Vitorino, Francisco Amado, José Alexandre Ferreira
Bladder Cancer (BC) presents one of the highest recurrence rates amongst solid tumours and constitutes the second deadliest disease of the genitourinary track. Non-invasive identification of patients facing disease recurrence and/or progression remains one of the most critical and challenging aspects in disease management. To contribute to this goal, we demonstrate the potential of glycan-affinity glycoproteomics nanoplatforms for urinary biomarkers discovery in bladder cancer. Briefly, magnetic nanoprobes (MNP) coated with three broad-spectrum lectins, namely Concanavalin A (ConA; MNP@ConA), Wheat Germ Agglutinin (WGA; MNP@WGA), and Sambucus nigra (SNA; MNP@SNA), were used to selectively capture glycoproteins from the urine of low-grade and high-grade non-muscle invasive as well as muscle-invasive BC patients...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29673864/pseudomonas-gallaeciensis-sp-nov-isolated-from-crude-oil-contaminated-intertidal-sand-samples-after-the-prestige-oil-spill
#8
Magdalena Mulet, David Sánchez, Ana C Rodríguez, Balbina Nogales, Rafael Bosch, Antonio Busquets, Margarita Gomila, Jorge Lalucat, Elena García-Valdés
Strains V113T , V92 and V120 have been isolated from sand samples taken at the Atlantic intertidal shore in Galicia, Spain, after the Prestige oil spill. A preliminary analysis of the 16S rRNA and the partial rpoD gene sequences indicated that these strains belonged to the Pseudomonas genus, but they were distinct from any known Pseudomonas species. They were extensively characterized by a polyphasic taxonomic approach and phylogenetic data that confirmed that these strains belonged to the Pseudomonas pertucinogena group...
April 11, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29673672/associations-between-a-single-nucleotide-polymorphism-of-stress-induced-phosphoprotein-1-and-endometriosis-adenomyosis
#9
Chia-Lung Tsai, Yun-Shien Lee, Angel Chao, Chih-Feng Yen, Hsin-Shih Wang, Tzu-Hao Wang
OBJECTIVE: We have recently reported that stress-induced phosphoprotein 1 (STIP1) is over-expressed in endometriosis/adenomyosis tissues. STIP1 may also be involved in immune regulation, thus we attempted to study the association between STIP1 single nucleotide polymorphisms (SNPs) and endometriosis/adenomyosis. MATERIALS AND METHODS: Five STIP1 SNPs (rs7941773, rs2845597, rs4980524, rs2282490, and rs2236647) were selected for genotyping with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in 286 patients with endometriosis/adenomyosis and 288 healthy postmenopausal controls...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29671790/chemical-synthesis-and-functional-analysis-of-varva-cyclotide
#10
Claudio A Álvarez, Paula A Santana, Omar Luna, Constanza Cárdenas, Fernando Albericio, María S Romero, Fanny Guzmán
Cyclotides are circular peptides found in various plant families. A cyclized backbone, together with multiple disulfide bonds, confers the peptides’ exceptional stability against protease digestion and thermal denaturation. In addition, the features of these antimicrobial molecules make them suitable for use in animal farming, such as aquaculture. Fmoc solid phase peptide synthesis on 2-chlorotrityl chlorine (CTC) resin using the “tea-bag” approach was conducted to generate the VarvA cyclotide identified previously from Viola arvensis ...
April 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29671439/maldi-tof-ms-characterization-of-n-linked-glycoprotein-derived-from-ginger-with-ace-inhibitory-activity
#11
Wenzhu Zhao, Yuejiao Chen, Siyu Xue, Zhipeng Yu, Hanjie Yu, Jingbo Liu, Jianrong Li, Feng Chen
Herein, the ability of ginger glycoproteins to inhibit the angiotensin-converting enzyme (ACE) is characterized. The activity is monitored via HPLC, and then the crude glycoproteins are enriched with lectin microarrays and magnetic microspheres. The N-linked glycans released from the enriched glycoproteins by PNGase F are identified by MALDI-TOF-MS. The results suggest that the crude ginger glycoproteins are active against ACE with an IC50 value of 0.83 ± 0.09 mg mL-1. The ginger glycoproteins are enriched by concanavalin A (Con A) and solanum tuberosum (Potato) lectin (STL), and the structures of the N-glycans released from the ginger glycoproteins include high-mannose type glycans, fucosylated-type glycans, and hybrid-type glycans, as analyzed by MALDI-TOF-MS...
