keyword
MENU ▼
Read by QxMD icon Read
search

MALDI

keyword
https://www.readbyqxmd.com/read/28812125/recombinant-expression-and-biochemical-characterization-of-mycobacterium-tuberculosis-3fe-4s-ferredoxin-rv1786
#1
Yun Lu, Feng Qiao, Yue Li, Xiao-Hong Sang, Cong-Ran Li, Jian-Dong Jiang, Xin-Yi Yang, Xue-Fu You
Ferredoxins are iron-sulfur protein that mediate electron transfer in cytochrome P450 mono-oxygenase (CYP)-related catalytic reactions in a wide variety of organisms. Rv1786 is a putative ferredoxin, encoded by a gene located downstream of the gene encoding CYP143A1 in the Mycobacterium tuberculosis genome. However, the structure and function of Rv1786 have remained unclear. Here, the recombinant Mtb Rv1786 was expressed, purified as a His-tagged form and characterized with [3Fe-4S] clusters as its cofactors using a series of measurements including SDS-PAGE, western blot, UV/Visible, MALDI-TOF/TOF-MS, and electron paramagnetic resonance spectroscopic analysis...
August 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28811116/identification-of-nocardia-streptomyces-and-tsukamurella-using-maldi-tof-ms-with-the-bruker-biotyper
#2
Melanie L Yarbrough, William Lainhart, Carey-Ann D Burnham
Nocardia species are the most commonly isolated aerobic actinomycetes from human clinical specimens. Our objective was to assess the identification of clinically relevant actinomycetes using the Bruker Biotyper MALDI-TOF system, including comparison of extraction methods, Biotyper library versions, score cutoffs, and media. Banked Streptomyces (n=10), Tsukamurella (n=2), and Nocardia isolates (n=60) were cultured and extracted using three methods: mycobacterial extraction, ethanol formic acid extraction, or direct on-target extraction...
July 1, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28811086/proteomic-profiling-of-the-antifungal-drug-response-of-aspergillus-fumigatus-to-voriconazole
#3
Nansalmaa Amarsaikhan, Daniela Albrecht-Eckardt, Christoph Sasse, Gerhard H Braus, Zumrut B Ogel, Olaf Kniemeyer
Antifungal resistance is an emerging problem and one of the reasons for treatment failure of invasive aspergillosis (IA). Voriconazole has become a standard therapeutic for the treatment of this often fatal infection. We studied the differentially expressed proteins as a response of Aspergillus fumigatus to voriconazole by employing the two-dimensional difference gel electrophoresis (DIGE) technique. Due to addition of drug, a total of 135 differentially synthesized proteins were identified by MALDI-TOF/TOF-mass spectrometry...
August 5, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28809925/salivary-peptidome-profiling-analysis-for-occurrence-of-new-carious-lesions-in-patients-with-severe-early-childhood-caries
#4
Chang Tian, Xiangyu Sun, Xiaochen Liu, Xin Huang, Feng Chen, Shuguo Zheng
This study aimed to identify differences of peptide profiles in stimulated whole saliva among children with and without occurrence of new carious lesions, and to provide a simple way for early diagnosis and prevention of the relapse of severe early childhood caries (s-ECC). Overall, 26 children aged 3-4 years were selected out from all the children in the kindergarten to be involved in the present study, among them 13 were diagnosed as s-ECC and underwent dental treatment, whilst the other 13 were matched by age and sex as control...
2017: PloS One
https://www.readbyqxmd.com/read/28807573/axially-substituted-silicon-iv-phthalocyanine-and-its-quaternized-derivative-as-photosensitizers-towards-tumor-cells-and-bacterial-pathogens
#5
İpek Ömeroğlu, Esra Nur Kaya, Meltem Göksel, Vesselin Kussovski, Vanya Mantareva, Mahmut Durmuş
Axially di-(alpha,alpha-diphenyl-4-pyridylmethoxy) silicon(IV) phthalocyanine (3) and its quaternized derivative (3Q) were synthesized and tested as photosensitizers against tumor and bacterial cells. These new phthalocyanines were characterized by elemental analysis, and different spectroscopic methods such as FT-IR, UV-Vis, MALDI-TOF and (1)H NMR. The photophysical properties such as absorption and fluorescence, and the photochemical properties such as singlet oxygen generation of both phthalocyanines were investigated in solutions...
