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https://www.readbyqxmd.com/read/28646746/variable-spontaneous-mutation-rate-in-clinical-strains-of-multidrug-resistant-acinetobacter-baumannii-and-differentially-expressed-proteins-in-a-hypermutator-strain
#1
Morteza Karami-Zarandi, Masoumeh Douraghi, Behrouz Vaziri, Habibeh Adibhesami, Mohammad Rahbar, Mehdi Yaseri
BACKGROUND: The emergence of multidrug resistant Acinetobacter baumannii within hospitals poses a significant threat to patients. The inherent rate of mutation of these strains has not been described nor has the mechanism by which drug resistance arises. METHODS: Here, we determined the spontaneous mutation rates in 93 clinical strains of A. baumannii using fluctuation analysis. To rule out the clonal relatedness of hypermutator strains, pulsed-field gel electrophoresis (PFGE) was conducted...
June 8, 2017: Mutation Research
https://www.readbyqxmd.com/read/28646681/proteomic-analysis-of-developing-wheat-grains-infected-by-powdery-mildew-blumeria-graminis-f-sp-tritici
#2
Jie Li, Xi-Wen Yang, Yong-Chun Li, Ji-Shan Niu, De-Xian He
Blumeria graminis f.sp. tritici (Bgt) infection greatly interferes with the normal source-sink relationships and always causes tremendous loss of yield and quality in wheat. To better understand the impact of this pathogen on grain development, proteome characterization during grain development in susceptible wheat cultivar Xinong 979 infected by powdery mildew was investigated by 2-DE and tandem MALDI-TOF/TOF-MS. Identification of 111 differentially expressed protein spots representing 85 unique proteins and six expression patterns showed a chronological description of wheat grain formation...
June 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28645272/prosthetic-joint-infection-caused-by-granulicatella-adiacens-a-case-series-and-review-of-literature
#3
Fanny Quénard, Piseth Seng, Jean-Christophe Lagier, Florence Fenollar, Andreas Stein
BACKGROUND: Bone and joint infection involving Granulicatella adiacens is rare, and mainly involved in cases of bacteremia and infectious endocarditis. Here we report three cases of prosthetic joint infection involving G. adiacens that were successfully treated with surgery and prolonged antimicrobial treatment. We also review the two cases of prosthetic joint infection involving G. adiacens that are reported in the literature. CASE PRESENTATION: Not all five cases of prosthetic joint infection caused by G...
June 23, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28643898/identification-of-cross-reactive-b-cell-epitopes-between-bos-d-9-0101-bos-taurus-and-gly-m-5-0101-glycine-max-by-epitope-mapping-maldi-tof-ms
#4
Ángela María Candreva, Mario Ferrer-Navarro, Silvia Bronsoms, Alejandra Quiroga, Renata Curciarello, Ana Cauerhff, Silvana Petruccelli, Guillermo Horacio Docena, Sebastián Alejandro Trejo
Exposure to cow's milk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion of milk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described between soy and milk proteins . We have previously identified several cross-reactive allergens between milk and soy that may explain this intolerance. The purpose of the present work was to identify epitopes in the purified αS1-casein and the recombinant soy allergen Gly m 5...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28643493/identification-of-viridans-streptococci-with-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-an-in-house-method-and-a-commercially-available-system
#5
Catalina Suzana Stingu, Klaus Eschrich, Juliane Thiel, Toralf Borgmann, Reiner Schaumann, Arne C Rodloff
Two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based methods were compared for their ability to identify viridans streptococci. One approach employed a reference database and software developed in-house. All inhouse measurements were performed using an Autoflex II Instrument (Bruker Daltonics GmbH, Germany). The other system, a VITEK-MS (BioMérieux, France) was operated on the commercially available V2.0 Knowledge Base for Clinical Use database. Clinical isolates of viridans streptococci (n=184) were examined...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28643238/characterization-of-the-complex-involved-in-regulating-v-atpase-activity-of-the-vacuolar-and-endosomal-membrane
#6
REVIEW
Zhenyu Zhang, Xiaojiao Wang, Tangjie Gao, Chunyin Gu, Fubao Sun, Lin Yu, Jinguang Hu
Regulator of the H(+)-ATPase of the vacuolar and endosomal membranes (RAVE) is essential for the reversible assembly of H(+)-ATPase. RAVE primarily consists of three subunits: Rav1p, Rav2p and Skp1p. To characterize these subunits, in this study, four strains derived from Saccharomyces cerevisiae BY4742 were constructed with a FLAG tag on the Rav1p and Rav2p subunits. Then, the corresponding RAVE containing complex was isolated by affinity purification. Western blot and MALDI-TOF mass spectrometry analyses showed that the RAVE complex contains not only the known V1-ATPase subunits (Vma1p and Vma2p) but also a newly found Leu1p that interacts with the RAVE subunit...