April 19, 2018: Food & Function
https://www.readbyqxmd.com/read/29670585/maldi-spectra-database-for-rapid-discrimination-and-subtyping-of-mycobacterium-kansasii
#12
Jayaseelan Murugaiyan, Astrid Lewin, Elisabeth Kamal, Zofia Bakuła, Jakko van Ingen, Vit Ulmann, Miren J Unzaga Barañano, Joanna Humięcka, Aleksandra Safianowska, Uwe H Roesler, Tomasz Jagielski
Mycobacterium kansasii is an emerging non-tuberculous mycobacterial (NTM) pathogen capable of causing severe lung disease. Of the seven currently recognized M. kansasii genotypes (I-VII), genotypes I and II are most prevalent and have been associated with human disease, whereas the other five (III-VII) genotypes are predominantly of environmental origin and are believed to be non-pathogenic. Subtyping of M. kansasii serves as a valuable tool to guide clinicians in pursuing diagnosis and to initiate the proper timely treatment...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29669203/two-types-of-threonine-tagged-lipopeptides-synergize-in-host-colonization-by-pathogenic-burkholderia-species
#13
Tawatchai Thongkongkaew, Wei Ding, Evgeni Bratovanov, Emilia Oueis, Maria Garcia-Altares, Nestor Zaburannyi, Kirsten Harmrolfs, Youming Zhang, Kirstin Scherlach, Rolf Müller, Christian Hertweck
Bacterial infections of agriculturally important plants and mushrooms pose a major threat to human food sources worldwide. However, structures of chemical mediators required by the pathogen for host colonization and infection remain elusive in most cases. Here, we report two types of threonine-tagged lipopeptides conserved among mushroom and rice pathogenic Burkholderia species that facilitate bacterial infection of hosts. Genome mining, metabolic profiling of infected mushrooms and heterologous expression of orphan gene clusters allowed the discovery of these unprecedented metabolites in the mushroom pathogen Burkholderia gladioli (haereogladin, burriogladin), and the plant pathogen Burkholderia glumae (haereoglumin, and burrioglumin)...
April 18, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29668262/visualization-of-mycobacterial-biomarkers-and-tuberculosis-drugs-in-infected-tissue-by-maldi-ms-imaging
#14
Landry Blanc, Anne J Lenaerts, Véronique Dartois, Brendan Prideaux
MALDI mass spectrometry imaging (MALDI-MSI) is a technique capable of label-free identification and visualization of analytes in tissue sections. We have previously applied MALDI-MSI to study the spatial distribution of tuberculosis (TB) drugs in necrotic lung granulomas characteristic of pulmonary TB disease, revealing heterogeneous and often suboptimal drug distribution. To investigate the impact of differential drug distribution at the sites of infection, we sought to image mycobacterial biomarkers to co-register drugs and bacteria in lesion sections...
April 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29668258/characterization-of-fluorinated-polymers-by-atmospheric-solid-analysis-probe-high-resolution-mass-spectrometry-asap-hrms-combined-with-kendrick-mass-defect-analysis
#15
Gabriel Gaiffe, Richard B Cole, Sabrina Lacpatia, Maxime Cyril Bridoux
Fluorinated polymers are a diverse and important class of polymers with unique applications. However, characterization of fluorinated polymers by conventional mass spectrometric methods is challenging because: i) the high fluorine content makes them insoluble or only sparingly soluble in most common solvents; and ii) commonly used matrices employed for MALDI do not desorb/ionize them efficiently. In this work, Atmospheric Solid Analysis Probe (ASAP) high resolution OrbitrapTM mass spectrometry (HRMS) was used as a new tool for the molecular characterization of various fluorinated polymers including polyvinylidene fluoride (PVDF), and fluorinated copolymers containing PVDF and chlorotrifluoroethylene (KEL-F 800), or PVDF and hexafluoropropylene (Viton A, Tecnoflon)...