August 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28807561/mass-spectrometry-analysis-of-intact-francisella-bacteria-identifies-lipid-a-structure-remodeling-in-response-to-acidic-ph-stress
#6
Camille B Robert, Michael Thomson, Alain Vercellone, Francesca Gardner, Robert K Ernst, Gérald Larrouy-Maumus, Jérôme Nigou
Structural modification of lipid A, the lipid anchor of LPS, is one of the strategies used by Gram-negative bacteria to evade host innate immunity. Francisella tularensis is a human pathogen that infects and replicates within phagocytic cells. It produces an atypical lipid A, whose structure precludes an efficient recognition by both innate immune players, TLR4 and cationic antimicrobial peptides. Interestingly, a recent report indicates that the lipid A of Francisella (LVS vaccinal strain) undergoes polar modifications when bacteria are grown in human macrophages as compared to in broth...
August 11, 2017: Biochimie
https://www.readbyqxmd.com/read/28807542/rapid-and-simple-analysis-of-disease-associated-biomarkers-of-taiwanese-patients-with-schizophrenia-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#7
Tiao-Lai Huang, Li-Hua Lo, Jentaie Shiea, Hung Su
BACKGROUND: Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS) is an extremely sensitive analytical tool for characterizing biological compounds in bio samples. In this study, we applied MALDI-TOF MS to assess potential protein biomarkers in the peripheral blood mononuclear cells (PBMCs) of patients with schizophrenia in the acute phase, recovery phase and healthy controls in Taiwan. METHODS: We recruited 40 participants, including 20 pairs of patients diagnosed with schizophrenia in the acute phase, after four-week treatment with drug in the recovery phase, and 20 healthy controls...
August 11, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28806859/analysis-of-low-molecular-weight-compounds-using-maldi-and-ldi-tof-ms-direct-detection-of-active-pharmaceutical-ingredients-in-different-formulations
#8
João L Bronzel, Cintia D F Milagre, Humberto M S Milagre
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) is a high throughput, easy to use analytical technique. The simple sample preparation of this technique and its tolerance to the presence of contaminants are among its advantages. In contrast, depending on the matrix used, MALDI can ionize and generates ions in the low m/z range that complicate the interpretation of the spectra of low molecular weight compounds. To address this issue, one can envisage the use of tunable ionic matrices that can reduce the low m/z interferents...
August 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28806645/evaluation-of-the-binding-of-four-anti-tumor-casiope%C3%A3-nas%C3%A2-to-human-serum-albumin
#9
Isabel Correia, Sladjana Borovic, Isabel Cavaco, Cristina P Matos, Somnath Roy, Hugo M Santos, Luz Fernandes, José L Capelo, Lena Ruiz-Azuara, João Costa Pessoa
The metal complexes designated by Casiopeínas® are mixed-ligand Cu(II)-compounds some of them having promising antineoplastic properties. We report studies of binding of Cu(glycinato)(4,7-dimethyl-1,10-phenanthroline) (Cas-II-Gly (1)), Cu(acetylacetonato)(4,7-dimethyl-1,10-phenanthroline) (Cas-III-Ea (2)), Cu(glycinato)(4,4'-dimethyl-2,2'-bipyridine) (Cas-IV-Gly (3)) and Cu(acetylacetonato)(4,4'-dimethyl-2,2'-bipyridine) (Cas-III-ia (4)) to human serum albumin (HSA) by circular dichroism (CD), Electron paramagnetic resonance (EPR) and fluorescence spectroscopy...
July 29, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28805727/leaf-physiological-and-proteomic-analysis-to-elucidate-silicon-induced-adaptive-response-under-salt-stress-in-rosa-hybrida-rock-fire
#10
Prabhakaran Soundararajan, Abinaya Manivannan, Chung Ho Ko, Sowbiya Muneer, Byoung Ryong Jeong
Beneficial effects of silicon (Si) on growth and development have been witnessed in several plants. Nevertheless, studies on roses are merely reported. Therefore, the present investigation was carried out to illustrate the impact of Si on photosynthesis, antioxidant defense and leaf proteome of rose under salinity stress. In vitro-grown, acclimatized Rosa hybrida 'Rock Fire' were hydroponically treated with four treatments, such as control, Si (1.8 mM), NaCl (50 mM), and Si+NaCl. After 15 days, the consequences of salinity stress and the response of Si addition were analyzed...