June 22, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/28643153/a-3e8-scfv-cys-ir800-conjugate-targeting-tag-72-in-an-orthotopic-colorectal-cancer-model
#7
Li Gong, Haiming Ding, Nicholas E Long, Brandon J Sullivan, Edward W Martin, Thomas J Magliery, Michael F Tweedle
PURPOSE: Optical surgical navigation (OSN) will be a potent tool to help surgeons more accurately and efficiently remove tumors. The purpose of this study was to evaluate a novel humanized 3E8 antibody (3E8 MAb) fragment site-specifically conjugated with IR800, 3E8.scFv.Cys-IR800, as a potential OSN agent to target colorectal adenocarcinoma. PROCEDURES: An engineered single-chain variable fragment of 3E8 MAb (targeted to TAG-72), appending a C-terminal cysteine residue (3E8...
June 22, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28641919/prevalence-of-candida-africana-and-candida-dubliniensis-in-vulvovaginal-candidiasis-first-turkish-candida-africana-isolates-from-vulvovaginal-candidiasis
#8
G Hazirolan, H U Altun, R Gumral, N C Gursoy, B Otlu, B Sancak
INTRODUCTION: Candida africana and C. dubliniensis are closely related species of C. albicans. Current phenotypic methods are not suitable to accurately distinguish all the species belonging to the C. albicans complex. Several molecular-based methods have recently been designed for discriminating among closely related Candida species. The aim of this study was to establish the prevalence of C. dubliniensis and C. africana in vulvovaginal samples with phenotypic and genotypic methods...
June 19, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28640320/molecular-signature-of-coronary-stent-thrombosis-oxidative-stress-and-innate-immunity-cells
#9
Judit Cubedo, Ana Blasco, Teresa Padro, Ilaria Ramaiola, Oriol Juan-Babot, Javier Goicolea, José Antonio Fernández-Díaz, Juan Francisco Oteo, Lina Badimon
The clinical impact of in-stent thrombosis is high because it is associated with high mortality and 20 % of the patients suffer a recurrent event within the two following years. The aim of this study was to characterise the morphologic and proteomic profile of in-stent thrombi (IST) in comparison to thrombi developed on native coronary arteries (CT) to identify a differential molecular signature. The study included 45 patients with ST-elevation-myocardial-infarction (STEMI) treated by primary-percutaneous-intervention and thrombus aspiration: 21 had IST and 24 had CT...
June 22, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28639631/an-update-on-maldi-mass-spectrometry-based-technology-for-the-analysis-of-fingermarks-stepping-into-operational-deployment
#10
REVIEW
S Francese, R Bradshaw, N Denison
Since 2009, when Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI) was firstly reported for the molecular mapping of latent fingermarks, the range of information and operational capabilities have steadily increased. Pioneering work from our Fingermark Research Group exploited different modalities, including Profiling (MALDI MSP), tandem mass spectrometry (MS/MS) and Ion Mobility MS/MS; a number of methodologies were also developed to conquer a main challenge, namely profiling the suspect and their actions prior to or whilst committing the crime...
June 22, 2017: Analyst
https://www.readbyqxmd.com/read/28638578/a-rare-case-of-aerococcus-urinae-infective-endocarditis
#11
Harsha Tathireddy, Sahitya Settypalli, John J Farrell
Introduction:Aerococcus urinae is a rare cause of infective endocarditis. Aerococcus is a gram positive cocci that is easily misidentified as Staphylococci or Streptococci. The true incidence rate of this pathogen is likely underestimated. Recent advances in laboratory diagnostic methods with matrix-associated laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) have lead to increased recognition of this pathogen in the clinical microbiology lab, and awareness as a cause of infective endocarditis in the infectious disease community...
March 2017: Journal of Community Hospital Internal Medicine Perspectives
https://www.readbyqxmd.com/read/28637912/parallel-evaluation-of-the-maldi-sepsityper-and-verigene-bc-gn-assays-for-rapid-identification-of-gram-negative-bacilli-from-positive-blood-cultures
#12
Miguel A Arroyo, Gerald A Denys
Rapid identification of microorganisms from positive blood cultures has improved clinical management and antimicrobial stewardship. The advent of the matrix-assisted laser desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) has reduced the time to identification of cultured isolates and is now often the definitive method used in the clinical microbiology laboratory. The commercial in vitro diagnostic MALDI Sepsityper (Sepsityper) kit has the potential for standardization and clinical routine use for the rapid identification of a broad range of bacteria from positive blood cultures...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28637909/rapid-differentiation-of-haemophilus-influenzae-and-haemophilus-haemolyticus-using-maldi-tof-mass-spectrometry-with-clinprotools-mass-spectra-analysis
#13
Jonathan H K Chen, Vincent C C Cheng, Chun-Pong Wong, Sally C Y Wong, Wing-Cheong Yam, Kwok-Yung Yuen
Haemophilus influenzae is associated with severe invasive disease, while Haemophilus haemolyticus is considered part of the commensal flora in the human respiratory tract. Although the addition of a custom mass spectra library into the matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) system could improve identification of these two species, the establishment of such a custom database is technically complicated and requires a large amount of resources, which most clinical laboratories cannot afford...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28637907/online-identification-of-fungi-using-maldi-tof-mass-spectrometry-validation-of-a-new-web-application
#14
A C Normand, P Becker, F Gabriel, C Cassagne, I Accoceberry, M Gari-Toussaint, L Hasseine, D De Geyter, D Pierard, I Surmont, F Djenad, J L Donnadieu, M Piarroux, S Ranque, M Hendrickx, R Piarroux
MALDI-TOF mass spectrometry has emerged as a reliable technique to identify molds involved in human diseases, including dermatophytes, provided that exhaustive reference databases are available. This study assessed an online identification application based on original algorithms and an extensive in-house reference database comprising 11,851 spectra (938 fungal species and 246 fungal genera).Validation criteria were established using an initial panel of 422 molds, including dermatophytes, previously identified via DNA sequencing (126 species)...