April 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29667236/recent-advances-in-matrix-assisted-laser-desorption-ionisation-mass-spectrometry-imaging-maldi-msi-for-in-situ-analysis-of-endogenous-molecules-in-plants
#16
Liang Qin, Yawen Zhang, Yaqin Liu, Huixin He, Manman Han, Yanyan Li, Maomao Zeng, Xiaodong Wang
INTRODUCTION: Mass spectrometry imaging (MSI) as a label-free and powerful imaging technique enables in situ evaluation of a tissue metabolome and/or proteome, becoming increasingly popular in the detection of plant endogenous molecules. OBJECTIVE: The characterization of structure and spatial information of endogenous molecules in plants are both very important aspects to better understand the physiological mechanism of plant organism. METHODS: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a commonly-used tissue imaging technique, which requires matrix to assist in situ detection of a variety of molecules on the surface of a tissue section...
April 17, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29666623/autoimmunity-to-tropomyosin-specific-peptides-induced-by-mycobacterium-leprae-in-leprosy-patients-identification-of-mimicking-proteins
#17
Itu Singh, Asha Ram Yadav, Keshar Kunja Mohanty, Kiran Katoch, Prashant Sharma, Vinay Kumar Pathak, Deepa Bisht, Umesh D Gupta, Utpal Sengupta
Background: It has been shown earlier that there is a rise in the levels of autoantibodies and T cell response to cytoskeletal proteins in leprosy. Our group recently demonstrated a rise in both T and B cell responses to keratin and myelin basic protein in all types of leprosy patients and their associations in type 1 reaction (T1R) group of leprosy. Objectives: In this study, we investigated the association of levels of autoantibodies and lymphoproliferation against myosin in leprosy patients across the spectrum and tried to find out the mimicking proteins or epitopes between host protein and protein/s of Mycobacterium leprae ...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29665737/increased-synthesis-of-a-new-oleanane-type-saponin-in-hairy-roots-of-marigold-calendula-officinalis-after-treatment-with-jasmonic-acid
#18
Michał Markowski, Marek Długosz, Anna Szakiel, Mathieu Durli, Sophie Poinsignon, Sabine Bouguet-Bonnet, Lionel Vernex-Loset, Gabriel Krier, Max Henry
Native plant of marigold (Calendula officinalis L.) synthesizes oleanolic acid saponins classified as glucosides or glucuronides according to the first residue in sugar chain bound to C-3 hydroxyl group. Hairy root culture, obtained by transformation with Agrobacterium rhizogenes strain 15834, exhibit a potent ability of synthesis of oleanolic acid glycosides. The HPLC profile of saponin fraction obtained from C. officinalis hairy roots treated with plant stress hormone, jasmonic acid, showed the 10-times increase of the content of one particular compound, determined by NMR and MALDI TOF as a new bisdesmoside saponin, 3-O-β-d-glucuronopyranosyl-28-O-β-d-galactopyranosyl-oleanolic acid...
April 18, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29663868/imaging-mass-spectrometry-of-endogenous-polypeptides-and-secondary-metabolites-from-galls-induced-by-root-knot-nematodes-in-tomato-roots
#19
Eder Alves Barbosa, Mauro Ferreira Bonfim Junior, Carlos Bloch, Thales Lima Rocha, Gilbert Engler, Janice de Almeida Engler
Nematodes are devastating pests that infect most cultivated plant species and cause considerable agricultural losses worldwide. The understanding of metabolic adjustments induced during plant-nematode interaction is crucial to generate resistant plants or to select more efficient molecules to fight against this pest. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used herein for in situ detection and mapping endogenous polypeptides and secondary metabolites from nematode-induced gall tissue...
April 17, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29663255/structural-characterization-and-disulfide-assignment-of-spider-peptide-ph%C3%AE-1%C3%AE-by-mass-spectrometry
#20
Kelly L Wormwood, Armand Gatien Ngounou Wetie, Marcus Vinicius Gomez, Yue Ju, Paul Kowalski, Marius Mihasan, Costel C Darie
Native Phα1β is a peptide purified from the venom of the armed spider Phoneutria nigriventer that has been shown to have an extensive analgesic effect with fewer side effects than ω-conotoxin MVIIA. Recombinant Phα1β mimics the effects of the native Phα1β. Because of this, it has been suggested that Phα1β may have potential to be used as a therapeutic for controlling persistent pathological pain. The amino acid sequence of Phα1β is known; however, the exact structure and disulfide arrangement has yet to be determined...
April 16, 2018: Journal of the American Society for Mass Spectrometry
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