August 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28803462/structural-characterization-of-methylenedianiline-regioisomers-by-ion-mobility-and-mass-spectrometry-iii-maldi-spectra-of-2-ring-isomers
#11
Sarah M Stow, Tiffany M Crescentini, Jay G Forsythe, Jody C May, John A McLean, David M Hercules
Characterization of methylenedianiline (MDA) 2-ring isomers (2,2'-, 2,4'- and 4,4'-MDA) is reported using matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS), a common technique used for characterizing synthetic polymers. MDA is a precursor to methylene diphenyl diisocyanate (MDI) a hard block component in polyurethane (PUR) synthesis. This manuscript focuses on comparing MALDI results to those of our previous electrospray ionization-mass spectrometry (ESI-MS) studies. In ESI, 2-ring MDA isomers formed single unique [M+H]+ (199 Da) parent ions, whereas in MALDI each isomer shows significant formation of three precursor ions: [M-H]+ = 197 Da, [M...
August 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28803337/production-of-rhamnolipids-by-semi-solid-state-fermentation-with-pseudomonas-aeruginosa-rg18-for-heavy-metal-desorption
#12
Jianrong Wu, Jingbo Zhang, Panpan Wang, Li Zhu, Minjie Gao, Zhiyong Zheng, Xiaobei Zhan
Foaming problem and cost of substrate limit the commercial application of rhamnolipids, a potential biosurfactant produced by Pseudomonas aeruginosa. We explored the production of rhamnolipids by a semi-solid-state (SSS) fermentation strategy with glycerol as carbon source and rapeseed/wheat bran as matrix, along with the capacity of the produced rhamnolipids to solubilize lead and cadmium in aqueous solution. Structural analysis by MALDI-TOF MS indicated the increased proportion of mono-rhamnolipids from SSS fermentation...
August 12, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28802703/identification-and-characterization-of-clostridium-botulinum-group-iii-field-strains-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#13
Luca Bano, Ilenia Drigo, Elena Tonon, Simone Pascoletti, Cinzia Puiatti, Fabrizio Anniballi, Bruna Auricchio, Florigio Lista, Cesare Montecucco, Fabrizio Agnoletti
Animal botulism is primarily due to botulinum neurotoxin (BoNT) types C, D or their chimeric variants C/D or D/C, produced by Clostridium botulinum group III, which appears to include the genetically indistinguishable Clostridium haemolyticum and Clostridium novyi. In the present study, we used matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI TOF MS) to identify and characterize 81 BoNT-producing Clostridia isolated in 47 episodes of animal botulism. The instrument's default database, containing no entries for Clostridium botulinum, permitted reliable identification of 26 strains at the genus level...
August 9, 2017: Anaerobe
https://www.readbyqxmd.com/read/28802585/genomics-of-the-new-species-kingella-negevensis-diagnostic-issues-identification-of-a-locus-encoding-a-rtx-toxin
#14
Onya Opota, Sacha Laurent, Trestan Pillonel, Marie Léger, Sabrina Trachsel, Guy Prod'hom, Katia Jaton, Gilbert Greub
Kingella kingae, producing the cytotoxic RTX protein, is a causative agent of serious infections in humans such as bacteremia, endocarditis and osteoarticular infection, especially in young children. Recently, Kingella negevensis, a related species, has been isolated from the oral cavity of healthy children. In this study, we report the isolation of K. negevensis strain eburonensis, initially misidentified as K. kingae with MALDI-TOF MS, from a vaginal specimen of a patient suffering of vaginosis. The genome sequencing and analysis of this strain together with comparative genomics of the Kingella genus revealed that K...
August 9, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/28801933/maldi-imaging-guided-microproteomic-analyses-of-heterogeneous-breast-tumors-a-pilot-study
#15
Deborah Alberts, Charles Pottier, Nicolas Smargiasso, Dominique Baiwir, Gabriel Mazzucchelli, Philippe Delvenne, Mark Kriegsmann, Daniel Kazdal, Arne Warth, Edwin De Pauw, Rémi Longuespée
PURPOSE: Matrix-assisted laser desorption/ionization (MALDI) imaging is an ideal tool to study intratumor heterogeneity (ITH) and its implication in prognostic stratification of patients. However there are some drawbacks concerning protein identification. On the other hand, laser microdissection (LMD)-based microproteomics allows retrieving thousands of protein identifications from small tissue pieces. As a proof of concept, we combined these two complementary approaches to analyze heterogeneous regions in breast tumors...