June 21, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28636332/new-insights-into-the-wavelength-dependence-of-maldi-mass-spectrometry
#15
Marcel Niehaus, Andreas Schnapp, Annika Koch, Jens Soltwisch, Klaus Dreisewerd
The interplay between the wavelength of the laser and the absorption profile of the matrix constitutes a crucial factor in matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Numerous studies have shown that typically best analytical results are obtained if the laser wavelength matches the UV absorption band of the matrix in the solid state well. However, many powerful matrices exhibit peak absorptions which differ notably from the standard MALDI laser wavelengths of 337, 349, and 355 nm, respectively...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28636329/metallic-element-chelated-tag-labeling-mectl-for-quantitation-of-n-glycans-in-maldi-ms
#16
Lijun Yang, Ye Peng, Jing Jiao, Tao Tao, Jun Yao, Ying Zhang, Haojie Lu
N-glycosylation plays an important role in chief biological and pathological processes. Quantifying the N-glycan is important since glycan alterations are related to many diseases. In this study, we developed a novel N-glycan quantitation approach using metallic element chelated tag labeling through reductive amination (MeCTL). The MeCTL strategy is of high labeling efficiency and accurate in quantitation with high reproducibility (CV<17.03%) and good linearity (R2>0.99) within two orders of magnitude of dynamic range...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28636327/maldi-ms-guided-liquid-microjunction-extraction-for-ce-esi-ms-analysis-of-single-pancreatic-islet-cells
#17
Troy J Comi, Monika A Makurath, Marina C Philip, Stanislav S Rubakhin, Jonathan V Sweedler
The ability to characterize chemical heterogeneity in biological structures is essential to understanding cellular level function in both healthy and diseased states, but these variations remain difficult to assess using a single analytical technique. While mass spectrometry (MS) provides sufficient sensitivity to measure many analytes from volume-limited samples, each type of mass spectrometric analysis uncovers only a portion of the complete chemical profile of a single cell. Matrix-assisted laser desorp-tion/ionization (MALDI) MS and capillary electrophoresis electrospray ionization (CE-ESI)-MS are complementary analytical platforms frequently utilized for single cell analysis...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28635997/a-spin-column-free-approach-to-sodium-hydroxide-based-glycan-permethylation
#18
Yueming Hu, Chad R Borges
Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS...
June 21, 2017: Analyst
https://www.readbyqxmd.com/read/28634118/identification-of-chickpea-seed-proteins-resistant-to-simulated-in-vitro-human-digestion
#19
I C Ribeiro, C C Leclercq, N Simões, A Toureiro, I Duarte, J B Freire, M M Chaves, J Renaut, C Pinheiro
Proteins and peptides able to resist gastrointestinal digestion and reach the intestinal mucosa have the potential to influence human health. Chickpea (Cicer arietinum L.) seed proteins are able to resist cooking (86.9% total protein) and/or in vitro simulated human digestion (15.9% total protein resists soaking, cooking and digestion with pepsin and pancreatin). To identify and characterize proteins resisting digestion we made use of different MS methodologies. The efficiency of several proteases (trypsin, AspN, chymotrypsin and LysC) was tested, and two technologies were employed (MALDI-TOF-TOF and LC-nESI-MS/MS)...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28631328/phenotypic-characterisation-of-coagulase-negative-staphylococci-isolated-from-blood-cultures-in-newborn-infants-with-a-special-focus-on-staphylococcus-capitis
#20
Gustaf Ehlersson, Bengt Hellmark, Olov Svartström, Bianca Stenmark, Bo Söderquist
AIM: This Swedish study determined which species of coagulase-negative staphylococci (CoNS) were found in neonatal blood cultures and whether they included Staphylococcus capitis clones with decreased susceptibility to vancomycin. METHODS: CoNS isolates (n=332) from neonatal blood cultures collected at Örebro University Hospital during 1987-2014 were identified to species level with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)...
June 20, 2017: Acta Paediatrica
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