August 11, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28801836/optimization-of-sample-preparation-and-instrumental-parameters-for-the-rapid-analysis-of-drugs-of-abuse-in-hair-samples-by-maldi-ms-ms-imaging
#16
Bryn Flinders, Emma Beasley, Ricky M Verlaan, Eva Cuypers, Simona Francese, Tom Bassindale, Malcolm R Clench, Ron M A Heeren
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) has been employed to rapidly screen longitudinally sectioned drug user hair samples for cocaine and its metabolites using continuous raster imaging. Optimization of the spatial resolution and raster speed were performed on intact cocaine contaminated hair samples. The optimized settings (100 × 150 μm at 0.24 mm/s) were subsequently used to examine longitudinally sectioned drug user hair samples. The MALDI-MS/MS images showed the distribution of the most abundant cocaine product ion at m/z 182...
August 11, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28801830/cloning-and-expression-analysis-of-human-amelogenin-in-nicotiana-benthamiana-plants-by-means-of-a-transient-expression-system
#17
Mattia Pegoraro, Slavica Matić, Barbara Pergolizzi, Luca Iannarelli, Andrea M Rossi, Marco Morra, Emanuela Noris
Enamel is the covering tissue of teeth, made of regularly arranged hydroxyapatite crystals deposited on an organic matrix composed of 90% amelogenin that is completely degraded at the end of the enamel formation process. Amelogenin has a biomineralizing activity, forming nanoparticles or nanoribbons that guide hydroxyapatite deposit, and regenerative functions in bone and vascular tissue and in wound healing. Biotechnological products containing amelogenin seem to facilitate these processes. Here, we describe the production of human amelogenin in plants by transient transformation of Nicotiana benthamiana with constructs carrying synthetic genes with optimized human or plant codons...
August 11, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28801789/multimodal-laser-ablation-desorption-imaging-analysis-of-zn-and-mmp-11-in-breast-tissues
#18
Raquel González de Vega, María Luisa Fernández Sanchez, Noemí Eiro, Francisco J Vizoso, Michael Sperling, Uwe Karst, Alfredo Sanz Medel
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases. The main functions of these metalloproteinases are the degradation of the stromal connective tissue and basement membrane components. Recent data from model systems suggest that MMPs are involved in breast cancer (BC) initiation, invasion, and metastasis. Particularly, MMP-11 (stromelysin-3) is expressed in stromal fibroblasts adjacent to epithelial tumor cells, and high levels of this metalloproteinase were associated with tumor progression and poor prognosis of BC...
August 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801719/the-phospholipid-composition-of-kangaroo-spermatozoa-verified-by-mass-spectrometric-lipid-analysis
#19
Kathrin M Engel, Jürgen Schiller, Karin Müller, Dirk Dannenberger, Ulrike Jakop
Cryopreservation of kangaroo sperm has not been successful so far, and yet there is no promising cryopreservation protocol for these cells available. However, conservation of gametes is extremely important, particularly in the context of preserving endangered species. As spermatozoa are comprised of different membrane systems, the composition of these membranes might account for difficulties in cryopreservation. Lipids, as the main components, affect the physical properties of biological membranes and play a major role in sperm maturation...
August 11, 2017: Lipids
https://www.readbyqxmd.com/read/28801691/a-new-data-processing-routine-facilitating-the-identification-of-surface-adhered-proteins-from-bacterial-conditioning-films-via-qcm-d-maldi-tof-ms
#20
Siegfried Hohmann, Anke Neidig, Boris Kühl, Frank Kirschhöfer, Jörg Overhage, Gerald Brenner-Weiß
Conditioning films are an important factor in the initiation and development of microbial biofilms, which are the leading cause of chronic infections associated with medical devices. Here, we analyzed the protein content of conditioning films formed after exposure to supernatants of cultures of the human pathogen Pseudomonas aeruginosa PAO1. Adhesion of substances from the supernatant was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) sensor chips modified with the commonly used implant material titanium dioxide (TiO2)...
August 11, 2017: Analytical and Bioanalytical Chemistry
keyword
keyword
9717
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"